Next Generation Sequencing

NGS Library prep and barcodes for DNA-Seq, RNA-Seq, Small RNA-Seq and more

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Bioo Scientific NEXTflex™ Sequencing Kits & Barcodes have the best ligation efficiency available in a library preparation kit (as shown in these white papers) ensuring you maximise your library diversity and see more of your sample in sequencing.

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Nextflex Rapid Kits (DNA-Seq and RNA-Seq) offer streamlined protocols with minimal hands on time, and have validated automation protocols for PerkinElmer's Sciclone NGS Workstation. Protocols are available for NEXTflex DNA-Seq, RNA-Seq and Metagenomics library prep kits.

Nkt nextflex features apr14



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The NEXTflex Rapid XP DNA-Seq Kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a quick first step in library generation, followed by ligation and PCR (optional for PCR-free workflows). This effective one-tube workflow produces DNA-seq libraries with consistent library size, high yield, low GC-bias, and high coverage.

The kit includes all the required reagents for fragmentation, library prep, and magnetic bead-based cleanup, and it is compatible with your NEXTFLEX® barcodes of choice. This DNA-seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of sample types and sample input amounts from 1 ng to 1 µg to quickly and efficiently generate high-quality libraries for Illumina® sequencing.

Rapid XP DNA-Seq workflow



FEATURED KIT: NEXTflex Rapid DNA-Seq Kit 2.0


The NEXTflex™™ Rapid DNA-Seq Kit 2.0 is designed to prepare multiplexed libraries for single or paired-end sequencing using Illumina® platforms.


This enhanced NEXTflex® kit simplifies workflow by using master mixed reagents and magnetic bead based cleanup, reducing pipetting and eliminating time-consuming steps in library preparation.


Libraries prepared with NEXTflex® rapid DNA-Seq kit 2.0 show higher yield than libraries prepared with Competitor K's kit using the same number of PCR cycles from a 250 ng and 1 ng input .


RAPID DNA-Seq Kit 2.0



FEATURED KIT: NEXTflex Small RNA-Seq Kit v3


The NEXTflex™™ Small RNA-Seq Kit v3 uses patented technology to provide a reduced-bias small RNA library prep for Illumina sequencing with both gel-free and low-input options.


This reduction in bias provides data that better represents true abundances of small RNAs in the starting material and allows more miRNAs to be detected with fewer total reads, increasing efficiency and reducing cost for small RNA sequencing.


Follow the link above for further information. 


Bioo Small RNA Seq


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Illumina Compatible Library Prep Sequencing Kits


Illumina compatible DNA sequencing kits

NEXTflex™ Rapid DNA-Seq 2.0 kits and barcodes

NEXTflex™ Unique Dual Index barcodes

NEXTflex™ Rapid XP DNA-Seq kits and barcodes

NEXTflex™ HT DNA-Seq barcodes (up to 384 barcodes)

NEXTflex™ Rapid DNA-Seq kits and barcodes

NEXTflex™ Rapid DNA-Seq Kit BUNDLE with DNA barcodes

NEXTflex™ ChIP-Seq kits and barcodes

NEXTflex™ PCR-Free DNA-Seq kits and barcodes

NEXTflex™ Cell Free DNA-Seq kit and barcodes

NextPrep-Mag™ cfDNA Isolation Kits

Extract cfDNA from human plasma/serum or urine for cell free DNA-Seq

NEXTflex™ mitochondrial DNA-Seq kit and barcodes



Illumina compatible RNA, qRNA and Small RNA sequencing kits

NEXTflex™ Rapid RNA-Seq kits and barcodes

NEXTflex™ Rapid Directional RNA-Seq kits and barcodes


NEXTflex™ qRNA-Seq kits and barcodes

NEXTflex™ Rapid Directional qRNA-Seq kits and barcodes


NEXTflex™ Rapid Directional mRNA-Seq Kit BUNDLE with barcodes and beads

NEXTflex™ Poly(A) beads for batch purification of mRNA from total RNA.


NEXTflex™ Small-RNA Seq kits and barcodes with randomised adapters


Validated RNA-Seq automation protocols for PerkinElmer's Sciclone Workstation



Illumina compatible Metagenomics sequencing kits

NEXTflex™ 16S V1-V3 Amplicon-Seq kits and barcodes

NEXTflex™ 16S V4 Amplicon-Seq kits and barcodes

NEXTflex™ 16S V5-V6 Amplicon-Seq kits and barcodes

NEXTflex™ 18S ITS Amplicon-Seq kits

Sciclone NGS Workstation Automation Guide for NEXTflex Metagenomics Kits



Illumina compatible Epigenetics sequencing kits

NEXTflex™ Methyl-Seq kits and barcodes

NEXTflex™ Bisulfite Seq kits and barcodes



Illumina compatible disease panel sequencing kits

BRCA1 and BRCA2 amplicon panels (for FFPE and non FFPE samples) 

Cardiovascular Disease amplicon panel

CEBPA amplicon panel

Colon-1 amplicon Panel

Colorectal Cancer-2 amplicon Panel

Congenital Adrenal Hyperplasia amplicon Panel

Congenital Hyperinsulinism amplicon Panel

Cystic Fibrosis amplicon panel

Diabetes-3 amplicon panel

Diabetes-4 amplicon panel

DMD (Duchenne Muscular Dystrophy) amplicon panel

Epilepsy-2 amplicon panel

HBOC-3 amplicon panel

Marfan's Disease amplicon panel

Mediterranean Fever amplicon panel (for fresh or frozen samples) 

MODY-1 amplicon panel

MODY-2 amplicon Panel

Nephrotic Syndrome-1 amplicon panel

Neurofibromatosis amplicon panel

Obesity-1 amplicon Panel

Obesity-2 amplicon Panel

Periodic Fever-1 amplicon panel

Periodic Fever-2 amplicon panel

Phenylketonuria amplicon panel

TP53 amplicon panel

TP53 amplicon Panel for FFPE



Illumina compatible Target Capture sequencing kits

NEXTflex™ Rapid Pre-Capture Combo kit (NimbleGen SeqCap EZ Library Compatible)


NEXTflex™ Pre- and Post- Capture Combo kit (Agilent SureSelect XT and XT2 compatible)


NEXTflex™ DNA barcodes and barcode blockers 



Ion Torrent-Based Compatible Sequencing Kits


Ion Torrent compatible DNA sequencing kits

NEXTflex™ DNA-Seq Kits and Barcodes for Ion Systems

NEXTflex™ Cell Free DNA-Seq Kits and Barcodes for Ion System


Ion Torrent compatible Amplicon Panel sequencing kits

Ion compatible BRCA1 and BRCA2 Amplicon Panels (for FFPE and non FFPE Samples)



NGS Blog and White Papers


For NGS tech tips, news and exciting developments visit the Bioo Scientific NGS Blog:

The "What's Next in NGS?" Blog


And read our application notes and white papers:


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Tools for Enhancing Sequence Diversity and Reducing Bias in DNA-seq Next Generation Sequencing (NGS) Library Preparation

Achieving High Coverage and Yield from GC and AT Rich Genomes in NGS Library Preparation

Comparison of Yield and Size Distribution of cfDNA Extracted from Human Plasma


Small RNA:

Randomized Adapters for Reducing Bias in Small RNA-Seq Libraries 

RNA Extraction and Small RNA Library Production for Next Generation Sequencing (NGS)

Increasing Ligation Efficiency and Discovery of miRNAs for Small RNA NGS Sequencing Library Prep with Plant Samples



Quantitative, Directional and Standard RNA-Seq

Nature Methods Application Note: "Molecular indexing for improved RNA-Seq analysis in NGS"

Directional qRNA-Seq Combining the Power of Stranded RNA-Seq with the Quantitative Precision of Molecular Labels



16S rRNA Amplicon Sequencing Offers Enhanced Metagenomic Detection