Newmarket Scientific
Newmarket Scientific

Next Generation Sequencing

NGS Library prep and barcodes for DNA-Seq, RNA-Seq, Small RNA-Seq and more

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NEXTflex® Library Prep Kits & Barcodes for NGS


DNA (DNA/ChiP), RNA (quantitative/directional), Small RNA, Metagenomics (16s/18s), Epigenetics (Methyl/Bisulfite) and multiple barcode options (HT barcodes/Unique Dual Indexes/up to 384 barcodes).


Robust yields • High quality data (Coverage » GC bias » Mapping rate » Mitigation of duplication rate) • PCR-free compatible workflow • Low input • Automation compatible


Streamlined protocols with minimal hands on time and validated protocols for the Sciclone Workstation. Automated protocols are available for DNA-Seq, RNA-Seq and Metagenomics library prep kits.



NEW NEXTFLEX® Universal Blockers for Targeted Sequencing Applications


During target enrichment, adapters can interact with the complementary adapter sequence strand of another library molecule leading to off target reads. NEXTFLEX® Universal Blockers have been developed to solve that problem and improve on-target capture.


Their design is effective in blocking single-index or dual-index libraries, without being affected by index length or the presence of a unique molecular index (UMI). They are also compatible with ligation-based and tagmentation-based workflows.


• Simplify your workflows with just one set of blockers for all your applications

• Ready-to-use formulation compatible with any baits for hybridisation enrichment

• High performance regardless of index length and panel size

• Very significant cost saving



Rapid DNA 2.0 or Rapid XP - Two Fragmentation Options


Mechanical fragmentation

The NEXTflex® Rapid DNA-Seq Kit 2.0 is designed to prepare multiplexed libraries with high yield for single or paired-end sequencing using Illumina® platforms.


This enhanced kit simplifies workflow with master mix reagents and magnetic bead based cleanup, reducing pipetting and eliminating time-consuming steps in library prep.


Libraries prepared with NEXTflex® rapid DNA-Seq kit 2.0 show higher yield than libraries prepared with Competitor K's kit using the same number of PCR cycles with 250 ng or 1 ng input.


RAPID DNA-Seq Kit 2.0


Enzymatic fragmentation

The NEXTflex® Rapid XP DNA-Seq Kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a quick first step in library generation, followed by ligation and PCR (optional for PCR-free workflows).


This effective one-tube workflow produces DNA-seq libraries with consistent library size, high yield, low GC-bias, and high coverage. The kit includes all the required reagents for fragmentation, library prep, and magnetic bead-based cleanup, and it is compatible with all NEXTFLEX barcode options.


This DNA-seq library prep kit is highly flexible in terms of sample type and input amount from 1 ng to 1 µg to quickly and efficiently generate high-quality libraries for Illumina® sequencing.


Rapid-XP DNA Kit Work Flow



NEXTflex Small RNA-Seq Kit v3


The NEXTflex® Small RNA-Seq Kit v3 uses patented technology to provide a reduced-bias small RNA library prep for Illumina sequencing with both gel-free and low-input options.


This reduction in bias provides data that better represents the true abundances of the small RNAs in your samples and allows more miRNAs to be detected with fewer total reads, increasing efficiency and reducing the overall cost of your small RNA sequencing.


Bioo Small RNA Seq