Newmarket Scientific
Newmarket Scientific








Highlighted Antibody - Melan A Clone BS52



Melan A (MART-1) is a transmembrane protein which is expressed in skin melanocytes and melanocyte lineages, and is ecognised by autologous cytotoxic T lymphocytes.

 

This antibody is useful for the identification of melanomas and it should be included in standard melanoma diagnostic panels. It does not cross react with cells of adrenal cortex.

 

This antibody provides truly optimal staining and as with all our antibodies we provide a 100% guarantee, so trying it really is risk free.

 

Suggested Protocol after paraffin removal and rehydration:

 

1. Pretreatment: HIER pH9

2. Wash (TBS-Tween)

3. Primary antibody: Melan A 1:100 – 1:400, 30 min.

4. Wash

5. 3% H2O2, 10 min 

6. Wash

7. HRP One-Step Polymer, 30 min

8. Wash

9. DAB high contrast Kit, 10 min

10. Aqua

11. CuSO4 -post enhancement, 5 min

12. Aqua

13. Counter staining in diluted Mayer, 1 min

14. Bluing, 7 min in tap water

15. Dehydration, clearing and mounting

 

Optibodies™ are mouse and rabbit monoclonal antibodies that have been developed specifically for use in immunohistochemistry to have high affinity and high specificity to their target antigens, give a high signal to noise ratio and can be used to achieve an intensive and specific staining.

 

Anti-Melan A (Clone BS52)  Anti-Melan-Datasheet

 

Please visit our optibodies page and follow the individual product links for more information about the full optibodies range.

 


 

Optimal results for Optibodies™ in External QC assessments!

 

In recent quality assessments by External QC service providers, optimal staining results were obtained for SEVEN different Optibodies clones:

SOX10 - Transcription factor SOX-10 (Clone BS7)

CK5 - Cytokeratin 5 (Clone BSR55)

HER2 - IHC (Clone BSR44)

EpCAM - Epithelial cell-cell adhesion molecule (Clone BS14)

SMA - Alpha-Smooth Muscle Actin (Clone BS66)

MLA - Melan A (Clone BS52)

PDL-1 - Programmed Death Ligand 1 (Clone BSR90)

 



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