Newmarket Scientific
Newmarket Scientific








NEWS: The role of retromer component VPS-35 in neurodegenerative diseases



Endosomes are important protein sorting stations contributing to cell homeostasis. Cargoes, originating from the plasma membrane or the biosynthesis pathways and entering the endosomal system, are either retained in endosomes for subsequent lysosomal degradation or exported from the endosomes for reuse or recycling. Deficiencies in the endosomal sorting system have been linked to neurodegenerative diseases such as Alzheimer’s and Parkinson’s.

 

Retromer is an evolutionarily conserved protein essential for the transport of transmembrane proteins from the endosomes back to the trans-Golgi network or to the plasma membrane via the recycling pathway. Retromer is a pentameric complex composed of a cargo-selective trimeric complex, with the major subunit being VPS-35 (vacuolar protein sorting 35) which is combined with a sorting nexin dimer.

 

The endosomal retromer is known to bind and recruit the WASH complex (Wiskott–Aldrich syndrome and SCAR homolog) to the endosomal membranes helping the formation of actin patches that facilitate protein sorting. It is thought that mutations in the VPS-35 gene weaken the binding of VPS-35 with an element of the WASH complex, which results in abnormal protein sorting. The D620N mutation in VPS-35 has been associated with Parkinson’s disease. Below we detail the NS reagents VPS-35 antibodies developed to aid research.in this area.

 


NS Reagents VPS-35 antibodies:

NS Reagents Anti-VPS35 antibody AB19-10109

Applications: WB | IHC Reactivity: Hu | Ms

 

NS Reagents Anti-VPS35 antibody AB19-10110 (N Terminal)

Applications: WB Reactivity: Hu | Ms | Rt

 

Complete the form below to request a free sample of either antobody.

 

 

References:

Parkinson’s disease-linked D620N VPS35 knockin mice manifest tau neuropathology and dopaminergic neurodegeneration, Chen X et al, Proc Natl Acad Sci U S A. 2019 Mar 19; 116(12): 5765–5774. 


The functional roles of retromer in Parkinson's disease, Cui Y et al, FEBS Lett. (2018) 


The retromer complex – endosomal protein recycling and beyond Seaman M.N. J, J Cell Sci. 2012 Oct 15; 125(20): 4693–4702. doi: 10.1242/jcs.103440 


Retromer: A Master Conductor of Endosome Sorting, Burd C et al, Cold Spring Harb Perspect Biol. 2014 Feb; 6(2): a016774. doi: 10.1101/cshperspect.a016774 


Retromer-mediated endosomal protein sorting: all WASHed up! Seaman MNJ et al, Trends Cell Biol. 2013 Nov;23(11):522-8. doi: 10.1016/j.tcb.2013.04.010. Epub 2013 May 28. 

 



Complete this form to request your free sample (please state which antibody you would like).