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Neuroscience Research - Parkinson's Focus


Antibodies and reagents focussing on Parkinson's disease research


Affinity Biosciences logo   Agrisera logo     Biosensis logo   ImmunoStar logo  NS Reagents logo  

 


 

Newmarket Scientific provides products for neuroscience research with a focus on Parkinson's disease:

 

Antibodies, Proteins, ELISA kits and Related reagents.

 

Some products for Parkinson's disease research are highlighted below and more information on neuroscience research in general, is available on our Neuroscience Twitter feed , including highlighted articles/papers and other resources.

 



Phospho-synuclein antibodies from Affinity

Alpha-synuclein is a 140 amino acid protein which, in humans, is encoded by the SNCA gene. It is expressed predominantly in the brain and particularly in presynaptic nerve terminals. Due to its structural flexibility, alpha-synuclein can adopt several conformations and depending on the environment and the binding partners, exists as a dynamic balance between monomeric unfolded and an amphipathic alpha-helix (membrane binding) states.

 

In pathological conditions such as Parkinson’s disease, soluble alpha-synuclein monomers associate to form large and insoluble aggregates, the main component of Lewy body inclusions. These neurotoxic aggregates cause the progressive degeneration of dopaminergic neurons in the substantia nigra pars compacta, and are responsible for the motor symptoms in PD. In particular, post-translational modifications of alpha-synuclein can lead to a change in conformation resulting in the alpha-synuclein proteins being more susceptible to aggregation. In Lewy bodies, it has been shown that phosphorylated Ser129 alpha-synuclein is the most abundant form of alpha-synuclein present in these aggregates.

 

Phospho-specific alpha-synuclein Antibodies

 

Related products:

Affinity phospho-synuclein (Ser129)

Affinity phospho-synuclein alpha (Tyr 133)

Affinity phospho-synuclein alpha (Tyr 136)

All 3 phospho-synuclein antibodies

 

Biosensis alpha-synuclein Rapid ELISA Kit (BEK-2216-1P/2P)

Biosensis chicken alpha-synuclein antibody

Biosensis sheep anti alpha-synuclein antibodies

Biosensis rabbit anti alpha-synuclein antibody

 



Biosensis chicken α-synuclein antibody

Our chicken anti alpha-synuclein antibody from Biosensis provides a very clear detection signal with minimal background in Western Blot,  Immunocytochemistry, Immunohistochemistry applications AND because it is raised in chicken it can be used alongside other antibodies raised in mouse or rabbit for multiple staining work.

 

• Raised against the non-amyloid β component (NAC, 61-95) region of α-synuclein

• Validated for WB, ICC and IHC

• Superb signal with clean background

• Perfect partner for rabbit and mouse antibodies in double or triple labelling experiments

• Detects alpha-synuclein in human, mouse, rat, and numerous other species

Biosensis alpha-synuclein ELISA Kit

Left: Rat cerebellum section stained with chicken anti-α-synuclein antibody (red, 1:3,000) by Immunohistochemistry. Section was co-stained with rabbit anti-GFAP (green, R-1374-50, 1:5,000) and DAPI nuclear dye (blue). IHC method: Following transcardial perfusion with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 uM sections, and free-floating sections were stained. The α-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells.

 

Right: Western blot analysis of tissue lysates. Lane 1: MWM; Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord. Antibody dilution: 1:2,000. A strong band at about 15 kDa corresponds to the α-synuclein protein in brain extracts, which are rich in synapses.

 

Related products:

Biosensis alpha-synuclein Rapid ELISA Kit (BEK-2216-1P/2P)

Biosensis sheep anti alpha-synuclein antibodies

Biosensis rabbit anti alpha-synuclein antibody

Phospho-synuclein antibodies

 



New: Biosensis α-synuclein RapidTM ELISA Kit

The Biosensis alpha-synuclein Rapid ELISA Kit (BEK-2216-1P/2P) features:

 

• Real validation data on accurate quantification in human blood and CSF

• Low intra- and inter-assay CVs of < 10%

• Complete kit with precise instructions

• Fast (< 4 hours) and reliable assay performance.

 

Until 31st Jan 2020 Buy a 2-plate kit for the price of a 1-plate kit Code: "Synuclein120"

 

Biosensis alpha-synuclein ELISA Kit

 

Related products:

Biosensis chicken alpha-synuclein antibody

Biosensis sheep anti alpha-synuclein antibodies

Biosensis rabbit anti alpha-synuclein antibody

Phospho-synuclein antibodies

 



The role of the retromer component VPS-35 in neurodegenerative diseases

Cargoes, originating from the plasma membrane or the biosynthesis pathways and entering the endosomal systems, are either retained in endosomes for subsequent lysosomal degradation or are exported from the endosomes for reuse or recycling. Endosomes are therfore important protein sorting stations contributing to cell homeostasis and deficiencies in the endosomal sorting system have been linked to neurodegenerative diseases such as Alzheimer’s and Parkinson’s.

 

Retromer is an evolutionarily conserved endosomal-associated-protein known to play an essential role in the retrograde transport of transmembrane proteins from the endosomes back to the trans-Golgi network or to the plasma membrane via the recycling pathway. Retromer is a pentameric complex composed of a cargo-selective trimeric complex, with the major subunit being VPS-35 (vacuolar protein sorting 35) which is combined with a sorting nexin dimer.

 

The endosomal retromer is known to bind to and recruit the Wiskott–Aldrich syndrome and SCAR homolog (WASH) complex to the endosomal membranes. The latter helps the formation of actin patches that facilitate protein sorting. It is thought that mutations in the VPS-35 gene weaken the binding of VPS-35 with an element of the WASH complex, which consequently results in the abnormal protein sorting. The D620N mutation in VPS-35 has been associated with Parkinson’s disease.


NS Reagents VPS-35 antibodies:

NS Reagents Anti-VPS35 antibody AB19-10109

Applications: WB | IHC Reactivity: Hu | Ms

 

NS Reagents Anti-VPS35 antibody AB19-10110 (N Terminal)

Applications: WB Reactivity: Hu | Ms | Rt

 

References:

Parkinson’s disease-linked D620N VPS35 knockin mice manifest tau neuropathology and dopaminergic neurodegeneration, Chen X et al, Proc Natl Acad Sci U S A. 2019 Mar 19; 116(12): 5765–5774. 


The functional roles of retromer in Parkinson's disease, Cui Y et al, FEBS Lett. (2018) 


The retromer complex – endosomal protein recycling and beyond Seaman M.N. J, J Cell Sci. 2012 Oct 15; 125(20): 4693–4702. doi: 10.1242/jcs.103440 


Retromer: A Master Conductor of Endosome Sorting, Burd C et al, Cold Spring Harb Perspect Biol. 2014 Feb; 6(2): a016774. doi: 10.1101/cshperspect.a016774 


Retromer-mediated endosomal protein sorting: all WASHed up! Seaman MNJ et al, Trends Cell Biol. 2013 Nov;23(11):522-8. doi: 10.1016/j.tcb.2013.04.010. Epub 2013 May 28. 

 



Neurofilaments

Neurofilaments (NF) are the major constituent of the neuronal cytoskeleton. They are composed of a mixture of subunits which principally include the neurofilament triplet proteins NF-L (light), NF-M (medium) and NF-H (heavy). Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. A role of neurofilaments in a variety of neurodegenerative diseases has been increasingly appreciated in recent years. The aggregation and hyper-phosphorylation of NFs, for instance, has been associated with the progression of some forms of multiple sclerosis and amyotrophic lateral sclerosis. Neurofilaments are incorporated in the structure of intracellular Lewy bodies which are a histopathological hallmark of Parkinson’s Disease.

 

Details of our neurofilament antibodies

 

rat cerebral cortex stained with rabbit polyclonal antibody to Neurofilament

Above: Section of rat cerebral cortex stained with rabbit polyclonal antibody to Neurofilament Medium Cat No R-1395-50 (red).

 

Rat CNS cells stained with mouse monoclonal antibody to alpha-Internexin [1D2]

Above: Mixed cultures of rat CNS cells stained with mouse monoclonal antibody to alpha-Internexin [1D2] Cat No M-1378-250 (red) and Chicken polyclonal antibody to MAP2 Cat No C-1382-50 (green).

 



Neuroscience - ImmunoStar
Immunostar neuroscience

 

Newmarket Scientific is the UK and Ireland Distributor for the ImmunoStar range of antibodies for neuroscience research.

 

This range of primary antibodies was originally provided by DiaSorin (formerly INCSTAR), but in 2001 the range became Immunostar. As these antibodies have been available for many years there are multiple references citing their use.

 

Additionally ImmunoStar antibodies are put through extensive testing before release to ensure both high quality and high titer. This provides excellent reliability and lot-to-lot consistency. These antibodies have also been specifically tested for use in immunohistochemistry. 

 

Details of the full range of 60+ well referenced neuroscience antibodies are available on this link.

 



Why choose Biosensis sheep α-synuclein antibody

The Biosensis sheep anti alpha-synuclein antibody:

 

• Validated for Western blotting (A), Flow cytometry (B), Immunocytochemistry (C) and Immunohistochemistry (D).

• Perfect choice for rodent (and human!) studies: avoid cross-reactivity and background issues that might occur with mouse monoclonals.

• Optimal partner for mouse and rabbit antibodies: maximum flexibility for multi-labelling experiments.

• Long-lasting supply – no need to continuously re-validate for your application: sheep produce up to 30X more serum than rabbits, which reduces inherent polyclonal antibody lot-to-lot variability significantly.

• Choice of anti-sheep and anti-goat secondary antibodies: sheep and goat are very close relatives (use anti-goat antibodies already in your freezer!).

 

Biosensis alpha-synuclein ELISA Kit

 

Related products:

Biosensis alpha-synuclein Rapid ELISA Kit (BEK-2216-1P/2P)

Biosensis chicken alpha-synuclein antibody

Biosensis rabbit anti alpha-synuclein antibody

Phospho-synuclein antibodies