A ready-to-use 5X-concentrated solution for High Resolution Melt (HRM) analysis of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection. The mix has two versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
excellent resolution in HRM assays allows detection of DNA sequence variations contains sensitive EvaGreen® dye compatible with most cyclers different product versions: no ROX, ROX reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2, 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Bovine serum albumin (BSA) to enhance qPCR reaction
A ready-to-use 5X-concentrated solution for High Resolution Melt (HRM) analysis of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection. The mix has two versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
excellent resolution in HRM assays allows detection of DNA sequence variations contains sensitive EvaGreen® dye compatible with most cyclers different product versions: no ROX, ROX reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2, 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Bovine serum albumin (BSA) to enhance qPCR reaction
A ready-to-use 5X-concentrated solution for High Resolution Melt (HRM) analysis of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection. The mix has two versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
excellent resolution in HRM assays allows detection of DNA sequence variations contains sensitive EvaGreen® dye compatible with most cyclers different product versions: no ROX, ROX reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2, 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Bovine serum albumin (BSA) to enhance qPCR reaction
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12.5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Bovine serum albumine (BSA) to enhance qPCR reaction
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12.5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Bovine serum albumine (BSA) to enhance qPCR reaction
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12.5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Bovine serum albumine (BSA) to enhance qPCR reaction
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12.5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Bovine serum albumine (BSA) to enhance qPCR reaction
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Internal reference based on ROX dye
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Internal reference based on ROX dye
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Internal reference based on ROX dye
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Internal reference based on ROX dye
High performing probe-based qPCR Mix for AT-rich, regular and GC-rich templates.
Background Info:
A universal ready-to-use 5X reaction mix for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction with excellent amplification of GC-rich regions. The mix contains dUTPs to prevent carry-over contamination when used with UNG treatment, and an internal reference based on ROX dye and is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization. The mix is not compatible with Probes detected in ROX/JUN/Texas Red channel.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
suitable for assays with 1-2 targets high specificity and sensitivity superior results with templates with up to 75% GC content contains dUTP to prevent cross-contamination when used with UNG one qPCR mix for all cyclers (except capillary) reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
5x Probe Universal qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
dNTPs including dUTP: Mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Probe Universal qPCR Mix
Reference dye based on ROX: For multiplex application - if ROX dye is used as one of the fluorophores, the internal reference might interfere with the signal.
In separate vial
100% DMSO is included in the kit in a separate vial. DMSO is recommended as a PCR additive for templates with high GC content. In some cases, DMSO is also required to relax secondary structures.
Vials included in the kit:
HOT FIREPol® Probe Universal qPCR Mix 1x20 ml | 5000 rxn DMSO 100% 1 10 ml
High performing probe-based qPCR Mix for AT-rich, regular and GC-rich templates.
Background Info:
A universal ready-to-use 5X reaction mix for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction with excellent amplification of GC-rich regions. The mix contains dUTPs to prevent carry-over contamination when used with UNG treatment, and an internal reference based on ROX dye and is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization. The mix is not compatible with Probes detected in ROX/JUN/Texas Red channel.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
suitable for assays with 1-2 targets high specificity and sensitivity superior results with templates with up to 75% GC content contains dUTP to prevent cross-contamination when used with UNG one qPCR mix for all cyclers (except capillary) reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
5x Probe Universal qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
dNTPs including dUTP: Mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Probe Universal qPCR Mix
Reference dye based on ROX: For multiplex application - if ROX dye is used as one of the fluorophores, the internal reference might interfere with the signal.
In separate vial
100% DMSO is included in the kit in a separate vial. DMSO is recommended as a PCR additive for templates with high GC content. In some cases, DMSO is also required to relax secondary structures.
Vials included in the kit:
HOT FIREPol® Probe Universal qPCR Mix 1x0.2 ml | 50 rxn DMSO 100% 1x0.1 ml
High performing probe-based qPCR Mix for AT-rich, regular and GC-rich templates.
Background Info:
A universal ready-to-use 5X reaction mix for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction with excellent amplification of GC-rich regions. The mix contains dUTPs to prevent carry-over contamination when used with UNG treatment, and an internal reference based on ROX dye and is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization. The mix is not compatible with Probes detected in ROX/JUN/Texas Red channel.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
suitable for assays with 1-2 targets high specificity and sensitivity superior results with templates with up to 75% GC content contains dUTP to prevent cross-contamination when used with UNG one qPCR mix for all cyclers (except capillary) reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
5x Probe Universal qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
dNTPs including dUTP: Mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Probe Universal qPCR Mix
Reference dye based on ROX: For multiplex application - if ROX dye is used as one of the fluorophores, the internal reference might interfere with the signal.
In separate vial
100% DMSO is included in the kit in a separate vial. DMSO is recommended as a PCR additive for templates with high GC content. In some cases, DMSO is also required to relax secondary structures.
Vials included in the kit:
HOT FIREPol® Probe Universal qPCR Mix 1x8 ml | 2000 rxn DMSO 100% 1x4 ml
High performing probe-based qPCR Mix for AT-rich, regular and GC-rich templates.
Background Info:
A universal ready-to-use 5X reaction mix for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction with excellent amplification of GC-rich regions. The mix contains dUTPs to prevent carry-over contamination when used with UNG treatment, and an internal reference based on ROX dye and is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization. The mix is not compatible with Probes detected in ROX/JUN/Texas Red channel.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
suitable for assays with 1-2 targets high specificity and sensitivity superior results with templates with up to 75% GC content contains dUTP to prevent cross-contamination when used with UNG one qPCR mix for all cyclers (except capillary) reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
5x Probe Universal qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
dNTPs including dUTP: Mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Probe Universal qPCR Mix
Reference dye based on ROX: For multiplex application - if ROX dye is used as one of the fluorophores, the internal reference might interfere with the signal.
In separate vial
100% DMSO is included in the kit in a separate vial. DMSO is recommended as a PCR additive for templates with high GC content. In some cases, DMSO is also required to relax secondary structures.
Vials included in the kit:
HOT FIREPol® Probe Universal qPCR Mix 1x1 ml | 250 rxn DMSO 100% 1x0.5 ml
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
dNTPs: dATP, dCTP, dGTP and dTTP
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