Newmarket Scientific
Newmarket Scientific








Neuroscience Research - ALS and FTD Focus


Antibodies and reagents for Amyotrophic lateral sclerosis (ALS) and Frontotemporal dementia (FTD)


Affinity Biosciences logo   Agrisera logo     Biosensis logo   ImmunoStar logo  NS Reagents logo  

 


 

Newmarket Scientific provides products for neuroscience research with a focus on Amyotrophic lateral sclerosis (ALS) and Frontotemporal dementia (FTD):

 

Antibodies, Proteins, ELISA kits and Related reagents.

 

Some products for Amyotrophic lateral sclerosis (ALS) research and Frontotemporal dementia (FTD) research are highlighted below, but many more are available, so please use the search box above to find other products in our neuroscience range or if there is something specific you are looking for you can email us at tech@nktscientific.com

 

Also for more information on neuroscience research in general, including highlighted articles/papers and other resources check out our .

 



TDP43 Antibodies

TAR DNA-binding protein 43 (TDP43) binds both DNA and RNA and has multiple functions in transcriptional repression, pre-mRNA splicing and translational regulation. Characterisation of transcriptome-wide binding sites shows that thousands of RNAs are bound by TDP43 in neurons.

 

Hyperphosphorylated, fragmented and ubiquitinated forms of TDP-43 have been identified as core components of cytosolic inclusions in sporadic ALS (amyotrophic lateral sclerosis) and FTLD (frontotemporal lobar degeneration). As well as ALS and FTLD, mutations in TDP-43 have also been associated Parkinson's disease and Alzheimer's disease. 

 

TDP43 is a key target protein in research around numerous neurodegenerative conditions. Read More...

 

NS Reagents TDP43 Antibody (Cat-AA17-100105) Image 1 NS Reagents TDP43 Antibody (Cat-AA17-100105) Image 2

 

ALS patient line showing proteinopathy.

 

 

TDP43: Cat No: AX17-10010

Lot AX17-1808-0002

Technique: ICC

Secondary Ab: Alexa 488

Primary Ab dilution: 1:200

 


 

Affinity TDP43 Phospho (Cat-AF7365) Image 3 Affinity TDP43 Phospho (Cat-AF7365) Image 2

 

ALS patient line showing

nuclear TDP43 phosphorylation.

 

Affinity - phospho TDP43 Ab Cat No: AF7365

Lot 19p1575

Technique: ICC

Secondary Ab: Alexa 488

Primary Ab dilution: 1:100

 


Images courtesy of Dr Laura Ferraiuolo and Mr Marco Destro at the University of Sheffield

 



RNA-binding Proteins and Stress granules in ALS-FTD

Cytoplasmic stress granules are membrane-less aggregates that form through liquid phase separation as a protective response to physiological or pathological conditions such as oxidative stress, hypoxia or virus infection. They are transient and usually disappear after the stress is removed.

 

Stress granules are mainly composed of messenger RNAs (mRNAs) stalled in translation initiation, translation initiation components such as eukaryotic initiation factor 4G (eIF4G), RNA-binding proteins (RBPs) and ribonucleoproteins. Formation of stress granules can be initiated by the Ras GTPase-activating protein-binding protein 1 (G3BP1), which is commonly used as a stress granule marker.

 

Mutations in genes that encode RNA binding proteins (RBPs) such as FUS/TLS, TDP-43, Ataxin 2, TAF15, EWSR1, hnRNPA1 and hnRNPA2, MATR3 and TIA-1 have recently been linked to ALS/FTD. Some of these RNA binding proteins contain low complexity sequence domains (LCDs) that have been shown to mediate the phase separation. Mutations found in these domains increase the propensity of RNA binding proteins to aggregate and form pathological amyloid-like fibrils in the cell bodies.

 

Related products:

USP10   Caprin1    HDAC6    eIF4A3

 

TDP43 - TAR DNA-binding protein 43: A key protein in ALS and FTLD, Parkinson's and Alzheimer's 

 

References:

References: RNA-Binding Proteins in Amyotrophic Lateral Sclerosis, Zhao M. et al.

Mol Cells. 2018 Sep 30;41(9):818-829  

 

Decoding ALS: From Genes to Mechanism, Taylor JP et al.

Nature. 2016 Nov 10; 539(7628): 197–206.

 

Relationships between Stress Granules, Oxidative Stress, and Neurodegenerative Diseases Chen L et al.  Oxidative Medicine and Cellular Longevity, Volume 2017, Article ID 1809592

 



Nerve Growth Factor Receptor (p75NTR)

The nerve growth factor (NGF) receptor, also known as p75 neurotrophin receptor (p75NTR), is a low affinity NGF receptor. The other members of the neurotrophin family, including brain-derived neurotrophic factor (BDNF), neurotrophin (NT)-3 and NT-4/5 bind to p75NTR with equal affinity. This receptor also binds pro-neurotrophins. p75NTR mediates signalling of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Altered neurotrophin levels and p75NTR expression are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS).

 

Our new p75NTR antibody (clone 8J2) to the extracellular domain of p75NTR is ideal for:

 

- In vivo targeting: retrograde tracing and receptor trafficking

- Flow cytometry, immunopanning, western blot, immunohistochemistry and more.

- Most mammalian species, including human, mouse and rat

- Replacing clone MLR2 (A comparative study can be viewed here).

 

p75NTR antibody (clone 8J2)

 

Related products:

NGFR/p75ECD Rapid ELISA Kits (Human or Mouse),

Antibodies to p75NTR

NT3 Kits and Antibodies

NT4/5 Kits and Antibodies