The NEXTflex RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with RNA-Seq kits, directional RNA-Seq kits, Rapid RNA-Seq kits and the Rapid Directional RNA-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - RNA - Illumina Compatible
Applications:
Rapid RNA-Seq Kit, Rapid Directional RNA
Search Terms:
Rapid RNA-Seq Kit, Rapid Directional RNA-Seq
Additional Info:
The NEXTflex RNA-Seq Barcodes are adapters that were designed for use with the NEXTflex RNA-Seq Kit and the NEXTflex Directional RNA-Seq Kit (dUTP-Based). These indexes are composed of a 6 nt error-correcting sequencing offering an improved multiplex workflow and sequencing flexibility. The ability to pool samples in an efficient way significantly decreases hands on time while providing robust data quality. Their automation-friendly format enables multiplexing of up to 48 samples. The NEXTflex RNA-Seq Barcodes are available in sets of 6, 12, 24 and 48 unique adapters.
We also offer the NEXTflex-96 RNA-Seq Barcode kits, which contain 96 - 8 nt index barcodes in either a 96-well format (cat # 512915) or in microfuge tubes (cat # 512916).
BENEFITS:- >>> Index contained within adapter sequence >>> Up to 48 multiplexed samples >>> Compatible with the NEXTflex RNA-Seq Kit and NEXTflex Directional RNA-Seq Kit (dUTP-Based) >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina next-generation sequencing platforms (GAIIx, HiSeq and MiSeq)
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512912
The NEXTflex RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with RNA-Seq kits, directional RNA-Seq kits, Rapid RNA-Seq kits and the Rapid Directional RNA-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - RNA - Illumina Compatible
Applications:
Rapid RNA-Seq Kit, Rapid Directional RNA
Search Terms:
Rapid RNA-Seq Kit, Rapid Directional RNA-Seq
Additional Info:
The NEXTflex RNA-Seq Barcodes are adapters that were designed for use with the NEXTflex RNA-Seq Kit and the NEXTflex Directional RNA-Seq Kit (dUTP-Based). These indexes are composed of a 6 nt error-correcting sequencing offering an improved multiplex workflow and sequencing flexibility. The ability to pool samples in an efficient way significantly decreases hands on time while providing robust data quality. Their automation-friendly format enables multiplexing of up to 48 samples. The NEXTflex RNA-Seq Barcodes are available in sets of 6, 12, 24 and 48 unique adapters.
We also offer the NEXTflex-96 RNA-Seq Barcode kits, which contain 96 - 8 nt index barcodes in either a 96-well format (cat # 512915) or in microfuge tubes (cat # 512916).
BENEFITS:- >>> Index contained within adapter sequence >>> Up to 48 multiplexed samples >>> Compatible with the NEXTflex RNA-Seq Kit and NEXTflex Directional RNA-Seq Kit (dUTP-Based) >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina next-generation sequencing platforms (GAIIx, HiSeq and MiSeq)
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512913
The NEXTflex RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with RNA-Seq kits, directional RNA-Seq kits, Rapid RNA-Seq kits and the Rapid Directional RNA-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - RNA - Illumina Compatible
Applications:
Rapid RNA-Seq Kit, Rapid Directional RNA
Search Terms:
Rapid RNA-Seq Kit, Rapid Directional RNA-Seq
Additional Info:
The NEXTflex RNA-Seq Barcodes are adapters that were designed for use with the NEXTflex RNA-Seq Kit and the NEXTflex Directional RNA-Seq Kit (dUTP-Based). These indexes are composed of a 6 nt error-correcting sequencing offering an improved multiplex workflow and sequencing flexibility. The ability to pool samples in an efficient way significantly decreases hands on time while providing robust data quality. Their automation-friendly format enables multiplexing of up to 48 samples. The NEXTflex RNA-Seq Barcodes are available in sets of 6, 12, 24 and 48 unique adapters.
We also offer the NEXTflex-96 RNA-Seq Barcode kits, which contain 96 - 8 nt index barcodes in either a 96-well format (cat # 512915) or in microfuge tubes (cat # 512916).
BENEFITS:- >>> Index contained within adapter sequence >>> Up to 48 multiplexed samples >>> Compatible with the NEXTflex RNA-Seq Kit and NEXTflex Directional RNA-Seq Kit (dUTP-Based) >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina next-generation sequencing platforms (GAIIx, HiSeq and MiSeq)
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512914
The NEXTflex-96 RNA-Seq Barcodes Kits contain 96 unique barcodes, enabling the user to multiplex up to 96 RNA-seq samples. These kits ship on dry ice.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - RNA - Illumina Compatible
Applications:
Rapid RNA-Seq Kit, Rapid Directional RNA
Search Terms:
Rapid RNA-Seq Kit, Rapid Directional RNA-Seq
Additional Info:
The NEXTflex-96 RNA-Seq Barcodes are adapters that were designed for use with the NEXTflex RNA-Seq Kit and the NEXTflex Directional RNA-Seq Kit (dUTP-Based).
The NEXTflex-96 RNA-Seq Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex-96 RNA-Seq Barcodes kits accomplish this by using an indexed adapter with an 8 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. The NEXTflex-96 RNA-Seq Barcode kits are supplied in either a 96-well format (cat# 512915) or in microfuge tubes (cat# 512016).
Do you want to index you samples but dont want to use 96 different barcodes? We also offer the NEXTflex RNA-Seq Barcodes in sets of 6, 12, 24 and 48 barcodes.
BENEFITS:- >>> Index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Up to 96 multiplexed samples >>> Compatible with the NEXTflex RNA-Seq Kit and NEXTflex Directional RNA-Seq Kit (dUTP-Based) >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina next-generation sequencing platforms (GAIIx, HiSeq and MiSeq)
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512915
The NEXTflex RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with RNA-Seq kits, directional RNA-Seq kits, Rapid RNA-Seq kits and the Rapid Directional RNA-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex RNA-Seq 2.0 UDI Barcodes are adapters designed for use with the NEXTflex Rapid Directional RNA-seq Kit 2.0.
The NEXTFLEX® Unique Dual Index Barcodes utilize two unique 8 nt indexes on each UDI adapter. The 8 nt indices are designed with a Hamming Distance of at least three throughout the entire series, permitting error correction. Each NEXTFLEX® Unique Dual Index Barcode comprises the entire flow cell binding site, sequencing primer binding site, and index sequences, making PCR enrichment optional.
Patterned flow cells, such as those found on the NovaSeq® and HiSeq® 3000/4000 platforms, have been recognized to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity.
Barcode sets available: NOVA-512920-eval16 NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-8 in tubes) NOVA-512920-eval48A NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-24 in tubes) NOVA-512920-eval48B NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 24-48 in tubes) NOVA-512920 NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-96 in a plate) with custom sets available up to 1920 barcoded adapters.
BENEFITS:- >>> Unique dual index adapters for multiplexing up to 384 Illumina® sequencing libraries >>> Mitigate index hopping or spread of signal that can occur during sequencing >>> Each lot of the NEXTFLEX® Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing >>> Compatible with both paired-end and single-read Illumina® sequencing
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512913
The NEXTflex RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with RNA-Seq kits, directional RNA-Seq kits, Rapid RNA-Seq kits and the Rapid Directional RNA-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex RNA-Seq 2.0 UDI Barcodes are adapters designed for use with the NEXTflex Rapid Directional RNA-seq Kit 2.0.
The NEXTFLEX® Unique Dual Index Barcodes utilize two unique 8 nt indexes on each UDI adapter. The 8 nt indices are designed with a Hamming Distance of at least three throughout the entire series, permitting error correction. Each NEXTFLEX® Unique Dual Index Barcode comprises the entire flow cell binding site, sequencing primer binding site, and index sequences, making PCR enrichment optional.
Patterned flow cells, such as those found on the NovaSeq® and HiSeq® 3000/4000 platforms, have been recognized to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity.
Barcode sets available: NOVA-512920-eval16 NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-8 in tubes) NOVA-512920-eval48A NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-24 in tubes) NOVA-512920-eval48B NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 24-48 in tubes) NOVA-512920 NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-96 in a plate) with custom sets available up to 1920 barcoded adapters.
BENEFITS:- >>> Unique dual index adapters for multiplexing up to 384 Illumina® sequencing libraries >>> Mitigate index hopping or spread of signal that can occur during sequencing >>> Each lot of the NEXTFLEX® Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing >>> Compatible with both paired-end and single-read Illumina® sequencing
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512914
The NEXTflex RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with RNA-Seq kits, directional RNA-Seq kits, Rapid RNA-Seq kits and the Rapid Directional RNA-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex RNA-Seq 2.0 UDI Barcodes are adapters designed for use with the NEXTflex Rapid Directional RNA-seq Kit 2.0.
The NEXTFLEX® Unique Dual Index Barcodes utilize two unique 8 nt indexes on each UDI adapter. The 8 nt indices are designed with a Hamming Distance of at least three throughout the entire series, permitting error correction. Each NEXTFLEX® Unique Dual Index Barcode comprises the entire flow cell binding site, sequencing primer binding site, and index sequences, making PCR enrichment optional.
Patterned flow cells, such as those found on the NovaSeq® and HiSeq® 3000/4000 platforms, have been recognized to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity.
Barcode sets available: NOVA-512920-eval16 NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-8 in tubes) NOVA-512920-eval48A NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-24 in tubes) NOVA-512920-eval48B NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 24-48 in tubes) NOVA-512920 NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-96 in a plate) with custom sets available up to 1920 barcoded adapters.
BENEFITS:- >>> Unique dual index adapters for multiplexing up to 384 Illumina® sequencing libraries >>> Mitigate index hopping or spread of signal that can occur during sequencing >>> Each lot of the NEXTFLEX® Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing >>> Compatible with both paired-end and single-read Illumina® sequencing
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512914
The NEXTflex RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with RNA-Seq kits, directional RNA-Seq kits, Rapid RNA-Seq kits and the Rapid Directional RNA-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex RNA-Seq 2.0 UDI Barcodes are adapters designed for use with the NEXTflex Rapid Directional RNA-seq Kit 2.0.
The NEXTFLEX® Unique Dual Index Barcodes utilize two unique 8 nt indexes on each UDI adapter. The 8 nt indices are designed with a Hamming Distance of at least three throughout the entire series, permitting error correction. Each NEXTFLEX® Unique Dual Index Barcode comprises the entire flow cell binding site, sequencing primer binding site, and index sequences, making PCR enrichment optional.
Patterned flow cells, such as those found on the NovaSeq® and HiSeq® 3000/4000 platforms, have been recognized to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity.
Barcode sets available: NOVA-512920-eval16 NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-8 in tubes) NOVA-512920-eval48A NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-24 in tubes) NOVA-512920-eval48B NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 24-48 in tubes) NOVA-512920 NEXTflex RNA-Seq 2.0 UDI barcodes (Barcodes 1-96 in a plate) with custom sets available up to 1920 barcoded adapters.
BENEFITS:- >>> Unique dual index adapters for multiplexing up to 384 Illumina® sequencing libraries >>> Mitigate index hopping or spread of signal that can occur during sequencing >>> Each lot of the NEXTFLEX® Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing >>> Compatible with both paired-end and single-read Illumina® sequencing
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512914
The NEXTflex Poly(A) Beads (magnetic separation beads) provide a convenient method for batch purification of pure, intact mRNA upstream of library preparation for RNA-Seq. NEXTflex Poly(A) Beads beads use oligo(dT) primer to isolate polyadenylated messenger RNAs from 1 µg 10 µg of previously isolated total RNA. Separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. Ease of use and consistent results makes the NEXTflex Poly(A) Beads ideal for NGS library preparation applications.
These beads have been validated with the following kits:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 1 - 10 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512979
The NEXTflex Poly(A) Beads (magnetic separation beads) provide a convenient method for batch purification of pure, intact mRNA upstream of library preparation for RNA-Seq. NEXTflex Poly(A) Beads beads use oligo(dT) primer to isolate polyadenylated messenger RNAs from 1 µg 10 µg of previously isolated total RNA. Separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. Ease of use and consistent results makes the NEXTflex Poly(A) Beads ideal for NGS library preparation applications.
These beads have been validated with the following kits:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 1 - 10 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512980
The NEXTflex Poly(A) Beads (magnetic separation beads) provide a convenient method for batch purification of pure, intact mRNA upstream of library preparation for RNA-Seq. NEXTflex Poly(A) Beads beads use oligo(dT) primer to isolate polyadenylated messenger RNAs from 1 µg 10 µg of previously isolated total RNA. Separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. Ease of use and consistent results makes the NEXTflex Poly(A) Beads ideal for NGS library preparation applications.
These beads have been validated with the following kits:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 1 - 10 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512981
Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples intended for use to produce libraries for NGS RNA-Seq experiments. Removal of rRNA avoids the waste of reagents and bioinformatic resources to sequence and align the ~85% of total RNA comprised of rRNA, which is generally not a target of interest in NGS sequencing experiments.
The NEXTFLEX® Poly(A) Beads 2.0 offer improved mRNA yields, lower rRNA contamination, and a more simplified protocol, verified from 10 ng - 5 µg of total RNA for a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep.
Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. These beads have been validated with the new and improved NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0.
The NEXTFLEX® Poly(A) beads 2.0 are an easy and cost-effective method for removing rRNA in RNA-Seq libraries. This product takes advantage of the tract of adenosine residues present at the 3 ends of the vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines (oligo dT) immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.
The NEXTFLEX® Poly(A) Beads 2.0 contains enough material to extract mRNA from 8, 48 or 96 total RNA samples for subsequent RNA-seq library prep or other applications. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at 4°C.
These beads have been validated with the following kits: NEXTflex Directional RNA-Seq Kit NEXTflex Directional RNA-Seq Kit 2.0
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples intended for use to produce libraries for NGS RNA-Seq experiments. Removal of rRNA avoids the waste of reagents and bioinformatic resources to sequence and align the ~85% of total RNA comprised of rRNA, which is generally not a target of interest in NGS sequencing experiments.
The NEXTFLEX® Poly(A) Beads 2.0 offer improved mRNA yields, lower rRNA contamination, and a more simplified protocol, verified from 10 ng - 5 µg of total RNA for a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep.
Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. These beads have been validated with the new and improved NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0.
The NEXTFLEX® Poly(A) beads 2.0 are an easy and cost-effective method for removing rRNA in RNA-Seq libraries. This product takes advantage of the tract of adenosine residues present at the 3 ends of the vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines (oligo dT) immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.
The NEXTFLEX® Poly(A) Beads 2.0 contains enough material to extract mRNA from 8, 48 or 96 total RNA samples for subsequent RNA-seq library prep or other applications. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at 4°C.
These beads have been validated with the following kits: NEXTflex Directional RNA-Seq Kit NEXTflex Directional RNA-Seq Kit 2.0
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples intended for use to produce libraries for NGS RNA-Seq experiments. Removal of rRNA avoids the waste of reagents and bioinformatic resources to sequence and align the ~85% of total RNA comprised of rRNA, which is generally not a target of interest in NGS sequencing experiments.
The NEXTFLEX® Poly(A) Beads 2.0 offer improved mRNA yields, lower rRNA contamination, and a more simplified protocol, verified from 10 ng - 5 µg of total RNA for a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep.
Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. These beads have been validated with the new and improved NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0.
The NEXTFLEX® Poly(A) beads 2.0 are an easy and cost-effective method for removing rRNA in RNA-Seq libraries. This product takes advantage of the tract of adenosine residues present at the 3 ends of the vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines (oligo dT) immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.
The NEXTFLEX® Poly(A) Beads 2.0 contains enough material to extract mRNA from 8, 48 or 96 total RNA samples for subsequent RNA-seq library prep or other applications. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at 4°C.
These beads have been validated with the following kits: NEXTflex Directional RNA-Seq Kit NEXTflex Directional RNA-Seq Kit 2.0
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
The NEXTflex Rapid RNA-Seq Kit contains enough material to prepare 8 or 48 RNA samples for Illumina compatible sequencing. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The patent pending NEXTflex Rapid RNA-Seq Kits provide an easy and flexible solution for generating single, paired-end and multiplexed libraries from mRNA or rRNA depleted total RNA. Using the NEXTflex RNA-Seq library prep protocol, mRNA or rRNA depleted RNA is fragmented using a cationic buffer. Fragmented RNA undergoes first and second strand synthesis, followed by end-repair, adenylation, ligation and PCR.
This kit incorporates the NEXTflex Rapid Reverse Transcriptase, a robust, thermostable RT that outperforms any other RT on the market for NGS applications.
MULTIPLEXING This kit was designed to be used in conjunction with the NEXTflex RNA-Seq or NEXTflex-96 RNA-Seq Barcodes for multiplexing. The availability of up to 96 unique adapter barcodes makes this the most high-throughput kit available for RNA-Seq.
BENEFITS:- >>> Faster than traditional RNA-seq protocols >>> Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads >>> Input - 10 - 100 ng of mRNA or rRNA depleted total RNA >>> Up to 96 barcodes available for multiplexing contain embedded index sequence >>> Automation-friendly workflow is compatible with liquid handlers >>> Functionally validated with Illumina sequencing platforms
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5138-01
The NEXTflex Rapid RNA-Seq Kit contains enough material to prepare 8 or 48 RNA samples for Illumina compatible sequencing. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The patent pending NEXTflex Rapid RNA-Seq Kits provide an easy and flexible solution for generating single, paired-end and multiplexed libraries from mRNA or rRNA depleted total RNA. Using the NEXTflex RNA-Seq library prep protocol, mRNA or rRNA depleted RNA is fragmented using a cationic buffer. Fragmented RNA undergoes first and second strand synthesis, followed by end-repair, adenylation, ligation and PCR.
This kit incorporates the NEXTflex Rapid Reverse Transcriptase, a robust, thermostable RT that outperforms any other RT on the market for NGS applications.
MULTIPLEXING This kit was designed to be used in conjunction with the NEXTflex RNA-Seq or NEXTflex-96 RNA-Seq Barcodes for multiplexing. The availability of up to 96 unique adapter barcodes makes this the most high-throughput kit available for RNA-Seq.
BENEFITS:- >>> Faster than traditional RNA-seq protocols >>> Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads >>> Input - 10 - 100 ng of mRNA or rRNA depleted total RNA >>> Up to 96 barcodes available for multiplexing contain embedded index sequence >>> Automation-friendly workflow is compatible with liquid handlers >>> Functionally validated with Illumina sequencing platforms
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5138-02
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