The NEXTflex DNA Barcodes Kits contains 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with DNA-Seq kits, Rapid DNA-Seq kits and the Methyl-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Rapid DNA, Methyl-Seq, SeqCap EZ Target Capture, SureSelect, Cell Free DNA-Seq Kit for Illumina, barcode blockers
Additional Info:
NEXTflex DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits and PCR-free sequencing workflows and the NEXTflex Rapid DNA-Seq Kit.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 6 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Up to 48 multiplexed samples (up to 384 reactions per kit) >>> Compatible with PCR-free sequencing workflows >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514101
The NEXTflex DNA Barcodes Kits contains 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with DNA-Seq kits, Rapid DNA-Seq kits and the Methyl-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Rapid DNA, Methyl-Seq, SeqCap EZ Target Capture, SureSelect, Cell Free DNA-Seq Kit for Illumina, barcode blockers
Additional Info:
NEXTflex DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits and PCR-free sequencing workflows and the NEXTflex Rapid DNA-Seq Kit.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 6 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Up to 48 multiplexed samples (up to 384 reactions per kit) >>> Compatible with PCR-free sequencing workflows >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514102
The NEXTflex DNA Barcodes Kits contains 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with DNA-Seq kits, Rapid DNA-Seq kits and the Methyl-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Rapid DNA, Methyl-Seq, SeqCap EZ Target Capture, SureSelect, Cell Free DNA-Seq Kit for Illumina, barcode blockers
Additional Info:
NEXTflex DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits and PCR-free sequencing workflows and the NEXTflex Rapid DNA-Seq Kit.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 6 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Up to 48 multiplexed samples (up to 384 reactions per kit) >>> Compatible with PCR-free sequencing workflows >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514103
The NEXTflex DNA Barcodes Kits contains 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with DNA-Seq kits, Rapid DNA-Seq kits and the Methyl-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Rapid DNA, Methyl-Seq, SeqCap EZ Target Capture, SureSelect, Cell Free DNA-Seq Kit for Illumina, barcode blockers
Additional Info:
NEXTflex DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex DNA Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits and PCR-free sequencing workflows and the NEXTflex Rapid DNA-Seq Kit.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 6 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Up to 48 multiplexed samples (up to 384 reactions per kit) >>> Compatible with PCR-free sequencing workflows >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 2000)
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514104
The NEXTflex-96 DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex-96 DNA Barcodes kits accomplish this by using an indexed adapter with an 8 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. The NEXTflex-96 DNA Barcode kits are supplied in either a 96-well format (cat # 514105) or in microfuge tubes (cat # 514106). Do you want to index you samples but dont want to use 96 different barcodes? We also offer the NEXTflex DNA Barcodes in sets of 6, 12, 24 and 48 barcodes. These barcodes can be used with DNA-Seq kits, Rapid DNA-Seq kits and the Methyl-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Rapid DNA, Methyl-Seq, SeqCap EZ Target Capture, SureSelect, Cell Free DNA-Seq Kit for Illumina, NEXTflex Pre-Capture Combo, barcode blockers
Additional Info:
The NEXTflex-96 DNA Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTflex-96 DNA Barcodes kits accomplish this by using an indexed adapter with an 8 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR. The NEXTflex index is contained within the adapter sequence eliminating the need to perform PCR to add flow cell binding sequences. The NEXTflex-96 DNA Barcode kits are supplied in either a 96-well format (cat # 514105) or in microfuge tubes (cat # 514106). Do you want to index you samples but dont want to use 96 different barcodes? We also offer the NEXTflex DNA Barcodes in sets of 6, 12, 24 and 48 barcodes.
These barcodes can be used with single, paired-end and multiplex reads and are compatible with the NEXTflex DNA Sequencing kits, other genomic DNA library prep and mRNA-Seq protocols and the NEXTflex Rapid DNA-Seq Kit.
For larger volume requirements, customized and bulk packaging is available.
[BENEFITS] >>> 8 nt index contained within adapter sequence eliminates the need to perform PCR to add flow cell binding sequences >>> Each kit comes with 8 reactions each of 96 multiplexed barcodes (768 reactions per kit) >>> Compatible with all DNA-Seq and mRNA-Seq application >>> Considerably reduce your per-sample sequencing cost by barcoded multiplexing >>> Increase your sequencing scale by pooling 100s of samples on a single flow cell >>> Compatible with Illumina Next-Generation Sequencing platforms (GAII and HiSeq 1000/2000) >>> The kits are supplied in either a 96-well plate format or 96 microfuge tubes
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514105
The NEXTflex DNA Barcode Blockers for SeqCap are designed for use with the NEXTflex DNA Barcodes. Both are compatible with Roche NimbleGens SeqCap EZ library target capture systems. The NEXTflex DNA Barcode Blockers for SeqCap are available in sets of 6, 12, 24 or 96 blocking oligos.
Alternatively, a complete library, barcode and blocker solution is available. The NEXTflex Pre-Capture Combo Kit (NimbleGen SeqCap v3) includes NEXTflex DNA library prep, NEXTflex DNA barcodes for multiplexing and barcode blockers.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
SeqCap EZ Target Capture, SureSelect,Nimblegen, barcode blockers
Additional Info:
The NEXTflex DNA Barcode Blockers for SeqCap contain 6, 12, 24, or 96 oligo blockers designed to be used in conjunction with NEXTflex DNA Barcodes.
>>> Multiplex Compatible 96 barcodes and barcode blockers available >>> Compatible with NEXTflex DNA Barcodes >>> Validated in combination with Roche NimbleGens SeqCap EZ library target capture kits and Illumina sequencing >>> Simplifies and reduces the cost of NimbleGens SeqCap EZ library target capture [These kits ship on dry ice.]
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514134
The NEXTFLEX® Universal Blockers are designed to prevent concatemerisation of library molecules during target capture hybridisations via the adapter sequences that are added to all library molecules before enrichment. Each strand of these adapter sequences can hybridise with the complementary adapter sequence strand of another library molecule during this enrichment hybridisation, creating a "daisy-chain" effect between off-target library molecules and the on-target library molecules that are the intended targets of the capture probe set used in the enrichment hybridization. By blocking this complementary adapter sequence interaction, the number of on-target reads improves dramatically and the depth of enrichment increases.
The NEXTFLEX® Universal Blockers are designed for use with Illumina® indexed adapters. They are effective in blocking single-indexed or dual-indexed libraries, without being affected by index length or the presence of a unique molecular index (UMI). The NEXTFLEX® Universal Blockers are designed to target both strands of the adapter sequence regions.
The NEXTFLEX® Universal Blockers are compatible with ligation-based workflows, such as the libraries prepared with NEXTFLEX® DNA Barcodes, NEXTFLEX-96 DNA Barcodes, NEXTFLEX HT Barcodes, NEXTFLEX® Dual-Index Barcodes and NEXTFLEX® Unique Dual Index Barcodes. They are also compatible with tagmentation-based workflows, such as Illumina® Nextera library prep kits.
The manual for the kit provides details of how to use the Universal blockers with each type of major hybridisation capture library.
NEXTFLEX® Universal Blockers
Effective in blocking single-index or dual-index libraries Simplifies and reduces the cost of target capture without sacrificing performance 96 barcode blockers available Compatible with all NEXTFLEX® barcodes and all other ligation-based workflows for Illumina® sequencing
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated.
The NEXTFLEX® Universal Blockers are designed to prevent concatemerisation of library molecules during target capture hybridisations via the adapter sequences that are added to all library molecules before enrichment. Each strand of these adapter sequences can hybridise with the complementary adapter sequence strand of another library molecule during this enrichment hybridisation, creating a "daisy-chain" effect between off-target library molecules and the on-target library molecules that are the intended targets of the capture probe set used in the enrichment hybridization. By blocking this complementary adapter sequence interaction, the number of on-target reads improves dramatically and the depth of enrichment increases.
The NEXTFLEX® Universal Blockers are designed for use with Illumina® indexed adapters. They are effective in blocking single-indexed or dual-indexed libraries, without being affected by index length or the presence of a unique molecular index (UMI). The NEXTFLEX® Universal Blockers are designed to target both strands of the adapter sequence regions.
The NEXTFLEX® Universal Blockers are compatible with ligation-based workflows, such as the libraries prepared with NEXTFLEX® DNA Barcodes, NEXTFLEX-96 DNA Barcodes, NEXTFLEX HT Barcodes, NEXTFLEX® Dual-Index Barcodes and NEXTFLEX® Unique Dual Index Barcodes. They are also compatible with tagmentation-based workflows, such as Illumina® Nextera library prep kits.
The manual for the kit provides details of how to use the Universal blockers with each type of major hybridisation capture library.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated.
The NEXTFLEX® Universal Blockers are designed to prevent concatemerisation of library molecules during target capture hybridisations via the adapter sequences that are added to all library molecules before enrichment. Each strand of these adapter sequences can hybridise with the complementary adapter sequence strand of another library molecule during this enrichment hybridisation, creating a "daisy-chain" effect between off-target library molecules and the on-target library molecules that are the intended targets of the capture probe set used in the enrichment hybridization. By blocking this complementary adapter sequence interaction, the number of on-target reads improves dramatically and the depth of enrichment increases.
The NEXTFLEX® Universal Blockers are designed for use with Illumina® indexed adapters. They are effective in blocking single-indexed or dual-indexed libraries, without being affected by index length or the presence of a unique molecular index (UMI). The NEXTFLEX® Universal Blockers are designed to target both strands of the adapter sequence regions.
The NEXTFLEX® Universal Blockers are compatible with ligation-based workflows, such as the libraries prepared with NEXTFLEX® DNA Barcodes, NEXTFLEX-96 DNA Barcodes, NEXTFLEX HT Barcodes, NEXTFLEX® Dual-Index Barcodes and NEXTFLEX® Unique Dual Index Barcodes. They are also compatible with tagmentation-based workflows, such as Illumina® Nextera library prep kits.
The manual for the kit provides details of how to use the Universal blockers with each type of major hybridisation capture library.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated.
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