NEXTFLEX® UDI Barcoded Adapters (1-1,536) for Ultra High-Throughput Multiplexing
Sets of 384 UDI barcodes for multiplexing NGS libraries compatible with Illumina® Up to 1,536 (10-nt) adapters available for purchase off-the-shelf Every lot is functionally validated and tested for index purity by sequencing
Highest Sample Multiplexing Options for Illumina® Sequencing The superior design of the NEXTFLEX® Unique Dual Index barcodes allows a broad range of multiplexing users can multiplex anywhere between two samples and 1,536 samples in a single sequencing run. The wide range of multiplexing options enables barcode rotation (avoiding frequent use of the same barcode set) to mitigate run-to-run sample carryover and crosstalk. NEXTFLEX® Unique Dual Index barcodes are compatible with NEXTFLEX® library prep kits designed for Illumina® paired-end and single-read sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
Highest Level of Data Integrity The NEXTFLEX® Unique Dual Index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction. Each NEXTFLEX® Unique Dual Index barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices improves data integrity by correctly removing mis-assigned reads. Every lot is functionally validated and tested for index purity by sequencing.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix** (50 µM)
The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc).
* These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
** The Primer Mix 2.0 is only intended for use with the NEXTFLEX® Rapid XP DNA-Seq kit and the NEXTFLEX® Rapid DNA-Seq kit 2.0; if the primer mix is to be used with any other library kits of choice, additional dilutions may be required. For additional guidance, please contact us.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - Illumina Compatible
Search Terms:
Rapid DNA, Cell Free DNA-Seq, Dual Index Barcodes, NEXTflex Unique Dual Index Barcoded Adapters 1536 UDI Set
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
NEXTFLEX® UDI Barcoded Adapters (1-1,536) for Ultra High-Throughput Multiplexing
Sets of 384 UDI barcodes for multiplexing NGS libraries compatible with Illumina® Up to 1,536 (10-nt) adapters available for purchase off-the-shelf Every lot is functionally validated and tested for index purity by sequencing
Highest Sample Multiplexing Options for Illumina® Sequencing The superior design of the NEXTFLEX® Unique Dual Index barcodes allows a broad range of multiplexing users can multiplex anywhere between two samples and 1,536 samples in a single sequencing run. The wide range of multiplexing options enables barcode rotation (avoiding frequent use of the same barcode set) to mitigate run-to-run sample carryover and crosstalk. NEXTFLEX® Unique Dual Index barcodes are compatible with NEXTFLEX® library prep kits designed for Illumina® paired-end and single-read sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
Highest Level of Data Integrity The NEXTFLEX® Unique Dual Index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction. Each NEXTFLEX® Unique Dual Index barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices improves data integrity by correctly removing mis-assigned reads. Every lot is functionally validated and tested for index purity by sequencing.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix** (50 µM)
The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc).
* These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
** The Primer Mix 2.0 is only intended for use with the NEXTFLEX® Rapid XP DNA-Seq kit and the NEXTFLEX® Rapid DNA-Seq kit 2.0; if the primer mix is to be used with any other library kits of choice, additional dilutions may be required. For additional guidance, please contact us.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - Illumina Compatible
Search Terms:
Rapid DNA, Cell Free DNA-Seq, Dual Index Barcodes, NEXTflex Unique Dual Index Barcoded Adapters 1536 UDI Set
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
NEXTFLEX® UDI Barcoded Adapters (1-1,536) for Ultra High-Throughput Multiplexing
Sets of 384 UDI barcodes for multiplexing NGS libraries compatible with Illumina® Up to 1,536 (10-nt) adapters available for purchase off-the-shelf Every lot is functionally validated and tested for index purity by sequencing
Highest Sample Multiplexing Options for Illumina® Sequencing The superior design of the NEXTFLEX® Unique Dual Index barcodes allows a broad range of multiplexing users can multiplex anywhere between two samples and 1,536 samples in a single sequencing run. The wide range of multiplexing options enables barcode rotation (avoiding frequent use of the same barcode set) to mitigate run-to-run sample carryover and crosstalk. NEXTFLEX® Unique Dual Index barcodes are compatible with NEXTFLEX® library prep kits designed for Illumina® paired-end and single-read sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
Highest Level of Data Integrity The NEXTFLEX® Unique Dual Index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction. Each NEXTFLEX® Unique Dual Index barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices improves data integrity by correctly removing mis-assigned reads. Every lot is functionally validated and tested for index purity by sequencing.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix** (50 µM)
The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc).
* These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
** The Primer Mix 2.0 is only intended for use with the NEXTFLEX® Rapid XP DNA-Seq kit and the NEXTFLEX® Rapid DNA-Seq kit 2.0; if the primer mix is to be used with any other library kits of choice, additional dilutions may be required. For additional guidance, please contact us.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - Illumina Compatible
Search Terms:
Rapid DNA, Cell Free DNA-Seq, Dual Index Barcodes, NEXTflex Unique Dual Index Barcoded Adapters 1536 UDI Set
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
NEXTFLEX® UDI Barcoded Adapters (1-1,536) for Ultra High-Throughput Multiplexing
Sets of 384 UDI barcodes for multiplexing NGS libraries compatible with Illumina® Up to 1,536 (10-nt) adapters available for purchase off-the-shelf Every lot is functionally validated and tested for index purity by sequencing
Highest Sample Multiplexing Options for Illumina® Sequencing The superior design of the NEXTFLEX® Unique Dual Index barcodes allows a broad range of multiplexing users can multiplex anywhere between two samples and 1,536 samples in a single sequencing run. The wide range of multiplexing options enables barcode rotation (avoiding frequent use of the same barcode set) to mitigate run-to-run sample carryover and crosstalk. NEXTFLEX® Unique Dual Index barcodes are compatible with NEXTFLEX® library prep kits designed for Illumina® paired-end and single-read sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
Highest Level of Data Integrity The NEXTFLEX® Unique Dual Index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction. Each NEXTFLEX® Unique Dual Index barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices improves data integrity by correctly removing mis-assigned reads. Every lot is functionally validated and tested for index purity by sequencing.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix** (50 µM)
The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc).
* These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
** The Primer Mix 2.0 is only intended for use with the NEXTFLEX® Rapid XP DNA-Seq kit and the NEXTFLEX® Rapid DNA-Seq kit 2.0; if the primer mix is to be used with any other library kits of choice, additional dilutions may be required. For additional guidance, please contact us.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - Illumina Compatible
Search Terms:
Rapid DNA, Cell Free DNA-Seq, Dual Index Barcodes, NEXTflex Unique Dual Index Barcoded Adapters 1536 UDI Set
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
NEXTFLEX® UDI Barcoded Adapters (1-1,536) for Ultra High-Throughput Multiplexing
Sets of 384 UDI barcodes for multiplexing NGS libraries compatible with Illumina® Up to 1,536 (10-nt) adapters available for purchase off-the-shelf Every lot is functionally validated and tested for index purity by sequencing
Highest Sample Multiplexing Options for Illumina® Sequencing The superior design of the NEXTFLEX® Unique Dual Index barcodes allows a broad range of multiplexing users can multiplex anywhere between two samples and 1,536 samples in a single sequencing run. The wide range of multiplexing options enables barcode rotation (avoiding frequent use of the same barcode set) to mitigate run-to-run sample carryover and crosstalk. NEXTFLEX® Unique Dual Index barcodes are compatible with NEXTFLEX® library prep kits designed for Illumina® paired-end and single-read sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
Highest Level of Data Integrity The NEXTFLEX® Unique Dual Index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction. Each NEXTFLEX® Unique Dual Index barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices improves data integrity by correctly removing mis-assigned reads. Every lot is functionally validated and tested for index purity by sequencing.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix** (50 µM)
The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc).
* These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
** The Primer Mix 2.0 is only intended for use with the NEXTFLEX® Rapid XP DNA-Seq kit and the NEXTFLEX® Rapid DNA-Seq kit 2.0; if the primer mix is to be used with any other library kits of choice, additional dilutions may be required. For additional guidance, please contact us.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - Illumina Compatible
Search Terms:
Rapid DNA, Cell Free DNA-Seq, Dual Index Barcodes, NEXTflex Unique Dual Index Barcoded Adapters 1536 UDI Set
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The NEXTflex rapid DNA-seq 2.0 kit is designed to prepare multiplexed libraries for single or paired-end sequencing using Illumina platforms. The enhanced NEXTflex kit simplifies workflow by using master mixed reagents and magnetic bead based cleanup, reducing pipetting and eliminating time-consuming steps in library preparation. The NEXTflex rapid DNA-seq kit produces library preps with a larger number of unique sequencing reads, allowing for robust genome coverage in challenging GC-rich sequences that can introduce bias.
The NEXTflex rapid DNA-seq 2.0 kit bundle offers all the advantages and reagents of the core kit, but with the additional benefit of including unique NEXTflex-HT barcodes for the number of reactions (8, 48, or 96 reaction size kits) in a single-use format. This allows the lab to tie all the necessary components of the library prep workflow to a single lot for ease of purchasing and tracking of consumables.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Complete library prep solution, including size selection beads Bundle kits available with library prep reagents and barcodes Up to 1536 barcodes available upon request Convenient and cost-effective package in a 8, 48, or 96 reaction format Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Kits containing up to 96 unique barcodes Compatible with Illumina HiSeq, MiSeq and GAIIx platforms Prepare single, paired-end and multiplexed genomic DNA libraries Bulk pricing available to meet research goals
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - Illumina Compatible
Applications:
Rapid DNA
Search Terms:
Rapid DNA
Additional Info:
Flexible Multiplexing Options
The NEXTflex Adapters are long, annealed adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. These barcodes can be used with single, paired-end and multiplex reads. Bioo Scientific strives to make available flexible barcoding options. The NEXTflex Rapid DNA-Seq Kit is designed to be used with the NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes and NEXTflex-HT Barcodes when 10 ng or more of starting material is used. These barcodes are available in sets of 6, 12, 24, 48, 96 and 384 unique indexed adapters.
When less than 10 ng of starting material is available, the NEXTflex ChIP-Seq Barcodes and NEXTflex-96 ChIP-Seq Barcodes should be used. These barcodes are available in sets of 6, 12, 24, 48, and 96 unique indexed adapters.
The NEXTflex Rapid DNA-Seq Kit contains enough material to prepare 8 or 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The NEXTflex rapid DNA-seq 2.0 kit is designed to prepare multiplexed libraries for single or paired-end sequencing using Illumina platforms. The enhanced NEXTflex kit simplifies workflow by using master mixed reagents and magnetic bead based cleanup, reducing pipetting and eliminating time-consuming steps in library preparation. The NEXTflex rapid DNA-seq kit produces library preps with a larger number of unique sequencing reads, allowing for robust genome coverage in challenging GC-rich sequences that can introduce bias.
The NEXTflex rapid DNA-seq 2.0 kit bundle offers all the advantages and reagents of the core kit, but with the additional benefit of including unique NEXTflex-HT barcodes for the number of reactions (8, 48, or 96 reaction size kits) in a single-use format. This allows the lab to tie all the necessary components of the library prep workflow to a single lot for ease of purchasing and tracking of consumables.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Complete library prep solution, including size selection beads Bundle kits available with library prep reagents and barcodes Up to 1536 barcodes available upon request Convenient and cost-effective package in a 8, 48, or 96 reaction format Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Kits containing up to 96 unique barcodes Compatible with Illumina HiSeq, MiSeq and GAIIx platforms Prepare single, paired-end and multiplexed genomic DNA libraries Bulk pricing available to meet research goals
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - Illumina Compatible
Applications:
Rapid DNA
Search Terms:
Rapid DNA
Additional Info:
Flexible Multiplexing Options
The NEXTflex Adapters are long, annealed adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. These barcodes can be used with single, paired-end and multiplex reads. Bioo Scientific strives to make available flexible barcoding options. The NEXTflex Rapid DNA-Seq Kit is designed to be used with the NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes and NEXTflex-HT Barcodes when 10 ng or more of starting material is used. These barcodes are available in sets of 6, 12, 24, 48, 96 and 384 unique indexed adapters.
When less than 10 ng of starting material is available, the NEXTflex ChIP-Seq Barcodes and NEXTflex-96 ChIP-Seq Barcodes should be used. These barcodes are available in sets of 6, 12, 24, 48, and 96 unique indexed adapters.
The NEXTflex Rapid DNA-Seq Kit contains enough material to prepare 8 or 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The NEXTflex rapid DNA-seq 2.0 kit is designed to prepare multiplexed libraries for single or paired-end sequencing using Illumina platforms. The enhanced NEXTflex kit simplifies workflow by using master mixed reagents and magnetic bead based cleanup, reducing pipetting and eliminating time-consuming steps in library preparation. The NEXTflex rapid DNA-seq kit produces library preps with a larger number of unique sequencing reads, allowing for robust genome coverage in challenging GC-rich sequences that can introduce bias.
The NEXTflex rapid DNA-seq 2.0 kit bundle offers all the advantages and reagents of the core kit, but with the additional benefit of including unique NEXTflex-HT barcodes for the number of reactions (8, 48, or 96 reaction size kits) in a single-use format. This allows the lab to tie all the necessary components of the library prep workflow to a single lot for ease of purchasing and tracking of consumables.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Complete library prep solution, including size selection beads Bundle kits available with library prep reagents and barcodes Up to 1536 barcodes available upon request Convenient and cost-effective package in a 8, 48, or 96 reaction format Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Kits containing up to 96 unique barcodes Compatible with Illumina HiSeq, MiSeq and GAIIx platforms Prepare single, paired-end and multiplexed genomic DNA libraries Bulk pricing available to meet research goals
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - Illumina Compatible
Applications:
Rapid DNA
Search Terms:
Rapid DNA
Additional Info:
Flexible Multiplexing Options
The NEXTflex Adapters are long, annealed adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. These barcodes can be used with single, paired-end and multiplex reads. Bioo Scientific strives to make available flexible barcoding options. The NEXTflex Rapid DNA-Seq Kit is designed to be used with the NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes and NEXTflex-HT Barcodes when 10 ng or more of starting material is used. These barcodes are available in sets of 6, 12, 24, 48, 96 and 384 unique indexed adapters.
When less than 10 ng of starting material is available, the NEXTflex ChIP-Seq Barcodes and NEXTflex-96 ChIP-Seq Barcodes should be used. These barcodes are available in sets of 6, 12, 24, 48, and 96 unique indexed adapters.
The NEXTflex Rapid DNA-Seq Kit contains enough material to prepare 8 or 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The new NEXTFLEX® Rapid XP V2 DNA-seq kit includes proprietary NEXTFLEX® normalization beads, which provide a consistent mass and sequencing cluster density for all samples in a library pool. This shortens the time needed for quantification and pooling preparation for sequencing in a high throughput lab by up to 3 hours per 96 samples.
[BENEFITS] Working range from 100 pg 1 µg Enzymatic fragmentation with end-repair and A-tailing in one reaction Highly efficient ligation with low adapter dimer formation No need to dilute adapters within range of 1 ng 1 µg Efficient: Automated on the Sciclone® G3 NGSx, Sciclone® G3 NGSx iQ workstations and Zephyr® NGS workstations Up to 1,536 UDI Barcodes available for multiplexing
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Flexible Multiplexing Options We offer a broad range of NEXTflex adapters adapters that improve multiplexing capabilities and offer a solution to flexible experimental setups for both low-level and high-level multiplexing needs. The NEXTflex Rapid XP DNA-seq kit is compatible with NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes, NEXTflex ChIP-Seq Barcodes, and NEXTflex-96 ChIP-Seq Barcodes, and and NEXTflex-HT barcodes. For those high throughput users interested in dual index barcodes, the reagents are also compatible with the NEXTflex Unique Dual Index Barcodes and the NEXTflex Dual-Indexed barcodes.
The NEXTflex Rapid XP DNA-Seq Kit contains enough material to prepare 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The new NEXTFLEX® Rapid XP V2 DNA-seq kit includes proprietary NEXTFLEX® normalization beads, which provide a consistent mass and sequencing cluster density for all samples in a library pool. This shortens the time needed for quantification and pooling preparation for sequencing in a high throughput lab by up to 3 hours per 96 samples.
[BENEFITS] Working range from 100 pg 1 µg Enzymatic fragmentation with end-repair and A-tailing in one reaction Highly efficient ligation with low adapter dimer formation No need to dilute adapters within range of 1 ng 1 µg Efficient: Automated on the Sciclone® G3 NGSx, Sciclone® G3 NGSx iQ workstations and Zephyr® NGS workstations Up to 1,536 UDI Barcodes available for multiplexing
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Flexible Multiplexing Options We offer a broad range of NEXTflex adapters adapters that improve multiplexing capabilities and offer a solution to flexible experimental setups for both low-level and high-level multiplexing needs. The NEXTflex Rapid XP DNA-seq kit is compatible with NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes, NEXTflex ChIP-Seq Barcodes, and NEXTflex-96 ChIP-Seq Barcodes, and and NEXTflex-HT barcodes. For those high throughput users interested in dual index barcodes, the reagents are also compatible with the NEXTflex Unique Dual Index Barcodes and the NEXTflex Dual-Indexed barcodes.
The NEXTflex Rapid XP DNA-Seq Kit contains enough material to prepare 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The new NEXTFLEX® Rapid XP V2 DNA-seq kit includes proprietary NEXTFLEX® normalization beads, which provide a consistent mass and sequencing cluster density for all samples in a library pool. This shortens the time needed for quantification and pooling preparation for sequencing in a high throughput lab by up to 3 hours per 96 samples.
[BENEFITS] Working range from 100 pg 1 µg Enzymatic fragmentation with end-repair and A-tailing in one reaction Highly efficient ligation with low adapter dimer formation No need to dilute adapters within range of 1 ng 1 µg Efficient: Automated on the Sciclone® G3 NGSx, Sciclone® G3 NGSx iQ workstations and Zephyr® NGS workstations Up to 1,536 UDI Barcodes available for multiplexing
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Flexible Multiplexing Options We offer a broad range of NEXTflex adapters adapters that improve multiplexing capabilities and offer a solution to flexible experimental setups for both low-level and high-level multiplexing needs. The NEXTflex Rapid XP DNA-seq kit is compatible with NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes, NEXTflex ChIP-Seq Barcodes, and NEXTflex-96 ChIP-Seq Barcodes, and and NEXTflex-HT barcodes. For those high throughput users interested in dual index barcodes, the reagents are also compatible with the NEXTflex Unique Dual Index Barcodes and the NEXTflex Dual-Indexed barcodes.
The NEXTflex Rapid XP DNA-Seq Kit contains enough material to prepare 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The NEXTflex Rapid XP DNA-Seq kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a highly efficient first step in library generation, which is followed by ligation and PCR (optional for PCR-free workflows). This effective one-tube workflow produces DNA-seq libraries with consistent library size, high yield, low GC-bias, and high coverage.
The kit includes all the required reagents for fragmentation, library prep, and magnetic bead-based cleanup, and it is compatible with your NEXTflex barcodes of choice. This DNA-seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of sample types and sample input amounts from 1 ng to 1 µg to quickly and efficiently generate high-quality libraries for Illumina sequencing.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Reliable: Consistent yields and high-quality sequencing metrics Convenient: Kit includes fragmentation enzyme and cleanup/size selection beads Streamlined: Single step for fragmentation, end-repair, and adenylation with minimal hands-on time Flexible: Chemistry optimized with NEXTflex color-balanced barcodes that allow a wide range of multiplexing (2 up to 384 samples in one run Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Efficient: Automated on the PerkinElmer Sciclone G3 and Zephyr G3 NGS workstation
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - Illumina Compatible
Applications:
Rapid DNA
Search Terms:
Rapid DNA, Rapid XP DNA-Seq
Additional Info:
Flexible Multiplexing Options We offer a broad range of NEXTflex adapters adapters that improve multiplexing capabilities and offer a solution to flexible experimental setups for both low-level and high-level multiplexing needs. The NEXTflex Rapid XP DNA-seq kit is compatible with NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes, NEXTflex ChIP-Seq Barcodes, and NEXTflex-96 ChIP-Seq Barcodes, and and NEXTflex-HT barcodes. For those high throughput users interested in dual index barcodes, the reagents are also compatible with the NEXTflex Unique Dual Index Barcodes and the NEXTflex Dual-Indexed barcodes.
The NEXTflex Rapid XP DNA-Seq Kit contains enough material to prepare 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The NEXTflex Rapid XP DNA-Seq kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a highly efficient first step in library generation, which is followed by ligation and PCR (optional for PCR-free workflows). This effective one-tube workflow produces DNA-seq libraries with consistent library size, high yield, low GC-bias, and high coverage.
The kit includes all the required reagents for fragmentation, library prep, and magnetic bead-based cleanup, and it is compatible with your NEXTflex barcodes of choice. This DNA-seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of sample types and sample input amounts from 1 ng to 1 µg to quickly and efficiently generate high-quality libraries for Illumina sequencing.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Reliable: Consistent yields and high-quality sequencing metrics Convenient: Kit includes fragmentation enzyme and cleanup/size selection beads Streamlined: Single step for fragmentation, end-repair, and adenylation with minimal hands-on time Flexible: Chemistry optimized with NEXTflex color-balanced barcodes that allow a wide range of multiplexing (2 up to 384 samples in one run Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Efficient: Automated on the PerkinElmer Sciclone G3 and Zephyr G3 NGS workstation
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - Illumina Compatible
Applications:
Rapid DNA
Search Terms:
Rapid DNA, Rapid XP DNA-Seq
Additional Info:
Flexible Multiplexing Options We offer a broad range of NEXTflex adapters adapters that improve multiplexing capabilities and offer a solution to flexible experimental setups for both low-level and high-level multiplexing needs. The NEXTflex Rapid XP DNA-seq kit is compatible with NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes, NEXTflex ChIP-Seq Barcodes, and NEXTflex-96 ChIP-Seq Barcodes, and and NEXTflex-HT barcodes. For those high throughput users interested in dual index barcodes, the reagents are also compatible with the NEXTflex Unique Dual Index Barcodes and the NEXTflex Dual-Indexed barcodes.
The NEXTflex Rapid XP DNA-Seq Kit contains enough material to prepare 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The NEXTflex Rapid XP DNA-Seq kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a highly efficient first step in library generation, which is followed by ligation and PCR (optional for PCR-free workflows). This effective one-tube workflow produces DNA-seq libraries with consistent library size, high yield, low GC-bias, and high coverage.
The kit includes all the required reagents for fragmentation, library prep, and magnetic bead-based cleanup, and it is compatible with your NEXTflex barcodes of choice. This DNA-seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of sample types and sample input amounts from 1 ng to 1 µg to quickly and efficiently generate high-quality libraries for Illumina sequencing.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Reliable: Consistent yields and high-quality sequencing metrics Convenient: Kit includes fragmentation enzyme and cleanup/size selection beads Streamlined: Single step for fragmentation, end-repair, and adenylation with minimal hands-on time Flexible: Chemistry optimized with NEXTflex color-balanced barcodes that allow a wide range of multiplexing (2 up to 384 samples in one run Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Efficient: Automated on the PerkinElmer Sciclone G3 and Zephyr G3 NGS workstation
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - Illumina Compatible
Applications:
Rapid DNA
Search Terms:
Rapid DNA, Rapid XP DNA-Seq
Additional Info:
Flexible Multiplexing Options We offer a broad range of NEXTflex adapters adapters that improve multiplexing capabilities and offer a solution to flexible experimental setups for both low-level and high-level multiplexing needs. The NEXTflex Rapid XP DNA-seq kit is compatible with NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes, NEXTflex ChIP-Seq Barcodes, and NEXTflex-96 ChIP-Seq Barcodes, and and NEXTflex-HT barcodes. For those high throughput users interested in dual index barcodes, the reagents are also compatible with the NEXTflex Unique Dual Index Barcodes and the NEXTflex Dual-Indexed barcodes.
The NEXTflex Rapid XP DNA-Seq Kit contains enough material to prepare 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The NEXTflex Rapid XP DNA-Seq kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a highly efficient first step in library generation, which is followed by ligation and PCR (optional for PCR-free workflows). This effective one-tube workflow produces DNA-seq libraries with consistent library size, high yield, low GC-bias, and high coverage.
The kit includes all the required reagents for fragmentation, library prep, and magnetic bead-based cleanup, and it is compatible with your NEXTflex barcodes of choice. This DNA-seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of sample types and sample input amounts from 1 ng to 1 µg to quickly and efficiently generate high-quality libraries for Illumina sequencing.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Reliable: Consistent yields and high-quality sequencing metrics Convenient: Kit includes fragmentation enzyme and cleanup/size selection beads Streamlined: Single step for fragmentation, end-repair, and adenylation with minimal hands-on time Flexible: Chemistry optimized with NEXTflex color-balanced barcodes that allow a wide range of multiplexing (2 up to 384 samples in one run Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Efficient: Automated on the PerkinElmer Sciclone G3 and Zephyr G3 NGS workstation
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - Illumina Compatible
Applications:
Rapid DNA
Search Terms:
Rapid DNA, Rapid XP DNA-Seq
Additional Info:
Flexible Multiplexing Options We offer a broad range of NEXTflex adapters adapters that improve multiplexing capabilities and offer a solution to flexible experimental setups for both low-level and high-level multiplexing needs. The NEXTflex Rapid XP DNA-seq kit is compatible with NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes, NEXTflex ChIP-Seq Barcodes, and NEXTflex-96 ChIP-Seq Barcodes, and and NEXTflex-HT barcodes. For those high throughput users interested in dual index barcodes, the reagents are also compatible with the NEXTflex Unique Dual Index Barcodes and the NEXTflex Dual-Indexed barcodes.
The NEXTflex Rapid XP DNA-Seq Kit contains enough material to prepare 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The NEXTflex Dual Index DNA Barcodes are two kits that each contain 96 dual indexed barcoded adaptors which enable deep multiplexing of NGS sequencing samples compatible with high-throughput automation. They significantly increase scale while reducing costs by allowing the user to pool up to 192 samples in a single flow cell.
Libraries made using the NEXTflex Dual Index DNA Barcodes can be combined with single index libraries using a dual index sequencing read primer.
The NEXTflex Dual Index DNA Barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex Rapid DNA-Seq Kit, the NEXTflex DNA Sequencing Kit and the NEXTflex Methyl DNA-seq Kit and other genomic DNA library prep kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - Dual Index
Applications:
Rapid DNA, Cell Free DNA-Seq
Search Terms:
Rapid DNA, Cell Free DNA-Seq, Dual Index Barcodes
Additional Info:
The NEXTflex Dual Index DNA Barcodes Kits contain 8 reactions each of 96 unique barcodes, enabling the user to multiplex up to 192 samples. This kit is shipped on dry ice.
>>> Compatible with NEXTflex Rapid DNA-Seq, NEXTflex DNA Sequencing Kit, NEXTflex Methyl DNA-Seq Kit and other DNA-Seq applications >>> 8 nt index contained within adapter sequence >>> Increase your sequencing scale by pooling up to 192 samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAIIx, HiSeq, and MiSeq) [These kits ship on dry ice.]
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
NEXTFLEX® Unique Dual Index Barcodes 384 UDI adapters for mitigating sample misassignment due to index hopping
Unique dual index adapters for multiplexing up to 384 Illumina® sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Each lot of the NEXTFLEX® Unique Dual Index barcodes is functionally validated and tested for index purity by sequencing Compatible with both paired-end and single-read Illumina® sequencing
Data Security in Sequencing Applications
The NEXTFLEX® Unique Dual Index Barcodes are barcoded adapters, for sequencing on Illumina® platforms, that provide unprecedented data security in sequencing applications. Multiplexing with NEXTFLEX® Unique Dual Index barcodes drastically increases scale while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane. These barcoded adapters can be used with single read or paired-end read sequencing, and are compatible with NEXTFLEX® library prep kits designed for Illumina® sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
UDIs Prevent Data Ambiguity Caused by Index Hopping
The NEXTFLEX® Unique Dual Index Barcodes utilize two unique 8 nt indexes on each UDI adapter. The 8 nt indices are designed with a Hamming Distance of at least three throughout the entire series, permitting error correction. Each NEXTFLEX® Unique Dual Index Barcode comprises the entire flow cell binding site, sequencing primer binding site, and index sequences, making PCR enrichment optional. Patterned flow cells, such as those found on the NovaSeq® and HiSeq® 3000/4000 platforms have been recognized to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity.
Reduce Run-to-Run Sample Carryover & Sample Crosstalk with UDIs
Even users who dont routinely perform high-throughput multiplexing benefit from the superior design of the NEXTFLEX® Unique Dual Index Barcodes. The ability to avoid using the same set of indices consecutively reduces concerns about run-to-run sample carryover and sample crosstalk. They are designed to specifically address the index hopping, or spread of signal, phenomenon associated with Illumina® platforms that utilize a patterned flow cell.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix (12.5 µM)
* The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc) These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
For automation single-use plates, NEXTFLEX® Unique Dual Index Barcodes may be at a different specified concentration. Please inquire for additional details.
Background Info:
The NEXTflex unique dual index barcodes are barcoded adapters for sequencing on Illumina platforms that provide unprecedented data security in sequencing applications. Increased mis-assignment of indexes has been shown to occur on Illumina sequencing instruments that feature a patterned flow cell and exclusion amplification technology. The NEXTflex unique dual index barcodes are designed to specifically mitigate the index hopping or spread of signal phenomenon associated with Illumina platforms that utilise a patterned flow cell. Index mis-assignment can lead to increased false positive rates, which are especially detrimental to sensitive applications. Multiplexing with NEXTflex unique dual index barcodes drastically increases processing capacity while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane.
Illumina sequencing instruments that utilise a patterned flow cell and exclusion amplification technology is known to suffer from increased levels of sample mis-assignment. The use of NEXTflex unique dual index barcodes prevents such mis-assigned reads from appearing in final data sets allowing for the highest assurance of data integrity.
Product Type:
NGS - DNA - Dual Index
Applications:
Rapid DNA, mtDNA-Seq, ChIP-Seq
Search Terms:
Rapid DNA, Cell Free DNA-Seq, Dual Index Barcodes, NEXTflex Unique Dual Index Barcoded Adapters 384 UDI Set
Additional Info:
Key Features:
Unique dual index adapters for multiplexing up to 96 Illumina sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Decreases level of mis-assigned reads in sequencing data Compatible with both paired-end and single-read Illumina sequencing
[These kits ship on dry ice.]
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
NEXTFLEX® Unique Dual Index Barcodes 384 UDI adapters for mitigating sample misassignment due to index hopping
Unique dual index adapters for multiplexing up to 384 Illumina® sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Each lot of the NEXTFLEX® Unique Dual Index barcodes is functionally validated and tested for index purity by sequencing Compatible with both paired-end and single-read Illumina® sequencing
Data Security in Sequencing Applications
The NEXTFLEX® Unique Dual Index Barcodes are barcoded adapters, for sequencing on Illumina® platforms, that provide unprecedented data security in sequencing applications. Multiplexing with NEXTFLEX® Unique Dual Index barcodes drastically increases scale while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane. These barcoded adapters can be used with single read or paired-end read sequencing, and are compatible with NEXTFLEX® library prep kits designed for Illumina® sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
UDIs Prevent Data Ambiguity Caused by Index Hopping
The NEXTFLEX® Unique Dual Index Barcodes utilize two unique 8 nt indexes on each UDI adapter. The 8 nt indices are designed with a Hamming Distance of at least three throughout the entire series, permitting error correction. Each NEXTFLEX® Unique Dual Index Barcode comprises the entire flow cell binding site, sequencing primer binding site, and index sequences, making PCR enrichment optional. Patterned flow cells, such as those found on the NovaSeq® and HiSeq® 3000/4000 platforms have been recognized to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity.
Reduce Run-to-Run Sample Carryover & Sample Crosstalk with UDIs
Even users who dont routinely perform high-throughput multiplexing benefit from the superior design of the NEXTFLEX® Unique Dual Index Barcodes. The ability to avoid using the same set of indices consecutively reduces concerns about run-to-run sample carryover and sample crosstalk. They are designed to specifically address the index hopping, or spread of signal, phenomenon associated with Illumina® platforms that utilize a patterned flow cell.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix (12.5 µM)
* The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc) These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
For automation single-use plates, NEXTFLEX® Unique Dual Index Barcodes may be at a different specified concentration. Please inquire for additional details.
Background Info:
The NEXTflex unique dual index barcodes are barcoded adapters for sequencing on Illumina platforms that provide unprecedented data security in sequencing applications. Increased mis-assignment of indexes has been shown to occur on Illumina sequencing instruments that feature a patterned flow cell and exclusion amplification technology. The NEXTflex unique dual index barcodes are designed to specifically mitigate the index hopping or spread of signal phenomenon associated with Illumina platforms that utilise a patterned flow cell. Index mis-assignment can lead to increased false positive rates, which are especially detrimental to sensitive applications. Multiplexing with NEXTflex unique dual index barcodes drastically increases processing capacity while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane.
Illumina sequencing instruments that utilise a patterned flow cell and exclusion amplification technology is known to suffer from increased levels of sample mis-assignment. The use of NEXTflex unique dual index barcodes prevents such mis-assigned reads from appearing in final data sets allowing for the highest assurance of data integrity.
Product Type:
NGS - DNA - Dual Index
Applications:
Rapid DNA, mtDNA-Seq, ChIP-Seq
Search Terms:
Rapid DNA, Cell Free DNA-Seq, Dual Index Barcodes, NEXTflex Unique Dual Index Barcoded Adapters 384 UDI Set
Additional Info:
Key Features:
Unique dual index adapters for multiplexing up to 96 Illumina sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Decreases level of mis-assigned reads in sequencing data Compatible with both paired-end and single-read Illumina sequencing
[These kits ship on dry ice.]
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
NEXTFLEX® Unique Dual Index Barcodes 384 UDI adapters for mitigating sample misassignment due to index hopping
Unique dual index adapters for multiplexing up to 384 Illumina® sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Each lot of the NEXTFLEX® Unique Dual Index barcodes is functionally validated and tested for index purity by sequencing Compatible with both paired-end and single-read Illumina® sequencing
Data Security in Sequencing Applications
The NEXTFLEX® Unique Dual Index Barcodes are barcoded adapters, for sequencing on Illumina® platforms, that provide unprecedented data security in sequencing applications. Multiplexing with NEXTFLEX® Unique Dual Index barcodes drastically increases scale while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane. These barcoded adapters can be used with single read or paired-end read sequencing, and are compatible with NEXTFLEX® library prep kits designed for Illumina® sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
UDIs Prevent Data Ambiguity Caused by Index Hopping
The NEXTFLEX® Unique Dual Index Barcodes utilize two unique 8 nt indexes on each UDI adapter. The 8 nt indices are designed with a Hamming Distance of at least three throughout the entire series, permitting error correction. Each NEXTFLEX® Unique Dual Index Barcode comprises the entire flow cell binding site, sequencing primer binding site, and index sequences, making PCR enrichment optional. Patterned flow cells, such as those found on the NovaSeq® and HiSeq® 3000/4000 platforms have been recognized to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity.
Reduce Run-to-Run Sample Carryover & Sample Crosstalk with UDIs
Even users who dont routinely perform high-throughput multiplexing benefit from the superior design of the NEXTFLEX® Unique Dual Index Barcodes. The ability to avoid using the same set of indices consecutively reduces concerns about run-to-run sample carryover and sample crosstalk. They are designed to specifically address the index hopping, or spread of signal, phenomenon associated with Illumina® platforms that utilize a patterned flow cell.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix (12.5 µM)
* The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc) These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
For automation single-use plates, NEXTFLEX® Unique Dual Index Barcodes may be at a different specified concentration. Please inquire for additional details.
Background Info:
The NEXTflex unique dual index barcodes are barcoded adapters for sequencing on Illumina platforms that provide unprecedented data security in sequencing applications. Increased mis-assignment of indexes has been shown to occur on Illumina sequencing instruments that feature a patterned flow cell and exclusion amplification technology. The NEXTflex unique dual index barcodes are designed to specifically mitigate the index hopping or spread of signal phenomenon associated with Illumina platforms that utilise a patterned flow cell. Index mis-assignment can lead to increased false positive rates, which are especially detrimental to sensitive applications. Multiplexing with NEXTflex unique dual index barcodes drastically increases processing capacity while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane.
Illumina sequencing instruments that utilise a patterned flow cell and exclusion amplification technology is known to suffer from increased levels of sample mis-assignment. The use of NEXTflex unique dual index barcodes prevents such mis-assigned reads from appearing in final data sets allowing for the highest assurance of data integrity.
Product Type:
NGS - DNA - Dual Index
Applications:
Rapid DNA, mtDNA-Seq, ChIP-Seq
Search Terms:
Rapid DNA, Cell Free DNA-Seq, Dual Index Barcodes, NEXTflex Unique Dual Index Barcoded Adapters 384 UDI Set
Additional Info:
Key Features:
Unique dual index adapters for multiplexing up to 96 Illumina sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Decreases level of mis-assigned reads in sequencing data Compatible with both paired-end and single-read Illumina sequencing
[These kits ship on dry ice.]
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The NEXTflex Unique Dual Index Barcodes Kit contains 96 unique dual-index barcodes, enabling users to multiplex up to 96 samples per flow cell lane. Each lot of the NEXTflex Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing. NEXTflex Unique Dual Index Barcodes are supplied in 96-well plates with each well containing 2 reactions of each barcode. These kits ship on dry ice.
Background Info:
The NEXTflex unique dual index barcodes are barcoded adapters for sequencing on Illumina platforms that provide unprecedented data security in sequencing applications. Increased mis-assignment of indexes has been shown to occur on Illumina sequencing instruments that feature a patterned flow cell and exclusion amplification technology. The NEXTflex unique dual index barcodes are designed to specifically mitigate the index hopping or spread of signal phenomenon associated with Illumina platforms that utilise a patterned flow cell. Index mis-assignment can lead to increased false positive rates, which are especially detrimental to sensitive applications. Multiplexing with NEXTflex unique dual index barcodes drastically increases processing capacity while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane.
Illumina sequencing instruments that utilise a patterned flow cell and exclusion amplification technology is known to suffer from increased levels of sample mis-assignment. The use of NEXTflex unique dual index barcodes prevents such mis-assigned reads from appearing in final data sets allowing for the highest assurance of data integrity.
Product Type:
NGS - DNA - Dual Index
Applications:
Rapid DNA, mtDNA-Seq, ChIP-Seq
Search Terms:
Rapid DNA, Cell Free DNA-Seq, Dual Index Barcodes
Additional Info:
Key Features:
Unique dual index adapters for multiplexing up to 96 Illumina sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Decreases level of mis-assigned reads in sequencing data Compatible with both paired-end and single-read Illumina sequencing
[These kits ship on dry ice.]
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The NEXTflex Unique Dual Index Barcodes Kit contains 96 unique dual-index barcodes, enabling users to multiplex up to 96 samples per flow cell lane. Each lot of the NEXTflex Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing. NEXTflex Unique Dual Index Barcodes are supplied in 96-well plates with each well containing 2 reactions of each barcode. These kits ship on dry ice.
Background Info:
The NEXTflex unique dual index barcodes are barcoded adapters for sequencing on Illumina platforms that provide unprecedented data security in sequencing applications. Increased mis-assignment of indexes has been shown to occur on Illumina sequencing instruments that feature a patterned flow cell and exclusion amplification technology. The NEXTflex unique dual index barcodes are designed to specifically mitigate the index hopping or spread of signal phenomenon associated with Illumina platforms that utilise a patterned flow cell. Index mis-assignment can lead to increased false positive rates, which are especially detrimental to sensitive applications. Multiplexing with NEXTflex unique dual index barcodes drastically increases processing capacity while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane.
Illumina sequencing instruments that utilise a patterned flow cell and exclusion amplification technology is known to suffer from increased levels of sample mis-assignment. The use of NEXTflex unique dual index barcodes prevents such mis-assigned reads from appearing in final data sets allowing for the highest assurance of data integrity.
Product Type:
NGS - DNA - Dual Index
Applications:
Rapid DNA, mtDNA-Seq, ChIP-Seq
Search Terms:
Rapid DNA, Cell Free DNA-Seq, Dual Index Barcodes
Additional Info:
Key Features:
Unique dual index adapters for multiplexing up to 96 Illumina sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Decreases level of mis-assigned reads in sequencing data Compatible with both paired-end and single-read Illumina sequencing
[These kits ship on dry ice.]
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
NEXTFLEX® Unique Dual Index Barcodes 384 UDI adapters for mitigating sample misassignment due to index hopping
Unique dual index adapters for multiplexing up to 384 Illumina® sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Each lot of the NEXTFLEX® Unique Dual Index barcodes is functionally validated and tested for index purity by sequencing Compatible with both paired-end and single-read Illumina® sequencing
Data Security in Sequencing Applications
The NEXTFLEX® Unique Dual Index Barcodes are barcoded adapters, for sequencing on Illumina® platforms, that provide unprecedented data security in sequencing applications. Multiplexing with NEXTFLEX® Unique Dual Index barcodes drastically increases scale while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane. These barcoded adapters can be used with single read or paired-end read sequencing, and are compatible with NEXTFLEX® library prep kits designed for Illumina® sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
UDIs Prevent Data Ambiguity Caused by Index Hopping
The NEXTFLEX® Unique Dual Index Barcodes utilize two unique 8 nt indexes on each UDI adapter. The 8 nt indices are designed with a Hamming Distance of at least three throughout the entire series, permitting error correction. Each NEXTFLEX® Unique Dual Index Barcode comprises the entire flow cell binding site, sequencing primer binding site, and index sequences, making PCR enrichment optional. Patterned flow cells, such as those found on the NovaSeq® and HiSeq® 3000/4000 platforms have been recognized to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity.
Reduce Run-to-Run Sample Carryover & Sample Crosstalk with UDIs
Even users who dont routinely perform high-throughput multiplexing benefit from the superior design of the NEXTFLEX® Unique Dual Index Barcodes. The ability to avoid using the same set of indices consecutively reduces concerns about run-to-run sample carryover and sample crosstalk. They are designed to specifically address the index hopping, or spread of signal, phenomenon associated with Illumina® platforms that utilize a patterned flow cell.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix (12.5 µM)
* The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc) These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
For automation single-use plates, NEXTFLEX® Unique Dual Index Barcodes may be at a different specified concentration. Please inquire for additional details.
Background Info:
The NEXTflex unique dual index barcodes are barcoded adapters for sequencing on Illumina platforms that provide unprecedented data security in sequencing applications. Increased mis-assignment of indexes has been shown to occur on Illumina sequencing instruments that feature a patterned flow cell and exclusion amplification technology. The NEXTflex unique dual index barcodes are designed to specifically mitigate the index hopping or spread of signal phenomenon associated with Illumina platforms that utilise a patterned flow cell. Index mis-assignment can lead to increased false positive rates, which are especially detrimental to sensitive applications. Multiplexing with NEXTflex unique dual index barcodes drastically increases processing capacity while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane.
Illumina sequencing instruments that utilise a patterned flow cell and exclusion amplification technology is known to suffer from increased levels of sample mis-assignment. The use of NEXTflex unique dual index barcodes prevents such mis-assigned reads from appearing in final data sets allowing for the highest assurance of data integrity.
Product Type:
NGS - DNA - Dual Index
Applications:
Rapid DNA, mtDNA-Seq, ChIP-Seq
Search Terms:
Rapid DNA, Cell Free DNA-Seq, Dual Index Barcodes, NEXTflex Unique Dual Index Barcoded Adapters 384 UDI Set
Additional Info:
Key Features:
Unique dual index adapters for multiplexing up to 96 Illumina sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Decreases level of mis-assigned reads in sequencing data Compatible with both paired-end and single-read Illumina sequencing
[These kits ship on dry ice.]
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The NEXTflex-96 DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex-96 DNA Barcodes kits accomplish this by using an indexed adapter with an 8 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. The NEXTflex-96 DNA Barcode kits are supplied in either a 96-well format (cat # 514105) or in microfuge tubes (cat # 514106). Do you want to index you samples but dont want to use 96 different barcodes? We also offer the NEXTflex DNA Barcodes in sets of 6, 12, 24 and 48 barcodes. These barcodes can be used with DNA-Seq kits, Rapid DNA-Seq kits and the Methyl-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Rapid DNA, Methyl-Seq, SeqCap EZ Target Capture, SureSelect, Cell Free DNA-Seq Kit for Illumina, NEXTflex Pre-Capture Combo, barcode blockers
Additional Info:
The NEXTflex-96 DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex-96 DNA Barcodes kits accomplish this by using an indexed adapter with an 8 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. The NEXTflex-96 DNA Barcode kits are supplied in either a 96-well format (cat # 514105) or in microfuge tubes (cat # 514106). Do you want to index you samples but dont want to use 96 different barcodes? We also offer the NEXTflex DNA Barcodes in sets of 6, 12, 24 and 48 barcodes.
These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits, other genomic DNA library prep and mRNA-Seq protocols and the NEXTflex Rapid DNA-Seq Kit.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 8 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Each kit comes with 8 reactions each of 96 multiplexed barcodes (768 reactions per kit) >>> Compatible with all DNA-Seq and mRNA-Seq application >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 1000/2000) >>> The kits are supplied in either a 96-well plate format or 96 microfuge tubes
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The NEXTflex DNA Barcodes Kits contains 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with DNA-Seq kits, Rapid DNA-Seq kits and the Methyl-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Rapid DNA, Methyl-Seq, SeqCap EZ Target Capture, SureSelect, Cell Free DNA-Seq Kit for Illumina, barcode blockers
Additional Info:
NEXTflex DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits and PCR-free sequencing workflows and the NEXTflex Rapid DNA-Seq Kit.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 6 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Up to 48 multiplexed samples (up to 384 reactions per kit) >>> Compatible with PCR-free sequencing workflows >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The NEXTflex DNA Barcodes Kits contains 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with DNA-Seq kits, Rapid DNA-Seq kits and the Methyl-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Rapid DNA, Methyl-Seq, SeqCap EZ Target Capture, SureSelect, Cell Free DNA-Seq Kit for Illumina, barcode blockers
Additional Info:
NEXTflex DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits and PCR-free sequencing workflows and the NEXTflex Rapid DNA-Seq Kit.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 6 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Up to 48 multiplexed samples (up to 384 reactions per kit) >>> Compatible with PCR-free sequencing workflows >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
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