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ImmunoStar 24504 100 uL £399.00
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Product Detail: 5-HT (Serotonin) 1A Receptor Antibody
Background Info: Raised against synthetic peptide sequence corresponding to amino acids 294-312 of the rat 5-HT1A receptor.
Product Type: Antibody - Antibodies
Antibody Type: polyclonal
Format: Liquid
Host Animal: Rabbit
Species Reactivity: Guinea Pig, Human, Monkey, Mouse, Rat
Immunogen: Rat 5-HT1A receptor (294-312)
Applications: Immunohistochemistry, Immunocytochemistry, immunofluorescence, Western Blot
Data Sheet: PLEASE CLICK HERE TO DOWNLOAD
Additional Info: The 5-HT 1A Receptor Antibody was raised against synthetic peptide sequence corresponding to amino acids 294-312 of the rat 5-HT1A receptor. The antiserum is provided as 100 µL of affinity purified serum containing 1% BSA. The antiserum demonstrates strongly positive labeling of rat cortex, arcuate and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/100 - 1/200 in PBS - Bn/Av-HRP detection. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunohistochemistry and Western blot. Western blot showed a single band of approximately 45 kD. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT1A receptors.
PubMed IDs: 23518679, 20510890, 19423080, 18537141, 25253632
Product category: Cell Cycle,