Newmarket Scientific
Newmarket Scientific








Cell Signalling


Antibodies and reagents for cell signalling


NS Reagents logo   Affinity Biosciences logo  

 


 

Our cell signalling range includes around 5000 Phospho-Specific antibodies as well as thousands of other cell signalling/signal transduction related antibodies. Plus, as with every antibody we sell they all come with our 100% antibody guarantee.

 

There are too many antibodies to list on one page, so please use the search box above to find a specific product or simply contact us and we'll find it for you.

 



Affinity Biosciences - The phospho-antibody specialist

 

Affinity Biosciences is a provider of innovative tools for signal transduction research with a focus on well validated phospho-specific antibodies.

 

Use our search to find your antibody (e.g. Ser724, IRE1 or Phospho-IRE1 etc).

 

Well validated phospho-specific antibodies

Validation is important for any antibody, but for Phospho-specific antibodies it is even more important as specificity is truly key to their usefulness.

 

Affinity Biosciences antibody validation begins with Mass Spec (MS) analysis of the blocking peptide to ensure the peptide has been produced correctly, followed by checking that the peptide blocks the antibody well in Western blotting with research samples showing that the antibody corresponds correctly to the blocking peptide.

 

DATASHEET IMAGES: Images of Western blots are provided on the datasheets showing blocking and control lanes (without the peptide) to show binding of the target along with images of other applications such as Immunohistochemistry, immunofluorescence etc as appropriate.

 

As an example, details of the validation of our Phospho-IRE1 (Ser724) - AF7150 antibody have been provided below:

 

Western Blot, IHC, Immunofluorescence and Peptide-ELISA for Anti Phospho-IRE1 (Ser724).

 

pIRE1 WB  pIRE1 IHC

pIRE1 Immunofluorescence  pIRE1 ELISA

Phospho-IRE1 (Ser724) - AF7150 Datasheet

 

Affinity also produce more than 500 inhibitors and SiRNAs.

 

Additionally all affinity products are covered by our 100% antibody guarantee, so you can try them without worrying whether they will work. We know they work well, but we understand you may be sceptical having tried other antibodies in the past, so now you can try them without worrying.

If they don't work, we'll give you your money back.

 

 

Other Highlighted Antibodies:

 

Anti-Phospho-ERK1/2 Antibody (Thr202/Tyr204) - AF1015

29 Citations

 

Anti-Phospho-Akt Antibody (Ser473) - AF0016

28 Citations

 

Phospho-mTOR Antibody (Ser2448) - AF3308

14 Citations 

 

Phospho-p70 S6 Kinase Antibody (Thr389/412) - AF3228

9 Citations

 

Use our search at the top of the page to find your antibody

(e.g. Ser724, IRE1 or Phospho-IRE1 etc).

 

Questions? Email us at tech@nktscientific.com.

 



Anti-RAMP Antibodies (1, 2 and 3)

 

Receptor Activity Modifying Proteins (RAMPs) are required to transport calcitonin-receptor-like receptor (CRLR or CALCRL) to the plasma membrane. CRLR is a G protein-coupled receptor receptor with seven transmembrane domains, which can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. 

 

Wth RAMP1 it produces a CGRP receptor with RAMP2 it produces an adrenomedullin (AM) receptor (AM1) with RAMP3 it produces a dual CGRP/AM receptor (AM2)

 

In general four roles have been reported for RAMPs:

1. RAMPs enable the forward-trafficking of some GPCRs to the cell surface.

2. RAMPs can alter GPCR pharmacology, switching ligand selectivity for some GPCRs.

3. RAMPs can influence coupling to GPCR signaling pathways.

4. RAMPs may alter the trafficking pathway of some GPCRs from the cell surface, with different RAMPs controlling receptor fate through recycling or degradative pathways.

 

We have 3 antibodies available as follows:

 

Anti-RAMP1 Antibody         

Applications: WB, IHC  

Host: Rabbit     Reactivity: Human, Mouse, Rat  

 

Anti-RAMP2 Antibody         

Applications: WB  

Host: Rabbit     Reactivity: Human, Mouse, Rat  

 

Anti-RAMP3 Antibody         

Applications: WB, IF  

Host: Rabbit     Reactivity: Human, Mouse, Rat  

 

All are available as 50ug or 100ug size

 

More information is available on the links above or complete the form below to request copies of the datasheets.

 


 

Anti-DNA damage-binding protein 1

 

DNA damage-binding protein 1 (DDB1) is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex. DDB1 also functions as a core component of the cullin 4 (CUL4) ubiquitin E3 ligase complex, facilitating the binding of substrates to this complex and the ubiquitination of proteins. These factors (ubiquitin ligase substrates) regulate numerous essential processes in the cell including DNA repair (DDB2), DNA replication, chromatin remodelling (Cdt2) and more.

 

Applications: WB           Host: Rabbit      Reactivity: Human

 



Submit this form to request datasheets for these antibodies



Secondary antibodies

Secondary Antibodies

 

Our range of secondary antibodies includes antibodies to many primary antibody species:

 

Anti-Human, Anti-Mouse, Anti-Rabbit, Anti-Goat, Anti-Rat, 
Anti-Sheep, Anti-Guinea Pig, Anti-Hamster, Anti-Bovine, 
Anti-Donkey, Anti-Cat, Anti-Dog, Anti-Llama and Anti-Chicken

 

All secondary antibodies are available as a range of conjugates including Dylight Dyes, HRP, AP, FITC, TRITC and more. 

 

We can provide quantities from milligrams (mg) right up to grams (g) for bulk antibody requirements.

 

Follow the link above to select your secondary antibody from a list of options or if you are having difficulty locating it, simply use our contact form or email us at tech@nktscientific.com.