The NEXTflex PCR-free DNA Sequencing Kits contain the reagents to prepare 8 or 48 genomic DNA samples for Illumina-compatible next-generation sequencing. The shelf life of all reagents is 12 months when stored properly at -20°C. Ships on dry ice.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Amplifying AT or GC rich genomic regions often leads to sequence biased nucleotide compositions and poses a serious challenge during analysis. Using specially designed master-mixed enzymes, the NEXTflex PCR-Free DNA Sequencing kit completely eliminates the need for amplification, enabling better read mapping and a reduction in duplicate sequences leading to reduced sequence cost and bias for more representative base identities and better de novo assembly.
In addition, the NEXTflex PCR-Free DNA Sequencing Kit features patent pending Enhanced Adapter Ligation Technology resulting in library preps with a larger number of unique sequencing reads. By making improvements to the ligation enzymatic mix, the user will now have the ability to perform ligations with longer adapters and expect to see better binding efficiencies. The NEXTflex PCR-Free Sequencing Kit simplifies workflow by using master mixed reagents and magnetic bead based cleanup, reducing pipetting and eliminating time consuming steps in library preparation. In addition, the availability of up to 48 unique adapter barcodes with the NEXTflex PCR-Free Barcode Kits makes this the most high-throughput kit available
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] >>> Eliminates amplification bias and poor sequence representation >>> Improves read mapping >>> Reduces duplicate sequences >>> Better de novo assembly >>> Significantly faster library preparation time >>> Enhanced adapter ligation technology with NEXTflex Ligation >>> Flexible barcode options - kits contain 6, 12, 24 or 48 unique barcodes >>> Cost-effective library prep solution >>> Bead-based cleanup protocol >>> Automation-friendly workflow is compatible with liquid handlers >>> Barcoded adapters for multiplexing contain embedded index sequence
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5142-01
The NEXTflex PCR-free DNA Sequencing Kits contain the reagents to prepare 8 or 48 genomic DNA samples for Illumina-compatible next-generation sequencing. The shelf life of all reagents is 12 months when stored properly at -20°C. Ships on dry ice.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Amplifying AT or GC rich genomic regions often leads to sequence biased nucleotide compositions and poses a serious challenge during analysis. Using specially designed master-mixed enzymes, the NEXTflex PCR-Free DNA Sequencing kit completely eliminates the need for amplification, enabling better read mapping and a reduction in duplicate sequences leading to reduced sequence cost and bias for more representative base identities and better de novo assembly.
In addition, the NEXTflex DNA Sequencing Kit features patent pending Enhanced Adapter Ligation Technology resulting in library preps with a larger number of unique sequencing reads. By making improvements to the ligation enzymatic mix, the user will now have the ability to perform ligations with longer adapters and expect to see better binding efficiencies. The NEXTflex kit simplifies workflow by using master mixed reagents and magnetic bead based cleanup, reducing pipetting and eliminating time consuming steps in library preparation. In addition, the availability of up to 48 unique adapter barcodes makes this the most high-throughput kit available.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] >>> Eliminates amplification bias and poor sequence representation >>> Improves read mapping >>> Reduces duplicate sequences >>> Better de novo assembly >>> Significantly faster library preparation time >>> Enhanced adapter ligation technology with NEXTflex Ligation >>> Flexible barcode options - kits contain 6, 12, 24 or 48 unique barcodes >>> Cost-effective library prep solution >>> Bead-based cleanup protocol >>> Automation-friendly workflow is compatible with liquid handlers >>> Barcoded adapters for multiplexing contain embedded index sequence
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5142-02
The NEXTflex DNA Barcodes Kits contains 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTflex DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits and PCR-free sequencing workflows.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 6 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Up to 48 multiplexed samples (up to 384 reactions per kit) >>> Compatible with PCR-free sequencing workflows >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514110
The NEXTflex DNA Barcodes Kits contains 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTflex DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits and PCR-free sequencing workflows.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 6 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Up to 48 multiplexed samples (up to 384 reactions per kit) >>> Compatible with PCR-free sequencing workflows >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514112
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