Burgener, I et al; Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol 2008, 124; 184
Meide van der, PH et al; Monoclonal antibodies to human immune interferon and their use in a sensitive solid-phase ELISA. J Immunol Methods 1985, 79: 293
Monti, P et al; The CC Chemokine MCP-1/CCL2 in pancreatic cancer progression: regulation expression and potential mechanisms of antimalignant activity. Cancer Res 2003, 63:7451
Rupin, A et al; Inactivation of plasminogen activator inhibitor-1 accelerates thrombolysis of a platelet-rich thrombus in rat mesenteric arterioles. Thromb Haemst 2001, 86:1528
Manitz, M et al; Loss of S100A9 (MRP14) results in reduced interleukin-8-induced CD11b surface expression, a polarized microfilament system, and diminished responsiveness to chemoattractants in vitro. Mol Cell Biol 2003, 23: 1034
The human Bone Morphogenetic protein 2 (BMP-2) Kit is a sandwich ELISA. The capture antibody is a monoclonal BMP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known BMP-2 concentration are added to these wells and allowed to complex with the bound BMP-2 antibody. A biotinylated BMP-2 monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of BMP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human BMP-2 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse, Human, Rat
Immunogen:
rHuman BMP- Q283-R396 expressed in an mammalian expression system and purified.
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
BMP2; bone morphogenetic protein 2; BMP2A; BMP-2A;
Accession Number:
P12643 BMP2_HUMAN;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Human; Mouse; Rat; | The antibodies in this kit cross react with human, rat and mouse BMP-2 and likely other untested species.
The rat Bone Morphogenetic protein 2 (BMP-2) Kit is a sandwich ELISA. The capture antibody is a monoclonal BMP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known BMP-2 concentration are added to these wells and allowed to complex with the bound BMP-2 antibody. A biotinylated BMP-2 monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of BMP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat BMP-2 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse, Human, Rat
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids Q283-R396
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
BMP2; bone morphogenetic protein 2; BMP2A; BMP-2A;
Accession Number:
P49001 BMP2_RAT;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Human; Mouse; Rat; | The antibodies in this kit cross react with human, rat and mouse BMP-2 and likely other untested species.
The mouse Bone Morphogenetic protein 2 (BMP-2) Kit is a sandwich ELISA. The capture antibody is a monoclonal BMP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known BMP-2 concentration are added to these wells and allowed to complex with the bound BMP-2 antibody. A biotinylated BMP-2 monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of BMP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse BMP-2 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse, Human, Rat
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids Q283-R396
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
BMP2; bone morphogenetic protein 2; BMP2A; BMP-2A;
Accession Number:
P21274 BMP2_MOUSE;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Human; Mouse; Rat; | The antibodies in this kit cross react with human, rat and mouse BMP-2 and likely other untested species.
Assay Range:
31.2 pg/mL - 2000 pg/mL
Sensitivity:
< 2 pg/mL
References:
1. Machado R et al (2012) Elastin-based nanoparticles for delivery of bone morphogenetic proteins. Methods Mol Biol. 2012;906:353-63.
The human Bone morphogenetic protein 4 (BMP-4) Kit is a sandwich ELISA. The capture antibody is a monoclonal human BMP-4 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known BMP-4 concentration are added to these wells and allowed to complex with the bound BMP-4 antibody. A biotinylated human BMP-4 monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of BMP-4 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human BMP-4 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids S293-R408
Mouse and rat leptin sequences show >80% amino identity with human leptin but direct cross reactivity with this kit is untested.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
BMP4; bone morphogenetic protein 4; BMP2B; BMP-2B; BMP2B1; BMP-4;
Accession Number:
P12644 BMP4_HUMAN;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human CD40L Kit is a sandwich ELISA. The capture antibody is a polyclonal human CD40L antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known CD40L concentration are added to these wells and allowed to complex with the bound CD40L antibody. A biotinylated human CD40L polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD40L present in the samples. The purpose of this kit is the in-vitro quantitative determination of human CD40L in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
CD40L is the ligand for CD40, a member of the tumour necrosis factor (TNF) receptor superfamily. CD40L is expressed mainly on activated CD4+ T-lymphocytes as a single-pass type II membrane protein. Proteolytic processing of the membrane form results in a soluble secreted form of CD40L.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from an The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids expression system consisting of amino acids, E108-L261
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Epidermal Growth Factor (EGF) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse EGF antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known EGF concentration are added to these wells and allowed to complex with the bound EGF antibody. A biotinylated mouse EGF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of EGF present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse EGF in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, N977-R1029
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Fractalkine Kit is a sandwich ELISA. The capture antibody is a polyclonal human Fractalkine antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Fractalkine concentration are added to these wells and allowed to complex with the bound Fractalkine antibody. A biotinylated human Fractalkine polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Fractalkine present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Fractalkine in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen used for this human Fractalkine ELISA is recombinant human Fractalkine amino acids 25-339 that was produced in and purified from a mammalian cell expression system.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Try our new GDNF Rapid ELISA kit (BEK-2230-1P). Same specificity, more sensitivity and faster! The rat GDNF Kit is a sandwich ELISA. The capture antibody is a monoclonal anti-rat GDNF antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known GDNF concentration are added to these wells and allowed to complex with the bound GDNF antibody. A biotinylated ant-rat GDNF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of GDNF present in the samples. The purpose of this kit is for quantitative detection of rat GDNF in cell culture supernatants, cell lysates, tissue homogenates, serum, and heparin or EDTA treated plasma samples only when used as directed. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Rat
Immunogen:
The standard and immunogen used in this kit is recombinant mature (aa78-211) rat GDNF produced in and purified from an insect cell expression system. This assay will not distinguish between unprocessed proGDNF and mature GDNF as the antibodies will detect both forms when present.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Rat GDNF & proGDNF | Cross reactivity to human and mouse GDNF is likely because of sequence homology
Assay Range:
31.2 pg/mL - 2000 pg/mL
Sensitivity:
< 4 pg/mL
References:
Simões L et al. (2017) "Prevention of memory impairment and neutrophic factors increased by lithium in Wistar rats submitted to pneumococcal meningitis model." Mediators Inflamm. Sep; [Epub ahead of print]Valvassori SS et al. (2017) "The different effects of lithium and tamoxifen on memory formation and the levels of neurotrophic factors in the brain of male and female rats." Brain Res Bull. Sep;134:228-35Varela RB et al. (2015) "Sodium butyrate and mood stabilizers block ouabain-induced hyperlocomotion and increase BDNF, NGF and GDNF levels in brain of Wistar rats." J Psychiatr Res. Feb;61:114-121.Barichello T et al. (2015) "Sodium Butyrate Prevents Memory Impairment by Re-establishing BDNF and GDNF Expression in Experimental Pneumococcal Meningitis." Mol Neurobiol. Aug;52(1):734-40.Leffa DD et al. (2015) "Effects of palatable cafeteria diet on cognitive and noncognitive behaviors and brain neurotrophins' levels in mice." Metab Brain Dis. Aug;30(4):1073-82.
The mouse ICAM-1 Kit is a sandwich ELISA. The capture antibody is a mouse ICAM-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known ICAM-1 concentration are added to these wells and allowed to complex with the bound ICAM-1 antibody. A biotinylated mouse ICAM-1 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of ICAM-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse ICAM-1 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
Intercellular adhesion molecule 1 (ICAM1) is a single-pass type 1 membrane glycoprotein that belongs to the ICAM family. ICAM1 is typically expressed on endothelial cells and cells of the immune system.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids Q28-N485
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Mouse ICAM-1
Assay Range:
156 pg/mL - 10000 pg/mL
Sensitivity:
< 10 pg/mL
References:
Raoufi-Rad, N. et al. (2017) In vivo imaging of endothelial cell adhesion molecule expression after radiosurgery in an animal model of arteriovenous malformation. PLoS One. 12(9):e0185393 Application: Cultured cells. Species: Mouse
The rat ICAM-1 Kit is a sandwich ELISA. The capture antibody is a mouse monoclonal ICAM-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known ICAM-1 concentration are added to these wells and allowed to complex with the bound ICAM-1 antibody. A biotinylated rat ICAM-1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of ICAM-1 present in the samples. The purpose of this kit is the in-vitro quantitative detection of rat ICAM-1/CD54 in cell culture supernatants, cell lysates, serum, and heparin or EDTA treated plasma samples only when used as directed. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
Intercellular adhesion molecule 1 (ICAM1) is a single-pass type 1 membrane glycoprotein that belongs to the ICAM family. ICAM1 is typically expressed on endothelial cells and cells of the immune system.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Rat
Immunogen:
The standard product used in this kit is recombinant rat ICAM-1/CD54, consisting amino acids 28-493 expressed in and purified from a mammalian cell expression system.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Interferon gamma Kit is a sandwich ELISA. The capture antibody is a monoclonal Interferon gamma antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known Interferon gamma concentration are added to these wells and allowed to complex with the bound Interferon gamma antibody. A biotinylated mouse Interferon gamma polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Interferon gamma present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Interferon gamma in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, H23-C155
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Ifng;
Accession Number:
P01580 IFNG_MOUSE;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Insulin-like growth factor-binding protein 1 (IGFBP-1) Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse IGFBP-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known IGFBP-1 concentration are added to these wells and allowed to complex with the bound IGFBP-1 antibody. A biotinylated mouse IGFBP-1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IGFBP-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IGFBP-1 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A26-N272
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
IBP-1; IGF-binding protein 1; IGFBP-1; Igfbp1; Igfbp-1;
Accession Number:
P47876 IBP1_MOUSE;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Mouse Insulin-like growth factor-binding protein 1
The mouse Interleukin 3 (IL-3) Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse IL-3 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known IL-3 concentration are added to these wells and allowed to complex with the bound IL-3 antibody. A biotinylated mouse IL-3 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-3 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-3 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, A27-C166
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Interleukin 13 (IL-13) Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse IL-13 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known IL-13 concentration are added to these wells and allowed to complex with the bound IL-13 antibody. A biotinylated mouse IL-13 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-13 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-13 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, S26-F131
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Interleukin-13; IL-13; IL13; NC30;
Accession Number:
P20109 IL13_MOUSE; P35225 IL13_HUMAN;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Interleukin 17 (IL-17) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse IL-17 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known IL-17 concentration are added to these wells and allowed to complex with the bound IL-17 antibody. A biotinylated mouse IL-17 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-17 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-17A in cell culture supernatants, cell lysates, serum, and EDTA or heparin plasma samples only when used as directed. For research use only, not for clinical or diagnostic purposes.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard product used in this kit is recombinant mouse IL-17A, consisting amino acids 22-158 expressed in and purified from an E. coli expression system.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Macrophage colony-stimulating factor 1 (M-CSF) Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse M-CSF antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known M-CSF concentration are added to these wells and allowed to complex with the bound M-CSF antibody. A biotinylated mouse M-CSF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of M-CSF present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse M-CSF in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, K33-E262
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
MCSF; CSF-1;
Accession Number:
P07141 CSF1_MOUSE;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse C-C motif chemokine 22 (CCL22) or Macrophage-derived chemokine (MDC) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse MDC antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known MDC concentration are added to these wells and allowed to complex with the bound MDC antibody. A biotinylated mouse MDC polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MDC present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse MDC in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, G25-S92
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Activated B and dendritic cell-derived; CC chemokine ABCD-1; Small-inducible cytokine A22; Ccl22; Abcd1; Scya22; MDC;
Accession Number:
O88430 CCL22_MOUSE;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human MIP-3 alpha (MIP-3a) Kit is a sandwich ELISA. The capture antibody is a monoclonal human MIP-3a antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MIP-3a concentration are added to these wells and allowed to complex with the bound MIP-3a antibody. A biotinylated human MIP-3a polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MIP-3a present in the samples. The purpose of this kit is the in-vitro quantitative determination of human MIP-3a in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, A27-M96
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
C-C motif chemokine 20; Small-inducible cytokine A20; Macrophage inflammatory protein 3 alpha; Liver and activation-regulated chemokine; CCL20; LARC; MIP3A; SCYA20;
Accession Number:
P78556 CCL20_HUMAN;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Matrix metalloproteinase-1 (MMP-1) Kit is a sandwich ELISA. The capture antibody is a polyclonal human MMP-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MMP-1 concentration are added to these wells and allowed to complex with the bound MMP-1 antibody. A biotinylated human MMP-1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MMP-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human MMP-1 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids F20-N469
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Matrix metalloproteinase-2 (MMP-2) Kit is a sandwich ELISA. The capture antibody is a polyclonal human MMP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MMP-2 concentration are added to these wells and allowed to complex with the bound MMP-2 antibody. A biotinylated human MMP-2 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MMP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human MMP-2 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A30-C660
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
72 kDa type IV collagenase; EC 3.4.24.24; 72 kDa gelatinase; Matrix metalloproteinase-2; MMP-2; Gelatinase A; TBE-1; MMP2; CLG4A;
Accession Number:
P08253 MMP2_HUMAN;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Human MMP-2
Assay Range:
625 pg/mL - 40000 pg/mL
Sensitivity:
< 10 pg/mL
References:
Abramov D (2017) Compositions and Methods for Treating Post-Operative Complications of Cardiopulmonary Surgery. United States Patent Application 20170354722 Application: Human blood.
The human Matrix metalloproteinase-3 (MMP-3) Kit is a sandwich ELISA. The capture antibody is a polyclonal human MMP-3 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MMP-3 concentration are added to these wells and allowed to complex with the bound MMP-3 antibody. A biotinylated human MMP-3 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MMP-3 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human MMP-3 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids Y18-C477
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human MMP-7 Kit is a sandwich ELISA. The capture antibody is a polyclonal human MMP-7 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MMP-7 concentration are added to these wells and allowed to complex with the bound MMP-7 antibody. A biotinylated human MMP-7 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MMP-7 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human MMP-7 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids L18-K267
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Accession Number:
https://www.uniprot.org/uniprot/P09237
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Matrix metalloproteinase-8 (MMP-8) Kit is a sandwich ELISA. The capture antibody is a polyclonal human MMP-8 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MMP-8 concentration are added to these wells and allowed to complex with the bound MMP-8 antibody. A biotinylated human MMP-8 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MMP-8 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human MMP-8 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Matrix metalloproteinase-9 (MMP-9) Kit is a sandwich ELISA. The capture antibody is a monoclonal human MMP-9 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MMP-9 concentration are added to these wells and allowed to complex with the bound MMP-9 antibody. A biotinylated human MMP-9 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MMP-9 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human MMP-9 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human, Mouse (Negative), Rat (Negative)
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A20-D707
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
MMP-9; MMP9; Matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB; CLG4B;
Accession Number:
P14780 MMP9_HUMAN;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Detects human pro and active MMP-9 and MMP-9 bound to TIMP. | Human; does not react with rat or mouse MMP-9
The mouse Matrix metalloproteinase-9 (MMP-9) Kit is a sandwich ELISA. The capture antibody is a mouse MMP-9 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known MMP-9 concentration are added to these wells and allowed to complex with the bound MMP-9 antibody. A biotinylated mouse MMP-9 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MMP-9 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse MMP-9 in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A20-P730
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
MMP-9; MMP9; Matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB; CLG4B;
Accession Number:
P41245 MMP9_MOUSE;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Mouse MMP-9
Assay Range:
156 pg/mL - 10000 pg/mL
Sensitivity:
< 20 pg/mL
References:
Rohl J (2017) Intracellular trafficking and secretion of matrix metalloproteinases during macrophage migration. PhD Thesis Queensland University of Technology, Australia. Wang Y et al. (2014) Influence of apolipoprotein E, age and aortic site on calcium phosphate induced abdominal aortic aneurysm in mice. Atherosclerosis. 2014 Jul;235(1):204-12. Moxon JV et al. (2014) Proteomic and genomic analyses suggest the association of apolipoprotein C1 with abdominal aortic aneurysm. Proteomics Clin Appl. 2014 Oct;8(9-10):762-72.
The mouse Osteoprotegerin (OPG) Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse OPG antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known OPG concentration are added to these wells and allowed to complex with the bound OPG antibody. A biotinylated mouse OPG polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of OPG present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse OPG in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids E22-L401
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Osteoclastogenesis inhibitory factor; Tumor necrosis factor receptor superfamily member 11B;
Accession Number:
O08712 TR11B_MOUSE;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Osteopontin (OPN) Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse OPN antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known OPN concentration are added to these wells and allowed to complex with the bound OPN antibody. A biotinylated mouse OPN polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of OPN present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse OPN in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids L17-N294
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Placenta growth factor (PLGF) Kit is a sandwich ELISA. The capture antibody is a polyclonal human PLGF antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known PLGF concentration are added to these wells and allowed to complex with the bound PLGF antibody. A biotinylated human PLGF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of PLGF present in the samples. The purpose of this kit is the in-vitro quantitative determination of human PLGF in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, A21-R149
There is no detectable cross-reactivity with Eotaxin, MCP-1, MCP-2, 6Ckine, MARC.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
PGFL; PLGF;
Accession Number:
P49763 PLGF_HUMAN;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Tyrosine-protein kinase receptor TIE-2 Kit is a sandwich ELISA. The capture antibody is a polyclonal human TIE2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TIE2 concentration are added to these wells and allowed to complex with the bound TIE2 antibody. A biotinylated human TIE2 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TIE2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TIE2 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A23-K745
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Metalloproteinase inhibitor 2 (TIMP-2) Kit is a sandwich ELISA. The capture antibody is a monoclonal human TIMP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TIMP-2 concentration are added to these wells and allowed to complex with the bound TIMP-2 antibody. A biotinylated human TIMP-2 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TIMP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TIMP-2 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The matrix metalloproteinases (MMPs) are a large family of¶Ã¿ zinc endopeptidases that¶Ã¿ degrade extracellular matrix components such as fibronectin and collagen type III. The tissue inhibitors of metalloproteinase (TIMPs) complex with these matrix metalloproteinases and irreversibly inactivate them. The TIMP proteins are¶Ã¿ homologous and are either secreted in soluble form (TIMP1, TIMP2 and TIMP4) or bind to extracellular matrix components (TIMP3). Metalloproteinase inhibitor 2 (TIMP2) is known to inhibit MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Tissue inhibitor of metalloproteinases 2; TIMP-2; CSC-21K; TIMP2;
Accession Number:
P16035 TIMP2_HUMAN;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Metalloproteinase inhibitor 3 (TIMP-3) Kit is a sandwich ELISA. The capture antibody is a monoclonal human TIMP-3 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TIMP-3 concentration are added to these wells and allowed to complex with the bound TIMP-3 antibody. A biotinylated human TIMP-3 monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TIMP-3 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TIMP-3 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and antigen is recombinant TIMP-3 amino acids 24-211 produced in and purified from mammalian cell line tissue culture.
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Tissue inhibitor of metalloproteinases 3; TIMP-3; MIG-5; TIMP3;
Accession Number:
P35625 TIMP3_HUMAN;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human E-Cadherin Kit is a sandwich ELISA. The capture antibody is a polyclonal human E-Cadherin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known E-Cadherin concentration are added to these wells and allowed to complex with the bound E-Cadherin antibody. A biotinylated human E-Cadherin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of E-Cadherin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human E-Cadherin in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids D155-I707
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Cardiotrophin-1 (CT-1) Kit is a sandwich ELISA. The capture antibody is a polyclonal human CT-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known CT-1 concentration are added to these wells and allowed to complex with the bound CT-1 antibody. A biotinylated human CT-1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CT-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human CT-1 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, S2-A201
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
CTF1
Accession Number:
Q16619 CTF1_HUMAN;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Growth Hormone Kit is a sandwich ELISA. The capture antibody is a polyclonal human Growth Hormone antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Growth Hormone concentration are added to these wells and allowed to complex with the bound Growth Hormone antibody. A biotinylated human Growth Hormone polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Growth Hormone present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Growth Hormone in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids F27-F217
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Resistin Kit is a sandwich ELISA. The capture antibody is a polyclonal human Resistin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Resistin concentration are added to these wells and allowed to complex with the bound Resistin antibody. A biotinylated human Resistin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Resistin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Resistin in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids P2-A115
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The rat Metalloproteinase inhibitor 1 (TIMP-1) Kit is a sandwich ELISA. The capture antibody is a polyclonal rat TIMP-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known TIMP-1 concentration are added to these wells and allowed to complex with the bound TIMP-1 antibody. A biotinylated rat TIMP-1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TIMP-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat TIMP-1 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Rat
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids C24-A217
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Tissue inhibitor of metalloproteinases 1; Timp1;
Accession Number:
P30120 TIMP1_RAT;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The rat Interleukin 18 (IL-18) Kit is a sandwich ELISA. The capture antibody is a monoclonal rat IL-18 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known IL-18 concentration are added to these wells and allowed to complex with the bound IL-18 antibody. A biotinylated rat IL-18 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-18 present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat IL-18 in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Rat
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, H37-S194
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Interleukin 18 (IL-18) Kit is a sandwich ELISA. The capture antibody is a monoclonal human IL-18 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IL-18 concentration are added to these wells and allowed to complex with the bound IL-18 antibody. A biotinylated anti-human IL-18 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-18 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IL-18 in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, Y33-D197
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Human IL-18
Assay Range:
15.6 pg/mL - 1000 pg/mL
Sensitivity:
< 1 pg/mL
References:
Fernandez MV et al. (2016) Ion efflux and influenza infection trigger NLRP3 inflammasome signaling in human dendritic cells. J Leukoc Biol. 99(5):723-34 Cordero MD et al. (2016) Mutation in cytochrome b gene of mitochondrial DNA in a family with fibromyalgia is associated with NLRP3-inflammasome activation. J Met Genet. 53(2):113-22 Bullón P et al. (2016) AMPK Phosphorylation Modulates Pain by Activation of NLRP3 Inflammasome. Antioxid Redox Signal. 24(2):157-170 Miklaszewska M et al (2015) "Clinical validity of urinary interleukin 18 and interleukin 6 determinations in preterm newborns." Link Przegląd Lekarski 72:589-596; Appl: ELISA; Human UrineCordero MD et al. (2013) NLRP3 Inflammasome is activated in Fibromyalgia: the effect of Coenzyme Q10 PubMed link Antioxid Redox Signal. July 25 2013 Epub ahead of printSaroj Kumar Das Majumdar (2013) Evaluation of the diagnostic performance of new markers for acute kidney injury. Transworld Med. J. 1(1) 1-7 Vijayasimha M et al. (2013) Evaluation of the diagnostic performance of new markers for acute kidney injury associated with contrast administration. J Mahatma Gandhi Inst Med Sci. 18:116-21
The human Adiponectin Kit is a sandwich ELISA. The capture antibody is a monoclonal anti-human Adiponectin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Adiponectin concentration are added to these wells and allowed to complex with the bound Adiponectin antibody. A biotinylated anti-human Adiponectin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Adiponectin present in the samples. The purpose of this kit is for the quantitative detection of Human Adiponectin in cell culture supernatants, serum, heparin or EDTA treated plasma, and urine when used as directed. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
Assay immunogen and standard is recombinant human adiponectin amino acids 19-244 expressed in and purified from a mammalian cell expression system
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Growth-regulated alpha protein (CXCL1) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse CXCL1 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known CXCL1 concentration are added to these wells and allowed to complex with the bound CXCL1 antibody. A biotinylated mouse CXCL1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CXCL1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse CXCL1 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, R20-K96
There is cross-reactivates with TGF beta 2, TGF beta 3, TGF beta 5<1%.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
C-X-C motif chemokine 1; Platelet-derived growth factor-inducible protein KC; Secretory protein N51; Cxcl1; Gro; Gro1; Mgsa; Scyb1;
Accession Number:
P12850 GROA_MOUSE;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The rat Growth-regulated alpha protein (CXCL1) Kit is a sandwich ELISA. The capture antibody is a polyclonal rat CXCL1 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known CXCL1 concentration are added to these wells and allowed to complex with the bound CXCL1 antibody. A biotinylated rat CXCL1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CXCL1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat CXCL1 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Rat
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, A25-K96
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse CXCL10 Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse CXCL10 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known CXCL10 concentration are added to these wells and allowed to complex with the bound CXCL10 antibody. A biotinylated mouse CXCL10 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CXCL10 present in the samples. The purpose of this kit is for quantitative detection of mouse CXCL10/IP-10 in cell culture supernatants, serum, and heparin, plasma samples only when used as directed. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard product used in this kit is recombinant mouse CXCL10/IP-10, consisting amino acids 22-98 expressed in and purified from an E. coli expression system.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human MHC Class I polypeptide-related sequence A (MIC-A) Kit is a sandwich ELISA. The capture antibody is a polyclonal human MICA antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MIC-A concentration are added to these wells and allowed to complex with the bound MICA antibody. A biotinylated human MICA polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MIC-A present in the samples. The purpose of this kit is the in-vitro quantitative detection of human MIC-A in cell culture supernatants, cell lysates, and serum, only when used as directed. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
Antigen and Standard is recombinant human MIC-A amino acids 24-307 produced in and purified from a mammalian cell expression system
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
MIC-A; MICA;
Accession Number:
Q29983 MICA_HUMAN;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human MIF Kit is a sandwich ELISA. The capture antibody is a polyclonal human MIF antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MIF concentration are added to these wells and allowed to complex with the bound MIF antibody. A biotinylated human MIF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MIF present in the samples. The purpose of this kit is the in-vitro quantitative determination of human MIF in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids P2-A115
Bosters human MIF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MIF has been precoated onto 96-well plates. Standards(E.coli, P2-A115) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MIF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MIF amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Lipocalin-2/NGAL Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse Lipocalin-2/NGAL antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known Lipocalin-2/NGAL concentration are added to these wells and allowed to complex with the bound Lipocalin-2/NGAL antibody. A biotinylated mouse Lipocalin-2/NGAL polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Lipocalin-2/NGAL present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Lipocalin-2/NGAL in samples such as sera, plasma, urine and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and antigen for this Lipocalin-2/NGAL ELISA is recombinant mouse Lipocalin-2/NGAL amino acids 21-N200 produced in an purified from a mammalian cell expression system.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The rat Lipocalin-2/NGAL Kit is a sandwich ELISA. The capture antibody is a polyclonal rat Lipocalin-2/NGAL antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known Lipocalin-2/NGAL concentration are added to these wells and allowed to complex with the bound Lipocalin-2/NGAL antibody. A biotinylated rat Lipocalin-2/NGAL polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Lipocalin-2/NGAL present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat Lipocalin-2/NGAL in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Rat
Immunogen:
The standard and antigen for this Lipocalin-2/NGAL ELISA is recombinant rat Lipocalin-2/NGAL amino acids 21-N198 produced in an purified from a mammalian cell expression system.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Adiponectin Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse Adiponectin antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known Adiponectin concentration are added to these wells and allowed to complex with the bound Adiponectin antibody. A biotinylated mouse Adiponectin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Adiponectin present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Adiponectin in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids E18-N244
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Mouse Adiponectin
Assay Range:
312 pg/mL - 20000 pg/mL
Sensitivity:
< 10 pg/mL
References:
Park JH et al. (2014) Anti-Adipogenic Activity of Ailanthoidol on 3T3-L1 Adipocytes. Biomedical Science Letters 2014;20:62-69
The human Lipocalin-2 Kit is a sandwich ELISA. The capture antibody is a polyclonal human Lipocalin-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Lipocalin-2 concentration are added to these wells and allowed to complex with the bound Lipocalin-2 antibody. A biotinylated human Lipocalin-2 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Lipocalin-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Lipocalin-2 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and antigen for this Lipocalin-2/NGAL ELISA is recombinant human Lipocalin-2/NGAL amino acids 21-N198 produced in an purified from a mammalian cell expression system.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Plasminogen activator inhibitor 1 (PAI-1) Kit is a sandwich ELISA. The capture antibody is a polyclonal human PAI-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known PAI-1 concentration are added to these wells and allowed to complex with the bound PAI-1 antibody. A biotinylated human PAI-1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of PAI-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human PAI-1 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from an insect expression system consisting of amino acids S22-P402
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human CXCL9 Kit is a sandwich ELISA. The capture antibody is a polyclonal human CXCL9 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known CXCL9 concentration are added to these wells and allowed to complex with the bound CXCL9 antibody. A biotinylated human CXCL9 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CXCL9 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human CXCL9 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, T23-T125
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse CXCL16 Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse CXCL16 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known CXCL16 concentration are added to these wells and allowed to complex with the bound CXCL16 antibody. A biotinylated mouse CXCL16 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CXCL16 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse CXCL16 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, N27-P114
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
C-X-C motif chemokine 16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SR-PSOX; Small-inducible cytokine B16; Transmembrane chemokine CXCL16; Srpsox;
Accession Number:
Q8BSU2 CXL16_MOUSE;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Cystatin C Kit is a sandwich ELISA. The capture antibody is a polyclonal human Cystatin C antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Cystatin C concentration are added to these wells and allowed to complex with the bound Cystatin C antibody. A biotinylated human Cystatin C polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Cystatin C present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Cystatin C in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids M1-A146
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Fractalkine/CX3CL1 Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse Fractalkine/CX3CL1 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known Fractalkine/CX3CL1 concentration are added to these wells and allowed to complex with the boundFractalkine/CX3CL1 antibody. A biotinylated anti-mouse Fractalkine/CX3CL1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Fractalkine/CX3CL1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Fractalkine/CX3CL1 in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen used in this mouse Fractalkine/CX3CL1 ELISA kit is recombinant mouse Fractalkine/CX3CL1 that is produced in and purified from an sf21 insect cell expression system. The recombinant protein material is highly purified and contains no infectious agents.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Interleukin 1 beta (IL-1b) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse IL-1b antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known IL-1b concentration are added to these wells and allowed to complex with the bound IL-1b antibody. A biotinylated mouse IL-1b polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1b present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-1b in samples such as sera, plasma, cell lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, V118-S269
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Interleukin-1b; IL-1b; IL1b;
Accession Number:
https://www.uniprot.org/uniprot/P10749
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Mouse IL-1b
Assay Range:
12.5pg/mL - 800pg/mL
Sensitivity:
< 1 pg/mL
References:
Kunos, G, Jourdan T, Michael P, Aouadi M (2016), Glucan-Encapsulated siRNA for Treating Type 2 Diabetes Mellitus. US Patent Application 20160138028 Jourdan T, Godlewski G, Cinar R, Bertola A, Szanda G, Liu J, Tam J, Han T, Mukhopadhyay B, Skarulis MC, Ju C, Aouadi M, Czech MP, Kunos G. (2013) Activation of the Nlrp3 inflammasome in infiltrating macrophages by endocannabinoids mediates beta cell loss in type 2 diabetes. Nat. Med Sep;19(9):1132-40 2013 [E print]
The human Endoglin/CD105 Kit is a sandwich ELISA. The capture antibody is a monoclonal human Endoglin/CD105 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Endoglin/CD105 concentration are added to these wells and allowed to complex with the bound Endoglin/CD105 antibody. A biotinylated human Endoglin/CD105 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Endoglin/CD105 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Endoglin/CD105 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids E26-G586
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
CD105; ENG; END;
Accession Number:
P17813 EGLN_HUMAN;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human MPO Kit is a sandwich ELISA. The capture antibody is a polyclonal human MPO antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known MPO concentration are added to these wells and allowed to complex with the bound MPO antibody. A biotinylated human MPO polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MPO present in the samples. The purpose of this kit is the in-vitro quantitative determination of human MPO in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A49-S745
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
MPO; EC 1.11.1.7; Myeloperoxidase;
Accession Number:
P05164 PERM_HUMAN;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human P-Cadherin Kit is a sandwich ELISA. The capture antibody is a polyclonal human P-Cadherin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known P-Cadherin concentration are added to these wells and allowed to complex with the bound P-Cadherin antibody. A biotinylated human P-Cadherin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of P-Cadherin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human P-Cadherin in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
Cadherins are transmembrane glycoproteins that mediate calcium ion-dependent cell adhesion. The cadherin family includes N-, P-, R-, B- and E-cadherins which are found in adherens junctions.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Cathepsin B Kit is a sandwich ELISA. The capture antibody is a polyclonal human Cathepsin B antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Cathepsin B concentration are added to these wells and allowed to complex with the bound Cathepsin B antibody. A biotinylated human Cathepsin B antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Cathepsin B present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Cathepsin B in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids R18-I339
Bosters human Cathepsin B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cathepsin B has been precoated onto 96-well plates. Standards(NSO, R18-I339) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cathepsin B amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
APP secretase; APPS; Cathepsin B1; CTSB; CPSB;
Accession Number:
P07858 CATB_HUMAN;
Kit Components:
The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Human Cathepsin B
Assay Range:
156 pg/mL - 10000 pg/mL
Sensitivity:
< 5 pg/mL
References:
1. Gogiel T et al (2012) Differential distribution of cathepsin B in human umbilical cord tissues Acta Biochim Pol. 2012;59(4):679-84
The mouse CD30 ligand (CD30L) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse CD30L antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known CD30L concentration are added to these wells and allowed to complex with the bound CD30L antibody. A biotinylated mouse CD30L polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD30L present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse CD30L in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids Q68-D239
Bosters mouse CD30L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD30L has been precoated onto 96-well plates. Standards(NSO, Q68-D239) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD30L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD30L amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Tumor necrosis factor ligand superfamily member 8; CD153; Tnfsf8;
Accession Number:
P32972 TNFL8_MOUSE;
Kit Components:
The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Granulocyte Colony-Stimulating factor (G-CSF) Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse G-CSF antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known G-CSF concentration are added to these wells and allowed to complex with the bound G-CSF antibody. A biotinylated mouse G-CSF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of G-CSF present in the samples. The purpose of this kit is the in-vitro quantitative determination of G-CSF in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, V31-A208
Bosters mouse G-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for G-CSF has been precoated onto 96-well plates. Standards(E.coli, V31-A208) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for G-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse G-CSF amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Pluripoietin; G-CSF; Csf3; Csfg;
Accession Number:
P09920 CSF3_MOUSE;
Kit Components:
The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Try our new GDNF Rapid ELISA kit (BEK-2222-1P). Same specificity, more sensitivity and faster!The human GDNF Kit is a sandwich ELISA. The capture antibody is a monoclonal human GDNF antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known GDNF concentration are added to these wells and allowed to complex with the bound GDNF antibody. A biotinylated human GDNF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of GDNF present in the samples. The purpose of this kit is the in-vitro quantitative determination of GDNF in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen used for this ELISA is Recombinant protein standard manufactured in a mouse myeloma (NSO) expression system, amino acids 109-211 for human GDNF.
Bosters human GDNF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GDNF has been precoated onto 96-well plates. Standards(NSO, R109-I211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GDNF amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Human Glial cell line-derived neurotrophic factor/GDNF/ATF
The human TRAIL Kit is a sandwich ELISA. The capture antibody is a monoclonal anti-human TRAIL antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TRAIL concentration are added to these wells and allowed to complex with the bound TRAIL antibody. A biotinylated human TRAIL polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TRAIL present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TRAIL in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids T95-G281
Bosters human TRAIL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TRAIL has been precoated onto 96-well plates. Standards (NSO,T95-G281) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TRAIL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TRAIL amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Resistin Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse Resistin antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known Resistin concentration are added to these wells and allowed to complex with the bound Resistin antibody. A biotinylated mouse Resistin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Resistin present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Resistin in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, S21-S114
Bosters mouse Resistin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Resistin has been precoated onto 96-well plates. Standards(E.coli, S21-S114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Resistin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Resistin amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Resistin; Adipose tissue-specific secretory factor; ADSF; Adipose-specific cysteine-rich secreted protein A12-alpha; Cysteine-rich secreted protein FIZZ3; Retn; Fizz3;
Accession Number:
Q99P87 RETN_MOUSE;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Cathepsin B Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse Cathepsin B antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known Cathepsin concentration are added to these wells and allowed to complex with the bound Cathepsin antibody. A biotinylated mouse Cathepsin B antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Cathepsin present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Cathepsin in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. The cell line used to make Cathepsin B standard in the mouse Cathepsin kit is NSO cells; they are murine B-cell plasmacytoma line.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids H18-F339
Bosters mouse Cathepsin B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cathepsin B has been precoated onto 96-well plates. Standards(NSO, H18-F339) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Cathepsin B amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Cathepsin B1; Ctsb;
Accession Number:
P10605 CATB_MOUSE;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse CD80 Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse CD80 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known CD80 concentration are added to these wells and allowed to complex with the bound CD80 antibody. A biotinylated mouse CD80 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD80 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse CD80 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids D37-K245
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Emmprin Kit is a sandwich ELISA. The capture antibody is a polyclonal human Emmprin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Emmprin concentration are added to these wells and allowed to complex with the bound Emmprin antibody. A biotinylated human Emmprin monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Emmprin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Emmprin in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids T25-H205
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Human Emmprin/CD147
Assay Range:
31.2 pg/mL - 2000 pg/mL
Sensitivity:
< 2 pg/mL
References:
1. Lu Q et al (2013) Expression and clinical significance of extracellular matrix metalloproteinase inducer, EMMPRIN/CD147, in human osteosarcoma. Oncol Lett. 2013 Jan;5(1):201-207
The mouse Galectin-1 Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse Galectin-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known Galectin-1 concentration are added to these wells and allowed to complex with the bound Galectin-1 antibody. A biotinylated mouse Galectin-1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Galectin-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Galectin-1 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, A2-E135
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human RBP4 Kit is a sandwich ELISA. The capture antibody is a polyclonal human RBP4 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known RBP4 concentration are added to these wells and allowed to complex with the bound RBP4 antibody. A biotinylated human RBP4 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of RBP4 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human RBP4 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids E19-L201
Bosters human RBP4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for RBP4 has been precoated onto 96-well plates. Standards(NSO, E19-L201) and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for RBP4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human RBP4 amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Retinol-binding protein 4; Plasma retinol-binding protein; PRBP; RBP; RBP4;
Accession Number:
P02753 RET4_HUMAN;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse TrkB Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse TrkB antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known TrkB concentration are added to these wells and allowed to complex with the bound TrkB antibody. A biotinylated mouse TrkB polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TrkB present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse TrkB in samples such as tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids C32-H429
Bosters mouse TrkB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TrkB has been precoated onto 96-well plates. Standards (NSO,C32-H429) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TrkB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TrkB amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Mouse TrkB
Assay Range:
93.8 pg/mL - 6000 pg/mL
Sensitivity:
< 5 pg/mL
References:
Maejima H, Kanemura N, Kokubun T, Murata K, Takayanagi K (2017) Exercise enhances cognitive function and neurotrophin expression in the hippocampus accompanied by changes in epigenetic programming in senescence-accelerated mice. Neurosci Lett. doi: 10.1016/j.neulet.2017.11.023. [Epub ahead of print]. Application: Mouse hippocampus homogenates.
The mouse MIP-2 Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse MIP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known MIP-2 concentration are added to these wells and allowed to complex with the bound MIP-2 antibody. A biotinylated anti-mouse MIP-2 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MIP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse MIP-2 in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, A28-N100
Bosters mouse MIP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MIP-2 has been precoated onto 96-well plates. Standards(E.coli, A28-N100) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MIP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MIP-2 amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
MP26; Major intrinsic protein; MIP;
Accession Number:
https://www.uniprot.org/uniprot/P10889
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Mouse MIP-2
Assay Range:
15.6 pg/mL - 1000 pg/mL
Sensitivity:
< 5 pg/mL
References:
Kanaya K et al. (2014) Innate immune responses and neuroepithelial degeneration and regeneration in the mouse olfactory mucosa induced by intranasal administration of Poly(I:C). Cell Tissue Res. 2014 Jul;357(1):279-99.
The human Matrix metalloproteinase-13 (MMP-13) Kit is a sandwich ELISA. The capture antibody is a polyclonal human MMP-13 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known MMP-13 concentration are added to these wells and allowed to complex with the bound MMP-13 antibody. A biotinylated human polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MMP-13 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human MMP-13 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids L20-C471
Bosters human MMP13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP13 has been precoated onto 96-well plates. Standards(NSO, L20-C471) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP13 amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Tumor Necrosis Factor receptor 1 (TNF-R1) Kit is a sandwich ELISA. The capture antibody is a monoclonal human TNF-R1 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known TNF-R1 concentration are added to these wells and allowed to complex with the bound TNF-R1 antibody. A biotinylated human polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TNF-R1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TNF-R1 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids L30-T211
Bosters human TNFsR?ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFsR? has been precoated onto 96-well plates. Standards (NSO,L30-T211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFsR? is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFsR? amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse E-cadherin Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse E-cadherin antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known E-cadherin concentration are added to these wells and allowed to complex with the bound E-cadherin antibody. A biotinylated polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of E-cadherin present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse E-cadherin in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids D157-V709
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse C-C motif chemokine 2 (Ccl2) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse Ccl2 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known Ccl2 concentration are added to these wells and allowed to complex with the bound Ccl2 antibody. A biotinylated polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Ccl2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Ccl2 in samples such as sera and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, Q24-R96
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
C-C motif chemokine 2; Monocyte chemoattractant protein 1; Monocyte chemotactic protein 1; MCP-1; Platelet-derived growth factor-inducible protein JE;Small-inducible cytokine A2; Ccl2; Je; Mcp1; Scya2;
Accession Number:
P10148 CCL2_MOUSE;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human B-cell-activating factor (BAFF) Kit is a sandwich ELISA. The capture antibody is a polyclonal human BAFF antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known BAFF concentration are added to these wells and allowed to complex with the bound BAFF antibody. A biotinylated human monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of BAFF present in the samples. The purpose of this kit is the in-vitro quantitative determination of human BAFF in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A134-L285
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse B-cell-activating factor (BAFF) Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse BAFF antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known BAFF concentration are added to these wells and allowed to complex with the bound BAFF antibody. A biotinylated mouse polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of BAFF present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse BAFF in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A127-L309
Bosters mouse BAFF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BAFF has been precoated onto 96-well plates. Standards(NSO, A127-L309) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BAFF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BAFF amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Tumor necrosis factor ligand superfamily member 13B; B lymphocyte stimulator; BLyS; B-cell-activating factor; BAFF; Dendritic cell-derived TNF-like molecule; TNF- and APOL-related leukocyte expressed ligand 1; TALL-1; CD257; TNFSF13B;
Accession Number:
Q9WU72 TN13B_MOUSE;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Cystatin C Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse Cystatin C antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known Cystatin C concentration are added to these wells and allowed to complex with the bound Cystatin C antibody. A biotinylated mouse polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Cystatin C present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Cystatin C in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids M1-A140
Bosters mouse Cystatin C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cystatin C has been precoated onto 96-well plates. Standards(NSO, M1-A140) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cystatin C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Cystatin C amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Cystatin-C; Cystatin-3; Cst3;
Accession Number:
P21460 CYTC_MOUSE;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human C-C motif chemokine 17 (CCL17) Kit is a sandwich ELISA. The capture antibody is a polyclonal human CCL17 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known CCL17 concentration are added to these wells and allowed to complex with the bound CCL17 antibody. A biotinylated human polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CCL17 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human CCL17 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, A24-S94
Bosters human CCL17 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL17 has been precoated onto 96-well plates. Standards(E.coli, A24-S94) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL17 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL17 amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
CC chemokine TARC; Small-inducible cytokine A17; Thymus and activation-regulated chemokine; CCL17; SCYA17; TARC;
Accession Number:
Q92583 CCL17_HUMAN;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human CD40 Kit is a sandwich ELISA. The capture antibody is a polyclonal human CD40 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known CD40 concentration are added to these wells and allowed to complex with the bound CD40 antibody. A biotinylated human polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD40 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human CD40 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids E21-R193
Bosters human CD40 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD40 has been precoated onto 96-well plates. Standards(NSO, E21-R193) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD40 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD40 amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse CD40 Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse CD40 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known CD40 concentration are added to these wells and allowed to complex with the bound CD40 antibody. A biotinylated mouse polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD40 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse CD40 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids V24-R193
Bosters mouse CD40 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD40 has been precoated onto 96-well plates. Standards(NSO, V24-R193) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD40 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD40 amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human receptor tyrosine-protein kinase erbB-2 Kit is a sandwich ELISA. The capture antibody is a polyclonal human erbB-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known erbB-2 concentration are added to these wells and allowed to complex with the bound erbB-2 antibody. A biotinylated human polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of erbB-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human erbB-2 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids T23-T652
Bosters human ErbB-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ErbB-2 has been precoated onto 96-well plates. Standards(NSO, T23-T652) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ErbB-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ErbB-2 amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Alpha-2-HS-glycoprotein (AHSG) Kit is a sandwich ELISA. The capture antibody is a polyclonal human AHSG antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known AHSG concentration are added to these wells and allowed to complex with the bound AHSG antibody. A biotinylated human polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of AHSG present in the samples. The purpose of this kit is the in-vitro quantitative determination of human AHSG in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A19-V367
Bosters human Fetuin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Fetuin A has been precoated onto 96-well plates. Standards(NSO, A19-V367) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Fetuin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fetuin A amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Interleukin 7 (IL-7) Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse IL-7 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known IL-7 concentration are added to these wells and allowed to complex with the bound IL-7 antibody. A biotinylated mouse polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-7 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-7 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, E26-I154
Bosters mouse IL-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-7 has been precoated onto 96-well plates. Standards(E.coli, E26-I154) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-7 amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
IL-7; IL7;
Accession Number:
P10168 IL7_MOUSE;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Interleukin 1 receptor antagonist (IL-1RN) Kit is a sandwich ELISA. The capture antibody is a polyclonal human IL-1RN antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known IL-1RN concentration are added to these wells and allowed to complex with the bound IL-1RN antibody. A biotinylated human polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1RN present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IL-1RN in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, R26-E177
Bosters human IL-1RA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1RA has been precoated onto 96-well plates. Standards(E.coli, R26-E177) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1RA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-1RA amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Kallikrein-3 Kit is a sandwich ELISA. The capture antibody is a polyclonal human Kallikrein-3 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known Kallikrein-3 concentration are added to these wells and allowed to complex with the bound Kallikrein-3 antibody. A biotinylated human polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Kallikrein-3 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Kallikrein-3 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The antigen and standard used in this Kallikrein-3 ELISA is recombinant human Kallikrein-3 amino acids 18-261 produced in and purified from a mammalian cell expression system.
Bosters human Kallikrein 3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kallikrein 3 has been precoated onto 96-well plates. Standards(NSO, A18-P261) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kallikrein 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Kallikrein 3 amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The rat TrkA Kit is a sandwich ELISA. The capture antibody is a polyclonal rat TrkA antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known TrkA concentration are added to these wells and allowed to complex with the bound TrkA antibody. A biotinylated rat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TrkA present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat TrkA in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Rat
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A33-P418
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Interleukin-23 subunit alpha (IL-23-A) Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse IL-23-A antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known IL-23-A concentration are added to these wells and allowed to complex with the bound IL-23-A antibody. A biotinylated mouse monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-23-A present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse IL-23-A in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from an insect expression system consisting of amino acids (M23-S335)+(L20-A196)
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The Hepatitis A virus cellular receptor 1 homolog is also known as the Kidney injury molecule 1 (KIM1) protein. The mouse KIM1 Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse KIM1 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known KIM1 concentration are added to these wells and allowed to complex with the bound KIM1 antibody. A biotinylated mouse monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of KIM1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse KIM1 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids Y22-T212
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Hepatitis A virus cellular receptor 1 homolog; HAVcr-1; Kidney injury molecule 1; KIM-1; T cell immunoglobulin and mucin domain-containing protein 1; TIMD-1; T cell membrane protein 1; TIM-1; Havcr1; Kim1; Tim1; Timd1;
Accession Number:
Q5QNS5 HAVR1_MOUSE;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Heat shock protein beta-1 (HSPB1) Kit is a sandwich ELISA. The capture antibody is a monoclonal human HSPB1 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known HSPB1 concentration are added to these wells and allowed to complex with the bound HSPB1 antibody. A biotinylated human monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of HSPB1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human HSPB1 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
Heat shock protein beta-1 (HSPB1) belongs to the small heat shock protein (HSP20) family. HSPB1 is expressed in response to heat shock and is also activated by estrogen in hormone responsive cells. HSPB1 is expressed in most cells with highest expression in heart and in tissues composed of striated and smooth muscle.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, T2-K205
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The Hepatitis A virus cellular receptor 1 homolog is also known as the Kidney injury molecule 1 (KIM1) protein. The rat KIM1 Kit is a sandwich ELISA. The capture antibody is a polyclonal rat KIM1 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known KIM1 concentration are added to these wells and allowed to complex with the bound KIM1 antibody. A biotinylated rat monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of KIM1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat KIM1 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Rat
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids S18-V238
Bosters rat KIM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KIM1 has been precoated onto 96-well plates. Standards(NSO, S18-V238) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KIM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat KIM1 amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Hepatitis A virus cellular receptor 1 homolog; HAVcr-1; Kidney injury molecule 1; KIM-1; T cell immunoglobulin and mucin domain-containing protein 1; TIMD-1; T cell membrane protein 1; TIM-1; Havcr1; Kim1; Tim1; Timd1;
Accession Number:
O54947 HAVR1_RAT;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human Platelet endothelial cell adhesion molecule or CD31 Kit is a sandwich ELISA. The capture antibody is a polyclonal human CD31 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known CD31 concentration are added to these wells and allowed to complex with the bound CD31 antibody. A biotinylated human polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD31 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human CD31 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids Q28-K601
Bosters human PECAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PECAM-1 has been precoated onto 96-well plates. Standards(CHO, Q28-K601) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PECAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PECAM-1 amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Platelet endothelial cell adhesion molecule or CD31 Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse CD31 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known CD31 concentration are added to these wells and allowed to complex with the bound CD31 antibody. A biotinylated mouse polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD31 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse CD31 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids E18-K590
Bosters mouse PECAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PECAM-1 has been precoated onto 96-well plates. Standards(NSO, E18-K590) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PECAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse PECAM-1 amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The mouse Oxidized low-density lipoprotein receptor 1 (Ox-LDL receptor 1) Kit is a sandwich ELISA. The capture antibody is a monoclonal anti-mouse Ox-LDL receptor 1 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known Ox-LDL receptor 1 concentration are added to these wells and allowed to complex with the bound Ox-LDL receptor 1 antibody. A biotinylated anti-mouse polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Ox-LDL receptor 1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Ox-LDL receptor 1 in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Mouse
Immunogen:
The antigen and standard used in this Oxidized low-density lipoprotein receptor 1 ELISA is recombinant mouse LOX-1/OLR1 amino acids 60-363 produced in and purified from a mammalian cell expression system.
Bosters mouse LOX-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for LOX-1 has been precoated onto 96-well plates. Standards(NSO, R60-I363) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LOX-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse LOX-1 amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human p53 Kit is a sandwich ELISA. The capture antibody is a polyclonal human p53 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known p53 concentration are added to these wells and allowed to complex with the bound p53 antibody. A biotinylated human polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of p53 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human p53 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
p53 is a DNA-binding protein containing transcription activation, DNA-binding and oligomerization domains. p53 is ubiquitously expressed and responds to a variety of cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair or changes in metabolism. Multiple p53 variants are produced from alternative promoters and alternative splicing.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids M1-D393
Bosters human P53 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for P53 has been precoated onto 96-well plates. Standards(NSO, M1-D393) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P53 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human P53 amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Human p53
Assay Range:
156 pg/mL - 10000 pg/mL
Sensitivity:
< 10 pg/mL
References:
Liu ZH et al. (2014) Prussian blue-doped nanogold microspheres for enzyme-free electrocatalytic immunoassay of p53 protein. Microchimica Acta, Apr 2014; 181(5): 581-8
The rat Interleukin-13 (IL-13) Kit is a sandwich ELISA. The capture antibody is a monoclonal anti-rat IL-13 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known IL-13 concentration are added to these wells and allowed to complex with the bound IL-13 antibody. A biotinylated anti-rat antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-13 present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat IL-13 in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Rat
Immunogen:
The standard and immunogen in this assays is produced in and purified from an E.coli expression system consisting of amino acids, T19-H131
Bosters rat IL-13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-13 has been precoated onto 96-well plates. Standards(E.coli, T19-H131) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-13 amount of sample captured in plate.
Application Details:
Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
IL-13; T-cell activation protein P600; IL13;
Accession Number:
P42203 IL13_RAT;
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The human TrkA Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse TrkA antibody pre-coated onto the 96-well strip plates provided in the kit. Standards of known TrkA concentration and test samples are added to these wells and allowed to complex with the bound TrkA antibody. A biotinylated polyclonal detection antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TrkA present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TrkA in samples such as culture supernates, cell lysates and tissue homogenates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4C
Species Reactivity:
Human
Immunogen:
The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids A33-E407
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
The Human TrkB Kit is a sandwich ELISA. The capture antibody is a TrkB antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TrkB concentration are added to these wells and allowed to complex with the bound TrkB antibody. A biotinylated TrkB antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TrkB present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TrkB in samples such as cell lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
TrkB is a member of the neurotrophic tyrosine receptor kinase family. It is a membrane-bound receptor and upon neurotrophin binding, it phosphorylates itself as well as MAPK pathways members. TrkB is the receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). It is Involved in the development and/or maintenance of the nervous system.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen for this kit is recombinant TrkB produced in and purified from the NSO expression system.
Does not show cross-reactivity with other neurotrophin receptors
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of human TrkB in samples such as cell lysates and cell culture supernates Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The human TREM2 kit is a sandwich ELISA. The capture antibody is a mouse monoclonal TREM2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TREM2 concentration are added to these wells and allowed to complex with the bound TREM2 antibody. A biotinylated goat polyclonal TREM2 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TREM2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TREM2 in samples such as sera, plasma (heparin, EDTA), and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
The protein Triggering receptor expressed on myeloid cells 2 (TREM2) forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein (TYROBP). TREM2 has roles in the immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding isoforms with differing subcellular locations.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen for this kit is recombinant TREM2 protein produced in and purified from an E.coli expression system using immunogen sequence H19-E167.
ELISA. The purpose of this kit is the in-vitro quantitative determination of human TREM2 in samples such as sera, plasma (heparin, EDTA), and cell culture supernates. Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Triggering receptor expressed on myeloid cells 2; TREM-2; Triggering receptor expressed on monocytes 2; TREM2
Accession Number:
Q9NZC2 TREM2_HUMAN
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
The Human FGF1 ELISA is a solid phase immunoassay specifically designed to measure Human FGF1. The capture antibody is a mouse monoclonal FGF1 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known FGF1 concentration are added to these wells and allowed to complex with the bound FGF1 antibody. A biotinylated FGF1 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of FGF1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human FGF1 in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin and EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures
Background Info:
FGF1 is important in cell survival, cell division, angigenesis, cell differentiation and cell migration.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen for this kit is recombinant Human FGF1 produced in E.coli cells; Immunogen sequence: A2-D155.
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of Human FGF1 in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin and EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Fibroblast growth factor 1 (FGF1)
Accession Number:
P05230FGF1 HUMAN
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, human FGF1 protein standards, human FGF1 biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample diluent, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
The Human FGF4 Enzyme-Linked Immunosorbent Assay kit (ELISA), is a sandwich ELISA. The capture antibody is a mouse monoclonal FGF4 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known FGF4 concentration are added to these wells and allowed to complex with the bound FGF4 antibody. A biotinylated FGF4 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of FGF4 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human FGF4 in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin and EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
FGF4 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiations. FGF4 is required for normal limb and cardic valve development during embryogensis (Uniprot).
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen for this kit is recombinant Human FGF4 produced in E.coli cells; Immunogen sequence: A31-L206
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of human FGF4 in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin and EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Fibroblast growth factor 4; FGF4
Accession Number:
P08620 FGF4_HUMAN
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, human FGF4 protein standards, human FGF4 biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample diluent, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
The Human FLT3LG ELISA kit is a sandwich ELISA. The capture antibody is a mouse monoclonal FLT3LG antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known FLT3LG concentration are added to these wells and allowed to complex with the bound n FLT3LG antibody. A biotinylated FLT3LG goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of FLT3LG present in the samples. The purpose of this kit is the in-vitro quantitative determination of Human FLT3LG in samples such as cell culture supernatants, serum and plasma (heparin, EDTA and Citrate). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
Fms-related tyrosine kinase 3 ligand (FLT3LG), is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor(SCF) and colony stimulating factor 1(CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors (Uniprot).
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen for this kit is recombinant human Flt3 Ligand expression system sf21; Immunogen sequence: T27-P185
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of human Flt3 Ligand in samples such as cell culture supernatants, serum and plasma (heparin, EDTA and Citrate). Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Fms-related tyrosine kinase 3 ligand; Flt3 Ligand
Accession Number:
P49771 FLT3L_HUMAN
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, human Flt3 Ligand protein standards, human Flt3 Ligand biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample diluent, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
The Mouse Flt3 Ligand ELISA kit is a sandwich ELISA. The capture antibody is a rat monoclonal FLT3LG antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known Mouse concentration are added to these wells and allowed to complex with the bound FLT3LG antibody. A biotinylated FLT3LG goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of FLT3LG present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse FLT3LG in cell culture supernatants and serum. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
Fms-related tyrosine kinase 3 ligand (FLT3LG), is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor(SCF) and colony stimulating factor 1(CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors (Uniprot).
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen for this kit is recombinant Mouse FLT3LG expression system NSO; Immunogen sequence: G27-R188
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of Mouse FLT3LG concentrations in cell culture supernates and serum Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Fms-related tyrosine kinase 3 ligand(FLT3LG), also known as Flt-3ligand or FL
Accession Number:
P49772 FLT3L_MOUSE
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, mouse FLT3LG protein standards, mouse FLT3LG biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample diluent, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
The Human IGF-1 receptor kit is a sandwich ELISA. The capture antibody is a mouse monoclonal IGF-1 receptor antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known IGF-1 receptor concentration are added to these wells and allowed to complex with the bound IGF-1 receptor antibody. A biotinylated IGF-1 receptor goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IGF-1 receptor present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IGF-1 receptor in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin and EDTA ). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
IGF-1 receptor binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF-1R is involved in cell growth and survival control. IGF-1 receptor is crucial for tumor transformation and survival of malignant cells.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen for this kit is recombinant Human IGF1R expressed in a NSO expression system; Immunogen sequence: E31-N932
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of Human IGF-1 receptor in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin and EDTA ) Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The Human OLR1 ELISA kit is a sandwich ELISA. The capture antibody is a mouse monoclonal OLR1 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known OLR1 concentration are added to these wells and allowed to complex with the bound OLR1 antibody. A biotinylated OLR1 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of OLR1F present in the samples. The purpose of this kit is the in-vitro quantitative determination of human OLR1 in samples such as cell culture supernatants, serum and plasma (heparin, EDTA and Citrate). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
LOX-1/OLR1, is a receptor protein that in humans is encoded by the OLR1 gene. LOX-1 mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis (Uniprot).
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen for this kit is recombinant human OLR1. The expression system is NSO; Immunogen sequence: S61-Q273
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of human OLR1 in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin and EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The human Renin kit is a sandwich ELISA. The capture antibody is a mouse monoclonal human Renin antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known human Renin concentration are added to these wells and allowed to complex with the bound human Renin antibody. A biotinylated human Renin goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Renin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Renin in samples such as cell culture supernatants, serum, plasma (heparin, EDTA) and urine. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen for this kit is recombinant Human Renin expressed in a NSO expression system; Immunogen sequence: L67-R406
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of Human Renin protein in samples such as cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Angiotensinogenase
Accession Number:
P00797 RENI_HUMAN
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, Human Renin protein standards, Human Renin biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
The mouse Renin-1 kit is a sandwich ELISA. The capture antibody is a rat monoclonal mouse Renin-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known mouse Renin-1 concentration are added to these wells and allowed to complex with the bound mouse Renin-1 antibody. A biotinylated mouse Renin-1 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of mouse Renin-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Renin-1 in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen for this kit is recombinant Mouse Renin-1 expressed in a NSO expression system; Immunogen sequence: S72-R402
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of Mouse Renin-1 protein in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Angiotensinogenase, Kidney renin
Accession Number:
P06281 RENI1_MOUSE
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, Mouse Renin-1 protein standards, Mouse Renin-1 biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
The human Persephin kit is a sandwich ELISA. The capture antibody is a human Persephin antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known human Persephin concentration are added to these wells and allowed to complex with the bound human Persephin antibody. A biotinylated human Persephin antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of human Persephin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Persephin in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
Persephin exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen for this kit is recombinant human Persephin expressed in an E.coli expression system; Immunogen sequence: W22-G156
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of Human Persephin protein in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
PSP
Accession Number:
O60542 PSPN_HUMAN
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, human Persephin protein standards, human Persephin biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
The human Artemin kit is a sandwich ELISA. The capture antibody is a mouse monoclonal antibody to human Artemin pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known human Artemin concentration are added to these wells and allowed to complex with the bound human Artemin antibody. A biotinylated polyclonal goat antibody to human Artemin is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of human Artemin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Artemin in samples such as cell culture supernatants, serum and plasma (heparin, EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
Artemin is a ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. It supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen for this kit is recombinant human Artemin expressed in an E.coli expression system; Immunogen sequence: A108-G220
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of human Artemin protein in samples such as cell culture supernatants, serum and plasma (heparin, EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Enovin, Neublastin
Accession Number:
Q5T4W7 ARTN_HUMAN
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, human Artemin protein standards, human Artemin biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
The mouse Artemin kit is a sandwich ELISA. The capture antibody is a rat monoclonal antibody to mouse Artemin pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known mouse Artemin concentration are added to these wells and allowed to complex with the bound antibody to mouse Artemin. A biotinylated polyclonal goat antibody to mouse Artemin is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of mouse Artemin present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse Artemin in samples such as cell culture supernatants, serum and plasma (heparin, EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
Artemin is a ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. It supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen for this kit is recombinant mouse Artemin expressed in an E.coli expression system; Immunogen sequence: A112-G224
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of mouse Artemin protein in samples such as cell culture supernatants, serum and plasma (heparin, EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
Artemin
Accession Number:
Q9Z0L2 ARTN_MOUSE
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, mouse Artemin protein standards, mouse Artemin biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
The human CDNF kit is a sandwich ELISA. The capture antibody is a mouse monoclonal antibody to human CDNF pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known human CDNF concentration are added to these wells and allowed to complex with the bound antibody to human CDNF. A biotinylated polyclonal goat antibody to human CDNF is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of human CDNF present in the samples. The purpose of this kit is the in-vitro quantitative determination of human CDNF in samples such as cell culture supernatants, serum and plasma (heparin, EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
CDNF is a trophic factor for dopamine neurons. It prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen for this kit is recombinant human CDNF expressed in an E.coli expression system; Immunogen sequence: Q25-L187
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of human CDNF protein in samples such as cell culture supernatants, serum and plasma (heparin, EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
Alternative Names:
CDNF
Accession Number:
Q49AH0 CDNF_HUMAN
Kit Components:
The ELISA kit box contains 1 x 96-well pre-coated strip plate, human CDNF protein standards, human CDNF biotinylated antibody, Avidin-Biotin-Peroxidase Complex, sample, antibody and avidin-biotin peroxidase diluents, substrate reagent, stop solution, wash buffer, plate sealers and detailed protocols.
The Mouse CDNF ELISA kit is a sandwich ELISA. The capture antibody is a rat monoclonal CDNF antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known CDNF concentration are added to these wells and allowed to complex with the bound CDNF antibody. A biotinylated CDNF goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CDNF present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse CDNF in samples such as cell culture supernatants, serum and plasma (heparin and EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
CDNF (conserved dopamine neurotrophic factor) is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007; Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson's disease. When administered after 6-OHDA or MPTP -lesioning, it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard and immunogen for this kit is recombinant mouse CDNF produced in E.coli cells; Immunogen sequence: Q25-L187
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of mouse CDNF in samples such as cell culture supernatants, serum and plasma (heparin, EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The Mouse TrkC ELISA kit is a sandwich ELISA. The capture antibody is a rat monoclonal TrkC antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known TrkC concentration are added to these wells and allowed to complex with the bound TrkC antibody. A biotinylated TrkC goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TrkC present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse TrkC in samples such as cell culture supernatants and cell lysates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
TrkC is a member of the neurotrophic tyrosine receptor kinase family. TrkC is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC is the receptor for neurotrophin-3 (NT-3).Signalling through TrkC leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 4 named isoforms produced by alternative splicing. Additional isoforms seem to exist. Mutations in TrkC have been associated with medulloblastomas, secretory breast carcinomas and other cancers.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard for this kit is recombinant mouse TrkC produced in NS0 cells; Immunogen sequence: C32-T429
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of mouse TrkC in samples such as cell culture supernatants and cell lysates. Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The Rat TrkC ELISA kit is a sandwich ELISA. The capture antibody is a mouse monoclonal TrkC antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known TrkC concentration are added to these wells and allowed to complex with the bound TrkC antibody. A biotinylated TrkC goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TrkC present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat TrkC in samples such as cell culture supernatants and cell lysates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
TrkC is a member of the neurotrophic tyrosine receptor kinase family. TrkC is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC is the receptor for neurotrophin-3 (NT-3). Signalling through TrkC leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 4 named isoforms produced by alternative splicing. Additional isoforms seem to exist. Mutations in TrkC have been associated with medulloblastomas, secretory breast carcinomas and other cancers.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard for this kit is recombinant rat TrkC produced in NS0 cells; Immunogen sequence: C32-T429
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of rat TrkC in samples such as cell culture supernatants and cell lysates. Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The Rat CD44 ELISA kit is a sandwich ELISA. The capture antibody is a mouse monoclonal CD44 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known CD44 concentration are added to these wells and allowed to complex with the bound CD44 antibody. A biotinylated CD44 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD44 present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat CD44 in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
CD44 protein is also known as the receptor for hyaluronic acid (HA). CD44 mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, CD44 may play an important role in invadopodia formation, and it also is involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction of CD44 causes numerous pathogenic phenotypes. CD44 has great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard for this kit is recombinant rat CD44 produced in NS0 cells; Immunogen sequence: Q22-E271
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of rat CD44 in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The Mouse CD44 ELISA kit is a sandwich ELISA. The capture antibody is a rat monoclonal CD44 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known CD44 concentration are added to these wells and allowed to complex with the bound CD44 antibody. A biotinylated CD44 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD44 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse CD44 in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
CD44 protein is also known as the receptor for hyaluronic acid (HA). CD44 mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, CD44 may play an important role in invadopodia formation, and it also is involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction of CD44 causes numerous pathogenic phenotypes. CD44 has great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard for this kit is recombinant mouse CD44 produced in CHO cells; Immunogen sequence: Q25-T224
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of mouse CD44 in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The Human CD44 ELISA kit is a sandwich ELISA. The capture antibody is a goat polyclonal CD44 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known CD44 concentration are added to these wells and allowed to complex with the bound CD44 antibody. A biotinylated CD44 goat polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD44 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human CD44 in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
CD44 protein is also known as the receptor for hyaluronic acid (HA). CD44 mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, CD44 may play an important role in invadopodia formation, and it also is involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction of CD44 causes numerous pathogenic phenotypes. CD44 has great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C
Species Reactivity:
Human
Immunogen:
The standard for this kit is recombinant human CD44 produced in NS0 cells; Immunogen sequence: Gln21 - Pro220
There is no detectable cross-reactivity with other relevant proteins.
Application Details:
ELISA. The purpose of this kit is the in-vitro quantitative determination of human CD44 in samples such as cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.
The human fibroblast growth factor 2 (FGF2) Kit is a sandwich ELISA. The capture antibody is a human FGF2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known FGF2 concentration are added to these wells and allowed to complex with the bound FGF2 antibody. A biotinylated human FGF2 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of FGF2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human FGF2 in samples such as cell culture supernatant, serum and plasma (EDTA, heparin). This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human, There is no detectable cross-reactivity with other relevant proteins.
Immunogen:
Standard and antigen for this kit is recombinant human FGF-2, amino acids 143-288, expressed in and purified from E.coli.
KDEL (lys-asp-glu-leu) is a C-terminal protein involved in the endoplasmatic reticulum retention system and appears to be sufficient to reduce secretion from the ER.This antibody is directly conjugated to HRP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat. Detects KDEL proteins, GRP94, Grp78, PDI and calreticulin. It may also see ERp57 and ERp29.
Immunogen:
KDEL containing peptide
Applications:
Immunohistochemistry, paraffin-embedded (IHC), Western blot (WB)
Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (bronchoalveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.
The EV-Spinner is a 2.5 ml non-stick ultrafiltration (UF) concentrator designed to enhance the recovery of extracellular vesicle (EVs) during the concentration or buffer exchange step.
The ultrafiltration works in the opposite direction to the centrifugal force, providing higher particle recovery and ensuring the filter does not clog.
The low protein binding membrane (made of polyethersulphone) means much lower loss of extracellular vesicles, compared to standard V-shape concetrators.
Maximum sample volume: 2.5 ml
Centrifugation speed: 2500 g Swing bucket rotor / 2000 g Fixed angle rotor
Application Details:
Applications: Concentration of solutions containing EVs, exosomes or nanoparticles. Concentration of diluted matrices as cell media or urine prior to EV isolation. Removal of small molecules and unbound dyes from solution containing EVs/exosomes and nanoparticles. EV and exosomes dialysis. Concentration of EV suspensions post SEC isolation.
Diaz-Romero, J. et al. (2008) Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather amplification of progenitor cells. J Cell Physiol. 214: 75-83
Chen HL, Demiralp B, Schneider A, et al. Parathyroid hormone and parathyroid hormone-related protein exert both pro- and anti- apoptotic effects in mesenchymal cells. Journal of Biology and Chemistry 2002; 277:19374-81.
HOT FIREPol® SolisGreen qPCR Mix is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating SolisGreen dye. It comprises all the components necessary, excluding the template and primers, to perform highly sensitive qPCR. The user simply needs to add water, template and primers.
HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. Suitable for ROX-dependent and ROX-independent qPCR cyclers
Product Type:
DNA Polymerase qPCR Mix
Storage Temp:
Routine storage: -20 ºC
Applications:
> Detection and quantification of DNA and cDNA targets > Profiling gene expression > Microbial detection > Viral load determination
HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C for 1 year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Antibody in concentration 1 µg/ml was sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7. For western blot results, immunofluorescence and immunogold images please referr to Napier et al. 1992.
Clone number:
IgG2B
Application Details:
1: 50-1 : 500 (IF), 1 : 100-1. 1000 (WB)
Purity:
Protein G purified
Related products:
AS10 683-25 | Anti-HDEL | endoplasmic reticulum retention signal mouse antibody, 25 µgAS13 2665 | Anti-KDEL | endoplasmic reticulum retention signal (50 µg), rabbit antibodiescollection of antibodies for endomembrane proteinsPlant and algal protein extraction bufferSecondary antibodies
Molecular Weight:
78 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Luo et al. (2006). GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol Cell Biol. 26(15): 5688-5697.Napier et al. (1992). Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. J Cell Sci. 102: 261-271.
Special application note:
Protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 1 mg/ml.
Oxidative derivate of guanosine is called 8-Hydroxyguanosine (8OHdG) and is used as a popular biomarker of oxidative stress.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
Recognizes markers of oxidative damage to DNA (8-hydroxy-2’-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine)
Immunogen:
8-hydroxy-guanosine-BSA and – casein conjugates
Applications:
ELISA (ELISA), Immunoaffinity chromatography (IAP), Immunohistochemistry on frozen tissue and paraffin-embedded (IHC-Fr-P)
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Poborilova et al. (2015). DNA hypomethylation concomitant with the overproduction of ROS induced by naphthoquinone juglone on tobacco BY-2 suspension cells. Environmental and Experimental Botany, Volume 113, May 2015, Pages 28–39.Haigh and Drew (2015). Cavitation during the protein misfolding cyclic amplification (PMCA) method - The trigger for de novo prion generation? Biochem Biophys Res Commun. 2015 Apr 17. pii: S0006-291X(15)00726-3. doi: 10.1016/j.bbrc.2015.04.048.
Special application note:
Protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 0.65 mg/ml.
Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine antibodies are helpful in facilitating the identification of tyrosine kinase substrates.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C for one year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
Antibody reacts with phosphotyrosine and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysated. Does not cross react with phosphoserine or phosphothreonine.
Immunogen:
Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin KLH with 1-ethyl-3-(3’-dimentrylaminopropyl) carbodiimide
Applications:
Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
1 µg/ml of this antibody is sufficient for detection of phosphorylated tyrosine residues in 10 µg of rat tissue lysate by colorimetric immunoblot analysis.
Clone number:
IgG1
Application Details:
1 : 1000 (WB)
Purity:
Protein G purified
Related products:
Plant and algal protein extraction buffer Secondary antibodies
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Garton & Tonks (1999). Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST. J Biol Chem 6:3811-3818.Tiganis et al. (1999). The protein-tyrosine phosphatase TCPTP regulates epidermal growth factor receptor-mediated and phosphatidylinositol 3-kinase-dependent signaling. J Biol Chem 39: 27768-27775.(IF):Garton et al. (1996). Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST. Mol and Cell Bio 11:6408-6418.(IP):
Special application note:
Protein G purified IgG1 in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 1 mg/ml.
Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species. Protein tyrosine nitration results in a post-translational modification, component of nitric oxide signaling.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivitywith non-nitrated tyrosine. Not species specific.0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot..ECL.Antibody works paraffin-embedded sections.
Clone number:
IgG2A
Application Details:
1: 100 (IHC), 1: 1400 (WB). The exact and optimal working dilution should be determined by the investigator.
Purity:
Protein G purified, in PBS with 50 % glycerol, 0.09% sodium azide.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.Antibody used in immunohistochemistry:Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387.
Special application note:
1 mg/ml of Protein G purified IgG2A in PBS pH 7.4, 0.09 % sodium azide, 50 % glycerol
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
Bovine, avian
Expected Species:
Higher plants
Immunogen:
acetylated KLH
Applications:
ELISA (ELISA), Immunocytochmistry/Immunofluorescence (ICC/IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
1 µg of this antibody is sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein and ECL detection system
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Vigushin & Coombes (2004). Targeted histone deacetylase inhibition for cancer therapy. Curr. Cancer Drug Targets 4: 205-218.
Special application note:
Protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 1 mg/mlantibody detects Proteins containing acetylated lysine residues in ELISA and WBs. Does not detect non-acetylated lysine residues.
DMPO (5,5-dimethly-1-proline N-oxide) is a compound used in spin trapping. It is a method employed in chemistry as an analytical technique for detection and identification of short-lived free radicals. DMPO adducts can be generated with protein and DNA radicals.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C for one year. once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
DMPO (species independent)
Expected Species:
DMPO (species independent)
Immunogen:
5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to ovalbumin
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immuno-spin trapping (IT), Immunoprecipitation (IP), Western blot (WB)
collection of antibodies to proteins involved in oxidative stressSecondary antibodies
Molecular Weight:
90 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Chatterjee et al. (2009). Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice. Free Radic. Med. and Biol. 46:454-461.
Special application note:
Antibody is purified on Protein G and present in TBS pH 7.4 with 0.05 % sodium azide as preservative and 50 % glycerol.DMPO antibody in dilution of 1: 1000 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colormetric immunoblot analysis using goat anti-mouse IgG HRP conjugated secondary antibody.
Hellebrin is a plant cardiotonic glycoside which presents a bufadienolide-steroid structure. It represents a potent inhibitor of sodium pump blocking the active efflux of Na+ and reuptake of K+ of this membrane enzyme. Hellebrin is a strong immunosuppressor inhibiting T cell activity with much higher potency than cortisol or cyclosporin A.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4°C. Long-term -20°. Repeated freezing and thawing is not recommended. Solution contains 0.01% sodium azide.
Host Animal:
Rabbit
Species Reactivity:
Helleborus niger
Immunogen:
ovalbumin-conjugated hellebrin extracted from Helleborus niger
Aflatoxins are naturally occurring mycotoxins that are produced by many species of Aspergillus. Aflatoxins are toxic and among the most carcinogenic substances known. Crops which are frequently affected by Asperiillus include cereals (maize, sorghum, pearl millet, rice, wheat), oilseeds (peanut, soybean, sunflower, cotton), spices (chile peppers, black pepper, coriander, turmeric, ginger), and tree nuts (almond, pistachio, walnut, coconut, brazil nut).
KDEL (lys-asp-glu-leu) is a C-terminal protein involved in the endoplasmatic reticulum retention system and appears to be sufficient to reduce secretion from the ER.This antibody is directly conjugated to FITC.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat. Detects KDEL proteins, GRP94, Grp78, PDI and calreticulin. It may also see ERp57 and ERp29.
Immunogen:
KDEL containing peptide
Applications:
Immunohistochemistry, paraffin-embedded (IHC), Western blot (WB)
Arginylation is a post-translational modification of an existing peptide chain by addition of an extra arginine. This modification changes primary sequence of protein as well as it's surface change. It is mediated by arginyltransferase ATE1. Arginylation plays an essential role in multiple physiological pathways, for example, in vivo arginylation constitutes a mechanism for degradation of preprocessed proteins or proteolytic fragments that bear Asp and Glu on their N-termini.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
MG132 or epoxomycin are recommended to use to inhibit proteasome and significantly increase signal from the arginylated proteins.
Application Details:
1 : 1000 (Dot)
Purity:
Affinity purified serum in PBS, pH 7.4
Reconstitution:
For reconstitution add 50 µl of sterile water to each tube
Related products:
AS15 3050A | Anti-RD | N-terminal arginylation, rabbit antibodies collection of antibodies to proteins involved in transcriptioncollection of antibodies to proteins involved in translationPlant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Special application note:
Antibody is purified using substractive purification method.
Arginylation is a post-translational modification of an existing peptide chain by addition of an extra arginine. This modification changes primary sequence of protein as well as it's surface change. It is mediated by arginyltransferase ATE1. Arginylation plays an essential role in multiple physiological pathways, for example, in vivo arginylation constitutes a mechanism for degradation of preprocessed proteins or proteolytic fragments that bear Asp and Glu on their N-termini.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 25 µl of sterile water to each tube.
Related products:
AS15 3050 | anti-RE | N-terminal arginylation, rabbit antibodies collection of antibodies to proteins involved in transcriptioncollection of antibodies to proteins involved in translationPlant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Special application note:
Antibodies are purified using substactive purification method. MG132 or epoxomycin are recommended to use to inhibit proteasome and significantly increase signal from the arginylated proteins.For exact protocol of dot blot and SPOT assay, please inquire.
Mouse anti-Human IgG1 Fc, HRP conjugated is a secondary antibody which binds to human IgG1 FC part in immunological assays.
Product Type:
Antibody
Antibody Type:
Monoclonal
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Reconstituted material can be stoored in +4°C up to 7 days.
Host Animal:
Mouse
Species Reactivity:
human IgG1
Immunogen:
Purified monoclonal IgG1 isolated from pooled human serum.
for reconstitution please add 0.5 ml of sterile destilled water.
Related products:
Secondary antibodies
Not reactive in:
The antiserum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Mouse anti-HLA-Class I, clone W6/32, Biotin (Monoclonal)
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
concentrate
Storage Temp:
2-8°C
Host Animal:
Mouse
Immunogen:
Membrane of human tonsil cells
Applications:
C
Clone number:
W6/32
Antibody Isotype:
Mouse IgG2a
Monosan Brand:
Monosan
Concentration:
1 mg/ml
Conjugate:
Biotin
Specificity:
HLA-Class I
References:
Jacobsen, C. N. et al. (1993): Reactivities of 20 anti-human monclonal antibodies with leucocytes from ten different animal species. Vet. Immunopathol. 39: 461 -466
Mouse anti-HLA-Class I, clone W6/32, FITC (Monoclonal)
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
concentrate
Storage Temp:
2-8°C
Host Animal:
Mouse
Immunogen:
Membrane of human tonsil cells
Applications:
C
Clone number:
W6/32
Antibody Isotype:
Mouse IgG2a
Monosan Brand:
Monosan
Concentration:
1 mg/ml
Conjugate:
FITC
Specificity:
HLA-Class I
References:
Jacobsen, C. N. et al. (1993): Reactivities of 20 anti-human monclonal antibodies with leucocytes from ten different animal species. Vet. Immunopathol. 39: 461 -466
Mouse anti-HLA-Class I, clone W6/32, PE (Monoclonal)
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
concentrate
Storage Temp:
2-8°C
Host Animal:
Mouse
Immunogen:
Membrane of human tonsil cells
Applications:
C
Clone number:
W6/32
Antibody Isotype:
Mouse IgG2a
Monosan Brand:
Monosan
Concentration:
1 mg/ml
Conjugate:
PE
Specificity:
HLA-Class I
References:
Jacobsen, C. N. et al. (1993): Reactivities of 20 anti-human monclonal antibodies with leucocytes from ten different animal species. Vet. Immunopathol. 39: 461 -466
Flenghi, L. et al. (1990) LF61: a new monoclonal antibody directed against a trimeric molecule (150kDa, 125kDa, 105kDa) associated with hairy cell leukaemia. Br. J. Haem. 76: 451-459
Zhang, W. et al. (2000). Neutrophil lactoferrin release induced by IgA immune complexes differed from that induced by cross-linking of Fc alpha receptors (Fc alpha R) with a monoclonal antibody, MIP8a. Clin. Exp. Immunol. 121: 106-111
Affinity purified antibody is > 95% based on SDS-PAGE
Host:
Goat
Immunogen:
Purified Rat IgG, whole molecule
Buffer:
PBS, 1% BSA & 0.1% proclin 150
Storage:
2-8 °C
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Specificity:
Based on IEP, this immunopolymer antibody reacts with heavy (?) chains on rat IgG, light chains on all rat immunoglobulins
Cross Reactivity:
Based on IEP, no cross-reactivity is observed to non-immunoglobulin rat serum proteins, IgG from human or mouse
Country Of Origin:
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Lilliehook, I. et al. (1998). Expression of adhesion and Fc gamma - receptors on canine blood eosinophils and neutophils studied by anti-human monoclonal antibodies. Vet. Immunol. Immunopathol. 61: 181 - 193