Newmarket Scientific
Newmarket Scientific

Next Generation Sequencing

NGS Library prep and barcodes for DNA-Seq, RNA-Seq, Small RNA-Seq and more

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NEXTflex® Library Prep Kits & Barcodes for NGS


DNA (DNA/ChiP), RNA (quantitative/directional), Small RNA, Metagenomics (16s/18s), Epigenetics (Methyl/Bisulfite) and multiple barcode options (HT barcodes/Unique Dual Indexes/up to 384 barcodes).


Robust yields • High quality data (Coverage » GC bias » Mapping rate » Mitigation of duplication rate) • PCR-free compatible workflow • Low input • Automation compatible


Streamlined protocols with minimal hands on time and validated protocols for the Sciclone Workstation. Automated protocols are available for DNA-Seq, RNA-Seq and Metagenomics library prep kits.



Rapid DNA 2.0 or Rapid XP - Two Fragmentation Options


Mechanical fragmentation

The NEXTflex® Rapid DNA-Seq Kit 2.0 is designed to prepare multiplexed libraries with high yield for single or paired-end sequencing using Illumina® platforms.


This enhanced kit simplifies workflow with master mix reagents and magnetic bead based cleanup, reducing pipetting and eliminating time-consuming steps in library prep.


Libraries prepared with NEXTflex® rapid DNA-Seq kit 2.0 show higher yield than libraries prepared with Competitor K's kit using the same number of PCR cycles with 250 ng or 1 ng input.


RAPID DNA-Seq Kit 2.0


Enzymatic fragmentation

The NEXTflex® Rapid XP DNA-Seq Kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a quick first step in library generation, followed by ligation and PCR (optional for PCR-free workflows).


This effective one-tube workflow produces DNA-seq libraries with consistent library size, high yield, low GC-bias, and high coverage. The kit includes all the required reagents for fragmentation, library prep, and magnetic bead-based cleanup, and it is compatible with all NEXTFLEX barcode options.


This DNA-seq library prep kit is highly flexible in terms of sample type and input amount from 1 ng to 1 µg to quickly and efficiently generate high-quality libraries for Illumina® sequencing.


Rapid-XP DNA Kit Work Flow



NEXTflex Small RNA-Seq Kit v3


The NEXTflex® Small RNA-Seq Kit v3 uses patented technology to provide a reduced-bias small RNA library prep for Illumina sequencing with both gel-free and low-input options.


This reduction in bias provides data that better represents the true abundances of the small RNAs in your samples and allows more miRNAs to be detected with fewer total reads, increasing efficiency and reducing the overall cost of your small RNA sequencing.


Bioo Small RNA Seq


Illumina Compatible Library Prep Sequencing Kits


DNA Sequencing Kits


NEXTFLEX® Rapid DNA-Seq 2.0 kits and barcodes

NEXTFLEX® Rapid XP DNA-Seq kits and barcodes


NEXTFLEX® Unique Dual Index barcodes

NEXTFLEX® HT DNA-Seq barcodes (up to 384 barcodes)


NEXTFLEX® ChIP-Seq kits and barcodes

NEXTFLEX® PCR-Free DNA-Seq kits and barcodes


NEXTFLEX® Cell Free DNA-Seq kit and barcodes

NextPrep-Mag™ cfDNA Isolation Kits

Extract cfDNA from human plasma/serum or urine for cell free DNA-Seq


NEXTFLEX® mitochondrial DNA-Seq kit and barcodes



RNA, qRNA and Small RNA Sequencing Kits


NEXTFLEX® Small-RNA Seq kit v3.0 with barcodes - See more of your sample!


NEXTFLEX® Rapid Directional RNA-Seq kit 2.0 and barcodes

NEXTFLEX® Rapid Directional qRNA-Seq kits and barcodes


NEXTFLEX® Combo-Seq mRNA/miRNA Kit


NEXTFLEX® Poly(A) beads 2.0 for batch purification of mRNA from total RNA.



Validated RNA-Seq automation protocols for PerkinElmer's Sciclone Workstation



Metagenomics Sequencing Kits


NEXTFLEX® 16S V1-V3 Amplicon-Seq kits and barcodes

NEXTFLEX® 16S V4 Amplicon-Seq kits and barcodes

NEXTFLEX® 16S V5-V6 Amplicon-Seq kits and barcodes

NEXTFLEX® 18S ITS Amplicon-Seq kit


Sciclone NGS Workstation Automation Guide for NEXTflex Metagenomics Kits



Epigenetics Sequencing Kits


NEXTFLEX® Methyl-Seq kits and barcodes

NEXTFLEX® Bisulfite Seq kits and barcodes



Target Capture Sequencing Kits


NEXTFLEX® Rapid Pre-Capture Combo kit (NimbleGen SeqCap EZ Library Compatible)

NEXTFLEX® Pre- and Post- Capture Combo kit (SureSelect XT and XT2 compatible)

NEXTFLEX® DNA barcodes and barcode blockers