The NEXTflex DNA Barcodes Kits contains 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with DNA-Seq kits, Rapid DNA-Seq kits and the Methyl-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Rapid DNA, Methyl-Seq, SeqCap EZ Target Capture, SureSelect, Cell Free DNA-Seq Kit for Illumina, barcode blockers
Additional Info:
NEXTflex DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits and PCR-free sequencing workflows and the NEXTflex Rapid DNA-Seq Kit.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 6 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Up to 48 multiplexed samples (up to 384 reactions per kit) >>> Compatible with PCR-free sequencing workflows >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514102
The NEXTflex DNA Barcodes Kits contains 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with DNA-Seq kits, Rapid DNA-Seq kits and the Methyl-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Rapid DNA, Methyl-Seq, SeqCap EZ Target Capture, SureSelect, Cell Free DNA-Seq Kit for Illumina, barcode blockers
Additional Info:
NEXTflex DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits and PCR-free sequencing workflows and the NEXTflex Rapid DNA-Seq Kit.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 6 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Up to 48 multiplexed samples (up to 384 reactions per kit) >>> Compatible with PCR-free sequencing workflows >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514103
The NEXTflex DNA Barcodes Kits contains 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with DNA-Seq kits, Rapid DNA-Seq kits and the Methyl-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Rapid DNA, Methyl-Seq, SeqCap EZ Target Capture, SureSelect, Cell Free DNA-Seq Kit for Illumina, barcode blockers
Additional Info:
NEXTflex DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits and PCR-free sequencing workflows and the NEXTflex Rapid DNA-Seq Kit.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 6 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Up to 48 multiplexed samples (up to 384 reactions per kit) >>> Compatible with PCR-free sequencing workflows >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514104
The NEXTflex DNA Barcodes Kits contains 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with DNA-Seq kits, Rapid DNA-Seq kits and the Methyl-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Rapid DNA, Methyl-Seq, SeqCap EZ Target Capture, SureSelect, Cell Free DNA-Seq Kit for Illumina, barcode blockers
Additional Info:
NEXTflex DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits and PCR-free sequencing workflows and the NEXTflex Rapid DNA-Seq Kit.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 6 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Up to 48 multiplexed samples (up to 384 reactions per kit) >>> Compatible with PCR-free sequencing workflows >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514101
The NEXTflex Dual Index DNA Barcodes are two kits that each contain 96 dual indexed barcoded adaptors which enable deep multiplexing of NGS sequencing samples compatible with high-throughput automation. They significantly increase scale while reducing costs by allowing the user to pool up to 192 samples in a single flow cell.
Libraries made using the NEXTflex Dual Index DNA Barcodes can be combined with single index libraries using a dual index sequencing read primer.
The NEXTflex Dual Index DNA Barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex Rapid DNA-Seq Kit, the NEXTflex DNA Sequencing Kit and the NEXTflex Methyl DNA-seq Kit and other genomic DNA library prep kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - Dual Index
Applications:
Rapid DNA, Cell Free DNA-Seq
Search Terms:
Rapid DNA, Cell Free DNA-Seq, Dual Index Barcodes
Additional Info:
The NEXTflex Dual Index DNA Barcodes Kits contain 8 reactions each of 96 unique barcodes, enabling the user to multiplex up to 192 samples. This kit is shipped on dry ice.
>>> Compatible with NEXTflex Rapid DNA-Seq, NEXTflex DNA Sequencing Kit, NEXTflex Methyl DNA-Seq Kit and other DNA-Seq applications >>> 8 nt index contained within adapter sequence >>> Increase your sequencing scale by pooling up to 192 samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAIIx, HiSeq, and MiSeq) [These kits ship on dry ice.]
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514160
The NEXTflex rapid DNA-seq 2.0 kit is designed to prepare multiplexed libraries for single or paired-end sequencing using Illumina platforms. The enhanced NEXTflex kit simplifies workflow by using master mixed reagents and magnetic bead based cleanup, reducing pipetting and eliminating time-consuming steps in library preparation. The NEXTflex rapid DNA-seq kit produces library preps with a larger number of unique sequencing reads, allowing for robust genome coverage in challenging GC-rich sequences that can introduce bias.
The NEXTflex rapid DNA-seq 2.0 kit bundle offers all the advantages and reagents of the core kit, but with the additional benefit of including unique NEXTflex-HT barcodes for the number of reactions (8, 48, or 96 reaction size kits) in a single-use format. This allows the lab to tie all the necessary components of the library prep workflow to a single lot for ease of purchasing and tracking of consumables.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Complete library prep solution, including size selection beads Bundle kits available with library prep reagents and barcodes Up to 384 barcodes available upon request Convenient and cost-effective package in a 8, 48, or 96 reaction format Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Kits containing up to 96 unique barcodes Compatible with Illumina HiSeq, MiSeq and GAIIx platforms Prepare single, paired-end and multiplexed genomic DNA libraries Bulk pricing available to meet research goals
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex Adapters are long, annealed adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. These barcodes can be used with single, paired-end and multiplex reads. Bioo Scientific strives to make available flexible barcoding options. The NEXTflex Rapid DNA-Seq Kit is designed to be used with the NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes and NEXTflex-HT Barcodes when 10 ng or more of starting material is used. These barcodes are available in sets of 6, 12, 24, 48, 96 and 384 unique indexed adapters.
When less than 10 ng of starting material is available, the NEXTflex ChIP-Seq Barcodes and NEXTflex-96 ChIP-Seq Barcodes should be used. These barcodes are available in sets of 6, 12, 24, 48, and 96 unique indexed adapters.
The NEXTflex Rapid DNA-Seq Kit contains enough material to prepare 8 or 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Bioo Scientifics NEXTflex Rapid DNA-Seq Kit is a rapid DNA library prep kit producing libraries for Illumina sequencing from as little as 1 ng of DNA in two hours. This kit is ideal for use with genomic DNA, FFPE samples, ChIP DNA, upstream target capture samples, pooled amplicons and low input clinical samples. This DNA-Seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of input DNA amounts from 1 ng to 1 µg. The optimized protocol allows for fast generation of non-size-selected libraries and size-selected libraries using reproducible gel-free double SPRI selection.
The NEXTflex Rapid DNA-Seq Kit incorporates Enhanced Adapter Ligation Technology, which facilitates ligation of long adapters, resulting in longer and more diverse sequencing reads. Bioo Scientifics NEXTflex Ligation and Polymerase reaction mixes ensure the highest quality libraries for superior performance. Study results in the white paper, "Optimizing Library Preparation: Enhanced Adapter Ligation Technology", demonstrate the superior ligation efficiency of the NEXTflex Rapid DNA-Seq Library Prep Kit for creating NGS libraries for Illumina sequencing.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Flexible amounts of input DNA from 1 ng to 1 µg Fast workflow requiring 2 hours or less, with minimal hands-on time Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads Creates libraries with less bias than alternative methods Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Kits containing up to 96 unique barcodes Compatible with Illumina HiSeq, MiSeq and GAIIx platforms Prepare single, paired-end and multiplexed genomic DNA libraries Bulk pricing available to meet research goals
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex Adapters are long, annealed adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. These barcodes can be used with single, paired-end and multiplex reads. Bioo Scientific strives to make available flexible barcoding options. The NEXTflex Rapid DNA-Seq Kit is designed to be used with the NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes and NEXTflex-HT Barcodes when 10 ng or more of starting material is used. These barcodes are available in sets of 6, 12, 24, 48, 96 and 384 unique indexed adapters.
When less than 10 ng of starting material is available, the NEXTflex ChIP-Seq Barcodes and NEXTflex-96 ChIP-Seq Barcodes should be used. These barcodes are available in sets of 6, 12, 24, 48, and 96 unique indexed adapters.
The NEXTflex Rapid DNA-Seq Kit contains enough material to prepare 8 or 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Bioo Scientifics NEXTflex Rapid DNA-Seq Kit is a rapid DNA library prep kit producing libraries for Illumina sequencing from as little as 1 ng of DNA in two hours. This kit is ideal for use with genomic DNA, FFPE samples, ChIP DNA, upstream target capture samples, pooled amplicons and low input clinical samples. This DNA-Seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of input DNA amounts from 1 ng to 1 µg. The optimized protocol allows for fast generation of non-size-selected libraries and size-selected libraries using reproducible gel-free double SPRI selection.
The NEXTflex Rapid DNA-Seq Kit incorporates Enhanced Adapter Ligation Technology, which facilitates ligation of long adapters, resulting in longer and more diverse sequencing reads. Bioo Scientifics NEXTflex Ligation and Polymerase reaction mixes ensure the highest quality libraries for superior performance. Study results in the white paper, "Optimizing Library Preparation: Enhanced Adapter Ligation Technology", demonstrate the superior ligation efficiency of the NEXTflex Rapid DNA-Seq Library Prep Kit for creating NGS libraries for Illumina sequencing.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Flexible amounts of input DNA from 1 ng to 1 µg Fast workflow requiring 2 hours or less, with minimal hands-on time Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads Creates libraries with less bias than alternative methods Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Kits containing up to 96 unique barcodes Compatible with Illumina HiSeq, MiSeq and GAIIx platforms Prepare single, paired-end and multiplexed genomic DNA libraries Bulk pricing available to meet research goals
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex Adapters are long, annealed adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. These barcodes can be used with single, paired-end and multiplex reads. Bioo Scientific strives to make available flexible barcoding options. The NEXTflex Rapid DNA-Seq Kit is designed to be used with the NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes and NEXTflex-HT Barcodes when 10 ng or more of starting material is used. These barcodes are available in sets of 6, 12, 24, 48, 96 and 384 unique indexed adapters.
When less than 10 ng of starting material is available, the NEXTflex ChIP-Seq Barcodes and NEXTflex-96 ChIP-Seq Barcodes should be used. These barcodes are available in sets of 6, 12, 24, 48, and 96 unique indexed adapters.
The NEXTflex Rapid DNA-Seq Kit contains enough material to prepare 8 or 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
The NEXTflex Rapid XP DNA-Seq kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a highly efficient first step in library generation, which is followed by ligation and PCR (optional for PCR-free workflows). This effective one-tube workflow produces DNA-seq libraries with consistent library size, high yield, low GC-bias, and high coverage.
The kit includes all the required reagents for fragmentation, library prep, and magnetic bead-based cleanup, and it is compatible with your NEXTflex barcodes of choice. This DNA-seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of sample types and sample input amounts from 1 ng to 1 µg to quickly and efficiently generate high-quality libraries for Illumina sequencing.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Reliable: Consistent yields and high-quality sequencing metrics Convenient: Kit includes fragmentation enzyme and cleanup/size selection beads Streamlined: Single step for fragmentation, end-repair, and adenylation with minimal hands-on time Flexible: Chemistry optimized with NEXTflex color-balanced barcodes that allow a wide range of multiplexing (2 up to 384 samples in one run Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Efficient: Automated on the PerkinElmer Sciclone G3 and Zephyr G3 NGS workstation
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Flexible Multiplexing Options We offer a broad range of NEXTflex adapters adapters that improve multiplexing capabilities and offer a solution to flexible experimental setups for both low-level and high-level multiplexing needs. The NEXTflex Rapid XP DNA-seq kit is compatible with NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes, NEXTflex ChIP-Seq Barcodes, and NEXTflex-96 ChIP-Seq Barcodes, and and NEXTflex-HT barcodes. For those high throughput users interested in dual index barcodes, the reagents are also compatible with the NEXTflex Unique Dual Index Barcodes and the NEXTflex Dual-Indexed barcodes.
The NEXTflex Rapid XP DNA-Seq Kit contains enough material to prepare 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
The NEXTflex Rapid XP DNA-Seq kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a highly efficient first step in library generation, which is followed by ligation and PCR (optional for PCR-free workflows). This effective one-tube workflow produces DNA-seq libraries with consistent library size, high yield, low GC-bias, and high coverage.
The kit includes all the required reagents for fragmentation, library prep, and magnetic bead-based cleanup, and it is compatible with your NEXTflex barcodes of choice. This DNA-seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of sample types and sample input amounts from 1 ng to 1 µg to quickly and efficiently generate high-quality libraries for Illumina sequencing.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Reliable: Consistent yields and high-quality sequencing metrics Convenient: Kit includes fragmentation enzyme and cleanup/size selection beads Streamlined: Single step for fragmentation, end-repair, and adenylation with minimal hands-on time Flexible: Chemistry optimized with NEXTflex color-balanced barcodes that allow a wide range of multiplexing (2 up to 384 samples in one run Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Efficient: Automated on the PerkinElmer Sciclone G3 and Zephyr G3 NGS workstation
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Flexible Multiplexing Options We offer a broad range of NEXTflex adapters adapters that improve multiplexing capabilities and offer a solution to flexible experimental setups for both low-level and high-level multiplexing needs. The NEXTflex Rapid XP DNA-seq kit is compatible with NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes, NEXTflex ChIP-Seq Barcodes, and NEXTflex-96 ChIP-Seq Barcodes, and and NEXTflex-HT barcodes. For those high throughput users interested in dual index barcodes, the reagents are also compatible with the NEXTflex Unique Dual Index Barcodes and the NEXTflex Dual-Indexed barcodes.
The NEXTflex Rapid XP DNA-Seq Kit contains enough material to prepare 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
The NEXTflex Rapid XP DNA-Seq kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a highly efficient first step in library generation, which is followed by ligation and PCR (optional for PCR-free workflows). This effective one-tube workflow produces DNA-seq libraries with consistent library size, high yield, low GC-bias, and high coverage.
The kit includes all the required reagents for fragmentation, library prep, and magnetic bead-based cleanup, and it is compatible with your NEXTflex barcodes of choice. This DNA-seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of sample types and sample input amounts from 1 ng to 1 µg to quickly and efficiently generate high-quality libraries for Illumina sequencing.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Reliable: Consistent yields and high-quality sequencing metrics Convenient: Kit includes fragmentation enzyme and cleanup/size selection beads Streamlined: Single step for fragmentation, end-repair, and adenylation with minimal hands-on time Flexible: Chemistry optimized with NEXTflex color-balanced barcodes that allow a wide range of multiplexing (2 up to 384 samples in one run Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Efficient: Automated on the PerkinElmer Sciclone G3 and Zephyr G3 NGS workstation
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Flexible Multiplexing Options We offer a broad range of NEXTflex adapters adapters that improve multiplexing capabilities and offer a solution to flexible experimental setups for both low-level and high-level multiplexing needs. The NEXTflex Rapid XP DNA-seq kit is compatible with NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes, NEXTflex ChIP-Seq Barcodes, and NEXTflex-96 ChIP-Seq Barcodes, and and NEXTflex-HT barcodes. For those high throughput users interested in dual index barcodes, the reagents are also compatible with the NEXTflex Unique Dual Index Barcodes and the NEXTflex Dual-Indexed barcodes.
The NEXTflex Rapid XP DNA-Seq Kit contains enough material to prepare 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
The NEXTflex Rapid XP DNA-Seq kit combines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a highly efficient first step in library generation, which is followed by ligation and PCR (optional for PCR-free workflows). This effective one-tube workflow produces DNA-seq libraries with consistent library size, high yield, low GC-bias, and high coverage.
The kit includes all the required reagents for fragmentation, library prep, and magnetic bead-based cleanup, and it is compatible with your NEXTflex barcodes of choice. This DNA-seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of sample types and sample input amounts from 1 ng to 1 µg to quickly and efficiently generate high-quality libraries for Illumina sequencing.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Reliable: Consistent yields and high-quality sequencing metrics Convenient: Kit includes fragmentation enzyme and cleanup/size selection beads Streamlined: Single step for fragmentation, end-repair, and adenylation with minimal hands-on time Flexible: Chemistry optimized with NEXTflex color-balanced barcodes that allow a wide range of multiplexing (2 up to 384 samples in one run Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Efficient: Automated on the PerkinElmer Sciclone G3 and Zephyr G3 NGS workstation
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Flexible Multiplexing Options We offer a broad range of NEXTflex adapters adapters that improve multiplexing capabilities and offer a solution to flexible experimental setups for both low-level and high-level multiplexing needs. The NEXTflex Rapid XP DNA-seq kit is compatible with NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes, NEXTflex ChIP-Seq Barcodes, and NEXTflex-96 ChIP-Seq Barcodes, and and NEXTflex-HT barcodes. For those high throughput users interested in dual index barcodes, the reagents are also compatible with the NEXTflex Unique Dual Index Barcodes and the NEXTflex Dual-Indexed barcodes.
The NEXTflex Rapid XP DNA-Seq Kit contains enough material to prepare 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
The NEXTflex Unique Dual Index Barcodes Kit contains 96 unique dual-index barcodes, enabling users to multiplex up to 96 samples per flow cell lane. Each lot of the NEXTflex Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing. NEXTflex Unique Dual Index Barcodes are supplied in 96-well plates with each well containing 2 reactions of each barcode. These kits ship on dry ice.
Background Info:
The NEXTflex unique dual index barcodes are barcoded adapters for sequencing on Illumina platforms that provide unprecedented data security in sequencing applications. Increased mis-assignment of indexes has been shown to occur on Illumina sequencing instruments that feature a patterned flow cell and exclusion amplification technology. The NEXTflex unique dual index barcodes are designed to specifically mitigate the index hopping or spread of signal phenomenon associated with Illumina platforms that utilise a patterned flow cell. Index mis-assignment can lead to increased false positive rates, which are especially detrimental to sensitive applications. Multiplexing with NEXTflex unique dual index barcodes drastically increases processing capacity while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane.
Illumina sequencing instruments that utilise a patterned flow cell and exclusion amplification technology is known to suffer from increased levels of sample mis-assignment. The use of NEXTflex unique dual index barcodes prevents such mis-assigned reads from appearing in final data sets allowing for the highest assurance of data integrity.
Unique dual index adapters for multiplexing up to 96 Illumina sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Decreases level of mis-assigned reads in sequencing data Compatible with both paired-end and single-read Illumina sequencing
[These kits ship on dry ice.]
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex Unique Dual Index Barcodes Kit contains 96 unique dual-index barcodes, enabling users to multiplex up to 96 samples per flow cell lane. Each lot of the NEXTflex Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing. NEXTflex Unique Dual Index Barcodes are supplied in 96-well plates with each well containing 2 reactions of each barcode. These kits ship on dry ice.
Background Info:
The NEXTflex unique dual index barcodes are barcoded adapters for sequencing on Illumina platforms that provide unprecedented data security in sequencing applications. Increased mis-assignment of indexes has been shown to occur on Illumina sequencing instruments that feature a patterned flow cell and exclusion amplification technology. The NEXTflex unique dual index barcodes are designed to specifically mitigate the index hopping or spread of signal phenomenon associated with Illumina platforms that utilise a patterned flow cell. Index mis-assignment can lead to increased false positive rates, which are especially detrimental to sensitive applications. Multiplexing with NEXTflex unique dual index barcodes drastically increases processing capacity while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane.
Illumina sequencing instruments that utilise a patterned flow cell and exclusion amplification technology is known to suffer from increased levels of sample mis-assignment. The use of NEXTflex unique dual index barcodes prevents such mis-assigned reads from appearing in final data sets allowing for the highest assurance of data integrity.
Unique dual index adapters for multiplexing up to 96 Illumina sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Decreases level of mis-assigned reads in sequencing data Compatible with both paired-end and single-read Illumina sequencing
[These kits ship on dry ice.]
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex Unique Dual Index Barcodes Kit contains 96 unique dual-index barcodes, enabling users to multiplex up to 96 samples per flow cell lane. Each lot of the NEXTflex Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing. NEXTflex Unique Dual Index Barcodes are supplied in 96-well plates with each well containing 2 reactions of each barcode. These kits ship on dry ice.
Background Info:
The NEXTflex unique dual index barcodes are barcoded adapters for sequencing on Illumina platforms that provide unprecedented data security in sequencing applications. Increased mis-assignment of indexes has been shown to occur on Illumina sequencing instruments that feature a patterned flow cell and exclusion amplification technology. The NEXTflex unique dual index barcodes are designed to specifically mitigate the index hopping or spread of signal phenomenon associated with Illumina platforms that utilise a patterned flow cell. Index mis-assignment can lead to increased false positive rates, which are especially detrimental to sensitive applications. Multiplexing with NEXTflex unique dual index barcodes drastically increases processing capacity while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane.
Illumina sequencing instruments that utilise a patterned flow cell and exclusion amplification technology is known to suffer from increased levels of sample mis-assignment. The use of NEXTflex unique dual index barcodes prevents such mis-assigned reads from appearing in final data sets allowing for the highest assurance of data integrity.
Unique dual index adapters for multiplexing up to 96 Illumina sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Decreases level of mis-assigned reads in sequencing data Compatible with both paired-end and single-read Illumina sequencing
[These kits ship on dry ice.]
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex Unique Dual Index Barcodes Kit contains 96 unique dual-index barcodes, enabling users to multiplex up to 96 samples per flow cell lane. Each lot of the NEXTflex Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing. NEXTflex Unique Dual Index Barcodes are supplied in 96-well plates with each well containing 2 reactions of each barcode. These kits ship on dry ice.
Background Info:
The NEXTflex unique dual index barcodes are barcoded adapters for sequencing on Illumina platforms that provide unprecedented data security in sequencing applications. Increased mis-assignment of indexes has been shown to occur on Illumina sequencing instruments that feature a patterned flow cell and exclusion amplification technology. The NEXTflex unique dual index barcodes are designed to specifically mitigate the index hopping or spread of signal phenomenon associated with Illumina platforms that utilise a patterned flow cell. Index mis-assignment can lead to increased false positive rates, which are especially detrimental to sensitive applications. Multiplexing with NEXTflex unique dual index barcodes drastically increases processing capacity while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane.
Illumina sequencing instruments that utilise a patterned flow cell and exclusion amplification technology is known to suffer from increased levels of sample mis-assignment. The use of NEXTflex unique dual index barcodes prevents such mis-assigned reads from appearing in final data sets allowing for the highest assurance of data integrity.
Unique dual index adapters for multiplexing up to 96 Illumina sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Decreases level of mis-assigned reads in sequencing data Compatible with both paired-end and single-read Illumina sequencing
[These kits ship on dry ice.]
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex Unique Dual Index Barcodes Kit contains 96 unique dual-index barcodes, enabling users to multiplex up to 96 samples per flow cell lane. Each lot of the NEXTflex Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing. NEXTflex Unique Dual Index Barcodes are supplied in 96-well plates with each well containing 2 reactions of each barcode. These kits ship on dry ice.
Background Info:
The NEXTflex unique dual index barcodes are barcoded adapters for sequencing on Illumina platforms that provide unprecedented data security in sequencing applications. Increased mis-assignment of indexes has been shown to occur on Illumina sequencing instruments that feature a patterned flow cell and exclusion amplification technology. The NEXTflex unique dual index barcodes are designed to specifically mitigate the index hopping or spread of signal phenomenon associated with Illumina platforms that utilise a patterned flow cell. Index mis-assignment can lead to increased false positive rates, which are especially detrimental to sensitive applications. Multiplexing with NEXTflex unique dual index barcodes drastically increases processing capacity while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane.
Illumina sequencing instruments that utilise a patterned flow cell and exclusion amplification technology is known to suffer from increased levels of sample mis-assignment. The use of NEXTflex unique dual index barcodes prevents such mis-assigned reads from appearing in final data sets allowing for the highest assurance of data integrity.
Unique dual index adapters for multiplexing up to 96 Illumina sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Decreases level of mis-assigned reads in sequencing data Compatible with both paired-end and single-read Illumina sequencing
[These kits ship on dry ice.]
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex Unique Dual Index Barcodes Kit contains 96 unique dual-index barcodes, enabling users to multiplex up to 96 samples per flow cell lane. Each lot of the NEXTflex Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing. NEXTflex Unique Dual Index Barcodes are supplied in 96-well plates with each well containing 2 reactions of each barcode. These kits ship on dry ice.
Background Info:
The NEXTflex unique dual index barcodes are barcoded adapters for sequencing on Illumina platforms that provide unprecedented data security in sequencing applications. Increased mis-assignment of indexes has been shown to occur on Illumina sequencing instruments that feature a patterned flow cell and exclusion amplification technology. The NEXTflex unique dual index barcodes are designed to specifically mitigate the index hopping or spread of signal phenomenon associated with Illumina platforms that utilise a patterned flow cell. Index mis-assignment can lead to increased false positive rates, which are especially detrimental to sensitive applications. Multiplexing with NEXTflex unique dual index barcodes drastically increases processing capacity while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane.
Illumina sequencing instruments that utilise a patterned flow cell and exclusion amplification technology is known to suffer from increased levels of sample mis-assignment. The use of NEXTflex unique dual index barcodes prevents such mis-assigned reads from appearing in final data sets allowing for the highest assurance of data integrity.
Unique dual index adapters for multiplexing up to 96 Illumina sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Decreases level of mis-assigned reads in sequencing data Compatible with both paired-end and single-read Illumina sequencing
[These kits ship on dry ice.]
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex-96 DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex-96 DNA Barcodes kits accomplish this by using an indexed adapter with an 8 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. The NEXTflex-96 DNA Barcode kits are supplied in either a 96-well format (cat # 514105) or in microfuge tubes (cat # 514106). Do you want to index you samples but dont want to use 96 different barcodes? We also offer the NEXTflex DNA Barcodes in sets of 6, 12, 24 and 48 barcodes. These barcodes can be used with DNA-Seq kits, Rapid DNA-Seq kits and the Methyl-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Rapid DNA, Methyl-Seq, SeqCap EZ Target Capture, SureSelect, Cell Free DNA-Seq Kit for Illumina, NEXTflex Pre-Capture Combo, barcode blockers
Additional Info:
The NEXTflex-96 DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex-96 DNA Barcodes kits accomplish this by using an indexed adapter with an 8 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. The NEXTflex-96 DNA Barcode kits are supplied in either a 96-well format (cat # 514105) or in microfuge tubes (cat # 514106). Do you want to index you samples but dont want to use 96 different barcodes? We also offer the NEXTflex DNA Barcodes in sets of 6, 12, 24 and 48 barcodes.
These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits, other genomic DNA library prep and mRNA-Seq protocols and the NEXTflex Rapid DNA-Seq Kit.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 8 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Each kit comes with 8 reactions each of 96 multiplexed barcodes (768 reactions per kit) >>> Compatible with all DNA-Seq and mRNA-Seq application >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 1000/2000) >>> The kits are supplied in either a 96-well plate format or 96 microfuge tubes
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514105
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