Newmarket Scientific
Newmarket Scientific








Staining Myelin with the Biosensis Black Gold II RTD kit and Myelin Basic Protein Antibodies



Myelin sheath is found in the white matter of the brain and is vital to the normal functioning of the nervous system. Its main function is to insulate and protect the axon of neurons whilst contributing to the rapid and efficient transmission of signals through the nerve cells. Demyelination (damage or loss of myelin due to injuries such as trauma, genetic defects or autoimmune reactions) is the cause of several CNS pathologies such as multiple sclerosis.

 

Biosensis provides several tools to localise myelin including a staining kit using a gold based dye, Black Gold II (TR-100-BG) and a range of quality myelin basic protein antibodies from different hosts and suitable for a range of applications including immunohistochemistry, (IHC).

 

The Biosensis Black Gold II ready-to-dilute kit (TR-100-BG) has been specifically designed to identify normal and pathogenic myelin (both individual fibres and tracts), along with the option of co-localising cell bodies via the Toluidine Blue O counter stain. In particular, the Black Gold II dye has a higher contrast, is far safer than heavy metal dyes such as osmium tetroxide, penetrates better and provides better results than earlier stains.

 

The Biosensis Black Gold II RTD myelin staining kit is in our opinion simply the best kit available on the market due to its ease of use, great results and low price per use.

 

The Biosensis Black Gold II kit can make 100mL of the Black Gold II solution,

twice as much stain as other kits on the market

 

AND it contains a counter stain

 

AND there is also no need to dissolve the dye as all the components are ready to dilute.

 

 

Left: Analysis of MBP expression in rat brain cerebellum in Immunohistochemistry with chicken anti-MBP antibody C-1383-50 (1:5,000, green). Section was co-stained with rabbit antibody to NF-L (R-1392-50, 1:5,000, red). The MBP antibody stains oligodendrocytes and myelin sheathes around axons, while the NF-L antibody labels dendrites and axons of neuronal cells.

 

Right: Bright field illumination (60X mag) of the dentate gyrus of a normal mouse reveals individual Black-Gold II stained myelinated fibres and Toluadine Blue O stained polymorph cells (centre) and granule cells (top). Photo courtesy of Dr. Larry Schmued.

 

Related products:

 

Myelin Basic Protein (MBP) antibodies 

 

CNPase antibodies