Newmarket Scientific
Newmarket Scientific

Staining Myelin with the Biosensis Black Gold II RTD kit and Myelin Basic Protein Antibodies

Myelin sheath is found in the white matter of the brain and is vital to the normal functioning of the nervous system. Its main function is to insulate and protect the axon of neurons whilst contributing to the rapid and efficient transmission of signals through the nerve cells. Demyelination (damage or loss of myelin due to injuries such as trauma, genetic defects or autoimmune reactions) is the cause of several CNS pathologies such as multiple sclerosis.


Biosensis provides several tools to localise myelin including a myelin staining kit using a gold based dye, Black Gold II (TR-100-BG) and a range of quality myelin basic protein antibodies from different hosts and suitable for a range of applications including immunohistochemistry, (IHC).


Further information about these reagents can be found below, but should you have any questions about these reagents or if you require a quotation, please contact



Biosensis Black Gold II RTD myelin staining kit

The myelin staining kit from Biosensis, Black Gold II ready-to-dilute kit (TR-100-BG) has been specifically designed to identify normal and pathogenic myelin (both individual fibres and tracts), along with the option of co-localising cell bodies via the Toluidine Blue O counter stain. In particular, the Black Gold II dye has a higher contrast, is far safer than heavy metal dyes such as osmium tetroxide, penetrates better and provides better results than earlier stains.


The Biosensis Black Gold II RTD myelin staining kit is in our opinion simply the best kit available on the market due to its ease of use, great results and low price per use.


The Biosensis Black Gold II kit can make 100mL of the Black Gold II solution,

twice as much stain as other kits on the market


AND it contains a counter stain


AND there is also no need to dissolve the dye as all the components are ready to dilute.



Bright field illumination (60X mag) of the dentate gyrus of a normal mouse reveals individual Black-Gold II stained myelinated fibres and Toluidine Blue O stained polymorph cells (centre) and granule cells (top). Photo courtesy of Dr. Larry Schmued.


Myelin basic protein antibodies

Myelin basic protein (MBP) plays a crucial role in the formation of neuronal myelin sheaths of oligodendrocytes and Schwann cells. It is used as a biomarker of demyelination. Recent studies have used anti-MBP antibodies for the diagnosis and prognosis of patients with brain injury or neurodegenerative diseases.


Biosensis offers a range of MBP antibodies in different hosts suitable for immunocytochemistry (ICC), immunohistochemistry (IHC) and western blotting (WB) applications:

Myelin basic protein (MBP) Antibody, Mouse monoclonal (M-1384-50)

Myelin basic protein (MBP) Antibody, Chicken polyclonal (C-1383-50)

Myelin basic protein (MBP) Antibody, Sheep polyclonal (S-023-100)

Myelin basic protein (MBP) Antibody, Rabbit Polyclonal (R-107-100)


The MBP antibodies from Biosensis are easy to use, reliable and more specific. They are preferred in many neuronal experiments for:

• identifying oligodendrocytes and Schwann cells in neural cell culture

• observing myelin sheaths and myelinated cells in sectioned materials

• exploring MBP gene products in Western Blotting


For any further information about our myelin basic protein antibodies, please email




A. Analysis of MBP expression in rat brain cerebellum in Immunohistochemistry with chicken anti-MBP antibody C-1383-50 (1:5,000, green). Section was co-stained with rabbit antibody to NF-L (R-1392-50, 1:5,000, red). The MBP antibody stains oligodendrocytes and myelin sheaths around axons, while the NF-L antibody labels dendrites and axons of neuronal cells.


B. Staining of rat hippocampal section with mouse antibody to myelin basic protein M-1384-50 (MBP, 1:5,000, green) by Immunohistochemistry. NF-H was co-stained with R-1389-50 (1:2,000, red). The MBP antibody stains oligodendrocyte cell bodies and the myelin sheaths around axons, while the NF-H antibody labels the axons themselves.


Related products:


Myelin Basic Protein (MBP) antibodies 


CNPase antibodies