Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein P05067
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Human
Expected Species:
Bovine, Chicken, Dog, Porcine, Rabbit
Immunogen:
synthetic peptide chosen from human Abeta (1-42) protein, Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V-I-A
Applications:
Dot Blot (Dot), ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
The antibody can detect Abeta (1-42), Abeta (1-28) Abeta (1-20) and Abeta (1-17), This product exhibits a low reactivity to monomeric Abeta (1-42) as determined by SDS-PAGE and Western blotting,Immunolocalization: human tissue was paraffin-embedded and sectioned, De-waxed and rehydrated in an ethanol gradient, Antigens were retrieved in sodium citrate buffer (pH 6) at 95 C for 1 h, The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit
Application Details:
1 : 1000 (DB), 1 : 3000 (ELISA), 1-2 l/ml (IL)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
4,5 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Lindhagen-Persson et al. (2010). Amyloid-β oligomer specificity mediated by the IgM isotype--implications for a specific protective mechanism exerted by endogenous auto-antibodies. PLoS One. 2010 Nov 10;5(11):e13928. doi: 10.1371/journal.pone.0013928.
Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein P05067
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4 C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Human
Expected Species:
Bovine, Chicken, Dog, Porcine, Rabbit
Immunogen:
synthetic peptide chosen from human Abeta (1-42) protein, Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V-I-A
Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein P05067
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Human
Expected Species:
Bovine, Chicken, Dog, Porcine, Rabbit
Immunogen:
synthetic peptide chosen from human Abeta (1-42) protein, Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V-I-A
Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Human
Expected Species:
Primates, Chimpanzee, Gorilla
Immunogen:
recombinant, full length, human alpha-synuclein UniProt: P37840, epitope was mapped between amino acid 1-15
Applications:
ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
Bargar et al. (2021) Discrimination of MSA-P and MSA-C by RT-QuIC analysis of olfactory mucosa: the first assessment of assay reproducibility between two specialized laboratories. Mol Neurodegener. 2021 Dec 11;16(1):82. doi: 10.1186/s13024-021-00491-y. PMID: 34895275; PMCID: PMC8665327.Br nnstr m et al. (2014). A generic method for design of oligomer-specific antibodies. PLoS One. 2014 Mar 11;9(3):e90857. doi: 10.1371/journal.pone.0090857. eCollection 2014.
Special application note:
Immunolocalization: human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95 C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit.This antibody will recognize human SNCA monomers and multimers in Western blot and can be used to detect fibrills in a sandwich ELISA. In ELISA this antibody can be used for detection, combined with AS13 2719 as a capture antibody.
Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species. Protein tyrosine nitration results in a post-translational modification, component of nitric oxide signaling.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivitywith non-nitrated tyrosine. Not species specific.0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot..ECL.Antibody works paraffin-embedded sections.
Application Details:
1: 100 (IHC), 1: 1400 (WB), The exact and optimal working dilution should be determined by the investigator
Conjugation:
IgG2A
Isotype:
IgG2A
Purity:
Total IgG. Protein G purified, in PBS. Contains 50 % glycerol and 0.09% sodium azide.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.Antibody used in immunohistochemistry:Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387.
Special application note:
1 mg/ml of Protein G purified IgG2A in PBS pH 7,4, 0,09 % sodium azide, 50 % glycerol
Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species. Protein tyrosine nitration results in a post-translational modification, component of nitric oxide signaling.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivity with non-nitrated tyrosine. Not species specific.0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot.Antibody works paraffin-embedded sections.
Application Details:
1: 100 (IHC), 1: 1400 (WB), The exact and optimal working dilution should be determined by the investigator
Conjugation:
IgG2A
Isotype:
IgG2A
Purity:
Total IgG. Protein G purified, in PBS. Contains 50 % glycerol and 0.09% sodium azide.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.Antibody used in immunohistochemistry:Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387.
Special application note:
1 mg/ml of Protein G purified IgG2A in PBS pH 7,4, 0,09 % sodium azide, 50 % glycerol
Soluble oligomeric assemblies of the Amyloid-β peptide are today anticipated to be the direct cause regarding the Alzheimer pathology. As a consequence, oligomeric Aβ-assemblies constitute a very interesting therapeutic target. Identification of Aβ-oligomers is however, technically challenging due to there labile nature and low abundance. Abeta oligomer-specific OMAB antibody is based on the IgM isotype and represents a new concept of Aβ-oligomer binders using a combination of high avidity and very low monovalent affinity. This combination creates a selectivity of the antibody towards the oligomeric fraction and minimizes reactivity towards monomeric species.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human Abeta oligomers only
Expected Species:
Rat
Immunogen:
partly aggregated, recombinant peptide corresponding to the human Abeta (1-40/42), Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V, The epitope is 3-8, Molecular weight of immunogen is 4,5 kDa,
OMAB antibody is a versatile tool within research of Alzheimer’s disease, A sandwhich ELISA illustrates its potential regarding its high selectivity towards A? oligomers
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Pang et al (2021) An App knock-in rat model for Alzheimer's disease exhibiting A? and tau pathologies, neuronal death and cognitive impairments. Cell Res. 2021 Nov 17. doi: 10.1038/s41422-021-00582-x. Epub ahead of print. PMID: 34789895.Oh et al. (2020). Associative Interactions among Zinc, Apolipoprotein E, and Amyloid-? in the Amyloid Pathology. Int J Mol Sci. 2020 Jan 25;21(3). pii: E802. doi: 10.3390/ijms21030802.Henning-Knechtel et al. (2020). Designed Cell-Penetrating Peptide Inhibitors of Amyloid-beta Aggregation and Cytotoxicity. Cell Reports Physical Science,Volume 1, Issue 2, 26Zhang et al. (2019). Brains of rhesus monkeys display A? deposits and glial pathology while lacking A? dimers and other Alzheimer's pathologies. Aging Cell. 2019 Jun 4:e12978. doi: 10.1111/acel.12978.Kumar et al. (2018). Peptidomimetic-Based Multidomain Targeting Offers Critical Evaluation of A? Structure and Toxic Function. J Am Chem Soc. 2018 May 30;140(21):6562-6574. doi: 10.1021/jacs.7b13401.
Special application note:
OMAB antibody has been purified by by ion-exchange chromatography and is supplied in PBS without any additives as carrier proteins or sodium azide.Binding of OMAB antibody and Abeta oligomers at RT takes about 15 min.Fibrils are inaccessible for OMAB antibodies therefore if a discrimination between fibrils and oligomers is to be achieved, dot blot can be used. Start with antigen concentration of 500 ng/dot followed by 2X dilution steps. Blocking: non-fat milk and washes with 0.3 % Tween 20 in TBS pH 7.4.
Soluble oligomeric assemblies of the Amyloid-β peptide are today anticipated to be the direct cause regarding the Alzheimer pathology. As a consequence, oligomeric Aβ-assemblies constitute a very interesting therapeutic target. Identification of Aβ-oligomers is however, technically challenging due to there labile nature and low abundance. Abeta oligomer-specific OMAB antibody is based on the IgM isotype and represents a new concept of Aβ-oligomer binders using a combination of high avidity and very low monovalent affinity. This combination creates a selectivity of the antibody towards the oligomeric fraction and minimizes reactivity towards monomeric species.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human Abeta oligomers only
Expected Species:
Rat
Immunogen:
partly aggregated, recombinant peptide corresponding to the human Abeta (1-40), Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V
OMAB antibody is a versatile tool within research of Alzheimer’s disease, A sandwhich ELISA illustrates its potential regarding its high selectivity towards A? oligomers
Application Details:
Coating antibody at 2 g/ml (ELISA), 1 : 500 (IHC)
Conjugation:
IgM
Isotype:
IgM
Purity:
Affinity purified in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 l of sterile water
Molecular Weight:
4,5 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Richman et al. (2013). In Vitro and Mechanistic Studies of an Anti-Amyloidogenic Self-Assembled Cyclic D,L-#-Peptide Architecture. J. Americal Chemical Societ, Jan 19.Lindhagen-Persson et al. (2010). Amyloid-β Oligomer Specificity Mediated by the IgM Isotype – Implications for a Specific Protective Mechanism Exerted by Endogenous Auto-Antibodies. PLoS ONE.
Special application note:
OMAB antibody has been purified by by ion-exchange chromatography and is supplied in PBS without any additives as carrier proteins or sodium azide. Binding of OMAB antibody and Abeta oligomers at RT takes about 15 min.Fibrils are inaccessible for OMAB antibodies therefore if a discrimination between fibrils and oligomers is to be achieved, dot blot can be used. Start with antigen concentration of 500 ng/dot followed by 2X dilution steps. Blocking: non-fat milk and washes with 0.3 % Tween 20 in TBS pH 7.4.
Ergotamine is a secondary metabolite (natural product) and the principal alkaloid produced by the ergot fungus, Claviceps purpurea, and related fungi in the family Clavicipitaceae. It possesses structural similarity to several neurotransmitters, and has biological activity as a vasoconstrictor. It is used medicinally for treatment of acute migraine attacks (sometimes in combination with caffeine), and to induce childbirth and prevent post-partum haemorrhage.
Halorhodopsin (HR) is a hyperpolarizing light-driven ion pump from the halophilic archaea bacterium Natronomonas pharaonis. HR uses the energy of yellow light (excitation maximum near 580 nm) to mediate primarily chloride but also bromide, iodide, and nitrate import into the cell against their electrochemical gradients.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Natronomonas pharaonis
Expected Species:
Natronomonas pharaonis Species of your interest not listed? Contact us
Immunofluorescence was performed on mouse brain slices (data not shown)
Application Details:
1 : 200 (IF), 1 : 1000-1 : 20 000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
30,9 | 30 kDa
Not reactive in:
Halobacterium salinarum (HsHR and HsBR)
Selected references:
Alfonsa et al. (2015) The contribution of raised intraneuronal chloride to epileptic network activity. J Neurosci. 2015 May 20;35(20):7715-26. doi: 10.1523/JNEUROSCI.4105-14.2015.
Lycopene cyclase (EC=5.5.1.19) is an enzyme involved in carotenoid biosynthesis, which catalyzes the double cyclization reaction which converts lycopene to beta-carotene and neurosporene to beta-zeacarotene. Synonymes:
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tang el al. (2020). OsNSUN2-Mediated 5-Methylcytosine mRNA Modification Enhances Rice Adaptation to High Temperature. Dev Cell. 2020 May 4;53(3):272-286.e7. doi: 10.1016/j.devcel.2020.03.009.Sun et al. (1998). Differential expression of two isopentenyl pyrophosphate isomerases and enhanced carotenoid accumulation in a unicellular chlorophyte. PNAS, Vol. 95, pp. 11482–11488, September 1998.
Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein. Recent findings however suggest that smaller oligomeric forms of Abeta, formed in parallel to the amyloid plaques, excert the predominant tissue damaging effect.Specific identification of the oligomeric forms is as a consequence of great interest. Based on a recently published technique a highly oligomer-specific antibody (mAB-O), targeting Abeta oligomers while omitting reactivity towards the monomeric and fibrillar counterpart, has been developed.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human
Expected Species:
Mouse, Bovine, Chicken, Dog, Porcine, Rabbit
Immunogen:
Synthetic peptide chosen from human Abeta (1-42) protein,
Applications:
Dot blot (Dot), ELISA (ELISA), Immunolocalization (IL)
Immunolocalization: human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95 C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit.This Monoclonal IgG1, kappa light chain, (clone number 3E5.F8) is specific for human Amyloid-Beta oligomers.
Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein. Recent findings however suggest that smaller oligomeric forms of Abeta, formed in parallel to the amyloid plaques, excert the predominant tissue damaging effect.Specific identification of the oligomeric forms is as a consequence of great interest. Based on a recently published technique a highly oligomer-specific antibody (mAB-M), targeting Abeta oligomers while omitting reactivity towards the monomeric and fibrillar counterpart, has been developed.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
For short time storage please add sodium azide and srote at +4°C.For long time storage store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
synthetic peptide chosen from human Abeta protein (3-10) pregion, oligomer specific
Applications:
Dot blot (Dot), ELISA (ELISA), Immunolocalization (IL)
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Meilandt et al. (2019). Characterization of the selective in vitro and in vivo binding properties of crenezumab to oligomeric A ?². Alzheimers Res Ther. 2019 Dec 1;11(1):97. doi: 10.1186/s13195-019-0553-5.Br nnstr m et al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.
Special application note:
Immunolocalization: human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95 C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit.This antibody is specific for human Amyloid-Beta oligomers.
Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy. In analogy to many other amyloid associated disorders, alpha-synuclein may also form oligomeric assemblies. These small and soluble forms have been suggested to exert a stronger tissue damaging effect as compared to the monomeric and fibrillar counterpart. Using a recently developed technique a monoclonal oligomer-specific antibody has been designed.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
For short time storage please add sodium azide and srote at +4°C.For long time storage store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human
Expected Species:
Mouse
Immunogen:
synthetic peptide derived from human alpha-synuclein Glu131-Ala140
Applications:
Dot blot (Dot), ELISA (ELISA), Immunolocalization (IL)
Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy. In analogy to many other amyloid associated disorders, alpha-synuclein may also form oligomeric assemblies. These small and soluble forms have been suggested to exert a stronger tissue damaging effect as compared to the monomeric and fibrillar counterpart. Using a recently developed technique a monoclonal oligomer-specific antibody has been designed.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
For short time storage add sodium azide and store at +4°C. For long time storage store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Synthetic peptide derived from human alpha-synuclein Glu131-Ala140
Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy. In analogy to many other amyloid associated disorders, alpha-synuclein may also form oligomeric assemblies. These small and soluble forms have been suggested to exert a stronger tissue damaging effect as compared to the monomeric and fibrillar counterpart. Using a recently developed technique a monoclonal oligomer-specific antibody has been designed.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
For short time storage add sodium azide and store at +4°C. For long time storage store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human, mouse
Immunogen:
Synthetic peptide derived from human alpha-synuclein Gly111-Tyr125
Applications:
Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC)
Limegrover et al. (2021) Sigma-2 receptor antagonists rescue neuronal dysfunction induced by Parkinson's patient brain-derived a-synuclein. J Neurosci Res. 2021 Apr;99(4):1161-1176. doi: 10.1002/jnr.24782. Epub 2021 Jan 22. PMID: 33480104.Kilpel inen et al. (2019). Behavioural and dopaminergic changes in double mutated human A30P*A53T alpha-synuclein transgenic mouse model of Parkinson s disease.Sci Rep. 2019 Nov 22;9(1):17382. doi: 10.1038/s41598-019-54034-z.Wu et al. (2017). The critical role of Nramp1 in degrading a-synuclein oligomers in microglia under iron overload condition. Neurobiol Dis. 2017 Aug;104:61-72. doi: 10.1016/j.nbd.2017.05.001. (human, mouse, immunolocalization)Svarcbahs et al. (2016). Inhibition of Prolyl Oligopeptidase Restores Spontaneous Motor Behavior in the a-Synuclein Virus Vector-Based Parkinson's Disease Mouse Model by Decreasing a-Synuclein Oligomeric Species in Mouse Brain. J Neurosci. 2016 Dec 7;36(49):12485-12497.Br nnstr m et al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.
Special application note:
This antibody is specific to oligomers in ELISA as a capture antibody. For specific details, please check: Br nnstr m et al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.
Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy. In analogy to many other amyloid associated disorders, alpha-synuclein may also form oligomeric assemblies. These small and soluble forms have been suggested to exert a stronger tissue damaging effect as compared to the monomeric and fibrillar counterpart. Using a recently developed technique a monoclonal oligomer-specific antibody has been designed.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Synthetic peptide derived from human alpha-synuclein Gly111-Tyr125
Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
For short time storage add sodium azide and store at +4°C. For long time storage store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human
Expected Species:
Mouse
Immunogen:
synthetic peptide derived from human alpha-synuclein N-terminal Met1-Val15
Applications:
Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC)
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Tanudjojo et al. (2021) Phenotypic manifestation of ?-synuclein strains derived from Parkinson's disease and multiple system atrophy in human dopaminergic neurons. Nat Commun. 2021 Jun 21;12(1):3817. doi: 10.1038/s41467-021-23682-z. PMID: 34155194; PMCID: PMC8217249.
Special application note:
This antibody will recognize human SNCA monomers and multimers in Western blot. This antibody binds weakly to fibrills in IHC.Cross reactivity of this antibody to synuclein beta was not determined.This antibody can be used as a capture antibody in ELISA, combined with AS08 358 as a detection antibody.
Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy. In analogy to many other amyloid associated disorders, alpha-synuclein may also form oligomeric assemblies. These small and soluble forms have been suggested to exert a stronger tissue damaging effect as compared to the monomeric and fibrillar counterpart. Using a recently developed technique a monoclonal oligomer-specific antibody has been designed.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
For short time storage add sodium azide and store at +4°C. For long time storage store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
synthetic peptide derived from human alpha-synuclein N-terminal Met1-Val15
Acrylamide is a starting material for the production of polyacrylamide, that in turn is used as a filler and in the treatment of waste water, gel electrophoresis, paper production. Acrylamide is produced during heating of foods and it is found in coffee and other food products. The compound is neurotoxic and possesses endocrine disrupting properties.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cup to one month or in aliquots at -20 °C for long time storage. Avoid repeated freezing and thawing.
Total chicken immunoglobulin antibody fraction (IgY) is present in phosphate buffered saline, pH 7.2, 0.02% sodium azide as preservative.The antibody may be used in immunoaffinity chromatography for the isolation, purification and concentration of acrylamide from food samples for further analysis.
LCY (lycopene beta-cyclase) is an enzyme (EC:5.5.1.19) of beta carotene biosynthesis pathway. Catalyzes the double cyclization reaction which converts lycopene to beta-carotene and neurosporene to beta-zeacarotene. Protein is encoded by LCY1 gene.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Transthyretin is a carrier protein in plasma of the thyroid hormone. This protein forms a complex with retinol-binding protein and it has the capability of forming amyloid fibrils. 25% of individuals older than 80 years are affected by senile systemic amyloidosis and most cases of TTR-associated amyloidosis are linked to point mutations. A substitution of valine for methionine at position 30 of the 127-aa-long polypeptide is one of the most common forms, leading to many symptoms in the peripheral nervous system, a familial amyloidosis with polyneuropathy.This monoclonal IgG1 antibody is amyloid specific for human Transthyretin. Detects the N-terminal fragment TTR-1-48 frequently formed in vivo.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
For short time storage please add sodium azide and srote at +4°C.For long time storage store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Full length variant TTR,
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Goldsteins et al. (1999). Exposure of cryptic epitopes on transthyretin only in amyloid and in amyloidogenic mutants. Proc Natl Acad Sci U S A. 1999 Mar 16; 96(6): 3108–3113
Transthyretin (TTR), formerly known as Prealbumin, is in vivo involved in the binding and transportation of the Thyroxin hormone and retinol-binding protein. Mutations in TTR are associated with familial amyloidotic polyneuropathy (FAP) which is a fatal disease characterized by amyloid depositions found in visceral organs including the heart, liver, and kidney. The wild type form of TTR is associated with a late onset amyloidosis denoted senile systemic amyloidosis, affecting around 10% of the population above 80 years of age with depositions mainly found in the heart.Monoclonal IgG1 antibody. Amyloid specific for human Transthyretin. Detects the C-terminal fragment 49-127 frequently formed in vivo.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at 4 C, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human Transthyretin Amyloids
Immunogen:
Recombinant protein corresponding to the Human wild type Transthyretin. GPTGTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGKTSESGELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEHAEVVFTANDSGPRRYTIAALLSPYSYSTTAVVTNPKE The epitope has been mapped to residue 56-61
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Specifically reactive to the amyloid form of human Transthyretin. Epitope mapped to residue 56-61 which remains buried within the native fold of transthyretin but becomes exposed within its amyloid form.It has been suggested that that two distinct mechanisms of TTR-amyloidosis exists. The first, most common seen in wild type TTR Amyloidosis, consists of the full length TTR. Whereas the other type of amyloidosis mainly consists of the C-terminal region of the protein and is more common in mutant versions of TTR. Mouse IgG1 Anti-Transthyretin 56-61 (Amyloid Specific) epitope is located at the C-terminal strand of cleaved TTR and is suitable to detect amyloid formation derived from the C-terminal.
Application Details:
1:1000 (ELISA), 1:500 (IHC), 1:1000 (WB)
Conjugation:
IgG1
Isotype:
IgG1
Purity:
Affinity purified in PBS pH 7.4.
Reconstitution:
Add 100 µl sterile water to reconstitute to 1 mg/ml
Molecular Weight:
155
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Goldsteins et al. (1999). Exposure of cryptic epitopes on transthyretin only in amyloid and in amyloidogenic mutants. Proc Natl Acad Sci U S A. 1999 Mar 16; 96(6): 3108–3113
5-hmC | 5-hydroxymethylcytosine is a recently discovered DNA modification which results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. It may have an important roles distinct from that of 5-methylcytosine (5-mC). 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; for long term storage -80°Cis recommened; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Close structural similarity between 5-mC and 5-hmC makes them very difficult to expreimentally distinuish. Therefore affinity-based technology was employed to purify 5-hmC specific antibodies. This antibody is affinity purified and provided in PBS pH 7.4 with 0.05 % sodium azide.
5-hmC | 5-hydroxymethylcytosine is a recently discovered DNA modification which results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. It may have an important roles distinct from that of 5-methylcytosine (5-mC). 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6% of total nucleotides in Purkinje cells.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; for long term storage Store at -80 C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
This serum contains 0,05 % sodium azide
Research area:
Plant Epigenetics/DNA methylation
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