Ultra-fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification.
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) analyze 1-5 targets in 1 reaction mix with dUTP and UNG available to prevent carry-over contamination compatible with most cyclers different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start, and faster extension rates compared to the wild-type Taq DNA polymerase. qPCR buffer: Includes 15 mM MgCl2 (1x PCR solution 3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), additives that maximize efficiency of PCR Reference dye: ROX dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR
Ultra fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification. Suitable for UNG treatment to prevent cross-contamination.
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) analyze 1-5 targets in 1 reaction mix with dUTP and UNG available to prevent carry-over contamination compatible with most cyclers different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start, and faster extension rates compared to the wild-type Taq DNA polymerase. qPCR buffer: Includes 16.5 mM MgCl2 - 1x PCR solution 3.3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG and additives that maximize efficiency of PCR Reference dye: Purple dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
Ultra-fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification.
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) analyze 1-5 targets in 1 reaction mix with dUTP and UNG available to prevent carry-over contamination compatible with most cyclers different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
ROX dye: If ROX, Texas Red or similar dye is used as one of the fluorophores, ROX passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available.
Ultra fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification. Suitable for UNG treatment to prevent cross-contamination.
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) analyze 1-5 targets in 1 reaction mix with dUTP and UNG available to prevent carry-over contamination compatible with most cyclers different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase. qPCR buffer: Includes 16.5 mM MgCl2 (1x PCR solution 3.3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
Purple dye: If Cy5 or similar dye is used as one of the fluorophores, Purple passive reference dye might interfere with the signal. A version without passive reference or with ROX reference dye is available
Ultra fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification. Suitable for UNG treatment to prevent cross-contamination.
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) analyze 1-5 targets in 1 reaction mix with dUTP and UNG available to prevent carry-over contamination compatible with most cyclers different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start, and faster extension rates compared to the wild-type Taq DNA polymerase.
qPCR buffer: Includes 16.5 mM MgCl2 (1x PCR solution 3.3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR
Ultra-fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification.
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) analyze 1-5 targets in 1 reaction mix with dUTP and UNG available to prevent carry-over contamination compatible with most cyclers different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase. qPCR buffer: Includes 15 mM MgCl2 (1x PCR solution 3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), additives that maximize efficiency of PCR
Robust and reliable hot-start Master Mix with higher fidelity and longer amplification range for more demanding reactions.
Background Info:
A ready-to-use 5X-concentrated solution for more demanding endpoint PCR assays. In addition to the hot-start Taq polymerase HOT FIREPol® this master mix contains a proofreading enzyme which offers enhanced fidelity and performance. The mix has regular and Ready-to-Load version and different MgCl? options for easier optimization.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
increased yield, sensitivity and specificity up to 5x higher fidelity suitable for templates up to 5 kb reduced primer dimer formation Ready-to-Load mix available reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start Proofreading enzyme: to enhance fidelity 5x Blend Master Mix Buffer with 12. 5 mM MgCl2 1x PCR solution 1.5 mM MgCl2 1 mM dNTPs of each: 1x PCR solution 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP Bovine serum albumin (BSA) to enhance PCR reaction
Robust and reliable hot-start Master Mix with higher fidelity and longer amplification range for more demanding reactions.
Background Info:
A ready-to-use 5X-concentrated solution for more demanding endpoint PCR assays. In addition to the hot-start Taq polymerase HOT FIREPol® this master mix contains a proofreading enzyme which offers enhanced fidelity and performance. The mix has regular and Ready-to-Load version and different MgCl? options for easier optimization.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
increased yield, sensitivity and specificity up to 5x higher fidelity suitable for templates up to 5 kb reduced primer dimer formation Ready-to-Load mix available reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start Proofreading enzyme to enhance fidelity 5x Blend Master Mix Buffer with 7,5 mM MgCl2: 1x PCR solution 1,5 mM MgCl2 1 mM dNTPs of each: 1x PCR solution 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP Bovine serum albumine (BSA) to enhance PCR reaction
Robust and reliable hot-start Master Mix with higher fidelity and longer amplification range for more demanding reactions.
Background Info:
A ready-to-use 5X-concentrated solution for more demanding endpoint PCR assays. In addition to the hot-start Taq polymerase HOT FIREPol® this master mix contains a proofreading enzyme which offers enhanced fidelity and performance. The mix has regular and Ready-to-Load version and different MgCl? options for easier optimization.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
increased yield, sensitivity and specificity up to 5x higher fidelity suitable for templates up to 5 kb reduced primer dimer formation Ready-to-Load mix available reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start Proofreading enzyme: to enhance fidelity 5x Blend Master Mix Buffer with 10 mM MgCl2: 1x PCR solution 2 mM MgCl2 1 mM dNTPs of each: 1x PCR solution 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP Bovine serum albumin (BSA) to enhance PCR reaction
Extremely fast and highly sensitive dye-based qPCR that includes novel in-silico designed SolisFAST® DNA Polymerase Mix for most demanding applications
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using SolisGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases. It has two versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low ROX level for signal normalization. SolisGreen® qPCR mixes are suitable for commercial and diagnostic applications and require no additional licensing.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) bright and sensitive SolisGreen® dye suitable for commercial applications compatible with most cyclers, except high ROX different product versions: no ROX and ROX reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.
qPCR buffer: Includes 7.5 mM MgCl2 (1X PCR solution 1.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), stabilizers and enhancers that maximize efficiency of PCR reaction
Reference dye: ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR
Fluorescent dye: SolisGreen® is a nucleic acid dye detected in the FAM or SYBR® Green I channel
Extremely fast and highly sensitive dye-based qPCR that includes novel in-silico designed SolisFAST® DNA Polymerase Mix for most demanding applications
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using SolisGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases. It has two versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low ROX level for signal normalization. SolisGreen® qPCR mixes are suitable for commercial and diagnostic applications and require no additional licensing.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) bright and sensitive SolisGreen® dye suitable for commercial applications compatible with most cyclers, except high ROX different product versions: no ROX and ROX reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.
qPCR buffer: Includes 7.5 mM MgCl2 (1X PCR solution 1.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), stabilizers and enhancers that maximize efficiency of PCR reaction
Fluorescent dye: SolisGreen® is a nucleic acid dye detected in the FAM or SYBR® Green I channel
A chemically modified hot-start version of the thermostable Taq DNA polymerase FIREPol®. This enzyme is activated only by pre-incubation at 95°C, preventing any unspecific polymerase activity at lower temperatures during reaction set-up.
Background Info:
Chemically modified hot-start version of the thermostable Taq DNA polymerase FIREPol®. This enzyme is activated only after heat treatment at 95°C which prevents any unspecific polymerase activity at lower temperatures during reaction set-up. HOT FIREPol® DNA polymerase is supplied with 2 reaction buffers, 25 mM MgCl? and an additive for difficult templates. HOT FIREPol® 10x Buffer B2 contains non-ionic detergent suppressing inhibitory effects of the trace of DNA extraction buffers and enhancing PCR yield and efficiency.
Product Type:
Endpoint PCR
Format:
Product kit
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
increased specificity and sensitivity reduced primer dimer formation suitable for TA cloning reaction buffer with and without detergent included Solution S included in a separate vial for GC-rich templates MgCl? included in a separate vial reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase (5 U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers. HOT FIREPol® 10x Buffer B1 (without Mg2+ and detergent): 0.7 M Tris-HCl, 0.175 M (NH4)2SO4. HOT FIREPol® 10x Buffer B2 (without Mg2+): 0.7 M Tris-HCl, 0.175 M (NH4)2SO4, 0.2% w/v Tween-20. HOT FIREPol® 10x Buffer B2 contains non-ionic detergent suppressing inhibitory effects of the trace of DNA extraction buffers and enhancing PCR yield and efficiency. 25 mM MgCl2 10x Solution S is an additive that facilitates the amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined final concentration (1x, 2x or 3x solution). 10x Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplification occurs.
Vials included in the kit:
HOT FIREPol® DNA Polymerase 1x500 U | 100 µl 10x Solution S 1x0.1 ml 25 mM MgCl2 1x2.5 ml HOT FIREPol® 10x Buffer B1 1x2.5 ml HOT FIREPol® 10x Buffer B2 1x2.5 ml
Robust and reliable hot-start Master Mix with higher fidelity and longer amplification range for more demanding reactions. Includes two tracking dyes.
Background Info:
A ready-to-use 5X-concentrated solution for more demanding endpoint PCR assays. In addition to the hot-start Taq polymerase HOT FIREPol® this master mix contains a proofreading enzyme which offers enhanced fidelity and performance. The mix has regular and Ready-to-Load version and different MgCl? options for easier optimization. Ready-to-Load mix ncludes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
increased yield, sensitivity and specificity up to 5x higher fidelity suitable for templates up to 5 kb reduced primer dimer formation Ready-to-Load mix available reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start Proofreading enzyme: to enhance fidelity 5x Blend Master Mix Buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 1 mM dNTPs of each 1x PCR solution 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP Bovine serum albumine (BSA) to enhance PCR reaction Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment Yellow dye: migration rate in excess of primers in 1% agarose gel: <35-45 bp Compound that increases sample density for direct loading
Robust and reliable hot-start Master Mix with higher fidelity and longer amplification range for more demanding reactions. Includes two tracking dyes.
Background Info:
A ready-to-use 5X-concentrated solution for more demanding endpoint PCR assays. In addition to the hot-start Taq polymerase HOT FIREPol® this master mix contains a proofreading enzyme which offers enhanced fidelity and performance. The mix has regular and Ready-to-Load version and different MgCl? options for easier optimization. Ready-to-Load mix ncludes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
increased yield, sensitivity and specificity up to 5x higher fidelity suitable for templates up to 5 kb reduced primer dimer formation Ready-to-Load mix available reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start Proofreading enzyme: to enhance fidelity 5x Blend Master Mix Buffer with 10 mM MgCl2: 1x PCR solution 2 mM MgCl2 1 mM dNTPs of each: 1x PCR solution 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP Bovine serum albumin (BSA) to enhance PCR reaction Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment Yellow dye: migration rate in excess of primers in 1% agarose gel: <35-45 bp Compound that increases sample density for direct loading
Hot-start Master Mix designed to provide highly specific high-yield amplification of GC-rich templates.
Background Info:
A ready-to-use 5X-concentrated solution for endpoint PCR assays, optimised for GC-rich templates. It contains a hot-start Taq DNA polymerase HOT FIREPol® and allows amplification of up to 5 kb DNA templates. Separate tubes of 25 mM MgCl? and an additive for difficult templates (100% DMSO) are included with the mix.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
excellent amplification with templates up to 79% GC content suitable for templates up to 5 kb vials of 100% DMSO and 25 mM MgCl? enable flexibility in reaction optimization reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x HOT FIREPol® GC Buffer with 7.5 mM MgCl2: 1x PCR solution 1.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP Bovine serum albumin (BSA) to enhance PCR reaction In separate vials: 100% DMSO is included in the kit in a separate vial. DMSO is recommended as a PCR additive for templates with high GC content. In some cases, DMSO is also required to relax secondary structures. 25 mM MgCl2 for further optimization
Vials included in the kit:
HOT FIREPol® GC Master Mix 1x1 ml | 250 rxn 25 mM MgCl2 1x0.5 ml DMSO 100% 1x0.5 ml
Robust and reliable hot-start Master Mix with higher fidelity and longer amplification range for more demanding reactions. Includes two tracking dyes.
Background Info:
A ready-to-use 5X-concentrated solution for more demanding endpoint PCR assays. In addition to the hot-start Taq polymerase HOT FIREPol® this master mix contains a proofreading enzyme which offers enhanced fidelity and performance. The mix has regular and Ready-to-Load version and different MgCl? options for easier optimization. Ready-to-Load mix ncludes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
increased yield, sensitivity and specificity up to 5x higher fidelity suitable for templates up to 5 kb reduced primer dimer formation Ready-to-Load mix available reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start Proofreading enzyme: to enhance fidelity 5x Blend Master Mix Buffer with 7. 5 mM MgCl2: 1x PCR solution 1.5 mM MgCl2 1 mM dNTPs of each: 1x PCR solution 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP Bovine serum albumine (BSA) to enhance PCR reaction Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment Yellow dye: migration rate in excess of primers in 1% agarose gel: <35-45 bp Compound that increases sample density for direct loading
FIREPol® is a highly processive, thermostable Taq DNA polymerase with unique 30-day stability at room temperature.
Background Info:
A genetically modified thermostable Taq DNA polymerase that provides robust and reproducible results. FIREPol® DNA polymerase is supplied with 2 reaction buffers, 25 mM MgCl2 and an additive for difficult templates. FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency.
Product Type:
Endpoint PCR
Format:
Product kit
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
robust amplification for routine applications suitable for templates up to 5 kb suitable for TA cloning reaction buffers with and without detergent included Solution S included in a separate vial for GC-rich templates MgCl2 included in a separate vial reaction set-up and shipment without ice
Mix/Kit Components:
FIREPol® DNA Polymerase (5 U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers. FIREPol® 10x Buffer B (without Mg2+ ): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20. FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency. FIREPol® 10x Buffer BD (without Mg2+ and detergent): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4. 25 mM MgCl2 10x Solution S is an additive that facilitates the amplification of difficult templates (e.g. GC-rich DNA templates).
Vials included in the kit:
FIREPol® DNA Polymerase 1x500 U | 100 µl 10x Reaction Buffer B 1x2.5 ml 10x Reaction Buffer BD 1x2.5 ml 10x Solution S 1x0.1 ml 25 mM MgCl2 1x2.5 ml
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for singleplex and multiplex endpoint PCR assays. It contains a hot-start Taq DNA polymerase HOT FIREPol® and allows amplification of up to 5 kb DNA templates. The mix has two versions - regular and Ready-to-Load mix which includes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
sensitive detection of up to 18 targets per reaction suitable for templates up to 5 kb increased sensitivity and yield reduced primer dimer formation Ready-to-Load mix available reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase 5x Multiplex Buffer with 10 mM MgCl2 (1x PCR solution 2 mM MgCl2) dNTPs BSA Compound that increases sample density for direct loading (loading dyes not included)
FIREPol® is a highly processive, thermostable Taq DNA polymerase with unique 30-day stability at room temperature.
Background Info:
A genetically modified thermostable Taq DNA polymerase that provides robust and reproducible results. FIREPol® DNA polymerase is supplied with 2 reaction buffers, 25 mM MgCl2 and an additive for difficult templates. FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency.
Product Type:
Endpoint PCR
Format:
Product kit
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
robust amplification for routine applications suitable for templates up to 5 kb suitable for TA cloning reaction buffers with and without detergent included Solution S included in a separate vial for GC-rich templates MgCl2 included in a separate vial reaction set-up and shipment without ice
Mix/Kit Components:
FIREPol® DNA Polymerase (5 U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers. FIREPol® 10x Buffer B (without Mg2+ ): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20. FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency. FIREPol® 10x Buffer BD (without Mg2+ and detergent): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4. 25 mM MgCl2 10x Solution S is an additive that facilitates the amplification of difficult templates (e.g. GC-rich DNA templates).
Vials included in the kit:
FIREPol® DNA Polymerase 1x1000 U | 200 µl 10x Reaction Buffer B 1x5 ml 10x Reaction Buffer BD 1x5 ml 10x Solution S 1x0.5 ml 25 mM MgCl2 1x5 ml
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
dNTPs: dATP, dCTP, dGTP and dTTP
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