Recombinant protein rDau c 1 is expressed in Escherichia coli. DNA sequence encoding 167 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 17,5 kDa.
Product Type:
Antibodies Primary
Storage Temp:
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Applications:
FC,ELISA
Additional Info:
The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
Recombinant protein rCup s 1 is expressed in Escherichia coli. DNA sequence encoding 359 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 39 kDa.
Storage Temp:
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Recombinant protein rCor a 1 is expressed in Escherichia coli. DNA sequence encoding 174 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 19,1 kDa.
Product Type:
Antibodies Primary
Storage Temp:
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Applications:
FC,ELISA
Additional Info:
The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
Recombinant protein rCan f 1 is expressed in Escherichia coli. DNA sequence encoding 169 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 18,8 kDa.
Product Type:
Antibodies Primary
Storage Temp:
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Applications:
FC,ELISA
Additional Info:
The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
Recombinant protein rBet v 1 is expressed in Escherichia coli. DNA sequence encoding 172 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 19 kDa.
Product Type:
Antibodies Primary
Storage Temp:
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Applications:
FC,ELISA
Additional Info:
The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
Recombinant protein rBet v 7 is expressed in Escherichia coli. DNA sequence encoding 186 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 19,7 kDa.
Product Type:
Antibodies Primary
Storage Temp:
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Applications:
ELISA,FC
Additional Info:
The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
Recombinant protein rBet v 6 is expressed in Escherichia coli. DNA sequence encoding 320 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 35,5 kDa.
Product Type:
Antibodies Primary
Storage Temp:
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Applications:
ELISA
Additional Info:
The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
Recombinant protein rBet v 4 is expressed in Escherichia coli. DNA sequence encoding 97 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 10,8 kDa.
Product Type:
Antibodies Primary
Storage Temp:
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Applications:
ELISA,FC
Additional Info:
The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
Recombinant protein rBet v 2 is expressed in Escherichia coli. DNA sequence encoding 152 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 16,2 kDa.
Product Type:
Antibodies Primary
Storage Temp:
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Applications:
ELISA,FC
Additional Info:
The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
This rat IgG2b kappa monoclonal antibody (clone RTG2B1-2, also known as G2B-1-2 or KLH/G2B-1-2) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.
Clone number:
RTG2B1-2
Antibody Isotype:
IgG2b
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Clone number:
RTG2B1-2
Antibody Isotype:
IgG2b
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
This rat IgG2b kappa monoclonal antibody (clone RTG2B1-2, also known as G2B-1-2 or KLH/G2B-1-2) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.
Clone number:
RTG2B1-2
Antibody Isotype:
IgG2b
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Additional Info:
This rat IgG2b kappa monoclonal antibody (clone RTG2B1-2, also known as G2B-1-2 or KLH/G2B-1-2) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.
Clone number:
RTG2B1-2
Antibody Isotype:
IgG2b
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
This rat IgG2a kappa monoclonal antibody (clone RTG2A1-1, also known as G2A-1-1 or KLH/G2A-1-1) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.
Clone number:
RTG2A1-1
Antibody Isotype:
IgG2a
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Additional Info:
This rat IgG2a kappa monoclonal antibody (clone RTG2A1-1, also known as G2A-1-1 or KLH/G2A-1-1) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.
Clone number:
RTG2A1-1
Antibody Isotype:
IgG2a
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
This rat IgG2a kappa monoclonal antibody (clone RTG2A1-1, also known as G2A-1-1 or KLH/G2A-1-1) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.
Clone number:
RTG2A1-1
Antibody Isotype:
IgG2a
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Clone number:
RTG2A1-1
Antibody Isotype:
IgG2a
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Additional Info:
This rat IgG2a kappa monoclonal antibody (clone RTG2A1-1, also known as G2A-1-1 or KLH/G2A-1-1) detects specifically the hemocyanin of keyhole limpet and can be used as a negative control antibody in other species.
Clone number:
RTG2A1-1
Antibody Isotype:
IgG2a
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Recombinant protein rAsp f 1 is expressed in Escherichia coli. DNA sequence encoding 150 AAs was fused with His-tag at the N-terminus. A calculated molecular mass of recombinant protein is 17 kDa.
Storage Temp:
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Recombinant protein rAra h 9 is expressed in Escherichia coli. DNA sequence encoding 105 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 10,6 kDa.
Product Type:
Antibodies Primary
Storage Temp:
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Recombinant protein rAra h 8 is expressed in Escherichia coli. DNA sequence encoding 169 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 18,3 kDa.
Product Type:
Antibodies Primary
Storage Temp:
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Applications:
ELISA
Additional Info:
The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
Recombinant protein rAra h 2 is expressed in Escherichia coli. DNA sequence encoding 164 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 19,5 kDa.
Product Type:
Antibodies Primary
Storage Temp:
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Recombinant protein rAra h 1 is expressed in Escherichia coli. DNA sequence encoding 614 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 70,2 kDa.
Product Type:
Antibodies Primary
Storage Temp:
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Applications:
ELISA,FC
Additional Info:
The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
Recombinant protein rApi m 2 is expressed in S2 cell (Drosophila). DNA sequence encoding 363 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 42,3 kDa.
Product Type:
Antibodies Primary
Storage Temp:
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Recombinant protein rApi m 1 is expressed in S2 cell (Drosophila). DNA sequence encoding 148 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 16,8 kDa.
Product Type:
Antibodies Primary
Storage Temp:
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Recombinant protein rApi g 1 is expressed in Escherichia coli. DNA sequence encoding 167 AAs was fused with His-tag at the C-terminus. A calculated molecular mass of recombinant protein is 17,9 kDa.
Product Type:
Antibodies Primary
Storage Temp:
Store at -20°C to -80°C.
Applications:
FC,ELISA
Additional Info:
The protein was purified by ionex and affinity chromatography, using His-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
Recombinant protein rAmb a 1 is expressed in Escherichia coli. DNA sequence encoding 384 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 41 kDa.
Storage Temp:
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Recombinant protein rAlt a 1 is expressed in Escherichia coli. DNA sequence encoding 152 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 16,7 kDa.
Product Type:
Antibodies Primary
Storage Temp:
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Applications:
FC,ELISA
Additional Info:
The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
PDK1 (3-phosphoinositide-dependent kinase 1), also known as PDPK1, is a key mediator of biological responses downstream of insulin and other tyrosine kinase receptors, regulating cell survival, cell cycle control, protein translation, and glucose metabolism. PDK1, containing a C-terminal pleckstrin homology domain, is activated in a phosphatidyl inositol 3,4,5-trisphosphate-dependent manner and phosphorylates multiple signaling molecules, including PKB/Akt, PKC, p70S6 kinase, serum and glucocorticoid-induced kinase. In T cells, nucleation of TCR-induced signaling complex for NFkappaB activation pathway is another crucial role of PDK1. Subcellular localization of PDK1 depends on conditions and has not been completely understood.
Product Type:
Antibodies Primary
Antibody Type:
polyclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Immunogen:
Peptide corresponding to the amino acids CIQEVWRQRYQSHPDAAVQ of human PDK1
Applications:
WB
Additional Info:
The rabbit polyclonal antibody recognizes PDK1, a serine/threonine proteinkinase of 63 kDa, which is a central activator of multiple signaling pathways coupled to a large number of growth-promoting stimuli.
NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.
Product Type:
Antibodies Primary
Antibody Type:
polyclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant mouse NTAL (aa 30-203).
Applications:
WB,ICC
Additional Info:
The polyclonal antibody reacts with intracellular part of Non-T cell Activation Linker (NTAL), also known as LAB (linker of activated B cells), a 25 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.
NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.
Product Type:
Antibodies Primary
Antibody Type:
polyclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant cytoplasmic domain (aa 91-243) of human NTAL.
Applications:
WB,IHC
Additional Info:
The polyclonal antibody recognizes a defined intracellular region (aa 91-243) of human Non-T cell Activation Linker (NTAL), also known as LAB (linker of activated B cells), a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.
Clone number:
PAb (485)
Application Details:
Western blotting: Recommended dilution: 1 ?g/ml; positive control: RAMOS human lymphoma cell line, THP-1 human monocytic leukemia cell line; negative control: JURKAT human T cell leukemia cell line; non-reducing conditions, 15% separating SDS-PAGE gel. Immunohistochemistry (paraffin sections): Recommended dilution:10 ?g/ml; positive tissue: spleen.
NK1.1 / CD161bc (also known as NKRP1, Ly55c, or Ly59) is a cell surface antigen expressed on NK cells and NK-T cells of certain mouse strains, which is being used for identification or antibody-mediated depletion of these cells in the respective strains. This antigen participates in NK cell activation, including production of interferon gamma, and release of cytotoxic granules.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
NK1-positive murine splenic and bone marrow cells
Applications:
FC,IP,IHC,FA
Additional Info:
The mouse monoclonal antibody PK136 recognizes NK1.1 antigen (NK cell marker) expressed by some mouse strains (e.g. C57BL/6, NZB, CE, C58, Ma/My), whereas other strains (e.g. BALB/c, AKR, CBA, C3H) do not express this antigen. This antibody detects a conformational epitope on Nkrp1c and Nkrp1b gene products.
NK1.1 / CD161bc (also known as NKRP1, Ly55c, or Ly59) is a cell surface antigen expressed on NK cells and NK-T cells of certain mouse strains, which is being used for identification or antibody-mediated depletion of these cells in the respective strains. This antigen participates in NK cell activation, including production of interferon gamma, and release of cytotoxic granules.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
NK1-positive murine splenic and bone marrow cells
Applications:
FC,IP,IHC,FA
Additional Info:
The mouse monoclonal antibody PK136 recognizes NK1.1 antigen (NK cell marker) expressed by some mouse strains (e.g. C57BL/6, NZB, CE, C58, Ma/My), whereas other strains (e.g. BALB/c, AKR, CBA, C3H) do not express this antigen. This antibody detects a conformational epitope on Nkrp1c and Nkrp1b gene products.
NK1.1 / CD161bc (also known as NKRP1, Ly55c, or Ly59) is a cell surface antigen expressed on NK cells and NK-T cells of certain mouse strains, which is being used for identification or antibody-mediated depletion of these cells in the respective strains. This antigen participates in NK cell activation, including production of interferon gamma, and release of cytotoxic granules.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
NK1-positive murine splenic and bone marrow cells
Applications:
FC,IP,IHC,FA
Additional Info:
The mouse monoclonal antibody PK136 recognizes NK1.1 antigen (NK cell marker) expressed by some mouse strains (e.g. C57BL/6, NZB, CE, C58, Ma/My), whereas other strains (e.g. BALB/c, AKR, CBA, C3H) do not express this antigen. This antibody detects a conformational epitope on Nkrp1c and Nkrp1b gene products.
MRCK alpha (myotonic dystrophy kinase-related Cdc42-binding kinase alpha) is a member of the dystrophia myotonica protein kinase (DMPK) family that functions downstream of Cdc42 in actin cytoskeletal reorganization. It is a serine/threonine kinase with multiple functional domains, including phorbol ester-responsive C1 domain. Three independent coiled-coil domains and the N-terminal region preceding the kinase domain are responsible for intermolecular interactions leading to MRCK alpha multimerization. The transautophosphorylation process is critical for regulation of MRCK alpha catalytic activities. Binding of phorbol esters to MRCK releases its autoinhibition, allowing N-terminal dimerization and subsequent kinase activation.
Product Type:
Antibodies Primary
Antibody Type:
polyclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Immunogen:
a peptide of RDFDGEDSDSPRHST sequence
Applications:
WB,ICC
Additional Info:
The polyclonal antibody anti-MRCK alpha recognizes myotonic dystrophy kinase-related Cdc42-binding kinase alpha (intracellular), a 180 kDa Ser/Thr kinase, which is responsible for reorganization of actin cytoskeleton.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PFR-03, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgM molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC,IP,IHC,ICC,WB,ELISA
Additional Info:
This mouse IgM monoclonal antibody (clone PFR-03) reacts with undefined epitope on a plant pathogen.
Clone number:
PFR-03
Antibody Isotype:
IgM
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Clone number:
PFR-03
Antibody Isotype:
IgM
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PFR-03, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgM molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Applications:
FC
Additional Info:
This mouse IgM monoclonal antibody (clone PFR-03) reacts with undefined epitope on a plant pathogen.
Clone number:
PFR-03
Antibody Isotype:
IgM
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PFR-03, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgM molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Additional Info:
This mouse IgM monoclonal antibody (clone PFR-03) reacts with undefined epitope on a plant pathogen.
Clone number:
PFR-03
Antibody Isotype:
IgM
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Applications:
FC,IHC,ICC,WB
Additional Info:
This mouse IgM monoclonal antibody (clone PFR-03) reacts with undefined epitope on a plant pathogen.
Clone number:
PFR-03
Antibody Isotype:
IgM
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Applications:
FC,IHC,ICC,WB
Additional Info:
This mouse IgM monoclonal antibody (clone PFR-03) reacts with undefined epitope on a plant pathogen.
Clone number:
PFR-03
Antibody Isotype:
IgM
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-07, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG3 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC,IP,IHC,ICC,WB,ELISA
Additional Info:
This mouse IgG3 monoclonal antibody (clone PPV-07) reacts with undefined epitope on a plant pathogen.
Clone number:
PPV-07
Antibody Isotype:
IgG3
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Clone number:
PPV-07
Antibody Isotype:
IgG3
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC,IHC,ICC,WB
Additional Info:
This mouse IgG3 monoclonal antibody (clone PPV-07) reacts with undefined epitope on a plant pathogen.
Clone number:
PPV-07
Antibody Isotype:
IgG3
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC,IHC,ICC,WB
Additional Info:
This mouse IgG3 monoclonal antibody (clone PPV-07) reacts with undefined epitope on a plant pathogen.
Clone number:
PPV-07
Antibody Isotype:
IgG3
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PLRV219, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC,IP,IHC,ICC,WB,ELISA
Additional Info:
This mouse IgG2b monoclonal antibody (clone PLRV219) reacts with undefined epitope on a plant pathogen.
Clone number:
PLRV219
Antibody Isotype:
IgG2b
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
KLH-coupled trinitrophenol
Applications:
ELISA,FC,IP,WB,IHC,ICC
Additional Info:
This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
Clone number:
MPC-11
Antibody Isotype:
IgG2b k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
KLH-coupled trinitrophenol
Applications:
FC
Additional Info:
This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
Clone number:
MPC-11
Antibody Isotype:
IgG2b k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
KLH-coupled trinitrophenol
Applications:
FC
Additional Info:
This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
Clone number:
MPC-11
Antibody Isotype:
IgG2b k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
KLH-coupled trinitrophenol
Applications:
FC
Additional Info:
This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
Clone number:
MPC-11
Antibody Isotype:
IgG2b k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
KLH-coupled trinitrophenol
Applications:
FC,IHC,ICC,WB
Additional Info:
This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
Clone number:
MPC-11
Antibody Isotype:
IgG2b k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
KLH-coupled trinitrophenol
Applications:
FC,IHC,ICC,WB
Additional Info:
This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
Clone number:
MPC-11
Antibody Isotype:
IgG2b k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
KLH-coupled trinitrophenol
Applications:
WB,ELISA,ICC,IHC,IP,FC
Additional Info:
This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
Clone number:
MPC-11
Antibody Isotype:
IgG2b k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
KLH-coupled trinitrophenol
Applications:
FC
Additional Info:
This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
Clone number:
MPC-11
Antibody Isotype:
IgG2b k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Applications:
FC,IP,WB,ICC,IHC,ELISA,FA
Additional Info:
This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Clone number:
MOPC-173
Antibody Isotype:
IgG2a k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-04, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC,IP,IHC,ICC,WB,ELISA,FA
Additional Info:
This mouse IgG2a monoclonal antibody (clone PPV-04) reacts with undefined epitope on a plant pathogen.
Clone number:
PPV-04
Antibody Isotype:
IgG2a
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-04, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC,IP,IHC,ICC,WB,ELISA
Additional Info:
This mouse IgG2a monoclonal antibody (clone PPV-04) reacts with undefined epitope on a plant pathogen.
Clone number:
PPV-04
Antibody Isotype:
IgG2a
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Applications:
FC,ICC,WB,IP,IHC,ELISA
Additional Info:
This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Clone number:
MOPC-173
Antibody Isotype:
IgG2a k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Applications:
FC
Additional Info:
This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Clone number:
MOPC-173
Antibody Isotype:
IgG2a k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Applications:
FC
Additional Info:
This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Clone number:
MOPC-173
Antibody Isotype:
IgG2a k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Applications:
FC
Additional Info:
This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Clone number:
MOPC-173
Antibody Isotype:
IgG2a k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Applications:
FC,IHC,ICC,WB
Additional Info:
This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Clone number:
MOPC-173
Antibody Isotype:
IgG2a k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Applications:
FC,IHC,ICC,WB
Additional Info:
This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Clone number:
MOPC-173
Antibody Isotype:
IgG2a k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Applications:
FC,IP,IHC,ICC,WB,ELISA
Additional Info:
This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Clone number:
MOPC-173
Antibody Isotype:
IgG2a k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-173, generated against an undefined antigen, does not react specifically with mouse, rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG2a molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
The transplantable plasmacytoma MOPC-173 was induced by intraperitoneal injection of mineral oils into BALB/c mice.
Applications:
FC
Additional Info:
This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Clone number:
MOPC-173
Antibody Isotype:
IgG2a k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC,IP,WB,IHC,ICC,ELISA,FA
Additional Info:
This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.
Clone number:
MOPC-21
Antibody Isotype:
IgG1 k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC,IP,WB,IHC,ICC,ELISA
Additional Info:
This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.
Clone number:
MOPC-21
Antibody Isotype:
IgG1 k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-06, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC,IP,IHC,ICC,WB,ELISA
Additional Info:
This mouse IgG1 monoclonal antibody (clone PPV-06) reacts with undefined epitope on a plant pathogen.
Clone number:
PPV-06
Antibody Isotype:
IgG1
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Additional Info:
This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.
Clone number:
MOPC-21
Antibody Isotype:
IgG1 k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Additional Info:
This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.
Clone number:
MOPC-21
Antibody Isotype:
IgG1 k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Additional Info:
This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.
Clone number:
MOPC-21
Antibody Isotype:
IgG1 k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Additional Info:
This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.
Clone number:
MOPC-21
Antibody Isotype:
IgG1 k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC,IHC,ICC,WB
Additional Info:
This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.
Clone number:
MOPC-21
Antibody Isotype:
IgG1 k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC,IHC,ICC,WB
Additional Info:
This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.
Clone number:
MOPC-21
Antibody Isotype:
IgG1 k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
WB,ELISA,IHC,ICC,IP,FC
Additional Info:
This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.
Clone number:
MOPC-21
Antibody Isotype:
IgG1 k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 ?g/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
Product Type:
Antibodies Isotype Control
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Additional Info:
This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.
Clone number:
MOPC-21
Antibody Isotype:
IgG1 k
Application Details:
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 ?g/ml of incubation mixture), change the conditions of your experiment to reduce the background.
The interferon gamma (IFN-gamma; 16-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12; its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN. IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant human interferon gamma
Applications:
IP,ELISA,RIA,FA
Additional Info:
The mouse monoclonal antibody NIB42 recognizes IFN-gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. Binds both glycosylated and non-glycosylated protein.
Clone number:
NIB42
Antibody Isotype:
IgG1
Application Details:
Functional application: Neutralization. ELISA: Capture antibody in combination with detection antibody 4S.B3.
The antibody 4H84 recognizes HLA-G molecule (39 kDa). HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.<br> <i>This product is for research and in vitro experimental use only. It is not to be used for any other commercial purpose. Use of this product to produce products for sale or for therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the Univessity of California.</i>
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
amino acids 61-83 of HLA-G of human origin
Applications:
WB,IP,IHC,ICC,ELISA
Additional Info:
The antibody 4H84 recognizes HLA-G molecule (39 kDa). HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.<br>_x000D_ <i>This product is for research and in vitro experimental use only. It is not to be used for any other commercial purpose. Use of this product to produce products for sale or for therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the Univesity of California.</i>_x000D_ _x000D_
The antibody 4H84 recognizes HLA-G molecule (39 kDa). HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.<br> <i>This product is for research and in vitro experimental use only. It is not to be used for any other commercial purpose. Use of this product to produce products for sale or for therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the Univessity of California.</i>
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
amino acids 61-83 of HLA-G of human origin
Applications:
WB,IP,IHC,ICC,ELISA
Additional Info:
The antibody 4H84 recognizes HLA-G molecule (39 kDa). HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.<br>_x000D_ <i>This product is for research and in vitro experimental use only. It is not to be used for any other commercial purpose. Use of this product to produce products for sale or for therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the Univesity of California.</i>_x000D_ _x000D_
The HIV protease (PR) hydrolyzes polyproteins of HIV virus into functional protein products that are essential for its assembly and subsequent activity. This maturation process occurs as the virion buds from the host cell. HIV protease inhibitors are used in the treatment of patients with AIDS and were considered the first breakthrough in over a decade of AIDS research. HIV protease inhibitors can lower the viral load carried by AIDS patents. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Bacterially expressed full-length HIV-1 protease
Applications:
WB,ELISA,FA
Additional Info:
The antibody 1696 recognizes free N-terminus of mature HIV protease (HIV-1 and HIV-2), an enzyme that hydrolyzes polyproteins of HIV viruses into functional proteins. <br>The antibody 1696 does not react with the precursor.
GABA B receptor is a G-protein-coupled inhibitory receptor of gamma-aminobutyric acid (GABA), and has important functions in brain by inhibition of adenylyl cyclase and modulation of G-protein-gated Ca2+ and K+ channels. GABA B receptor is comprised of two subunits, GB1 and GB2 with N-terminal extracellular and C-terminal intracellular domains. The GB1 subunit plays a critical role in ligand binding, whereas the GB2 subunit contains the determinants required for G-protein signaling. Multiple allosteric interactions between the two subunits are required for correct functioning of the receptor. There are two N-terminal splice variants of GB1 subunit, termed GB1a and GB1b; their expression in the central nervous system changes during the ontogenesis and differs between various regions of the brain.
Product Type:
Antibodies Primary
Antibody Type:
polyclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Immunogen:
Synthetic peptide (coupled with THG) derived from amino acid sequence 30-47 of mouse GABA B receptor 1b. Only 1 amino acid is different from immunogen derived from human GB1b.
Applications:
WB,ICC
Additional Info:
The polyclonal antibody recognizes N-terminus of mouse gamma-aminobutyric acid (GABA) B receptor 1b. GB1b apparent MW ~100 kDa._x000D_ _x000D_
CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Immunogen:
HUT-78 human T cell lymphoma cells
Applications:
FC,FA
Additional Info:
The antibody UT-1 reacts with an extracellular epitope on CD95 (Fas/APO-1), a 46 kDa glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. The antibody UT-1 does excellently induce Fas mediated apoptosis, similarly as CH11 antibody.
CD79b (Ig beta, B29) forms disulfide-linked heterodimer with CD79a (Ig alpha, MB1). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Purified CD79a/b (alpha/beta) dimers from WEHI231 cells_x000D_
Applications:
FC,IP,WB,ICC
Additional Info:
The Armenian hamster monoclonal antibody HM79 recognizes an extracellular epitope of mouse CD79b (CD79 beta, Ig beta), a component of B cell receptor (BCR) complex._x000D_
CD79b (Ig beta, B29) forms disulfide-linked heterodimer with CD79a (Ig alpha, MB1). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Purified CD79a/b (alpha/beta) dimers from WEHI231 cells_x000D_
Applications:
FC,IP,WB,ICC
Additional Info:
The Armenian hamster monoclonal antibody HM79 recognizes an extracellular epitope of mouse CD79b (CD79 beta, Ig beta), a component of B cell receptor (BCR) complex._x000D_
CD79b (Ig beta, B29) forms disulfide-linked heterodimer with CD79a (Ig alpha, MB1). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Purified CD79a/b (alpha/beta) dimers from WEHI231 cells_x000D_
Applications:
FC,IP,WB,ICC
Additional Info:
The Armenian hamster monoclonal antibody HM79 recognizes an extracellular epitope of mouse CD79b (CD79 beta, Ig beta), a component of B cell receptor (BCR) complex._x000D_
The CD66 molecules are 180-200 kDa glycoproteins of carcinoembryonic antigen family. They are present on all blood granulocytes and some tissue macrophages, but are absent from other hematopoietic cells. The expression of CD66 increases significantly on granulocytes upon their activation. CD66a (BGP1, CEACAM1) and CD66d (CGM1, CEACAM3), as well as CEACAM2 and 4, are transmembrane proteins, whereas CD66c (CEAL, NCA, CEACAM6) and CD66e (CEA, CEACAM5), as well as CEACAM7 and 8, are anchored to the plasma membrane by C-terminal glycosylphosphatidylinositol (GPI) lipid moiety.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Human granulocytes
Applications:
FC,IP,WB,IHC,ICC
Additional Info:
The mouse monoclonal antibody CLB-gran/10 (IH4Fc) detects human CD66acde antigen, a granulocyte marker, especially after cell stimulation. The antibody does not cross-react with normal human peripheral B cells, T cells, monocytes and platelets. Weak reactivity has been observed with malignant cells of pacients with B cell-derived CLL. In immunohistochemistry the antibody reacts with some tissue macrophages and carcinoma-expressed CEA. <br>_x000D_ <b>HLDA IV; WS Code M38</b>
CD54 (ICAM-1) is a member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Mouse NS-1 cells_x000D_
Applications:
FC,IP,WB,IHC,ICC,FA
Additional Info:
The rat monoclonal antibody YN1/1.7.4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation. _x000D_
CD54 (ICAM-1) is a member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Mouse NS-1 cells_x000D_
Applications:
FC,IP,WB,IHC,ICC,FA
Additional Info:
The rat monoclonal antibody YN1/1.7.4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation. _x000D_
CD54 (ICAM-1) is a member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Mouse NS-1 cells_x000D_
Applications:
FC,IP,WB,IHC,ICC,FA
Additional Info:
The rat monoclonal antibody YN1/1.7.4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation. _x000D_
CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells._x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1_x000D_
Applications:
FC,IP,WB,IHC,ICC
Additional Info:
The rat monoclonal antibody IM7 reacts with CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44._x000D_
CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells._x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1_x000D_
Applications:
FC,IP,WB,IHC,ICC
Additional Info:
The rat monoclonal antibody IM7 reacts with CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44._x000D_
CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells._x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1_x000D_
Applications:
FC,IP,WB,IHC,ICC
Additional Info:
The rat monoclonal antibody IM7 reacts with CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44._x000D_
CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells._x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1_x000D_
Applications:
FC,IP,WB,IHC,ICC
Additional Info:
The rat monoclonal antibody IM7 reacts with CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44._x000D_
CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells._x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1_x000D_
Applications:
FC,IP,WB,IHC,ICC
Additional Info:
The rat monoclonal antibody IM7 reacts with CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44._x000D_
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Immunogen:
Human thymocytes and T lymphocytes.
Applications:
FC,IP,FA
Additional Info:
The antibody MEM-115 recognizes an extracellular epitope in the D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (helper T cells) and also on monocytes, tissue macrophages and granulocytes. It is negative in Western blotting even with non-reduced samples of cell lysates.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Synthetic peptide corresponding to amino acids 151-164 of mouse CD3 zeta. _x000D_
Applications:
FC,IP,WB,ICC
Additional Info:
The Armenian hamster antibody H146-968 reacts with CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Synthetic peptide corresponding to amino acids 151-164 of mouse CD3 zeta. _x000D_
Applications:
FC,IP,WB,ICC
Additional Info:
The Armenian hamster antibody H146-968 reacts with CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Synthetic peptide corresponding to amino acids 151-164 of mouse CD3 zeta. _x000D_
Applications:
FC,IP,WB,ICC
Additional Info:
The Armenian hamster antibody H146-968 reacts with CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation._x000D_ The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Human thymocytes
Applications:
FC,WB,IHC,ICC
Additional Info:
The mouse monoclonal antibody SK7 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody reacts with the epsilon chain of the CD3 complex. The monoclonal antibodies SK7 and UCHT1 recognize overlapping epitopes._x000D_ <br><b>HLDA II; WS Code T118<br>_x000D_ HLDA III; WS Code T492</b>
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation._x000D_ The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Human thymocytes
Applications:
FC,WB,IHC,ICC
Additional Info:
The mouse monoclonal antibody SK7 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody reacts with the epsilon chain of the CD3 complex. The monoclonal antibodies SK7 and UCHT1 recognize overlapping epitopes._x000D_ <br><b>HLDA II; WS Code T118<br>_x000D_ HLDA III; WS Code T492</b>
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation._x000D_ The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Human thymocytes
Applications:
FC,WB,IHC,ICC
Additional Info:
The mouse monoclonal antibody SK7 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody reacts with the epsilon chain of the CD3 complex. The monoclonal antibodies SK7 and UCHT1 recognize overlapping epitopes._x000D_ <br><b>HLDA II; WS Code T118<br>_x000D_ HLDA III; WS Code T492</b>
CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells._x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Mouse T cell lymphoma EL-4 cells_x000D_
Applications:
FC,IP,IHC,ICC,FA
Additional Info:
The Syrian hamster monoclonal antibody 37.51 reacts with extracellular domain of mouse CD28 costimulatory receptor, a 45 kDa homodimeric protein expressed by thymocytes, mature T cells and NK cells._x000D_
TRAIL-R3 (CD263, TR3, DcR1, LIT, TRID), expressed mainly on neutrophils, belongs to receptors of TRAIL, a TNF-like membrane cytotoxic protein that induces apoptosis in many tumour cells, but not in normal cells. TRAIL-R3, however, is a GPI-anchored protein that lacks cytoplasmic death domain, thus it is unable to induce apoptosis and serves as a negative regulator of apoptotic signaling by competing for binding of TRAIL with death receptor 5 (DR5).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
TRAIL-R3 (aa 1-280) - hIgGhc fusion protein
Applications:
FC
Additional Info:
The antibody TRAIL-R3-02 reacts with TRAIL-R3, a 35 kDa GPI-anchored extracellular membrane protein expressed mainly on neutrophils.
TRAIL-R2 (CD262, DR5) is one of two TNF superfamily member intracellular death domain containing receptors for TRAIL (APO2L). Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including tumor necrosis factor (TNF) and Fas ligand in the TNF family through their death domain containing receptors, TNF receptor 1 (TNFR1) and Fas, respectively. Another member in the TNF family has been identified and designated TRAIL (for TNF related apoptosis inducing ligand) and Apo2L (for Apo2 ligand). Receptors for TRAIL include two death domain containing receptors, DR4 and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. DcR1 (also called TRID), like the related death receptors DR4 and DR5, contains two extracellular cysteine rich domains. However, DcR1 contains no intracellular death domain and is thus incapable of signaling apoptosis. It has been suggested DcR1 is responsible for TRAIL resistance in normal human tissues including heart, placenta, lung, liver, kidney, spleen, and bone marrow. DR5 is a member of the TNF receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor related apoptosis inducing ligand (TNFSF10/TRAIL/APO2L), and transduces apoptosis signal. Studies with FADD deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Recombinant fusion protein of human IgG heavy chain and extracellular domain of DR5.
Applications:
FC
Additional Info:
The antibody DR5-01-1 recognizes an extracellular domain of TRAIL-R2 (DR5). TRAIL-R2 is one of two TNF superfamily member intracellular death domain containing receptors for TRAIL (APO2L). _x000D_ _x000D_
CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
B6.1 CTL cell line
Applications:
FC,IP,IHC,FA
Additional Info:
The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4<sup>+</sup> T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
B6.1 CTL cell line
Applications:
FC,IP,IHC,FA
Additional Info:
The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4<sup>+</sup> T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
B6.1 CTL cell line
Applications:
FC,IP,IHC,FA
Additional Info:
The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4<sup>+</sup> T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
B6.1 CTL cell line
Applications:
FC,IP,IHC,FA
Additional Info:
The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4<sup>+</sup> T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
B6.1 CTL cell line
Applications:
FC,IP,IHC,FA
Additional Info:
The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4<sup>+</sup> T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
CD23 (Fc epsilon RII), the low affinity IgE receptor, is a 45 kDa type II membrane glycoprotein expressed more or less on eosinophils, follicular dendritic cells, Langerhans cells, mature B cells (mainly upon activation), EBV-transformed lymphoblasts, monocytes, and subpopulation of platelets. A soluble form of 37 kDa and other its fragments were also described. CD23 mediates IgE-dependent cytotoxicity by eosinophils and macrophages, and downregulates IgE secretion in response to high levels of IgE, involving release of pro-inflammatory cytokines.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
EBV-transformed human cells
Applications:
FC,IP
Additional Info:
The mouse monoclonal antibody EBVCS-5 recognizes an epitope located in the stalk region of human low affinity IgE receptor (CD23) between the 37 and 25 kDa cleavage sites.
CD23 (Fc epsilon RII), the low affinity IgE receptor, is a 45 kDa type II membrane glycoprotein expressed more or less on eosinophils, follicular dendritic cells, Langerhans cells, mature B cells (mainly upon activation), EBV-transformed lymphoblasts, monocytes, and subpopulation of platelets. A soluble form of 37 kDa and other its fragments were also described. CD23 mediates IgE-dependent cytotoxicity by eosinophils and macrophages, and downregulates IgE secretion in response to high levels of IgE, involving release of pro-inflammatory cytokines.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
EBV-transformed human cells
Applications:
FC,IP
Additional Info:
The mouse monoclonal antibody EBVCS-5 recognizes an epitope located in the stalk region of human low affinity IgE receptor (CD23) between the 37 and 25 kDa cleavage sites.
CD23 (Fc epsilon RII), the low affinity IgE receptor, is a 45 kDa type II membrane glycoprotein expressed more or less on eosinophils, follicular dendritic cells, Langerhans cells, mature B cells (mainly upon activation), EBV-transformed lymphoblasts, monocytes, and subpopulation of platelets. A soluble form of 37 kDa and other its fragments were also described. CD23 mediates IgE-dependent cytotoxicity by eosinophils and macrophages, and downregulates IgE secretion in response to high levels of IgE, involving release of pro-inflammatory cytokines.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
EBV-transformed human cells
Applications:
FC,IP
Additional Info:
The mouse monoclonal antibody EBVCS-5 recognizes an epitope located in the stalk region of human low affinity IgE receptor (CD23) between the 37 and 25 kDa cleavage sites.
CD23 (Fc epsilon RII), the low affinity IgE receptor, is a 45 kDa type II membrane glycoprotein expressed more or less on eosinophils, follicular dendritic cells, Langerhans cells, mature B cells (mainly upon activation), EBV-transformed lymphoblasts, monocytes, and subpopulation of platelets. A soluble form of 37 kDa and other its fragments were also described. CD23 mediates IgE-dependent cytotoxicity by eosinophils and macrophages, and downregulates IgE secretion in response to high levels of IgE, involving release of pro-inflammatory cytokines.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
EBV-transformed human cells
Applications:
FC,IP
Additional Info:
The mouse monoclonal antibody EBVCS-5 recognizes an epitope located in the stalk region of human low affinity IgE receptor (CD23) between the 37 and 25 kDa cleavage sites.
CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Whole hairy cell leukemia cells and membrane preparation
Applications:
FC
Additional Info:
The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes._x000D_ <br><b>HLDA IV; WS Code B48</b>
CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Whole hairy cell leukemia cells and membrane preparation
Applications:
FC
Additional Info:
The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes._x000D_ <br><b>HLDA IV; WS Code B48</b>
CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Whole hairy cell leukemia cells and membrane preparation
Applications:
FC
Additional Info:
The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes._x000D_ <br><b>HLDA IV; WS Code B48</b>
CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
RAMOS human lymphoma cell line
Applications:
FC,IHC
Additional Info:
The antibody MEM-269 reacts with CD20 (Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.
CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Human thymocytes
Applications:
FC,IP
Additional Info:
The mouse monoclonal antibody SK9 recognizes CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas.
CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Human thymocytes
Applications:
FC,IP
Additional Info:
The mouse monoclonal antibody SK9 recognizes CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas.
CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Human thymocytes
Applications:
FC,IP
Additional Info:
The mouse monoclonal antibody SK9 recognizes CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas.
CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Human thymocytes
Applications:
FC,IP
Additional Info:
The mouse monoclonal antibody SK9 recognizes CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas.
CD173 (blood group antigen H2) is a fucosylated saccharide (Fuc-alpha-1-2-Gal-beta-1-4-GlcNAc-beta) generated by beta-D-galactoside 2-alpha-L-fucosyltransferase (FUT1). CD173 belongs to markers of early hematopoiesis; it is expressed mainly on CD34-positive hematopoietic progenitor cells. CD173 is a precursor structure of CD174 (Lewis Y) and is also structurally related to CD15 (Lewis X). On endothelial cells CD173 and CD174 are concentrated on pseudopodial extensions responsible for initial contacts between endothelial cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Immunogen:
Human thrombocytes
Applications:
FC
Additional Info:
The antibody MEM-198 reacts with CD173 (H2), an extracellular saccharide antigen expressed mainly during early hematopoiesis; it is also expressed on endothelial cells.
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
A mixture of U266 and XG-1 human myeloma cell lines
Applications:
FC,IP,WB,IHC
Additional Info:
The mouse monoclonal antibody MI15 recognizes CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes.
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
A mixture of U266 and XG-1 human myeloma cell lines
Applications:
FC,IP,WB,IHC
Additional Info:
The mouse monoclonal antibody MI15 recognizes CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes.
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Immunogen:
U266 human peripheral blood myeloma cell line
Applications:
FC,IHC
Additional Info:
The antibody B-A38 recognizes CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes. _x000D_
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
U266 human peripheral blood myeloma cell line
Applications:
FC,IHC
Additional Info:
The antibody B-A38 recognizes CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes. _x000D_
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
A mixture of U266 and XG-1 human myeloma cell lines
Applications:
FC,IP,WB,IHC
Additional Info:
The mouse monoclonal antibody MI15 recognizes CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes.
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
U266 human peripheral blood myeloma cell line
Applications:
FC,IHC
Additional Info:
The antibody B-A38 recognizes CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes. _x000D_
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
A mixture of U266 and XG-1 human myeloma cell lines
Applications:
FC,IP,WB,IHC
Additional Info:
The mouse monoclonal antibody MI15 recognizes CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes.
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
A mixture of U266 and XG-1 human myeloma cell lines
Applications:
FC,IP,WB,IHC
Additional Info:
The mouse monoclonal antibody MI15 recognizes CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes.
CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Immunogen:
U937 human Caucasian hystiocytic lymphoma cell line
Applications:
FC,IHC,ICC
Additional Info:
The antibody B-T47 recognizes CD107a, a 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane. _x000D_
The antibody HEB-29 reacts with human blood group B. The specifity of the antibody HEB-29 was confirmed by comparison of specifity and reactivity to standard reagent using >5.000 samples of blood.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mixture of erythrocytes of group B and glycoprotein fraction isolated from saliva of secretors with blood group B.
Applications:
AGG,IHC
Additional Info:
The antibody HEB-29 reacts with human blood group B. The specifity of the antibody HEB-29 was confirmed by comparison of specifity and reactivity to standard reagent using >5 000 samples of blood.
The antibody HE-10 agglutinates erythrocytes of group A but does not agglutinate erythrocytes of group B and 0. Study with specific oligosaccharides showed that the antibody HE-10 reacts with A and H antigens with chain types 3 and 4 and it does not react with A disaccharide, A trisaccharide, A type 1, A type 2, ALe<sup>b</sup>.<br>The antibody HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.
Applications:
IHC
Additional Info:
The mouse monoclonal antibody HE-10 agglutinates erythrocytes of group A, and is excellent as a tumour marker in patients of blood group B and 0. It does not agglutinate erythrocytes of group B and 0. Study with specific oligosaccharides showed that the antibody HE-10 reacts with A and H antigens with chain types 3 and 4 and it does not react with A disaccharide, A trisaccharide, A type 1, A type 2, ALeb. The antibody HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues.
Clone number:
HE-10
Antibody Isotype:
IgM
Application Details:
Immunohistochemistry (paraffin sections): The antibody HE-10 is excellent as a tumour marker in patients of blood group B and 0. Flow cytometry: Recommended dilution: 1-3 : 100.
The antibody HE-24 distinguishes blood group A<sub>1</sub>B from A<sub>2</sub>B. The specifity of the antibody HE-24 was verified on >1000 samples of blood.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Glycoprotein fraction isolated from saliva of secretors with blood group A.
Applications:
AGG
Additional Info:
The antibody HE-24 distinguishes blood group A1B from A2B. The specifity of the antibody HE-24 was verified on >1000 samples of blood.
Human blood group A antigen belongs to a group of carbohydrate determinats carried on both glycolipids and glycoproteins; it is detected on erythrocytes and certain epithelial cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.
Applications:
IHC,AGG
Additional Info:
The antibody HE-193 recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1 and 2, A antigens with chain types 3, 4, 5, 6) and Forssman antigen.
Human blood group A antigen belongs to a group of carbohydrate determinats carried on both glycolipids and glycoproteins; it is detected on erythrocytes and certain epithelial cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.
Applications:
AGG
Additional Info:
The antibody HE-195 recognizes human blood group A including weak variants A3, AX, A3B, AXB. The specifity of antibody HE-195 was confirmed by comparison of specifity and reactivity to standard reagent using >5.000 samples of blood.
Bim (Bcl2-interacting mediator) is a pro-apoptotic protein of BH3 domain-only subgroup of the Bcl2 family. It has important roles in initiation of apoptosis in response to many death stimuli. Bim is an important regulator of B and T cell negative selection and is also an essential regulator of T cell apoptosis during termination of an immune response. Bim is constitutively expressed in many cell types but it is maintained in an inactive form through binding to the microtubule-associated dynein motor complex._x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
The Armenian hamster antibody Ham151-149 reacts with mouse Bim, a 19-24 kDa pro-apoptotic protein (Bcl-2 family) which regulates immunological responses._x000D_
The ZAP70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP70 gene mutations resulting in lower ZAP70 protein expression levels or expression of catalytically inactive ZAP70 proteins, have been identified. ZAP70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency.In patients with chronic lymphocytic leukemia (B-CLL), ZAP70 expression on B cell was shown to be correlated with disease progression and survival. ZAP70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP70 on Tyr493 in the activation loop. ZAP70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.
Product Type:
Antibodies Primary
Antibody Type:
polyclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP70 with histidine tag
Applications:
WB
Additional Info:
The polyclonal antibody recognizes C-terminal part of human ZAP70 protein tyrosine kinase (intracellular antigen). ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
The ZAP70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP70 gene mutations resulting in lower ZAP70 protein expression levels or expression of catalytically inactive ZAP70 proteins, have been identified. ZAP70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency.In patients with chronic lymphocytic leukemia (B-CLL), ZAP70 expression on B cell was shown to be correlated with disease progression and survival. ZAP70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP70 on Tyr493 in the activation loop. ZAP70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP70 with histidine tag
Applications:
FC,WB,ICC
Additional Info:
The antibody ZAP-03 reacts with ZAP70, a 70 kDa protein tyrosine kinase expressed in T and NK cells (intracellular antigen). ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
Clone number:
ZAP-03
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: Intracellular staining; recommended dilution: 2-5 ?g/ml; positive control: HPB-ALL human peripheral blood T cell leukemia cell line. Western blotting: Recommended dilution: 0,5 ?g/ml; positive control: HPB-ALL human peripheral blood T cell leukemia cell line; negative control: RAMOS human Burkitt lymphoma cell line; reducing conditions, 10% separating gel.
Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
IP,WB,IHC,ICC
Additional Info:
The antibody VI-10 reacts with vimentin, a 57 kDa intermediate filament intracellular protein expressed in variety of mesenchymal and mesodermal cell types.
Clone number:
VI-10
Antibody Isotype:
IgM
Application Details:
Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line. Immunohistochemistry (paraffin sections): Recommended dilution: 5 ?g/ml, positive tissue: skin fibroblast.Western blotting: Recommended dilution: 1-2 ?g/ml.
Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Bacterially expressed full-length human vimentin
Applications:
IHC,FC,WB,ICC,ELISA
Additional Info:
The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament intracellular protein expressed on a wide variety of mesenchymal and mesodermal cell types.
Clone number:
VI-RE/1
Antibody Isotype:
IgG1
Application Details:
ELISA: The antibody VI-RE/1 recognizes different epitope on human vimentin than the antibody VI-01 (IgM). Flow cytometry: Recommended dilution: 1-4 ?g/ml. Intracellular staining.Western blotting: Recommended dilution: 1-2 ?g/ml.Immunocytochemistry: Recommended dilution: 5-10 ?g/ml.
Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Pellet of porcine brain cold stable proteins after depolymerization of microtubules.
Applications:
IHC,WB,ICC
Additional Info:
The antibody VI-01 reacts with vimentin, a 57 kDa intermediate filament intracellular protein expressed in variety of mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscle desmin.
Clone number:
VI-01
Antibody Isotype:
IgM
Application Details:
Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line.
Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Bacterially expressed full-length human vimentin
Applications:
FC
Additional Info:
The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament intracellular protein expressed on a wide variety of mesenchymal and mesodermal cell types.
Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Bacterially expressed full-length human vimentin
Applications:
FC
Additional Info:
The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament intracellular protein expressed on a wide variety of mesenchymal and mesodermal cell types.
VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Applications:
FC,WB,ICC
Additional Info:
The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.
Clone number:
Hs-14
Antibody Isotype:
IgM
Application Details:
Immunocytochemistry: Recommended dilution: 10 ?g/ml, membrane permeabilization (acetone) is essential. The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought). Flow cytometry: Intraacrosomal staining; recommended dilution: 3-12 µg/ml.
VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Applications:
FC
Additional Info:
The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.
Clone number:
Hs-14
Antibody Isotype:
IgM
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 50 tests. Intraacrosomal staining.
Ubinuclein 1 (UBN1) is a ubiquitously expressed evolutionarily conserved protein which binds to proliferation-promoting genes that are repressed by formation of senescence-associated heterochromatin foci (SAHF). Ubinuclein 1 associates with various transcription factors and with histone methyltransferase activity, is indispensable for SAHF formation and appears to be a regulator of senescence. Although in most cells ubinuclein is localized to the nucleus, in cells forming tight junctions it is recruited to the cell adhesion complexes, dependently on the cell density.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant protein corresponding to amino acids 1-190 of ubinuclein 1.
Applications:
IP,WB
Additional Info:
The antibody UBN1-02 recognizes N-terminal part of ubinuclein 1 (UBN1), a widely expressed nuclear and adhesion complex protein. Western blotting analysis reveals UBN1 as a 165 kDa band.
Clone number:
UBN1-02
Antibody Isotype:
IgG
Application Details:
Western blotting: Recommended dilution: 1-5 ?g/ml; positive control: HeLa cell line.
TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant intracellular domain (aa 29-186) of human TRIM.
Applications:
FC,IP,WB
Additional Info:
The antibody TRIM-04 recognizes an intracellular epitope of T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.
Clone number:
TRIM-04
Antibody Isotype:
IgG2a
Application Details:
Flow cytometry: Intracellular staining. Western blotting: Non-reducing conditions.
TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Recombinant intracellular domain (aa 29-186) of human TRIM.
Applications:
FC
Additional Info:
The antibody TRIM-04 recognizes an intracellular epitope of T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.
Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Purified porcine transferrin.
Applications:
WB,IHC,ICC,ELISA,RIA,FA
Additional Info:
The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
Clone number:
HTF-14
Antibody Isotype:
IgG1
Application Details:
Functional application: The antibody HTF-14 blocks binding of transferrin to its receptor. Immunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml; positive tissue: placenta. Western blotting: non-reducing conditions, recommended dilution: 1-2 ?g/ml.
Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Purified porcine transferrin.
Applications:
WB,IHC,ICC,ELISA,RIA,FA
Additional Info:
The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
Clone number:
HTF-14
Antibody Isotype:
IgG1
Application Details:
Functional application: The antibody HTF-14 blocks binding of transferrin to its receptor. Immunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml; positive tissue: placenta. Western blotting: non-reducing conditions, recommended dilution: 1-2 ?g/ml.
Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Purified porcine transferrin.
Applications:
WB,IHC,ICC,ELISA,RIA
Additional Info:
The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
TPX2 is a microtubule-associated protein, which is a critical regulator of mitosis. At the beginning of mitosis, TPX2 is released and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. After completion of mitosis the TPX2 protein disappears, but has also role in DNA damage response. Its overexpression has been demonstrated in many types of carcinomas. TPX2 belongs to the markers of worse tumor prognosis. On the other hand, down-regulation of TPX2 can inhibit cancer cell proliferation, migration and invasion.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Human TPX2
Applications:
IP,WB,IHC,ICC,ELISA
Additional Info:
The mouse monoclonal antibody TPX2-01 recognizes the epitope EPFVPKKEKKS (aa 636-646 in human) of TPX2, a microtubule-associated intracellular critical regulator of mitosis, which is overexpressed in many types of tumors and is a marker of worse prognosis in various cancers.
TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant human TNF-alpha
Applications:
WB,ELISA,FA
Additional Info:
The mouse monoclonal antibody MAb1 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
Clone number:
MAb1
Antibody Isotype:
IgG1 k
Application Details:
Functional application: Neutralization. ELISA: It can be used as capture antibody in combination with biotinylated antibody MAb11.
TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant human TNF-alpha
Applications:
FC,IHC,ICC,ELISA
Additional Info:
The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
Clone number:
MAb11
Antibody Isotype:
IgG1 k
Application Details:
ELISA: Biotinylated MAb11 can be used as a detection antibody in combination with capture antibody MAb1. Immunohistochemistry (frozen sections): paraformaldehyde-fixed, saponin-treated frozen tissue sections. Flow cytometry: Intracellular staining.
TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant human TNF-alpha
Applications:
WB,ELISA
Additional Info:
The mouse monoclonal antibody MAb1 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
Clone number:
MAb1
Antibody Isotype:
IgG1 k
Application Details:
ELISA: It can be used as capture antibody in combination with biotinylated antibody MAb11.
TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Recombinant human TNF-alpha
Applications:
FC
Additional Info:
The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
Clone number:
MAb11
Antibody Isotype:
IgG1 k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Recombinant human TNF-alpha
Applications:
FC
Additional Info:
The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
Clone number:
MAb11
Antibody Isotype:
IgG1 k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.
TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Recombinant human TNF-alpha
Applications:
FC
Additional Info:
The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
Clone number:
MAb11
Antibody Isotype:
IgG1 k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
Tissue non-specific alkaline phosphatase (TNAP), also known as liver/bone/kidney alkaline phosphatase, or MSCA-1 (mesenchymal stem cell antigen 1) is a selective marker for the prospective isolation of bone marrow-derived mesenchymal stem cells and mesenchymal stem-like cells. It is expressed at high levels in liver, bone, kidney, or endometrium, as well as on embryonic stem cells (ESCs). TNAP also plays a role in bone mineralization. Mutations in TNAP gene are associated with hypercalcemia and skeletal defects (hypophosphatasia).
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
WERI-RB-1 retinoblastoma cell line
Applications:
FC
Additional Info:
The mouse monoclonal antibody W8B2B10 recognizes TNAP (tissue non-specific alkaline phosphatase), an ectoenzyme expressed mainly on embryonic stem cells, liver, bone, and kidney cells. This antibody is suitable for characterization of bone marrow-derived MSCs, iPSCs, and ESCs.
Tissue non-specific alkaline phosphatase (TNAP), also known as liver/bone/kidney alkaline phosphatase, or MSCA-1 (mesenchymal stem cell antigen 1) is a selective marker for the prospective isolation of bone marrow-derived mesenchymal stem cells and mesenchymal stem-like cells. It is expressed at high levels in liver, bone, kidney, or endometrium, as well as on embryonic stem cells (ESCs). TNAP also plays a role in bone mineralization. Mutations in TNAP gene are associated with hypercalcemia and skeletal defects (hypophosphatasia).
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
WERI-RB-1 retinoblastoma cell line
Applications:
FC
Additional Info:
The mouse monoclonal antibody W8B2B10 recognizes TNAP (tissue non-specific alkaline phosphatase), an ectoenzyme expressed mainly on embryonic stem cells, liver, bone, and kidney cells. This antibody is suitable for characterization of bone marrow-derived MSCs, iPSCs, and ESCs.
Tissue non-specific alkaline phosphatase (TNAP), also known as liver/bone/kidney alkaline phosphatase, or MSCA-1 (mesenchymal stem cell antigen 1) is a selective marker for the prospective isolation of bone marrow-derived mesenchymal stem cells and mesenchymal stem-like cells. It is expressed at high levels in liver, bone, kidney, or endometrium, as well as on embryonic stem cells (ESCs). TNAP also plays a role in bone mineralization. Mutations in TNAP gene are associated with hypercalcemia and skeletal defects (hypophosphatasia).
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
WERI-RB-1 retinoblastoma cell line
Applications:
ICC,FC
Additional Info:
The mouse monoclonal antibody W8B2B10 recognizes TNAP (tissue non-specific alkaline phosphatase), an ectoenzyme expressed mainly on embryonic stem cells, liver, bone, and kidney cells. This antibody is suitable for characterization of bone marrow-derived MSCs, iPSCs, and ESCs.
TIAR is an ubiquitously expressed RNA-binding protein, which regulates translational control, splicing, and other activities, including apoptosis. TIAR attenuates CDK1 activity, and is essential for the G2/M checkpoint. It accumulates in nuclear foci in late G2 phase and prophase in cells under replication stress. In steady state TIAR shuttles between the cytoplasm and the nucleus, probably as a part of nucleocytoplasmic transport of mRNA, but under stress conditions it accumulates mRNA molecules in granules and prevents their translation. Nucleolytic activity of TIAR against attacked target cells of cytotoxic lymphocytes has also been reported. Similarly, e.g. in permeabilized thymocytes TIAR triggers DNA fragmentation.
Thyroid disorders are often associated with autoimmune diseases and thyroid cancer.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
thyroid follicular cells
Applications:
IHC
Additional Info:
The antibody 2H11 recognizes thyroglobulin (TG), a 610 kDa extracellular secreted glycoprotein specific to the thyroid gland. TG is mainly expressed on thyroid follicular cells (99,1 %).
TFG (TRK-fused gene protein) is a regulatory protein with not fully understood function. Its defects are associated with various carcinomas, such as e.g. melanoma, thyroid papillary carcinoma, or glioma. TFG structure (multiple protein interaction motifs) indicated it can be an adaptor protein. It has been demonstrated TFG interacts with proteins modulating the NFkappaB pathway (TANK and NEMO). TNG enhances the effect of TNFalpha, TANK, TRAF2 and TRAF6 in inducing NFkappaB activity.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Human recombinant protein Trx-his-NTFG
Applications:
IP,WB,ICC
Additional Info:
The mouse monoclonal antibody TFG-03 recognizes TFG, an approximately 50 kDa intracellular protein with regulatory functions.
Tenascin C is an approximately 250 kDa extracellular matrix glycoprotein with important roles in the nervous system, as it promotes correct migration of growing axons during development and during neuronal regeneration. It is also involved in synaptic plasticity. Ligands of tenascin C are integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3, and alpha-V/beta-6. Similarly to neural cells, it also stimulates angiogenesis by promoting elongation and migration of endothelial cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Protein preparation from a homogenate of a human mammary tumour specimen.
Applications:
IHC,IP,WB
Additional Info:
The antibody T2H5 recognizes tenascin, a large hexameric extracellular matrix glycoprotein.
Clone number:
T2H5
Antibody Isotype:
IgG1
Application Details:
Immunohistochemistry (paraffin sections): Immunohistochemical detection of tenascin is valuable for studies of tissue differentiation and tumour growth. The antibody T2H5 is excellent for staining of paraffin-embedded tissue sections.Western blotting: Recommended dilution: 1-2 ?g/ml.
Mycobacterium tuberculosis protein Tb7.7, also known as Rv2654c, is being used for lymfocyte stimulation against Mycobacterium in combination with EsaT-6 and CFP-10 proteins.
Product Type:
Antibodies Primary
Antibody Type:
polyclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant Tb7.7 protein (produced in E. coli)
Applications:
WB,ELISA
Additional Info:
The rabbit polyclonal antibody to Tb7.7 reacts with the Mycobacterium tuberculosis protein Tb7.7 (Rv2654c).
The Mycobacterium tuberculosis antigen Tb10.4, also known as EsxH, Rv0288, ESAT-6 like protein EsxH, or Cfp7, is a conserved bacterial protein which effectively induces immune response to M. tuberculosis infection. It is a promissing vaccination tool.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant Tb10.4 protein produced in E. coli
Applications:
WB,ELISA
Additional Info:
The rabbit polyclonal antibody to Tb10.4 reacts with Mycobacterium tuberculosis protein Tb10.4 (EsxH).
The Mycobacterium tuberculosis antigen Tb10.3, also known as EsxR, ESAT-6 like protein 9 (ES6_9), or Rv3019c, is an almost uncharacterized conserved bacterial protein.
Product Type:
Antibodies Primary
Antibody Type:
polyclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant Tb10.3 protein (produced in E. coli)
Applications:
WB,ELISA
Additional Info:
The rabbit polyclonal antibody to Tb10.3 reacts with Mycobacterium tuberculosis protein Tb10.3 (EsxR).
The p53 family of proteins includes three members, p53, p63, and p73. The protein p63 is encoded by TP63 gene, which gives rise to protein isoforms with different properties and functions due to the presence (TAp63) or absence (deltaNp63) of an N-terminal transactivation domain. Immunohistochemistry of p63 has a clinical relevance for certain tumor types, but investigations have been hampered by a lack of well characterized antibodies that are specific for p63, do not cross-react with the related p53 and p73 proteins, and allow for discrimination between p63 isoforms TAp63 and deltaNp63 with opposite functional properties.
Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant fragment (aa 5-360) of human Syk.
Applications:
IP,WB,IHC,ICC
Additional Info:
The antibody SYK-01 reacts with protein tyrosine kinase p72Syk (Syk; an intracellular antigen), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FcepsilonRI).
Clone number:
SYK-01
Antibody Isotype:
IgG1
Application Details:
Western blotting: Recommended dilution: 1-2 ?g/ml; positive control: RBL rat basophilic leukemia cell line, A-431 human epidermoid carcinoma cell line, RAMOS lymphoma cell line, U-937 human histiocytic lymphoma cell line, JURKAT human peripheral blood T cell leukemia cell line; negative control: HeLa human cervix carcinoma cell line; non-reducing conditions. Immunohistochemistry (paraffin sections): Recommended dilution: 5 ?g/ml; positive tissue: tonsil B cells.
STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Synthetized peptide (C-terminal cytoplasmic part of STIM1).
Applications:
IP,WB,IHC,ICC
Additional Info:
The mouse monoclonal antibody CDN3H4 reacts with a cytoplasmic epitope of human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.
Clone number:
CDN3H4
Antibody Isotype:
IgG1
Application Details:
Immunocytochemistry: Methanol-aceton fixation; positive control: HeLa human cervix carcinoma cell line.Immunohistochemistry (paraffin sections): Recommended dilution: 5 ?g/ml.Western blotting: Recommended dilution: 1 ?g/ml; positive control: RBL rat basophilic leukemia cell line; both reducing and non-reducing conditions.
STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
STAT1 peptide sequence 8-23 (QLDSKFLEQVHQLYD) conjugated to KLH.
Applications:
IP,WB
Additional Info:
The antibody SM2 recognizes an epitope included within amino acids 8-23 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.
STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
STAT1 peptide sequence 721-733 (DNLLPMSPEEFDE).
Applications:
IP,WB,IHC
Additional Info:
The antibody SM1 recognizes an epitope included within amino acids 721-733 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.
The guanine nucleotide exchange factor Sos (son-of-sevenless) is a complex multidomain protein that activates the small GTPase Ras (H-Ras, K-Ras, N-Ras, but not functionally distinct R-Ras) in response to receptor tyrosine kinase stimulation. Nucleotide exchange activity of Sos is stimulated by allosteric Ras binding. By another (separable) guanine exchange factor domain domain Sos modulates activity of Rac/Rho GTPases. Sos thus integrates signals that affect both gene expression and cytoskeletal reorganization; the Sos-mediated Ras-activation and Rac activation differ in composition and stability of the formed complex.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Peptide corresponding to amino acids THPSMHRDGPPLLENAHSS of human Sos protein.
Applications:
WB,ICC
Additional Info:
The antibody SOS-01 reacts with human Sos, an ubiquitously expressed 150 kDa intracellular protein.
Clone number:
SOS-01
Antibody Isotype:
IgG1
Application Details:
Western blotting: Recommended dilution: 1 ?g/ml; positive control: HeLa human cervix carcinoma cell line, reducing conditions.
SOCS3 (suppressor of cytokine signaling 3), also known as CIS3 (cytokine-inducible SH2 protein 3) is a negative regulator of particular cytokine signaling pathways. SOCS3 is induced by a variety of cytokines and other stimuli, such as erythropoietin, leptin and lipopolysaccharides and inhibits tyrosinkinase activity of JAK kinases, or e.g. JNK phosphorylation. SOCS3 modulates cytokine-mediated and neoplastic-proliferative responses and is involved also in maintaining leukocytes in quiescent state until antigen stimulation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Full-length SOCS3 protein.
Applications:
WB
Additional Info:
The antibody SO1 reacts with SOCS3, an intracellular cytokine signaling inhibitor.
Clone number:
SO1
Antibody Isotype:
IgG2b
Application Details:
Western blotting: Positive control: HeLa human cervix carcinoma cell line, reducing conditions.
SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor protein which translocates to the plasma mambrane and is involved in multiple signaling pathways in T cells, mast cells, neutrophils and platelets; B cells express its analog SLP65/BLNK (B cell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinases and couples them to the phosphorylation and activation of PLC-gamma. Via Gads or Grb2, SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. The SH2 domain of SLP76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.
Product Type:
Antibodies Primary
Antibody Type:
polyclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Bacterially expressed fusion protein representing amino acids 216-434 of human SLP76 with histidine tag
Applications:
WB,IHC
Additional Info:
The polyclonal antibody reacts with SLP76, a 76kDa cytosolic adaptor protein that is involved in signaling of various hematopoietic cells, such as T cells, mast cells or neutrophils; in B cells, however, it is replaced by SLP65.
Clone number:
PAb (412)
Application Details:
Western blotting: Positive control: JURKAT human T cell leukemia cell lysate, reducing conditions; Immunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml, positive tissue: thymus.
SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor protein which translocates to the plasma mambrane and is involved in multiple signaling pathways in T cells, mast cells, neutrophils and platelets; B cells express its analog SLP65/BLNK (B cell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinases and couples them to the phosphorylation and activation of PLC-gamma. Via Gads or Grb2, SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. The SH2 domain of SLP76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Bacterially expressed fusion protein representing amino acids 216-434 of human SLP76 with histidine tag
Applications:
WB,IHC
Additional Info:
The monoclonal antibody reacts with SLP76, a 76kDa cytosolic adaptor protein that is involved in signaling of various hematopoietic cells, such as T cells, mast cells or neutrophils; in B cells, however, it is replaced by SLP65.
SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Bacterially produced recombinant intracellular fragment of human SIT.
Applications:
FC,IP,WB
Additional Info:
The antibody SIT-01 reacts with an intracellular epitope of SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with murine SIT.
Clone number:
SIT-01
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: Intracellular staining. Western blotting: SIT migrates as an approximately 40 kDa protein that is reduced to approximately 20 kDa by endoglycosidase treatment.
SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Bacterially produced recombinant intracellular fragment of human SIT.
Applications:
FC
Additional Info:
The antibody SIT-01 reacts with an intracellular epitope of SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with murine SIT.
SHIP-1 (SH2 domain containing inositol phosphatase-1) is a 5´inositol phosphatase that regulates cell responses in lymphocytes and myeloid cells by hydrolyzing the second messenger PI(3,4,5) trisphosphate. SHIP-1 is recruited upon engagement of both inhibitory and activatory receptors, such as FcgammaRIIB, Fcgamma RIII, FcepsilonRI or cytokine and growth factor receptors, and supresses PI3K-dependent signaling, down-regulates cell migration and invasion of transformed cells and phagocytosis. SHIP-1 also serves as a scaffold for the recruitment of other proteins to the plasma membrane.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.
Applications:
FC,WB
Additional Info:
The antibody SHIP-01 reacts with SHIP-1, a phosphoinositide phosphatase largely confined to hematopoietic cells (intracellular antigen). Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 kDa.
Clone number:
SHIP-01
Antibody Isotype:
IgG2a
Application Details:
Flow cytometry: Intracellular staining; recommended dilution: 2-5 ?g/ml; positive control: human blood leukocytes. Western blotting: Positive control: RAMOS human cell line, reducing conditions.
SHIP-1 (SH2 domain containing inositol phosphatase-1) is a 5´inositol phosphatase that regulates cell responses in lymphocytes and myeloid cells by hydrolyzing the second messenger PI(3,4,5) trisphosphate. SHIP-1 is recruited upon engagement of both inhibitory and activatory receptors, such as FcgammaRIIB, Fcgamma RIII, FcepsilonRI or cytokine and growth factor receptors, and supresses PI3K-dependent signaling, down-regulates cell migration and invasion of transformed cells and phagocytosis. SHIP-1 also serves as a scaffold for the recruitment of other proteins to the plasma membrane.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.
Applications:
FC,WB
Additional Info:
The antibody SHIP-02 reacts with SHIP-1, a phosphoinositide phosphatase largely confined to hematopoietic cells. Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 kDa.
Clone number:
SHIP-02
Antibody Isotype:
IgG2a
Application Details:
Flow cytometry: Intracellular staining; recommended dilution: 2-5 ?g/ml; positive control: human blood leukocytes. Western blotting: Positive control: RAMOS human cell line, reducing conditions.
SCIMP (SLP adaptor and Csk interacting membrane protein), also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It is associated with tetraspanin-enriched microdomains (together with MHC II). There is a close relationship between SCIMP and tyrosinkinase Lyn, which is constitutively bound to it by its SH3 domain. After MHC II-mediated stimulation in the immunological synapse SCIMP becomes phosphorylated at several tyrosine residues and provides docking sites for Grb2 and SLP65 or SLP76 adaptors transducing the signal downstream, as well as for the kinase Csk with modulatory roles.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant intracellular part of human SCIMP
Applications:
FC,IP,WB,ICC
Additional Info:
The mouse monoclonal antibody NVL-07 recognizes intracellular part of human transmembrane adaptor SCIMP. This protein of 17 kDa predicted Mw migrates as a 22 kDa band on SDS PAGE.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
TCR alpha/beta-negative CD3-positive rat T cell hybridoma III.89.1.4 line
Applications:
FA,FC,IP,IHC
Additional Info:
The mouse monoclonal antibody V65 recognizes an extracellular epitope of TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
TCR alpha/beta-negative CD3-positive rat T cell hybridoma III.89.1.4 line
Applications:
FC,IP,IHC
Additional Info:
The mouse monoclonal antibody V65 recognizes an extracellular epitope of TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
TCR alpha/beta-negative CD3-positive rat T cell hybridoma III.89.1.4 line
Applications:
FC
Additional Info:
The mouse monoclonal antibody V65 recognizes an extracellular epitope of TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Rat T blasts and erythrocytes
Applications:
WB,IHC,ICC,FA,FC,IP
Additional Info:
The mouse monoclonal R73 recognizes an extracellular epitope TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Rat T blasts and erythrocytes
Applications:
FC,IP,WB,IHC,ICC
Additional Info:
The mouse monoclonal R73 recognizes an extracellular epitope TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Rat T blasts and erythrocytes
Applications:
FC
Additional Info:
The mouse monoclonal R73 recognizes an extracellular epitope TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Rat T blasts and erythrocytes
Applications:
FC
Additional Info:
The mouse monoclonal R73 recognizes an extracellular epitope TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.
PLSCR1 (phospholipid scramblase 1) is a multiply palmitoylated endofacial plasma membrane protein containing several SH3 and WW domain binding motives. In the plasma membrane, PLSCR1 plays a role in transbilayer lipid redistributions and signal transduction. Nonpalmitoylated PLSCR1, however, is able to be transported into the nucleus and bind DNA. PLSCR1 potentiates the antiviral activity of interferon and its expression is highly induced by interferons and growth factors.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Pooled lipid raft fraction isolated from RBL-2H3 cells
Applications:
IP,WB,ICC,FC
Additional Info:
The antibody 13A6 [TEC-23] binds to an intracellular epitope of rat Phospholipid Scramblase 1 (PLSCR1), an 37-49 kDa protein, accelerating bidirectional movement of plasma membrane phospholipids under conditions of elevated calcium.
Clone number:
13A6 [TEC-23]
Antibody Isotype:
IgG2a
Application Details:
Western blotting: Recommended dilution: 1 ?g/ml, positive control: RBL (rat basophilic leukemia) cell line; both reducing and non-reducing conditions.Flow cytometry: Intracellular staining.
The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
CD8 positive Wistar rat splenic T cell hybridomas
Applications:
FC,WB,FA,IP,IHC
Additional Info:
The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor (extracellular epitope), expressed on T cell subsets and some other cell types, such as macrophages.
The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
CD8 positive Wistar rat splenic T cell hybridomas
Applications:
FC,IP,WB,IHC
Additional Info:
The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor (extracellular epitope), expressed on T cell subsets and some other cell types, such as macrophages.
The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
CD8 positive Wistar rat splenic T cell hybridomas
Applications:
FC
Additional Info:
The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor (extracellular epitope), expressed on T cell subsets and some other cell types, such as macrophages.
The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
CD8 positive Wistar rat splenic T cell hybridomas
Applications:
FC
Additional Info:
The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor (extracellular epitope), expressed on T cell subsets and some other cell types, such as macrophages.
The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
High Mw glycoproteins from rat thymocytes
Applications:
WB,IHC,FA,FC,IP
Additional Info:
The mouse monoclonal antibody OX-8 recognizes the hinge-like membrane-proximal extracellular domain of rat CD8a (32-34 kDa; alpha chain of the CD8 antigen).
The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
High Mw glycoproteins from rat thymocytes
Applications:
FC,IP,WB,IHC
Additional Info:
The mouse monoclonal antibody OX-8 recognizes the hinge-like membrane-proximal extracellular domain of rat CD8a (32-34 kDa; alpha chain of the CD8 antigen).
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
MLR generated rat Th cells
Applications:
FC,IHC,ICC
Additional Info:
The mouse monoclonal antibody OX-35 reacts with an extracellular epitope of rat CD4 transmembrane glycoprotein (55 kDa).
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
MLR generated rat Th cells
Applications:
FC
Additional Info:
The mouse monoclonal antibody OX-35 reacts with an extracellular epitope of rat CD4 transmembrane glycoprotein (55 kDa).
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
MLR generated rat Th cells
Applications:
FC
Additional Info:
The mouse monoclonal antibody OX-35 reacts with an extracellular epitope of rat CD4 transmembrane glycoprotein (55 kDa).
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
MLR generated rat Th cells
Applications:
FC
Additional Info:
The mouse monoclonal antibody OX-35 reacts with an extracellular epitope of rat CD4 transmembrane glycoprotein (55 kDa).
CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mouse A20J B lymphoma cells transfected with rat CD28
Applications:
FA,FC,IP
Additional Info:
The mouse monoclonal antibody JJ319 reacts with an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mouse A20J B lymphoma cells transfected with rat CD28
Applications:
FC,IP
Additional Info:
The mouse monoclonal antibody JJ319 reacts with an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Mouse A20J B lymphoma cells transfected with rat CD28
Applications:
FC
Additional Info:
The mouse monoclonal antibody JJ319 reacts with an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Mouse A20J B lymphoma cells transfected with rat CD28
Applications:
FC
Additional Info:
The mouse monoclonal antibody JJ319 reacts with an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
CD161, also known as Nkrp1 (natural killer receptor protein 1) or Klrb1 (killer cell lectin-like receptor subfamily b member 1), is a disulphide-linked homodimeric receptor, which is involved in regulation of NK cell and NKT cell function. It is expressed on rat NK cells, subset of T cells, dendritic cells, and activated monocytes. Although human CD161 is expressed as one isoform, the rat CD161 has three isoforms, referred to as CD161a, b, and c. These proteins contain C-terminal C-type lectin extracellular domain, a transmembrane domain, and N-terminal intracellular domain, which contains ITIM motif, such as CD161b, and displays inhibitory function, or does not contain ITIM motif, thus also not the inhibitory function, such as CD161a.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Splenic cells purified from the LEW rat
Applications:
FC,IP,WB,IHC,RIA
Additional Info:
The mouse monoclonal antibody 10/78 recognizes CD161, an approximately 30 kDa type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer. A common extracellular epitope on rat CD161a and b isoforms is detected.
CD161, also known as Nkrp1 (natural killer receptor protein 1) or Klrb1 (killer cell lectin-like receptor subfamily b member 1), is a disulphide-linked homodimeric receptor, which is involved in regulation of NK cell and NKT cell function. It is expressed on rat NK cells, subset of T cells, dendritic cells, and activated monocytes. Although human CD161 is expressed as one isoform, the rat CD161 has three isoforms, referred to as CD161a, b, and c. These proteins contain C-terminal C-type lectin extracellular domain, a transmembrane domain, and N-terminal intracellular domain, which contains ITIM motif, such as CD161b, and displays inhibitory function, or does not contain ITIM motif, thus also not the inhibitory function, such as CD161a.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Splenic cells purified from the LEW rat
Applications:
FC
Additional Info:
The mouse monoclonal antibody 10/78 recognizes CD161, an approximately 30 kDa type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer. A common extracellular epitope on rat CD161a and b isoforms is detected.
CD161, also known as Nkrp1 (natural killer receptor protein 1) or Klrb1 (killer cell lectin-like receptor subfamily b member 1), is a disulphide-linked homodimeric receptor, which is involved in regulation of NK cell and NKT cell function. It is expressed on rat NK cells, subset of T cells, dendritic cells, and activated monocytes. Although human CD161 is expressed as one isoform, the rat CD161 has three isoforms, referred to as CD161a, b, and c. These proteins contain C-terminal C-type lectin extracellular domain, a transmembrane domain, and N-terminal intracellular domain, which contains ITIM motif, such as CD161b, and displays inhibitory function, or does not contain ITIM motif, thus also not the inhibitory function, such as CD161a.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Splenic cells purified from the LEW rat
Applications:
FC
Additional Info:
The mouse monoclonal antibody 10/78 recognizes CD161, an approximately 30 kDa type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer. A common extracellular epitope on rat CD161a and b isoforms is detected.
R-Ras2 / TC21 is the only member of R-Ras family of small GTPases that shows transforming activities similar to H-Ras, N-Ras, and K-Ras, and it is also structurally similar to them. R-Ras2 seems to play an important role in activating signal transduction pathways that control cell proliferation. Its mutations are associated with the growth of certain tumors, but also overexpression of the wild type form of R-Ras2 has been frequently detected in various carcinomas. Pseudogenes of R-Ras2 gene are found on chromosomes 1 and 2. Alternate splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Human TC21
Applications:
FC,WB,ELISA,ICC
Additional Info:
The mouse monoclonal antibody EM-50 recognizes R-Ras2 / TC21 protein (intracellular antigen) and does not react with R-Ras1, H-Ras, K-Ras, and N-Ras.
R-Ras2 / TC21 is the only member of R-Ras family of small GTPases that shows transforming activities similar to H-Ras, N-Ras, and K-Ras, and it is also structurally similar to them. R-Ras2 seems to play an important role in activating signal transduction pathways that control cell proliferation. Its mutations are associated with the growth of certain tumors, but also overexpression of the wild type form of R-Ras2 has been frequently detected in various carcinomas. Pseudogenes of R-Ras2 gene are found on chromosomes 1 and 2. Alternate splicing results in multiple transcript variants.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Human TC21
Applications:
FC
Additional Info:
The mouse monoclonal antibody EM-50 recognizes R-Ras2 / TC21 protein (intracellular antigen) and does not react with R-Ras1, H-Ras, K-Ras, and N-Ras.
Ribosomal protein SA (RPSA) is a multi-functional protein, that is an important component of 40S ribosomal subunit, and binds to lamin. Higher expression of RPSA is characteristic for many carcinomas, and correlates with their invasivity and metastatic potential. It has also been described, that RPSA interacts with amyloid beta peptide during Alzheimer´s disease.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
REPRLLVVTDPRADHQP
Applications:
WB,IHC,ICC,ELISA,IP,FC
Additional Info:
The mouse monoclonal antibody RP-01 recognizes ribosomal protein SA (RPSA), which is important for formation and stability of 40S ribosomal subunit, and is overexpressed in many carcinomas.
RLTPR / CARMIL2 (RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing; capping protein regulator and myosin 1 linker 2), also known as LRRC16C, is a cytosolic protein, which with high affinity binds CAPZA2 (capping protein muscle actin Z-line alpha 2) and decreases CAPZA2 affinity for actin barbed ends. RLTPR / CARMIL2 increases the rate of actin filament elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Its interaction with CAPZA2 is essential for lamellipodial protrusion and cell translocation. RLTPR / CARMIL2 is crutial for T cell costimulation via CD28 and this property seems to be independent on its actin-uncapping function. The lack of functional RLTPR / CARMIL2 molecules impeded the differentiation toward Th1 and Th17 fates of both human and murine CD4+ T cells and leads to combined immunodeficiency. Expression of RLTPR / CARMIL2 was also detected in human and murine B cells, but it seems not to be involved in BCR-mediated signaling.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Murine RLTPR
Applications:
FC,WB
Additional Info:
The mouse monoclonal antibody EM-53 recognizes RLTPR / CARMIL2, an intracellular protein playing a role in actin filament elongation.
RLTPR / CARMIL2 (RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing; capping protein regulator and myosin 1 linker 2), also known as LRRC16C, is a cytosolic protein, which with high affinity binds CAPZA2 (capping protein muscle actin Z-line alpha 2) and decreases CAPZA2 affinity for actin barbed ends. RLTPR / CARMIL2 increases the rate of actin filament elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. Its interaction with CAPZA2 is essential for lamellipodial protrusion and cell translocation. RLTPR / CARMIL2 is crutial for T cell costimulation via CD28 and this property seems to be independent on its actin-uncapping function. The lack of functional RLTPR / CARMIL2 molecules impeded the differentiation toward Th1 and Th17 fates of both human and murine CD4+ T cells and leads to combined immunodeficiency. Expression of RLTPR / CARMIL2 was also detected in human and murine B cells, but it seems not to be involved in BCR-mediated signaling.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Murine RLTPR
Applications:
FC
Additional Info:
The mouse monoclonal antibody EM-53 recognizes RLTPR / CARMIL2, an intracellular protein playing a role in actin filament elongation.
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
CD80-transfected CHO cell line
Applications:
IHC,IP,FC,FA
Additional Info:
The Armenian hamster monoclonal antibody 16-10A1 reacts with an extracellular epitope of CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
CD80-transfected CHO cell line
Applications:
FC,IP,IHC
Additional Info:
The Armenian hamster monoclonal antibody 16-10A1 reacts with an extracellular epitope of CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
C3H/eb mouse B cell lymphoma 38C-13
Applications:
FC,IP,IHC,ICC,FA
Additional Info:
The rat monoclonal antibody MEL-14 reacts with an extracellular epitope of murine CD62L (L-selectin), a 75 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.
CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
C3H/eb mouse B cell lymphoma 38C-13
Applications:
FC,IP,IHC,ICC
Additional Info:
The rat monoclonal antibody MEL-14 reacts with an extracellular epitope of murine CD62L (L-selectin), a 75 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.
CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
C3H/eb mouse B cell lymphoma 38C-13
Applications:
FC
Additional Info:
The rat monoclonal antibody MEL-14 reacts with an extracellular epitope of murine CD62L (L-selectin), a 75 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.
CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells it seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (rheumatoid arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
murine thymus or spleen cells
Applications:
FC,IP,IHC
Additional Info:
The rat monoclonal antibody 53-7.3 recognizes an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B-1 lymphocytes.
CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells it seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (rheumatoid arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
murine thymus or spleen cells
Applications:
FC
Additional Info:
The rat monoclonal antibody 53-7.3 recognizes an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B-1 lymphocytes.
CD49e, the alpha 5 integrin, noncovalently associates with the beta 1integrin (CD29) to form VLA-5 integrin complex. CD49e itself is composed of two disulfide-linked chains of 135 kDa and 25 kDa. VLA-5 binds to RGD sequence of fibronectin, and to neural adhesion molecule L1. It is important in maintaining the integrity of endothelial monolayers, in monocyte migration into the extracellular tissues, and it also provides a co-stimulatory signal to T cells and enhances receptor and complement receptor-mediated phagocytosis.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
C57BL/6 x A/J)F1 murine mast cell line
Applications:
ICC,FA,FC,IHC
Additional Info:
The rat monoclonal antibody 5H10-27 (MFR5) recognizes an extracellular epitope of murine CD49e, a 135 kDa protein serving as VLA-5 alpha chain, expressed on thymocytes, activated T cells, splenic B cells, and mast cells.
CD49e, the alpha 5 integrin, noncovalently associates with the beta 1integrin (CD29) to form VLA-5 integrin complex. CD49e itself is composed of two disulfide-linked chains of 135 kDa and 25 kDa. VLA-5 binds to RGD sequence of fibronectin, and to neural adhesion molecule L1. It is important in maintaining the integrity of endothelial monolayers, in monocyte migration into the extracellular tissues, and it also provides a co-stimulatory signal to T cells and enhances receptor and complement receptor-mediated phagocytosis.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
C57BL/6 x A/J)F1 murine mast cell line
Applications:
ICC,FC,IHC
Additional Info:
The rat monoclonal antibody 5H10-27 (MFR5) recognizes an extracellular epitope of murine CD49e, a 135 kDa protein serving as VLA-5 alpha chain, expressed on thymocytes, activated T cells, splenic B cells, and mast cells.
CD49e, the alpha 5 integrin, noncovalently associates with the beta 1integrin (CD29) to form VLA-5 integrin complex. CD49e itself is composed of two disulfide-linked chains of 135 kDa and 25 kDa. VLA-5 binds to RGD sequence of fibronectin, and to neural adhesion molecule L1. It is important in maintaining the integrity of endothelial monolayers, in monocyte migration into the extracellular tissues, and it also provides a co-stimulatory signal to T cells and enhances receptor and complement receptor-mediated phagocytosis.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
C57BL/6 x A/J)F1 murine mast cell line
Applications:
FC
Additional Info:
The rat monoclonal antibody 5H10-27 (MFR5) recognizes an extracellular epitope of murine CD49e, a 135 kDa protein serving as VLA-5 alpha chain, expressed on thymocytes, activated T cells, splenic B cells, and mast cells.
CD49e, the alpha 5 integrin, noncovalently associates with the beta 1integrin (CD29) to form VLA-5 integrin complex. CD49e itself is composed of two disulfide-linked chains of 135 kDa and 25 kDa. VLA-5 binds to RGD sequence of fibronectin, and to neural adhesion molecule L1. It is important in maintaining the integrity of endothelial monolayers, in monocyte migration into the extracellular tissues, and it also provides a co-stimulatory signal to T cells and enhances receptor and complement receptor-mediated phagocytosis.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
C57BL/6 x A/J)F1 murine mast cell line
Applications:
FC
Additional Info:
The rat monoclonal antibody 5H10-27 (MFR5) recognizes an extracellular epitope of murine CD49e, a 135 kDa protein serving as VLA-5 alpha chain, expressed on thymocytes, activated T cells, splenic B cells, and mast cells.
CD49d (integrin alpha 4), also known as very late antigen 4 alpha (VLA-4 alpha) is a cell surface glycoprotein constituting integrin complexes. CD49d heterodimerizes with CD29 (integrin beta 1) to form VLA-4 antigen, which is involved in cell-cell and cell-extracellular matrix interactions, which is a receptor for CD106 (VCAM) and fibronectin. CD79d also heterodimerizes with integrin beta 7 to form LPAM-1, which binds to MAdCAM-1 (mucosal vascular addressin). These interactions are important for cell adhesion and activation.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
murine T lymphoma line TK1
Applications:
FC,IP,IHC
Additional Info:
The rat monoclonal antibody R1-2 recognizes an extracellular epitope of murine CD49d / Integrin alpha 4 (VLA-4 alpha), an approximately 150 kDa glycoprotein of the integrin family, expressed on multiple blood cell types.
CD49d (integrin alpha 4), also known as very late antigen 4 alpha (VLA-4 alpha) is a cell surface glycoprotein constituting integrin complexes. CD49d heterodimerizes with CD29 (integrin beta 1) to form VLA-4 antigen, which is involved in cell-cell and cell-extracellular matrix interactions, which is a receptor for CD106 (VCAM) and fibronectin. CD79d also heterodimerizes with integrin beta 7 to form LPAM-1, which binds to MAdCAM-1 (mucosal vascular addressin). These interactions are important for cell adhesion and activation.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
murine T lymphoma line TK1
Applications:
FC
Additional Info:
The rat monoclonal antibody R1-2 recognizes an extracellular epitope of murine CD49d / Integrin alpha 4 (VLA-4 alpha), an approximately 150 kDa glycoprotein of the integrin family, expressed on multiple blood cell types.
CD45R, also known as B220, is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.
The rat monoclonal antibody RA3-6B2 recognizes an extracellular epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.
Clone number:
RA3-6B2
Antibody Isotype:
IgG2a k
Application Details:
Functional application: Modulation of B cell responses. Flow cytometry: Recommended dilution: 1-4 µg/ml
CD45R, also known as B220, is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.
The rat monoclonal antibody RA3-6B2 recognizes an extracellular epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.
CD45R, also known as B220, is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
The rat monoclonal antibody RA3-6B2 recognizes an extracellular epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.
CD45R, also known as B220, is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
The rat monoclonal antibody RA3-6B2 recognizes an extracellular epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.
CD45R, also known as B220, is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
The rat monoclonal antibody RA3-6B2 recognizes an extracellular epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Murine peripheral blood leukocytes
Applications:
FC,IP,WB,ICC
Additional Info:
The rat monoclonal antibody EM-05 reacts with an extracellular epitope of murine CD45 antigen (Leukocyte Common Antigen), a single chain type I transmembrane protein expressed at high level on cells of hematopoietic origin, except erythrocytes and platelets.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Murine peripheral blood leukocytes
Applications:
FC
Additional Info:
The rat monoclonal antibody EM-05 reacts with an extracellular epitope of murine CD45 antigen (Leukocyte Common Antigen), a single chain type I transmembrane protein expressed at high level on cells of hematopoietic origin, except erythrocytes and platelets.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Murine peripheral blood leukocytes
Applications:
FC
Additional Info:
The rat monoclonal antibody EM-05 reacts with an extracellular epitope of murine CD45 antigen (Leukocyte Common Antigen), a single chain type I transmembrane protein expressed at high level on cells of hematopoietic origin, except erythrocytes and platelets.
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Murine peripheral blood leukocytes
Applications:
FC
Additional Info:
The rat monoclonal antibody EM-05 reacts with an extracellular epitope of murine CD45 antigen (Leukocyte Common Antigen), a single chain type I transmembrane protein expressed at high level on cells of hematopoietic origin, except erythrocytes and platelets.
CD41 (platelet glycoprotein IIb) is composed of two subunits (120 kDa a, alpha and 23 kDa b, beta) that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during early stages of hematopoietic differentiation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Murine platelets
Applications:
FC,IP,IHC,ELISA,FA
Additional Info:
The rat monoclonal antibody MWReg30 recognizes an extracellular epitope of CD41 (GPIIb), a transmembrane glycoprotein (integrin family) composed of two chains GPIIb alpha (heavy chain; 120 kDa) and GPIIb beta (light chain; 23 kDa). CD41 is mainly expressed on platelets and megakaryocytes.
CD41 (platelet glycoprotein IIb) is composed of two subunits (120 kDa a, alpha and 23 kDa b, beta) that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during early stages of hematopoietic differentiation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Murine platelets
Applications:
FC,IP,IHC,ELISA
Additional Info:
The rat monoclonal antibody MWReg30 recognizes an extracellular epitope of CD41 (GPIIb), a transmembrane glycoprotein (integrin family) composed of two chains GPIIb alpha (heavy chain; 120 kDa) and GPIIb beta (light chain; 23 kDa). CD41 is mainly expressed on platelets and megakaryocytes.
CD41 (platelet glycoprotein IIb) is composed of two subunits (120 kDa a, alpha and 23 kDa b, beta) that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during early stages of hematopoietic differentiation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Murine platelets
Applications:
FC
Additional Info:
The rat monoclonal antibody MWReg30 recognizes an extracellular epitope of CD41 (GPIIb), a transmembrane glycoprotein (integrin family) composed of two chains GPIIb alpha (heavy chain; 120 kDa) and GPIIb beta (light chain; 23 kDa). CD41 is mainly expressed on platelets and megakaryocytes.
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mouse CTL clone V4 cells
Applications:
FC,IP,IHC,ICC,FA
Additional Info:
The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
Clone number:
GK1.5
Antibody Isotype:
IgG2b
Application Details:
Functional application: Isolation and depletion of CD4+ T cells, blocking of ligand binding to CD4. Immunocytochemistry: Recommended dilution: 1-4 ?g/ml. Immunoprecipitation: Recommended dilution: 1-2 ?g / 100-500 ?g of protein in 1 ml lysate. Flow cytometry: Recommended dilution: 1 ?g/million cells.
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mouse CTL clone V4 cells
Applications:
FC,IP,IHC,ICC,FA
Additional Info:
The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
Clone number:
GK1.5
Antibody Isotype:
IgG2b
Application Details:
Functional application: Isolation and depletion of CD4+ T cells, blocking of ligand binding to CD4. Immunocytochemistry: Recommended dilution: 1-4 ?g/ml. Immunoprecipitation: Recommended dilution: 1-2 ?g / 100-500 ?g of protein in 1 ml lysate. Flow cytometry: Recommended dilution: 1 ?g/million cells. Immunohistochemistry: Recommended dilution: 5-10 ?g/ml.
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mouse CTL clone V4 cells
Applications:
FC,IP,IHC,ICC
Additional Info:
The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
Clone number:
GK1.5
Antibody Isotype:
IgG2b
Application Details:
Immunocytochemistry: Recommended dilution: 1-4 ?g/ml. Immunoprecipitation: Recommended dilution: 1-2 ?g / 100-500 ?g of protein in 1 ml lysate. Flow cytometry: Recommended dilution: 1 ?g/million cells.
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Mouse CTL clone V4 cells
Applications:
FC
Additional Info:
The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Mouse CTL clone V4 cells
Applications:
FC
Additional Info:
The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Mouse CTL clone V4 cells
Applications:
FC
Additional Info:
The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mouse BM10-37 cytotoxic T lymphocytes
Applications:
FC,IP,IHC,ICC,FA
Additional Info:
The Armenian hamster monoclonal antibody 145-2C11 reacts with an extracellular epitope of murine CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for murine T cells.
Clone number:
145-2C11
Antibody Isotype:
IgG k
Application Details:
Functional application: Induction of T cell activation, proliferation or apoptosis (depending on conditions), in vivo T cell depletion. Flow cytometry: Recommended dilution: 1-2 ?g / ml (million cells). Immunoprecipitation: Recommended dilution: 1-2 ?g / 100-500 ?g protein in 1 ml of a cell lysate.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mouse BM10-37 cytotoxic T lymphocytes
Applications:
FC,IP,IHC,ICC
Additional Info:
The Armenian hamster monoclonal antibody 145-2C11 reacts with an extracellular epitope of murine CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for murine T cells.
Clone number:
145-2C11
Antibody Isotype:
IgG k
Application Details:
Flow cytometry: Recommended dilution: 1-2 ?g / ml (million cells). Immunoprecipitation: Recommended dilution: 1-2 ?g / 100-500 ?g protein in 1 ml of a cell lysate.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Mouse BM10-37 cytotoxic T lymphocytes
Applications:
FC
Additional Info:
The Armenian hamster monoclonal antibody 145-2C11 reacts with an extracellular epitope of murine CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for murine T cells.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Mouse BM10-37 cytotoxic T lymphocytes
Applications:
FC
Additional Info:
The Armenian hamster monoclonal antibody 145-2C11 reacts with an extracellular epitope of murine CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for murine T cells.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Mouse BM10-37 cytotoxic T lymphocytes
Applications:
FC
Additional Info:
The Armenian hamster monoclonal antibody 145-2C11 reacts with an extracellular epitope of murine CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for murine T cells.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mouse BM10-37 cytotoxic T lymphocytes
Applications:
FC,IP,IHC,ICC
Additional Info:
The Armenian hamster monoclonal antibody 145-2C11 reacts with an extracellular epitope of murine CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for murine T cells.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Mouse BM10-37 cytotoxic T lymphocytes
Applications:
FC
Additional Info:
The Armenian hamster monoclonal antibody 145-2C11 reacts with an extracellular epitope of murine CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for murine T cells.
CD26, also known as dipeptidyl peptidase IV (DPP-IV), is a homodimeric cell surface serine peptidase that degradates IFN-gamma-induced cytokines, acts as a T cell costimulatory molecule, and participates in multiple immunopathological roles in leukocyte homing and inflammation. Alterations in its peptidase activity are characteristic of malignant transformation. The enzymatic activity increases dramatically with tumour grade and severity. CD26 is expressed in various blood cell types, but also e.g. in cells that are histogenetically related to activated fibroblasts. Alterations in CD26 density have been reported on circulating monocytes and CD4+ T cells during rheumatoid arthritis and systemic lupus erythematosus.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
BALB/c thymocytes
Applications:
IP,FC,IHC
Additional Info:
The rat monoclonal antibody H194-112 recognizes an extracellular epitope of CD26, a 110 kDa type II membrane glycoprotein, which is a peptidase expressed on mature thymocytes, T cells (especially activated), B cells, NK cells and macrophages.
CD26, also known as dipeptidyl peptidase IV (DPP-IV), is a homodimeric cell surface serine peptidase that degradates IFN-gamma-induced cytokines, acts as a T cell costimulatory molecule, and participates in multiple immunopathological roles in leukocyte homing and inflammation. Alterations in its peptidase activity are characteristic of malignant transformation. The enzymatic activity increases dramatically with tumour grade and severity. CD26 is expressed in various blood cell types, but also e.g. in cells that are histogenetically related to activated fibroblasts. Alterations in CD26 density have been reported on circulating monocytes and CD4+ T cells during rheumatoid arthritis and systemic lupus erythematosus.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
BALB/c thymocytes
Applications:
FC
Additional Info:
The rat monoclonal antibody H194-112 recognizes an extracellular epitope of CD26, a 110 kDa type II membrane glycoprotein, which is a peptidase expressed on mature thymocytes, T cells (especially activated), B cells, NK cells and macrophages.
CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
murine thymocytes
Applications:
FA,FC,IP
Additional Info:
The rat monoclonal antibody RM2-5 recognizes an extracellular epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T lymphocytes and NK cells; also expressed by all thymocytes.
CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
murine thymocytes
Applications:
FC,IP
Additional Info:
The rat monoclonal antibody RM2-5 recognizes an extracellular epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T lymphocytes and NK cells; also expressed by all thymocytes.
CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
murine thymocytes
Applications:
FC
Additional Info:
The rat monoclonal antibody RM2-5 recognizes an extracellular epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T lymphocytes and NK cells; also expressed by all thymocytes.
CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
murine thymocytes
Applications:
FC
Additional Info:
The rat monoclonal antibody RM2-5 recognizes an extracellular epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T lymphocytes and NK cells; also expressed by all thymocytes.
CD197 / CCR7 (chemokine receptor 7) is a seven membrane-spanning protein serving as a receptor for chemokines CCL19 and CCL21. It is expressed on most naive T cells, some hematopoietic stem cells, mature dendritic cells, NK cells and some memory T cells and B cells subsets. CD197 plays important roles in development of thymocytes, and regulating the recirculation and homing of lymphocytes and dendritic cells to secondary lymphoid organs.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Murine CD197-transfected RBL-2H3 cells
Applications:
FC,IP
Additional Info:
The rat monoclonal antibody 4B12 recognizes an extracellular epitope of murine CD197 / CCR7 (chemokine receptor 7), a 43 kDa G-protein-coupled receptor for chemokines CCL19 and CCL21.
CD197 / CCR7 (chemokine receptor 7) is a seven membrane-spanning protein serving as a receptor for chemokines CCL19 and CCL21. It is expressed on most naive T cells, some hematopoietic stem cells, mature dendritic cells, NK cells and some memory T cells and B cells subsets. CD197 plays important roles in development of thymocytes, and regulating the recirculation and homing of lymphocytes and dendritic cells to secondary lymphoid organs.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Murine CD197-transfected RBL-2H3 cells
Applications:
FC
Additional Info:
The rat monoclonal antibody 4B12 recognizes an extracellular epitope of murine CD197 / CCR7 (chemokine receptor 7), a 43 kDa G-protein-coupled receptor for chemokines CCL19 and CCL21.
CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mouse CD19-transfected cell line
Applications:
FC,IP,IHC,FA
Additional Info:
The rat monoclonal antibody 1D3 detects an extracellular epitope of murine CD19, 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.
Clone number:
1D3
Antibody Isotype:
IgG2a
Application Details:
Functional application: This antibody can induce down-regulation of CD19, affecting the proportions of B cell subpopulations. Flow cytometry: Recommended dilution: 1-4 µg/ml
CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mouse CD19-transfected cell line
Applications:
FC,IP,IHC
Additional Info:
The rat monoclonal antibody 1D3 detects an extracellular epitope of murine CD19, 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.
CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Mouse CD19-transfected cell line
Applications:
FC
Additional Info:
The rat monoclonal antibody 1D3 detects an extracellular epitope of murine CD19, 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.
CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Murine cytotoxic T cell glycoproteins
Applications:
FC,IP,WB,IHC,FA
Additional Info:
The rat monoclonal antibody M18/2 recognizes an extracellular epitope of CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.
CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Murine cytotoxic T cell glycoproteins
Applications:
FC,IP,WB,IHC
Additional Info:
The rat monoclonal antibody M18/2 recognizes an extracellular epitope of CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.
CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Murine cytotoxic T cell glycoproteins
Applications:
FC
Additional Info:
The rat monoclonal antibody M18/2 recognizes an extracellular epitope of CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.
CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Unlike human, the murine protein is expressed only as a transmembrane isoform. Also CD32 (FcgammaRII) is a low affinity receptor for IgG, but its affinity is lower than that of CD16. These receptors are expressed on monocytes/macrophages, NK cells, granulocytes, mast cells, dendritic cells, and B cells. Their role is to mediate adaptive immune responses through binding the antibody-antigen immunocomplexes, but their effect on the particular cell differs according to the cell type.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Murine pre-B cells
Applications:
FC,IP
Additional Info:
The rat monoclonal antibody 93 recognizes a common extracellular epitope of murine CD16 (FcgammaRIII) and CD32 (FcgammaRII), the low affinity receptors for IgG.
CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Unlike human, the murine protein is expressed only as a transmembrane isoform. Also CD32 (FcgammaRII) is a low affinity receptor for IgG, but its affinity is lower than that of CD16. These receptors are expressed on monocytes/macrophages, NK cells, granulocytes, mast cells, dendritic cells, and B cells. Their role is to mediate adaptive immune responses through binding the antibody-antigen immunocomplexes, but their effect on the particular cell differs according to the cell type.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Murine pre-B cells
Applications:
FC
Additional Info:
The rat monoclonal antibody 93 recognizes a common extracellular epitope of murine CD16 (FcgammaRIII) and CD32 (FcgammaRII), the low affinity receptors for IgG.
CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Unlike human, the murine protein is expressed only as a transmembrane isoform. Also CD32 (FcgammaRII) is a low affinity receptor for IgG, but its affinity is lower than that of CD16. These receptors are expressed on monocytes/macrophages, NK cells, granulocytes, mast cells, dendritic cells, and B cells. Their role is to mediate adaptive immune responses through binding the antibody-antigen immunocomplexes, but their effect on the particular cell differs according to the cell type.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Murine pre-B cells
Applications:
FC
Additional Info:
The rat monoclonal antibody 93 recognizes a common extracellular epitope of murine CD16 (FcgammaRIII) and CD32 (FcgammaRII), the low affinity receptors for IgG.
CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Activated mouse Th1 clone D1.6
Applications:
FC,IP,IHC,ICC,FA
Additional Info:
The Armenian hamster monoclonal antibody MR-1 detects an extracellular epitope on murine CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.
CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Activated mouse Th1 clone D1.6
Applications:
FC,IP,IHC,ICC
Additional Info:
The Armenian hamster monoclonal antibody MR-1 detects an extracellular epitope on murine CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.
CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Activated mouse Th1 clone D1.6
Applications:
FC
Additional Info:
The Armenian hamster monoclonal antibody MR-1 detects an extracellular epitope on murine CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.
CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
B10 mouse spleen cells enriched for T cells
Applications:
FC,IP,IHC,FA
Additional Info:
The rat monoclonal antibody M1/70 detects an extracellular epitope of CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
Clone number:
M1/70
Antibody Isotype:
IgG2b
Application Details:
Functional application: In vitro blocking of CD11b. Flow cytometry: Recommended dilution: 1 ?g/ml.
CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
B10 mouse spleen cells enriched for T cells
Applications:
FC,IP,IHC
Additional Info:
The rat monoclonal antibody M1/70 detects an extracellular epitope of CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
B10 mouse spleen cells enriched for T cells
Applications:
FC
Additional Info:
The rat monoclonal antibody M1/70 detects an extracellular epitope of CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
B10 mouse spleen cells enriched for T cells
Applications:
FC
Additional Info:
The rat monoclonal antibody M1/70 detects an extracellular epitope of CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
B10 mouse spleen cells enriched for T cells
Applications:
FC
Additional Info:
The rat monoclonal antibody M1/70 detects an extracellular epitope of CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
C57BL/6 mouse splenic secondary cytotoxic T lymphocytes
Applications:
FC,IP,IHC,FA
Additional Info:
The rat monoclonal antibody M17/4 reacts with an extracellular epitope of CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
C57BL/6 mouse splenic secondary cytotoxic T lymphocytes
Applications:
FC,IP,IHC
Additional Info:
The rat monoclonal antibody M17/4 reacts with an extracellular epitope of CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
C57BL/6 mouse splenic secondary cytotoxic T lymphocytes
Applications:
FC
Additional Info:
The rat monoclonal antibody M17/4 reacts with an extracellular epitope of CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
CD106 / VCAM-1 (vascular cell adhesion molecule-1) is an Ig-like cell surface adhesion molecule binding VLA-4 integrin. VCAM-1 is a potent T cell costimulatory molecule taking part in their positive selection and survival, as well as in adhesion, transendothelial migration and activation of peripheral T cells. VCAM-1 is also involved in endothelial cell-cell contacts. Whereas VCAM-1 normally mediates leukocyte extravasion to sites of tissue inflammation, tumour cells can use overexpressed VCAM-1 to escape T cell immunity.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Murine preadipose cell line PA6
Applications:
FC,IP,IHC,FA
Additional Info:
The rat monoclonal antibody 429 (also known as MVCAM.A) recognizes an extracellular epitope of murine CD106, a 100-110 kDa type I membrane protein of the immunoglobulin superfamily, a crucial mediator of leukocyte adhesion, and a costimulation molecule.
CD106 / VCAM-1 (vascular cell adhesion molecule-1) is an Ig-like cell surface adhesion molecule binding VLA-4 integrin. VCAM-1 is a potent T cell costimulatory molecule taking part in their positive selection and survival, as well as in adhesion, transendothelial migration and activation of peripheral T cells. VCAM-1 is also involved in endothelial cell-cell contacts. Whereas VCAM-1 normally mediates leukocyte extravasion to sites of tissue inflammation, tumour cells can use overexpressed VCAM-1 to escape T cell immunity.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Murine preadipose cell line PA6
Applications:
FC,IP,IHC
Additional Info:
The rat monoclonal antibody 429 (also known as MVCAM.A) recognizes an extracellular epitope of murine CD106, a 100-110 kDa type I membrane protein of the immunoglobulin superfamily, a crucial mediator of leukocyte adhesion, and a costimulation molecule.
CD106 / VCAM-1 (vascular cell adhesion molecule-1) is an Ig-like cell surface adhesion molecule binding VLA-4 integrin. VCAM-1 is a potent T cell costimulatory molecule taking part in their positive selection and survival, as well as in adhesion, transendothelial migration and activation of peripheral T cells. VCAM-1 is also involved in endothelial cell-cell contacts. Whereas VCAM-1 normally mediates leukocyte extravasion to sites of tissue inflammation, tumour cells can use overexpressed VCAM-1 to escape T cell immunity.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Murine preadipose cell line PA6
Applications:
FC
Additional Info:
The rat monoclonal antibody 429 (also known as MVCAM.A) recognizes an extracellular epitope of murine CD106, a 100-110 kDa type I membrane protein of the immunoglobulin superfamily, a crucial mediator of leukocyte adhesion, and a costimulation molecule.
CD106 / VCAM-1 (vascular cell adhesion molecule-1) is an Ig-like cell surface adhesion molecule binding VLA-4 integrin. VCAM-1 is a potent T cell costimulatory molecule taking part in their positive selection and survival, as well as in adhesion, transendothelial migration and activation of peripheral T cells. VCAM-1 is also involved in endothelial cell-cell contacts. Whereas VCAM-1 normally mediates leukocyte extravasion to sites of tissue inflammation, tumour cells can use overexpressed VCAM-1 to escape T cell immunity.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Murine preadipose cell line PA6
Applications:
FC
Additional Info:
The rat monoclonal antibody 429 (also known as MVCAM.A) recognizes an extracellular epitope of murine CD106, a 100-110 kDa type I membrane protein of the immunoglobulin superfamily, a crucial mediator of leukocyte adhesion, and a costimulation molecule.
CD105 (endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Inflamed mouse skin
Applications:
FC,IP,WB,IHC
Additional Info:
The rat monoclonal antibody MJ7/18 reacts with an extracellular epitope of CD105 (Endoglin), a 90 kDa type I homodimerizing membrane glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow.
CD105 (endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Inflamed mouse skin
Applications:
FC
Additional Info:
The rat monoclonal antibody MJ7/18 reacts with an extracellular epitope of CD105 (Endoglin), a 90 kDa type I homodimerizing membrane glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow.
SLP65 / BLNK (SH2 domain-containing leukocyte-specific phosphoprotein of 65 kDa; B cell linker protein), also known as BASH, is an adaptor protein that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). Phosphorylated by Syk tyrosine kinase, SLP65 serves as a scaffold for Btk tyrosine kinase, Vav1 guanine nucleotide exchange factor, phospholipase C gamma2, as well as Grb2 and Nck adaptor proteins; thus represents a central linker protein that bridges the BCR-associated kinases with a multitude of signaling pathways.
Product Type:
Antibodies Primary
Antibody Type:
polyclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
A fusion protein representing amino acids 171-356 of human BLNK.
Applications:
WB
Additional Info:
The polyclonal antibody reacts with mouse SLP65 / BLNK, a cytosolic adaptor protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human SLP65).
The mPlum is a red fluorescent protein with excitation maximum 589 nm and emission maximum 650 nm. It has around 29 kDa, and it is being used as a fluorescent tag in expression systems.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
mPlum from Discosoma sp.
Applications:
WB,ICC,FC
Clone number:
PAb (919)
Application Details:
Flow cytometry: Recommended dilution: 1-4 ?g/ml, extracellular staining or intracellular staining - depending on expression.Western blotting: Recommended dilution: 1-2 ?g/ml.
MICA and MICB glycoproteins are members of MHC class I family, closely linked to HLA-B. However, unlike HLA molecules, MICA and MICB are not associated with beta2 microglobulin and are conformationally stable in the absence of conventional MHC class I peptide ligands. Both proteins are stress-induced antigens expressed mainly in gastrointestinal epithelium, where they are recognized by V-delta1 subset of gamma/delta T cells, and also on diverse epithelial tumor cells. Binding of MICA/MICB receptor, the NKG2D, leads to cytolytic response of NK cells, Tc cells, and gamma/delta T cells. Alternative splicing results in multiple isoforms, and some of them have been associated with susceptibility to psoriasis and psoriatic arthritis. Shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Transfected C1R cells expressing MICA
Applications:
FC,IP,IHC
Additional Info:
The mouse monoclonal antibody 6D4 recognizes a common extracellular epitope on MICA and MICB glycoproteins, transmembrane ligands of NKG2D, and is able to block NKG2D-mediated activation of NK cells and cytotoxic T cells.
MICA and MICB glycoproteins are members of MHC class I family, closely linked to HLA-B. However, unlike HLA molecules, MICA and MICB are not associated with beta2 microglobulin and are conformationally stable in the absence of conventional MHC class I peptide ligands. Both proteins are stress-induced antigens expressed mainly in gastrointestinal epithelium, where they are recognized by V-delta1 subset of gamma/delta T cells, and also on diverse epithelial tumor cells. Binding of MICA/MICB receptor, the NKG2D, leads to cytolytic response of NK cells, Tc cells, and gamma/delta T cells. Alternative splicing results in multiple isoforms, and some of them have been associated with susceptibility to psoriasis and psoriatic arthritis. Shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Transfected C1R cells expressing MICA
Applications:
FC
Additional Info:
The mouse monoclonal antibody 6D4 recognizes a common extracellular epitope on MICA and MICB glycoproteins, transmembrane ligands of NKG2D, and is able to block NKG2D-mediated activation of NK cells and cytotoxic T cells.
MICA and MICB glycoproteins are members of MHC class I family, closely linked to HLA-B. However, unlike HLA molecules, MICA and MICB are not associated with beta2 microglobulin and are conformationally stable in the absence of conventional MHC class I peptide ligands. Both proteins are stress-induced antigens expressed mainly in gastrointestinal epithelium, where they are recognized by V-delta1 subset of gamma/delta T cells, and also on diverse epithelial tumor cells. Binding of MICA/MICB receptor, the NKG2D, leads to cytolytic response of NK cells, Tc cells, and gamma/delta T cells. Alternative splicing results in multiple isoforms, and some of them have been associated with susceptibility to psoriasis and psoriatic arthritis. Shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Transfected C1R cells expressing MICA
Applications:
FC
Additional Info:
The mouse monoclonal antibody 6D4 recognizes a common extracellular epitope on MICA and MICB glycoproteins, transmembrane ligands of NKG2D, and is able to block NKG2D-mediated activation of NK cells and cytotoxic T cells.
MICA and MICB glycoproteins are members of MHC class I family, closely linked to HLA-B. However, unlike HLA molecules, MICA and MICB are not associated with beta2 microglobulin and are conformationally stable in the absence of conventional MHC class I peptide ligands. Both proteins are stress-induced antigens expressed mainly in gastrointestinal epithelium, where they are recognized by V-delta1 subset of gamma/delta T cells, and also on diverse epithelial tumor cells. Binding of MICA/MICB receptor, the NKG2D, leads to cytolytic response of NK cells, Tc cells, and gamma/delta T cells. Alternative splicing results in multiple isoforms, and some of them have been associated with susceptibility to psoriasis and psoriatic arthritis. Shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Transfected C1R cells expressing MICA
Applications:
FC
Additional Info:
The mouse monoclonal antibody 6D4 recognizes a common extracellular epitope on MICA and MICB glycoproteins, transmembrane ligands of NKG2D, and is able to block NKG2D-mediated activation of NK cells and cytotoxic T cells.
MICA and MICB glycoproteins are members of MHC class I family, closely linked to HLA-B. However, unlike HLA molecules, MICA and MICB are not associated with beta2 microglobulin and are conformationally stable in the absence of conventional MHC class I peptide ligands. Both proteins are stress-induced antigens expressed mainly in gastrointestinal epithelium, where they are recognized by V-delta1 subset of gamma/delta T cells, and also on diverse epithelial tumor cells. Binding of MICA/MICB receptor, the NKG2D, leads to cytolytic response of NK cells, Tc cells, and gamma/delta T cells. Alternative splicing results in multiple isoforms, and some of them have been associated with susceptibility to psoriasis and psoriatic arthritis. Shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Transfected C1R cells expressing MICA
Applications:
FC,IP,ICC
Additional Info:
The mouse monoclonal antibody 6D4 recognizes a common extracellular epitope on MICA and MICB glycoproteins, transmembrane ligands of NKG2D, and is able to block NKG2D-mediated activation of NK cells and cytotoxic T cells.
MICA and MICB glycoproteins are members of MHC class I family, closely linked to HLA-B. However, unlike HLA molecules, MICA and MICB are not associated with beta2 microglobulin and are conformationally stable in the absence of conventional MHC class I peptide ligands. Both proteins are stress-induced antigens expressed mainly in gastrointestinal epithelium, where they are recognized by V-delta1 subset of gamma/delta T cells, and also on diverse epithelial tumor cells. Binding of MICA/MICB receptor, the NKG2D, leads to cytolytic response of NK cells, Tc cells, and gamma/delta T cells. Alternative splicing results in multiple isoforms, and some of them have been associated with susceptibility to psoriasis and psoriatic arthritis. Shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Transfected C1R cells expressing MICA
Applications:
FC
Additional Info:
The mouse monoclonal antibody 6D4 recognizes a common extracellular epitope on MICA and MICB glycoproteins, transmembrane ligands of NKG2D, and is able to block NKG2D-mediated activation of NK cells and cytotoxic T cells.
The mCherry is a red fluorescent protein with excitation maximum 587 nm and emission maximum 610 nm. It has around 28 kDa, and it is being used as a fluorescent tag in expression systems.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
mCherry protein from Anaplasma marginale
Applications:
WB,ICC,FC
Clone number:
PAb (918)
Application Details:
Flow cytometry: Recommended dilution: 1-4 ?g/ml, extracellular staining or intracellular staining - depending on expression.Western blotting: Recommended dilution: 1-2 ?g/ml.
MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Microtubule protein (bovine brain) enriched for kinesin
Applications:
IP,WB,IHC,ICC,ELISA
Additional Info:
The antibody MT-07 recognizes an epitope (aa 1375-1395) located in central domain of molecule Microtubule Associated Protein 2ab (MAP2ab), an intracellular antigen.
MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Microtubule protein (bovine brain) enriched for kinesin
Applications:
IP,WB,IHC,ICC,ELISA
Additional Info:
The antibody MT-08 recognizes an epitope (aa 1375-1395) located in central domain of molecule Microtubule Associated Protein 2ab (MAP2ab), an intracellular antigen.
MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Termostabile fraction of bovine brain microtubules (whole MAP2ab molecule).
Applications:
IP,WB,ICC
Additional Info:
The antibody MT-01 reacts with Microtubule Associated Protein 2ab (MAP2ab), an intracellular antigen.
Lysozyme is anti-bacterial enzyme found mainly in milk, saliva, tears, plasma, spleen, mucus, and leukocytes (e.g. in cytoplasmic granules of neutrophils). It damages bacterial cell walls by hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. Lysozyme is part of the innate immune system. It protects wet body surfaces, such as conjunctiva. Reduced lysozyme levels have been associated with bronchopulmonary dysplasia in newborns. On the other hand high lysozyme blood levels produced for example by myelomonocytic leukemia cells can lead to kidney failure and low blood potassium.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
human lysozyme
Applications:
FC,ICC
Additional Info:
The mouse monoclonal antibody LZ598-10G9 recognizes lysozyme, an approximately 17 kDa antibacterial enzyme, which is being used as a marker for the lineage diagnosis of acute leukemias (intracellular antigen).
Lysozyme is anti-bacterial enzyme found mainly in milk, saliva, tears, plasma, spleen, mucus, and leukocytes (e.g. in cytoplasmic granules of neutrophils). It damages bacterial cell walls by hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. Lysozyme is part of the innate immune system. It protects wet body surfaces, such as conjunctiva. Reduced lysozyme levels have been associated with bronchopulmonary dysplasia in newborns. On the other hand high lysozyme blood levels produced for example by myelomonocytic leukemia cells can lead to kidney failure and low blood potassium.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
human lysozyme
Applications:
FC
Additional Info:
The mouse monoclonal antibody LZ598-10G9 recognizes lysozyme, an approximately 17 kDa antibacterial enzyme, which is being used as a marker for the lineage diagnosis of acute leukemias (intracellular antigen).
Lysozyme is anti-bacterial enzyme found mainly in milk, saliva, tears, plasma, spleen, mucus, and leukocytes (e.g. in cytoplasmic granules of neutrophils). It damages bacterial cell walls by hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. Lysozyme is part of the innate immune system. It protects wet body surfaces, such as conjunctiva. Reduced lysozyme levels have been associated with bronchopulmonary dysplasia in newborns. On the other hand high lysozyme blood levels produced for example by myelomonocytic leukemia cells can lead to kidney failure and low blood potassium.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
human lysozyme
Applications:
FC
Additional Info:
The mouse monoclonal antibody LZ598-10G9 recognizes lysozyme, an approximately 17 kDa antibacterial enzyme, which is being used as a marker for the lineage diagnosis of acute leukemias (intracellular antigen).
Lyn is a Src-family protein tyrosine kinase that is predominantly expressed in hematopoietic cells. It is associated with a number of cell surface receptors including the B cell antigen receptor (BCR) and Fc receptors. Upon their triggering, Lyn phosphorylates subunits of these receptors in a cholesterol-dependent manner, utilizing the plasma membrane lipid raft system. The phosphorylated intracellular domains of the receptors are accessible for cytoplasmic Syk tyrosine kinase, which is activated by Lyn-mediated phosphorylation and which transduces the signal to downstream adaptors. Lyn is abnormally distributed in acute myeloid leukemia cells and seems to be a novel pharmacologic target of this disease.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Bacterially expressed recombinant fragment of human Lyn (aa 8 - 238).
Applications:
IP,WB,ICC
Additional Info:
The antibody LYN-01 reacts with Lyn (p56/p53), a non-receptor Src-family tyrosine kinase expressed in hematopoietic tissues (intracellular antigen).
Clone number:
LYN-01
Antibody Isotype:
IgG1
Application Details:
Western blotting: Recommended dilution: 1-2 ?g/ml.
LST1 (leukocyte-specific transcript 1, also known as B144) is expressed in cells of myeloid/erythroid lineage (monocytes, granulocytes, dendritic cells, platelets, erythrocytes). At least 14 alternatively spliced variants (LST1/A – LST1/N) can be detected; some of them (LST1/A, B, C, G, I, K) are transmembrane cell surface-exposed forms, the other are soluble. LST1 induces production of long, thin filopodia in dendritic cells, has an inhibitory effect on lymphocyte proliferation and may have an immunomodulatory role. LST1/A is an 11 kDa transmembrane adaptor present in membrane rafts and forms spontaneously covalent homodimers. Its intracellular domain contains two tyrosine motifs, one of them being an ITIM very similar to such motifs in Siglec.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
KLH-fused peptide corresponding to amino acids SSEGPDLRGRDKRGT of human LST1
Applications:
IP,WB,ICC
Additional Info:
The antibody LST1/02 reacts with an extracellular epitope of LST1, an approximately 6-11 kDa protein expressed as various transmembrane or soluble forms. LST1 is found predominantly on monocytes and dendritic cells. It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.
Clone number:
LST1/02
Antibody Isotype:
IgG1
Application Details:
Immunoprecipitation: Positive control: RAJI human Burkitt lymphoma cell line. Western blotting: The antigen migrates on SDS PAGE gels as approximately 25-28 kDa molecule.
GARP (glycoprotein A repetitions predominant protein), also known as garpin or LRRC32 (leucin-rich repeat containing protein 32) is an approximately 80 kDa transmembrane glycoprotein detected on the surface of megakaryocytes, platelets, and activated Treg cells. It binds to the latency-associated peptide (LAP) domain of pro-TGF beta and regulates its storage and activation. The expression of GARP on Treg cells seems to be necessary for their suppressive functions.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Purified human sGARP protein
Applications:
WB
Additional Info:
The mouse monoclonal antibody GARP5 recognizes GARP / LRRC32, an approximately 80 kDa glycoprotein expressed e.g. on the surface of megakaryocytes, platelets and activated Treg cells.
Clone number:
GARP5
Antibody Isotype:
IgG1
Application Details:
Western blotting: Recommended dilution: 1-2 ?g/ml; positive control: human thrombocytes, reducing conditions
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.
Applications:
IP,FC
Additional Info:
The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
Product Type:
Antibodies Primary
Antibody Type:
polyclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.
Applications:
WB,IHC
Additional Info:
The polyclonal antibody reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin.
Applications:
WB,IHC
Additional Info:
The antibody LIME-10 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.
Applications:
FC
Additional Info:
The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.
Lck is a Src-family tyrosine kinase, which is essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, Lck phosphorylates the ITAM motives in its zeta subunits, establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by Lck and thereby activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. Whereas the majority of Lck is localized to the plasma membrane, there is also a significant fraction associated with the Golgi apparatus, which may contribute to Raf activation under conditions of weak stimulation through the TCR. Lck is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Peptide corresponding to amino acids 22-36 in the sequence of human Lck.
Applications:
FC,IP,WB,ICC
Additional Info:
The antibody LCK-01 recognizes defined epitope (aa 22-36) of Lck, a 56 kDa Src-family protein tyrosine kinase (intracellular antigen).
LAT (linker for activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Bacterially produced recombinant polypeptide
corresponding to the entire cytoplasmic domain of human LAT.
Applications:
WB,IP,FC
Additional Info:
The antibody LAT-01 reacts with an intracellular epitope of LAT, a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.
Clone number:
LAT-01
Antibody Isotype:
IgG1
Application Details:
Immunoprecipitation: Tyrosine-phosphorylated form of LAT is not optimally recognized. Flow cytometry: Intracellular staining. Tyrosine-phosphorylated form of LAT is not optimally recognized.Western blotting: Recommended dilution: 1-2 ?g/ml.
LARGE1 serves as a glycosyltransferase which participates in glycosylation of the muscle membrane protein alpha-dystroglycan. Mutations of LARGE1 lead to hypoglycosylation of alpha-dystroglycan and cause congenital muscular dystrophy (MDC1D) associated with severe mental retardation. Altered alpha-dystroglycan glycosylation may also play a role in cancer, as hypoglycosylation of the protein and loss of laminin binding have been demonstrated in invasive carcinoma cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant fragment of human LARGE1 (amino acids 35-142)
Applications:
FC,WB
Additional Info:
The mouse monoclonal antibody LARGE-02 recognizes human LARGE1, a glycosyltransferase expressed mainly in the Golgi apparatus. Crossreactivity with LARGE2 was not determined.
LARGE1 serves as a glycosyltransferase which participates in glycosylation of the muscle membrane protein alpha-dystroglycan. Mutations of LARGE1 lead to hypoglycosylation of alpha-dystroglycan and cause congenital muscular dystrophy (MDC1D) associated with severe mental retardation. Altered alpha-dystroglycan glycosylation may also play a role in cancer, as hypoglycosylation of the protein and loss of laminin binding have been demonstrated in invasive carcinoma cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Recombinant fragment of human LARGE1 (amino acids 35-142)
Applications:
FC
Additional Info:
The mouse monoclonal antibody LARGE-02 recognizes human LARGE1, a glycosyltransferase expressed mainly in the Golgi apparatus. Crossreactivity with LARGE2 was not determined.
Lactoferrin is an iron-binding glycoprotein of the transferrin family, which is released to most biological fluids, with particularly high levels in milk. It has anti-inflammatory (e.g. sequestering of lipopolysaccharides), anti-microbial (e.g. blocking of viral attachment to the target cell), and immunomodulatory properties and can prevent infections in young children. Lactoferrin is considered to bridge the innate and adaptive immune responses. It also participates in iron homeostasis, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Besides biological fluids it is also found in the secondary granules of neutrophils.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
human lactoferrin
Applications:
FC,ELISA
Additional Info:
The mouse monoclonal antibody LF5-1D2 recognizes lactoferrin, an iron-binding secreted glycoprotein of about 90 kDa, which has anti-inflammatory, immunomodulatory and anti-microbial properties.
Lactoferrin is an iron-binding glycoprotein of the transferrin family, which is released to most biological fluids, with particularly high levels in milk. It has anti-inflammatory (e.g. sequestering of lipopolysaccharides), anti-microbial (e.g. blocking of viral attachment to the target cell), and immunomodulatory properties and can prevent infections in young children. Lactoferrin is considered to bridge the innate and adaptive immune responses. It also participates in iron homeostasis, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Besides biological fluids it is also found in the secondary granules of neutrophils.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
human lactoferrin
Applications:
FC
Additional Info:
The mouse monoclonal antibody LF5-1D2 recognizes lactoferrin, an iron-binding secreted glycoprotein of about 90 kDa, which has anti-inflammatory, immunomodulatory and anti-microbial properties.
Clone number:
LF5-1D2
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
Ku Antigen (DNA-dependent DNA helicase) is a heterodimer (of 72 and 87 kDa polypeptides) which contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the nonhomologous end-joining pathway. A DNA double-strand break is a major lesion that destroys the integrity of the DNA molecule. Such damage is introduced by ionizing radiation. Ku binds to free double-stranded DNA ends and is the DNA-binding component of the DNA-dependent protein kinase. Thus, the Ku protein is involved in DNA repair and in V(D)J recombination, and the Ku-DNA-dependent protein kinase complex may have a role in those same processes. Ku70 and Ku80 share a common topology and form a dyad-symmetrical molecule with a preformed ring that encircles duplex DNA. The binding site can cradle 2 full turns of DNA while encircling only the central 3-4 base pairs. Ku makes no contacts with DNA bases and few with the sugar-phosphate backbone, but it fits sterically to major and minor groove contours so as to position the DNA helix in a defined path through the protein ring. These features are well designed to structurally support broken DNA ends and to bring the DNA helix into phase across the junction during end processing and ligation. Mouse cells deficient for Ku80 display a marked increase in chromosomal aberrations, including breakage, translocations, and aneuploidy. Despite the observed chromosome instabilities, Ku80 -/- mice have only a slightly earlier onset of cancer. Loss of p53 synergizes with Ku80 to promote tumorigenesis such that all Ku80 -/- and p53 -/- mice succumb to disseminated pro-B-cell lymphoma before 3 months of age. The p70/p80 complex binds to the ends of double-stranded DNA in a cell cycle-dependent manner, being associated with chromosomes of interphase cells, followed by complete dissociation from the condensing chromosomes in early prophase. Some patients with systemic lupus erythematosus produce very large amounts of autoantibodies to p70 and p80. The autoantibody has been found in patients with autoimmune thyroid disease (Graves disease) as well as in those with lupus.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
human whole T-lymphocytes
Applications:
ICC,IP
Additional Info:
The antibody MEM-54 reacts with Ku80, a 80 kDa subunit of Ku autoantigen (heterodimer of 72 and 87 kDa intracellular polypeptides). Ku autoantigen is involved in DNA repair and in V(D)J recombination through its ability to bind DNA double-strand breaks.
Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.
Product Type:
Antibodies Primary
Antibody Type:
polyclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
stalk domain of human kinesin (aa 331-906) expressed in E. coli (FKHC3)
Applications:
WB,ICC
Additional Info:
The polyclonal antibody detects total level of endogenous kinesin protein (intracellular antigen).
Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Enriched fraction of porcine brain kinesin.
Applications:
ICC
Additional Info:
The antibody KN-03 recognizes heavy chain of conventional kinesin, a protein associated with intracellular vesicles, and with lower affinity with denaturated molecule. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments The antibody KN-03 well recognizes kinesin bound to taxol-stabilized microtubules.
Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Enriched fraction of porcine brain kinesin.
Applications:
ICC
Additional Info:
The antibody KN-02 recognizes heavy chain of conventional kinesin, a protein associated with intracellular vesicles, and with lower affinity with denaturated molecule. Epitope is located in coiled-coil stalk domain. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody KN-02 does not react with kinesin bound to taxol-stabilized microtubules.
Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Nuclei of the Hodgkin lymphoma cell line L428
Applications:
FC,WB,IHC,ICC
Additional Info:
The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Nuclei of the Hodgkin lymphoma cell line L428
Applications:
FC
Additional Info:
The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
Clone number:
Ki-67
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.
Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Nuclei of the Hodgkin lymphoma cell line L428
Applications:
FC
Additional Info:
The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
Clone number:
Ki-67
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
Insulin and glucagon are pancreatic endocrine hormones secreted by islet cells within the pancreas. The stimulus for insulin secretion is a HIGH blood glucose. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Porcine insulin.
Applications:
ELISA,RIA,IHC,ICC,FA
Additional Info:
The antibody IN-05 reacts with insulin, one of the major regulatory endocrine hormones of intermediate metabolism, normally secreted by the beta cells (a type of islet cells) of the pancreas; it is also present in tumors of B cell origin such as insulinoma.
Clone number:
IN-05
Antibody Isotype:
IgG1
Application Details:
Functional application: The antibody IN-05 blocks binding of insulin to the receptor.
Insulin and glucagon are pancreatic endocrine hormones secreted by islet cells within the pancreas. The stimulus for insulin secretion is a HIGH blood glucose. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Porcine insulin.
Applications:
ELISA,RIA,IHC,ICC
Additional Info:
The antibody IN-05 reacts with insulin, one of the major regulatory endocrine hormones of intermediate metabolism, normally secreted by the beta cells (a type of islet cells) of the pancreas; it is also present in tumors of B cell origin such as insulinoma.
Ikaros, also known as IKZF1 (Ikaros family zinc finger protein 1) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Helios. Ikaros forms homo- and heterodimers with these proteins and functions predominantly in early hematopoietic development. Expression of Ikaros, Aiolos and Helios is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Disruption of Ikaros leads to T and B cell leukemias.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant human Ikaros (C-terminal part)
Applications:
FC,IP,WB,ICC
Additional Info:
The mouse monoclonal antibody 4E9 recognizes Ikaros, a transcription factor (intracellular antigen) expressed broadly in hematopoietic progenitors and serving as a key regulator of lymphopoiesis.
Ikaros, also known as IKZF1 (Ikaros family zinc finger protein 1) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Helios. Ikaros forms homo- and heterodimers with these proteins and functions predominantly in early hematopoietic development. Expression of Ikaros, Aiolos and Helios is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Disruption of Ikaros leads to T and B cell leukemias.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Recombinant human Ikaros (C-terminal part)
Applications:
FC
Additional Info:
The mouse monoclonal antibody 4E9 recognizes Ikaros, a transcription factor (intracellular antigen) expressed broadly in hematopoietic progenitors and serving as a key regulator of lymphopoiesis.
Clone number:
4000000000
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
The interferon gamma (IFN-gamma; 16-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12; its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN. IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant human interferon gamma
Applications:
IP,ELISA,RIA
Additional Info:
The mouse monoclonal antibody NIB42 recognizes IFN-gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. Binds both glycosylated and non-glycosylated protein.
Clone number:
NIB42
Antibody Isotype:
IgG1 k
Application Details:
ELISA: Capture antibody in combination with detection antibody 4S.B3.
The interferon gamma (IFN-gamma; 16-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12; its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN. IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Interferon gamma derived from human leukocytes
Applications:
FC,IP,WB,IHC,ICC,ELISA,RIA
Additional Info:
The mouse monoclonal antibody 4S.B3 recognizes IFN-gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. Binds both glycosylated and non-glycosylated protein.
Clone number:
4S.B3
Antibody Isotype:
IgG1 k
Application Details:
ELISA: This antibody is being used as detection antibody in combination with capture antibody NIB42. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
The interferon gamma (IFN-gamma; 16-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12; its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN. IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant human IFN-gamma (aa 22-166 representing mature IFN-gamma)
Applications:
FC,WB,ELISA
Additional Info:
The mouse monoclonal antibody G-23 reacts with IFN-gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells.
The interferon gamma (IFN-gamma; 16-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12; its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN. IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Interferon gamma derived from human leukocytes
Applications:
FC
Additional Info:
The mouse monoclonal antibody 4S.B3 recognizes IFN-gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. Binds both glycosylated and non-glycosylated protein.
Clone number:
4S.B3
Antibody Isotype:
IgG1 k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
The interferon gamma (IFN-gamma; 16-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12; its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN. IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Interferon gamma derived from human leukocytes
Applications:
FC
Additional Info:
The mouse monoclonal antibody 4S.B3 recognizes IFN-gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. Binds both glycosylated and non-glycosylated protein.
Clone number:
4S.B3
Antibody Isotype:
IgG1 k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.
The interferon gamma (IFN-gamma; 16-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12; its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN. IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Interferon gamma derived from human leukocytes
Applications:
FC,IP,WB,IHC,ICC,ELISA
Additional Info:
The mouse monoclonal antibody 4S.B3 recognizes IFN-gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. Binds both glycosylated and non-glycosylated protein.
The interferon gamma (IFN-gamma; 16-25 kDa) is an important regulator of the immune response, produced in activated Th1 cells and NK cells, particularly in response to IL-2, TNF-alpha and IL-12; its production is suppressed by IL-4, IL-10, and TGF-beta. The producing of IFN-gamma is activated by specific antigens or mitogens through the T cell antigen receptor. IFN-gamma polypeptide forms: 40-60 kDa forms are observable under non-denaturing conditions as dimers and trimers; 20 kDa and 25 kDa forms exist due to variable glycosylation. IFN-gamma belongs to the type II interferons, also called immune IFN. IFN-gamma shows antiviral activity and has important immunoregulatory functions. It is a potent activator of macrophages and had antiproliferative effects on transformed cells. IFN-gamma plays an important role in regulating B cell differentiation by simultaneously stimulating class switch recombination to the IgG3 and IgG2a isotypes while represing class switch recombination to the IgE and IgG1 isotypes. It also appears to promote antigen presentation by B cells through its effects on MHC. Binding of IFN-gamma to its receptor increases the expression of class I MHC on all somatic cells. It also enhances the expression of class II MHC on antigen-presenting cells. IFN-gamma is the major means by which T cells activate macrophages, increasing their ability to kill bacteria, parasites, and tumours. The activation of macrophages by IFN-gamma is essential for the elimination of bacteria that replicate within the phagosomes of macrophages (f.e. Mycobacteria and Listeria monocytogenes). IFN-gamma can potentiate the high antiviral and antitumor effects of the type I interferons (IFN-alpha, IFN-beta). IFN-gamma may also activate neutrophils and NK cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Interferon gamma derived from human leukocytes
Applications:
FC
Additional Info:
The mouse monoclonal antibody 4S.B3 recognizes IFN-gamma, a 16-25 kDa cytokine produced by activated Th1 cells and NK cells. Binds both glycosylated and non-glycosylated protein.
Clone number:
4S.B3
Antibody Isotype:
IgG1 k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
TROP2 is a cell surface receptor that transduces calcium signals. It belongs to carcinoma-associated antigens. Mutations of TROP2 have been associated with gelatinous drop-like corneal dystrophy.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
TROP2-transfected CHO cells
Applications:
WB,FC,IHC,ICC
Clone number:
TrMab-6
Antibody Isotype:
IgG2b k
Application Details:
Flow cytometry: Recommended dilution: 1-4 ?g/ml.Western blotting: Recommended dilution: 1 ?g/ml.Immunocytochemistry: Recommended dilution: 10 ?g/ml; the cells can be fixed with 4% PFA and permeabilized with 0.1% Triton X-100 before antibody staining, when needed. Immunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml. Heat-mediated antigen retrieval in citrate buffer, pH = 6.
TROP2 is a cell surface receptor that transduces calcium signals. It belongs to carcinoma-associated antigens. Mutations of TROP2 have been associated with gelatinous drop-like corneal dystrophy.
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
TROP2 is a cell surface receptor that transduces calcium signals. It belongs to carcinoma-associated antigens. Mutations of TROP2 have been associated with gelatinous drop-like corneal dystrophy.
TROP2 is a cell surface receptor that transduces calcium signals. It belongs to carcinoma-associated antigens. Mutations of TROP2 have been associated with gelatinous drop-like corneal dystrophy.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
TROP2-transfected CHO cells
Applications:
FC
Clone number:
TrMab-6
Antibody Isotype:
IgG2b k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Tomm34 (translocase of the outer mitochondrial membrane 34) is an additional component of the cellular chaperone system involved in protein folding. As the name suggests, Tomm34 was initially identified as being involved in mitochondrial protein processing. Subsequent studies have shown that Tomm34 interacts with both Hsp70 and Hsp90 and modifies their protein folding activities. In cancer, high levels of Tomm34 have been reported in bladder, colorectal and breast cancers compared to their normal tissue counterparts. In these cancers, Tomm34 promotes colorectal cancer cell growth and is a biomarker of poor outcome in early invasive breast cancer and bladder cancer. As a tumour-associated protein, Tomm34 peptide vaccination is under investigation as a therapeutic option for colorectal cancer, with significant Tomm34 cytotoxic T-lymphocyte (CTL) response observed.
TCR Vgamma9 is a variant of TCR gamma chain, that is present on a subset of human gamma/delta T cells. TCR Vgamma9/Vdelta2 T cells are able to recognize and kill various tumor cells, as this receptor heterodimer binds to certain phosphoantigens, expressed by tumors.
TCR Vgamma9 is a variant of TCR gamma chain, that is present on a subset of human gamma/delta T cells. TCR Vgamma9/Vdelta2 T cells are able to recognize and kill various tumor cells, as this receptor heterodimer binds to certain phosphoantigens, expressed by tumors.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
human T cells
Applications:
FC
Clone number:
B3
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
TCR Vgamma4 is a variant of TCR gamma chain, that is present on a minor subset of human gamma/delta T cells.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
human T cells
Applications:
FC
Clone number:
4A11.904
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
TCR Vdelta2 is a variant of TCR delta chain, that is present on a major subset of human gamma/delta T cells. TCR Vgamma9/Vdelta2 (or Vgamma2/Vdelta2) T cells are able to recognize and kill various tumor cells, as this receptor heterodimer binds to certain phosphoantigens, expressed by tumors. They can recognize these antigens in an MHC-unrestricted manner. Similarly to NK cells, Vdelta2 T cells express MHC I receptors and killer Ig-like receptors, that are involved in tumor recognition and cytolysis. The potently cytotoxic subset of them is identified by cell surface expression of polysialyated CD56.
TCR Vdelta2 is a variant of TCR delta chain, that is present on a major subset of human gamma/delta T cells. TCR Vgamma9/Vdelta2 (or Vgamma2/Vdelta2) T cells are able to recognize and kill various tumor cells, as this receptor heterodimer binds to certain phosphoantigens, expressed by tumors. They can recognize these antigens in an MHC-unrestricted manner. Similarly to NK cells, Vdelta2 T cells express MHC I receptors and killer Ig-like receptors, that are involved in tumor recognition and cytolysis. The potently cytotoxic subset of them is identified by cell surface expression of polysialyated CD56.
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
The antibody MEM-262 recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Human thymoma cell line HPB-ALL.
Applications:
FC,IP,WB,FA
Additional Info:
The antibody MEM-262 recognizes an extracellular epitope on beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This
subset is larger than that recognized by other V(beta5.3)-specific antibodies.
Clone number:
MEM-262
Antibody Isotype:
IgG2a
Application Details:
Western blotting: This antibody uniquely recognizes denatured TCR beta chains under the conditions of Western blotting. Functional application: This antibody activates T cells (V beta 5-related subset). Flow cytometry: Recommended dilution: 1-4 µg/ml
The antibody MEM-262 recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Human thymoma cell line HPB-ALL.
Applications:
FC,IP,WB,FA
Additional Info:
The antibody MEM-262 recognizes an extracellular epitope on beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This
subset is larger than that recognized by other V(beta5.3)-specific antibodies.
Clone number:
MEM-262
Antibody Isotype:
IgG2a
Application Details:
Western blotting: This antibody uniquely recognizes denatured TCR beta chains under the conditions of Western blotting. Functional application: This antibody activates T cells (V beta 5-related subset). Flow cytometry: Recommended dilution: 1-4 µg/ml
The antibody MEM-262 recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Human thymoma cell line HPB-ALL.
Applications:
FC,IP,WB
Additional Info:
The antibody MEM-262 recognizes an extracellular epitope on beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This
subset is larger than that recognized by other V(beta5.3)-specific antibodies.
Clone number:
MEM-262
Antibody Isotype:
IgG2a
Application Details:
Western blotting: This antibody uniquely recognizes denatured TCR beta chains under the conditions of Western blotting. Flow cytometry: Recommended dilution: 1-4 µg/ml
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC,IHC,FA
Additional Info:
The mouse monoclonal antibody B1 (also known as B1.1) recognizes an extracellular epitope of TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC,IHC
Additional Info:
The mouse monoclonal antibody B1 (also known as B1.1) recognizes an extracellular epitope of TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Additional Info:
The mouse monoclonal antibody B1 (also known as B1.1) recognizes an extracellular epitope of TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection.
Clone number:
B1
Antibody Isotype:
IgG1 k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Additional Info:
The mouse monoclonal antibody B1 (also known as B1.1) recognizes an extracellular epitope of TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection.
Clone number:
B1
Antibody Isotype:
IgG1 k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Additional Info:
The mouse monoclonal antibody B1 (also known as B1.1) recognizes an extracellular epitope of TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection.
Clone number:
B1
Antibody Isotype:
IgG1 k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Alpha-beta T cell receptors (TCRs) are antigen specific receptors, which are essential to the immune response and are present on the cell surface of T lymphocytes. They recognize peptide-loaded major histocompatibility complexes (pMHCs), that are displayed by antigen presenting cells (APCs). Binding of alpha-beta TCR to pMHC initiates TCR-CD3 clustering on the cell surface and intracellular activation of LCK, that phosphorylates the ITAM motifs of CD3gamma, CD3delta, CD3epsilon and CD3zeta, enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium signaling, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NFkappaB (NF-kB) pathways, leading to the mobilization of transcription factors, that are critical for gene expression and essential for T cell growth and differentiation. The T cell repertoire is generated by V-D-J-C rearrangements. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MHC restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TCRs with the pMHCs shapes TCR structural and functional avidity.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
thymus, spleen, and mesenteric lymph nodes isolated from a mouse transgenic for human TCR Vbeta3Cbeta1
Applications:
FC,IP,IHC
Additional Info:
The mouse monoclonal antibody JOVI.1 recognizes an extracellular epitope on TCR Cbeta1 (TRBC1).
Alpha-beta T cell receptors (TCRs) are antigen specific receptors, which are essential to the immune response and are present on the cell surface of T lymphocytes. They recognize peptide-loaded major histocompatibility complexes (pMHCs), that are displayed by antigen presenting cells (APCs). Binding of alpha-beta TCR to pMHC initiates TCR-CD3 clustering on the cell surface and intracellular activation of LCK, that phosphorylates the ITAM motifs of CD3gamma, CD3delta, CD3epsilon and CD3zeta, enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium signaling, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NFkappaB (NF-kB) pathways, leading to the mobilization of transcription factors, that are critical for gene expression and essential for T cell growth and differentiation. The T cell repertoire is generated by V-D-J-C rearrangements. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MHC restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TCRs with the pMHCs shapes TCR structural and functional avidity.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
thymus, spleen, and mesenteric lymph nodes isolated from a mouse transgenic for human TCR Vbeta3Cbeta1
Applications:
FC
Additional Info:
The mouse monoclonal antibody JOVI.1 recognizes an extracellular epitope on TCR Cbeta1 (TRBC1).
Clone number:
JOVI.1
Antibody Isotype:
IgG2a k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Alpha-beta T cell receptors (TCRs) are antigen specific receptors, which are essential to the immune response and are present on the cell surface of T lymphocytes. They recognize peptide-loaded major histocompatibility complexes (pMHCs), that are displayed by antigen presenting cells (APCs). Binding of alpha-beta TCR to pMHC initiates TCR-CD3 clustering on the cell surface and intracellular activation of LCK, that phosphorylates the ITAM motifs of CD3gamma, CD3delta, CD3epsilon and CD3zeta, enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium signaling, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NFkappaB (NF-kB) pathways, leading to the mobilization of transcription factors, that are critical for gene expression and essential for T cell growth and differentiation. The T cell repertoire is generated by V-D-J-C rearrangements. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MHC restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TCRs with the pMHCs shapes TCR structural and functional avidity.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
thymus, spleen, and mesenteric lymph nodes isolated from a mouse transgenic for human TCR Vbeta3Cbeta1
Applications:
FC
Additional Info:
The mouse monoclonal antibody JOVI.1 recognizes an extracellular epitope on TCR Cbeta1 (TRBC1).
Clone number:
JOVI.1
Antibody Isotype:
IgG2a k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Alpha-beta T cell receptors (TCRs) are antigen specific receptors, which are essential to the immune response and are present on the cell surface of T lymphocytes. They recognize peptide-loaded major histocompatibility complexes (pMHCs), that are displayed by antigen presenting cells (APCs). Binding of alpha-beta TCR to pMHC initiates TCR-CD3 clustering on the cell surface and intracellular activation of LCK, that phosphorylates the ITAM motifs of CD3gamma, CD3delta, CD3epsilon and CD3zeta, enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium signaling, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NFkappaB (NF-kB) pathways, leading to the mobilization of transcription factors, that are critical for gene expression and essential for T cell growth and differentiation. The T cell repertoire is generated by V-D-J-C rearrangements. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MHC restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TCRs with the pMHCs shapes TCR structural and functional avidity.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
thymus, spleen, and mesenteric lymph nodes isolated from a mouse transgenic for human TCR Vbeta3Cbeta1
Applications:
FC
Additional Info:
The mouse monoclonal antibody JOVI.1 recognizes an extracellular epitope on TCR Cbeta1 (TRBC1).
Clone number:
JOVI.1
Antibody Isotype:
IgG2a k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC
Additional Info:
The mouse monoclonal antibody IP26 recognizes a monomorphic extracellular determinant of TCR alpha/beta, the dominant subtype of T cell receptor expressed in human peripheral blood.
Clone number:
IP26
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: Recommended dilution: 2-4 ?g/ml; positive control: human peripheral blood T cells.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Additional Info:
The mouse monoclonal antibody IP26 recognizes a monomorphic extracellular determinant of TCR alpha/beta, the dominant subtype of T cell receptor expressed in human peripheral blood.
Clone number:
IP26
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Clone number:
IP26
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Additional Info:
The mouse monoclonal antibody IP26 recognizes a monomorphic extracellular determinant of TCR alpha/beta, the dominant subtype of T cell receptor expressed in human peripheral blood.
Clone number:
IP26
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Additional Info:
The mouse monoclonal antibody IP26 recognizes a monomorphic extracellular determinant of TCR alpha/beta, the dominant subtype of T cell receptor expressed in human peripheral blood.
Clone number:
IP26
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
FC
Additional Info:
The mouse monoclonal antibody IP26 recognizes a monomorphic extracellular determinant of TCR alpha/beta, the dominant subtype of T cell receptor expressed in human peripheral blood.
Clone number:
IP26
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: Recommended dilution: 2-3 ?g/ml; positive control: human peripheral blood T cells.
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Applications:
FC
Additional Info:
The mouse monoclonal antibody IP26 recognizes a monomorphic extracellular determinant of TCR alpha/beta, the dominant subtype of T cell receptor expressed in human peripheral blood.
Clone number:
IP26
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
SUSD2 (sushi domain containing protein 2) is a type I transmembrane protein, that serves as an important marker of bone marrow-derived mesenchymal stem-like cells (bone marrow stromal cells). These pluripotent cells are important for techniques of autologous cell therapy, and can be collected from e.g. endometrium, or palatine tonsil. SUSD2 seems to be a tumor supresor, and is down-regulated in colon cancer tissues, whereas it is highly expressed e.g. in breast cancer.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
WERI-RB-1 retinoblastoma cells
Applications:
FC
Additional Info:
The mouse monoclonal antibody W5C5 recognizes an extracellular epitope of SUSD2, a type I transmembrane protein expressed on mesenchymal stem-like cells. This antibody selectively binds to a MSCs in both bone marrow and endometrium or tonsil, and can be used for their identification and isolation.
SUSD2 (sushi domain containing protein 2) is a type I transmembrane protein, that serves as an important marker of bone marrow-derived mesenchymal stem-like cells (bone marrow stromal cells). These pluripotent cells are important for techniques of autologous cell therapy, and can be collected from e.g. endometrium, or palatine tonsil. SUSD2 seems to be a tumor supresor, and is down-regulated in colon cancer tissues, whereas it is highly expressed e.g. in breast cancer.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
WERI-RB-1 retinoblastoma cells
Applications:
FC
Additional Info:
The mouse monoclonal antibody W5C5 recognizes an extracellular epitope of SUSD2, a type I transmembrane protein expressed on mesenchymal stem-like cells. This antibody selectively binds to a MSCs in both bone marrow and endometrium or tonsil, and can be used for their identification and isolation.
SUSD2 (sushi domain containing protein 2) is a type I transmembrane protein, that serves as an important marker of bone marrow-derived mesenchymal stem-like cells (bone marrow stromal cells). These pluripotent cells are important for techniques of autologous cell therapy, and can be collected from e.g. endometrium, or palatine tonsil. SUSD2 seems to be a tumor supresor, and is down-regulated in colon cancer tissues, whereas it is highly expressed e.g. in breast cancer.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
WERI-RB-1 retinoblastoma cells
Applications:
ICC,FC
Additional Info:
The mouse monoclonal antibody W5C5 recognizes an extracellular epitope of SUSD2, a type I transmembrane protein expressed on mesenchymal stem-like cells. This antibody selectively binds to a MSCs in both bone marrow and endometrium or tonsil, and can be used for their identification and isolation.
STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Human CD34 positive bone marrow cells
Applications:
FC,ICC
Additional Info:
The mouse monoclonal antibody STRO-1 recognizes an extracellular epitope of the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.
STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Human CD34 positive bone marrow cells
Applications:
FC
Additional Info:
The mouse monoclonal antibody STRO-1 recognizes an extracellular epitope of the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.
STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Human CD34 positive bone marrow cells
Applications:
FC
Additional Info:
The mouse monoclonal antibody STRO-1 recognizes an extracellular epitope of the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.
STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.
SIGLEC8 is a sialic acid binding lectin similar to CD33. In its cytoplasmic comain it contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), and a motive similar to a binding site for SLAM-associated protein. SIGLEC8 is expressed e.g. in lymph nodes and spleen. It is an eosinophil marker, although it can be found also on the surface of mast cells. Crosslinking of SIGLEC8 leads to apoptosis. Soluble form of SIGLEC8 can be foud in human serum, especially in case of eosinophil-associated diseases.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Extracellular domain of human SIGLEC8 fused with Fc fragment of human IgG1
Applications:
FC,ELISA,IP
Additional Info:
The mouse monoclonal antibody 7C9 recognizes an extracellular epitope of SIGLEC8, an eosinophil marker, expressed e.g. in lymph nodes and spleen.
SIGLEC8 is a sialic acid binding lectin similar to CD33. In its cytoplasmic comain it contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), and a motive similar to a binding site for SLAM-associated protein. SIGLEC8 is expressed e.g. in lymph nodes and spleen. It is an eosinophil marker, although it can be found also on the surface of mast cells. Crosslinking of SIGLEC8 leads to apoptosis. Soluble form of SIGLEC8 can be foud in human serum, especially in case of eosinophil-associated diseases.
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Extracellular domain of human SIGLEC8 fused with Fc fragment of human IgG1
PODXL is a highly glycosylated sialomucin, which is expressed in many types of tumors, as well as it is a well known marker of embryonic stem cells. Overexpression of PODXL is an independent predictor of cancer progression, metastasis, and poor outcome. PODXL promotes tumor growts and invasiveness, and is a potential target for antibody therapy.
PDPN (podoplanin) is a type I transmembrane glycoprotein of mucin-type character. The specific function of this protein has not been determined, but its homologs in other species were described as differentiation antigens. PDPN can be used as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified.
The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P]D, corresponding to amino acids 378-393 of human p53.
Applications:
WB,IHC
Additional Info:
The mouse monoclonal antibody FP3.2 [FPS392] reacts with human p53 tumour suppressor intracellular protein phosphorylated at CKII site (Ser 392).
Clone number:
FP3.2 [FPS392]
Antibody Isotype:
IgG1
Application Details:
Immunohistochemistry (paraffin sections): Standard ABC technique (DAB+), pretreatment: high temperature antigen retrieval (microwave, pressure cooker) in 10 mM citrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0, recommended dilution: 10 ?g/ml, incubation: 1 hour at RT; or overnight at 4°C, positive tissue: breast carcinoma with high level of wild-type p53. Western blotting: recommended dilution: 1 ?g/ml.
NKp80, also known as CLEC5C or KLRF1, is a type II transmembrane glycoprotein of the C lectin family, which is expressed in 80 kDa homodimers on NK cells, and subsets of CD8+ alpha/beta T cells, and gamma/delta T cells. It belongs to the activating coreceptors, which induce cytotoxicity, and production of pro-inflammatory cytokines. Its ligand AICL is expressed on myeloid cells.
Myeloperoxidase (MPO) is a heme enzyme that is localized in azurophilic (primary) granules of myeloid cells and its synthesis occurs at an early stage of differentiation. The mature myeloperoxidase is a tetramer composed of two light (12 kDa) and two heavy (60 kDa) chains. This enzyme uses hydrogen peroxide to oxidize numerous substrates, including serotonin, melatonin or chloride, to produce reactive free radicals that contribute to immune reactions of myeloid cells against pathogens. Myeloperoxidase functions not only in host defense by mediating efficient microbial killing but also can contribute to progressive tissue damage in chronic inflammatory states such as atherosclerosis or acute pancreatitis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Human myeloperoxidase
Applications:
FC,ELISA
Additional Info:
The mouse monoclonal antibody MPO421-8B2 recognizes human myeloperoxidase, a heme protein present in intracellular granules of myeloblasts, neutrophils and monocytes. It is a marker of acute myelogenous leukemias and acute lymphoblastic leukemias.
Myeloperoxidase (MPO) is a heme enzyme that is localized in azurophilic (primary) granules of myeloid cells and its synthesis occurs at an early stage of differentiation. The mature myeloperoxidase is a tetramer composed of two light (12 kDa) and two heavy (60 kDa) chains. This enzyme uses hydrogen peroxide to oxidize numerous substrates, including serotonin, melatonin or chloride, to produce reactive free radicals that contribute to immune reactions of myeloid cells against pathogens. Myeloperoxidase functions not only in host defense by mediating efficient microbial killing but also can contribute to progressive tissue damage in chronic inflammatory states such as atherosclerosis or acute pancreatitis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Human myeloperoxidase
Applications:
FC
Additional Info:
The mouse monoclonal antibody MPO421-8B2 recognizes human myeloperoxidase, a heme protein present in intracellular granules of myeloblasts, neutrophils and monocytes. It is a marker of acute myelogenous leukemias and acute lymphoblastic leukemias.
Clone number:
MPO421-8B2
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
Myeloperoxidase (MPO) is a heme enzyme that is localized in azurophilic (primary) granules of myeloid cells and its synthesis occurs at an early stage of differentiation. The mature myeloperoxidase is a tetramer composed of two light (12 kDa) and two heavy (60 kDa) chains. This enzyme uses hydrogen peroxide to oxidize numerous substrates, including serotonin, melatonin or chloride, to produce reactive free radicals that contribute to immune reactions of myeloid cells against pathogens. Myeloperoxidase functions not only in host defense by mediating efficient microbial killing but also can contribute to progressive tissue damage in chronic inflammatory states such as atherosclerosis or acute pancreatitis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Human myeloperoxidase
Applications:
FC
Additional Info:
The mouse monoclonal antibody MPO421-8B2 recognizes human myeloperoxidase, a heme protein present in intracellular granules of myeloblasts, neutrophils and monocytes. It is a marker of acute myelogenous leukemias and acute lymphoblastic leukemias.
Clone number:
MPO421-8B2
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.
LOX1, a 31 kDa type II transmembrane protein, is a C-type lectin, functioning as a scavenger receptor for e.g. oxidized low density lipoprotein, apoptotic heat shock proteins, or CRP. It is expressed by macrophages, fibroblasts, platelets, endothelial cells, and smooth muscle cells, and its defects can lead to atherosclerosis. Its expression is enhanced under inflammatory conditions. Multiple splicing variants have been identified.
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
fusion protein of human LOX1 ectodomain and of human IgG Fc
LAR is a receptore-linked transmembrane protein tyrosine phosphatase expressed on mesenchymal stem cells, that reside e.g. in bone marrow, blood, placenta, adipose tissue, or skin, as well as it is expressed on some carcinoma cell lines, including HeLa, MCF-7, or HT29. During the process of externalization, LAR is intracellularly proteolytically processed into two non-covalently associated subunits. This protein is involved in intercellular and cell-matrix interactions and its extracellular part resembles that of cell adhesion molecules (CAMs). The extracellular part can be released from the surface, which may be used for regulation of LAR function.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
WERI-RB-1 retinoblastoma cells
Applications:
FC
Additional Info:
The mouse monoclonal antibody W7C6 recognizes an extracellular epitope of protein tyrosine phosphatase LAR, a marker of mesenchymal stem cells.
LAR is a receptore-linked transmembrane protein tyrosine phosphatase expressed on mesenchymal stem cells, that reside e.g. in bone marrow, blood, placenta, adipose tissue, or skin, as well as it is expressed on some carcinoma cell lines, including HeLa, MCF-7, or HT29. During the process of externalization, LAR is intracellularly proteolytically processed into two non-covalently associated subunits. This protein is involved in intercellular and cell-matrix interactions and its extracellular part resembles that of cell adhesion molecules (CAMs). The extracellular part can be released from the surface, which may be used for regulation of LAR function.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
WERI-RB-1 retinoblastoma cells
Applications:
FC
Additional Info:
The mouse monoclonal antibody W7C6 recognizes an extracellular epitope of protein tyrosine phosphatase LAR, a marker of mesenchymal stem cells.
LAR is a receptore-linked transmembrane protein tyrosine phosphatase expressed on mesenchymal stem cells, that reside e.g. in bone marrow, blood, placenta, adipose tissue, or skin, as well as it is expressed on some carcinoma cell lines, including HeLa, MCF-7, or HT29. During the process of externalization, LAR is intracellularly proteolytically processed into two non-covalently associated subunits. This protein is involved in intercellular and cell-matrix interactions and its extracellular part resembles that of cell adhesion molecules (CAMs). The extracellular part can be released from the surface, which may be used for regulation of LAR function.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
WERI-RB-1 retinoblastoma cells
Applications:
FC
Additional Info:
The mouse monoclonal antibody W7C6 recognizes an extracellular epitope of protein tyrosine phosphatase LAR, a marker of mesenchymal stem cells.
Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Purified human IgM.
Applications:
WB,ICC,ELISA,FC
Additional Info:
The antibody CH2 reacts with Fc fragment of human IgM.
Clone number:
CH2
Antibody Isotype:
IgG1
Application Details:
Western blotting: Recommended dilution: 1 ?g/ml.Flow cytometry: Recommended dilution: 1-4 ?g/ml, extracellular and intracellular staining.
Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
IgM from human serum.
Applications:
ELISA
Additional Info:
The antibody MA2 reacts with µ-chain of human IgM, Fab - region localized epitope.
Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Purified human IgM.
Applications:
FC
Additional Info:
The antibody CH2 reacts with Fc fragment of human IgM.
Clone number:
CH2
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: Recommended dilution: 1-4 ?g/ml. Extracellular and intracellular staining.
Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Purified human IgM.
Applications:
WB,ELISA
Additional Info:
The antibody CH2 reacts with Fc fragment of human IgM.
Clone number:
CH2
Antibody Isotype:
IgG1
Application Details:
Western blotting: Recommended dilution: 1 ?g/ml.Flow cytometry: Recommended dilution: 1-4 ?g/ml, extracellular and intracellular staining.
Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Purified human IgM.
Applications:
FC
Additional Info:
The antibody CH2 reacts with Fc fragment of human IgM.
Clone number:
CH2
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: Recommended dilution: 1-5 ?g/ml. Extracellular and intracellular staining.
Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Purified human IgM.
Applications:
FC
Additional Info:
The antibody CH2 reacts with Fc fragment of human IgM.
Clone number:
CH2
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: Recommended dilution: 1-4 ?g/ml. Extracellular and intracellular staining.
Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Fusion protein of human IgG Fc fragment.
Applications:
WB,ICC,ELISA,FC
Additional Info:
The mouse monoclonal antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Clone number:
EM-07
Antibody Isotype:
IgG1
Application Details:
Western blotting and ELISA: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments. The antibody EM-07 does not crossreact with IgM.
HQ-O Ready-To-Dilute (RTD TM ) Stain Reagent is designed to label amyloid plaques in paraffin-embedded or freshly cut frozen tissue sections. As a fluorescent zinc chelator, HQ-O is unique as it takes advantage of the known presence of concentrated zinc in amyloid plaques. Studies with HQ-O revealed that fluorescent plaque-like structures are only seen when synthetic A?x-42 is aggregated in the presence of zinc. Under blue light excitation, plaque structures appear bright green fluorescent in the brain parenchyma, correlating closely with plaque structures observed following A? antibody staining. HQ-O RTD TM staining reagent is compatible with other fluorophores, such as DAPI, Hoechst and ethidium bromide, as well as fluorescent-labelled antibodies with emission spectra in the blue and/or red emission range of fluorescent microscopes. Due to its zinc-chelating characteristics, HQ-O RTD TM staining reagent may visualize globular structures within blood vessels and intravascular leucocytes. HQ-O RTD TM staining reagent has multiple advantages over older blue-light exciting stains such as Thioflavin S. Thioflavin S typically exhibits relatively low contrast and resolution and suffers from bleed-through when excited by wavelengths other than blue light. HQ-O RTD TM staining reagent suffers none of these setbacks and not only provides a higher contrast and longer lasting dye, but because it lacks excitation bleed-through, HQ-O can be readily adapted to multiple labelling studies very easily. To visualize the HQ-O tracer, it is recommended to use a filter cube designed for visualizing Fluorescein/FITC or a blue-light laser. Although it can be seen with both narrow and wide-band pass filters, there is no need to use a narrow band filter since the compound does not bleed through when excited with other filters. A recommended excitation range of a wide band filter is 447-503 nm, with a peak at 475.
Background Info:
A novel zinc chelator, HQ-O, was developed for localizing zinc within amyloid plaques. The histology involves incubating tissue sections in a dilute aqueous solution of HQ-O.
Detection and fluorescent-staining of amyloid plaques in paraffin-embedded or freshly cut frozen tissue sections, please see detailed protocol for specific use instructions.
Alternative Names:
HQ-O tracer
Biosensis Brand:
Biosensis® RTD
Detection Method:
Fluorescence
Excitation/Emission:
To visualize the HQ-O tracer, it is recommended to use a filter cube designed for visualizing Fluorescein/FITC or a blue-light laser. Although it can be seen with both narrow and wide-band pass filters, there is no need to use a narrow band filter since the compound does not bleed through when excited with other filters. A recommended excitation range of a wide band filter is 447 503 nm, with a peak at 475 nm.
Shelf Life:
6 months after date of receipt (10X stock solution)
Use:
For research use only.
Kit Components:
One bottle containing 40 mL of 10X HQ-O RTD TM solution This quantity will be sufficient for approximately 8 Coplin Jars or 2-4 staining dishes.
Specificity:
As a fluorescent zinc chelator, HQ-O is unique as it takes advantage of the known presence of concentrated zinc in amyloid plaques. Studies with HQ-O revealed that fluorescent plaque-like structures are only seen when synthetic A?x-42 is aggregated in the presence of zinc. Under blue light excitation, plaque structures appear bright green fluorescent in the brain parenchyma, correlating closely with plaque structures observed following A? antibody staining.
Storage:
Store 10X stock solution at 2-8°C protected from light, for up to 6 months. The diluted dye (1X) should be used within 24 hours.
The Biosensis AG-400-AG kit utilizes an ethidium bromide counter stain for a quick and effective way to visualize cell nuclei and cell bodies of cells while under UV illumination allowing the assessment of amyloid plaques and cell/tissue positioning as well in one step. Amylo-Glo RTD Ready to Dilute Staining reagent is designed to stain amyloid plaques in tissue sections. This novel marker has several advantages over other conventional markers such as Thioflavin S and Congo Red because of its unique chemical and spectral properties. (L. Schmued et al. (2012) J.Neuroscience Methods 209:120- 126). Using Amylo-Glo results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. Its brightness makes it ideal for low magnification quantification studies, while its unique excitation/emission profile and mild staining conditions makes it ideal for combination for multiple immunofluorescent labeling studies. Amylo-Glo RTD is compatible with fresh, frozen, and formalin-fixed immunohistochemistry or cytochemistry, and it is particularly good for confocal and multiple labeling because of its high fluorescent intensity and high resistance to photo-bleaching. Moreover because Amylo-Glo fluoresces in the UV channel, double and triple labeling experiments can be performed very easily (see protocol).
Product Type:
Staining Reagent
Format:
The reagents in the Amyloid Plaque Stain Reagent (100x) are all supplied in a liquid format and are ready-to-dilute.
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Application Details:
Staining of amyloid plaques in human and animal tissues, see included protocol. EtBr counter stain stains nuclei and cell bodies for easy identification and spacial orientation.
Alternative Names:
AmyloGlo
Biosensis Brand:
Biosensis® RTD
Detection Method:
Fluorescence
Excitation/Emission:
Excitation Peak: 334 nm; Emission Peak: 533 nm - unbound, 438 nm when bound to amyloid. To visualize Amylo-glo in tissue, UV light is required. For example, Amylo-Glo tissue can be examined using an epifluoresent microscope with UV (Nikon UV-2A) filter cube. Excitation (325-375 nm) Emission (400-450 nm) is typical. Also note, it is not uncommon for Amylo-Glo to appear light yellow when examined by eye, yet appear a light blue color when photographed. <br>Visualization of EtBr: Ethidium bromide has an excitation peak of 300 nm and an emission peak 595 nm. Most UV compatible filter sets can be used.
Shelf Life:
6 months after date of receipt (unopened vial).
Use:
For research use only.
Kit Components:
1 bottle containing 40 mL of 10X Amylo-Glo RTD (A-G RTD) solution 1 bottle containing 40 mL of 10X A-G RTD Ethidium Bromide (EtBr RTD) solution
Product references:
Su IJ et al. (2021) "The Beneficial Effects of Combining Anti-A? Antibody NP106 and Curcumin Analog TML-6 on the Treatment of Alzheimer's Disease in APP/PS1 Mice." Int J Mol Sci. 23(1):556; Application: IHC/IF Species: Mouse Emre C et al. (2020) "Receptors for pro-resolving mediators are increased in Alzheimer's disease brain." Brain Pathol. [Epub ahead of print]; Application: IHC/IF Species: Human Hascup KN et al. (2019) "LY379268 Does Not Have Long-Term Procognitive Effects nor Attenuate Glutamatergic Signaling in A?PP/PS1 Mice." J Alzheimers Dis. [Epub ahead of print]; Application: IHC/IF Species: Mouse Hascup ER et al. (2018) "Diet-Induced Insulin Resistance Elevates Hippocampal Glutamate as well as VGLUT1 and GFAP Expression in A?PP/PS1 Mice." J Neurochem. [Epub ahead of print]; Application: IHC/IF Species: Mouse
Specificity:
Amyloid plaques both intraneuronal and vascular for A-G, Etbr, nuclei and cell bodies both DNA and RNA label
Storage:
The stock solution can be stored for up to 6 months at 2-8°C protected from light. No preservatives. Use sterile technique when handling and proper laboratory procedures.
Purification:
Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of two fluorescent isomers. No amount of starting material was detected.
Amylo-Glo RTD Ready to Dilute Staining reagent is designed to stain amyloid plaques in tissue sections. This novel marker has several advantages over other conventional markers such as Thioflavin S and Congo Red because of its unique chemical and spectral properties. (L. Schmued et al. (2012) J.Neuroscience Methods 209:120- 126). Using Amylo-Glo results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. Its brightness makes it ideal for low magnification quantification studies, while its unique excitation/emission profile and mild staining conditions makes it ideal for combination for multiple immunofluorescent labeling studies. Amylo-Glo RTD is compatible with fresh, frozen, and formalin-fixed immunohistochemistry or cytochemistry, and it is particularly good for confocal and multiple labeling because of its high fluorescent intensity and high resistance to photo-bleaching. Moreover because Amylo-Glo fluoresces in the UV channel, double and triple labeling experiments can be performed very easily (see protocol).
Product Type:
Staining Reagent
Format:
The reagents in the Amyloid Plaque Stain Reagent (100x) are all supplied in a liquid format and are ready-to-dilute.
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Application Details:
Staining of amyloid plaques in human and animal tissues, see included protocol
Alternative Names:
AmyloGlo
Biosensis Brand:
Biosensis® RTD
Detection Method:
Fluorescence
Excitation/Emission:
Excitation Peak: 334 nm; Emission Peak: 533 nm - unbound, 438 nm when bound to amyloid. To visualize Amylo-glo in tissue, UV light is required. For example, Amylo-Glo tissue can be examined using an epifluoresent microscope with UV (Nikon UV-2A) filter cube. Excitation (325-375 nm) Emission (400-450 nm) is typical. Also note, it is not uncommon for Amylo-Glo to appear light yellow when examined by eye, yet appear a light blue color when photographed.
Shelf Life:
6 months after date of receipt (unopened vial).
Use:
For research use only.
Kit Components:
5 mL of 100X Amylo-Glo RTD (A-G RTD) solution
Product references:
Silvin A et al. (2022) "Dual ontogeny of disease-associated microglia and disease inflammatory macrophages in aging and neurodegeneration" Immunity. [Epub ahead of print]; Application: IHC/IF Species: Mouse Shrader JM et al. (2022) "Distinct Brain Proteomic Signatures in Cerebral Small Vessel Disease Rat Models of Hypertension and Cerebral Amyloid Angiopathy" J Neuropathol Exp Neurol. [Epub ahead of print]; Application: IHC/IF Species: Rat Zagorski K et al. (2022) "Immunogenicity of MultiTEP-Platform-Based Recombinant Protein Vaccine, PV-1950R, Targeting Three B-Cell Antigenic Determinants of Pathological ?-Synuclein" Int J Mol Sci. [Epub ahead of print]; Application: IHC/IF Species: Mouse Shabestari SK et al. (2022) "Absence of microglia promotes diverse pathologies and early lethality in Alzheimers disease mice" Cell Rep. 39(11):110961; Application: IHC/IF Species: Mouse Davis J et al. (2022) "rTg-D: A novel transgenic rat model of cerebral amyloid angiopathy Type-2." Cerebral Circulation - Cognition and Behavior [Epub ahead of print]; Application: IHC/IF Species: Rat Salvadores N et al. (2022) "A? oligomers trigger necroptosis-mediated neurodegeneration via microglia activation in Alzheimer's disease." Acta Neuropathol Commun. 10(1):31; Application: IHC/IF Species: Human Javonillo DI et al. (2022) "Systematic Phenotyping and Characterization of the 3xTg-AD Mouse Model of Alzheimer's Disease." Front Neurosci. 15:785276; Application: IHC/IF Species: Mouse Hohsfield LA et al. (2022) "MAC2 is a long-lasting marker of peripheral cell infiltrates into the mouse CNS after bone marrow transplantation and coronavirus infection." Glia. [Epub ahead of print]; Application: IHC/IF Species: Mouse Tsay HJ et al. (2021) "EK100 and Antrodin C Improve Brain Amyloid Pathology in APP/PS1 Transgenic Mice by Promoting Microglial and Perivascular Clearance Pathways." Int J Mol Sci. 22(19):10413; Application: IHC/IF Species: Mouse Henningfield CM et al. (2021) "Microglia-specific ApoE knock-out does not alter Alzheimer's disease plaque pathogenesis or gene expression." Glia. [Epub ahead of print]; Application: IHC/IF Species: Mouse Da Mesquita S et al. (2021) "Meningeal lymphatics affect microglia responses and anti-A? immunotherapy." Nature. 593(7858):255-260; Application: IHC/IF Species: Mouse Lauterborn JC et al. (2021) "Increased excitatory to inhibitory synaptic ratio in parietal cortex samples from individuals with Alzheimer's disease. Nat Commun. 12(1):2603; Application: IHC/IF Species: Human Kim JH et al. (2021) "Gamma subunit of complement component 8 is a neuroinflammation inhibitor." Brain. 144(2):528-552; Application: IHC/IF Species: Mouse Claes C et al. (2021) "Plaque-associated human microglia accumulate lipid droplets in a chimeric model of Alzheimer's disease." Mol Neurodegener. 16(1):50; Application: IHC/IF Species: Mouse Crapser JD. (2021) "Investigating microglial regulation of the extracellular matrix in health and neurodegenerative disease." PhD Thesis ; Application: IHC/IF Species: Human Baglietto-Vargas D et al. (2021) "Generation of a humanized A? expressing mouse demonstrating aspects of Alzheimer's disease-like pathology." Nature Communications. 2(1):2421; Application: IHC/IF Species: Mouse Mistrik M et al. (2021) "Microthermal-induced subcellular-targeted protein damage in cells on plasmonic nanosilver-modified surfaces evokes a two-phase HSP-p97/VCP response." Nature Communications. 12, Article Number 719; Application: ICC/IF Species: Human Lemoine L et al. (2020) "Regional binding of tau and amyloid PET tracers in Down syndrome autopsy brain tissue." Mol Neurodegener. 15(1):68; Application: IHC/IF Species: Human Hascup KN et al. (2020) "Riluzole attenuates glutamatergic tone and cognitive decline in A?PP/PS1 mice." J Neurochem. [Epub ahead of print]; Application: IHC/IF Species: Mouse Holloway OG et al. (2020) "Microglia Demonstrate Local Mixed Inflammation and a Defined Morphological Shift in an APP/PS1 Mouse Model. J Alzheimers Dis. 77(4):1765-81; Application: IHC/IF Species: Mouse McQuade A et al. (2020) "Gene expression and functional deficits underlie TREM2-knockout microglia responses in human models of Alzheimer s disease. Nat Commun. 11(1):5370; Application: IHC/IF Species: Mouse Hascup KN et al. (2020) "Hippocampal alterations in glutamatergic signaling during amyloid progression in A?PP/PS1 mice." Sci Rep. 10(1):14503; Application: IHC/IF Species: Mouse Crapser JD et al. (2020) "Microglia facilitate loss of perineuronal nets in the Alzheimer's disease brain." EBioMedicine. 58:102919; Application: IHC/IF Species: Mouse Abe Y et al. (2020) "Behavioral and electrophysiological evidence for a neuroprotective role of aquaporin-4 in the 5xFAD transgenic mice model." Acta Neuropathol Commun. 8(1):67; Application: IHC/IF Species: Mouse Zhu X et al. (2020) "Robust neuroinflammation and perivascular pathology in rTg-DI rats, a novel model of microvascular cerebral amyloid angiopathy." J Neuroinflammation. 17(1):78; Application: IHC/IF Species: Rat Majewski L et al. (2020) "Transgenic Mice Overexpressing Human STIM2 and ORAI1 in Neurons Exhibit Changes in Behavior and Calcium Homeostasis but Show No Signs of Neurodegeneration." Int J Mol Sci. 21(3); Application: IHC/IF Species: Mouse Davtyan H et al. (2019) "Testing a MultiTEP-based combination vaccine to reduce A? and tau pathology in Tau22/5xFAD bigenic mice." Alzheimers Res Ther. 11(1):107; Application: IHC/IF Species: Mouse Yeh SHH et al. (2019) "A high-sucrose diet aggravates Alzheimer's disease pathology, attenuates hypothalamic leptin signaling, and impairs food-anticipatory activity in APPswe/PS1dE9 mice." Neurbiol. Aging. [In press]; Application: IHC/IF Species: Mouse Bharani KL et al. (2019) "Serum Pro-Bdnf Levels Correlate With Phospho-Tau Staining In Alzheimer's Disease." Neurbiol. Aging. [In press]; Application: IHC/IF Species: Human Hovakimyan A et al. (2019) "A MultiTEP platform-based epitope vaccine targeting the phosphatase activating domain (PAD) of tau: therapeutic efficacy in PS19 mice." Sci Rep. 9(1):15455; Application: IHC/IF Species: Human Hasselmann J et al. (2019) "Development of a Chimeric Model to Study and Manipulate Human Microglia In Vivo." Neuron. [Epub ahead of print]; Application: IHC/IF Species: Mouse Spangenberg E et al. (2019) "Sustained microglial depletion with CSF1R inhibitor impairs parenchymal plaque development in an Alzheimer's disease model." Nat Commun. 10(1):3758 (Supplementary Figure 1); Application: IHC/IF Species: Human Eggers C et al. (2019) "Novel cannabis flavonoid, cannflavin A displays both a hormetic and neuroprotective profile against amyloid _-mediated neurotoxicity in PC12 cells: comparison with geranylated flavonoids, mimulone and diplacone." Biochem Pharmacol. [Epub ahead of print]; Application: IHC/IF Species: Rat Dominguez E (2019) "Microglial Contributions to Alzheimer's Disease Pathogenesis." PhD Thesis, UC Irvine. Application: IHC/IF Species: Mouse Jovic M et al. (2019) "Short-term fish oil supplementation applied in presymptomatic stage of Alzheimer's disease enhances microglial/macrophage barrier and prevents neuritic dystrophy in parietal cortex of 5xFAD mouse model." PLoS One. 14(5):e0216726; Application: IHC/IF Species: Mouse Collins MJ et al. (2019) "Age moderates the effects of traumatic brain injury on beta-amyloid plaque load in APP/PS1 mice." J Neurotrauma. [Epub ahead of print]; Application: IHC/IF Species: Mouse Shukla AK et al. (2018) "CD11a expression distinguishes infiltrating myeloid cells from plaque-associated microglia in Alzheimer's disease." Glia. [Epub ahead of print]; Application: IHC/IF Species: Mouse Feng X et al. (2018) "Quantitative proteomics reveals distinct composition of amyloid plaques in Alzheimer's disease." Alzheimers Dement. [In press]; Application: IHC/IF Species: Human, mouse Davis J et al. (2018) "A Novel Transgenic Rat Model of Robust Cerebral Microvascular Amyloid with Prominent Vasculopathy." Am J Pathol. [Epub ahead of print]; Application: IHC/IF Species: Rat Palombo F et al. (2017) "Detection of A? plaque-associated astrogliosis in Alzheimer's disease brain by spectroscopic imaging and immunohistochemistry." Analyst. [Epub ahead of print]; Application: IF Species: Mouse Abud EM (2017) "Generation of Human Microglia from Induced Pluripotent Stem Cells to Study Innate Immunity in Neurological Diseases." PhD Thesis. 2017; Application: IF Species: Mouse Abud EM et al. (2017) "iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases." Neuron. 2017; 49(2):278-93 Application: IF Species: Mouse Solomon IH et al. (2017) "Brain and liver pathology, amyloid deposition, and interferon responses among older HIV-positive patients in the late HAART era." BMC Infect Dis. 2017; 17(1):151 Application: IF Species: Human Xu F et al. (2016) "Cerebral vascular amyloid seeds drive amyloid _-protein fibril assembly with a distinct anti-parallel structure." Nat Commun. 2016; 7:13527. Application: IF Species: Mouse Katsouri L et al. (2016) "PPARγ-coactivator-1_ gene transfer reduces neuronal loss and amyloid-_ generation by reducing _-secretase in an Alzheimer's disease model ." Proc Natl Acad Sci USA. 2016; 113(43):12292-97. Application: IF Species: Mouse Esposito G et al. (2016) "Autologous transplantation of intestine-isolated glia cells improves neuropathology and restores cognitive deficits in _ amyloid-induced neurodegeneration." Sci Rep. 2016; 6: 22605. Application: IF Species: Rat Marsh SE et al. (2016) "The adaptive immune system restrains Alzheimer's disease pathogenesis by modulating microglial function." Proc Natl Sci USA. Feb 16. pii: 201525466. Application: IF Species: Hu Fibrillar amyloid visualization. Kim YH et al. (2015) "A 3D human neural cell culture system for modeling Alzheimer's disease." Nat Protoc. Jul;10(7):985-1006. Application: IF Species: Hu , Human neural stem-cell-derived three-dimensional (3D) culture system. Nijholt DA et al. (2015) "Pregnancy Zone Protein is Increased in the Alzheimer's Disease Brain and Associates with Senile Plaques." J Alzheimer's Disease. 46(1):227-38. Application: IF Species: Hu Kamphuis W et al. (2015) "GFAP and vimentin deficiency alters gene expression in astrocytes and microglia in wild-type mice and changes the transcriptional response of reactive glia in mouse model for Alzheimer's disease." Glia. Jun;63(6):1036-56. Application: IF Species: Mouse Choi SH et al. (2014) "A three-dimensional human neural cell culture model of Alzheimer's disease." Nature Oct 12. doi: 10.1038/nature1380. Application: IF Species: Hu , Human neural stem-cell-derived three-dimensional (3D) culture system. Niedowicz DM et al. (2014). "Obesity and diabetes cause cognitive dysfunction in the absence of accelerated beta-amyloid deposition in a novel murine model of mixed or vascular dementia." Acta Neuropathol Commun. 2014 Jun 10;2:64.
Specificity:
Amyloid plaques both intraneuronal and vascular
Storage:
The stock solution can be stored for up to 6 months after date of receipt at 2-8°C protected from light. No preservatives. Use sterile technique when handling and proper laboratory procedures.
Purification:
Thin layer chromatography using alumina plates and a solvent system of ethanol and water (3:1) revealed the presence of two fluorescent isomers. No amount of starting material was detected.
The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. Fluoro-Jade C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols. Note: This product is equivalent to discontinued product AG325 from Merck-Millipore.
Product Type:
Staining Reagent
Format:
Dry, Coffee brown to brick red powder; hygroscopic powder keep dessicated.
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Applications:
FC,ICC
Application Details:
Following our detailed protocol, Fluoro-Jade B labels degenerating neurons which are visualised with blue light excitation, while DAPI (not included) counter stains cell nuclei, visualised with ultra-violet illumination. The Fluoro-Jade C dye can be used on all kinds of preserved tissues, including fresh-frozen, paraformaldehyde or formalin fixed, and formalin fixed, paraffin-embedded tissues.
Alternative Names:
FJC, Fluoro-Jade
Biosensis Brand:
Biosensis®
Detection Method:
Fluorescence
Excitation/Emission:
FJC visualization is accomplished using blue light or a 488 nm Laser. <br>Excitation Peak: 495 nm<br>Emission Peak: 521 nm<br>Filter system for visualizing: Fluorescein/FITC
Shelf Life:
6 months after date of receipt (unopened vial).
Use:
For research use only.
Kit Components:
Materials Provided: 30 mg Fluoro-Jade C, dry powder Detailed protocol Equipment and Reagents Required: Distilled water ACS grade Ethanol (200 proof) for slide & solution preparation 1% sodium hydroxide in 80% ethanol (basic alcohol solution) 0.1% Acetic Acid solution (in water) 70% ethanol in distilled water 0.06% (KMnO4) potassium permanganate solution DAPI powder or 100X solution (working range is 0.5-5 µg/mL) Xylene liquid Staining dishes/Coplin jars Cover slips DPX mounting media or another permanent mounting medium. Non-polar media are preferred over aqueous mounting media such as glycerin/water to obtain high- contrast images (refer to Appendix B in the protocol for a comparative analysis). Traditional fluorescent mounting mediums are not recommended because of their high pH. Slide warmer Convection oven
Specificity:
Degenerating neurons, and neuronal degeneration. There is no specific staining in normal healthy brain. Note: Some researchers under some conditions report blood vessel staining with Fluoro Jade. This may be because Fluoro Jade is an analogue of eosin (which stains blood cells). In general, good perfusion and preparation of the tissue should help prevent blood vessel staining but it may not be possible to eliminate it entirely. In our experience it is generally possible to distinguish neuronal from blood vessels staining by eye.
Storage:
The powdered dye can be stored desiccated at room temperature in the dark. Storage in a desiccator is recommended as FJB is hydroscopic. The 0.01% stock solution will remain stable for 3 months when stored in a refrigerator, in the dark. The 0.0001-0.0004% working solution in 0.1% acetic acid should be used within 4 hours of preparation. Diluted FJB dye solutions are not stable and should not be stored. The other diluted solutions can be reused and stored for up to 48 hours if refrigerated and protected from light. Best results require freshly diluted solutions.
Purification:
Silica TLC (acetanitrile/water, 6/4) revealed the presence of 2 fluorescent spots, presumably corresponding to the mono and di sulphate homologues. The presence of precursors or free fluorescein was not detected.
The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. The fluorescent dye Fluoro-Jade B (FJB), like its more purified brother Fluoro-Jade C (FJC), is an anionic fluorescein derivative useful for the histological staining of neurons undergoing degeneration. Fluoro-Jade B differs from FJC in that it is a slightly less refined chemical formulation and thus it does not quite provide the same level of signal to noise or high resolution as FJC. Nonetheless FJB is still widely used and works very well as a marker of degenerating neurons and even glia (see Damjanac M et al., Brain Res. 2007;1128(1):40-9). FJB operates nearly identically in protocol to that of FJC, and Fluoro-Jade B is compatible with several other labeling procedures including immunofluorescent and fluorescent Nissl techniques. Fluoro-Jade B stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade B exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols. Note: This product is equivalent to discontinued product AG310 from Merck-Millipore.
Product Type:
Staining Reagent
Format:
Dry, Coffee brown to brick red powder; hygroscopic powder keep dessicated.
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Applications:
FC,ICC
Application Details:
Following our detailed protocol, Fluoro-Jade C labels degenerating neurons which are visualised with blue light excitation, while DAPI (not included) counter stains cell nuclei, visualised with ultra-violet illumination. The Fluoro-Jade C dye can be used on all kinds of preserved tissues, including fresh-frozen, paraformaldehyde or formalin fixed, and formalin fixed, paraffin-embedded tissues.
Alternative Names:
FJB, Fluoro-Jade
Biosensis Brand:
Biosensis®
Detection Method:
Fluorescence
Excitation/Emission:
FJB visualization is accomplished using blue light or a 488 nm Laser. <br>Excitation Peak: 495 nm<br>Emission Peak: 521 nm<br>Filter system for visualizing: Fluorescein/FITC
Shelf Life:
6 months after date of receipt (unopened vial).
Use:
For research use only.
Kit Components:
Materials Provided: 30 mg Fluoro-Jade B, dry powder Detailed protocol Equipment and Reagents Required: Distilled water ACS grade Ethanol (200 proof) for slide & solution preparation 1% sodium hydroxide in 80% ethanol (basic alcohol solution) 0.1% Acetic Acid solution (in water) 70% ethanol in distilled water 0.06% (KMnO4) potassium permanganate solution DAPI powder or 100X solution (working range is 0.5-5 µg/mL) Xylene liquid Staining dishes/Coplin jars Cover slips DPX mounting media or another permanent mounting medium. Non-polar media are preferred over aqueous mounting media such as glycerin/water to obtain high- contrast images (refer to Appendix B in the protocol for a comparative analysis). Traditional fluorescent mounting mediums are not recommended because of their high pH. Slide warmer Convection oven
Product references:
Ikeda A et al. (2022) "Alteration of the neuronal and glial cell profiles in Neu1-deficient zebrafish" Glycoconj ; [Epub ahead of print]; Application: ICC/FC Species: Zebrafish
Choi I et al. (2022) "Interleukin-17A Mediates Hippocampal Damage and Aberrant Neurogenesis Contributing to Epilepsy-Associated Anxiety" Front. Mol. Neurosci. ; [Epub ahead of print]; Application: ICC/FC Species: Mouse
Cui Y et al. (2022) "Modified Citrus Pectin Alleviates Cerebral Ischemia/Reperfusion Injury by Inhibiting NLRP Inflammasome Activation via TLR4/NF-?B Signaling Pathway in Microglia" J Inflamm. Res. ; 15: 3369-3385; Application: ICC/FC Species: Mouse
Specificity:
Degenerating neurons, and neuronal degeneration. There is no specific staining in normal healthy brain. Note: Some researchers under some conditions report blood vessel staining with Fluoro Jade. This may be because Fluoro Jade is an analogue of eosin (which stains blood cells). In general, good perfusion and preparation of the tissue should help prevent blood vessel staining but it may not be possible to eliminate it entirely. In our experience it is generally possible to distinguish neuronal from blood vessels staining by eye.
Storage:
The powdered dye can be stored desiccated at room temperature in the dark. Storage in a desiccator is recommended as FJB is hydroscopic. The 0.01% stock solution will remain stable for 3 months when stored in a refrigerator, in the dark. The 0.0001-0.0004% working solution in 0.1% acetic acid should be used within 4 hours of preparation. Diluted FJB dye solutions are not stable and should not be stored. The other diluted solutions can be reused and stored for up to 48 hours if refrigerated and protected from light. Best results require freshly diluted solutions.
Purification:
Thin layer chromatograpy using cellulose plates and a solvent system of n-propinol, water, and ammonium hydroxide (6:5:2) revealed the presence of two fluorescent isomers and two trace non-fluorescent bands. No amount of fluorescein or Fluoro-Jade was present.
The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. Fluoro-Jade C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.
Product Type:
Staining Reagent
Format:
The reagents in the Fluoro Jade kit (10X) are all supplied in a liquid format and are ready-to-dilute.
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Applications:
FC,ICC,IHC-Frozen,IHC-Paraffin-embedded
Application Details:
The Fluoro-Jade C 'Ready to Dilute' (RTD) Staining Kit provides an easy to use assortment of Fluoro-Jade C, DAPI, sodium hydroxide and potassium permanganate in liquid form. Following our detailed protocol, Fluoro-Jade C labelled degenerating neurons are visualised with blue light excitation while DAPI counter stained cell nuclei are visualised with ultra-violet illumination. The Fluoro-Jade C Staining Kit can be used on all kinds of preserved tissues, including fresh-frozen, paraformaldehyde or formalin fixed, and formalin fixed, paraffin-embedded tissues.
Alternative Names:
FJC
Biosensis Brand:
Biosensis® RTD
Detection Method:
Fluorescence
Excitation/Emission:
FJC visualization is accomplished using blue light or a 488 nm Laser. Excitation Peak: 495 nm Emission Peak: 521 nm Filter system for visualizing: Fluorescein/FITC
Shelf Life:
Unopened kit 6 months at 2-8ºC protected from light. See Storage instructions for working solutions recommendations.
Use:
For research use only.
Kit Components:
Materials provided: Sodium Hydroxide, Solution A (Dilute 1:10 prior to use) - 20 mL Potassium Permanganate, Solution B (Dilute 1:10 prior to use) - 20 mL Fluoro-Jade C, Solution C (Dilute 1:10 prior to use) - 20 mL DAPI, Solution D (Add to diluted Fluoro-Jade C) - 20 mL Equipment and Reagents required: Gelatin coated microscope slides Staining dishes/Coplin jars Cover slips DPX mounting media Slide warmer Convection oven Distilled water Ethanol Xylene Number of slides processed: The actual number of slides processed by this kit will depend largely upon the vessel that is used to incubate the slides. If using a standard Coplin Jar, its capacity is 50 mL and typically holds 5 slides per jar. If using such a device, then 80-100 slides stained per 50 ml of working solution (or, 5 ml of stock solution) could be processed in one day. Note the diluted dye is NOT stable and will not store overnight. It is best to use freshly diluted dye each time an experimental batch is started. Final working concentrations of FJC: 0.0001% Final working concentration of KMnO4: 0.06%
Product references:
Total Number of References: 153 Latest Publications (2020-2022):
Li L et al. (2022) "A selective degeneration of cholinergic neurons mediated by NRADD in an Alzheimer's disease mouse model" Cell Insight. [Epub ahead of print] . Application: IF. Species: Mouse. Karino K et al. (2022) "Inhibitor of nuclear factor kappa-B kinase epsilon contributes to neuropsychiatric manifestations in lupus-prone mice through microglial activation" Arthritis Rheumatol. [Epub ahead of print] . Application: IF. Species: Mouse. Chen J et al. (2022) "Flufenamic acid improves survival and neurologic outcome after successful cardiopulmonary resuscitation in mice" J Neuroinflammation. 19(1):214 . Application: IF. Species: Mouse. Zou Y et al. (2022) "Learning and memory impairment and transcriptomic profile in hippocampus of offspring after maternal fructose exposure during gestation and lactation" Food Chem Toxicol. [Epub ahead of print] . Application: IF. Species: Rat. Jin P et al. (2022) "Aprepitant attenuates NLRC4-dependent neuronal pyroptosis via NK1R/PKC? pathway in a mouse model of intracerebral hemorrhage" J Neuroinflammation. 19(10):198 . Application: IF. Species: Mouse. Koike-Kumagai M et al. (2022) "Sirolimus relieves seizures and neuropsychiatric symptoms via changes of microglial polarity in tuberous sclerosis complex model mice" Neuropharmacology. [Epub ahead of print] . Application: IF. Species: Mouse. Wang D et al. (2022) "Mesenchymal stromal cell treatment attenuates repetitive mild traumatic brain injury-induced persistent cognitive deficits via suppressing ferroptosis" Neuroinflammation. 19(1):185 . Application: IF. Species: Mouse. Seo Y et al. (2022) "Mesenchymal stem cells target microglia via galectin-1 production to rescue aged mice from olfactory dysfunction" Biomed Pharmacother. 153:113347 . Application: IF. Species: Mouse. Duan R et al. (2022) "Recurrent de novo single point variant on the gene encoding Na+/K+ pump results in epilepsy" Prog Neurobiol. 216:102310 . Application: IF. Species: Mouse. Liu P et al. (2022) "Suppression of phosphodiesterase IV enzyme by Roflumilast ameliorates cognitive dysfunction in aged rats after sevoflurane anesthesia via PKA-CREB and MEK/ERK pathways" Eur J Neurosci. [Epub ahead of print] . Application: IF. Species: Rat. Boucher ML et al. (2022) "Titrating the Translational Relevance of a Low-Level Repetitive Head Impact Model" Front Neurol. 13:857654 . Application: IF. Species: Mouse. Qian L et al. (2022) "Interleukin-35 attenuates blood-brain barrier dysfunction caused by cerebral ischemia-reperfusion injury through inhibiting brain endothelial cell injury" Ann Transl Med. 10.21037 . Application: IF. Species: Mouse. Buentello DC et al. (2022) "Use of standard U-bottom and V-bottom well plates to generate neuroepithelial embryoid bodies" PLoS One. 17(5): e0262062 . Application: IF. Species: Human. Jin P et al. (2022) "Activation of LRP6 with HLY78 Attenuates Oxidative Stress and Neuronal Apoptosis via GSK3?/Sirt1/PGC-1? Pathway after ICH" Oxid Med Cell Longev. 2022: 7542468 . Application: IF. Species: Mouse. Wang J et al. (2022) "Irisin protects against sepsis-associated encephalopathy by suppressing ferroptosis via activation of the Nrf2/GPX4 signal axis" Free Radic Biol Med. [Epub ahead of print] . Application: IF. Species: Mouse. Tang T et al. (2022) "Ginkgetin Promotes M2 Polarization of Microglia and Exert Neuroprotection in Ischemic Stroke via Modulation of PPAR? Pathway" Neurochem Res. [Epub ahead of print] . Application: IF. Species: Rat. Zhang Z et al. (2022) "Inhibiting Microglia-Derived NLRP3 Alleviates Subependymal Edema and Cognitive Dysfunction in Posthemorrhagic Hydrocephalus after Intracerebral Hemorrhage via AMPK/Beclin-1 Pathway" Oxid. Med. Cell. Longev. [Epub ahead of print] . Application: IF. Species: Rat. Ren R et al. (2022) "Kynurenine/Aryl Hydrocarbon Receptor Modulates Mitochondria-Mediated Oxidative Stress and Neuronal Apoptosis in Experimental Intracerebral Hemorrhage" Antioxid Redox Signal. [Epub ahead of print] . Application: IF. Species: Mouse. Deforzh E et al. (2022) "Promoter and enhancer RNAs regulate chromatin reorganization and activation of miR-10b/HOXD locus, and neoplastic transformation in glioma" Mol Cell. [Epub ahead of print] . Application: IF. Species: Human, Mouse. Chen X et al. (2022) "Mechanism of Baicalein in Brain Injury After Intracerebral Hemorrhage by Inhibiting the ROS/NLRP3 Inflammasome Pathway" Inflammation. 45(2):590-602 . Application: IF. Species: Rat. Komatsu A et al. (2022) "Ammonia induces amyloidogenesis in astrocytes by promoting amyloid precursor protein translocation into the endoplasmic reticulum" J Bio; Chem. [Epub ahead of print] . Application: IF. Species: Mouse. Jin P et al. (2022) "Activation of LRP6 with HLY78 Attenuates Oxidative Stress and Neuronal Apoptosis via GSK3 ?/Sirt1/PGC-1 ? Pathway after ICH" Oxid Med Cell Longev. 7542468 . Application: IF. Species: Mouse. Hong Y et al. (2022) "Ultrasound stimulation improves inflammatory resolution, neuroprotection, and functional recovery after spinal cord injury" Sci Rep. 12(1):3636 . Application: IF. Species: Rat. Li J et al. (2022) "Inhibition of LRRK2-Rab10 Pathway Improves Secondary Brain Injury After Surgical Brain Injury in Rats" Front Surg. 8:749310 . Application: IF. Species: Rat. Yamashima T et al. (2022) "Hydroxynonenal Causes Lysosomal and Autophagic Failure in the Monkey." J Alzheimers Dis Parkinsonism. 12:529 . Application: IF. Species: Monkey. Salvadores N et al. (2022) "A? oligomers trigger necroptosis-mediated neurodegeneration via microglia activation in Alzheimer's disease." Acta Neuropathol Commun. 10(1):31 . Application: IF. Species: Mouse. Shi M et al. (2022) "Downregulation of TREM2/NF-?B signaling may damage the blood-brain barrier and aggravate neuronal apoptosis in experimental rats with surgically injured brain." Brain Res Bull. [Epub ahead of print] . Application: IF. Species: Rat. Zhao Y et al. (2022) "ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimers disease models." Nat Commun. 13(1):1121 . Application: IF. Species: Mouse. Fan X et al. (2022) "Inhibiting Sphingosine 1-Phosphate Receptor Subtype 3 Attenuates Brain Damage During Ischemia-Reperfusion Injury by Regulating nNOS/NO and Oxidative Stress." Front Neurosci. 16:838621 . Application: IF. Species: Mouse. Yu S et al. (2022) "BMS-470539 Attenuates Oxidative Stress and Neuronal Apoptosis via MC1R/cAMP/PKA/Nurr1 Signaling Pathway in a Neonatal Hypoxic-Ischemic Rat Model." Oxid Med Cell Longev. 2022:4054938 . Application: IF. Species: Rat. Kim EC et al. (2021) "Spontaneous seizure and memory loss in mice expressing an epileptic encephalopathy variant in the calmodulin-binding domain of Kv7.2." Proc Natl Acad Sci USA. 118(51):e2021265118 . Application: IF. Species: Mouse. Zhang Y et al. (2021) "GSK-3? inhibition elicits a neuroprotection by restoring lysosomal dysfunction in neurons via facilitation of TFEB nuclear translocation after ischemic stroke." Brain Res. [Epub ahead of print] . Application: IF. Species: Rat. Kondoh D et al. (2021) "Cotton rats (Sigmodon hispidus) with a high prevalence of hydrocephalus without clinical symptoms." Neuropathology. [Epub ahead of print] . Application: IF. Species: Rat. Iwasa K et al. (2021) "A peripheral lipid sensor GPR120 remotely contributes to suppression of PGD2-microglia-provoked neuroinflammation and neurodegeneration in the mouse hippocampus." J Neuroinflammation. 18(1):304 . Application: IF. Species: Mouse. Rodriguez-Masso SR et al. (2021) "The Bradykinin B2 Receptor Agonist (NG291) Causes Rapid Onset of Transient BloodBrain Barrier Disruption Without Evidence of Early Brain Injury." Front Neurosci. 15:791709 . Application: IF. Species: Rat. Li N et al. (2021) "Postcooling But Not Precooling Benefits Motor Recovery by Suppressing Cell Death after Surgical Spinal Cord Injury in Rats." World Neurosurg. [Epub ahead of print] . Application: IF. Species: Rat. Estrada H et al. (2021) "High-resolution fluorescence-guided transcranial ultrasound mapping in the live mouse brain. Sci Adv. 7(50):eabi5464 . Application: IF. Species: Mouse. Gu R et al. (2021) "Rh-CXCL-12 Attenuates Neuronal Pyroptosis after Subarachnoid Hemorrhage in Rats via Regulating the CXCR4/NLRP1 Pathway." Oxid Med Cell Longev. 2021:6966394 . Application: IF. Species: Rat. Huang L et al. (2021) "Docosahexaenoic acid reduces hypoglycemia-induced neuronal necroptosis via the peroxisome proliferator-activated receptor ?/nuclear factor-?B pathway." Brain Res. [Epub ahead of print] . Application: IF. Species: Mouse. Yaguchi A et al. (2021) "Efficient protein incorporation and release by a jigsaw-shaped self-assembling peptide hydrogel for injured brain regeneration." Nat Commun. 12(1):6623 . Application: IF. Species: Mouse. Liu Y et al. (2021) "Neuroprotection of minocycline by inhibition of extracellular matrix metalloproteinase inducer expression following intracerebral hemorrhage in mice." Neurosci Lett. [Epub ahead of print] . Application: IF. Species: Mouse. Lam V et al. (2021) "Synthesis of human amyloid restricted to liver results in an Alzheimer disease-like neurodegenerative phenotype." PLoS Biol. 19(9):e3001358 . Application: IF. Species: Mouse. Wu Z et al. (2021) "Melibiose Confers a Neuroprotection against Cerebral Ischemia/Reperfusion Injury by Ameliorating Autophagy Flux via Facilitation of TFEB Nuclear Translocation in Neurons." Life. 11(9), 948 . Application: IF. Species: Rat. Fang Y et al. (2021) "Pituitary adenylate cyclase-activating polypeptide attenuates mitochondria-mediated oxidative stress and neuronal apoptosis after subarachnoid hemorrhage in rats." Free Radic Biol Med. 174:236-248 . Application: IF. Species: Rat. Huan PS et al. (2021) "3,6'-Dithiopomalidomide Ameliorates Hippocampal Neurodegeneration, Microgliosis and Astrogliosis and Improves Cognitive Behaviors in Rats with a Moderate Traumatic Brain Injury." Int J Mol Sci. 22(15):8276 . Application: IF. Species: Mouse. Han M et al. (2021) "Localized Modification of Water Molecule Transport After Focused Ultrasound-Induced BloodBrain Barrier Disruption in Rat Brain." Front Neurosci. 15:685977 . Application: IF. Species: Rat. Gong Y et al. (2021) "Inhibition of the p?SPAK/p?NKCC1 signaling pathway protects the blood?brain barrier and reduces neuronal apoptosis in a rat model of surgical brain injury." Mol Med Rep. 24(4):717 . Application: IF. Species: Rat. Zhu L et al. (2021) "Neuroprotective effects of salidroside on ageing hippocampal neurons and naturally ageing mice via the PI3K/Akt/TERT pathway." Phytother Res. [Epub ahead of print] . Application: IF. Species: Mouse. Huang Y et al. (2021) "Kisspeptin-54 attenuates oxidative stress and neuronal apoptosis in early brain injury after subarachnoid hemorrhage in rats via GPR54/ARRB2/AKT/GSK3? signaling pathway." Free Radic Biol Med. 171:99-111 . Application: IF. Species: Rat. Gong Y et al. (2021) "Inhibition of the NKCC1/NF-?B Signaling Pathway Decreases Inflammation and Improves Brain Edema and Nerve Cell Apoptosis in an SBI Rat Model." Front Mol Neurosci. 14:641993 . Application: IF. Species: Rat. Caron NS et al. (2021) "Mutant Huntingtin Is Cleared from the Brain via Active Mechanisms in Huntington Disease." J Neurosci. 41(4):780-796 . Application: IF. Species: Human. Liu L et al. (2021) "A Novel Netrin-1-Derived Peptide Enhances Protection against Neuronal Death and Mitigates of Intracerebral Hemorrhage in Mice." Int J Mol Sci. 22(9):4829 . Application: IF. Species: Mouse. Zalewska K et al. (2021) "Corticosterone Administration Alters White Matter Tract Structure and Reduces Gliosis in the Sub-Acute Phase of Experimental Stroke." Int J Mol Sci. 22(13):6693 . Application: IF. Species: Mouse. Agrawal RR et al. (2021) "Neurometabolic Alterations After Traumatic Brain Injury: Links to Mitochondria-Associated ER Membranes and Alzheimers Disease." PhD Thesis . Application: IF. Species: Mouse. Zhou H et al. (2021) "AXL kinase-mediated astrocytic phagocytosis modulates outcomes of traumatic brain injury." J Neuroinflammation. 18(1):154 . Application: IF. Species: Mouse. Ousta A et al. (2021) "Microglial Activation and Neurological Outcomes in a Murine Model of Cardiac Arrest." Neurocrit Care. [Epub ahead of print] . Application: IF. Species: Mouse. Horinokita H et al. (2021) "Possible involvement of progranulin in the protective effect of elastase inhibitor on cerebral ischemic injuries of neuronal and glial cells." Mol Cell Neurosci. 113:103625 . Application: IF. Species: Human. Ho MH et al. (2021) "CCL5 via GPX1 activation protects hippocampal memory function after mild traumatic brain injury." Redox Biol. 46:102067 . Application: IF. Species: Mouse. Lian C et al. (2021) "Pentraxin 3 secreted by human adipose-derived stem cells promotes dopaminergic neuron repair in Parkinson's disease via the inhibition of apoptosis." FASEB J. 35(7):e21748 . Application: IF. Species: Mouse. Li Z et al. (2021) "The combination of deferoxamine and minocycline strengthens neuroprotective effect on acute intracerebral hemorrhage in rats." Neurol Res. [Epub ahead of print] . Application: IF. Species: Rat. Zhou K et al. (2021) "Dihydrolipoic acid enhances autophagy and alleviates neurological deficits after subarachnoid hemorrhage in rats." Exp Neurol. 342:113752 . Application: IF. Asuni GP et al. (2021) "Neuronal apoptosis induced by morphine withdrawal is mediated by the p75 neurotrophin receptor." J Neurochem. [Epub ahead of print] . Application: IF. Deng S et al. (2021) "Albumin Reduces Oxidative Stress and Neuronal Apoptosis via the ERK/Nrf2/HO-1 Pathway after Intracerebral Hemorrhage in Rats." Oxid. Med. Cell. Longev. 2021, Article ID 8891373 . Application: IF. Wu MY et al. (2021) "Possible mechanisms of the PERK pathway on neuronal apoptosis in a rat model of surgical brain injury." Am J Transl Res. 13(2):732-742 . Application: IF. Cai G et al. (2021) "Mesenchymal stem cell-derived exosome miR-542-3p suppresses inflammation and prevents cerebral infarction." Stem Cell Res Ther. 12(1)2 . Application: IF. Xu W et al. (2021) "Melanocortin 1 receptor attenuates early brain injury following subarachnoid hemorrhage by controlling mitochondrial metabolism via AMPK/SIRT1/PGC-1_ pathway in rats." Theranostics. 11(2):522-39 . Application: IF. Wu M et al. (2020) "The Blood Component Iron Causes Neuronal Apoptosis Following Intracerebral Hemorrhage via the PERK Pathway." Front. Neurol. 11:588548 . Application: IF. Wu D et al. (2020) "Activated WNK3 induced by intracerebral hemorrhage deteriorates brain injury maybe via WNK3/SPAK/NKCC1 pathway." Exp Neuro. 332:113386 . Application: IF. Fang Y et al. (2020) "HIF-1_ Mediates TRAIL-Induced Neuronal Apoptosis via Regulating DcR1 Expression Following Traumatic Brain Injury." Front Cell Neurosci. 14:192 . Application: IF. Lin CT et al. (2020) "3,6 -dithiopomalidomide reduces neural loss, inflammation, behavioral deficits in brain injury and microglial activation." Elife. 9:e54726 . Application: IF. Wu H et al. (2020) "Upregulated Nmnat2 causes neuronal death and increases seizure susceptibility in temporal lobe epilepsy." Brain Res Bull. [Epub ahead of print] . Application: IF. Zahedi K et al. (2020) "Ablation of polyamine catabolic enzymes provokes Purkinje cell damage, neuroinflammation, and severe ataxia." J Neuroinflammation. 17(1):301 . Application: IF. Hu X et al. (2020) "Rh-CSF1 Attenuates Oxidative Stress and Neuronal Apoptosis via the CSF1R/PLCG2/PKA/UCP2 Signaling Pathway in a Rat Model of Neonatal HIE." Oxid Med Cell Longev. 2020:6801587 . Application: IF. Hu H et al. (2020) "Transient receptor potential melastatin 2 contributes to neuroinflammation and negatively regulates cognitive outcomes in a pilocarpine-induced mouse model of epilepsy." Int Immunopharmacol. 87:106824 . Application: IF. Wang S et al. (2020) "Aging exacerbates impairments of cerebral blood flow autoregulation and cognition in diabetic rats." Geroscience. [Epub ahead of print] . Application: IF. Chen PY et al. (2020) "Stearic Acid Methyl Ester Affords Neuroprotection and Improves Functional Outcomes after Cardiac Arrest." Prostag Leukotr Ess. [In Press] . Application: IF. Gamdzyk M et al. (2020) "cGAS/STING Pathway Activation Contributes to Delayed Neurodegeneration in Neonatal Hypoxia-Ischemia Rat Model: Possible Involvement of LINE-1." Mol Neurobiol. 57(6): 2600-19 . Application: IF. Species: Rat Tang H et al. (2020) "Delayed Recanalization After MCAO Ameliorates Ischemic Stroke by Inhibiting Apoptosis via HGF/c-met/STAT3/Bcl-2 Pathway in Rats." Exp Neurol. [Epub ahead of print] . Application: IF. Species: Rat Ocak U et al. (2020) "Inhibition of mast cell tryptase attenuates neuroinflammation via PAR-2/p38/NF?B pathway following asphyxial cardiac arrest in rats." J Neuroinflammation. 17(1):144 . Application: IF. Species: Rat Huang J et al. (2020) "IRE1? inhibition attenuates neuronal pyroptosis via miR-125/NLRP1 pathway in a neonatal hypoxic-ischemic encephalopathy rat model." J Neuroinflammation. 17(1):152 . Application: IF. Species: Rat Fouda MA et al. (2020) "Estrogen-dependent hypersensitivity to diabetes-evoked cardiac autonomic dysregulation: Role of hypothalamic neuroinflammation." Life Sci. 2020 Mar 31:117598. [Epub ahead of print] . Application: IF. Species: Rat Zhou Z et al. (2020) "Sodium butyrate attenuated neuronal apoptosis via GPR41/G??/PI3K/Akt pathway after MCAO in rats." J Cerebr Blood F Met. [Epub ahead of print]. Application: IF. Species: Rat Enam SF et al. (2020) "Engineering Cytokine and Macrophage Enrichment at Sites of Injury." PhD Thesis . Application: IF. Species: Mouse
Specificity:
Degenerating neurons, and neuronal degeneration. There is no specific staining in normal healthy brain. Note: Some researchers under some conditions report blood vessel staining with Fluoro Jade. This may be because Fluoro Jade is an analogue of eosin (which stains blood cells). In general, good perfusion and preparation of the tissue should help prevent blood vessel staining but it may not be possible to eliminate it entirely. In our experience it is generally possible to distinguish neuronal from blood vessels staining by eye.
Storage:
The unopened kit can be stored for up to 6 months at 2-8ºC after the date of receipt. The kit and components should be stored protected from light. Diluted FJC dye solutions are not stable and should be used within 4 hours of making. The other diluted solutions can be reused and stored for up to 48 hours if refrigerated and protected from light. Best results require freshly diluted solutions. We recommend using aseptic techniques when handling the reagents to avoid bacterial growth and contamination.<br><br>The FJC Ready to dilute kit is shipped ambient and stable at room temperature during transport. Refrigerate upon arrival, do not freeze.
The causes and effects of neuronal degeneration are of major interest to a wide variety of neuroscientists. Paralleling this growing interest is an increasing number of methods applicable to the detection of neuronal degeneration. Fluoro-Jade C stains all degenerating neurons regardless of specific insult or mechanism of cell death. Fluoro-Jade C exhibits the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localising not only degenerating nerve cell bodies but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols.
Product Type:
Staining Reagent
Format:
The reagents in the Fluoro Jade kit (10X) are all supplied in a liquid format and are ready-to-dilute.
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Applications:
FC,ICC,IHC-Frozen,IHC-Paraffin-embedded
Application Details:
The Fluoro-Jade C 'Ready to Dilute' (RTD) Staining Kit provides an easy to use assortment of Fluoro-Jade C, DAPI, sodium hydroxide and potassium permanganate in liquid form. Following our detailed protocol, Fluoro-Jade C labelled degenerating neurons are visualised with blue light excitation while DAPI counter stained cell nuclei are visualised with ultra-violet illumination. The Fluoro-Jade C Staining Kit can be used on all kinds of preserved tissues, including fresh-frozen, paraformaldehyde or formalin fixed, and formalin fixed, paraffin-embedded tissues.
Alternative Names:
FJC
Biosensis Brand:
Biosensis® RTD
Detection Method:
Fluorescence
Excitation/Emission:
FJC visualization is accomplished using blue light or a 488 nm Laser. Excitation Peak: 495 nm Emission Peak: 521 nm Filter system for visualizing: Fluorescein/FITC
Shelf Life:
Unopened kit 6 months at 2-8ºC protected from light. See Storage instructions for working solutions recommendations.
Use:
For research use only.
Kit Components:
Materials provided: Sodium Hydroxide, Solution A (Dilute 1:10 prior to use) - 40 mL Potassium Permanganate, Solution B (Dilute 1:10 prior to use) - 40 mL Fluoro-Jade C, Solution C (Dilute 1:10 prior to use) - 40 mL DAPI, Solution D (Add to diluted Fluoro-Jade C) - 40 mL Equipment and Reagents required: Gelatin coated microscope slides Staining dishes/Coplin jars Cover slips DPX mounting media Slide warmer Convection oven Distilled water Ethanol Xylene Number of slides processed: The actual number of slides processed by this kit will depend largely upon the vessel that is used to incubate the slides. If using a standard Coplin Jar, its capacity is 50 mL and typically holds 5 slides per jar. If using such a device, then 80-100 slides stained per 50 ml of working solution (or, 5 ml of stock solution) could be processed in one day. Note the diluted dye is NOT stable and will not store overnight. It is best to use freshly diluted dye each time an experimental batch is started. Final working concentrations of FJC: 0.0001% Final working concentration of KMnO4: 0.06%
Product references:
Total Number of References: 153 Latest Publications (2020-2022):
Li L et al. (2022) "A selective degeneration of cholinergic neurons mediated by NRADD in an Alzheimer's disease mouse model" Cell Insight. [Epub ahead of print] . Application: IF. Species: Mouse. Karino K et al. (2022) "Inhibitor of nuclear factor kappa-B kinase epsilon contributes to neuropsychiatric manifestations in lupus-prone mice through microglial activation" Arthritis Rheumatol. [Epub ahead of print] . Application: IF. Species: Mouse. Chen J et al. (2022) "Flufenamic acid improves survival and neurologic outcome after successful cardiopulmonary resuscitation in mice" J Neuroinflammation. 19(1):214 . Application: IF. Species: Mouse. Zou Y et al. (2022) "Learning and memory impairment and transcriptomic profile in hippocampus of offspring after maternal fructose exposure during gestation and lactation" Food Chem Toxicol. [Epub ahead of print] . Application: IF. Species: Rat. Jin P et al. (2022) "Aprepitant attenuates NLRC4-dependent neuronal pyroptosis via NK1R/PKC? pathway in a mouse model of intracerebral hemorrhage" J Neuroinflammation. 19(10):198 . Application: IF. Species: Mouse. Koike-Kumagai M et al. (2022) "Sirolimus relieves seizures and neuropsychiatric symptoms via changes of microglial polarity in tuberous sclerosis complex model mice" Neuropharmacology. [Epub ahead of print] . Application: IF. Species: Mouse. Wang D et al. (2022) "Mesenchymal stromal cell treatment attenuates repetitive mild traumatic brain injury-induced persistent cognitive deficits via suppressing ferroptosis" Neuroinflammation. 19(1):185 . Application: IF. Species: Mouse. Seo Y et al. (2022) "Mesenchymal stem cells target microglia via galectin-1 production to rescue aged mice from olfactory dysfunction" Biomed Pharmacother. 153:113347 . Application: IF. Species: Mouse. Duan R et al. (2022) "Recurrent de novo single point variant on the gene encoding Na+/K+ pump results in epilepsy" Prog Neurobiol. 216:102310 . Application: IF. Species: Mouse. Liu P et al. (2022) "Suppression of phosphodiesterase IV enzyme by Roflumilast ameliorates cognitive dysfunction in aged rats after sevoflurane anesthesia via PKA-CREB and MEK/ERK pathways" Eur J Neurosci. [Epub ahead of print] . Application: IF. Species: Rat. Boucher ML et al. (2022) "Titrating the Translational Relevance of a Low-Level Repetitive Head Impact Model" Front Neurol. 13:857654 . Application: IF. Species: Mouse. Qian L et al. (2022) "Interleukin-35 attenuates blood-brain barrier dysfunction caused by cerebral ischemia-reperfusion injury through inhibiting brain endothelial cell injury" Ann Transl Med. 10.21037 . Application: IF. Species: Mouse. Buentello DC et al. (2022) "Use of standard U-bottom and V-bottom well plates to generate neuroepithelial embryoid bodies" PLoS One. 17(5): e0262062 . Application: IF. Species: Human. Jin P et al. (2022) "Activation of LRP6 with HLY78 Attenuates Oxidative Stress and Neuronal Apoptosis via GSK3?/Sirt1/PGC-1? Pathway after ICH" Oxid Med Cell Longev. 2022: 7542468 . Application: IF. Species: Mouse. Wang J et al. (2022) "Irisin protects against sepsis-associated encephalopathy by suppressing ferroptosis via activation of the Nrf2/GPX4 signal axis" Free Radic Biol Med. [Epub ahead of print] . Application: IF. Species: Mouse. Tang T et al. (2022) "Ginkgetin Promotes M2 Polarization of Microglia and Exert Neuroprotection in Ischemic Stroke via Modulation of PPAR? Pathway" Neurochem Res. [Epub ahead of print] . Application: IF. Species: Rat. Zhang Z et al. (2022) "Inhibiting Microglia-Derived NLRP3 Alleviates Subependymal Edema and Cognitive Dysfunction in Posthemorrhagic Hydrocephalus after Intracerebral Hemorrhage via AMPK/Beclin-1 Pathway" Oxid. Med. Cell. Longev. [Epub ahead of print] . Application: IF. Species: Rat. Ren R et al. (2022) "Kynurenine/Aryl Hydrocarbon Receptor Modulates Mitochondria-Mediated Oxidative Stress and Neuronal Apoptosis in Experimental Intracerebral Hemorrhage" Antioxid Redox Signal. [Epub ahead of print] . Application: IF. Species: Mouse. Deforzh E et al. (2022) "Promoter and enhancer RNAs regulate chromatin reorganization and activation of miR-10b/HOXD locus, and neoplastic transformation in glioma" Mol Cell. [Epub ahead of print] . Application: IF. Species: Human, Mouse. Chen X et al. (2022) "Mechanism of Baicalein in Brain Injury After Intracerebral Hemorrhage by Inhibiting the ROS/NLRP3 Inflammasome Pathway" Inflammation. 45(2):590-602 . Application: IF. Species: Rat. Komatsu A et al. (2022) "Ammonia induces amyloidogenesis in astrocytes by promoting amyloid precursor protein translocation into the endoplasmic reticulum" J Bio; Chem. [Epub ahead of print] . Application: IF. Species: Mouse. Jin P et al. (2022) "Activation of LRP6 with HLY78 Attenuates Oxidative Stress and Neuronal Apoptosis via GSK3 ?/Sirt1/PGC-1 ? Pathway after ICH" Oxid Med Cell Longev. 7542468 . Application: IF. Species: Mouse. Hong Y et al. (2022) "Ultrasound stimulation improves inflammatory resolution, neuroprotection, and functional recovery after spinal cord injury" Sci Rep. 12(1):3636 . Application: IF. Species: Rat. Li J et al. (2022) "Inhibition of LRRK2-Rab10 Pathway Improves Secondary Brain Injury After Surgical Brain Injury in Rats" Front Surg. 8:749310 . Application: IF. Species: Rat. Yamashima T et al. (2022) "Hydroxynonenal Causes Lysosomal and Autophagic Failure in the Monkey." J Alzheimers Dis Parkinsonism. 12:529 . Application: IF. Species: Monkey. Salvadores N et al. (2022) "A? oligomers trigger necroptosis-mediated neurodegeneration via microglia activation in Alzheimer's disease." Acta Neuropathol Commun. 10(1):31 . Application: IF. Species: Mouse. Shi M et al. (2022) "Downregulation of TREM2/NF-?B signaling may damage the blood-brain barrier and aggravate neuronal apoptosis in experimental rats with surgically injured brain." Brain Res Bull. [Epub ahead of print] . Application: IF. Species: Rat. Zhao Y et al. (2022) "ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimers disease models." Nat Commun. 13(1):1121 . Application: IF. Species: Mouse. Fan X et al. (2022) "Inhibiting Sphingosine 1-Phosphate Receptor Subtype 3 Attenuates Brain Damage During Ischemia-Reperfusion Injury by Regulating nNOS/NO and Oxidative Stress." Front Neurosci. 16:838621 . Application: IF. Species: Mouse. Yu S et al. (2022) "BMS-470539 Attenuates Oxidative Stress and Neuronal Apoptosis via MC1R/cAMP/PKA/Nurr1 Signaling Pathway in a Neonatal Hypoxic-Ischemic Rat Model." Oxid Med Cell Longev. 2022:4054938 . Application: IF. Species: Rat. Kim EC et al. (2021) "Spontaneous seizure and memory loss in mice expressing an epileptic encephalopathy variant in the calmodulin-binding domain of Kv7.2." Proc Natl Acad Sci USA. 118(51):e2021265118 . Application: IF. Species: Mouse. Zhang Y et al. (2021) "GSK-3? inhibition elicits a neuroprotection by restoring lysosomal dysfunction in neurons via facilitation of TFEB nuclear translocation after ischemic stroke." Brain Res. [Epub ahead of print] . Application: IF. Species: Rat. Kondoh D et al. (2021) "Cotton rats (Sigmodon hispidus) with a high prevalence of hydrocephalus without clinical symptoms." Neuropathology. [Epub ahead of print] . Application: IF. Species: Rat. Iwasa K et al. (2021) "A peripheral lipid sensor GPR120 remotely contributes to suppression of PGD2-microglia-provoked neuroinflammation and neurodegeneration in the mouse hippocampus." J Neuroinflammation. 18(1):304 . Application: IF. Species: Mouse. Rodriguez-Masso SR et al. (2021) "The Bradykinin B2 Receptor Agonist (NG291) Causes Rapid Onset of Transient BloodBrain Barrier Disruption Without Evidence of Early Brain Injury." Front Neurosci. 15:791709 . Application: IF. Species: Rat. Li N et al. (2021) "Postcooling But Not Precooling Benefits Motor Recovery by Suppressing Cell Death after Surgical Spinal Cord Injury in Rats." World Neurosurg. [Epub ahead of print] . Application: IF. Species: Rat. Estrada H et al. (2021) "High-resolution fluorescence-guided transcranial ultrasound mapping in the live mouse brain. Sci Adv. 7(50):eabi5464 . Application: IF. Species: Mouse. Gu R et al. (2021) "Rh-CXCL-12 Attenuates Neuronal Pyroptosis after Subarachnoid Hemorrhage in Rats via Regulating the CXCR4/NLRP1 Pathway." Oxid Med Cell Longev. 2021:6966394 . Application: IF. Species: Rat. Huang L et al. (2021) "Docosahexaenoic acid reduces hypoglycemia-induced neuronal necroptosis via the peroxisome proliferator-activated receptor ?/nuclear factor-?B pathway." Brain Res. [Epub ahead of print] . Application: IF. Species: Mouse. Yaguchi A et al. (2021) "Efficient protein incorporation and release by a jigsaw-shaped self-assembling peptide hydrogel for injured brain regeneration." Nat Commun. 12(1):6623 . Application: IF. Species: Mouse. Liu Y et al. (2021) "Neuroprotection of minocycline by inhibition of extracellular matrix metalloproteinase inducer expression following intracerebral hemorrhage in mice." Neurosci Lett. [Epub ahead of print] . Application: IF. Species: Mouse. Lam V et al. (2021) "Synthesis of human amyloid restricted to liver results in an Alzheimer disease-like neurodegenerative phenotype." PLoS Biol. 19(9):e3001358 . Application: IF. Species: Mouse. Wu Z et al. (2021) "Melibiose Confers a Neuroprotection against Cerebral Ischemia/Reperfusion Injury by Ameliorating Autophagy Flux via Facilitation of TFEB Nuclear Translocation in Neurons." Life. 11(9), 948 . Application: IF. Species: Rat. Fang Y et al. (2021) "Pituitary adenylate cyclase-activating polypeptide attenuates mitochondria-mediated oxidative stress and neuronal apoptosis after subarachnoid hemorrhage in rats." Free Radic Biol Med. 174:236-248 . Application: IF. Species: Rat. Huan PS et al. (2021) "3,6'-Dithiopomalidomide Ameliorates Hippocampal Neurodegeneration, Microgliosis and Astrogliosis and Improves Cognitive Behaviors in Rats with a Moderate Traumatic Brain Injury." Int J Mol Sci. 22(15):8276 . Application: IF. Species: Mouse. Han M et al. (2021) "Localized Modification of Water Molecule Transport After Focused Ultrasound-Induced BloodBrain Barrier Disruption in Rat Brain." Front Neurosci. 15:685977 . Application: IF. Species: Rat. Gong Y et al. (2021) "Inhibition of the p?SPAK/p?NKCC1 signaling pathway protects the blood?brain barrier and reduces neuronal apoptosis in a rat model of surgical brain injury." Mol Med Rep. 24(4):717 . Application: IF. Species: Rat. Zhu L et al. (2021) "Neuroprotective effects of salidroside on ageing hippocampal neurons and naturally ageing mice via the PI3K/Akt/TERT pathway." Phytother Res. [Epub ahead of print] . Application: IF. Species: Mouse. Huang Y et al. (2021) "Kisspeptin-54 attenuates oxidative stress and neuronal apoptosis in early brain injury after subarachnoid hemorrhage in rats via GPR54/ARRB2/AKT/GSK3? signaling pathway." Free Radic Biol Med. 171:99-111 . Application: IF. Species: Rat. Gong Y et al. (2021) "Inhibition of the NKCC1/NF-?B Signaling Pathway Decreases Inflammation and Improves Brain Edema and Nerve Cell Apoptosis in an SBI Rat Model." Front Mol Neurosci. 14:641993 . Application: IF. Species: Rat. Caron NS et al. (2021) "Mutant Huntingtin Is Cleared from the Brain via Active Mechanisms in Huntington Disease." J Neurosci. 41(4):780-796 . Application: IF. Species: Human. Liu L et al. (2021) "A Novel Netrin-1-Derived Peptide Enhances Protection against Neuronal Death and Mitigates of Intracerebral Hemorrhage in Mice." Int J Mol Sci. 22(9):4829 . Application: IF. Species: Mouse. Zalewska K et al. (2021) "Corticosterone Administration Alters White Matter Tract Structure and Reduces Gliosis in the Sub-Acute Phase of Experimental Stroke." Int J Mol Sci. 22(13):6693 . Application: IF. Species: Mouse. Agrawal RR et al. (2021) "Neurometabolic Alterations After Traumatic Brain Injury: Links to Mitochondria-Associated ER Membranes and Alzheimers Disease." PhD Thesis . Application: IF. Species: Mouse. Zhou H et al. (2021) "AXL kinase-mediated astrocytic phagocytosis modulates outcomes of traumatic brain injury." J Neuroinflammation. 18(1):154 . Application: IF. Species: Mouse. Ousta A et al. (2021) "Microglial Activation and Neurological Outcomes in a Murine Model of Cardiac Arrest." Neurocrit Care. [Epub ahead of print] . Application: IF. Species: Mouse. Horinokita H et al. (2021) "Possible involvement of progranulin in the protective effect of elastase inhibitor on cerebral ischemic injuries of neuronal and glial cells." Mol Cell Neurosci. 113:103625 . Application: IF. Species: Human. Ho MH et al. (2021) "CCL5 via GPX1 activation protects hippocampal memory function after mild traumatic brain injury." Redox Biol. 46:102067 . Application: IF. Species: Mouse. Lian C et al. (2021) "Pentraxin 3 secreted by human adipose-derived stem cells promotes dopaminergic neuron repair in Parkinson's disease via the inhibition of apoptosis." FASEB J. 35(7):e21748 . Application: IF. Species: Mouse. Li Z et al. (2021) "The combination of deferoxamine and minocycline strengthens neuroprotective effect on acute intracerebral hemorrhage in rats." Neurol Res. [Epub ahead of print] . Application: IF. Species: Rat. Zhou K et al. (2021) "Dihydrolipoic acid enhances autophagy and alleviates neurological deficits after subarachnoid hemorrhage in rats." Exp Neurol. 342:113752 . Application: IF. Asuni GP et al. (2021) "Neuronal apoptosis induced by morphine withdrawal is mediated by the p75 neurotrophin receptor." J Neurochem. [Epub ahead of print] . Application: IF. Deng S et al. (2021) "Albumin Reduces Oxidative Stress and Neuronal Apoptosis via the ERK/Nrf2/HO-1 Pathway after Intracerebral Hemorrhage in Rats." Oxid. Med. Cell. Longev. 2021, Article ID 8891373 . Application: IF. Wu MY et al. (2021) "Possible mechanisms of the PERK pathway on neuronal apoptosis in a rat model of surgical brain injury." Am J Transl Res. 13(2):732-742 . Application: IF. Cai G et al. (2021) "Mesenchymal stem cell-derived exosome miR-542-3p suppresses inflammation and prevents cerebral infarction." Stem Cell Res Ther. 12(1)2 . Application: IF. Xu W et al. (2021) "Melanocortin 1 receptor attenuates early brain injury following subarachnoid hemorrhage by controlling mitochondrial metabolism via AMPK/SIRT1/PGC-1_ pathway in rats." Theranostics. 11(2):522-39 . Application: IF. Wu M et al. (2020) "The Blood Component Iron Causes Neuronal Apoptosis Following Intracerebral Hemorrhage via the PERK Pathway." Front. Neurol. 11:588548 . Application: IF. Wu D et al. (2020) "Activated WNK3 induced by intracerebral hemorrhage deteriorates brain injury maybe via WNK3/SPAK/NKCC1 pathway." Exp Neuro. 332:113386 . Application: IF. Fang Y et al. (2020) "HIF-1_ Mediates TRAIL-Induced Neuronal Apoptosis via Regulating DcR1 Expression Following Traumatic Brain Injury." Front Cell Neurosci. 14:192 . Application: IF. Lin CT et al. (2020) "3,6 -dithiopomalidomide reduces neural loss, inflammation, behavioral deficits in brain injury and microglial activation." Elife. 9:e54726 . Application: IF. Wu H et al. (2020) "Upregulated Nmnat2 causes neuronal death and increases seizure susceptibility in temporal lobe epilepsy." Brain Res Bull. [Epub ahead of print] . Application: IF. Zahedi K et al. (2020) "Ablation of polyamine catabolic enzymes provokes Purkinje cell damage, neuroinflammation, and severe ataxia." J Neuroinflammation. 17(1):301 . Application: IF. Hu X et al. (2020) "Rh-CSF1 Attenuates Oxidative Stress and Neuronal Apoptosis via the CSF1R/PLCG2/PKA/UCP2 Signaling Pathway in a Rat Model of Neonatal HIE." Oxid Med Cell Longev. 2020:6801587 . Application: IF. Hu H et al. (2020) "Transient receptor potential melastatin 2 contributes to neuroinflammation and negatively regulates cognitive outcomes in a pilocarpine-induced mouse model of epilepsy." Int Immunopharmacol. 87:106824 . Application: IF. Wang S et al. (2020) "Aging exacerbates impairments of cerebral blood flow autoregulation and cognition in diabetic rats." Geroscience. [Epub ahead of print] . Application: IF. Chen PY et al. (2020) "Stearic Acid Methyl Ester Affords Neuroprotection and Improves Functional Outcomes after Cardiac Arrest." Prostag Leukotr Ess. [In Press] . Application: IF. Gamdzyk M et al. (2020) "cGAS/STING Pathway Activation Contributes to Delayed Neurodegeneration in Neonatal Hypoxia-Ischemia Rat Model: Possible Involvement of LINE-1." Mol Neurobiol. 57(6): 2600-19 . Application: IF. Species: Rat Tang H et al. (2020) "Delayed Recanalization After MCAO Ameliorates Ischemic Stroke by Inhibiting Apoptosis via HGF/c-met/STAT3/Bcl-2 Pathway in Rats." Exp Neurol. [Epub ahead of print] . Application: IF. Species: Rat Ocak U et al. (2020) "Inhibition of mast cell tryptase attenuates neuroinflammation via PAR-2/p38/NF?B pathway following asphyxial cardiac arrest in rats." J Neuroinflammation. 17(1):144 . Application: IF. Species: Rat Huang J et al. (2020) "IRE1? inhibition attenuates neuronal pyroptosis via miR-125/NLRP1 pathway in a neonatal hypoxic-ischemic encephalopathy rat model." J Neuroinflammation. 17(1):152 . Application: IF. Species: Rat Fouda MA et al. (2020) "Estrogen-dependent hypersensitivity to diabetes-evoked cardiac autonomic dysregulation: Role of hypothalamic neuroinflammation." Life Sci. 2020 Mar 31:117598. [Epub ahead of print] . Application: IF. Species: Rat Zhou Z et al. (2020) "Sodium butyrate attenuated neuronal apoptosis via GPR41/G??/PI3K/Akt pathway after MCAO in rats." J Cerebr Blood F Met. [Epub ahead of print]. Application: IF. Species: Rat Enam SF et al. (2020) "Engineering Cytokine and Macrophage Enrichment at Sites of Injury." PhD Thesis . Application: IF. Species: Mouse
Specificity:
Degenerating neurons, and neuronal degeneration. There is no specific staining in normal healthy brain. Note: Some researchers under some conditions report blood vessel staining with Fluoro Jade. This may be because Fluoro Jade is an analogue of eosin (which stains blood cells). In general, good perfusion and preparation of the tissue should help prevent blood vessel staining but it may not be possible to eliminate it entirely. In our experience it is generally possible to distinguish neuronal from blood vessels staining by eye.
Storage:
The unopened kit can be stored for up to 6 months at 2-8ºC after the date of receipt. The kit and components should be stored protected from light. Diluted FJC dye solutions are not stable and should be used within 4 hours of making. The other diluted solutions can be reused and stored for up to 48 hours if refrigerated and protected from light. Best results require freshly diluted solutions. We recommend using aseptic techniques when handling the reagents to avoid bacterial growth and contamination.<br><br>The FJC Ready to dilute kit is shipped ambient and stable at room temperature during transport. Refrigerate upon arrival, do not freeze.
Black-Gold II is a novel haloaurophosphate complex which localises myelin within the central nervous system. The Black Gold II Ready-to-Dilute (RTD) Staining Kit allows you to localise myelin, both individual fibres and tracts, along with the option of co-localising cell bodies via the Toluidine Blue counter stain. Black Gold II labelled myelinated fibres appear nearly black while the Toluidine Blue O labelled cellular Nissl bodies are blue under bright field illumination. Black Gold II can demonstrate and characterise specific myelin changes associated with exposure to diverse neurotoxicants including kainic acid, domoic acid, 3-nitropropionic acid, Fluoro-Gold and isoniazid. Black Gold II can also be combined with other histochemical markers including Nissl stains, retrogradely transported fluorescent tracers and fluorescent markers of neuronal degeneration. The advantages associated with the Black-Gold II include high resolution, high contrast, short histochemical processing time, versatility and consistent reproducibility.
Product Type:
Staining Reagent
Format:
The reagents in the Black Gold kit (10X) are all supplied in a liquid format and are ready-to-dilute.
Species Reactivity:
Mammals
Applications:
IHC-Frozen,IHC-non-Paraffin-embedded
Application Details:
Black Gold II is a high resolution myelin stain with amyloid plaque counter stain. Its use is tailored to studies using formalin or paraformaldehyde fixed, non-paraffin embedded, non-solvent processed brain tissue. It can be used with both thick and thin sections. For thick sections, gelatin coated slides or slides specially designed to bind tissues sections should be use to avoid section loss. Free-floating sections can be used as well but sections are easier to handle and transfer when mounted on slides. A suggested method for thick sections is provided as a guide: Either frozen or vibratome sections are cut at a thickness of 20-50 ?m and collected in 0.1 M neutral phosphate buffer. The sections are then typically mounted on 1% gel-coated slides and then air dried on a slide warmer (at 50°C) for at least an hour until throughly dried and adhered to the slide. The sections can be stained loose, although the sections are easier to handle when mounted on slides. The mounted sections were rehydrated in distilled water for 2 minutes before being processed in the staining solutions.
Alternative Names:
BlackGold, Black and Gold
Biosensis Brand:
Biosensis® RTD
Detection Method:
Colorimetric
Shelf Life:
Unopened kit 6 months at 2-8ºC protected from light. See Storage instructions for working solutions recommendations.
Use:
For research use only.
Kit Components:
Black-Gold II (Dilute 1:10 prior to use) - 10 mL Sodium Thiosulfate, fixative (Dilute 1:10 prior to use) - 10 mL Toluidine Blue O (Dilute 1:10 prior to use) - 10 mL Acetic Acid (Dilute 1:10 prior to use) - 10 mL
Product references:
Lee J et al. (2022) PRMT1 is required for the generation of MHC-associated microglia and remyelination in the central nervous system. Life Sci Alliance. [Epub ahead of print] Germundson DL & Nagamoto-Combs K. (2022) Potential Role of Intracranial Mast Cells in Neuroinflammation and Neuropathology Associated with Food Allergy. Cells. 11(4):738. Kihara Y et al. (2021) Ponesimod inhibits astrocyte-mediated neuroinflammation and protects against cingulum demyelination via S1P1-selective modulation. FASEB J. 36(2):e22132. Toomey LM et al. (2021) Cuprizone feed formulation influences the extent of demyelinating disease pathology. Sci Rep. 11(1):22594. Del Fiacco M et al. (2018) TRPV1-Like Immunoreactivity in the Human Locus K, a Distinct Subregion of the Cuneate Nucleus. Cells. 2018 Jul 8;7(7). pii: E72. Ying YL et al. (2014) Adult neural precursor cells from the subventricular zone contribute significantly to oligodendrocyte regeneration and remyelination. J Neurosci. 2014 Oct 15;34(42):14128-46
Specificity:
Black-Gold II is a novel haloaurophosphate complex which localises myelin within the central nervous system.
Storage:
The kit can be transported at room temperature. Once received, the kit canbe stored for up to 12 months at 2-8°C protected from light. Diluted solutionscan be stored up to one month at 2-8°C protected from light.
Normal sheep serum from non-immunized animal, Sheep Polyclonal Antibody
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Sheep
Species Reactivity:
Non-Reactive (Negative Control)
Immunogen:
None. Sheep serum is obtained from non-immunized animals.
Applications:
Negative Control
Antibody Isotype:
Mixed
Application Details:
Use as negative control for IgG-purified sheep primary antibodies and at matching concentration.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Uncharacterized serum control
Storage:
Store lyophilized sheep whole serum at -20°C to -80°C protected from moisture. After reconstitution, divide whole serum nto useful aliquots and keep aliquots at -20°C to -80°C for a higher stability. Working aliquots can be kept at 2-8°C for up to 1 month. Avoid repetitive freeze/thaw cycles.
Normal sheep IgG from non-immunized animal, Sheep Polyclonal Antibody
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4, without preservatives.
Host Animal:
Sheep
Species Reactivity:
Non-Reactive (Negative Control)
Immunogen:
None. Sheep IgG is obtained from non-immunized animals.
Applications:
Negative Control
Antibody Isotype:
IgG
Application Details:
Use as negative control for IgG-purified sheep primary antibodies and at matching concentration.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Uncharacterized serum control
Storage:
Store lyophilized sheep IgG at -20°C to -80°C protected from moisture. After reconstitution divide IgG into useful aliquots and keep aliquots at -20°C to -80°C for a higher stability. Working aliquots can be kept at 2-8°C for up to 1 month. Avoid repetitive freeze/thaw cycles.
Sheep anti-Catenin beta Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Catenin beta is an adherens junction protein and has a role in the regulation of cell adhesion and in signal transduction through the Wnt pathway. At least 2 isoforms are produced by alternative splicing.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, and 0.05 % sodium azide.
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide (C-SNQLAWFDTDL) corresponding to the amino acid region 771-781 of human Catenin beta coupled to carrier protein KLH.
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB) and Immunoprecipitation. A concentration of 1.0 µg/mL is recommended for WB. Human Catenin beta (isoform 1) has a predicted length of 781 residues and MW of 86 kDa. A concentration of 5 µg/500 µg of lysate is recommended for immunoprecipitation. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB against the antigen. This antibody was also validated for immunoprecipitation in rat liver lysate (data not shown). Human; mouse; rat;
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Catenin beta is an adherens junction protein and has a role in the regulation of cell adhesion and in signal transduction through the Wnt pathway. At least 2 isoforms are produced by alternative splicing.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, and 0.05 % sodium azide.
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide (C-QSYLDSGIHSGATTTAPSL) corresponding to the amino acid region 28-46 of human Catenin beta coupled to carrier protein KLH. This antibody was designed to detect the active form of Catenin beta . The antibody exhibits a strong preference for dephosphorylated beta catenin protein by western blot but the antibody may show cross reactivity with phosphorylated catenin beta under certain conditions.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB) and Immunocytochemistry. A concentration of 2.0 µg/mL is recommended for WB. Human Catenin beta (isoform 1) has a predicted length of 781 residues and MW of 86 kDa. It has been observed as a 92 kDa band in SDS-reducing gels. A concentration of 4.0 µg/mL is recommended for Immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rat;
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released. This antibody is raised in sheep to detect the prodomain of NGF not the mature peptide.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
The recombinant prodomain fragment of human nerve growth factor
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, Immunofluorescence, ELISA, Western Blot, biological neutralization of proNGF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
pro-brain nerve growth factor; proNGF; NGF;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Sugimoto J et al. (2021). Fabry disease-associated globotriaosylceramide induces mechanical allodynia via activation of signaling through proNGF p75NTR but not mature NGF TrkA. Eur. J. Pharmacol. 895. Application: Neutralising ( in-vivo ). Ryu JC et al. (2018). Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. [Epub ahead of print]. Application: Western Blotting.
Specificity:
The specificity of this antibody has been confirmed by WB. It does NOT crossreact with proBDNF, proNT-3 or mature NGF. Confirmed to react with purified human proNGF and crossreact with mouse and rat proNGF
Storage:
Maintain lyophilized antibody at 2-8°C for up to 12 months after date of receipt. After reconstitution keep undiluted aliquots at -20°C for up to 6 months for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Brain derived neurotrophic factor (BDNF) is synthesized as a precursor (proBDNF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proBDNF is synthesized in neurons and glia (eg., microglia), transported anterogradely and retrogradely and may be released in an activity dependent manner. This antibody is raised in sheep to detect the prodomain of BDNF and not the mature peptide.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
The recombinant prodomain fragment of human brain-derived neurotrophic factor
Applications:
ELISA,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
ELISA, Western Blot, biological neutralization of proBDNF, Immunocytochemistry/Immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Proform brain derived neurotrophic factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed to react with purified human proBDNF, crossreact with mouse and rat proBDNF Cross reactivity with other species than human, mouse and rat has not yet been tested
Storage:
After reconstitution keep aliquots at -20ºC for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Sheep anti-Alpha-synuclein Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded.
Background Info:
Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. Alpha synuclein has been associated with several neurodegenerative diseases. A point mutation in the gene coding for the alpha-synuclein protein was the first discovery linking this protein to a rare familial form of Parkinson's disease (PD). Subsequently, other mutations in the alpha-synuclein gene have been identified in familial PD. The aggregated proteinaceous inclusions called Lewy bodies found in PD and cortical Lewy body dementia (LBD) were discovered to be predominantly alpha-synuclein. Aberrant aggregation of alpha-synuclein has been detected in an increasing number of neurodegenerative diseases, collectively known as synucleopathies. Alpha-synuclein exists physiologically in both soluble and membrane-bound states, in unstructured and alpha-helical conformations, respectively. The physiological function of alpha-synuclein appears to require its translocation between these subcellular compartments and interconversion between the 2 conformations. Abnormal processing of alpha-synuclein is predicted to lead to pathological changes in its binding properties and function.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (PQEGILEDMPVDPC) of human alpha synuclein protein (aa: 108-120) conjugated to diphtheria toxoid has been used as the immunogen.
Applications:
IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
IHC. Recommended to be used at a concentration of 1 µg/mL for immunohistochemistry (Paraffin sections). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Immunihistochemical analysis of human and rat brain indicates a high level of specificity for this antiserum. Specificity was also confirmed by western blot. This antiserum is known to react with human and rat alpha synuclein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Purification:
Affinity purified and dialysed against PBS. Contains 0.02% sodium azide.
Sheep anti-Beta-synuclein Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Beta-synuclein is a non-amyloid component of senile plaques found in Alzheimer disease. It could act as a regulator of SNCA aggregation. It protects neurons from staurosporine and 6 hydroxy dopamine -stimulated capspase activation in a p53-dependent manner. It localises to the cytoplasm and it is predominantly expressed in the brain where it is most concentrated in presynaptic nerve terminals. This protein is phosphorylated. This protein is also associated with the disease Brain iron accumulation type 1 (NBIA1).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Affinity purified and dialysed against PBS. Contains 0.02% sodium azide.
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (IEPLMEPEGSYEDPPQE) of human beta synuclein protein (aa: 108-125) conjugated to diptheria toxid has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
IgG
Application Details:
IHC. A working concentration of 1 µg/mL is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
SNCB
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Known to be specific for beta synuclein. This antiserum is known to react with beta synuclein of human, rat and other rodents.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. Alpha synuclein has been associated with several neurodegenerative diseases. A point mutation in the gene coding for the alpha-synuclein protein was the first discovery linking this protein to a rare familial form of Parkinson's disease (PD). Subsequently, other mutations in the alpha-synuclein gene have been identified in familial PD. The aggregated proteinaceous inclusions called Lewy bodies found in PD and cortical Lewy body dementia (LBD) were discovered to be predominantly alpha-synuclein. Aberrant aggregation of alpha-synuclein has been detected in an increasing number of neurodegenerative diseases, collectively known as synucleopathies. Alpha-synuclein exists physiologically in both soluble and membrane-bound states, in unstructured and alpha-helical conformations, respectively. The physiological function of alpha-synuclein appears to require its translocation between these subcellular compartments and interconversion between the 2 conformations. Abnormal processing of alpha-synuclein is predicted to lead to pathological changes in its binding properties and function.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide (CMPVDPDNEAYEMPSEE) of human alpha synuclein protein (aa: 116-131) conjugated to diphtheria toxoid has been used as the immunogen.
Applications:
ELISA,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgG
Application Details:
Immunohistochemistry (IHC): 1-4 µg/mL (paraffin sections)<br>Western Blotting (WB): 0.5 - 2.0 µg/mL. Fixing of proteins on membrane with 0.4% formaldehyde (30 min at room temperature) recommended, see Lee & Kamitani, 2011.<br>Flow Cytometry: 2 µg antibody per ~10^6 cells, methanol-fixed.<br>Immunocytochemistry (ICC): 1-4 µg/mL, 4% formaldehyde-fixed cells.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Kuo Y.M. et al. (2010) Extensive enteric nervous system abnormalities in mice transgenic for artificial chromosomes containing Parkinson disease-associated alpha-synuclein gene mutations precede central nervous system changes. Hum Mol Genet. May 1;19(9):1633-50 Pelkonen A. et al. (2010) Stimulated dopamine overflow and alpha-synuclein expression in the nucleus accumbens core distinguish rats bred for differential ethanol preference. J Neurochem. 2010 Aug;114(4):1168-76. Alves et al. (2008) Striatal and nigral pathology in a lentiviral rat model of Machado-Joseph disease Hum Mol Genet. 2008 Jul 15;17(14):2071-83.
Specificity:
This antiserum specifically detects alpha synuclein. This antibody is known to react with alpha synuclein of human, mouse, rat and other rodents.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Purification:
Affinity purified and dialysed against phosphate buffered saline (PBS).
FUNCTION: Involved in redox regulation of the cell. Can reduce hydrogen peroxide and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. SUBUNIT: Homotetramer. May interact with HTR2A. SUBCELLULAR LOCATION: Cytoplasm. Lysosome. Also found in lung secretory organelles. MISCELLANEOUS: The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin . MISCELLANEOUS: Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress. SIMILARITY: Belongs to the ahpC/TSA family. Rehydrin subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Rat recombinant Peroxiredoxin-6
Applications:
ELISA,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB, ELISA. This antibody works superbly in Immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 1000 depending on tissue and detection method. For western blotting a dilution range of 1: 500 to 1: 2000 is recommended. A dilution of 1: 1000 to 1: 2000 is recommended for ELISA. This antiserum stains the cytoplasm of epithelial cells in the rat and mouse lung and rat and human brain astrocytes. It stains human brain astrocytes in Parkinson's and Alzheimer 's disease and the central core of some Lewy bodies in Parkinson's disease and dementia with Lewy bodies. Other tissues have not yet been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
This antibody has been shown to be specific for Peroxiredoxin-6 protein. Rat, human and mouse, other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Glutathione peroxidase 1 has a role in detoxification of hydrogen peroxide and is one of the most important antioxidant enzymes in humans. It exists as a homotetramer which localises to the cytoplasm. It belongs to the glutathione peroxidase family. Glutathione peroxidase 1 is one of few proteins in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. This protein has a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine repeats. The allele with five alanine repeats is significantly associated with breast cancer risk. Two alternatively spliced isoforms have been identified.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (RRYSRRFQTIDIEPDIEALL) corresponding to the amino acids 175-194 of human lutathione peroxidase 1 ( GPx-1) conjugated to diphtheria toxin has been used as the immunogen.
Applications:
ELISA,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. This antibody works superbly in Immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 2000 to 1: 20000 depending on tissue and detection method. For western blotting a dilution range of 1: 10000 to 1: 100000 is recommended. For ELISA, a dilution of 1: 2000 to 1: 20000 is recommended. This antiserum has extremely high titre, it stains human and rat brain microglia intensely and to some extent also stains neurons. Other tissues have not yet been tested. On Western blotting under reducing conditions recognises a 22kD protein in human brain tissue and Sigma glutathione peroxidase (G-4013) isolated from human erythrocytes. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
This antibody has been shown to be specific for glutathione peroxidase 1 (GPx-1) protein. Human and rat, other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Sheep anti-C-reactive protein Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
C-reactive protein has several roles associated with host defence such as; promoting agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. COFACTOR: Binds 2 calcium ions per subunit. C-reactive protein exists as a homopentamer. Pentaxin (or Pentraxin) have a discoid arrangement of 5 non-covalently bound subunits. There are 2 alternatively spliced isoforms. C-reactive protein is found in plasma and its concentration increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL-1 and IL-6.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with 0.01% Thimerosal
Host Animal:
Sheep
Species Reactivity:
Human
Immunogen:
Human recombinant C-Reactive protein
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. Extremely high titre antibody. For western blot a dilution of 1: 10,000 to 1: 100000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CRP; PTX1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
It has been shown to be specific for C-reactive protein by WB. Human, other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent.
LRRK2 is a member of the leucine-rich repeat kinase family. It role is yet unknown but it may play a role in the phoshorylation of proteins central to parkinson diseases. LRRK2 contains an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a mLK-like domain and a WD40 domain. LRRK2 is present in the cytoplasm but also associates with the mitochondrial outer membrane. Defects in LRRK2 are the cause of Parkinson disease 8 (PARK8). Parkinson disease is characterised by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. PARK8 is an autosomal-dominant late-onset parkinsonism, characterized by onset from 50 to 65 years, with slow progression and relatively benign course.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human
Immunogen:
A synthetic peptide (LKRKRKILSSDDSLRSS; aa 946-962) as part of human LRRK2 protein conjugated to the Blue Carrier Protein has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution range of 1:500-1:10000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Sheep anti-Gamma-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Gamma synuclein belongs to the synuclein family which are believed to be involve in the pathogenesis of neurodegenerative diseases. High levels of gamma synuclein have been identified in andvanced breast carcinomas suggesting a correlation between gamma synuclein overexpression and breast tumor development. Gama synuclein plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases. May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway. SUBUNIT: May be a centrosome-associated protein. SUBCELLULAR LOCATION: Cytoplasm; perinuclear region. Centrosome. Spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle. TISSUE SPECIFICITY: Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung. PTM: Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases. DISEASE: Brain iron accumulation type 1 (NBIA1, also called Hallervorden-Spatz syndrome), a rare neuroaxonal dystrophy, is histologically characterized by axonal spheroids, iron deposition, Lewy body (LB)-like intraneuronal inclusions, glial inclusions and neurofibrillary tangles. SNCG is found in spheroids but not in inclusions.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (EKEEVAEEAQSGGD) as part of human gamma synuclein protein (114-127) conjugated to diphteria toxid has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC, WB. A concentration of 2-5 µg/mL is recommended for both applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Persyn; Breast cancer-specific gene 1 protein; Synoretin; SR; SNCG
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Immunohistochemical/western blot anlysis indicate a high level of specificity for this antiserum for gamma synuclein. This antiserum is known to react with human and rat gamma synuclein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Purification:
Affinity purified and dialysed against PBS. Contains 0.02% sodium azide.
SUMO-1 binds to a wide range of target proteins as part of a post-translational modification system. Unlike ubiquitin, it does not seem to target protein for degradation, but is involved in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis, apoptosis, protein stability and signal transduction. SUBUNIT: Covalently attached to a number of proteins such as PmL, RANGAP1, HIPK2, SP100, p53, p73alpha, MDM2, JUN and DNMT3B. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, PIAS1, EXOSC9, TDG, RAD51 and RAD52. SUBCELLULAR LOCATION: Nucleus; nuclear membrane. Nucleus; nucleoplasm; nuclear speckle. Cytoplasm. SIMILARITY: Belongs to the ubiquitin family. SMT3 subfamily. SIMILARITY: Contains 1 ubiquitin-like domain. PTM: Cleavage of the last four amino acids of the carboxy-terminus of the precursor form by SENP1 or SENP2 is necessary for function. Several pseudogenes have been reported as well as a number of alternatively spliced isoforms.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (AKPSTEDLGDKKEGEY) as part of human SUMO-1 peptide (aa: 6-21) conjugated to diphtheria toxoid has been used as the immunogen. This antigen is homologous with SUMO-1 of rat.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:2000 to 1:4000 is recommended for immunohistochemistry and 1:4000 to 1:8000 for western blot. Cell lysate from Hela and NIH-3T3 cell lysates may be used as a positive control, and for IHC, lung carcinoma may be used. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Small ubiquitin-related modifier 1; Ubiquitin-like protein SMT3C; SMT3 homolog 3; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein UBL1; GAP-modifying protein 1; GMP1; Sentrin; SUMO1; SMT3C; SMT3H3; UBL1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum recognises human SUMO-1 and not ubiquitin. This antiserum is known to cross react with rat and human SUMO-1.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Sheep anti-Connexin-40 (Cx40) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
FUNCTION: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low molecular weight diffuse from one cell to a neighboring cell. SUBUNIT: A connexon is composed of a hexamer of connexins. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. TISSUE SPECIFICITY: Highly expressed in lung. SIMILARITY: Belongs to the connexin family. Alpha-type (group II) subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Rat
Immunogen:
A synthetic peptide consisting of amino acids 254 to 270 of the C-terminus of rat Cx40 (Cx40/254) conjugated to diphtheria toxoid has been used as the immunogen.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
<br><b>Immunohistochemistry</b>: Antibody detects Cxn 40 in rat tissues and arterial endothelial cells. The authors report that the density of Cx40 plaques was significantly greater in the caudal artery (CA) than in the thoracic aorta (ThA), whereas no such difference was seen for Cx37 and Cx43. Expression of Cx40 was absent from the media of both thoracic and caudal artery tissues (see Rummery, NM et al 2002 for more staining specifics). <br><b>Published Method</b>: Unfixed 10 µm thick sections cryosections or lightly fixed (2% paraformaldehyde in 0.1 mol/L sodium phosphate buffer) whole mount sections have been tested, see Rummery, NM et al 2002). Pretreatments include pre-incubation for 30 minutes in a blocking solution of 2% bovine serum albumin (BSA), 0.2% Triton-X in PBS, followed by primary antibody incubation. Antibody was used at 1:100 to 1:250 for 1 hour in the original work but Biosensis recommends optimizing the conditions for the best results. Original detection was via Cy3- conjugated anti-goat immunoglobulins (Jackson Immunoresearch Laboratories Inc, PA, 1:100) in 0.01% Triton-X in PBS, but other secondary conditions should work as well once optimized. In the original work the specificity of each antibody was tested by incubation either without primary antibody or with primary antibody that had previously been pre-incubated for 1 hour at room temperature with 10-fold excess by weight of the peptide against which the antibody was raised. (Adapted from Rummery, NM et al 2002).<br><b>Western Blot</b>: <U>Antibody is not recommended for western blots by Biosensis</u>, however, it does react in westerns with Cxn 40 specific material. The authors report that the antibody develops numerous bands in westerns blots, only some of which are removed upon peptide treatment (see Rummery, NM et al 2002). The Cx40/254 antibody specifically recognized a band of 40 kDa from lung, caudal artery (CA), and thoracic aorta (ThA) but not liver (online Figure VIIA, +/- peptide). In the lung, however, a band at 45 kDa also appeared to be reduced with Cxn 40 peptide addition.<br><b>Published Method</b>: Brain, heart, liver, lung, thoracic aorta and caudal arteries were removed from 5-6 week old Wistar rats and snap frozen in liquid nitrogen Tissues were ground under liquid nitrogen in a mortar and pestle and resuspended in 1mL of lysis buffer (1 mM NaHCO3 pH 7.05, 10 mM EDTA, 10 mM Iodoacetamide, 10 mM tetra-sodium pyrophosphate, 1 mM PMSF and 1 µg/mL each of antipain, aprotinin, pepstatin-A, chymostatin and leupeptin). Tissues were further disrupted by grinding in a polytron blender. Unbroken cells and large debris were removed by centrifugation at 1000 g for 5 minutes at 4oC, the supernatant was then removed and centrifuged at 3000 g for 5 minutes. The pellet was discarded, and the supernatant centrifuged at 20000 g for 15 minutes at 4°C. The supernatant was discarded, and the membrane-enriched pellet was resuspended in lysis buffer. Protein concentration was measured using the Bio-Rad protein assay kit. Membrane-bound connexins were subsequently solubilized by incubation in 2x SDS sample buffer (5% SDS, 125 mM Tris-Cl (pH 6.8), 20% glycerol, 2 mM _- mercaptoethanol, 0.1% (w/v) bromophenol blue) for 60 minutes at 37°C. Aliquots containing 5 µg of protein were separated by SDS-PAGE on 12% polyacrylamide gels and blotted onto PVDF membranes. Blots were probed with sheep antibodies against Cx40 (1:1000, Cx40/254) and detected via ECL using anti-Goat poly HRP secondary antibodies 1:4000, 1 hr. (<I>adapted from Rummery, NM et al 2002</I>).Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Gap junction alpha-5 protein; Cx40; Gja5; Cxn-40
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Original Reference Rummery NM, Hickey H, McGurk G, Hill CE. (2002) "Connexin37 is the major connexin expressed in the media of caudal artery. Arterioscler Thromb Vasc Biol. 22(9):1427-32. PMID: 12231561 This antibody is referred to as Cx40/254 in Rummery, NM et al 2002.
Specificity:
It is reported that the Cx40/254 antibody did not cross react with rat Cx43 but may with Cx45 in Western blots only (Rummery, NM et al 2002).
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Sheep anti-Connexin-45 (Cx45) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Connexin-45 is a component of gap junctions, which are composed of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low molecular weight diffuse from one cell to a neighboring cell. SUBUNIT: A connexon is composed of a hexamer of connexins. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. SIMILARITY: Belongs to the connexin family. Alpha-type (group II) subfamily. Alternatively spliced isoforms have been described.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human
Immunogen:
A synthetic peptide (QAYSHQN NPHGPRE) as part of human Connexin-45 protein (aa: 354-367) conjugated to diphtheria toxoid has been used as the immunogen.
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
<b>Immunohistochemistry:</b> Antibody detects sparse Cxn45-IR in caudal artery and heart tissue (see Rummery, NM et al 2002 for more staining specifics). Antibody was used at 1:100 to 1:250, but Biosensis recommends optimal dilutions/concentrations should be determined by the end user. In the original work the specificity of the antibody was shown by incubation either without primary antibody or with primary antibody that had previously been pre-incubated for 1 hour at room temperature with 10-fold excess by weight of the peptide against which the antibody was raised.<br><br><b>Western Blot:</b> Antibody is not recommended for western blotting by Biosensis, however, it does react in westerns with Cxn 45 specific material. The authors report that the antibody develops numerous bands in westerns blots, only some of which are removed upon peptide treatment (see Rummery, NM et al 2002). The Cx45/354 antibody revealed the presence of a specific 45-kDa band in all tissues tested, although it was very weak in the arteries. A higher molecular weight band, which was blocked by peptide, was also seen in the brain (see Rummery, NM et al 2002, online Figure VIIB, -/+ peptide).
Alternative Names:
Gap junction gamma-1 protein; Gap junction alpha-7 protein; Cx45; GJC1; GJA7
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Original Reference Rummery NM, Hickey H, McGurk G, Hill CE. (2002) "Connexin37 is the major connexin expressed in the media of caudal artery. Arterioscler Thromb Vasc Biol. 22(9):1427-32. PMID: 12231561 This antibody is referred to as Cx45/354 in Rummery, NM et al 2002.
Specificity:
Western blotting of cell membranes of COS cells transiently transfected with cDNA encoding Connexin-45 shows a singular band of molecular weight 47 kDa which is abolished by preincubation with the immunising peptide. No such band is detected in western blots of membranes from non-transfected cells or cells transfected with cDNA encoding Connexin-37 or Connexin-40. This antiserum reacts to human Connexin-45.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Seems to promotes the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Chicken,Human,Rat
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human, mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Primate,Rat
Immunogen:
Recombinant human NT4
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA (1 site), Western Blot, inhibition of biological activity in vitro/in vivo. Recommended to be used at a concentration of 2-10 µg/mL for immunohistochemistry, ELISA and Western blot and inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). Note that the concentration of NT4 is generally low in most tissues nevertheless, neonatal testes of rat can be used as a good positive control. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Beros J et al. (2021) Age Related Response of Neonatal Rat Retinal Ganglion Cells to Reduced TrkB Signaling in vitro and in vivo. Front Cell Dev Biol. 9:671087. Application: Rat, Block/Inhibit. Beros J (2020) Pretreatment of ovaries with collagenase before vitrification keeps the ovarian reserve by maintaining cell-cell adhesion integrity in ovarian follicles. PhD Thesis Application: Rat, Block/Inhibit. Feron F et al. (2008) Neurotrophin expression in the adult olfactory epithelium. Brain Res. 1196:13-21 Application: Rat, IHC.
Specificity:
Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by dot blot. Known to react with NT4 from rat and human, mouse and monkey.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Primate,Rat
Immunogen:
Recombinant human NT4
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA (1 site), Western Blot, dot blot, inhibition of biological activity in vitro/in vivo. Recommended to be used at a dilution of 1:500 to 1:2000 for immunohistochemistry, ELISA and Western blot. 1:10 to 1:50 for inhibition of biological activity in vitro. Use neat for in vivo studies at 5-10 µL/g body weight. Note that the concentration of NT4 is generally low in most tissues nevertheless, neonatal testes of rat can be used as a good positive control. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by 1-site ELISA. Known to react with NT4 from rat and human, mouse and monkey.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display sevvere movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Chicken,Human,Rat
Immunogen:
Recombinant human NT3
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. This antiserum will neutralise NT3 but not other neurotrophins. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A cross reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by 1-site ELISA. This antiserum is known to react with rat, chicken and human NT3.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display sevvere movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Chicken,Human,Rat
Immunogen:
Recombinant human NT3
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA, WB, dot blot, inhibition of biological activity. A dilution of 1:200 to 1:2000 is recommended for IHC, ELISA and western blot. For inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A cross reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by 1-site ELISA. This antiserum is known to react with rat, chicken and human NT3.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display sevvere movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Chicken,Human,Rat
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human, mouse and rat NT3 protein conjugated to BSA
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display sevvere movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Chicken,Human,Rat
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human, mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A dilution of 1:500 to 1:2000 is recommended for IHC, western blot. For inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Feron F et al (2008) Neurotrophin expression in the adult olfactory epithelium. Brain Res. 1196:13-21 Application: IHC ; Species: Rat
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A dilution of 1:1000-1:5000 is recommended for IHC, western blot and immunoblot; 1:15000 for ELISA; for inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily. SIMILARITY: Contains 1 bZIP domain
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Hamster,Human,Rabbit,Rat
Immunogen:
A synthetic peptide (MFSGFNADYEASSSRC; aa 2-17) conjugated to diphtheria toxoid has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from cFos protein human, rat, mouse, hamster and cat.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IH on PFA fixed, frozen tissues. Not yet tested on formalin fixed, paraffin embedded tissue but expected to react. Affinity purified antibody is extremely powerful. A concentration of 1 -5 µg/mL is recommended most uses with short (1-8 hour) incubations. If using enhanced brightfield or amplified detection methods dilutions and long primary antibody incubations dilutions will need to be increased substantially to inhibit background staining. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.<br><br>Not recommended for western blotting applications. Mouse monoclonal antibody M-1752-100 or rabbit polyclonal antibody R-1751-50 are excellent alternatives for western blotting.
Alternative Names:
Proto-oncogene protein cFOS; c-FOS
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum shows a high level of specificity for cFOS confirmed by immunohostochemistry. This antiserum is known to react with rat, rabbit and hamster cFOS.
Storage:
Store lyophilized product at 2-8°C. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Sheep anti-Pan-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Detects human alpha-, beta-, and gamma synuclein proteins. A family of homologous proteins known as alpha-, beta-, and gamma-synuclein are abundantly expressed in brain, especially in the presynaptic terminal of neurons. Although the precise function of these proteins remains unknown, alpha-synuclein has been implicated in synaptic plasticity associated with avian song learning as well as in the pathogenesis of Parkinson's disease (PD), dementia with LBs (DLB), some forms of Alzheimer's disease (AD), and multiple system atrophy (MSA).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (AKEGVVAAAEKTKQGV) as a consensus part of human alpha-, beta-, and gamma synuclein proteins conjugated to diphteria toxoid has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC, WB. A concentration of 3 µg/mL is recommended for immunohistochemistry (frozen & paraffin embedded sections) and 1 µg/mL for western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Overlap specific immunohistochemical staining of alpha-, beta- and gamma synucleins This antiserum recognises human and rat alpha-, beta- and gamma synucleins.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Purification:
Affinity purified and dialysed against PBS. Contains 0.02% sodium azide.
Sheep anti-Beta-synuclein Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Beta-synuclein is a non-amyloid component of senile plaques found in Alzheimer disease. It could act as a regulator of SNCA aggregation. It protects nerurons from staurosporine and 6 hydroxy dopamine -stimulated capspase activation in a p53-dependent manner. It localises to the cytoplasm and it is predominantly expressed in the brain where it is most concentrated in presynaptic nerve terminals. This protein is phosphorylated. This protein is also associated with the disease Brain iron accumulation type 1 (NBIA1).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (IEPLMEPEGSYEDPPQE) as part of human beta synuclein protein (aa: 108-125) conjugated to diphteria toxid has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:3000 to 1:8000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
SNCB
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Known to be specific for beta synuclein. This antiserum is know to react with human, rat and other rodent beta synuclein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Sheep anti-Ancient ubiquitous protein 1 (AUP1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
AUP1 contains a domain with homology to the ancient conserved region of the archain 1 gene and a domain thay may be involved in binding ubiquitin-conjugating enzymes. The unprocessed precusor is of 476 amino acids in length and has an estimated molecular weight of 53 kDa. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing.TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: Belongs to the AUP1 family. SIMILARITY: Contains 1 CUE domain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Bovine,Human,Rat,Sheep
Immunogen:
A synthetic peptide (HVFLVSCALPDSV) corresponding to the amino acids 48-60 of human ancient ubiquitous protein 1 conjugated to Blue carrier protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from bovine, mouse and rat.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. Use at a dilution of 1:500 to 1:6000. This antiserum works superbly in both paraffin embedded and frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
AUP1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum is specific for ancient ubiquitous protein 1. This antibody is known to react with bovine, sheep, human and rat ancient ubiquitous protein 1. Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily. SIMILARITY: Contains 1 bZIP domain
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Hamster,Human,Rabbit,Rat
Immunogen:
A synthetic peptide (MFSGFNADYEASSSRC; aa 2-17) conjugated to diphtheria toxoid has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from cFos protein human, rat, mouse, hamster and cat.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC: Use at an amount of 10 µg/mL with an incubation time of 1-3 days at 4ºC. This antiserum works in paraffin and 4% PFA fixed frozen sections. Penetration is the key to success. Over-fixed tissue is problematic. Not recommended for western blotting applications. Mouse monoclonal antibody M-1752-100 or rabbit polyclonal antibody R-1751-50 are excellent alternatives for western blotting. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
c-Fos; Proto-oncogene protein cFOS; cellular oncogene fos; G0/G1 switch regulatory protein 7; FOS; G0S7
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum shows a high level of specificity for c-FOS confirmed by immunohistochemistry. This antiserum is known to react with rat and rabbit and hamster cFOS.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
CNTF is a survival promoting factor for different types of neurons in vitro and in vivo. The essential structural features for the biological function of human CNTF were investigated by Thier, M. et al. They showed that deletion of 14 N-terminal and 18 C-terminal amino acids significantly increased bioactivity compared to wild-type CNTF. FUNCTION: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Nervous system. PHARMACEUTICAL: CNTF is being tested under the name Axokine by Regeneron Pharmaceuticals for treatment of human motor neuron diseases, such as amyotrophic lateral sclerosis (ALS). As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. SIMILARITY: Belongs to the CNTF family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human CNTF
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB, ELISA. A dilution of 1:500 to 4000 is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Ciliary neurotrophic factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody specifically detects CNTF shown by western blot. This antiserum to known to react with rat, mouse and human CNTF protein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Sheep anti-Alpha-synuclein Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. Alpha synuclein has been associated with several neurodegenerative diseases. A point mutation in the gene coding for the alpha-synuclein protein was the first discovery linking this protein to a rare familial form of Parkinson's disease (PD). Subsequently, other mutations in the alpha-synuclein gene have been identified in familial PD. The aggregated proteinaceous inclusions called Lewy bodies found in PD and cortical Lewy body dementia (LBD) were discovered to be predominantly alpha-synuclein. Aberrant aggregation of alpha-synuclein has been detected in an increasing number of neurodegenerative diseases, collectively known as synucleopathies. Alpha-synuclein exists physiologically in both soluble and membrane-bound states, in unstructured and alpha-helical conformations, respectively. The physiological function of alpha-synuclein appears to require its translocation between these subcellular compartments and interconversion between the 2 conformations. Abnormal processing of alpha-synuclein is predicted to lead to pathological changes in its binding properties and function.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide (CMPVDPDNEAYEMPSEE) as part of human alpha synuclein (aa: 116-131) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC with 1:1000 to 1:2000 dilution. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Wu W et al. (2022) "Intermediate-length CGG repeat expansion in NOTCH2NLC is associated with pathologically confirmed Alzheimers disease" Neurobiol. Aging 80(1):447-458; Application: IHC Species: Human Cong C et al. (2021) "Contribution of Alzheimer's Disease Neuropathologic Change to the Cognitive Dysfunction in Human Brains with Lewy Body-Related Pathology." J Alzheimers Dis. 80(1):447-458; Application: IHC Species: Human Zhang W et al. (2020) "Contribution of Alzheimer's Disease Neuropathologic Change to the Cognitive Dysfunction in Human Brains with Lewy Body-Related Pathology." Neurobiol. Aging [In press]; Application: IHC Species: Human
Specificity:
Immunohistochemistry shows a high specificity for alpha-synuclein. This antibody is known to react with human, rat and mouse alpha-synuclein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Sheep anti-Myelin basic protein (MBP) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of approximately 80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP). MBP is an 'intrinsically unstructured' protein with a high proportion (approximately 75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system. A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational. Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic. The message for myelin basic protein is selectively translocated to the ends of the cell processes. Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model. FUNCTION: Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity). SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Myelin membrane; peripheral membrane protein; cytoplasmic side. Cytoplasmic side of myelin. TISSUE SPECIFICITY: Found in both the central and the peripheral nervous system. PTM: At least 5 charge isomers; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4 and C5 (the least cationic form); are produced as a result of optional posttranslational modifications such as phosphorylation of serine or threonine residues, deamidation of glutamine or asparagine residues, citrullination and methylation of arginine residues. C1 and C2 are unphosphorylated, C3 and C4 are monophosphorylated and C5 is phosphorylated at two positions. SIMILARITY: Belongs to the myelin basic protein family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Guinea Pig,Human,Rat
Immunogen:
A synthetic peptide (YG SLPQKSQRSQ DENPVV, aa: 68-86) as part of guinea pig MBP protein conjugated to KLH
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1:4000 is recommended. Immunostaining for MBP of abnormal appearing oligodendrocytic process and cell bodies in demyelinating areas. This antibody recognises only areas of myelin degeneration when tested in injured spinal cord and lesioned sciatic nerves. It also stains discrete white matter in the brain of multiple system atrophy. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Myelin Basic Protein
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum recognizes MBP in demyelinated nerve tissues. Immunohistochemical analysis of lesioned rat spinal cord indictaes a high level of specificity for this antiserum. This antiserum reacts with human and rat MBP.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human GDNF
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. Recommended to be used at a dilution of 1:1000 to 1:3000 for Western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
No cross reactivity with NTN has been observed in western blot analysis. This antibody is known to react with human, mouse and rat GDNF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human GDNF
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC, WB. A dilution of 1 µg/mL is recommended for both applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
No cross reactivity with NTN has been observed in western blot analysis. This antibody is known to react with human, mouse and rat GDNF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human BDNF
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, Inhibition of biological activity in vitro/in vivo. Recommended to be used at a dilution of 1:200-2000 for immunohistochemistry on Zamboni's fixed frozen tissue; not recommended for formalin fixed paraffin embedded tissues. 1:10 to 1:50 for inhibition of biological activity in vitro. Use neat for in vivo studies at 5-10 µL/g body weight. Whole serum format will caused immune responses, purified format is preferred for most in vivo work. Not recommended for western blots. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A cross reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by one site ELISA. This antiserum recognises BDNF from rat, mouse and human.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
Recombinant human BDNF
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, Inhibition of biological activity in vitro/in vivo, ELISA. Recommended to be used at an amount of 1-10 µg/mL for immunohistochemistry on Zamboni's fixed, frozen tissue. Not recommended for paraffin embedded tissues. Primary use is for biological activity in vitro and in vivo. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antibody does not react to BDNF in western blot, thus western blot is not a recommended application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Beros J. et al. (2021) Age Related Response of Neonatal Rat Retinal Ganglion Cells to Reduced TrkB Signaling in vitro and in vivo. Front Cell Dev Biol. 9:671087. Beros J. (2020) Pretreatment of ovaries with collagenase before vitrification keeps the ovarian reserve by maintaining cell-cell adhesion integrity in ovarian follicles. PhD Thesis. Hayashida K., Eisenach JC. (2010) Spinal alpha2-adrenoceptor-mediated analgesia in neuropathic pain reflects brain-derived nerve growth factor and changes in spinal cholinergic neuronal function. Anesthesiology. 2010 Aug;113(2):406-12. Geremia NM. et al. (2010) Endogenous BDNF regulates induction of intrinsic neuronal growth programs in injured sensory neurons. Exp Neurol. 2010 May;223(1):128-42.
Specificity:
A cross reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by one site ELISA. Known to react with BDNF from rat and human.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Neurturin (NTN) is a member of the GDNF family of neurotrophic factors. This protein is a potent survival factor for several populations of central and peripheral neurons in mature and developing rodents. FUNCTION: Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. DISEASE: Defects in NRTN are a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, and possibly with other loci, defects in NRTN are involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human Neurturin (rh NTN)
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB, immunoblot, 1-site ELISA. Recommended to be used at a dilution of 1:2000-3000 for immunohistochemistry and Western blot, 1: 2000 to 1:4000 for ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neurturin; NRTN;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Dot blot shows no cross reactivity to GDNF. This antibody is known to react with human, mouse and rat. Not yet tested against other species.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Fibroblast growth factors (FGFs), a heparin binding growth factor, exhibit widespread mitogenic and neurotrophic activities in a variety of different cells including mesenchymal, neuroectodermal and endothelial cells. aFGF (FGF-1) and bFGF (FGF-2) are present in relatively high levels in CNS. aFGF is expressed by a subset of neuronal populations, while bFGF is expressed by astrocytes, both lack signal peptides. Human bFGF is a 17.2 kDa protein containing 155 amino acid residues. FUNCTION: The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. SUBUNIT: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2. MISCELLANEOUS: This protein binds heparin more strongly than does aFGF. SIMILARITY: Belongs to the heparin-binding growth factors family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human basic FGF
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC (frozen), WB. Recommended to be used at a dilution of 1: 1000 to 1:2000 for both applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Lee HJ et al (2010) Effects of sevoflurane on collagen production and growth factor expression in rats with an excision wound. Acta Anaesthesiol Scand. 2010 Aug;54(7):885-93.
Specificity:
A high level of specificity for bFGF was shown by immunohistochemistry for this antiserum. This antibody is known to react with human, mouse and rat basic FGF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Beta Amyloid Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
The beta Amyloid peptide is derived from the cleavage of the Amyloid precursor protein and varies in length from 39 to 43 amino acids. Beta amyloid peptides are the major constituents of the plaques and tangles that occur in Alzheimer's disease.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide (DAEFRHDSGYEVHH) conjugated to bovine serum albumin (BSA) corresponding to amino acid sequence 1-14 of mature human beta amyloid.
Applications:
ELISA
Antibody Isotype:
Mixed
Application Details:
ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human beta amyloid, cross reactivity to APP has not bee tested.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Fox3 is one of a family of mammalian homologues of Fox-1. The Fox proteins are about 46 kDa in size, and each includes a central highly conserved RRM type RNA recognition motif. Much interest has focused on Fox3 as a result of the recent finding that this protein corresponds to NeuN, a neuronal nuclear antigen. NeuN/Fox-3 has a function in RNA splicing and is expressed heavily and specifically in neuronal nuclei and cytoplasm. Our antibody was raised against the N-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli. We did not use full length Fox3 as immunogen since the three mammalian Fox homologues, namely Fox1, Fox2 and Fox3, include virtually identical RRM motifs. The N-terminal region of the three molecules are much more variable in the three molecules so antibodies specific for each of the three molecules can therefore be generated.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Peptide corresponding to amino acids 5-24 of human FOX3 coupled to KLH.
Applications:
ICC,IHC,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:500 - 1:1,000 is recommended for WB. A dilution of 1:5,000 - 1:10,000 is recommended for ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Feminizing locus on X; Fox-1; Fox3; NeuN; NeuN antigen; Neuronal nuclei antigen; Fox-1 homolog C; RNA binding protein fox-1 homolog 3
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Rabbit Polyclonal Antibody to Neurofilament NF-L C-terminus (Unconjugated), suitable for WB, IF, ICC.
Background Info:
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins may be included also. The major function of neurofilaments is likely to control the diameter of large axons. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70kDa with some variability across species. Antibodies to NF-L are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrigs disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and others.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Rabbit
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
C-terminal synthetic peptide of rat NF-L protein,(GEEEDTKESEEEEKKEESAGEEQAAKKKD) with an N-terminal Cys for coupling to KLH.
Applications:
FC,ICC,IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) and Flow Cytometry (2 ug per 10^6 cells). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:100 - 1:500 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neurofilament light polypeptide; 68 kDa neurofilament protein; Neurofilament triplet L protein
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Physical State:
Solid
Product references:
Bacioglu M et al. (2016) Neurofilament light chain in blood and CSF as a marker of disease progression in mouse models and in neurodegenerative diseases. Neuron. 27292537 Application: Mouse and Human.
Specificity:
No cross reactivity with other proteins.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Rabbit anti-Pan-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, FC.
Background Info:
A family of homologous proteins known as alpha-, beta-, and gamma-synuclein are abundantly expressed in brain, especially in the presynaptic terminal of neurons. Although the precise function of these proteins remains unknown, alpha-synuclein has been implicated in synaptic plasticity associated with avian song learning as well as in the pathogenesis of Parkinson's disease (PD), dementia with LBs (DLB), some forms of Alzheimer's disease (AD), and multiple system atrophy (MSA).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.2-7.6, containing 0.1% trehalose without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide (AKEGVVAAAEKTKQGV) as a consensus part of human alpha-, beta-, and gamma synuclein proteins has been used as the immunogen.
Applications:
FC,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (0.5-2 ?g/mL). Flow Cytometry (10-20 ?g/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Immunogen epitope location:
Amino acids 11-26 of human alpha-synuclein protein.
Immunogen length:
16 amino acids.
Physical State:
Solid.
Specificity:
This antibody recognises human, mouse, rat alpha-, beta- and gamma-synuclein.
Storage:
After reconstitution keep aliquots at -20°C for long-term stability. Working aliquots can be kept at 2-8°C for up to 4 weeks, or longer with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Product Validation Info:
Validated by western blotting and flow cytometry.
Purification:
Purified by affinity chromatography against the peptide immunogen.
Rabbit anti-Beta-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease (Ref: uniprot.org).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Full-length human recombinant ?-synuclein protein expressed in and purified from E. Coli.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
Western blotting (1:1,000-1:2,000) and Immunohistochemistry (frozen sections, 1:1,000-1:2,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-synuclein, ?-synuclein
Biosensis Brand:
Biosensis®
Cellular Localisation:
Intracellular, cytosolic.
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Immunogen length:
Full-length recombinant protein.
Physical State:
Solid.
Specificity:
Specific for ?-synuclein, does not cross-react with ?- or ?-synuclein.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Product Validation Info:
Validated by western blotting and immunohistochemical procedures.
Purification:
Affinity-purified from rabbit serum using the immunogen.
Rabbit anti-Microtubule-associated protein Tau (MAPT) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
FUNCTION: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. SUBCELLULAR LOCATION: Cytoplasm; cytosol. Cell membrane. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components. ALTERNATIVE PRODUCTS: 8 named isoforms produced by alternative splicing. Additional isoforms seem to exist. Isoforms differ from each other by the presence or absence of up to 5 of the 15 exons. One of these optional exons contains the additional tau/MAP repeat. TISSUE SPECIFICITY: Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system. DEVELOPMENTAL STAGE: Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain. DOMAIN: The tau/MAP repeat binds to tubulin. In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments and straight filaments, mainly composed of hyperphosphorylated forms of Microtubule-associated protein Tau. Defects in Microtubule-associated protein Tau are a cause of frontotemporal dementia and parkinsonism linked to chromosome 17, as well as a number of other neurodegenerative diseases.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Pig
Immunogen:
Recombinant human Microtubule-associated protein Tau, purified from E.coli.
Applications:
ELISA,WB
Antibody Isotype:
Mixed
Application Details:
WB and direct ELISA (Human). For WB a dilution of 1:500 is recommended. This antibody has been shown to detect the purified recombinant Tau expressed in E.Coli as well as a number of Tau isoforms in porcine cytosol. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; MAPT; MTBT1; TAU
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Ballaz S, Morales I, Rodriguez M, Obeso JA. (2013) Ascorbate prevents cell death from prolonged exposure to glutamate in an in vitro model of human dopaminergic neurons. J. Neurosci Res. Aug 30 2013 [E print]
Specificity:
Specificity was demonstrated by western blot. 20ng recombinant Tau is easily detected with smaller fragments likely representing degradation products from the purified protein, which has been expressed in E. Coli. This antiserum is known to react with human and pig Microtubule-associated Tau proteins. Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Green fluorescent protein (GFP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC.
Background Info:
The green fluorescent protein (GFP) is a 27 kDa protein isolated originally from the jellyfish Aequoria victoria. It has an endogenous fluorochrome activity with excitation maximum at 395nm and emission maximum at 509 nm, which is similar to that of fluorescein. GFP can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell. This GFP rabbit antibody was made against a recombinant GFP construct originating from an Aequoria species which was engineered to improve spectral properties and prevent oligomerization (1). This form of GFP, referred to as AcGFP, is 94% identical to the eGFP developed by Tsien and co-workers. The antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Rabbit
Species Reactivity:
Species Independent
Immunogen:
Recombinant AcGFP purified from E. coli
Applications:
IHC,WB
Antibody Isotype:
IgG
Application Details:
Immunocytochemistry (1:2,000 - 1:5,000) and Western Blot (1:1,000 - 1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Aequorea victoria green fluorescent protein
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
GFP, cross reactivity with other mutant forms is expected as immunogen was a whole molecule GFP
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
LRRK2 is a member of the leucine-rich repeat kinase family. Its role is yet unknown but it may play a role in the phoshorylation of proteins central to parkinson diseases. LRRK2 contains an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a mLK-like domain and a WD40 domain. LRRK2 is present in the cytoplasm but also associates with the mitochondrial outer membrane. Defects in LRRK2 are the cause of Parkinson disease 8 (PARK8). Parkinson disease is characterised by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. PARK8 is an autosomal-dominant late-onset parkinsonism, characterized by onset from 50 to 65 years, with slow progression and relatively benign course.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide (CLKRKRKILSSDDSLRSS) corresponding to amino acids 946 - 962 of the human LRRK2 protein conjugated to diptheria toxin was used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
IgG
Application Details:
IHC. A dilution range of 1:500 to 1:1000 is recommended. Other applications have not yet been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity was demonstrated by immunohistochemistry. The antibody stains positive tangles in inferior temporal cortex of human brain affected by Alzheimer's disease. This antiserum has been successfully tested in human. Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
C-terminal peptide of human IBA1 protein coupled to KLH.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:2,000-1:5,000); Immunocytochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Allograft inflammatory factor 1, AIF-1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human,reacts with Human, Rat, Mouse.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Ubiquitin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
Ubiquitin is a highly conserved 76 amino acid protein with an estimated molecular weight of 8.56 kDa which has a central role in regulated protein degradation. It is a protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Several types of polymeric chains can be formed depending on the lysine used for the assembly. Attachment to proteins as a polymer leads to their degradation by the 26S proteosome; a complex, multicatalytic cytosolic and nuclear protease. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatic structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats of which differ between species and strains. In some species there is a final amino-acid after the last repeat, here in bovine a Cys. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.2, 100mM NaCl, 15mM sodium azide.
Ubiquitin isolated from cow erythrocytes and conjugated to chicken gammaglobulins with glutaraldehyde.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for IHC, WB and immunoprecipitation. This antibody can be used for labelling formalin-fixed, paraffin-embedded tissue sections at a dilution range of 1:150 to 1:300. This antibody is also useful for investigating neurodegenerative diseases such as Alzheimer 's disease and Parkinson's disease. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
RPS27A; UBA52; UBB; UBC
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been determined by indirect ELISA against ubiquitin conjugated to keyhole limpet haemocyanin. The antibody does not react with kehole limpet haemocyanin alone. Specificity has also been demonstrated by WB against endometrial tissue homogenates. Cross reacts with human, baboon and rat ubiquitin. Other species have not been tested. Ubiquitin protein sequence is 100% conserved in all higher mammals and most eukarotes.
Storage:
After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Nestin Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity). Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Part of recombinant human protein (amino acids 315-630).
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human,reacts with Human. Other species not tested.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Methyl-CpG- binding protein 2 (MeCP2) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide (REEPVDSRTPVTERVS, aa: 471-486) of C-terminus of human protein.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000) Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CNPase
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human,reacts with Human, Mouse, Rat.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Laminin-111 Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organisation of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
Laminin-111 isolated from mouse EHS cells
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Laminin 1, Laminin _1_1_1.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Mouse,reacts with Mouse and Rat, other species not tested. This antibody recognizes laminin isotypes alpha-1 (440 kDa), beta-1 (220 kDa) and gamma-1 (220 kDa). It also binds laminin binding protein at 120 kDa which always co-expresses with laminin.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Ki-67 Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant human Ki-67 protein (amino acids 1,111-1,490) expressed in and purified from <i>E. coli.</i>
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
Western blotting (1:2,000-1:10,000) and Immunocytochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
KI-67
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human,reacts with Human only. Does not react with Mouse or Rat.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Full-length recombinant human protein
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:1,000-1:2,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human,reacts with Human, Rat, Mouse.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Calretinin is a calcium-binding protein which is abundant in auditory neurons. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Bovine,Human,Mouse,Rat
Immunogen:
Full-length recombinant human protein
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
Western blotting (1:5,000-1:10,000), Immunohistochemistry (1:5,000-1:10,000) and Immunocytochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CR, 29 kDa calbindin
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human,reacts with Human, Cow, Rat, Mouse. Antibody is specific for calretinin and does not recognize closely related proteins parvalbumin and calbindin as determined by Western Blotting.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Beta casein protein Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Beta-casein has an important role in determination of the surface properties of the casein micelles. it is cleaved into 3 chains (casoparan, casohypotensin and antioxidant peptide). Casoparan acts as a macrophage activator, increasing the phagocytic activity of macrophages and peroxide release from macrophages. It also acts as a bradykinin-potentiating peptide. Casohypotensin acts as a bradykinin-potentiating peptide and induces hypotension in rats. Acts as a strong competitive inhibitor of endo-oligopeptidase A. Antioxidant peptide has antioxidant activity (ref: uniprot.org.).<br /><br />Bovine milk contains two types of beta-casein protein, A2 or A1. Recent studies have shown that milk containing the A1 beta casein protein can contribute to issues including gastrointestinal discomfort after ingestion. There is some evidence of a link between ingestion of A1 beta casein protein and the development of Type 1 diabetes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS, pH 7.4, with 0.02% sodium azide as preservative. Refer to the product label for antibody concentration.
Host Animal:
Rabbit
Species Reactivity:
Bovine
Immunogen:
Bovine beta-casein peptides
Applications:
ELISA,WB
Antibody Isotype:
IgG
Application Details:
ELISA and western blotting (1:5,000 - 1: 20,000). Do not use skim milk for blocking and dilution of assay reagents, BSA is recommended. Biosensis recommends that optimal working dilutions should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Physical State:
Liquid.
Product references:
Indyk HE et al. (2021) The determination of intact ?-casein in milk products by biosensor immunoassay. J. Food Compos. Anal. 101:103946 Application: Immunoassay.
Specificity:
Bovine beta-casein. Detects both A1 and A2 beta-casein isoforms.
Storage:
Spin vial briefly before opening and centrifuge to remove any insoluble material. After opening maintain at -20°C in undiluted aliquots for up to 6 months. For short-term storage, keep aliquot at 2-8°C for up to one week. Avoid repeated freeze-thaw cycles.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide (C-ELLDEDQKVRPNEE) as a part of human BDNF precursor protein (aa: 69-82) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC, WB. 1-5 µg/mL is recommended for both applications, Flow Cytometry (2?g/10^6 cells). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Le Blanc J et al. (2020) Platelets Selectively Regulate the Release of BDNF, But Not That of Its Precursor Protein, proBDNF. Front Immunol. 11:575607 Application: Human, WB. Macias M et al. (2007) Locomotor exercise alters expression of pro-brain-derived neurotrophic factor, brain-derived neurotrophic factor and its receptor TrkB in the spinal cord of adult rats. Eur J Neurosci. 25(8):2425-44 Application: Rat
Specificity:
Used in western blot, this antiserum detects a 35 kDa band corresponding to the molecular weight of proBDNF. No cross reactivity with other proneurotrophins was detected. This antibody is known to react with human, mouse and rat proBDNF and also expected to recognise other mammalian proBDNF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily. SIMILARITY: Contains 1 bZIP domain (Ref: uniprot.org).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Full length, E.coli-derived recombinant human c-FOS protein.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
Immunocytochemistry (ICC): 1:2,000-1:10,000.<br><br>Immunohistochemistry (IHC): 1:20,000-1:50,000. This antibody has been shown to work on 4% PFA fixed mouse brain sections. Note that non-specific staining has been observed on tissue sections when using this antibody at dilutions of 1:5,000 or lower.<a class="newA" target="_blank" href="https://www.biosensis.com/documents/enhancedinfo/R-1751-50_IHC Method_as_at_March2018.pdf"> Click here </a> for instructions on use of this antibody in IHC on free-floating brain sections.<br><br>Western blotting (WB): 1:1,000-1:2,000. This antibody detects bands between 50-65 kDa, which only appear in stimulated cells. <br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Cellular oncogene fos; G0/G1 switch regulatory protein 7; cFOS
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Lin King JV (2020). Stinging Sensations: Activation Mechanisms of the Wasabi Receptor, TRPA1. PhD Thesis. Application: IHC (IF) . Species: Mouse. Lin King JV et al. (2019). A Cell-Penetrating Scorpion Toxin Enables Mode-Specific Modulation of TRPA1 and Pain. Cell. [Epub ahead of print]. Application: IHC . Species: Mouse.
Storage:
Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display sevvere movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Chicken,Human,Rat
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human (aa: 139-149), mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Activity-regulated gene 3.1 protein homolog (Arg3.1) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Arc (also termed activity-regulated cytoskeleton-associated protein or Arg3.1), is an effector immediate early gene whose upregulation has been demonstrated during events of synaptic plasticity. Arg3.1 expression is detectable in neuronal cell bodies and dendrites in the brain regions including striatum and cortex hippocampus, hypothalamus, amygdala.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide from the C terminal of human Arg3.1 protein (ARC protein) conjugated to Blue Carrier Protein. The sequence is homologous with mouse and rat form of Arg3.1.
Applications:
IHC-Frozen
Antibody Isotype:
IgG
Application Details:
IHC. A concentration of of 2-4 µg/mL is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specific for Arg3.1. Rat and Mouse. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Purification:
Protein G purified IgG
Target:
Activity-regulated gene 3.1 protein homolog (Arg3.1)
The protein named TrkB (also named Neurotrophic tyrosine kinase receptor type 2 (NTRK2), GP145-TrkB or Tropomyosin-related kinase B is a receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems and is important in the regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. TrkB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. TrkB is the primary receptor for BDNF (brain-derived neurotrophic factor. TrkB also binds NT4 and NT3 but less efficiently. Upon ligand-binding, the receptor undergoes homodimerization, autophosphorylation and activation. TrkB activation recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that each regulate distinct overlapping signaling cascades within cells. Through SHC1, FRS2, SH2B1, SH2B2, these activate the GRB2-Ras-MAPK cascade that regulates, for instance, neuronal differentiation including neurite outgrowth. These same effectors also control the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. TrkB, via activation of PLCG1 and the downstream protein kinase C-regulated pathways, also controls synaptic plasticity, and thus plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. One such consequence is that PLCG1 activation via TrkB is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. (Reference: www.uniprot.org)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized.
Host Animal:
Rabbit
Species Reactivity:
Chicken (Predicted),Human,Mouse,Rat
Immunogen:
Synthetic peptide immunogen, SNDDDSA[pS]PLHHIS
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
<b>Western Blotting (0.5 - 2 µg/mL).</b> Cell lysates or membrane preparations prepared from isolated brain or spinal cord tissues are recommended. This antibody works on skim milk blocked membranes, however, best results are obtained with a equal mixture of 2.5% skim milk / 2.5% highly purified BSA as blocking reagent and antibody diluent.<br><b>Immunohistochemistry (1 - 5 µg/mL).</b> Antibody has been shown to work on PFA fixed, frozen sections. TBS is preferred for buffer preparation.<br> Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Detects full-length TrkB phosphorylated at amino acid S479 in humans (S478 in mouse and rat).
Physical State:
Solid
Positive Control:
Retinoic acid- and BDNF-treated NSC34 cells
Product references:
Turnbull MT et al. (2018), Acute Down-regulation of BDNF Signaling Does Not Replicate Exacerbated Amyloid-? Levels and Cognitive Impairment Induced by Cholinergic Basal Forebrain Lesion. Front Mol Neurosci. 2018 Feb 22;11:51. Species: Mouse ; Application: WB, hippocampal lysates. Matusica D et al. (2016), Inhibition of motor neuron death in vitro and in vivo by a p75 neurotrophin receptor intracellular domain fragment. J Cell Sci. 2016 Feb 1;129(3):517-30. doi: 10.1242/jcs.173864. Epub 2015 Oct 26. Species: Mouse ; Application: WB, spinal cord lysates.
Specificity:
Human TrkB (pS478). Antibody has been shown to be specific for TrkB phosphorylated on serine 478 by phospho-peptide absorption dot blots, and on cell lysates from cell lines induced with retinoic acid and BDNF. Antibody detects a clear band in retinoic acid (RA) and BDNF-treated NSC34 cell lysates at ~120 kDa only, demonstrating that the phosphorylated TrkB receptor is being detected. Additional non-specific bands at lower molecular weight may be observed in lysates and homogenates with the antibody and these bands have not been characterized.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Product Validation Info:
Antibody is specific for TrkB serine phosphorylation at amino acid 478/479 (rodents/human). Lambda-phosphatase treatment obliterates positive staining.
The protein named TrkB (also named Neurotrophic tyrosine kinase receptor type 2 (NTRK2), GP145-TrkB or Tropomyosin-related kinase B is a receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems and is important in the regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. TrkB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. TrkB is the primary receptor for BDNF (brain-derived neurotrophic factor. TrkB also binds NT4 and NT3 but less efficiently. Upon ligand-binding, the receptor undergoes homodimerization, autophosphorylation and activation. TrkB activation recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that each regulate distinct overlapping signaling cascades within cells. Through SHC1, FRS2, SH2B1, SH2B2, these activate the GRB2-Ras-MAPK cascade that regulates, for instance, neuronal differentiation including neurite outgrowth. These same effectors also control the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. TrkB, via activation of PLCG1 and the downstream protein kinase C-regulated pathways, also controls synaptic plasticity, and thus plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. One such consequence is that PLCG1 activation via TrkB is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. (Reference: www.uniprot.org)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized.
Host Animal:
Rabbit
Species Reactivity:
Chicken (Predicted),Human,Mouse,Rat (Predicted)
Immunogen:
Synthetic peptide immunogen, AKASPV[pY]LDILG
Applications:
WB
Antibody Isotype:
IgG
Application Details:
<b>Western Blotting (0.5 2 µg/mL).</b> High skim milk concentration (5%) causes suppression of pY817 signal, suggesting that 5% skim milk should be avoided as blocking buffer and antibody diluent. Strongest signal is obtained in 5% BSA blocking buffer, however, many non-specific bands are present. An equal mixture of skim milk and BSA (2.5% each) appears to provide the best compromise between signal and noise. However, optimization of blocking condition is recommended for each particular sample, with excess of BSA over skim milk likely to be beneficial for best results.<br><br>Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Detects full-length TrkB phosphorylated at amino acid Y817 in humans (Y816 in mouse and rat).
Physical State:
Solid
Positive Control:
Retinoic acid- and BDNF-treated NSC34 cells
Specificity:
Human TrkB (pY817). Antibody has been shown to be specific for TrkB phosphorylated on tyrosine 817 by phospho-peptide absorption dot blots, and on cell lysates from cell lines induced with retinoic acid and BDNF. Antibody detects a clear band in retinoic acid (RA) and BDNF-treated NSC34 cell lysates at ~120 kDa only, demonstrating that the phosphorylated TrkB receptor is being detected. While not fully tested, this antibody may detect phosphorylated TrkA (pY791/794, human/rodent) and TrkC (pY834/820/859, human/mouse/rat) due to high degree of amino acid homology surrounding the phosphorylation site.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Product Validation Info:
Antibody detects a clear band in retinoic acid (RA) and BDNF-treated NSC34 cell lysates at ~120 kDa only
Rabbit anti-Neuropeptide Y (NPY) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Neuropeptide Y is a 36 amino acid neurotransmitter found in the brain and autonomic nervous system. It has been associated with numerous physiologic processes in the brain, including the regulation of energy balance, memory and learning, and epilepsy. The main effect is increased food intake and decreased physical activity. It is also implicated in the secretion of gonadotrophin-release hormone. It is secreted by the hypothalamus and it is one of the most abundant peptides in the nervous system. It is also found in some chromaffin cells of the adrenal medulla. The sheep form of NPY is 94% homologous with human NPY.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat,Sheep
Immunogen:
Sheep Neuropeptide Y (29-64: NB. includes only the NPY peptide and not either of the flanking peptides) conjugated to keyhole limpet hemocyanin.
Corder KM et al. (2018) Prefrontal cortex-dependent innate behaviors are altered by selective knockdown of Gad1 in neuropeptide Y interneurons. PLoS One. 2018 Jul 19;13(7):e0200809 Application: IHC/IF, frozen sections.
Specificity:
Specificity has been confirmed by direct ELISA against the antigen. Sheep, Human, Mouse & Rat.
Storage:
Maintain lyophilized material at 2-8°C for up to 12 months. After reconstitution keep aliquots at -20°C for up to six months or at 2-8°C with an appropriate antibacterial agent for up to 4 weeks. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Purification:
Affinity purified on antigen column. Refer to vial label for antibody concentration after reconstitution.
GPx-P belongs to the glutathione peroxidase family which are responsible for the detoxification of hydrogen peroxide. It protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. GPx-P is secreted in plasma and exists as a homotetramer.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (FEKGDVNGEKEQKFYTFLKN) corresponding to the amino acids 135-154 of human glutathione peroxidase 3 (GPx-P) conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from glutathione peroxidase 3 (GPx-P) protein in mouse, rat, orangutan and bovine.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC, only. A dilution o f 1: 1000 to 1: 5000 is recommended for immunohistochemistry depending on detection method but end user will need to determine dilution to suit application. Antigen retrieval is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
This antibody has been shown to be specific for glutathione peroxidase 3 protein (GPx-P) through staining and blocking studies. Human and rat, other species have not yet been ested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.05% sodium azide.
Host Animal:
Rabbit
Species Reactivity:
Human,Other Mammals (Predicted)
Immunogen:
Antibody was raised against a GST-tagged rhBDNF fusion protein and expressed in and purified from E. coli.
Applications:
ELISA
Antibody Isotype:
IgG
Application Details:
<b>Western Blotting (denaturing and reducing):</b> 0.2 to 1 µg/mL. Antibody detects 14 kDa mature BDNF monomer and 32 kDa proBDNF monomer in cell lysate and tissue homonenate. Antibody has only been tested on cell lysate and tissue homogenate of human origin. Acid-treated samples may give cleaner blots, and enhance signals for BDNF. R-1707-100 is not recommended for human serum samples. For human serum analysis, we recommend mouse monoclonal antibody to rhBDNF (M-1744-50/100), or rabbit polyclonal antibody to BDNF peptide 1-10 (R-083-100, whole serum; R-066-500, IgG).<br><br><b>Flow Cytometry:</b> ~2 µg per 10^6 cells, methanol fixation. Note: R-1707-100 cannot be used to distinguish the flow cytometry signal originating from mature BDNF versus proBDNF.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Brain-derived neurotrophic factor; Abrineurin
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human, rat and mouse BDNF. Expected to detect BDNF from other species due to sequence homology. No cross-reactivity with other neurotrophins.
Storage:
Store lyophilized antibody at 2-8°C protected from moisture. After reconstitution divide antibody into useful aliquots and keep aliquots at -20°C to -80°C for a higher stability. Working aliquots can be kept at 2-8°C for up to 1 month. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Phospho-calcium/calmodulin-dependent protein kinase type II subunit alpha, Thr253 (alpha-CaMKII, Thr253) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Calcium/calmodulin-stimulated protein kinase II (CaMKII) is composed of four different chains (alpha, beta, gamma, and delta) and is abundantly expressed in neurons. CaMKII is involved in regulating many aspects of neuronal function, including neurotransmitter synthesis and release, modulation of ion channel activity and cellular transport. The enzymatic function of CaMKII is regulated by its multiple phosphorylation sites and targeting to sub-cellular locations through interactions with protein binding partners. Phosphorylation of Thr253 has been identified in vivo and found to alter the interaction of CaMKII with binding partners, but not change its enzymatic activity. Thus, phosphorylation of Thr253 is suggested to modulate functional responses based on its binding partner and subsequently its sub-cellular localization.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Rat
Immunogen:
A synthetic peptide (NKmLpTINPSC) corresponding to the sequence around Thr253 (AA 249-257) in alpha-CaMKII was synthesized, purified to 95% purity by HPLC, analyzed by mass spectroscopy and coupled to diphtheria toxoid.
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (1:200 - 1:1000). Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Skelding KA et al (2012) J J Cereb Blood Flow Metab 32 (12), 2181-2192. Skelding KA et al (2010) Cell Signal 22 (5), 759-769. Gurd JW et al (2008) Brain Res 1218, 158-165. Migues PV et al (2006) J Neurochem 98 (1), 289-299.
Specificity:
Rat Predicted from gene analysis to react with human and mouse alpha-CaMKII.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Purification:
Whole serum
Target:
Phospho-calcium/calmodulin-dependent protein kinase type II subunit alpha, Thr253 (alpha-CaMKII, Thr253)
Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. GAPDH may have other roles in the activation of transcription and in the regulation of apoptosis as well as Alzheimer's disease and Huntington's disease. The immunogen used to raise this particular antibody was extensively purified pig GAPDH. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted)
Immunogen:
Full length recombinant human GAPDH expressed in and purified from E. coli
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:10,000-1:30,000 is recommended for WB. A dilution of 1:1,000-1:10,000 is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rabbit anti-Vimentin Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Vimentin is the major protein subunit of the 10nm or intermediate filaments protein found in many kinds of mesenchymal and epithelia cells. Vimentin is also found in many kinds of cells in tissue culture and in developing neuronal and astrocytic precursor cells in the central nervous system. Vimentin frequently forms copolymers with other intermediate filament proteins, such as GFAP (in many kinds of astrocytes), with desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted)
Immunogen:
Recombinant human Vimentin purified from E. coli.
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000-5,000 is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specific for the ~55 kDa Vimentin protein.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Lamin A/C Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
The Lamin proteins are members of the intermediate filament protein family but are located inside the nucleus rather than in the cytoplasm (1). The lamins function as skeletal components tightly associated with the inner nuclear membrane. Originally the proteins of the nuclear cytoskeleton were named Lamin A, B and C, from top to bottom as visualized on SDS-PAGE gels. Subsequently it was found that Lamins A and C were coded for by a single gene (2), while the Lamin B band may contain two proteins encoded by two genes now called Lamin B1 and Lamin B2. Lamin A has a mass of about 74 kDa while Lamin C is 65 kDa. The Lamin A protein includes 98 amino acids missing from Lamin C, while Lamin C has a C-terminal 6 amino acid peptide not present in Lamin A. Apart from these regions Lamin A and C are identical so that antibodies raised against either protein are likely to cross react with the other, as is the case with this monoclonal. Lamin polymerization and depolymerization is regulated by phosphorylation by cyclin dependent protein kinase 1 (CDK1), the key component of "maturation promoting factor", the central regulator of cell division. Activity of this kinase increases during cell division and is responsible for the breakdown of the nuclear lamina. Mutations in the LMNA gene are associated with several serious human diseases, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease type 2B1, and Hutchinson-Gilford progeria syndrome. This family of diseases belong to a larger group which are often referred to as Laminopathies, though some laminopathies are associated in defects in Lamin B1, B2 or one or other of the numerous nuclear lamina binding proteins. A truncated version of lamin A, commonly known as progerin, causes Hutchinson-Gilford progeria syndrome, a form of premature aging (3).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Rabbit
Species Reactivity:
Human,Other Mammals (Predicted)
Immunogen:
Full length recombinant human Lamin C
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
Immunocytochemistry (ICC) and Western Blotting (WB). A dilution of 1:1,000-1:5,000 is recommended for WB. A dilution of 1:1,000-1:5,000 is recommended for ICC. The optimal dilution should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Lamin A and Lamin C. The antibody reacts with a 74 kDa and 65 kDa band by Western blot on HeLa cell extract. It has also been used successfully for immunocytochemistry on HeLa cell cultures.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Spectrin, alpha II Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
The spectrin family of proteins were originally discovered as major components of the submembraneous cytoskeleton of osmotically lysed red blood cells (1). The lysed blood cells could be seen as clear red blood cell shaped objects in the light microscope and were referred to as red cell "ghosts". The major proteins of these ghosts proved to be actin, ankyrin, band 4.1 and several other proteins, including two major bands running at about 240 kDa and 260 kDa on SDS-PAGE gels. This pair of bands was named "spectrin" since they were discovered in these red blood cell ghosts (1). Later work showed that similar high molecular bands were seen in membrane preparations from other eukaryotic cell types. Work by Levine and Willard described a pair of about ~240-260 kDa molecular weight bands which were transported at the slowest rate along mammalian axons (2). They named these proteins "fodrin" as antibody studies showed that they were localized in the sheath under the axonal membrane, but not in the core of the axon (2; fodros is Greek for sheath). Subsequently fodrin was found to be a member of the spectrin family of proteins, and the spectrin nomenclature is now normally used (3). Spectrins form tetramers of two alpha and two beta subunits, with the alpha corresponding to the lower molecular weight ~240 kDa band and the beta corresponding to the ~260 kDa or in some case much larger band. Most spectrin tetramers are about 0.2microns or 200nm long, and each alpha and beta subunit has a cell type specific expression pattern. The basic structure of each spectrin subunit is the spectrin repeat, which is a sequence of about 110 amino acids which defines a compact domain contain three closely packed alpha-helices. Each spectrin subunit contains multiple copies of this repeat, with 20 in each of the alpha subunits. The beta I-IV subunits each contain 17 spectrin repeats, while the beta V subunit, also known as beta-heavy spectrin, contains 30 of these repeats. The various subunits also contain several other kinds of functional domain, allowing the spectrin tetramer to interact with a variety of protein, ionic and lipid targets. The alpha-subunits each contain one calmodulin like calcium binding region and one Src-homology 3 (SH3) domain, an abundant domain involved in specific protein-protein interactions. The beta subunits all have a N-terminal actin binding domain and may also have one SH3 domain and one pleckstrin homology domain, a multifunctional type of binding domain which in beta I spectrin at least binds the membrane lipid PIP2 (5). Spectrins are believed to have a function in giving mechanical strength to the plasma membrane since the tetramers associate with each other to form a dense submembraneous geodesic meshwork (3). They also bind a variety of other membrane proteins and membrane lipids, and the proteins they bind to are therefore themselves localized in the membrane. Diseases may be associated with defects in one or other of the spectrin subunits (6). For example, some forms of hereditary spherocytosis, the presence of spherical red blood cells which are prone to lysis, can be traced to mutations in some of the spectrin subunits (7). The alpha-II subunit is widely expressed in tissues but, in the nervous system, is found predominantly in neurons. The antibody can therefore be used to identify neurons and fragments derived from neuronal membranes in cells in tissue culture and in sectioned material.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
The antibody was raised against a mix of five recombinant constructs containing the entire C-terminal region of human alpha-II spectrin (amino acids 676-2,400).
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB) and Immunocytochemistry (IC). Suggested dilution for WB is 1:5,000-10,000 and 1:500-1,000 for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a 240 kDa band by Western blot on mouse sciatic nerve extract. Minor bands below may be seen and this likely represents in vivo proteolytic fragments of alpha-II spectrin. It has also been used successfully for immunocytochemistry.
Storage:
Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.
FUNCTION: Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. Lysosome. Also found in lung secretory organelles. MISCELLANEOUS: The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin . MISCELLANEOUS: Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress. SIMILARITY: Belongs to the ahpC/TSA family. Rehydrin subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Rat recombinant Peroxiredoxin-6. The sequence is homologous in human and mouse peroxiredoxin-6.
Applications:
ELISA,ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB, ELISA. This antibody works superbly in Immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 1000 depending on tissue and detection method. For western blotting a dilution range of 1: 1000 to 1: 4000 is recommended. A dilution of 1: 1000 to 1: 4000 is recommended for ELISA. This antiserum stains the cytoplasm of epithelial cells in the rat and mouse lung and rat and human brain astrocytes. It stains human brain astrocytes in Parkinson's and Alzheimer 's disease and the central core of some Lewy bodies in Parkinson's disease and dementia with Lewy bodies. Other tissues have not yet been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Walsh B. et al. (2010) Overexpression of Prdx6 and resistance to peroxide-induced death in Hepa1-6 cells: Prdx suppression increases apoptosis. Redox Rep. 2009;14(6):275-84. Gardiner F. et al. (2010) Induction of Prdx1 and Prdx6 in Liver Cellsby Serum and TPA. Intl J. Biol. Vol. 2, No. 1
Specificity:
This antibody has been shown to be specific for Peroxiredoxin-6 protein. Rat, human and mouse, other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Rabbit anti-Adenylate cyclase type III (ACIII) Polyclonal Antibody (Unconjugated), suitable for WB, IHC, ICC.
Background Info:
Adenylate cyclases are enzymes which interact with and are activated by the GTP bound alpha subunits of trimeric G-proteins. Activated adenylate cyclases are responsible for the production of the important "second messenger" signalling molecule cyclic-AMP, which is generated from ATP. The type III adenylate cyclase enzyme is localized in the membranes surrounding the cilia in neurons, and our antibody is an excellent marker of neuronal cilia in the brain and in cells in tissue culture. Adenylate cyclase type III is a large complex molecule of, in the human, 1145 amino acids with a deduced molecular weight of 129 kDa. The protein may be variably glycosylated, so that on SDS-PAGE and western blots it runs as a diffuse band of about 160 kDa in cortex and about 200 kDa in olfactory epithelium. The molecule has a complex structure, with 12 transmembrane domains and two cyclase domains. Each cyclase domain is immediately C-terminal to 6 transmembrane segments, but only the second, C-terminal cyclase is believed to be catalytically active.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Rabbit
Species Reactivity:
Human (Predicted),Mouse,Other Mammals (Predicted),Rat
Immunogen:
20 amino acid peptide identical to the C-terminus of rat ACIII (amino acids PAAFPNGSSVTLPHQVVDNP)
Applications:
ICC,IHC,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:1,000-1:2,000 is recommended for WB. A dilution of 1:5,000-1:10,000 is recommended for ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody stains a band at about 200 kDa in olfactory epithelium which is rich in cilia. Fewer cilia are found in frontal cortex, and the protein is less heavily glycosylated, and a less prominent band is seen at about 160 kDa. It has also been used successfully for immunocytochemistry on mixed neuron/glia cultures. The antibody has been directly tested for reactivity in rat. It is expected that it will work on human due to homology with the immunogen and possibly other mammal tissues (Human, horse, cow, pig, chicken, mouse).
Storage:
Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Nicastrin, N-terminal Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Nicastrin, a type 1 membrane glycoprotein, is an essential component of the gamma secretase complex which is critical for the cleavage of the amyloid precursor protein and other membrane proteins. Nicastrin is widely expressed in different tissue types. This antibody detects all processed forms of Nicastrin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4. Contains no preservative.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide (RGNSVERKIYIPL-C) corresponding to human Nicastrin [32-44] in the N-terminal region conjugated via additional C-terminal Cys to Diphtheria toxoid.
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
WB and IP. Suggested concentration of 3-10 µg/mL is recommended for WB. Human or mouse brain samples commonly prepared with reducing (50mM DTT), urea (2.3 M), SDS (1.5%) in 62.5 mM Tris-HCL pH 6.8 sample buffer (without boiling) heating to 50 C for 15 min. Unprocessed full length human Nicastrin is 709 amino acids, however this protein contains an N-terminal signal peptide which is considered to undergo cleavage during processing and transit to the cell plasma membrane, in addition the protein undergoes glycosylation to produce a glycoprotein of about 145 kDa apparent MW by SDS PAGE. Biosensis recommends that the optimal working dilution should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed by WB using peptide absorption.
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Rabbit anti-Nicastrin, Central region Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Nicastrin, a type 1 membrane glycoprotein, is an essential component of the gamma secretase complex which is critical for the cleavage of the amyloid precursor protein and other membrane proteins. Nicastrin is widely expressed in different tissue types. This antibody reacts with immature forms of Nicastrin. Detection of higher mol. wt. mature forms is likely to be blocked by glycosylation in this region of the protein.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4. Contains no preservative.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide (C-QGETFDYIGSSRMVYD) corresponding to human Nicastrin [331-346] in the central region conjugated via additional N-terminal Cys to Diphtheria toxoid.
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
WB and IP. Suggested concentration of 3-10 µg/mL is recommended for WB. Human or mouse brain samples commonly prepared with reducing (50 mM DTT), urea (2.3 M), SDS (1.5%) in 62.5 mM Tris-HCL pH 6.8 sample buffer (without boiling) heating to 50 C for 15 min. Unprocessed full length human Nicastrin is 709 amino acids, however this protein contains an N-terminal signal peptide which is considered to undergo removal/cleavage during processing and transit to the cell plasma membrane, in addition the protein undergoes glycosylation to produce a glycoprotein of about 145 kDa apparent MW by SDS PAGE. Biosensis recommends that the optimal working dilution should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed by WB using peptide absorption.
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Rabbit anti-Nicastrin, C-terminal Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Nicastrin, a type 1 membrane glycoprotein, is an essential component of the gamma secretase complex which is critical for the cleavage of the amyloid precursor protein and other membrane proteins. Nicastrin is widely expressed in different tissue types. This antibody detects all processed forms of Nicastrin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4. Contains no preservative.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide (C-NAKADVLFIAPREPGAVSY) corresponding to human Nicastrin [691-709] in the C-terminal region conjugated via additional N-terminal Cys to Diphtheria toxoid.
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
WB and IP. Suggested concentration of 3-10 µg/mL is recommended for WB. Human or mouse brain samples commonly prepared with reducing (50mM DTT), urea (2.3 M), SDS (1.5%) in 62.5 mM Tris-HCL pH 6.8 sample buffer (without boiling) heating to 50 C for 15 min. Unprocessed full length human Nicastrin is 709 amino acids, however this protein contains an N-terminal signal peptide which is considered to undergo cleavage during processing and transit to the cell plasma membrane, in addition the protein undergoes glycosylation to produce a glycoprotein of about 145 kDa apparent MW by SDS PAGE. Biosensis recommends that the optimal working dilution should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed by WB using peptide absorption.
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Rabbit anti-Beta-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Beta-synuclein is a soluble cytoplasmic protein associated with synaptic vesicles and a member of the synuclein family. Mutations in alpha-synuclein cause early onset Parkinson's disease. Expression of beta synuclein may modulate alpha-synuclein aggregation found in Parkinson's disease.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4. Contains no preservative.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide (AQEAAEEPLIEPLME-C) corresponding to human _-synuclein [99-113] in the C-terminal domain conjugated via additional C-terminal Cys to Diphtheria toxoid.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
WB and IHC. A dilution of 1:500 to 1:1,000 is recommended for Western blot. _-synuclein is a soluble protein of 134 amino acids and detected with 17 kDa mobility by western blotting. By IHC the antibody detects synaptic sites in human brain formaldehyde-treated frozen tissue. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed by WB using soluble mouse and human brain extracts, reactivity for major product diminished by peptide absorption. Does not detect alpha-synuclein as tested with recombinant protein and does not react with Lewy bodies in human Dementia with Lewy Bodies or Parkinson's disease brain tissue sections.
Storage:
Lyophilized at 2-8°C. After reconstitution, store at -20°C in undiluted aliquots for up to 6 months. The antibody may be stored short term at 2-2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Rabbit anti-DJ-1/PARK7 Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Autosomal recessive mutations in DJ-1 cause early-onset familial Parkinson's disease. DJ-1 is considered a redox-sensitive cytoplasmic protein found in brain as well as other cell types.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4. Contains no preservative.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide (ASKRALVILAKGAEE-C) corresponding to human DJ-1 [2-16] in the N-terminal domain conjugated via additional C-terminal Cys to Diphtheria toxoid.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
WB and IHC. Suggested dilution of 1:5,000 is recommended for WB. DJ-1 is a soluble protein of 189 amino acids and detected with 23 kDa mobility by western blotting. The suggested dilution for IHC is 1:100. Detected astrocyte cytoplasmic labelling in human brain formaldehyde-treated tissue. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
PARK7
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed by WB using soluble mouse and human brain extracts, reactivity for major product diminished by peptide absorption.
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Rabbit anti-Presenilin 2 loop region Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Autosomal dominant mutations in presenilin 2 are the second major cause of early-onset familial Alzheimer's disease. Presenilin 2 is a multi-transmembrane protein which undergoes endoprotelysis to form an N-terminal fragment of about 29 kDa and C-terminal fragment of about 22 kDa. Presenilin 2 forms the catalytic core of the gamma-secretase complex which cleaves type 1 transmembrane proteins including the amyloid precursor protein to generate the C-terminus of the amyloid beta peptide.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4. Contains no preservative.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide (KLDPSSQGALQLPYDPEMEEDSYDSFGEP-C) corresponding to human PS1 [306-334] in the loop region conjugated via additional C-terminal Cys to Diphtheria toxoid.
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
WB and IP. Suggested dilution of 1:1,000 is recommended for WB. Full length presenilin 2 (448 aa) has relative MW of about 45 kDa, with this antibody most commonly detected as cleaved CTF of 22 kDa with this antibody. Human or mouse brain samples commonly prepared with reducing agent (50mM DTT), urea (2.3 M), SDS (1.5%) in 62.5 mM Tris-HCL pH 6.8 sample buffer (without boiling) heating to 50 C for 15 min. The suggested dilution for IP is 1:100 . Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
AD3LP, AD5, E5-1, STM-2
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed by Western blot using mouse and human brain and knock down of presenilin 2 in vitro using siRNA see ref 6 below. Not reactive with presenilin 1.
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Rabbit anti-Presenilin 1 loop region Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Presenilin-1 (PSEN1) is a multi-pass membrane protein and component of the gamma-secretase complex. PSEN1 is thought to play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. It may also play a role in hematopoiesis. Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3), a familial early-onset form of Alzheimer disease (Ref:SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4. Contains no preservative.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide (GDPEAQRRVSKNSKYNA-C) corresponding to human PS1 [301-317] in the loop region conjugated via additional C-terminal Cys to Diphtheria toxoid.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
Western Blot. Suggested dilution of 1:1,000 is recommended for WB. Full length presenilin 1 (467 aa) has relative MW of about 45 kDa, with this antibody most commonly detected as cleaved CTF of 19 kDa. Human or mouse brain samples commonly prepared with reducing agent (50mM DTT), urea (2.3 M), SDS (1.5%) in 62.5 mM Tris-HCL pH 6.8 sample buffer (without boiling) heating to 50 C for 15 min. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Presenilin 1; PS-1; Protein S182; PS1-CTF12; PSEN1; AD3; PS1; PSNL1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed by Western blotting using transfected cells, presenilin 1 knock-out mouse cells and mouse and human brain.
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
SOD1 binds copper and zinc ions ans is one of two isozymes responsible for destroying free superoxide radicals which are normally produced within the cells and which are toxic to biological systems. SOD1 is a soluble cytoplasmic protein, acting as a homodimer to convert superoxide radicals to molecular oxygen and hydrogen peroxide. Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) which is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A peptide (HEKADDLGKGGNEESTKTG) corresponding to the amino acids 121-139 of human superoxide dismutase (SOD1) conjugated to diphtheria toxin has been used as the immunogen.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. This antibody works superbly in immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 1000 depending on tissue and detection method. For western blotting a dilution range of 1: 1000 to 1: 4000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Superoxide dismutase; SOD1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody has been shown to be specific for superoxide dismutase (SOD1) protein. Human. Does not detect mouse and rat SOD1.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson's disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Concentrated ammonium sulfate in PBS pH 7.4 containing no preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant human MANF protein produced using CHO-based cell line. Immunogen is purified from cell culture supernatant.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
Western blot (WB) at a suggested dilution of 1:2,000 - 1:6,000. Immunohistochemistry (IHC) and Immunofluorescence (IF). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
ARMET, ARP
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
MANF (Mesencephalic astrocyte-derived neurotrophic factor). No cross-reactivity in MANF-knockout mouse. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB.
Storage:
Store antibody stock solution at 2-8°C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. See reconstitution instructions for more information.
CDNF (conserved dopamine neurotrophic factor) is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007; Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson's disease. When administered after 6-OHDA or MPTP -lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Concentrated ammonium sulphate in PBS pH 7.4 containing no preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant human CDNF protein produced using CHO-based cell line. Purified from cell culture supernatant.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
Western blot (WB) at a suggested dilution of 1:6,000 - 1:10,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
ARMETL1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
CDNF (conserved dopamine neurotrophic factor)
Storage:
Store at 2-8°C upon receipt; Do not freeze. See reconstitution instructions for handling information.
FUNCTION: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. SUBUNIT: Adaptor protein complex 1 (AP-1) is an heterotetramer composed of two large adaptins (gamma1/AP1G1 or gamma2/AP1G2 and beta1A/AP1B1 or beta1B/AP1B1), a medium adaptin (mu1A/AP1M1 or mu1B/AP1M2) and a small adaptin (sigma1A/AP1S1 or sigma1B/AP1S2 or sigma1C/AP1S3). SUBCELLULAR LOCATION: Golgi apparatus. Cytoplasmic vesicle; cytoplasmic vesicle membrane; peripheral membrane protein; cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Widely expressed. DISEASE: Deletion of the AP1B1 gene may play a role in the tumorigenesis of meningiomas. SIMILARITY: Belongs to the adaptor complexes large subunit family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (C-KKGEIFELKAELNSDKKEK) corresponding to the amino acids 11-29 of human AP-1 complex subunit beta-1 conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from AP-1 complex subunit beta-1 protein in mouse, rat and bovine.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. This antibody works superbly in immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 1000 to 1: 5000 depending on tissue and detection method. For western blotting a dilution range of 1: 100 to 1: 10000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Adapter-related protein complex 1 beta-1 subunit; Beta-adaptin 1; Adaptor protein complex AP-1 beta-1 subunit; Golgi adaptor HA1/AP1 adaptin beta subunit; Clathrin assembly protein complex 1 beta large chain; AP1B1; ADTB1; BAM22
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody has been shown to be specific for AP-1 complex subunit beta-1. Rat and human, other species have not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Rabbit anti-Neurturin (NTN) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
<span itemprop="description">Neurturin (NTN) is a member of the GDNF family of neurotrophic factors. This protein is a potent survival factor for several populations of central and peripheral neurons in mature and developing rodents. FUNCTION: Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. DISEASE: Defects in NRTN are a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, and possibly with other loci, defects in NRTN are involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.</span>
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Concentrated ammonium sulphate in PBS pH 7.4.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant human NRTN protein produced using CHO-based suspension cell line. Protein was purified from the cell culture supernatant.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
Western blot (WB) at a suggested dilution of 1:500-1:1,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Antibody does not show cross-reactivity to other GDNF-family proteins.
Storage:
Store at 2-8°C upon receipt. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use.
Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans Golgi network to lysosomes. Together with medium and small subunits, adaptins form a heterotetrameric complex called an adaptor whose role is to promote the formation of clathrin-coated pits and vesicles and to recognise sorting signals within the cytosolic tails of transmembrane cargo molecules. Gamma-adaptin protein belongs to the adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network and/or endosomes. SUBCELLULAR LOCATION: Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane.
A synthetic peptide (IRLRELIRTIRTARTQAEER) corresponding to the amino acids 5-24 of human AP-1 complex subunit gamma-1 conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from AP-1 complex subunit gamma-1 protein in mouse, rat, dog and zebra fish.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. This antibody works in immunohistochemistry on frozen or wax embedded tissue. Antigen retrieval has been used in testing but may not be necessary. Typical working dilution: Light microscopy 1/500 to 1/2000 depending on tissue and detection method; Immunofluorescence 1/50 to 1/200; Western blotting 1/1000 to 1/4000 is recommended depending on detection method. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
AP-1 complex subunit gamma-1; Adapter-related protein complex 1 subunit gamma-1; Gamma1-adaptin; Adaptor protein complex AP-1 subunit gamma-1; Golgi adaptor HA1/AP1 adaptin subunit gamma-1; Clathrin assembly protein complex 1 gamma-1 large chain; AP1G1; ADTG; CLAPG1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody has been shown to be specific for AP-1 Rat and human
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent.
Rabbit anti-mCherry Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
mCherry is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals). The mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28 kDa protein similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome. The original DsRed was engineered extensively in the Tsien lab to prevent it from forming tetramers and dimers and to modify and improve the spectral properties (1-3). The resulting monomeric protein is useful for applications such as Foerster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). Several further cycles of mutation, directed modification and evolutionary selection produced mCherry, which is monomeric and has an excitation maximum at 587 nm and and emission maximum at 610 nm (4).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Rabbit
Species Reactivity:
Species Independent
Immunogen:
Recombinant full length mCherry.
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:500 to 1:1,000 is recommended for WB. A dilution of 1:250 to 1:500 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a band at ~28-30 kDa corresponding to intact full-length mCherry by Western blot on HEK293 cells transfected with mCherry vector. It has also been used successfully for immunocytochemistry.
Storage:
Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Growth Associated Protein 43 (GAP-43) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
GAP43 is very abundant protein which is found concentrated in neurons. One group discovered it as one of three proteins which becomes unregulated during the regeneration of the toad optic nerve (1). Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 were independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14 kDa. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Rabbit
Species Reactivity:
Bovine,Human,Mouse,Rat
Immunogen:
C-terminal peptide 217-227 of rat and mouse GAP43, which is KEDPEADQEHA, to which an N terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein.
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB). A dilution of 1:5,000 - 1:20,000 is recommended. A dilution of 1:500-2,000 is recommended for Immunocytochemistry (ICC). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a 43 kDa band by Western blot on bovine cerebellum homogenate. The molecular weight of the protein recognized can vary (~43-57 kDa) depending on the species and the percentage acrylamide used in the SDS-PAGE gel. It has also been used successfully for immunocytochemistry.
Storage:
Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Clathrin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded.
Background Info:
Clathrin is a major protein component of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice to either the plasma membrane or the trans-Golgi network. These specialised organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium. Clathrin localises to the cytoplasmic face of coated pit and vesicle membranes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Rat
Immunogen:
A synthetic peptide (KAGLLQRALEHFTDLYDIKR) corresponding to the amino acids 619-638 of rat clathrin conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from clathrin protein in mouse, human and bovine.
Applications:
IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. This antibody works in immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 4000 depending on tissue and detection method. It is usual to use approximately a 10-fold increase for confocal microscopy i.e. a dilution range of 1: 100 to 1: 400.For western blotting a dilution range of 1: 1000 to 1: 8000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Clathrin heavy chain; Clathrin heavy chain 1; CLH-17; CLTC
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody has been shown to be specific for clathrin. Rat
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent.
Rabbit anti-Tyrosine Hydroxylase (TH) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. FUNCTION: Plays an important role in the physiology of adrenergic neurons. CATALYTIC ACTIVITY: L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin. COFACTOR: Fe(2+) ion. ENZYME REGULATION: Phosphorylation leads to an increase in the catalytic activity. PATHWAY: Catecholamine biosynthesis; first step. SUBUNIT: Homotetramer. PTM: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation. SIMILARITY: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Guinea Pig,Human,Mouse,Rat
Immunogen:
A synthetic peptide (PRFIGRRQSLIEDARK) as part of human Tyrosine Hydroxylase (63-78) conjugated to KLH has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from TH protein in rat (31-47).
Applications:
IHC-Frozen
Antibody Isotype:
IgG
Application Details:
IHC. A concentration of 4-10 µg/mL is recommended for this application. This is a superb antibody for detection of tyrosine hydroxylase containing neurons exhibiting an intense labelling with a negligible background. This antiserum has proven extremely useful for staining of catecholaminergic neurons. It stains nicely and intensely dendritic processes and fine nerve terminals. We recommend mouse or rat brain containing catecholaminergic neurons as a positive control for this antibody, for example brain stem or striatum. Western blotting: A concentration of 5-15 µg/mL is recommended.Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Pierre S.R., Lemmens M.A., Figueiredo-Pereira M.E. (2009) Subchronic infusion of the product of inflammation prostaglandin J2 models sporadic Parkinson's disease in mice J Neuroinflammation. Jul 25;6:18 Takeoka A. et al (2010) Noradrenergic innervation of the rat spinal cord caudal to a complete spinal cord transection: effects of olfactory ensheathing glia J Exp Neurol. 2010 Mar;222(1):59-69. Brown R.E. et al (2008) Characterization of GABAergic neurons in rapid-eye-movement sleep controlling regions of the brainstem reticular formation in GAD67-green fluorescent protein knock-in mice. Eur J Neurosci. 2008 Jan;27(2):352-63. Bisem NJ et al (2012) Mapping of FGF1 in the Medulla Oblongata of Macaca fascicularis. Acta Histochem Cytochem. 2012 Dec 26;45(6):325-34.
Specificity:
IHC on brain shows a pattern of staining specific for TH containing neurons. This antibody is known to react with rat, mouse and guinea pig. Cross reactivity with other species has not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Guinea Pig,Human,Pig,Rabbit,Rat
Immunogen:
A synthetic peptide (GLFSSYRLPGHTQDTLVAQKSS) as a part of porcine ChAT protein (aa: 167-188) conjugated to KLH
Applications:
ELISA,IHC-Frozen
Antibody Isotype:
IgG
Application Details:
IHC, 1 site ELISA. Use at a concentration of 1 µg/mL. This antiserum will superbly stain both cell bodies and nerve terminal. The optimal dilution should be determined by the end user.
Leong WK, Klaric TS, Lin Y, Lewis MD, Koblar SA.(2013) Upregulation of the neuronal Per-Arnt-Sim domain protein 4 (Npas4) in the rat corticolimbic system following focal cerebral ischemia. Eur J Neurosci. 2013 Feb 22. doi: 10.1111/ejn.12163. [Epub ahead of print] Application: IH; species Rat PubMed ID
Specificity:
This antibody stains cholinergic neurons in human, rat, guinea-pig and rabbit central and peripheral nervous systems. This antiserum is known to react with ChAT of origin: human, rat, guinea-pig and rabbit.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Sequestosome 1 Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Sequestosome 1 is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor and interleukin-1. It may regulate signaling cascades through ubiquitination and may also be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. It is present as a homooligomer or heterooligomer and localises to the cytoplasm, late endosome and nucleus. In cardiac muscles it localises to the sarcomeric band. It accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. It is also enriched in Rosenthal fibers of pilocytic astrocytoma and in liver cells it accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma. Sequestosome 1 is ubiquitously expressed and to date, there are 2 named isoforms produced by alternative splicing. Defects in sequestosome 1 cause sporadic and familial Page disease of bone (PDB). This is a metabolic bone disease characterised by focal areas of increased and disorganised bone turn-over due to activated osteoclasts.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Bovine,Human,Mouse,Rat
Immunogen:
Peptide IGAALDTIQYSKH corresponding to the C-terminal part of human Sequestosome 1 (aa: 424-436) conjugated to the Blue Carrier Protein has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:500 to 1: 5000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; ubiquitin-binding protein p62; EBI3-associated protein of 60 kDa; p60; EBIAP; SQSTM1; ORCA; OSIL
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity was confirmed by immunohistochemical analysis on paraffin embedded human brain. This antibody is expected to recognise p60 in mouse, rat and bovine.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Rabbit anti-Autophagy-related protein 7 (ATG7) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Homodimer (By similarity). Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12. SIMILARITY: Belongs to the ATG7 family. In yeast, ATG7 appears to be required for fusion of peroxisomal and vaculuolar membranes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (DSTRDRTLDQQC) corresponding to the C-terminal of human APG7 protein conjugated to blue carrier protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from APG7 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
Non-reduced western blot : 1:100; IF: acetone or 3.7% PFA fixed cells (HJ Lim et al 2013); 1:100; Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Chiu-Wei Chen, et al (2012) Inhibition of autophagy as a therapeutic strategy of iron-induced brain injury after hemorrhage Autophagy, 8(10):1510. Chien W.S. et al (2011) Suppression of autophagy in rat liver at late stage of polymicrobial sepsis Shock. 2011 Jan 14 Lee J.E. et al (2011) Autophagy Regulates Embryonic Survival During Delayed Implantation Endocrinology. 2011 Mar 1. Ryningen A et al (2012) Inhibition of Mammalian target of rapamycin in human acute myeloid leukemia cells has diverse effects that depend on the environmental in vitro stress. Bone Marrow Res. 2012;2012:329061. Oh HA et al (2013) Uncovering a role for endocannabinoid signaling in autophagy in preimplantation mouse embryos. Mol Hum Reprod. 2013 Feb;19(2):93-101.
Specificity:
IF, WB and ELISA confirmed the specificity for ATG7. Human, rat. Other species not yet tested.
Storage:
Store lyophilized antibody at 2-8ºC. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Presenilin 1 (PS-1) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Presenilin-1 (PSEN1) is a multi-pass membrane protein and component of the gamma-secretase complex. PSEN1 is thought to play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. It may also play a role in hematopoiesis. Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3), a familial early-onset form of Alzheimer disease (Ref:SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4. Contains no preservative.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a region (1-20 aa) from the N-terminus of human Presenilin 1 conjugated to Diptheria toxoid.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
IF and WB. Suggested dilution of 1:2,000 is recommended for WB. On SDS-PAGE, the predominant form detected by this antibody is the N-terminal Presenilin 1 fragment of approx 29 kDa. The uncleaved form of Presenilin 1 migrates to approx 45 kDa. Human and mouse brain samples commonly prepared with reducing agent (50mM DTT), urea (2.3M), SDS (1%) in 62.5 mM Tris-HCl pH 6.8 sample buffer heated to 50C for 15 min. The suggested dilution for IF is 1:100 for acetone or paraformaldehyde fixed cells or tissue. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Presenilin 1; PS-1; Protein S182; PS1-CTF12; PSEN1; AD3; PS1; PSNL1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity confirmed by WB and IF using transfected cells, Presenilin 1 knock-out mouse cells, mouse and human brain.
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Rabbit anti-Autophagy-related protein 9A (ATG9A) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Plays a role in autophagy. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein (Potential). ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. SIMILARITY: Belongs to the ATG9 family. IN YEAST: FUNCTION: Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Recruites ATG23 and ATG8 to the pre-autophagosomal structure. SUBUNIT: Interacts with ATG18, ATG2 and ATG23. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. Preautophagosomal structure; preautophagosomal structure membrane; multi-pass membrane protein. Note=Pre-autophagosomal and other perivacuolar punctate structures. The proper trafficking of ATG9 between the pre-autophagosomal structure and the other punctate structures requires ATG2, ATG18, ATG23, the ATG1-ATG13 complex and the phosphatidylinositol 3-kinase complex I. SIMILARITY: Belongs to the ATG9 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (CWHRRESDESGE) corresponding to the C-terminal of human APG9A (APG9L1) protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from APG9A (APG9L1) protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence, WB. A dilution of 1:100 to 1:3000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy-related protein 9A; APG9-like 1; Autophagy-related protein 9; APG9A; ATG9A; APG9L1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Park YE et al (2009) Autophagic degradation of nuclear components in mammalian cells. Autophagy. 2009 Aug;5(6):795-804.
Specificity:
IHC, WB and ELISA confirmed the specificity for ATG9A (APG9L1) . Human, rat. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Superoxide dismutase [Mn], mitochondrial destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems (Ref: SwissProt).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized serum with 0.02% thimerosal
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide (KHSLPDLPYDGALEPHINC) of human/rat/mouse mitochondrial manganese Superoxide Dismutase (SOD2), conjugated to Keyhole Limpet Hemocyanin.
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
IF, WB. Typical working dilutions for light microscopy are 1:500 to 1:1,000 depending on tissue and detection method. For IF, a dilution range of 1:50 to 1:100 is recommended. For WB, a dilution range of 1: 1,000 to 1: 4,000 is recommended. This antibody clearly detects a protein at approximately 24 kDa on WB of human brain tissue. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Superoxide dismutase [Mn], mitochondrial;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Mitochondrial manganese Superoxide Dismutase (SOD2), no cross reactivity to SOD1
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Peroxiredoxin-5 has a role in intracellular redox signaling.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized serum with 0.02% thimerosal
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (VKALNVEPDGTGLTC) corresponding to region (190-204 aa) of human Peroxiredoxin-5 conjugated to KLH. Human, mouse and rat sequences of this peptide are identical.
Applications:
ELISA,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, IF, WB. This antibody works in IHC on frozen or wax embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for light microscopy are 1:500 to 1:1,000 and IF 1:50 to 1:100. For WB a dilution range of 1: 1,000 to 1: 2,000 is recommended. This antibody clearly detects a protein at approximately 22 kDa on WB of human brain tissue. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human Peroxiredoxin-5 no cross reactivity to other family members
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Peroxiredoxin-4 has a probable role in redox regulation of the cell.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (SRVSVADHSLHLSKAKISK) corresponding to a region (65-83 aa) from human Peroxiredoxin-4 conjugated to diptheria toxin.
Applications:
ELISA,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB, ELISA. This antibody works in IHC on frozen or wax embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for light microscopy are 1:500 to 1:1,000 and IF of 1:50 to 1:100. For WB a dilution range of 1: 1,000 to 1: 4,000 is recommended. A dilution of 1: 1,000 to 1: 4,000 is recommended for ELISA. This antibody stains non-ciliated bronchiolar cells in rat lung and what appears to be sebaceous glands in rat skin. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Tam KC et al. (2018) Evidence for the interaction of peroxiredoxin-4 with the store-operated calcium channel activator STIM1 in liver cells. Cell Calcium. 2018 May 15;74:14-28.
Specificity:
Human Peroxiredoxin-4 no cross reactivity to other family members
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Peroxiredoxin-3 has a role in redox regulation of the cell.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized serum with 0.02% thimerosal
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (CEVVAVSVDSHFSHLAW) from a region (127-143 aa) on human Peroxiredoxin-3 conjugated to KLH.
Applications:
ELISA,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB, ELISA. This antibody works in IHC on frozen or wax embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for light microscopy are 1:500 to 1:1,000 and IF of 1:50 to 1:100. For WB a dilution range of 1: 1,000 to 1: 2,000 is recommended. This antibody detects a protein at approx 23 kDa on WB of human brain tissue which is the mature form of this protein. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Thioredoxin-dependent peroxide reductase, mitochondrial; Antioxidant protein 1; AOP-1; HBC189; Peroxiredoxin III; Prx-III; Peroxiredoxin-3; Protein MER5 homolog; PRDX3; AOP1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human Peroxiredoxin-3 no cross reactivity to other family members
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Peroxiredoxin-2 has a role in redox regulation of the cell.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized serum with 0.02% thimerosal
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (VVDGAFKEVKLS) corresponding to region (20-31 aa) from human Peroxiredoxin-2 conjugated to diptheria toxin.
Applications:
ELISA,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB, ELISA. This antibody works in IHC on frozen or wax embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for light microscopy are 1:500 to 1:1,000 and IF of 1:50 to 1:100. For WB a dilution range of 1: 1,000 to 1: 4,000 is recommended. A dilution of 1: 1,000 to 1: 4,000 is recommended for ELISA. This antibody stains basal cells in rat airways and specific kidney tubule cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human Peroxiredoxin-2, no cross reactivity to other family members
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Peroxiredoxin-1 has a role in redox regulation of the cell.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized serum with 0.02% thimerosal
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (PLVSDPKRTIAQDY) corresponding to the region 103-116 amino acids of human Peroxiredoxin-1 conjugated to diptheria toxin.
Applications:
ELISA,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB, ELISA. This antibody works in IHC on frozen or wax embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for light microscopy are 1:500 to 1:1,000 and IF of 1:50 to 1:100. For WB a dilution range of 1: 1,000 to 1: 4,000 is recommended. A dilution of 1: 1,000 to 1: 4,000 is recommended for ELISA. This antibody stains non-ciliated bronchiolar cells in rat airways and specific kidney tubule cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Rabbit anti-Autophagy-related protein 3 (ATG3) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Background Info:
FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. SIMILARITY: Belongs to the ATG3 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a central region of the human ATG3 protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from ATG3 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).
Applications:
IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB, immunofluorescence. A dilution of 1:50 to 1:3000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy-related protein 3; APG3; APG3-like; hApg3; Protein PC3-96 ; ATG3; APG3; APG3L
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Ryningen A et al (2012) Inhibition of Mammalian target of rapamycin in human acute myeloid leukemia cells has diverse effects that depend on the environmental in vitro stress. Bone Marrow Res. 2012;2012:329061.
Specificity:
IHC, WB and ELISA confirmed the specificity for ATG3. A 37 kDa band, that corresponds to the molecular weight of ATG3, is detected via western blot analysis. Human, rat, mouse. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Glutathione peroxidase 4 (GPx-4) is involved in protecting cells against membrane lipid peroxidation and cell death.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized serum with 0.02% thimerosal
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (VNYTQLVDLHARYAEC) corresponding to the amino acids 78-93 of human glutathione peroxidase 4 (Isoform Mitochondrial) conjugated to KLH has been used as the immunogen. Human, rat and mouse sequences are identical.
Applications:
IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
WB, IHC. Typical working dilution for IHC is 1:200 to 1:1,000 depending on tissue and detection method. For WB, a dilution range of 1:1,000 to 1:2,000 is recommended. GPx-4 exists as a tetramer and can reform into multimeric complexes even under reduced conditions. The reported molecular weight of the GPx-4 monomer is 22 kDa but is reported to rapidly form oligomers, thus higher molecular weight bands greater than 22 kDa are expected. Mitochondrial preparations are also recommended to enhance signal. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human and rat mitochondrial glutathione peroxidase (GPx-4) Human; rat;
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Glutathione peroxidase 1 has a role in detoxification of hydrogen peroxide and is one of the most important antioxidant enzymes in humans. It exists as a homotetramer which localises to the cytoplasm. It belongs to the glutathione peroxidase family. Glutathione peroxidase 1 is one of few proteins in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. This protein has a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine repeats. The allele with five alanine repeats is significantly associated with breast cancer risk. At least two alternatively spliced isoforms have been identified.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized serum with 0.02% thimerosal
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (RRYSRRFQTIDIEPDIEALL) corresponding to the amino acids 175-194 of human glutathione peroxidase 1 ( GPx-1) conjugated to diphtheria toxin has been used as the immunogen.
Applications:
ELISA,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB and ELISA. This antibody works superbly in IHC on frozen or wax embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine IHC are 1: 200 to 1: 1,000 depending on tissue and detection method. For WB a dilution range of 1: 1,000 to 1: 4,000 is recommended. For ELISA, a dilution of 1: 1,000 to 1: 4,000 is recommended. This antiserum has extremely high titre, it stains human and rat glial cells intensely and to some extent also stains neurons. Other tissues have not yet been tested. On WB under reducing conditions recognises a 22kD protein in human brain tissue and Sigma glutathione peroxidase (G-4013) isolated from human erythrocytes. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human and rat cellular selenium dependent glutathione peroxidase. Human; rat;
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles.
Rabbit anti-Autophagy-related protein 16-1 (ATG16 L1) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA, ICC.
Background Info:
ATG16 L1 plays an essential role in autophagy. It is a homooligomer ant interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). APG16 L1 localises to the cytoplasm, in particularly to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. There are 5 named isoforms produced by alternative splicing. Genetic variation within ATG16 L1 are associated with susceptibility to inflammatory bowel disease type 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
N-terminal of human ATG16-L1 protein conjugated to Blue Carrier Protein has been used as the immunogen.
Applications:
ELISA,ICC,WB
Antibody Isotype:
Mixed
Application Details:
Non-reduced western blot: 1:100; IP: 1-50-1:100 (5-10 ?L/500uL lysate); ELISA: 1:2000-1:3000 direct detection only, recombinant immunogen.
Alternative Names:
Autophagy-related protein 16-1; APG16-like 1; ATG16L1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Cooney R et al (2009). NOD2 stimulation induces autophagy in dendritic cells influencing bacterial handling and antigen presentation. Nat. Med. Dec 6 2009 Scharl M. et al (2011). Protein tyrosine phosphatase nonreceptor type 2 regulates autophagosome formation in human intestinal cells. Inflamm Bowel Dis. 2011 Oct 10.
Specificity:
Detects human protein via non-reduced WB, and ELISA Human, rat. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Protein painting of fourth Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Protein painting of fourth (Pof) is a probable RNA-binding protein that binds to the fourth chromosome and may bind a RNA that spreads the fourth chromosome (Ref: Swissprot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS (pH 7.4) with 0.02% Sodium azide added
Host Animal:
Rabbit
Species Reactivity:
Drosophila
Immunogen:
A synthetic peptide from Drosophila melanogaster Protein painting of fourth (22-36 aa) conjugated to KLH.
Applications:
ELISA
Antibody Isotype:
IgG
Application Details:
ELISA. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Zeste-interacting protein 16; Zip16; Pof;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Protein painting of fourth ;Zeste-interacting protein 16;
Storage:
Store lyophilized product at -20°C or below. After reconstitution, keep aliquots for 2-3 weeks at 2-8°C or at -20°C for up to 12 months. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Autophagy-related protein 4 homolog B (APG4B) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 5 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. SIMILARITY: Belongs to the peptidase C54 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (CLERFFDSEDED) corresponding to the C-terminus of human ATG4B protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from of ATG4B protein in mouse and rat.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence, WB. A dilution of 1:100 to 1:2000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A. J. Smuder et al (2011) Exercise protects against doxorubicin-induced markers of autophagy signaling in skeletal muscle. J Appl Physiol. 2011 Jul 21
Specificity:
IHC, WB and ELISA confirmed the specificity for ATG4B. Human, rat. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Neurturin (NTN) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Neurturin (NTN) is a member of the GDNF family of neurotrophic factors. This protein is a potent survival factor for several populations of central and peripheral neurons in mature and developing rodents. FUNCTION: Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. DISEASE: Defects in NRTN are a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, and possibly with other loci, defects in NRTN are involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human Neurturin (rh NTN)
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
Immunohistochemistry (IHC) at a suggested dilution of 1:2000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NRTN; NTN;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity of this product was demonstrated by immunohistochemistry and dot blot showed no cross reactivity with GDNF. This antibody is known to react with human, mouse and rat.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Autophagy-related protein 4 homolog C (ATG4C) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). SIMILARITY: Belongs to the peptidase C54 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (CTVYNSDVIDKQ) corresponding to the C-terminal of human ATG4C protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from ATG4C protein in mouse and rat.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence and WB. A dilution of 1:100 to 1:2000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Smuder AJ et al. (2011) Exercise protects against doxorubicin-induced markers of autophagy signaling in skeletal muscle. J Appl Physiol (1985). 2011 Oct;111(4):1190-8.
Specificity:
IHC, ELISA and WB confirmed the specificity for ATG4C. Human, rat. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
The elF2c (Argonaute, Ago) protein is a core protein of the RNA-induced silencing complex (RISC).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence (845-860 aa) from the C-terminus of mouse elF2C conjugated to KLH.
Applications:
IHC-Frozen,IHC-Paraffin-embedded,IP,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB), Immunochemistry (IHC) and Immunoprecipitation (IP). The recommended concentration for IHC in formalin fixed and paraffin embedded tissues and formalin/acetone fixed tissues is 1:200-1:500. For WB, the recommended concentration is 1:1,000 to 1:2,000. The elFC2 protein has a predicted length of 860 residues and the MW of the monomer is 94 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Protein argonaute-1; Protein argonaute-2; Protein argonaute-3; Argonaute1; Argonaute2; Argonaute3; mAgo1; mAgo2; Eukaryotic translation initiation factor 2C; eIF-2C 2; eIF-2C 1; eIF2C 2; Piwi/argonaute family protein meIF2C2 Protein slicer; Eif2c2; Ago1; Ago2;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Huang KL, Chadee AB, Chen CY, Zhang Y, Shyu AB. (2013)"Phosphorylation at intrinsically disordered regions of PAM2 motif-containing proteins modulates their interactions with PABPC1 and influences mRNA fate." RNA. 2013 Jan 22. [Epub ahead of print]. Lugli G. et al (2008) Expression of microRNAs and their precursors in synaptic fractions of adult mouse forebrain. J Neurochem. Jul;106(2):650-61. Lugli G. et al (2005) Dicer and eIF2c are enriched at postsynaptic densities in adult mouse brain and are modified by neuronal activity in a calpain-dependent manner. J Neurochem. Aug;94(4):896-905.
Specificity:
Specificity has been confirmed by IHC, WB and IP in mouse brain. This antibody is expected to recognise at least elF2c-1, 2 and 3 since they have identical C-termini. Human; mouse; rat; The Ago proteins are highly conserved so cross-reactivity with other species is expected.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Rabbit anti-Leptin Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Leptin is secreted by white adipocytes and functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. Leptin has a variety of other roles including endocrine functions, regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing (Ref Entrez Gene).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
Recombinant rat Leptin protein
Applications:
ELISA,WB
Antibody Isotype:
Mixed
Application Details:
WB and ELISA. Suggested dilution of 1:1,000-1:4,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Leptin; Obesity factor; Lep; Ob; Obese protein;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Mouse, Rat
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Rabbit anti-Leptin Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Leptin is secreted by white adipocytes and functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. Leptin has a variety of other roles including endocrine functions, regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing (Ref Entrez Gene).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant human Leptin protein
Applications:
ELISA,WB
Antibody Isotype:
Mixed
Application Details:
WB and ELISA. Suggested dilution of 1:1,000-1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Leptin; Obesity factor; Lep; Ob; Obese protein;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human Leptin
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Rabbit anti-Endothelin B receptor (ET-BR) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
The Endothelin B receptor is a multi-pass membrane protein that belongs to the G-protein coupled receptor 1 family. It acts as a non-specific receptor for endothelin 1, 2, and 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from human Endothelin B receptor (299-313 aa).
Applications:
WB
Antibody Isotype:
IgG
Application Details:
WB. Suggested dilution of 1:1,000-1:5,000. Human Endothelin B receptor has a predicted length of 442 residues and MW of 50 kDa. Biosensis recommends that the optimal working dilution should be determined by the end user.
Rabbit anti-Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3 B) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
MAP1LC3B is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. MAP1LC3B is probably involved in formation of autophagosomal vacuoles (autophagosomes). SUBCELLULAR LOCATION: LC3-I: Cytoplasm. LC3-II: Intracytoplasmic membrane; lipid-anchor. Cytoplasmic vesicle; autophagosome; autophagosomal membrane; lipid-anchor. LC3-II binds to the autophagic membranes. TISSUE SPECIFICITY: Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver. PTM: The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II. SIMILARITY: Belongs to the MAP1 LC3 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (FEQRVEDVRLIC) corresponding to the N-terminal of human MAP1LC3 B protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from mouse and rat MAP1LC3 B protein.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence, WB. A dilution of 1:100 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Theron AE et al. (2013) Molecular crosstalk between apoptosis and autophagy induced by a novel 2-methoxyestradiol analogue in cervical adenocarcinoma cells. Cancer Cell Int. 2013 Aug 27;13(1):87. Pilchowski R. et al. (2011) Specific Protein Patterns Characterize Metastatic Potential of Advanced Bladder Cancer. J Urol. 2011 Jun 16.
Specificity:
IHC, WB and ELISA confirmed the specificity for MAP1LC3 B. Human, rat. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Purification:
Whole serum
Target:
Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3 B)
Rabbit anti-Acetyl Lysine Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Lysine acetylation of histones and non-histone proteins plays an important part in many cellular processes such as chromatin and nuclear signaling, transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Rabbit
Species Reactivity:
Species Independent
Immunogen:
Acetylated Lysine OVA
Applications:
WB
Antibody Isotype:
IgG
Application Details:
WB. Suggested dilution of 1:1,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Acetyl-lysine;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Binds to proteins with acetylated lysine residues.
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Rabbit anti-Activity-regulated cytoskeleton-associated protein (Arg3.1) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Arc (also termed activity-regulated cytoskeleton-associated protein or Arg3.1), is an effector immediate early gene whose upregulation has been demonstrated during events of synaptic plasticity. Arg3.1 expression is detectable in neuronal cell bodies and dendrites in the brain regions including striatum and cortex hippocampus, hypothalamus, amygdala.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, containing no preservatives
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide from the C terminal of human Arg3.1 protein (ARC protein) conjugated to Blue Carrier Protein. The sequence is homologous with mouse and rat form of Arg3.1.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1: 50 to 1: 1000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specific for Arg3.1. Rat and Mouse. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability. Glycerol (1:1) may be added for an additional stability. Sodium azide or other appropriate antibacterial agent should be added if material is to be kept any length of time at 2-8°C. Avoid repetitive freeze/thaw cycles.
Purification:
Whole serum
Target:
Activity-regulated cytoskeleton-associated protein (Arg3.1)
Rabbit anti-Tyrosine Kinase Receptor A (TrkA) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, ELISA.
Background Info:
TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Cell membrane; single-pass type I membrane protein. Endocytosed to the endosomes upon treatment of cells with NGF. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. Both isoforms have similar biological properties. TISSUE SPECIFICITY: Isoform TrkA-II is primarily expressed in neuronal cells. Isoform TrkA-I is found in non-neuronal tissues. Mutations in TrkA have been associated with congenital insensitivity to pain, anhidrosis, self-mutalating behaviour, mental retardation and cancer.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Extracellular domain of glycosylated human TrkA protein produced in mammalian cells was used as the immunogen.
Applications:
ELISA,IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC (1:1000 dilution), Western Blotting (denaturing and <b>non-reducing</b> SDS-PAGE, 1:1000-1:2500 dilution), 1-site ELISA (1:5000 dilution). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Matusica D et al. (2020) Differentiation of the 50B11 dorsal root ganglion cells into NGF and GDNF responsive nociceptor subtypes. Mol Pain. 16:1744806920970368 Application: Rat, WB. Kobayashi K et al. (2015) Nerve Growth Factor Signals as Possible Pathogenic Biomarkers for Perineural Invasion in Adenoid Cystic Carcinom. Otolaryngol Head Neck Surg. 153(2):218-24 Application: Human, IHC. Uren RT et al. (2014) A novel role of suppressor of cytokine signaling-2 in the regulation of TrkA neurotrophin receptor biology. J Neurochem. 129(4):614-27 Application: Mouse, IF.
Specificity:
Specificity was demonstrated by immunohistochemistry. When used for immunohistochemistry in rat dorsal root ganglia, staining is restricted to the known distribution of TrkA, that is in small, nociceptive neurons. Reacts with human, rat and mouse TrkA. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Tyrosine Kinase Receptor C (TrkC) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
TrkC is a member of the neurotrophic tyrosine receptor kinase family. TrkC is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC is the receptor for neurotrophin-3 (NT-3). Signalling through TrkC leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 4 named isoforms produced by alternative splicing. Additional isoforms seem to exist. Mutations in TrkC have been associated with medulloblastomas, secretory breast carcinomas and other cancers.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Extracellular domain of glycosylated human TrkC protein produced in CHO cells was used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1:3000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity was demonstrated by immunohistochemistry. This antibody was used to stain cryostat sections of the rat peripheral sensory ganglia. Reacts with human, rat and mouse TrkC. Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Synthetic human Neurotensin peptide (151-163 aa) conjugated to BSA
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neuromedin N; NTS; NN; NmN; NT; NmN-125;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human; mouse; rat. Highly conserved and expected to interact with bovine, feline and monkey
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Rabbit anti-Somatostatin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Somatostatin is secreted by neuroendocrine neurons in hypothalamus and interacts with somatostatin receptors inhibiting secretion of somatotropin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic Somatostatin peptide (103-116) conjugated to BSA.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human Somatostatin. Cross reactivity is expected with mouse, rat, other species not yet tested.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for up to six months for a higher stability and at 2-8°C for one week with an appropriate antibacterial agent. Avoid freeze-thaw cycles
The hormone Atrial natriuretic peptide (ANP) is produced by cardiomyocites and is secreted into the bloodstream. ANP acts on convoluted tubules in kidney reducing sodium absorption which results in reducing the blood pressure.
Synthetic rat Atrial natriuretic peptide (123-150 aa) conjugated to BSA.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human; mouse; rat. Highly conserved and expected to interact with feline, canine, porcine, bovine, and equine ANP.
Storage:
At least 12 months after purchase at 2 - 8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for up to 6 months for a higher stability and at 2-8°C with an appropriate antibacterial agent for up to 2 weeks.Avoid freeze-thaw cycles
Rabbit anti-Sortilin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, ELISA.
Background Info:
FUNCTION: Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi. SUBUNIT: Interacts with LPL and SLC2A4. Interacts with the cytosolic adapter proteins GGA1 and GGA2. Interacts with numerous ligands including the receptor-associated protein LRPAP1/RAP, GM2A and PSAP. Forms a complex with NGFR which binds specifically to the precursor forms of NGFB (proNGFB) and BDNF (proBDNF). SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. Localized to membranes of the endoplasmic reticulum, endosomes, Golgi stack, lysosomes and nucleus. A small fraction of the protein is also localized to the plasma membrane. May also be found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. Localization to the plasma membrane in adipocytes may be enhanced by insulin. TISSUE SPECIFICITY: Expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta and testis. Expressed at lower levels in lymphoid organs, kidney, colon and liver. INDUCTION: During osteoblast differentiation. DOMAIN: The N-terminal propeptide may facilitate precursor transport within the Golgi stack. Intrachain binding of the N-terminal propeptide and the extracellular domain may also inhibit premature ligand binding. DOMAIN: The extracellular domain may be shed following protease cleavage in some cell types. PTM: The N-terminal propeptide is cleaved by furin and possibly other homologous proteases. PTM: Contains 8 intrachain disulfide bonds. PTM: N-glycosylated. SIMILARITY: Contains 9 BNR repeats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Extracellular domain of glycosylated human sortilin produced in CHO cells was used as the immunogen.
Applications:
ELISA,IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC, 1-site ELISA (1:5000 dilution). A dilution of 1:1000 is recommended for IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity was demonstrated by immunohistochemistry. Reacts with human, rat and mouse Sortilin. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Met-Enkephalin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Human Methionine enkephalin (Met-Enkephalin) is a small 5 amino acid peptide cleaved from the precursor pro-opiomelanocortin (POMC). Met-Enkephalin is an endogenous opioid peptide that interacts with opioid receptors and produces analgesic effects.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic human Met-enkephalin peptide (237-241) conjugated to BSA.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Pro-opiomelanocortin; POMC;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human; mouse; rat. Met-Enkephalin is highly conserved so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Leu-enkephalin 5.8%; with beta-lipotropin 0.01%; with beta-endorphin 0.01%
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Rabbit anti-Leu-Enkephalin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Leu-Enkephalin is cleaved from the precursor Proenkephalin-A. Leu-Enkephalin is an endogenous opioid peptide that interacts with opioid receptors and produces analgesic effects.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic human Leu-Enkephalin peptide (230 - 234) conjugated to BSA.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Proenkephalin-A; PENK;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human; mouse; rat. Leu-Enkephalin is highly conserved so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Met-enkephalin 0.93%; with beta-lipotropin 0.01%; with beta-endorphin 0.01%
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Rabbit anti-Gastrin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Human Gastrin is a 101 amino acid hormone produced by G cells of the duodenum, stomach and pancreas. It stimulates secretion of hydrochloric acid by parietal cells of the stomach. Gastrin is also secreted into bloodstream.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic human Gastrin peptide (76-85 aa) conjugated to BSA.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human Gastrin , Cross reactivity is expected with mouse, rat, other species not yet tested.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Endomorphins 1 and 2 are endogenous opioid peptides which have the highest affinity for the mu-opioid receptors. Located in various parts of the brain and interacts with mu-opioid receptors and produces analgesia.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic Endomorphin-2 (YPFF) peptide conjugated to BSA. Selectivity towards Endomorphin-2 was achieved by separating fraction of affinity purified antibodies which did not bind to Endomorphin-1.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
EM 2;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Endomorphin-2 peptide
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Rabbit anti-Endomorphin-1 and Endomorphin-2 (E-1/E-2) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Endomorphins 1 and 2 are endogenous opioid peptides which have the highest affinity for the mu-opioid receptors. Located in various parts of the brain and interacts with mu-opioid receptors and produces analgesia.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Antibodies to human Endomorphins 1 and 2 were made by immunising rabbits with the human Endomorphin-1 peptide (YPWF) and then selecting those antibodies that cross-reacted with both human Endomorphins 1 and 2.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
EM 1; EM 2;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human; mouse; rat. Endomorphin-1 is highly conserved molecule so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Endomorphin-2 = 70%; Met-enkephalin <0.03%; with Leu-enkephalin 0.01%; with beta-endorphin<0.1%.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Endomorphins 1 and 2 are endogenous opioid peptides which have the highest affinity for the mu-opioid receptors. Located in various parts of the brain and interacts with mu-opioid receptors and produces analgesia.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic human Endomorphin-1 (YPWF) peptide conjugated to BSA. Selectivity towards Endomorphin-1 was achieved by separating fraction of affinity purified antibodies which did not bind to Endomorphin-2.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
EM 1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human; mouse; rat. Endomorphin-1 is highly conserved so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Endomorphin-2 <5%; Met-enkephalin <0.03%; with Leu-enkephalin 0.01%; with beta-endorphin<0.1%
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Human beta-Lipotropin is a 93 amino acid polypeptide that is cleaved from carboxy-terminal fragment of the precursor pro-opiomelanocortin (POMC). It stimulates melanocytes to produce melanin, and can also be cleaved into smaller peptides including opioid peptides: gamma-lipotropin, alpha-MSH, beta-MSH, gamma-MSH, alpha-endorphin, beta-endorphin, gamma-endorphin and met-enkephalin
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic peptide (179-267 aa) of human beta-Lipotropin conjugated to thyroglobulin was used as the immunogen.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human; mouse; rat. Beta-Lipotropin is highly conserved so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Leu-enkephalin 0.01%; with Met-Enkephalin 0.01%; with beta-endorphin 0.01%
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Human beta-endorphin is a 31 amino acid peptide cleaved from the precursor pro-opiomelanocortin (POMC). It is an endogenous opioid peptide neurotransmitter that interacts with opioid receptors.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic human beta-endorphin peptide (1-31 aa) conjugated to BSA.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. For radioimmunoassay (RIA), a working dilution of 1:10,000 is recommended. Sensitivity - 120 pg/sample; IC50-1 ng/ sample. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
-endorphin; Pro-opiomelanocortin; POMC;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed by RIA against the synthetic peptide. Human; mouse; rat. Beta-endorphin is highly conserved so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Met-enkephalin 0.03%; with Leu-enkephalin 0.02%; with beta-lipotropin 0.34%.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2-IN-1), Purified Small Molecule, suitable to Block.
Background Info:
Leucine-Rich Repeat Kinase 2 (LRRK2) is a member of the leucine-rich repeat kinase family. Mutations in the LRRK2 gene have been linked with late-onset autosomal dominant Parkinson's disease (PD). A recent screening identified the analog LRRK2-IN-1 as a potent and selective inhibitor of LRRK2. This research by Deng et al (2011, Nat Chem Biol) showed that LRRK2-IN-1 suppressed LRRK2 kinase activity in vivo leading to dephosphorylation of Ser910/Ser935, loss of 14-3-3 binding and accumulation of LRRK2 within aggregrate fibrillar structures. LRRK2-IN-1, is the first selective and potent inhibitor of LRRK2. LRRK2-IN-1 inhibits both wild-type and G2019S mutant LRRK2 kinase activity with IC50 values of 13nM and 6nM respectively (Deng et al 2011, Nat Chem Biol).</div>
Product Type:
Small Molecule
Format:
Dry, Synthetic chemical powder
Applications:
Block
Application Details:
Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Deng X. et al (2011) Characterization of a selective inhibitor of the Parkinson's disease kinase LRRK2 Nat Chem Biol. 2011 Apr;7(4):203-5.
Storage:
Store dry, unopened vial protected from light for up to 3 months at RT or at -70ºC for up to 1 year. Reconstitute product in 100% ACS grade DMSO and store at -80ºC, in aliquots, protected from light, for up to 1 month. Prevent multiple freeze-thaw cycles. CAS Number 1234480-84-2.
Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2-IN-1), Purified Small Molecule, suitable to Block.
Background Info:
Leucine-Rich Repeat Kinase 2 (LRRK2) is a member of the leucine-rich repeat kinase family. Mutations in the LRRK2 gene have been linked with late-onset autosomal dominant Parkinson's disease (PD). A recent screening identified the analog LRRK2-IN-1 as a potent and selective inhibitor of LRRK2. This research by Deng et al (2011, Nat Chem Biol) showed that LRRK2-IN-1 suppressed LRRK2 kinase activity in vivo leading to dephosphorylation of Ser910/Ser935, loss of 14-3-3 binding and accumulation of LRRK2 within aggregrate fibrillar structures. LRRK2-IN-1, is the first selective and potent inhibitor of LRRK2. LRRK2-IN-1 inhibits both wild-type and G2019S mutant LRRK2 kinase activity with IC50 values of 13nM and 6nM respectively (Deng et al 2011, Nat Chem Biol).
Product Type:
Small Molecule
Format:
Dry, Synthetic chemical powder
Applications:
Block
Application Details:
Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Deng X. et al (2011) Characterization of a selective inhibitor of the Parkinson's disease kinase LRRK2 Nat Chem Biol. 2011 Apr;7(4):203-5.
Storage:
Store dry, unopened vial protected from light for up to 3 months at RT or at -70ºC for up to 1 year. Reconstitute product in 100% ACS grade DMSO and store at -80ºC, in aliquots, protected from light, for up to 1 month. Prevent multiple freeze-thaw cycles. CAS Number 1234480-84-2.
Alpha synuclein control peptide (116-131), Purified Peptide, suitable to Block.
Background Info:
<span itemprop="description">Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. Alpha synuclein has been associated with several neurodegenerative diseases. A point mutation in the gene coding for the alpha-synuclein protein was the first discovery linking this protein to a rare familial form of Parkinson's disease (PD). Subsequently, other mutations in the alpha-synuclein gene have been identified in familial PD. The aggregated proteinaceous inclusions called Lewy bodies found in PD and cortical Lewy body dementia (LBD) were discovered to be predominantly alpha-synuclein. Aberrant aggregation of alpha-synuclein has been detected in an increasing number of neurodegenerative diseases, collectively known as synucleopathies. Alpha-synuclein exists physiologically in both soluble and membrane-bound states, in unstructured and alpha-helical conformations, respectively. The physiological function of alpha-synuclein appears to require its translocation between these subcellular compartments and interconversion between the 2 conformations. Abnormal processing of alpha-synuclein is predicted to lead to pathological changes in its binding properties and function.</span>
Product Type:
Peptide
Format:
Lyophilized
Applications:
Block
Application Details:
Control Peptide. This peptide may be used to block the binding activity of antibodies to alpha synuclein ( see Biosensis antibodies S-024-100 and S-075-50).
Alternative Names:
Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
After reconstitution keep at -20°C. Avoid repetitive freeze/thaw cycles.
This peptide may be used to block binding activity of antibody to Parkin (# R-113-100).
Product Type:
Peptide
Format:
Lyophilized
Applications:
Block
Application Details:
Control peptide. This peptide may be used to block binding activity of antibody to Parkin (# R-113-100). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Ubiquitin E3 ligase PRKN; Parkinson juvenile disease protein 2; Parkinson disease protein 2; PARK2; PRKN
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
After reconstitution keep at -20°C. Avoid repetitive freeze/thaw cycles.
This peptide may be used to block binding activity of antibody to TrkB (# R-121-100).
Product Type:
Peptide
Format:
Lyophilized
Applications:
Block
Application Details:
Control peptide. This peptide may be used to block binding activity of antibody to TrkB (# R-121-100). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Tyrosine Kinase Receptor A (TrkA)-Fc Chimera, Purified Recombinant Protein, suitable to Block.
Background Info:
TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: TrkA is a heavily glycosylated transmembrane protein and contains 13 potential N-linked glycosylation sites. The TrkA-Fc chimera has N-linked and may have O-linked oligosaccharides.
Lyophilized product should be stored at 2 to 8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Tyrosine Kinase Receptor A (TrkA)-Fc Chimera, Purified Recombinant Protein, suitable to Block.
Background Info:
TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: TrkA is a heavily glycosylated transmembrane protein and contains 13 potential N-linked glycosylation sites. The TrkA-Fc chimera has N-linked and may have O-linked oligosaccharides.
Lyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
L-selectin-Fc Chimera, Human, Purified Recombinant Protein, suitable to Block.
Background Info:
L-selection (CD26L) is a cell surface adhesion protein constitutively expressed on all classes of human leukocytes. The L-Selectin glycoprotein is composed of a C-type lectin family domain at the amino terminus, an EGF-like domain, two short consensus repeat (SCR) sequences, a transmembrane domain and a short cytoplasmic tail. L-Selectin contains 7 potential N- linked glycosylation sites.
Product Type:
Protein
Format:
The L-Selectin-Fc chimera consists of 20-45% carbohydrate by weight. When reconstituted in 0.5 mL sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Lyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE and visualized by silver stain
L-selectin-Fc Chimera, Human, Purified Recombinant Protein, suitable to Block.
Background Info:
L-selection (CD26L) is a cell surface adhesion protein constitutively expressed on all classes of human leukocytes. The L-Selectin glycoprotein is composed of a C-type lectin family domain at the amino terminus, an EGF-like domain, two short consensus repeat (SCR) sequences, a transmembrane domain and a short cytoplasmic tail. L-Selectin contains 7 potential N- linked glycosylation sites.
Product Type:
Protein
Format:
The L-Selectin-Fc chimera consists of 20-45% carbohydrate by weight.
Lyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE and visualized by silver stain
L-selectin-Fc Chimera, Human, Purified Recombinant Protein, suitable to Block.
Background Info:
L-selection (CD26L) is a cell surface adhesion protein constitutively expressed on all classes of human leukocytes. The L-Selectin glycoprotein is composed of a C-type lectin family domain at the amino terminus, an EGF-like domain, two short consensus repeat (SCR) sequences, a transmembrane domain and a short cytoplasmic tail. L-Selectin contains 7 potential N- linked glycosylation sites.
Product Type:
Protein
Format:
The L-Selectin-Fc chimera consists of 20-45% carbohydrate by weight.
Lyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE and visualized by silver stain
Noggin, Human, Purified Recombinant Protein, suitable for Cell Culture.
Background Info:
Noggin is a glycoprotein predominantly expressed by the dorsal mesoderm during embryogenesis and is secreted as a covalently linked homodimer. Noggin is essential for cartilage morphogenesis and joint formation. It is an inhibitor of bone morphogenic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Defects in Noggin are a cause of symphalangism proximal syndrome (SYM1), of multiple synostoses syndrome 1 (SYNS1), of the tarsal-carpal coalition syndrome (TCC), of stapes ankylosis with broad thumb and toes, and of brachydactyly type B2 (BDB2).
Product Type:
Protein
Format:
The Noggin product consists of 5-25% carbohydrate by weight.
Applications:
Cell Culture
Alternative Names:
Nog;
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Lyophilized products should be stored at 2-8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95% as determined by SDS-PAGE and visualized by silver stain.
Noggin, Human, Purified Recombinant Protein, suitable for Cell Culture.
Background Info:
Noggin is a glycoprotein predominantly expressed by the dorsal mesoderm during embryogenesis and is secreted as a covalently linked homodimer. Noggin is essential for cartilage morphogenesis and joint formation. It is an inhibitor of bone morphogenic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Defects in Noggin are a cause of symphalangism proximal syndrome (SYM1), of multiple synostoses syndrome 1 (SYNS1), of the tarsal-carpal coalition syndrome (TCC), of stapes ankylosis with broad thumb and toes, and of brachydactyly type B2 (BDB2).
Product Type:
Protein
Format:
The Noggin product consists of 5-25% carbohydrate by weight.
Applications:
Cell Culture
Alternative Names:
Nog;
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Lyophilized products should be stored at 2-8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95% as determined by SDS-PAGE and visualized by silver stain.
Noggin, Human, Purified Recombinant Protein, suitable for Cell Culture.
Background Info:
Noggin is a glycoprotein predominantly expressed by the dorsal mesoderm during embryogenesis and is secreted as a covalently linked homodimer. Noggin is essential for cartilage morphogenesis and joint formation. It is an inhibitor of bone morphogenic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Defects in Noggin are a cause of symphalangism proximal syndrome (SYM1), of multiple synostoses syndrome 1 (SYNS1), of the tarsal-carpal coalition syndrome (TCC), of stapes ankylosis with broad thumb and toes, and of brachydactyly type B2 (BDB2).
Product Type:
Protein
Format:
The Noggin product consists of 5-25% carbohydrate by weight. When reconstituted in 0.5 mL sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Applications:
Cell Culture
Alternative Names:
Nog;
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Lyophilized products should be stored at 2-8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95% as determined by SDS-PAGE and visualized by silver stain.
Stem Cell Factor Receptor (SCFR) is a member of the receptor tyrosine kinase family. Binding of SCFR to its ligand Stem Cell Factor (PE-2008-5) induces receptor dimerization, autophosphorylation and signal transduction. SCFR is essential for the development of normal hematopoietic cells and plays an important role in the survival, proliferation and differentiation of mast cells, melanocytes and germ cells.
Product Type:
Protein
Format:
The SCFR-Fc Chimera consists of 5-45% carbohydrate by weight. When reconstituted in 0.5 mL sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 2-8°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE and visualized by silver stain
Stem Cell Factor Receptor (SCFR) is a member of the receptor tyrosine kinase family. Binding of SCFR to its ligand Stem Cell Factor (PE-1242-5) induces receptor dimerization, autophosphorylation and signal transduction. SCFR is essential for the development of normal hematopoietic cells and plays an important role in the survival, proliferation and differentiation of mast cells, melanocytes and germ cells.
Product Type:
Protein
Format:
Purified SCFR consists of approximately 15-45% carbohydrate by weight. When reconstituted in 0.5 mL sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Lyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 2-8°C and longer-term storage of aliquots at -18 to -20°C is recommended. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE, visualised by Coomassie Brilliant Blue
Stem Cell Factor Receptor (SCFR) is a member of the receptor tyrosine kinase family. Binding of SCFR to its ligand Stem Cell Factor (PE-1242-5) induces receptor dimerization, autophosphorylation and signal transduction. SCFR is essential for the development of normal hematopoietic cells and plays an important role in the survival, proliferation and differentiation of mast cells, melanocytes and germ cells.
Product Type:
Protein
Format:
Purified SCFR consists of approximately 15-45% carbohydrate by weight.
Lyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 2-8°C and longer-term storage of aliquots at -18 to -20°C is recommended. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE, visualised by Coomassie Brilliant Blue
Stem Cell Factor (SCF) is a hematopoietic growth factor that stimulates the proliferation of mast cells. SCF also mediates cell-cell adhesion and acts synergistically with other cytokines. The actions of SCF are exerted by binding to the SCF Receptor (PE-1243-50) which results in receptor dimerization. SCF is a type I membrane glycoprotein with 5 potential N-linked glycosylation sites. Soluble SCF is produced by proteolytic cleavage of the extracellular domain.
Product Type:
Protein
Format:
Purified SCF consists of 0-50% carbohydrate by weight. When reconstituted in 0.5 mL sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Lyophilized products should be stored at 2-8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE and visualized by silver stain
Stem Cell Factor (SCF) is a hematopoietic growth factor that stimulates the proliferation of mast cells. SCF also mediates cell-cell adhesion and acts synergistically with other cytokines. The actions of SCF are exerted by binding to the SCF Receptor (PE-1243-50) which results in receptor dimerization. SCF is a type I membrane glycoprotein with 5 potential N-linked glycosylation sites. Soluble SCF is produced by proteolytic cleavage of the extracellular domain.
Product Type:
Protein
Format:
Purified SCF consists of 0-50% carbohydrate by weight.
Lyophilized products should be stored at 2-8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE and visualized by silver stain
Hepatocyte growth factor (HGF) is a member of the plasminogen related growth factor family. The HGF protein is a dimer formed from an alpha and beta subunit linked by a disulfide bond. Both subunits originate from the endoproteolytic cleavage of a common precursor which is biologically inactive. HGF is expressed in Kupffer cella and sinusoidal endothelial cells in the liver and pancreas.
Product Type:
Protein
Format:
The HGF formulation consists of 0-30% carbohydrate by weight. When reconstituted in 0.5 mL sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 2-8°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE and visualised by Coomassie Brilliant Blue
Epidermal Growth Factor (EGF) Receptor binds to EGF and other members of the EGF family such as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. At least 4 isoforms are produced by alternative splicing (reference SWISSPROT). The EGF receptor is a single-pass type I membrane protein containing an extracellular receptor domain, a transmembrane region and an intracellular domain with tyrosine kinase function.
Product Type:
Protein
Format:
The EGF receptor-Fc chimera consists of 5-30% carbohydrate by weight. When reconstituted in 0.5 mL sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 2-8°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE and visualized by Coomassie Brilliant Blue
Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.
Product Type:
Protein
Format:
When reconstituted in 0.5 mL sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Applications:
Cell Culture
Alternative Names:
Beta-nerve growth factor; beta-NGF; NGFB;
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 2-8°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE and visualized by silver stain
Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.
Product Type:
Protein
Format:
Lyophilized, When reconstituted in 0.5 mL sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Applications:
Cell Culture
Alternative Names:
Beta-nerve growth factor; beta-NGF; NGFB;
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Lyophilized products should be stored at 2-8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE and visualized by silver stain
Nerve growth factor (NGF) receptor, also known as p75NTR, is a low affinity NGF receptor. It binds with equal affinity neurotrophins such as beta NGF, BDNF, NT-3 and NT-4. NGF receptors mediate signaling of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. These receptors are also thought to play a role in neurogenerative diseases such as Alzheimers disease. The NGF receptor is a type I transmembrane glycoprotein (399 aa) consisting of a signal peptide (28 aa), an extracellular domain (222 aa) which contains four cysteine rich domains responsible for ligand binding, a transmembrane domain (22 aa) and a cytoplasmic domain (155 aa).
Product Type:
Protein
Format:
The NGF Receptor-Fc chimera consists of 25-45% carbohydrate by weight.
Lyophilized products should be stored at 2-8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE and visualized by silver stain
Tyrosine Kinase Receptor B (TrkB)-Fc Chimera, Human, Purified Recombinant Protein, suitable to Block..
Background Info:
TrkC is a member of the tropomyosin-related kinase receptor family. TrkC is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC is the receptor for neurotrophin-3 (NT-3). Signalling through TrkC leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. TrkC is a heavily glycosylated molecule with 13 potential N-linked glycosylation sites within the extracellular domain.
Product Type:
Protein
Format:
The TrkC-Fc chimera consists of 15-55% carbohydrate by weight. When reconstituted in 0.5 mL sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 2-8°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE and visualized by silver stain.
Tyrosine Kinase Receptor B (TrkB)-Fc Chimera, Human, Purified Recombinant Protein, suitable for IP and to Block.
Background Info:
Tyrosine Kinase Receptor B (TrkB) is a high affinity receptor for brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5. It also binds neurotrophin 3 (NT-3) with lower affinity. TrkB does not bind nerve growth factor (NGF). TrkB is involved in the development and/or maintenance of the nervous system and is widely expressed, predominantly in nervous tissue. TrkB is a heavily glycosylated type I transmembrane protein. The TrkB-Fc chimera contains 13 potential N-linked glycosylation sites.
Product Type:
Protein
Format:
The chimera consists of 20-40% carbohydrate by weight.
Applications:
Block,IP
Application Details:
The TrkB-Fc chimera bound to protein A sepharose beads is able to pull down its ligand BDNF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Beros J et al. (2021) Age Related Response of Neonatal Rat Retinal Ganglion Cells to Reduced TrkB Signaling in vitro and in vivo. Front Cell Dev Biol. 9:671087. Application: Rat, Block. Beros J (2020) Pretreatment of ovaries with collagenase before vitrification keeps the ovarian reserve by maintaining cell-cell adhesion integrity in ovarian follicles. PhD Thesis. Application: Rat, Block.
Storage:
Lyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE and visualized by silver stain.
Tyrosine Kinase Receptor B (TrkB)-Fc Chimera, Human, Purified Recombinant Protein, suitable for IP and to Block.
Background Info:
Tyrosine Kinase Receptor B (TrkB) is a high affinity receptor for brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5. It also binds neurotrophin 3 (NT-3) with lower affinity. TrkB does not bind nerve growth factor (NGF). TrkB is involved in the development and/or maintenance of the nervous system and is widely expressed, predominantly in nervous tissue. TrkB is a heavily glycosylated type I transmembrane protein. The TrkB-Fc chimera contains 13 potential N-linked glycosylation sites.
Product Type:
Protein
Format:
The chimera consists of 20-40% carbohydrate by weight.
Applications:
Block,IP
Application Details:
The TrkB-Fc chimera bound to protein A sepharose beads is able to pull down its ligand BDNF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Beros J et al. (2021) Age Related Response of Neonatal Rat Retinal Ganglion Cells to Reduced TrkB Signaling in vitro and in vivo. Front Cell Dev Biol. 9:671087. Application: Rat, Block. Beros J (2020) Pretreatment of ovaries with collagenase before vitrification keeps the ovarian reserve by maintaining cell-cell adhesion integrity in ovarian follicles. PhD Thesis. Application: Rat, Block.
Storage:
Lyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Purification:
>95%, as determined by SDS-PAGE and visualized by silver stain.
Mouse NGF (2.5S) was isolated from mouse submaxillary glands by method of Mobley et al (1976) and is a form of beta-NGF that has identical biological properties. NGF is known to regulate the survival and development of certain sympathetic and sensory neurons. It is a dimer with 2 identical polypeptide chains and dimeric molecular weight of approximately 26,500 Da. Isolation and purification of NGF from mouse submaxillary glands yields preparations of NGF (2.5S) with identical biological activity but with cleavages at the amino terminus (with the loss of 8 amino acids) and/or at the carboxy-terminus (with the loss of arginine). These preparations are named 2.5 NGF (see reference below).
Background Info:
Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.
Product Type:
Protein
Format:
Lyophilized from PBS, pH 7.4 without preservatives.
Applications:
Cell Culture,ELISA,WB
Application Details:
Stimulates neurite outgrowth in rat PC12 cells
Alternative Names:
mouse NGF; beta NGF
Biological Activity:
Stimulates neurite outgrowth in rat PC12 cells
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt for lyophilized material. Reconstituted material, 5 days at 4C, 30 days -20°C, 60 days -70°C for highest activity Avoid repeated freezing and thawing. Use insulated storage containers for best results.
Use:
For research use only.
Product references:
Wang YN et al (2021) Slit3 secreted from M2-like macrophages increases sympathetic activity and thermogenesis in adipose tissue. Nat Metab. 3(11):1536-1551. Walters RR et al (2021) Pharmacokinetics and Immunogenicity of Frunevetmab in Osteoarthritic Cats Following Intravenous and Subcutaneous Administration. Front Vet Sci. 8:687448. Carter DR et al (2016) Glutathione biosynthesis is upregulated at the initiation of MYCN-driven neuroblastoma tumorigenesis. Mol Oncol. 2016 Jun;10(6):866-78. Goodhill GJ et al (2015) The dynamics of growth cone morphology. BMC Biol. 2015 Feb;13(10). Coulson EJ et al (2015) Neurotrophin-tyrosine kinase receptor signaling. United States Patent Application 20150344535. Gearing, DP et al (2013) A fully caninised anti-NGF monoclonal antibody for pain relief in dogs. BMC Vet Res. 9:226. Matusica D et al (2013) An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function. J Biol Chem. 2013 Apr 19;288(16):11144-54. Yuan J et al (2013) Optimality and saturation in axonal chemotaxis. Neural Comput. 2013 Apr;25(4):833-53. Sykes AM et al (2012) The effects of transmembrane sequence and dimerization on cleavage of the p75 neurotrophin receptor by gamma-secretase. J Biol Chem. 2012 Dec 21;287(52):43810-24. E.M. Forbes et al (2012) Calcium and cAMP Levels Interact to Determine Attraction versus Repulsion in Axon Guidance. Neuron. 2012 May 10;74(3):490-503.
Storage:
Store lyophilized protein at 2-8°C. After reconstitution, store at 2-8°C short term. Store long-term at -20°C to -80°C. Avoid repeated freezing and thawing. See expiration date for shelf-life estimates, actual times may vary depending upon experimental conditions and laboratory handling.
Purification:
Greater than 90% (as determined by SDS electrophoresis)
Mouse NGF (2.5S) was isolated from mouse submaxillary glands by method of Mobley et al (1976) and is a form of beta-NGF that has identical biological properties. NGF is known to regulate the survival and development of certain sympathetic and sensory neurons. It is a dimer with 2 identical polypeptide chains and dimeric molecular weight of approximately 26,500 Da. Isolation and purification of NGF from mouse submaxillary glands yields preparations of NGF (2.5S) with identical biological activity but with cleavages at the amino terminus (with the loss of 8 amino acids) and/or at the carboxy-terminus (with the loss of arginine). These preparations are named 2.5 NGF (see reference below).
Background Info:
Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.
Product Type:
Protein
Format:
Lyophilized from PBS, pH 7.4 without preservatives.
Applications:
Cell Culture,ELISA,WB
Application Details:
Stimulates neurite outgrowth in rat PC12 cells
Alternative Names:
mouse NGF; beta NGF
Biological Activity:
Stimulates neurite outgrowth in rat PC12 cells
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt for lyophilized material. Reconstituted material, 5 days at 4C, 30 days -20°C, 60 days -70°C for highest activity Avoid repeated freezing and thawing. Use insulated storage containers for best results.
Use:
For research use only.
Product references:
Wang YN et al (2021) Slit3 secreted from M2-like macrophages increases sympathetic activity and thermogenesis in adipose tissue. Nat Metab. 3(11):1536-1551. Walters RR et al (2021) Pharmacokinetics and Immunogenicity of Frunevetmab in Osteoarthritic Cats Following Intravenous and Subcutaneous Administration. Front Vet Sci. 8:687448. Carter DR et al (2016) Glutathione biosynthesis is upregulated at the initiation of MYCN-driven neuroblastoma tumorigenesis. Mol Oncol. 2016 Jun;10(6):866-78. Goodhill GJ et al (2015) The dynamics of growth cone morphology. BMC Biol. 2015 Feb;13(10). Coulson EJ et al (2015) Neurotrophin-tyrosine kinase receptor signaling. United States Patent Application 20150344535. Gearing, DP et al (2013) A fully caninised anti-NGF monoclonal antibody for pain relief in dogs. BMC Vet Res. 9:226. Matusica D et al (2013) An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function. J Biol Chem. 2013 Apr 19;288(16):11144-54. Yuan J et al (2013) Optimality and saturation in axonal chemotaxis. Neural Comput. 2013 Apr;25(4):833-53. Sykes AM et al (2012) The effects of transmembrane sequence and dimerization on cleavage of the p75 neurotrophin receptor by gamma-secretase. J Biol Chem. 2012 Dec 21;287(52):43810-24. E.M. Forbes et al (2012) Calcium and cAMP Levels Interact to Determine Attraction versus Repulsion in Axon Guidance. Neuron. 2012 May 10;74(3):490-503.
Storage:
Store lyophilized protein at 2-8°C. After reconstitution, store at 2-8°C short term. Store long-term at -20°C to -80°C. Avoid repeated freezing and thawing. See expiration date for shelf-life estimates, actual times may vary depending upon experimental conditions and laboratory handling.
Purification:
Greater than 90% (as determined by SDS electrophoresis)
Mouse NGF (2.5S) was isolated from mouse submaxillary glands by method of Mobley et al (1976) and is a form of beta-NGF that has identical biological properties. NGF is known to regulate the survival and development of certain sympathetic and sensory neurons. It is a dimer with 2 identical polypeptide chains and dimeric molecular weight of approximately 26,500 Da. Isolation and purification of NGF from mouse submaxillary glands yields preparations of NGF (2.5S) with identical biological activity but with cleavages at the amino terminus (with the loss of 8 amino acids) and/or at the carboxy-terminus (with the loss of arginine). These preparations are named 2.5 NGF (see reference below).
Background Info:
Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.
Product Type:
Protein
Format:
Lyophilized from PBS, pH 7.4 without preservatives.
Applications:
Cell Culture,ELISA,WB
Application Details:
Stimulates neurite outgrowth in rat PC12 cells
Alternative Names:
mouse NGF; beta NGF
Biological Activity:
Stimulates neurite outgrowth in rat PC12 cells
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt for lyophilized material. Reconstituted material, 5 days at 4C, 30 days -20°C, 60 days -70°C for highest activity Avoid repeated freezing and thawing. Use insulated storage containers for best results.
Use:
For research use only.
Product references:
Wang YN et al (2021) Slit3 secreted from M2-like macrophages increases sympathetic activity and thermogenesis in adipose tissue. Nat Metab. 3(11):1536-1551. Walters RR et al (2021) Pharmacokinetics and Immunogenicity of Frunevetmab in Osteoarthritic Cats Following Intravenous and Subcutaneous Administration. Front Vet Sci. 8:687448. Carter DR et al (2016) Glutathione biosynthesis is upregulated at the initiation of MYCN-driven neuroblastoma tumorigenesis. Mol Oncol. 2016 Jun;10(6):866-78. Goodhill GJ et al (2015) The dynamics of growth cone morphology. BMC Biol. 2015 Feb;13(10). Coulson EJ et al (2015) Neurotrophin-tyrosine kinase receptor signaling. United States Patent Application 20150344535. Gearing, DP et al (2013) A fully caninised anti-NGF monoclonal antibody for pain relief in dogs. BMC Vet Res. 9:226. Matusica D et al (2013) An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function. J Biol Chem. 2013 Apr 19;288(16):11144-54. Yuan J et al (2013) Optimality and saturation in axonal chemotaxis. Neural Comput. 2013 Apr;25(4):833-53. Sykes AM et al (2012) The effects of transmembrane sequence and dimerization on cleavage of the p75 neurotrophin receptor by gamma-secretase. J Biol Chem. 2012 Dec 21;287(52):43810-24. E.M. Forbes et al (2012) Calcium and cAMP Levels Interact to Determine Attraction versus Repulsion in Axon Guidance. Neuron. 2012 May 10;74(3):490-503.
Storage:
Store lyophilized protein at 2-8°C. After reconstitution, store at 2-8°C short term. Store long-term at -20°C to -80°C. Avoid repeated freezing and thawing. See expiration date for shelf-life estimates, actual times may vary depending upon experimental conditions and laboratory handling.
Purification:
Greater than 90% (as determined by SDS electrophoresis)
CNTF is a survival promoting factor for different types of neurons in vitro and in vivo. The essential structural features for the biological function of human CNTF were investigated by Thier, M. et al. They showed that deletion of 14 N-terminal and 18 C-terminal amino acids significantly increased bioactivity compared to wild-type CNTF. FUNCTION: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Nervous system. PHARMACEUTICAL: CNTF is being tested under the name Axokine by Regeneron Pharmaceuticals for treatment of human motor neuron diseases, such as amyotrophic lateral sclerosis (ALS). As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. SIMILARITY: Belongs to the CNTF family.
Product Type:
Protein
Format:
Lyophilized
Applications:
Cell Culture
Alternative Names:
Ciliary NeuroTrophic Factor
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Qiping D et al. (2018) Mechanism and consequence of abnormal calcium homeostasis in Rett syndrome astrocytes. eLife. [Accepted Manuscript]. Williams EC et al. (2014) Mutant astrocytes differentiated from Rett syndrome patients-specific iPSCs have adverse effects on wild-type neurons. Hum Mol Genet. 2014 Jun 1;23(11):2968-80.
Storage:
Aliquot and keep at -20°C for long-term storage. For short term keep at 2-8°C. Avoid repetitive freeze/thaw cycles.
Ribosome-inactivating Purified Protein saporin-6 (Saporin), Purified Native Purified Protein, suitable for WB.
Background Info:
Saporin is a ribosome-inactivating protein (RIP) of type I. This monomeric RNA N-glycosidase purified from seeds of the plant Saponaria officinalis also known as Soapwort, is capable of specific depurination of eukaryotic ribosomes thus arresting protein synthesis. No ligand has been identified in saporin hence its inability to transverse the cell membrane. Due to its toxicity and stability of the structure, saporin has proven extremely useful for construction of immunotoxins. Biosensis Saporin is purified in-house and available in bulk reuqest upon request as well.
Product Type:
Protein
Format:
Lyophilized
Applications:
WB
Alternative Names:
Saponaria officinalis; Common soapwort; ribosome inactivating protein saporin-6; SAP-6; SO-6; rRNA N-glycosidase
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Keep lyophilized protein at 2-8ºC. Aliquot and keep at -20°C for long-term storage. For short term keep aliquots at 2-8ºC. Avoid repetitive freeze/thaw cycles.
Purification:
> 95% by SDS-PAGE. Purified from seeds of the plant Saponaria officinalis.
Mouse anti-NeuN/Fox3 Monoclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
Background Info:
Fox3 is one of a family of mammalian homologues of Fox-1. The Fox proteins are about 46 kDa in size, and each includes a central highly conserved RRM type RNA recognition motif. Much interest has focused on Fox3 as a result of the recent finding that this protein corresponds to NeuN, a neuronal nuclear antigen. NeuN/Fox-3 has a function in RNA splicing and is expressed heavily and specifically in neuronal nuclei and cytoplasm. Our antibody was raised against the N-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli. We did not use full length Fox3 as immunogen since the three mammalian Fox homologues, namely Fox1, Fox2 and Fox3, include virtually identical RRM motifs. The N-terminal region of the three molecules are much more variable in the three molecules so antibodies specific for each of the three molecules can therefore be generated.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
Antibody was raised against the N-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli.
Applications:
ICC,IHC-Frozen,WB
Clone number:
1B7
Antibody Isotype:
IgG2a
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:1,000 - 1:2,000 is recommended for WB, ICC and IHC (frozen sections). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Feminizing locus on X; Fox-1; Fox3; NeuN;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Suzuki Y et al. (2022) "High-Throughput Screening Assay Identifies Berberine and Mubritinibas Neuroprotection Drugs for Spinal Cord Injury via Blood?Spinal Cord Barrier Protection." Neurotherapeutics. [Epub ahead of print] Application: IHC (IF). Species: Mouse. Yeh SJ et al. (2021) "Capping Protein Regulator and Myosin 1 Linker 3 (CARMIL3) as a Molecular Signature of Ischemic Neurons in the DWI-T2 Mismatch Areas After Stroke." Front Mol Neurosci. 14:754762. Application: IHC (IF). Species: Rat. Han Y & Zhou XF (2019) "Method of Producing Multipotent Stem Cells." US Patent US 10,196,606 B2 . Application: ICC (IF). Species: Human. Santos J et al. (2017) "Proteomic Analysis of Human Adipose Derived Stem Cells during Small Molecule Chemical Stimulated Pre-neuronal Differentiation." Int J Stem Cells. 2017; 10(2):193-217. Application: WB. Species: Human. Hamanoue M et al. (2016) "Cell-permeable p38 MAP kinase promotes migration of adult neural stem/progenitor cells" Sci Rep. 6:24279. Application: WB. Species: Mouse. Han YC et al. (2016) "Direct Reprogramming of Mouse Fibroblasts to Neural Stem Cells by Small Molecules" Stem Cells Int. 2016; 2016:4304916. Application: ICC (IF). Species: Mouse.
Specificity:
The specificity of this antibody has been confirmed by WB. Two alternate transcripts can be seen at 46 kDa and 48 kDa.
Storage:
Store lyophilized antibody at 2-8ºC for up to 12 months after date of receipt. After reconstitution of lyophilized antibody, aliquot and store at -20°C for up to 6 months for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Neurofilament light (NF-L) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC, FC.
Background Info:
Neurofilaments are composed of three intermediate filament proteins: light (NF-L ~68 kDa), medium (NF-M ~160 kDa) and heavy (NF-H ~200 kDa), found specifically in neurons, which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
C-terminal peptide of human NF-L protein (amino acids 514-542). The antibody has been made against the peptide GEEEDTKESEEEEKKEESAGEEQVAKKKD with an N-terminal C added for coupling to KLH.
Applications:
FC,ICC,IHC-Frozen,WB
Clone number:
6H112
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) and Flow Cytometry (2 ug per 10^6 cells). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:100 - 1:500 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Mouse anti-Neurofilament light (NF-L) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC, FC.
Background Info:
Neurofilaments are composed of three intermediate filament proteins: light (NF-L ~68 kDa), medium (NF-M ~160 kDa) and heavy (NF-H ~200 kDa), found specifically in neurons, which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
Full length native protein purified from pig spinal cord. The antibody binds NF-L from a variety of species including human, rat and mouse.
Applications:
FC,ICC,IHC-Frozen,WB
Clone number:
7D1
Antibody Isotype:
IgG2b
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) and Flow Cytometry (2 ug per 10^6 cells). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:100 - 1:500 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Mouse anti-Beta-synuclein Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease (Ref: uniprot.org).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
C-terminal peptide of human ?-synuclein (EPEGESYEDPPQEEYQEYEPEA) coupled to KLH.
Applications:
IHC-Frozen,WB
Clone number:
6A10
Antibody Isotype:
IgG1
Application Details:
Western blotting (1:1,000) and Immunohistochemistry (frozen sections, 1:500-1:1,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-synuclein, ?-synuclein
Biosensis Brand:
Biosensis®
Cellular Localisation:
Intracellular, cytosolic.
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Immunogen epitope location:
C-terminus.
Immunogen length:
22 amino acids.
Physical State:
Solid.
Specificity:
Specific for ?-synuclein, does not cross-react with ?- or ?-synuclein.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Product Validation Info:
Validated by western blotting and immunohistochemical procedures.
Purification:
Affinity-purified from conditioned medium using the immunogen.
Mouse anti-Green fluorescent protein (GFP) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
The green fluorescent protein (GFP) is a 27 kDa protein isolated originally from the jellyfish Aequoria victoria. It has an endogenous fluorochrome activity with excitation maximum at 395 nm and emission maximum at 509 nm, which is similar to that of fluorescein. GFP can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell.<br> This GFP rabbit antibody was made against a recombinant GFP construct originating from an Aequoria species which was engineered to improve spectral properties and prevent oligomerization. This form of GFP, referred to as AcGFP, is 94% identical to the eGFP developed by Tsien and co-workers. The antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Species Independent
Immunogen:
Recombinant AcGFP protein expressed in and purified from E. Coli.
Applications:
ICC,WB
Clone number:
1F1
Antibody Isotype:
IgM
Application Details:
Western blotting (1:1,000) and Immunocytochemistry (1:1,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Green fluorescent protein, GFP
Biosensis Brand:
Biosensis®
Cellular Localisation:
Intracellular, cytosolic.
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Immunogen epitope location:
The epitope is in the N-terminal 18 amino acids of the protein (peptide MVSKGAELFTGIVPILIE), which is found in the Clontech and other GFP vectors
Immunogen length:
Full-length recombinant protein.
Negative Control:
Non-transfected HEK293 cells.
Physical State:
Solid.
Positive Control:
GFP-transfected HEK293 cells.
Specificity:
Specific for GFP, does not cross-react with mCherry.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Product Validation Info:
Antibody recognizes GFP protein in GFP-transfected HEK293 cells, but not in non-transfected control cells.
Mouse anti-Tyrosine Kinase Receptor C (TrkC) Monoclonal Antibody (Unconjugated), suitable for FC, ICC.
Background Info:
Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation (Ref: uniprot.org).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS, pH 7.2-7.6 with 0.1% trehalose and contains no preservatives.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic peptide immunogen, KEPFPESTDNFI, cooresponding to amino acids 397-408 of human TrkC ECD.
Applications:
FC,ICC
Clone number:
BS337
Antibody Isotype:
IgG2b, kappa
Application Details:
Flow Cytometry (5-10 µg/mL): Tested on human and rodent cell lines. Cell staining can be performed under native conditions on ice, or on fixed cells with up to 4% formaldehyde. Immunocytochemistry (1-2 µg/mL): Tested on fixed (4% formaldehyde) human cells. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human TrkC ECD, cross reactivity to rodent species is expected.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Purification:
Protein G purified immunoglobulin from tissue culture supernatants.
Mouse anti-Tyrosine Kinase Receptor B (TrkB) Monoclonal Antibody (Unconjugated), suitable for FC.
Background Info:
The protein named TrkB (also named Neurotrophic tyrosine kinase receptor type 2 (NTRK2), GP145-TrkB or Tropomyosin-related kinase B is a receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems and is important in the regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. TrkB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. TrkB is the primary receptor for BDNF (brain-derived neurotrophic factor. TrkB also binds NT4 and NT3 but less efficiently. Upon ligand-binding, the receptor undergoes homodimerization, autophosphorylation and activation. TrkB activation recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that each regulate distinct overlapping signaling cascades within cells. Through SHC1, FRS2, SH2B1, SH2B2, these activate the GRB2-Ras-MAPK cascade that regulates, for instance, neuronal differentiation including neurite outgrowth. These same effectors also control the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. TrkB, via activation of PLCG1 and the downstream protein kinase C-regulated pathways, also controls synaptic plasticity, and thus plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. One such consequence is that PLCG1 activation via TrkB is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. (Reference: www.uniprot.org)
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS, pH 7.2-7.6 with 0.1% trehalose.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic peptide immunogen, ESSKNIPLANLQ. Extracellular domain of TrkB (amino acids 179-190 of human TrkB).
Applications:
FC
Clone number:
BS379
Antibody Isotype:
IgG2b, kappa
Application Details:
Flow Cytometry (5-10 µg/mL): Tested on human and rodent cell lines. Cell staining can be performed under native conditions on ice, or on fixed cells with up to 4% formaldehyde. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TrkB; detects TrkB in both human, mouse and rat samples by Flow cytometry
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Mouse anti-Glial Fibrillary Acidic Protein (GFAP) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
GFAP isolated biochemically from pig spinal cord was used as the immunogen.
Applications:
IHC-Frozen,WB
Clone number:
2A5
Antibody Isotype:
IgG1
Application Details:
Western Blot (1:1,000-1:2,000): tested on rat, mouse brain and spinal cord, human recombinant protein, pig brain. Immunohistochemistry (1:500-1:1,000): tested on rat cerebellum section. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Glial fibrillary acidic protein; GFAP
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody is specific for GFAP as demonstrated by western blotting and immunohistochemistry.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
p75NTR (CD271) was originally discovered as a low affinity nerve growth factor receptor (NGFR). Later it was found that it was the receptor for all neurotrophins, including NGF, BDNF, NT3 and NT4/5. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. Recent research proposes the extracellular domain of p75NTR as a biomarker for monitoring the progression of motor neuron disease (MND), also known as Amyotrophic Lateral Sclerosis (ALS) or Lou Gehrig's Disease. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid antibody (1 mg/mL) in PBS, pH 7.2-7.6, without preservative. Typical Fluorophore/Protein (F/P) - ratio is 3-10.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.
Applications:
FC,ICC
Clone number:
8J2
Antibody Isotype:
IgG2a
Application Details:
Immunocytochemistry: 1-5 µg/mL. Light fixation only, or unfixed works best. Epitope is sensitive to fixation.<br>Flow Cytometry: 5-20 µg/mL.<br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human, reacts with human, mouse and rat. Cross-reactivity with other species not tested but expected.This antibody is specific for NGFR/p75NTR as demonstRated by western blotting and immunprecipitation. The antibody recognizes extracellular p75NTR under non-reducing conditions.
Storage:
Liquid antibody is shipped cold. Upon arrival, spin vial briefly, divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Purification:
Protein A purified IgG was labelled with ATTO 488 and free dye removed by gel filtration.
p75NTR (CD271) was originally discovered as a low affinity nerve growth factor receptor (NGFR). Later it was found that it was the receptor for all neurotrophins, including NGF, BDNF, NT3 and NT4/5. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. Recent research proposes the extracellular domain of p75NTR as a biomarker for monitoring the progression of motor neuron disease (MND), also known as Amyotrophic Lateral Sclerosis (ALS) or Lou Gehrig's Disease. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid antibody (1 mg/mL) in PBS, pH 7.2-7.6, without preservative. Typical Fluorophore/Protein (F/P) - ratio is 3-10.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.
Applications:
FC,ICC
Clone number:
8J2
Antibody Isotype:
IgG2a
Application Details:
Immunocytochemistry: 1-5 µg/mL. Light fixation only, or unfixed works best. Epitope is sensitive to fixation.<br>Flow Cytometry: 5-20 µg/mL.<br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human, reacts with human, mouse and rat. Cross-reactivity with other species not tested but expected.This antibody is specific for NGFR/p75NTR as demonstRated by western blotting and immunprecipitation. The antibody recognizes extracellular p75NTR under non-reducing conditions.
Storage:
Liquid antibody is shipped cold. Upon arrival, spin vial briefly, divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Purification:
Protein A purified IgG was labelled with FITC and free dye removed by gel filtration.
p75NTR (CD271) was originally discovered as a low affinity nerve growth factor receptor (NGFR). Later it was found that it was the receptor for all neurotrophins, including NGF, BDNF, NT3 and NT4/5. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. Recent research proposes the extracellular domain of p75NTR as a biomarker for monitoring the progression of motor neuron disease (MND), also known as Amyotrophic Lateral Sclerosis (ALS) or Lou Gehrig's Disease. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.
Applications:
FC,ICC,IHC-Frozen,Immunopanning,IP,WB
Clone number:
8J2
Antibody Isotype:
IgG2a
Application Details:
Flow Cytometry: 5-20 µg/mL.<br>Western Blotting: 0.5-2.0 µg/mL, <b>non-reducing conditions only</b> (no DTT or beta-mercaptoethanol).<br>Immunoprecipitation: lysate dependent. 10 ug per 200-500 ug total protein.<br>Immunopanning: 1-5 µg/mL.<br>Immunocytochemistry: 1-5 µg/mL. Staining is strongest in non-fixed cells, light fixation is tolerable.<br>Immunohistochemistry: fresh, acetone fixed sections only, epitope is fixation sensitive. Not suitable in formalin-fixed, paraffin (FFPE) embedded tissues.<br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Hulme AJ et al. (2020) Molecular and Functional Characterization of Neurogenin-2 Induced Human Sensory Neurons . Front Cell Neurosci. 14:600895 Application: Human, ICC(IF).
Specificity:
Human, reacts with human, mouse and rat. Cross-reactivity with other species not tested but expected.This antibody is specific for NGFR/p75NTR as demonstRated by western blotting and immunprecipitation. The antibody recognizes extracellular p75NTR under non-reducing conditions.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Mouse anti-Rhodopsin Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth (By similarity). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins (PubMed:10926528, PubMed:12044163, PubMed:11972040, PubMed:16908857, PubMed:16586416, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:1396673, PubMed:15111114). Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
Purified bovine rhodopsin
Applications:
IHC-Frozen,WB
Clone number:
B630
Antibody Isotype:
IgG1
Application Details:
Western blotting (1:5,000) and Immunohistochemistry (1:1,000). Due to the highly hydrophobic nature of rhodopsin, avoid boiling samples in SDS-PAGE sample buffer for rhodopsin analysis by Western Blotting, as this will result in extensive aggregation of the rhodopsin protein and appearance of high molecular weight bands. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Bovine,reacts with Human, Rat, Mouse, Cow, Pig. Expected to react with other mammalian species.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Mouse anti-Parvalbumin Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Full-length recombinant human protein
Applications:
IHC-Frozen,WB
Clone number:
3C9
Antibody Isotype:
IgG1
Application Details:
Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000-1:5,000). Note that this antibody does not recognize parvalbumin in rat or mouse brain homogenates on western blots. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Parvalbumin alpha
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human,reacts with Human, Rat, Mouse. Antibody is specific for parvalbumin and does not recognize closely related proteins calretinin and calbindin as determined by Western Blotting.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Mouse anti-Methyl-CpG binding protein 2 (MeCP2) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Full-length recombinant human protein
Applications:
IHC-Frozen,WB
Clone number:
4F11
Antibody Isotype:
IgG1
Application Details:
Western blotting (1:5,000-1:10,000) and Immunohistochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
MeCP2, MeCp-2 protein
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human,reacts with Human, Mouse, Rat.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Mouse anti-Ki-67 Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human Ki-67 protein (amino acids 1,111-1,490) expressed in and purified from <i>E. coli.</i>
Applications:
ICC,WB
Clone number:
6B4
Antibody Isotype:
IgG1
Application Details:
Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:2,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
KI-67
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human,reacts with Human only. Does not react with Mouse or Rat.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Full-length recombinant human protein
Applications:
ICC,WB
Clone number:
1H10
Antibody Isotype:
IgG1
Application Details:
Western blotting (1:5,000-1:20,000) and Immunocytochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human,reacts with Human, Rat, Mouse.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Mouse anti-Calretinin-binding protein (CR) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Calretinin is a calcium-binding protein which is abundant in auditory neurons. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Rat
Immunogen:
Full-length recombinant human protein
Applications:
IHC-Frozen,WB
Clone number:
3G9
Antibody Isotype:
IgG1
Application Details:
Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CR, 29 kDa calbindin
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human, reacts with Human, Cow, Rat, Mouse. Antibody is specific for calbindin and does not recognize closely related proteins parvalbumin, calretinin and secretagogin as determined by Western Blotting.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Mouse anti-Calbindin-binding protein Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Buffers cytosolic calcium. May stimulate a membrane Ca<sup>2+</sup>-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Chicken,Horse,Human,Mouse,Pig,Rat
Immunogen:
Full-length recombinant human protein
Applications:
IHC-Frozen,WB
Clone number:
4H7
Antibody Isotype:
IgG1
Application Details:
Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human, reacts with Human, horse, Cow, Pig, chicken, Rat, Mouse.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Mouse anti-S-arrestin (S-AG) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Binds to photoactivated, phosphorylated RHO and terminates RHO signaling via G-proteins by competing with G-proteins for the same binding site on RHO (PubMed: 8003967, PubMed: 25205354). May play a role in preventing light-dependent degeneration of retinal photoreceptor cells (By similarity). Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Pig
Immunogen:
Recombinant bovine arrestin-1 with the first 20 amino acids of the C-terminus truncated
Applications:
IHC-Frozen,WB
Clone number:
S128
Antibody Isotype:
IgG1
Application Details:
Western blotting (1:5,000) and Immunohistochemistry (1:1,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
48 kDa protein, Retinal S-antigen, S-AG, Rod photoreceptor arrestin
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Bovine, reacts with Pig. Other species not yet tested. Antibody detects arrestin-1 protein only and does not cross-react with the other 3 arrestin molecules.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
X63-saporin is an antibody conjugate comprising of the non-specific monoclonal IgG1 antibody X63, chemically conjugated via a reducible disulfide bridge to the ribosome-inactivating protein saporin, purified from saponaria officinalis . Antibody clone X63 has no known binding ability, and thus can be used as negative control antibody. In combination with saporin, X63-saporin is a useful negative control for targeting IgG 1 -saporin conjugates such as MC192-saporin.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a 1 mg/mL solution containing PBS pH 7.2-7.6 without preservative.
Host Animal:
Mouse
Species Reactivity:
Non-Reactive (Negative Control)
Immunogen:
Unknown, naturally isolated IgG that has no known binding target in mammals
Applications:
Negative Control
Clone number:
X63
Antibody Isotype:
IgG1, kappa
Application Details:
Negative control for targeting IgG1-saporin conjugates., for instance MC192-saporin. X63-saporin should be used at a concentration equal to that of the test antibody-saporin conjugate.
Biological Activity:
None. Routinely tested for absence of killing of rat C6 cells in vitro. Note that the primary use of X63-saporin is for in vivo applications.
Biosensis Brand:
Biosensis®
Conjugate:
Saporin
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
No staining has ever been identified with this immunoglobulin demonstrating its non-specific value as a control.
Storage:
Lyophilized product is shipped at ambient room temperature. Upon receipt, pulse-centrifuge the vial to collect solid that may be entrapped in the lid. After reconstitution, immediately prepare aliquots and keep the undiluted stock at -80°C for long-term storage. Avoid repeated thaw-freezing. For short-term storage, keep at 2-8°C for up to 2 weeks. it is recommended to handle this product under sterile conditions.
Purification:
Conjugate was purified by ion-exchange chromatography. Purity > 90% by non-reducing SDS-PAGE
X63-saporin is an antibody conjugate comprising of the non-specific monoclonal IgG 1 antibody X63, chemically conjugated via a reducible disulfide bridge to the ribosome-inactivating protein saporin, purified from saponaria officinalis . Antibody clone X63 has no known binding ability, and thus can be used as negative control antibody. In combination with saporin, X63-saporin is a useful negative control for targeting IgG 1 -saporin conjugates such as MC192-saporin.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a 1 mg/mL solution containing PBS pH 7.2-7.6 without preservative.
Host Animal:
Mouse
Species Reactivity:
Non-Reactive (Negative Control)
Immunogen:
Unknown, naturally isolated IgG that has no known binding target in mammals
Applications:
Negative Control
Clone number:
X63
Antibody Isotype:
IgG1, kappa
Application Details:
Negative control for targeting IgG1-saporin conjugates., for instance MC192-saporin. X63-saporin should be used at a concentration equal to that of the test antibody-saporin conjugate.
Biological Activity:
None. Routinely tested for absence of killing of rat C6 cells in vitro. Note that the primary use of X63-saporin is for in vivo applications.
Biosensis Brand:
Biosensis®
Conjugate:
Saporin
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
No staining has ever been identified with this immunoglobulin demonstrating its non-specific value as a control.
Storage:
Lyophilized product is shipped at ambient room temperature. Upon receipt, pulse-centrifuge the vial to collect solid that may be entrapped in the lid. After reconstitution, immediately prepare aliquots and keep the undiluted stock at -80°C for long-term storage. Avoid repeated thaw-freezing. For short-term storage, keep at 2-8°C for up to 2 weeks. it is recommended to handle this product under sterile conditions.
Purification:
Conjugate was purified by ion-exchange chromatography. Purity > 90% by non-reducing SDS-PAGE
X63-saporin is an antibody conjugate comprising of the non-specific monoclonal IgG 1 antibody X63, chemically conjugated via a reducible disulfide bridge to the ribosome-inactivating protein saporin, purified from saponaria officinalis . Antibody clone X63 has no known binding ability, and thus can be used as negative control antibody. In combination with saporin, X63-saporin is a useful negative control for targeting IgG1-saporin conjugates such as MC192-saporin.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a 1 mg/mL solution containing PBS pH 7.2-7.6 without preservative.
Host Animal:
Mouse
Species Reactivity:
Non-Reactive (Negative Control)
Immunogen:
Unknown, naturally isolated IgG that has no known binding target in mammals
Applications:
Negative Control
Clone number:
X63
Antibody Isotype:
IgG1, kappa
Application Details:
Negative control for targeting IgG1-saporin conjugates., for instance MC192-saporin. X63-saporin should be used at a concentration equal to that of the test antibody-saporin conjugate.
Biological Activity:
None. Routinely tested for absence of killing of rat C6 cells in vitro. Note that the primary use of X63-saporin is for in vivo applications.
Biosensis Brand:
Biosensis®
Conjugate:
Saporin
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
No staining has ever been identified with this immunoglobulin demonstrating its non-specific value as a control.
Storage:
Lyophilized product is shipped at ambient room temperature. Upon receipt, pulse-centrifuge the vial to collect solid that may be entrapped in the lid. After reconstitution, immediately prepare aliquots and keep the undiluted stock at -80°C for long-term storage. Avoid repeated thaw-freezing. For short-term storage, keep at 2-8°C for up to 2 weeks. it is recommended to handle this product under sterile conditions.
Purification:
Conjugate was purified by ion-exchange chromatography. Purity > 90% by non-reducing SDS-PAGE
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.<br /><br />Biosensis now offers <strong>biotinylated proNGF antibody</strong> allowing more flexibility in experimental design by using the biotin-avidin/streptavidin detection method. The ability of biotinylated proNGF antibody to detect proNGF has been validated by WB.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS buffer pH 7.4 with 3% trehalose, without preservatives.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
A synthetic peptide (C-HTIPQAHWTKLQ, aa: 30-41) of human proNGF protein has been used as the immunogen. The sequence is located on the pro-domain of the proNGF full-length protein and is 80% homologous to mouse and rat proNGF.
Applications:
WB
Clone number:
BS312
Antibody Isotype:
IgG2, lambda
Application Details:
The biotinylated proNGF antibody has been tested by Western Blotting (0.1-0.5 µg/mL) and is also expected to work in applications validated for the unlabelled antibody M-1738-100 at same or higher dilutions: Flow Cytometry and Immunofluorescence.<br><br>Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Pro-brain nerve growth factor; proNGF; NGF
Biosensis Brand:
Biosensis®
Conjugate:
Biotin
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human Species cross-reactivity not tested.
Storage:
After reconstitution divide into aliquots and store at -20°C for a higher stability. Antibody contains no preservatives. Store at 2-8°C with an appropriate antibacterial agent. Use sterile methods. Highest purity Glycerol (1:1) may be added for an additional stability when stored at refrigerated or freezing temperatures. Avoid repetitive freeze/thaw cycles.
Purification:
Antibody was purified from cell culture supernatant by Protein G chromatography, biotinylated and buffer-exchanged into PBS, pH 7.4 buffer
Stapledon CJM et al. (2019). Human osteocyte expression of Nerve Growth Factor: The effect of Pentosan Polysulphate Sodium (PPS) and implications for pain associated with knee osteoarthritis. PLoS One. 14(9):e0222602. Application: ICC/IF.
Specificity:
No staining has ever been identified with this immunoglobulin demonstrating its value as a control. N/A
Storage:
The antibody conjugate can be stored at 2-8ºC for up to 4 months with the addition of appropriate antibacterial agent. For long-term storage, aliquot and keep antibody at <-20°C in the dark. Avoid repeated freeze-thaw cycles.
Purification:
FITC-labelled X63 antibody was purified by gel filtration.
MC192-saporin is an antibody conjugate comprising of the monoclonal antibody MC192 against rat p75 NTR , the nerve growth factor receptor, chemically conjugated via a reducible disulfide bridge to the ribosome-inactivating protein saporin, purified from saponaria officinalis . Unconjugated saporin is incapable of entering the cells due to the apparent lack of ligand. Upon specific binding via MC192 to the cells expressing p75 NTR , saporin transverses the cell membrane leading to lesion of neurochemically defined neuronal populations. The targets of MC192-Saporin are p75 NTR -expressing cells including cholinergic neurons of the basal forebrain, cerebellar Purkinje cells, medial septum, diagonal band of Broca, Nucleus basalis of Meynert and some tumour cells. MC192-saporin has been used in the study of learning and memory and its primary application is in vivo , MC192-saporin is specific for applications in rat. The antibody does not cross-react with human or mouse p75 NTR receptors.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a 1 mg/mL solution containing PBS pH 7.2-7.6 without preservative.
Host Animal:
Mouse
Species Reactivity:
Rat
Immunogen:
Rat NGF receptor (p75NTR)
Applications:
In-vivo
Clone number:
MC192
Antibody Isotype:
IgG1
Application Details:
1. To specifically target and eliminate rat cells expressing p75NTR <i>in vivo</i>. MC192-saporin has been used to selectively lesion cholinergic neurons of basal forebrain to create an animal model to study Alzheimer's disease. <br> 2. To be used as a model for gene delivery into neurons.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Active. Ablates p75-positive cells in rat in vivo. Routinely tested for dose-dependent killing of rat C6 cells in vitro. Note that the primary use of MC192-saporin is for in vivo applications in rat. Effective MC192-saporin concentrations must be determined for every new batch.
Biosensis Brand:
Biosensis®
Conjugate:
Saporin
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
MC192 antibody is specific only for rat NGFR, no reactivity to human or mouse NGFR has been reported This monoclonal antibody does not cross react with p75NTR-expressing cells in other species than rat.
Storage:
Lyophilized product is shipped at ambient room temperature. Upon receipt, pulse-centrifuge the vial to collect solid that may be entrapped in the lid. After reconstitution, immediately prepare aliquots and keep the undiluted stock at -80°C for long-term storage. Avoid repeated thaw-freezing. For short-term storage, keep at 2-8°C for up to 2 weeks. it is recommended to handle this product under sterile conditions.
Purification:
Conjugate was purified by ion-exchange chromatography. Purity > 90% by non-reducing SDS-PAGE
MC192-saporin is an antibody conjugate comprising of the monoclonal antibody MC192 against rat p75 NTR , the nerve growth factor receptor, chemically conjugated via a reducible disulfide bridge to the ribosome-inactivating protein saporin, purified from saponaria officinalis . Unconjugated saporin is incapable of entering the cells due to the apparent lack of ligand. Upon specific binding via MC192 to the cells expressing p75 NTR , saporin transverses the cell membrane leading to lesion of neurochemically defined neuronal populations. The targets of MC192-Saporin are p75 NTR -expressing cells including cholinergic neurons of the basal forebrain, cerebellar Purkinje cells, medial septum, diagonal band of Broca, Nucleus basalis of Meynert and some tumour cells. MC192-saporin has been used in the study of learning and memory and its primary application is in vivo , MC192-saporin is specific for applications in rat. The antibody does not cross-react with human or mouse p75 NTR receptors.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a 1 mg/mL solution containing PBS pH 7.2-7.6 without preservative.
Host Animal:
Mouse
Species Reactivity:
Rat
Immunogen:
Rat NGF receptor (p75NTR)
Applications:
In-vivo
Clone number:
MC192
Antibody Isotype:
IgG1
Application Details:
1. To specifically target and eliminate rat cells expressing p75NTR <i>in vivo</i>. MC192-saporin has been used to selectively lesion cholinergic neurons of basal forebrain to create an animal model to study Alzheimer's disease. <br> 2. To be used as a model for gene delivery into neurons.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Active. Ablates p75-positive cells in rat in vivo. Routinely tested for dose-dependent killing of rat C6 cells in vitro. Note that the primary use of MC192-saporin is for in vivo applications in rat. Effective MC192-saporin concentrations must be determined for every new batch.
Biosensis Brand:
Biosensis®
Conjugate:
Saporin
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
MC192 antibody is specific only for rat NGFR, no reactivity to human or mouse NGFR has been reported This monoclonal antibody does not cross react with p75NTR-expressing cells in other species than rat.
Storage:
Lyophilized product is shipped at ambient room temperature. Upon receipt, pulse-centrifuge the vial to collect solid that may be entrapped in the lid. After reconstitution, immediately prepare aliquots and keep the undiluted stock at -80°C for long-term storage. Avoid repeated thaw-freezing. For short-term storage, keep at 2-8°C for up to 2 weeks. it is recommended to handle this product under sterile conditions.
Purification:
Conjugate was purified by ion-exchange chromatography. Purity > 90% by non-reducing SDS-PAGE
MC192-saporin is an antibody conjugate comprising of the monoclonal antibody MC192 against rat p75 NTR , the nerve growth factor receptor, chemically conjugated via a reducible disulfide bridge to the ribosome-inactivating protein saporin, purified from saponaria officinalis . Unconjugated saporin is incapable of entering the cells due to the apparent lack of ligand. Upon specific binding via MC192 to the cells expressing p75 NTR , saporin transverses the cell membrane leading to lesion of neurochemically defined neuronal populations. The targets of MC192-Saporin are p75 NTR -expressing cells including cholinergic neurons of the basal forebrain, cerebellar Purkinje cells, medial septum, diagonal band of Broca, Nucleus basalis of Meynert and some tumour cells. MC192-saporin has been used in the study of learning and memory and its primary application is in vivo , MC192-saporin is specific for applications in rat. The antibody does not cross-react with human or mouse p75 NTR receptors.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a 1 mg/mL solution containing PBS pH 7.2-7.6 without preservative.
Host Animal:
Mouse
Species Reactivity:
Rat
Immunogen:
Rat NGF receptor (p75NTR)
Applications:
In-vivo
Clone number:
MC192
Antibody Isotype:
IgG1
Application Details:
1. To specifically target and eliminate rat cells expressing p75NTR <i>in vivo</i>. MC192-saporin has been used to selectively lesion cholinergic neurons of basal forebrain to create an animal model to study Alzheimer's disease. <br> 2. To be used as a model for gene delivery into neurons.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Active. Ablates p75-positive cells in rat in vivo. Routinely tested for dose-dependent killing of rat C6 cells in vitro. Note that the primary use of MC192-saporin is for in vivo applications in rat. Effective MC192-saporin concentrations must be determined for every new batch.
Biosensis Brand:
Biosensis®
Conjugate:
Saporin
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
MC192 antibody is specific only for rat NGFR, no reactivity to human or mouse NGFR has been reported This monoclonal antibody does not cross react with p75NTR-expressing cells in other species than rat.
Storage:
Lyophilized product is shipped at ambient room temperature. Upon receipt, pulse-centrifuge the vial to collect solid that may be entrapped in the lid. After reconstitution, immediately prepare aliquots and keep the undiluted stock at -80°C for long-term storage. Avoid repeated thaw-freezing. For short-term storage, keep at 2-8°C for up to 2 weeks. it is recommended to handle this product under sterile conditions.
Purification:
Conjugate was purified by ion-exchange chromatography. Purity > 90% by non-reducing SDS-PAGE
FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily. SIMILARITY: Contains 1 bZIP domain (Ref: uniprot.org).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Chicken,Horse,Human,Mouse,Pig,Rat
Immunogen:
Full length, E.coli-derived recombinant human c-FOS protein.
Applications:
ICC,IHC-Frozen
Clone number:
2H2
Antibody Isotype:
IgG1
Application Details:
Immunohistochemistry (IHC) and immunocytochemistry (ICC): 1-2 µg/mL. This antibody has been shown to work on 4% PFA fixed mouse brain sections.<br><br>Western blotting (WB): 0.5-1.0 µg/mL. This antibody detects bands between 50-65 kDa, which only appear in stimulated cells.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Cellular oncogene fos; G0/G1 switch regulatory protein 7; cFOS
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Choi S et al. (2020). Parallel ascending spinal pathways for affective touch and pain. Nature. 587(7833):258-263. Application: IHC . Species: Mouse . Bai L et al. (2019). Genetic Identification of Vagal Sensory Neurons That Control Feeding. Cell. 179(5):1129-43. Application: IHC . Species: Mouse .
Specificity:
Human. Horse, cow, pig, chicken, rat, mouse.
Storage:
Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 0.1% trehalose, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human mature BDNF expressed in E.coli
Applications:
ELISA,WB
Clone number:
4C8
Antibody Isotype:
IgG
Application Details:
<b>ELISA:</b> 50-100 ng/mL <br><br><b>Western Blotting (denaturing and reducing):</b> 0.2 to 2 µg/mL. M-1744-100 antibody detects 14 kDa mature BDNF monomer in human serum human and acid treated human and rodent whole tissue homogenates. In simple Tris-homogenate human brain lysates, M-1744-100 detects mature BDNF at 14kDa as well as an uncharacterized band at ~50kDa. No proBDNF band is detected in our samples, although M-1744-100 will detect proBDNF in purified form on blots. Additionally, M-1744-100 detects an additional band 18 kDa BDNF isoform human serum. For cell lysates, some caution is advised as in our hands M-1744-100 fails to detect mature BDNF on SH-SY5Y or C6 cell lysates but does detect mature BDNF in neuronal cell culture lysates. The reason for these differences has not been characterized. For detection of mature BDNF in cell lysates such as SH-SY5Y we recommend affinity-purified rabbit polyclonal antibody to rhBDNF (R-1707-100), or rabbit polyclonal antibody to BDNF peptide 1-10 (R-083-100, whole serum; R-066-500, IgG).<br><br><b>Immunofluorescence:</b> 1 to 4 µg/mL, 4% formaldehyde fixation and permeabilization. Staining can be weak until optimized.<br><br>M-1744-100 is not recommended for Flow Cytometry at this time.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Le Blanc J et al. (2020) Platelets Selectively Regulate the Release of BDNF, But Not That of Its Precursor Protein, proBDNF. Front Immunol. 11:575607 Application: Human, WB.
Specificity:
Detects human, mouse, rat, guinea pig BDNF. Expected to detect BDNF from other species due to sequence homology.
Storage:
Store lyophilized antibody at -20°C to -80°C protected from moisture. After reconstitution divide antibody into useful aliquots and keep aliquots at -20°C to -80°C for a higher stability. Working aliquots can be kept at 2-8°C for up to 1 month. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 0.1% trehalose, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human mature BDNF expressed in E.coli
Applications:
ELISA,WB
Clone number:
4C8
Antibody Isotype:
IgG
Application Details:
<b>ELISA:</b> 50-100 ng/mL <br><br><b>Western Blotting (denaturing and reducing):</b> 0.2 to 2 µg/mL. M-1744-100 antibody detects 14 kDa mature BDNF monomer in human serum human and acid treated human and rodent whole tissue homogenates. In simple Tris-homogenate human brain lysates, M-1744-100 detects mature BDNF at 14kDa as well as an uncharacterized band at ~50kDa. No proBDNF band is detected in our samples, although M-1744-100 will detect proBDNF in purified form on blots. Additionally, M-1744-100 detects an additional band 18 kDa BDNF isoform human serum. For cell lysates, some caution is advised as in our hands M-1744-100 fails to detect mature BDNF on SH-SY5Y or C6 cell lysates but does detect mature BDNF in neuronal cell culture lysates. The reason for these differences has not been characterized. For detection of mature BDNF in cell lysates such as SH-SY5Y we recommend affinity-purified rabbit polyclonal antibody to rhBDNF (R-1707-100), or rabbit polyclonal antibody to BDNF peptide 1-10 (R-083-100, whole serum; R-066-500, IgG).<br><br><b>Immunofluorescence:</b> 1 to 4 µg/mL, 4% formaldehyde fixation and permeabilization. Staining can be weak until optimized.<br><br>M-1744-100 is not recommended for Flow Cytometry at this time.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Le Blanc J et al. (2020) Platelets Selectively Regulate the Release of BDNF, But Not That of Its Precursor Protein, proBDNF. Front Immunol. 11:575607 Application: Human, WB.
Specificity:
Detects human, mouse, rat, guinea pig BDNF. Expected to detect BDNF from other species due to sequence homology.
Storage:
Store lyophilized antibody at -20°C to -80°C protected from moisture. After reconstitution divide antibody into useful aliquots and keep aliquots at -20°C to -80°C for a higher stability. Working aliquots can be kept at 2-8°C for up to 1 month. Avoid repetitive freeze/thaw cycles.
The amyloid beta peptide is derived from the cleavage of the Amyloid precursor protein (APP) and varies in length from 39 to 43 amino acids. However, the form(s) of amyloid-beta peptide (A? associated with the pathology characteristic of Alzheimer's disease (AD) remains unclear. In particular, the neurotoxicity of intraneuronal A? accumulation is an area of considerable research and controversy principally because antibodies thought to be specific for A? have been shown to actually detect intraneuronal APP and not A? exclusively.<br /><br />MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to A? residues 1-4 as demonstrated by biochemical and immunohistochemical analyses (IHC), and is highly specific just to amyloid beta peptide. <strong>MOAB-2 did not detect APP or APP-CTFs</strong> in cell culture media/lysates (HEK-APPSwe or HEK APPSwe/BACE1) or in brain homogenates from transgenic mice expressing 5 familial AD (FAD) mutation (5xFAD mice). <br /><br />Using IHC on 5xFAD brain tissue, MOAB-2 immunoreactivity co-localized with C-terminal antibodies specific for A?40 and A?42. MOAB-2 did not co-localize with either N- or C-terminal antibodies to APP. In addition, no MOAB-2-immunreactivity was observed in the brains of 5xFAD/BACE-/- mice, although significant amounts of APP were detected by N- and C-terminal antibodies to APP, as well as by 6E10. In both 5xFAD and 3xTg mouse brain tissue, MOAB-2 co-localized with cathepsin-D, a marker for acidic organelles, further evidence for intraneuronal A?, distinct from A? associated with the cell membrane. MOAB-2 demonstrated strong intraneuronal and extra-cellular immunoreactivity in 5xFAD and 3xTg mouse brain tissues.<br /><br />Biosensis now offers <strong>biotinylated MOAB-2</strong> <strong>antibody</strong> allowing more flexibility in experimental design by using the biotin-avidin/streptavidin detection method. Biotinylated MOAB-2 antibody may also help to reduce background staining in difficult-to-stain tissues and increase detection sensitivity. The ability of biotinylated MOAB-2 antibody to detect amyloid beta has been validated by IHC.<br /><br />Purified, non-biotinylated MOAB-2 antibody is available <a href="https://www.biosensis.com/moab-mouse-monoclonal-antibody-amyloid-beta-peptide-beta-4042-purified-p-1181.htmL">here</a>.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer, pH 7.4; contains no preservative.
Host Animal:
Mouse
Species Reactivity:
Human,Rat
Immunogen:
Recombinant human amyloid beta protein 42 (A?42): DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
Applications:
ELISA,ICC,IHC-Frozen,IHC-Paraffin-embedded,IP,WB
Clone number:
MOAB-2
Antibody Isotype:
IgG2b, lambda
Application Details:
The biotinylated MOAB-2 antibody has been tested by IHC (1:500 - 1:2,000 dilution) and is also expected to work in applications validated for the unlabelled antibody (M-1586-100) at same or higher dilutions: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry/paraffin embedded IHC(P), Immunoprecipitation (IP), Immunofluorescence (IF), ELISA.<br><br><i>Western Blotting:</i><br><br>MOAB-2 has been tested in WB using purified synthetic beta-amyloid preparations and from transgenic mouse brain formic acid extracts (see Figure 1). Formic acid extraction/concentration is required for western blot detection from extracts. Suggested dilution of 1:2000-1:5,000 for WB, standard ECL detection systems. <br><br>Tissue samples for the detection of beta-amyloid should be prepared as detailed in Youmans KL et al., 2011 (Journal of Neuroscience Methods 196: 51-59) for best results. Detection of beta-amyloid 40/42 in direct westerns can be difficult; Dot-blots of prepared samples are recommended as detailed in Youmans KL et al., 2012. <br><br><i>Immunohistochemistry:</i><br><br>Suggested dilution for biotinylated MOAB-2 in IHC is 1:500-1:2,000. Fresh frozen, 4% paraformaldehyde fixed frozen, or formalin fixed paraffin embedded tissues are all suitable. Antigen retrieval is required in fixed tissues for optimal staining.<br><br>Antibody was tested on 4% paraformaldehyde/0.1% glutaraldehyde fixed frozen tissue from 3xTg and 5xFAD mice. MOAB-2 antibody detects intraneuronal and extracellular beta-amyloid in IHC and does not detect APP (Youmans KL et al., 2012).<br><br>The antibody also reacts with archival formalin-fixed, paraffin-embedded tissue samples with antigen Heat Induced Epitope Retrieval (HIER). Recommended buffer for HIER is citrate, pH 6.0. Signal was weak without antigen retrieval. Immunoreactivity was observed in intraneural-amyloid deposition (plaque) in Alzheimer's brain. MOAB-2 was found to be extremely clean and with an excellent signal to noise ratio with no neuro-cellular diffusive staining.<br><br>In addition, MOAB-2 demonstrated no significant differences in A-beta detection using paraffin fixed, free-floating sections (Youmans KL et al., 2012). Formic acid (FA) treatment resulted in optimal detection of both intraneuronal and extracellular A-beta compared to without FA (incubated in 88% FA 8 min, Youmans KL et al., 2012). Free floating tissue sections were permeabilized in TBS containing 0.25% Triton X-100 (TBSX; 3 x 10 min), blocked with 3% horse serum in TBSX (3 x 10 min) followed by 1% horse serum in TBSX (2 x10 min) and incubated with appropriate primary antibodies diluted in TBSX containing 1% horse serum overnight. See Youmans KL et al., 2012, for full IHC(P) protocol and method details.<br><br><i>Immunofluorescence:</i><br><br>For IF, suggested dilution is 1:100-1:500. The antibody was tested on 4% PFA fixed frozen tissue. Fixed tissues were washed in TBS (3 x 10 min), then incubated in 88% FA (8 min), and then permeabilized in TBSX (3 x 10 min), and blocked in TBSX containing 5% bovine serum albumin (BSA; 1 hr). Sections were subsequently incubated with appropriate primary antibodies diluted in TBSX containing 2% BSA overnight on an oscillatory rotator. Detection was via fluorescently labelled absorbed secondary antibodies (Youmans KL et al., 2012).<br><br><i>Immunoprecipitation:</i><br><br>For IP, the suggested dilution is 1:200 to 1:1,000 for labelled beta-amyloid using SA-coated beads as the capture vehicle, similar to the protocols employed by Youmans KL et al., 2012.<br><br><i>ELISA:</i><br><br>In an ELISA, a dilution of 1:50-1:1,000 is suggested. The antibody has been tested in ELISAs on synthetic beta-amyloid and tissue homogenates from beta-amyloid-Tg mice. <br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user for all applications. Dilutions provided are only meant to serve as a basic guide.
Kim, S. et al. (2020) Performance Validation of a Planar Hall Resistance Biosensor through Beta-Amyloid Biomarker. Sensors (Basel). 20(2) Application: In-vitro biosensor. Ruan, CS. et al. (2017) Sortilin inhibits amyloid pathology by regulating non-specific degradation of APP. Exp Neurol. [Epub ahead of print] Application: IHC References for non-biotinylated MOAB-2 antibody (M-1586-100): Zhu, B. et al. (2017) ER-associated degradation regulates Alzheimer's amyloid pathology and memory function by modulating _-secretase activity. Nat Commun. 8(1):1472. Application: IHC Huang, TY. et al. (2017) SORLA attenuates EphA4 signaling and amyloid _-induced neurodegeneration. J Exp Med. pii: jem.20171413. [Epub ahead of print]. Application: IHC Felecia, M. et al. (2017) Peripheral Inflammation, Apolipoprotein E4, and Amyloid-_ Interact to Induce Cognitive and Cerebrovascular Dysfunction. ASN Neuro. 9(4):1759091417719201. Application: IHC/IF Thomas, R. et al. (2016) Epidermal growth factor prevents APOE4 and amyloid-beta-induced cognitive and cerebrovascular deficits in female mice. Acta Neuropathol Commun. 4(1):111 Application: IHC Koster, KP. et al. (2016) Epidermal growth factor prevents oligomeric amyloid-_ induced angiogenesis deficits in vitro. J Cereb Blood Flow Metab. [Epub ahead of print] Application: IF Loffler, T. et al. (2016) Decreased Plasma A? in Hyperlipidemic APPSL Transgenic Mice Is Associated with BBB Dysfunction. Front. Neurosci. Application: IF Kobro-Flatmoen, A. et al. (2016) Reelin-immunoreactive neurons in entorhinal cortex layer II selectively express intracellular amyloid in early Alzheimer's disease. Neurobiology of Disease. 93:172-183. Application: IHC Tai, LM. et al. (2016) The role of APOE in cerebrovascular dysfunction. Acta Neuropathol. 131(5):709-23. Application: IF Kim, YH. et al. (2015) A 3D human neural cell culture system for modeling Alzheimer's disease. Nat Prot. 10(7):985-1006. Application: WB Condello, C. et al. (2015) Microglia constitute a barrier that prevents neurotoxic protofibrillar A?42 hotspots around plaques. Nat Commun. 6:6176. Application: IF Iulita MF et al (2014) Intracellular Abeta pathology and early cognitive impairments in a transgenic rat model overexpressing human amyloid precursor protein: a multidimensional study. Acta Neuropathol Commun. 6:61. Application: IF, IH Smith BR et al (2014) Neuronal inclusions of alpha-synuclein contribute to the pathogenesis of Krabbe disease. J Pathol. Apr;235(5):509-21. Application: IF
Specificity:
MOAB-2 detects preparations enriched in U-, O-, F-A?42, and U-A?40 by dot-blot, and is thus a pan-specific A? antibody. However, MOAB-2 is selective for the more neurotoxic A?42 compared to A?40. Indeed, MOAB-2 demonstrated a titration against antigen concentration, and detects A?40 at 2.5 pmol, but U-, O- and F-A?b42 at antigen concentrations as low as ~ 0.1 pmol (Youmans. KL et al., 2012; PMID: 22423893). MOAB-2 does not detect APP (Amyloid Precursor Protein). Human, rat, other species not yet tested. By Dot Blot, MOAB-2 detected rat A?40 and human A?40, albeit with less affinity than for A?42 (Youmans KL et al., 2012).
Storage:
After reconstitution keep aliquots at -20°C to -70°C for a higher stability. At 2-8°C keep up to one week; use sterile methods and pipettes. Highly purified glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. Keep tightly closed when not in use and protected from light.
Purification:
Antibody was purified from cell culture supernatant by Protein G chromatography, biotinylated and buffer-exchanged into PBS, pH 7.4 buffer
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS buffer pH 7.4 with 3% trehalose, without preservatives.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
A synthetic peptide (C-HTIPQAHWTKLQ, aa: 30-41) of human proNGF protein has been used as the immunogen. The sequence is located on the pro-domain of the proNGF full-length protein and is 80% homologous to mouse and rat proNGF.
Applications:
FC,ICC,WB
Clone number:
BS312
Antibody Isotype:
IgG2b, lambda
Application Details:
Flow Cytometry (2 ug/ 10^6 cells). Immunocytochemistry (1-2 µg/mL), Western Blotting (1-2 µg/mL). Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Pro-brain nerve growth factor; proNGF; NGF
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human Species cross-reactivity not tested.
Storage:
Store lyophilized antibody at 2-8ºC. After reconstitution divide into aliquots and store at -20°C for a higher stability. Antibody contains no preservatives. Store at 2-8°C with an appropriate antibacterial agent. Use sterile methods. Highest purity Glycerol (1:1) may be added for an additional stability when stored at refrigerated or freezing temperatures. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human mature BDNF expressed in E.coli
Applications:
ELISA,ICC,WB
Clone number:
3C11
Antibody Isotype:
IgG
Application Details:
ELISA (50-100 ng/mL), WB (0.2 to 2 µg/mL), IF (2 to 20 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Detects human, mouse, rat, guinea pig BDNF. Expected to detect BDNF from other species due to sequence homology.
Storage:
Store lyophilized antibody at -20°C to -80°C protected from moisture. After reconstitution divide antibody into useful aliquots and keep aliquots at -20°C to -80°C for a higher stability. Working aliquots can be kept at 2-8°C for up to 1 month. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human mature BDNF expressed in E.coli
Applications:
ELISA,ICC,WB
Clone number:
3C11
Antibody Isotype:
IgG
Application Details:
ELISA (50-100 ng/mL), WB (0.2 to 2 µg/mL), IF (2 to 20 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Detects human, mouse, rat, guinea pig BDNF. Expected to detect BDNF from other species due to sequence homology.
Storage:
Store lyophilized antibody at -20°C to -80°C protected from moisture. After reconstitution divide antibody into useful aliquots and keep aliquots at -20°C to -80°C for a higher stability. Working aliquots can be kept at 2-8°C for up to 1 month. Avoid repetitive freeze/thaw cycles.
Brain derived neurotrophic factor (BDNF) is synthesized as a precursor (proBDNF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proBDNF is synthesized in neurons and glia (eg., microglia), transported anterogradely and retrogradely and may be released in an activity dependent manner.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS buffer pH 7.4 with 3% trehalose, without preservatives.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse (Predicted),Rat
Immunogen:
A synthetic peptide (C-NGPKAGSRGLTS, aa: 47-58) of human proBDNF protein has been used as the immunogen. The sequence is located on the pro-domain of the proBDNF full-length protein.
Applications:
FC,ICC
Clone number:
BS375
Antibody Isotype:
IgG1, kappa
Application Details:
Flow Cytometry (2 ug/10<sup>6</sup> cells).<br>Immunocytochemistry (1-2 µg/mL).<br>Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Abrineurin
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human Not yet tested. Antibody may detect mouse and rat proBDNF protein due to high degree of amino acid sequence homology.
Storage:
After reconstitution divide into aliquots and store at -20°C for a higher stability. Antibody contains no preservatives. Store at 2-8°C with an appropriate antibacterial agent. Use sterile methods. Highest purity Glycerol (1:1) may be added for an additional stability when stored at refrigerated or freezing temperatures. Avoid repetitive freeze/thaw cycles.
Mouse anti-Pro-glial cell line-derived neurotrophic factor (proGDNF) Monoclonal Antibody (Unconjugated), suitable for ICC, FC.
Background Info:
Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake (Ref: uniprot.org). ProGDNF is the unprocessed precursor molecule of mature GDNF and exists as homodimer.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS buffer pH 7.4 with 3% trehalose, without preservatives.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse (Predicted),Rat
Immunogen:
A synthetic peptide (C-EDYPDQFDDVMD, aa: 55-66) of human proGDNF protein has been used as the immunogen. The sequence is located on the pro-domain of the proGDNF full-length protein and is homologous with mouse and rat form of proGDNF.
Applications:
FC,ICC
Clone number:
BS376
Antibody Isotype:
IgG3, kappa
Application Details:
Flow Cytometry (2 ug/ 10<sup>6</sup> cells). Immunocytochemistry (1-2 µg/mL). Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human Rat. Antibody is expected to detect mouse proGDNF protein due to 100% amino acid sequence homology.
Storage:
After reconstitution divide into aliquots and store at -20°C for a higher stability. Antibody contains no preservatives. Store at 2-8°C with an appropriate antibacterial agent. Use sterile methods. Highest purity Glycerol (1:1) may be added for an additional stability when stored at refrigerated or freezing temperatures. Avoid repetitive freeze/thaw cycles.
MAP1A and MAP1B are microtubule-associated protein which mediate the physical interactions between microtubules and components of the cytoskeleton (probably involved in autophagosome formation). MAP1A and MAP1B each consist of a heavy chain subunit and 3 different light chain subunits (LC1, LC2 and LC3). MAP1LC3A is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor form of MAP1LC3A is cleaved by APG4/ATG4B to form the cytosolic form LC3-1. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. MAP1LC3A is most abundant in heart, brain, liver, skeletal muscle and testis but is absent in thymus and peripheral leukocytes.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS, pH 7.4, containing 3% trehalose without preservatives.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse (Predicted),Rat
Immunogen:
A synthetic peptide (C-RSFADRCKEVQQI) corresponding to amino acids 11-23 of human MAP1LC3 A protein was conjugated to a carrier protein and the complex used as the immunogen. This human sequence is homologous with mouse and rat MAP1LC3 A.
Applications:
FC,ICC,WB
Clone number:
BS405
Antibody Isotype:
IgG2a, kappa
Application Details:
Flow Cytometry (20 µg/mL), Western blot (10 µg/mL), Immunocytochemistry (1-2 µg/mL). Other applications have not yet been tested. Biosensis recommends optimal dilutions and concentrations should be determined by the end user.
WB confirmed binding of the antibody to a broad protein band with a Molecular Weight of ~14 kDa. Rat. The antibody is expected to react with mouse MAP1LC3A protein due to 100% sequence homology.
Storage:
After reconstitution divide into aliquots and store at -20°C for a higher stability. Antibody contains no preservatives. Store at 2-8°C with an appropriate antibacterial agent. Use sterile methods. Highest purity Glycerol (1:1) may be added for an additional stability when stored at refrigerated or freezing temperatures. Avoid repetitive freeze/thaw cycles.
Mouse anti-Tyrosine Kinase Receptor A (TrkA) Monoclonal Antibody (Unconjugated), suitable for ICC, FC.
Background Info:
TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Cell membrane; single-pass type I membrane protein. Endocytosed to the endosomes upon treatment of cells with NGF. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. Both isoforms have similar biological properties. TISSUE SPECIFICITY: Isoform TrkA-II is primarily expressed in neuronal cells. Isoform TrkA-I is found in non-neuronal tissues. Mutations in TrkA have been associated with congenital insensitivity to pain, anhidrosis, self-mutalating behaviour, mental retardation and cancer (Reference: www.uniprot.com).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS, pH 7.4, containing 3% trehalose without preservatives.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
A synthetic peptide from the extracellular domain of human TrkA (C-SATVMKSGGLPS, aa: 235-246) has been used as the immunogen.
Applications:
FC,ICC
Clone number:
BS470
Antibody Isotype:
IgG3, kappa
Application Details:
Flow cytometry: 20 µg/mL. <br><br>Immunocytochemistry: 1-5 µg/mL.<br><br> Flow cytometry data and immunofluorescence staining of TrkA (extracellular domain) receptor in SHSY-5Y cells has shown that detection of TrkA expression depends on its cellular localization (membrane vs. internal stores). Thus, permeabilization of cells may be required, despite that clone BS470 was raised against the extracellular domain of TrkA.<br><br> Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TrkA. Does not cross-react with TrkB or TrkC. Reacts with human TrkA. While not tested yet, M-1723-100 is not expected to react with TrkA from other species due to amino acid sequence dissimilarity.
Storage:
After reconstitution divide into aliquots and store at -20°C for a higher stability. Antibody contains no preservatives. Store at 2-8°C with an appropriate antibacterial agent. Use sterile methods. Highest purity Glycerol (1:1) may be added for an additional stability when stored at refrigerated or freezing temperatures. Avoid repetitive freeze/thaw cycles.
Mouse anti-Tyrosine Kinase Receptor A (TrkA) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Cell membrane; single-pass type I membrane protein. Endocytosed to the endosomes upon treatment of cells with NGF. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. Both isoforms have similar biological properties. TISSUE SPECIFICITY: Isoform TrkA-II is primarily expressed in neuronal cells. Isoform TrkA-I is found in non-neuronal tissues. Mutations in TrkA have been associated with congenital insensitivity to pain, anhidrosis, self-mutalating behaviour, mental retardation and cancer (Reference: www.uniprot.com).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS, pH 7.4, containing 3% trehalose without preservatives.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide from the intracellular cytoplasmic domain of human TrkA (C-HGPDAKLLAGGE, aa: 604-615) has been used as the immunogen.
Applications:
ICC,WB
Clone number:
BS292
Antibody Isotype:
IgG3, kappa
Application Details:
WB: Western blotting: 1-3 µg/mL, antibody is suitable for reduced (with DTT or beta-mercaptoethanol) and non-reduced samples. Denatured but not reduced samples provides cleaner blot signals as demonstrated in our photographs. <br><br>Immunocytochemistry: 1-2 µg/mL. Antibody works on 4% formaldehyde-fixed cells. Note that cells require a permeabilization step, because the antibody detects a cytoplasmic epitope of TrkA.<br><br>Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TrkA. Does not cross-react with TrkB or TrkC. Reacts with human TrkA. Known to cross-react with TrkA from rat and mouse. Expected to cross-react with other mammalian species based on peptide antigen sequence similarity.
Storage:
After reconstitution divide into aliquots and store at -20°C for a higher stability. Antibody contains no preservatives. Store at 2-8°C with an appropriate antibacterial agent. Use sterile methods. Highest purity Glycerol (1:1) may be added for an additional stability when stored at refrigerated or freezing temperatures. Avoid repetitive freeze/thaw cycles.
Mouse anti-Capsaicin receptor (TrpV1) Monoclonal Antibody (Unconjugated), suitable for WB, FC.
Background Info:
The capsaicin receptor (VR1, TRPV1) is a ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. The receptor seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. It is involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca2+-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH less than 6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis (Ref: uniprot.org).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS, pH 7.4 with 3% trehalose.
Host Animal:
Mouse
Species Reactivity:
Guinea Pig (Predicted),Mouse,Rat
Immunogen:
A synthetic peptide (C-GSLKPEDAEVFKDSMVPGEK) as a part of the C-terminal rat VR1 protein (aa: 819-838) has been used as the immunogen.
Applications:
FC,WB
Clone number:
BS397
Antibody Isotype:
IgG2b, kappa
Application Details:
Flow Cytometry: 2 ug/10^6 cells. <br><br>Western blotting: 0.5-2 µg/mL , SDS-PAGE on Bis-Tris gel 4-12%, 5% beta-mercaptoethanol, primary antibody O/N incubation in 5% skim milk/TBST. Secondary is anti-mouse-HRP, 1/6000 dilution, 2h at room temperature. Blot developed on Li-Cor? C-DiGit? lot Scanner. <br><br>IHC: Frozen or PEG embedded tissues tested (PEG embedding, see Klosen P et al (1993) J Histochem Cytochem. 41(3):455-63). Conditions tested: 1-10 µg/mL in PBS, 48 hours, followed by detection via directly conjugated fluorescent anti-mouse secondary. Antibody not yet tested on paraffin embedded sections. Other immunohistochemistry methods not yet tested but are expected to be reactive. <br><br>ICC: 4% formaldehyde fixed cells tested; requires permeabilization step as antigen epitope is intracellular. Suggested primary antibody concentration: 1-2 µg/mL.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Matusica D et al. (2020) Differentiation of the 50B11 dorsal root ganglion cells into NGF and GDNF responsive nociceptor subtypes. Mol Pain. 16:1744806920970368 Application: Rat, WB. Bai J et al. (2018) [EXPRESS] Attenuation of TRPV1 by AMG-517 after Nerve Injury Promotes Peripheral Axonal Regeneration in Rats. Mol Pain. [Epub ahead of print] Application: Rat, WB.
Specificity:
Antibody is specific for rat/mouse VR1 protein in westerns and immunofluorescent immunohistochemistry on mouse PEG fixed DRG tissues. Pre-absorption with immunogen obliterates positive staining. Cross reactivity with other non-VR1 proteins is minimal; cross reactivity with VR1 from other species not yet tested. This antibody clone is known to react with rat and mouse TrpV1. It is predicted to react with guinea pig due to sequence homology.
Storage:
Store lyophilized antibody at 2-8ºC. After reconstitution divide in to aliquots and store at -20°C for a higher stability. Antibody contains no preservatives. Storage at 2-8°C with an appropriate antibacterial agent. USE Sterile methods. Highest purity Glycerol (1:1) may be added for an additional stability when stored at refrigerated or freezing temperatures. Avoid repetitive freeze/thaw cycles.
Mouse anti-Microtubule-associated protein tau (MAPT) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
FUNCTION: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. SUBCELLULAR LOCATION: Cytoplasm; cytosol. Cell membrane. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components. ALTERNATIVE PRODUCTS: 8 named isoforms produced by alternative splicing. Additional isoforms seem to exist. Isoforms differ from each other by the presence or absence of up to 5 of the 15 exons. One of these optional exons contains the additional tau/MAP repeat. TISSUE SPECIFICITY: Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system. DEVELOPMENTAL STAGE: Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain. DOMAIN: The tau/MAP repeat binds to tubulin. In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments and straight filaments, mainly composed of hyperphosphorylated forms of Microtubule-associated protein Tau. Defects in Microtubule-associated protein Tau are a cause of frontotemporal dementia and parkinsonism linked to chromosome 17, as well as a number of other neurodegenerative diseases.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant full length version of the shortest human tau isoform purified from E. coli.
Applications:
ICC,WB
Clone number:
2000000000
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; MAPT; MTBT1; TAU
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with multiple closely spaced bands covering the region of the blot from 48 kDa to 67 kDa. It has also been used successfully for immunocytochemistry.
Storage:
Maintain lyophilized material at 2-8°C. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-High-mobility group protein 1 (HMGP-1) Monoclonal Antibody (Unconjugated), suitable for WB, ICC, FC.
Background Info:
High-mobility group proteins were named originally since they are abundand relatively low molecular weight proteins which run quickly on SDS-PAGE gels. High-mobility group protein box 1 (HMGB1, Amphoterin) is one of these. The "bx" in the name refers to the so-called high mobility group (HMG) box, a compact domain involved in DNA binding and protein-protein interactions. the HMGB1 molecule has two of these HMG domains. The protein is alslo called amphoterin, this name being derived from the presence of two highly charged regions in the molecule, a relatively neutrally charged N-terminus and a very negatively charged C-terminus. In fact the molecule is very unusually charged throughout, the human sequence consisting of 16.7% Glutamic acid, 9.3% Aspartic acid, 20% lysine and 9.3% Arginine. HMGB1 can bind Toll like receptor 4 (TLR4) and the Receptor for Advanced Glycation End products (RAGE). TLRs are components of the innate immune system, first recognized as a family of receptors which recognize "Pathogen Associated Molecular Pattern molecules (PAMPs). PAMPs are common components of bacteria and when TLRs bind these a strong inflammatory response is activated. More recently it has been recognized that TLRs can also be activated by Damage Associated Molecular Pattern molecules (DAMPs), which are endogenous substances released from damaged and diseased cells which also bind to TLR family receptors and also activate inflammation. HMGB1 is such a DAMP, binding to TLR4, and much evidence suggests that HMGB1 is a strong activator of inflammation. Interestingly, HMGB1 is released by necrotic cells but not by apoptotic cells (1).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Human full length recombinant human HMGB1 protein expressed in and purified from E. coli.
Applications:
FC,ICC,WB
Clone number:
1F3
Antibody Isotype:
IgG2b
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Flow Cytometry. A dilution of 1:1,000 - 1:2,000 is recommended for WB. A dilution of 1:500 - 1:1,000 is recommended for ICC. For Flow Cytometry, use ~2 ug per 10^6 cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a band at ~25 kDa by Western blot on HeLa cell extract. It has also been used successfully for immunocytochemistry showing strong nuclear staining.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Aldolase C Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Aldolases are glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose 1,6-bisphosphate and fructose-1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde 3-phosphate or glyceraldehyde, respectively. Three aldolase isozymes are found in mammals specifically aldolases A, B, and C, each of which is encoded by a separate gene. Aldolase A is generally considered to be a muscle enzyme. Northern analysis of cultured cells suggests that it is present in both neurons and glia (1). Aldolase B is considered to be a liver-specific enzyme and it is transcriptionally activated by signals from hormones and dietary factors (2). In the adult, aldolase C is the brain-specific isozyme, with low but detectable activity in fetal tissues (1, 3-6). Aldolase C shares 81% amino acid identity with aldolase A and 70% identity with aldolase B. Earlier studies using isozyme-specific antibodies report its location in gray matter astrocytes and cells of the pia mater (5, 8). In situ hybridization of mouse central nervous system using isozyme-specific probes revealed that aldolase A and C are expressed in complementary cell types: aldolase A mRNA is found in neurons; aldolase C message is detected in astrocytes, some cells of the pia mater, and Purkinje cells (9). Aldolase C can in some situations be used as an astrocyte marker. However Purkinje cells of the cerebellum contain high levels of the enzyme, so the enzyme is not totally astrocyte specific.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
N-terminal 20 amino acids of aldolase C protein, MPHSYPALSAEQKKELSDIA
Applications:
ICC,WB
Clone number:
4A9
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:1,000 - 1:2,000 is recommended for WB. A dilution of 1:500 - 1:1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Brain-type aldolase, Fructose-bisphosphate aldolase C
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a 40 kDa band by Western blot on a crude bovine cerebellum homogenate. It has also been used successfully for immunocytochemistry.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Heat shock protein 27 (HSP-27) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
The heat shock proteins were discovered, as the name suggests, since they are heavily upregulated when cells are stressed by temperatures above the normal physiological range. They are expressed in unstressed cells also and have a normal function as chaperones, helping other proteins to fold correctly, and are required in much greater amounts if the cell or tissue is stressed by heat. The increased levels are generated transcriptionally under the influence of a powerful transcription factor, the heat shock factor 1 (HSF1). The different heat shock proteins were originally named based on their SDS-PAGE mobility, so HSP27 has an apparent molecular weight of 27 kDa. It is an abundant protein even under non-stress conditions and frequently shows up as a major spot on 2 dimensional gels of cells or tissues. It is known to associate with a variety of other proteins such as actin, intermediate filament subunits and ubiquitin and is found both in the cytoplasm and the nucleus of cells. HSP27 can become heavily phosphorylated under the influence of multiple protein kinases particularly as a result of activation of the p38/SAPK pathway. Upregulation of this protein is protective against neurodegenerative diseases at least in certain mouse models (1). Point mutations in the HSP27 gene are associated with two neurological diseases, Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuropathy IIB (2). These diseases are associated with axonal loss apparently following defects in the transport of neurofilaments.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant full length human HSP27 expressed in and purified from E. coli
Applications:
ICC,WB
Clone number:
6H11
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500 - 1:1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a 27 kDa band by Western blot on a crude extract from HeLa cells. It has also been used successfully for immunocytochemistry. Does not react with rodent protein.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Lysosomal Associated Membrane Protein 1 (LAMP1) Monoclonal Antibody (Unconjugated), suitable for WB, ICC, FC.
Background Info:
LAMP1 (Lysosomal Associated Membrane Protein 1, also known as CD107a, lysosomal associated membrane glycoprotein 1, LGP120 and LAMPA) is a protein primarily associated with the lysosomal membrane. In a typical cell LAMP1 is associated with spherical vesicles located next to the nucleus and the microtubule organizing center (1). LAMP1 is found on the cell surface of lymphocytes undergoing degranulation, a process in which cytoplasmic vesicles fuse with the plasma membrane, and this phenomena resulted in discovery of LAMP1 as a CD protein. The LAMP1 protein has a large N-terminal region which is inside the lysosome, hence topologically external to the cell, which is often referred to as the lumenal domain (2). The lumenal domain consists of two homologous globular segments separated by a proline rich sequence. Next there is a single membrane spanning domain and a short 11 amino acid C-terminal cytoplasmic tail. This tail region contains, at the extreme C-terminus, a so-called YXXI motif which is responsible for the sorting of the intact molecule to the endosome and lysozome, where Y = tyrosine, I = isoleucine and X = almost any amino acid (3). This motif is found in several other lysosomal proteins, where it functions in the same way. There are 417 amino acids in the human LAMP1 molecule, giving a native molecular weight of 44.8 kDa. However the N-terminal lumenal segment of LAMP1 is very heavily and variably glycosylated due to the presence of 18 N-linked glycosylation sites, so that on SDS-PAGE and on Western blots the protein runs as a diffuse band at 90-120 kDa. Antibodies to LAMP1 are therefore excellent markers of lysosomes in mammalian cells, though some LAMP1 may also be seen on late endosomes and on the plasma membrane.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant LAMP1 expressed and purified from E. coli.
Applications:
FC,ICC,WB
Clone number:
5H6
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry. A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:2,000 is recommended for IC. Use ~2ug per 10^6 cells for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Lysosomal Associated Membrane Protein 1, also known as CD107a, lysosomal associated membrane glycoprotein 1, LGP120 and LAMPA
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a diffuse band at ~90 kDa to 120 kDa by Western blot on HeLa cell extract. It has also been used successfully for immunocytochemistry showing strong punctate cytoplasmic staining corresponding to lysosomes and late endosomes. Does not react with rodent protein.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Lamin A/C Monoclonal Antibody (Unconjugated), suitable for WB, ICC, FC.
Background Info:
The Lamin proteins are members of the intermediate filament protein family but are located inside the nucleus rather than in the cytoplasm (1). The lamins function as skeletal components tightly associated with the inner nuclear membrane. Originally the proteins of the nuclear cytoskeleton were named Lamin A, B and C, from top to bottom as visualized on SDS-PAGE gels. Subsequently it was found that Lamins A and C were coded for by a single gene (2), while the Lamin B band may contain two proteins encoded by two genes now called Lamin B1 and Lamin B2. Lamin A has a mass of about 74 kDa while Lamin C is 65 kDa. The Lamin A protein includes 98 amino acids missing from Lamin C, while Lamin C has a C-terminal 6 amino acid peptide not present in Lamin A. Apart from these regions Lamin A and C are identical so that antibodies raised against either protein are likely to cross react with the other, as is the case with this monoclonal. Lamin polymerization and depolymerization is regulated by phosphorylation by cyclin dependent protein kinase 1 (CDK1), the key component of "maturation promoting factor", the central regulator of cell division. Activity of this kinase increases during cell division and is responsible for the breakdown of the nuclear lamina. Mutations in the LMNA gene are associated with several serious human diseases, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease type 2B1, and Hutchinson-Gilford progeria syndrome. This family of diseases belong to a larger group which are often referred to as Laminopathies, though some laminopathies are associated in defects in Lamin B1, B2 or one or other of the numerous nuclear lamina binding proteins. A truncated version of lamin A, commonly known as progerin, causes Hutchinson-Gilford progeria syndrome, a form of premature aging (3).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Full length recombinant human Lamin C
Applications:
FC,ICC,WB
Clone number:
4C4
Antibody Isotype:
IgG1
Application Details:
Immunocytochemistry (ICC), Western Blotting (WB) and Flow Cytometry (~2 ug per 10^6 cells). A dilution of 1:1,000-1:2,000 is recommended for WB. A dilution of 1:500-1:1,000 is recommended for ICC. The optimal dilution should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Does not react with mouse and rat.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Ataxin-2 binding protein 1 (A2BP1) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Fox1 is one of a family of 3 mammalian Fox1 homologues. Fox1 was discovered in C. elegans as a gene involved in sex determination, and the name Fox is an acronym of "Feminizing locus on X" (1). The C. elegans Fox1 protein and its 3 mammalian homologues are all about 46 kDa proteins and each includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70 amino acid structure consisting of 4 beta strands and two alpha-helices. This region is identical in all three mammalian Fox1 family proteins. An alternate name for Fox 1 is ataxin-2 binding protein 1 (A2BP1), since it was discovered in a yeast two hybrid screen using ataxin-2 as bait (2). As with the other Fox proteins, it is assumed that Fox1/A2BP1 has a role in the RNA splicing in the nervous system (3). Fox3, a protein also known as NeuN, is a widely used marker of neuronal nuclei and proximal cytoplasm (4, 5). Like Fox3, Fox1 is expressed in neuronal nuclei, but with a different pattern of expression than Fox3. For example, in the cerebellum Fox3 does not stain Purkinje neurons and Golgi neurons, but these two neuron types are stained by Fox1 antibody (6).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
N-terminal 100 amino acids of human Fox1 as expressed in and purified from E. coli.
Applications:
IHC-Frozen,WB
Clone number:
1G10
Antibody Isotype:
IgG1
Application Details:
Immunocytochemistry (IHC) and Western Blotting (WB). A dilution of 1:1,000-1:2,000 is recommended for WB. A dilution of 1:500-1:1000 is recommended for ICC. The optimal dilution should be determined by the end user.
Alternative Names:
ataxin-2 binding protein 1 (A2BP1); RNA binding protein fox-1 homolog 1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a 48 kDa and 46 kDa band by Western blot on extract from mouse brain. It has also been used successfully for immunocytochemistry on rat neuronal cultures.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
DNF (conserved dopamine neurotrophic factor) is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007; Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson's disease. When administered after 6-OHDA or MPTP -lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
PBS pH 7.4, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human CDNF protein produced using CHO-based cell line. Purified from cell culture supernatant.
Applications:
WB
Antibody Isotype:
IgG1
Application Details:
Western blot (WB) at a suggested dilution of 1:2,000 - 1:4,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
ARMETL1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
CDNF (conserved dopamine neurotrophic factor)
Storage:
Store at -20°C or -70°C upon receipt. After opening maintain undiluted in smaller aliquots at -20°C or -70°C for up to 6 months. Avoid multiple freeze-thaw cycles.
CDNF (conserved dopamine neurotrophic factor) is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- (Lindholm et al. 20007; Voutilainen et al., 2011) and MPTP-induced degeneration (Airavaara et al., 2012) of dopamine neurons in rodent models of Parkinson's disease. When administered after 6-OHDA or MPTP -lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
PBS pH 7.4, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human CDNF protein produced using CHO-based cell line. Purified from cell culture supernatant.
Applications:
WB
Antibody Isotype:
IgG1
Application Details:
Western blot (WB) at a suggested dilution of 1:2,000 - 1:4,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
ARMETL1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
CDNF (conserved dopamine neurotrophic factor)
Storage:
Store at -20°C or -70°C upon receipt. After opening maintain undiluted in smaller aliquots at -20°C or -70°C for up to 6 months. Avoid multiple freeze-thaw cycles.
MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson's disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
PBS pH 7.4, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human MANF protein produced using CHO-based cell line. Immunogen is purified from cell culture supernatant.
Applications:
WB
Antibody Isotype:
IgG1
Application Details:
Western blot (WB) at a suggested concentration of 0.5-2.0 µg/mL, Immunofluorescence 1-10 µg/mL and indirect ELISA 0.1-0.2 µg/mL. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Store at -20°C or -70°C upon receipt. After opening maintain undiluted in smaller aliquots at -20°C or -70°C for up to 6 months. Avoid multiple freeze-thaw cycles.
Mouse anti-Neurturin Monoclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Neurturin (NTN) is a member of the GDNF family of neurotrophic factors. This protein is a potent survival factor for several populations of central and peripheral neurons in mature and developing rodents. FUNCTION: Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. DISEASE: Defects in NRTN are a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, and possibly with other loci, defects in NRTN are involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
PBS pH 7.4, with 0.1% sodium azide
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human NRTN protein produced using CHO-based suspension cell line. Protein was purified from the cell culture supernatant.
Applications:
WB
Clone number:
1B11
Antibody Isotype:
IgG1
Application Details:
Western blot (WB) at a suggested dilution of 1:5,000-1:10,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human NTN (Neurturin)
Storage:
Store at -20°C or -70°C upon receipt. After opening divide antibody into smaller aliquots and store at -20°C or -70°C for up to six months. Avoid multiple freeze-thaw cycles as product degradation may result.
Mouse anti-Visinin-like protein 1 (VLP-1) Monoclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
Background Info:
Visinin (sometimes known as hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1) was originally isolated biochemically from chicken retina as a major protein of about 24 kDa on SDS-PAGE (1). Following cloning and sequencing of visinin, several visinin like proteins were discovered by homology screening (2, 3). One of these, Visinin-like protein 1 is a small Calcium binding protein which is very abundant in the nervous system and is found only in neurons, though different neurons have different levels of expression (4, 5). It is particularly concentrated in cerebellar Purkinje cells, and tends to be most abundant in perikarya and dendrites. The protein belongs to the large superfamLy of calmodulin and paravalbumin type proteins which function by binding Calcium ions. Calcium binding alters the confomation of these proteins and allow them to interact with other binding partners, the properties of which they may alter. Visinin-like protein 1 has four "EF hand" domains, which are negatively charged helix-turn-helix peptides which are responsible for Calcium binding. Visinin-like protein 1 is 191 amino acids in size and has a molecular weight on SDS-PAGE of 22 kDa. The protein has recently been suggested to be a useful biomarker of Alzheimer's disease and traumatic brain injury (6, 7, 8).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant Visinin-like protein 1 expressed and purified from E. coli.
Applications:
ICC,IHC-Frozen,WB
Clone number:
2D11
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:500 - 1:1,000 is recommended for WB. A dilution of 1:500-1:1,000 is recommended for IHC and ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a 22 kDa band by Western blot on bovine cerebellum lysate. It has also been used successfully for immunocytochemistry.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Ubiquilin 2 Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Ubiquilin 2 (also known as PLIC2 and Chap1) is a member of the ubiquilin protein family, which regulate the degradation of cellular proteins through proteasome or autophage-like pathways (1, 2, 3). Humans have four ubiquilin genes, each encoding a separate protein referred to as Ubiquilin 1, 2, 3 and 4. All ubiquilins contain an N-terminal ubiquitin-like (UBL) domain and a C-terminal ubiquitin-associated (UBA) domain, while the central part of the molecules are highly variable. The UBL domains bind subunits of the proteasome, and the UBA domains binds to polyubiquitin chains that are typically conjugated onto proteins marked for proteosomal degradation (1). Ubiquilin 2 has a unique region close to the C terminus containing 12 PXX tandem collagen like repeats, where P is proline and X is most cases valine, glycine, isoleucine or threonine. Teepu Siddique and his collaborators have identified mutations in the ubiquilin 2 gene leading to protein point mutations which were important contributors to several forms of amyotrophic lateral sclerosis (ALS) and Frontotemporal lobar degeneration (FTLD). Interestingly, these mutations involved alterations in proline residues in the PXX repeat region (P497H, P497S, P506T, P509S and P525S, ref. 4). Recently, the Lee and Trojanowski group investigated C9orf72 hexanucleotide expansion and ubiquilin 2 pathology in patients with ALS and FTLD by genetic analysis and immunohistochemistry and found distinct ubiquilin 2 pathology in ALS and FTLD-TDP with C9orf72 expansion (5). C9orf72 hexonucleotide expansion is the most commmon cause to date of familial ALS and FTLD (6, 7). Ubiquilin 2 protein is of different molecular size in mouse and human, 638 and 624 amino acids respectively. As a result the mouse protein, endogenously expressed in rodent 3T3 cells, runs on SDS-PAGE and western blots slightly slower than the human protein.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human,Mouse
Immunogen:
Recombinant human ubiquilin 2 expressed and purified from E. coli.
Applications:
ICC,WB
Clone number:
6H9
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:1,000 - 1:2,000 is recommended for WB. A dilution of 1:500-1:1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
In primary mouse neuron and glia cell culture, endogenous ubiquilin 2 appears as a weak band at 68 kDa in all tranduced and non-transduced cells, indicating low endogenous expression of mouse ubiquilin 2. Strong bands are seen in cells transduced with human wild type or mutant ubiquilin 2. Small proteins which run at 50 kDa in these cells are the fragments of ubiquilin 2. Note, ubiquilin 2 runs at ~66 kDa in human Hela cells and 68 kDa in rodent 3T3 cells. The antibody has also been used successfully for immunocytochemistry.
Storage:
Aliquot and store at -20°C for up to six months after date of receipt. Avoid freeze-thaw cycles.
Mouse anti-mCherry Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
mCherry is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals). The mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28 kDa protein similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome. The original DsRed was engineered extensively in the Tsien lab to prevent it from forming tetramers and dimers and to modify and improve the spectral properties (1-3). The resulting monomeric protein is useful for applications such as Foerster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). Several further cycles of mutation, directed modification and evolutionary selection produced mCherry, which is monomeric and has an excitation maximum at 587 nm and and emission maximum at 610 nm (4).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Species Independent
Immunogen:
Recombinant full length mCherry expressed and purified from E. coli.
Applications:
ICC,WB
Clone number:
1C51
Antibody Isotype:
IgG2a
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:1,000 to 1:2,000 is recommended for WB. A dilution of 1:250 to 1:500 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a band at ~28 kDa corresponding to intact full-length mCherry by Western blot on HEK293 cells transfected with mCherry vector. It has also been used successfully for immunocytochemistry.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Lysosomal associated membrane protein 1 (LAMP1) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
LAMP1 (Lysosomal Associated Membrane Protein 1, also known as CD107a, lysosomal associated membrane glycoprotein 1, LGP120 and LAMPA) is a protein primarily associated with the lysosomal membrane. In a typical cell LAMP1 is associated with spherical vesicles located next to the nucleus and the microtubule organizing center (1). LAMP1 is found on the cell surface of lymphocytes undergoing degranulation, a process in which cytoplasmic vesicles fuse with the plasma membrane, and this phenomena resulted in discovery of LAMP1 as a CD protein. The LAMP1 protein has a large N-terminal region which is inside the lysosome, hence topologically external to the cell, which is often referred to as the lumenal domain (2). The lumenal domain consists of two homologous globular segments separated by a proline rich sequence. Next there is a single membrane spanning domain and a short 11 amino acid C-terminal cytoplasmic tail. This tail region contains, at the extreme C-terminus, a so-called YXXI motif which is responsible for the sorting of the intact molecule to the endosome and lysozome, where Y = tyrosine, I = isoleucine and X = almost any amino acid (3). This motif is found in several other lysosomal proteins, where it functions in the same way. There are 417 amino acids in the human LAMP1 molecule, giving a native molecular weight of 44.8 kDa. However the N-terminal lumenal segment of LAMP1 is very heavily and variably glycosylated due to the presence of 18 N-linked glycosylation sites, so that on SDS-PAGE and on Western blots the protein runs as a diffuse band at 90-120 kDa. Antibodies to LAMP1 are therefore excellent markers of lysosomes in mammalian cells, though some LAMP1 may also be seen on late endosomes and on the plasma membrane.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant LAMP1 expressed and purified from E. coli.
Applications:
ICC,WB
Clone number:
LAMP1
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:2,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Lysosomal Associated Membrane Protein 1, also known as CD107a, lysosomal associated membrane glycoprotein 1, LGP120 and LAMPA
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a diffuse band at ~90 kDa to 120 kDa by Western blot on HeLa cell extract. It has also been used successfully for immunocytochemistry.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Growth associated protein 43 (GAP-43) Monoclonal Antibody (Unconjugated), suitable for WB, ICC, FC.
Background Info:
GAP43 is very abundant protein which is found concentrated in neurons. One group discovered it as one of three proteins which becomes unregulated during the regeneration of the toad optic nerve (1). Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 were independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14 kDa. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Mouse,Rat
Immunogen:
C-terminal peptide of rat and mouse GAP43, which is KEDPEADQEHA, to which an N terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein.
Applications:
FC,ICC,WB
Clone number:
GAP43
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Flow Cytometry. A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:5,000 is recommended for IC. Use 2ug/10^6 cells for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
protein F1, neuromodulin, neural phosphoprotein B-50, axonal membrane protein GAP-43, calmodulin-binding protein P-57
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a 43 kDa band by Western blot on rat spinal cord lysate. It has also been used successfully for immunocytochemistry.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Doublecortin (DCX) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Doublecortin (DCX, also known as Doublin, Lissencephalin-X, DBCN and Lis-X) was originally discovered since defects in the gene encoding it are causative of X-linked lissencephaly, a rare group of brain malformations resulting in a smooth cerebral cortex caused by aberrant neuronal migration during development (1,2). The name Doublecortin comes from the unusual layering of the cortex in this form of lissencephaly, which appears to have a second deep cortical layer of neurons. This layer consists of neurons which did not migrate from the subventricular zone to the normal cortical layer. Patients with this defect suffer from seizures and mental retardation. Four proteins encoded by the DCX produce bands of about 35 kDa and 45 kDa on Western blots. The 45 kDa form is known as Lis-XA while the smaller forms are generated by alternate transcription, are all missing the first 81 amino acids of Lis-XA, and are referred to as Lis-XB, Lis-XC, Lis-XD. There are minor amino acid sequence differences between these three smaller isoforms. All of these proteins contain two so-called Doublecortin domains, each about 90 amino acids long, which are believed to function in binding to microtubules, a C-terminal serine and proline rich region which may become phosphorylated in vivo. DCX is expressed very early in neuronal development, as neuroblasts become post-mitotic, but is lost as neurons mature. Developing neurons start to lose DCX expression about the time that they begin to express NeuN. Antibodies to DCX can be used to see if neurogenesis is taking place.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Rat
Immunogen:
Full length recombinant human Lis-A isoform of Doublecortin purified from E. coli.
Applications:
ICC,WB
Clone number:
30
Antibody Isotype:
IgG2a
Application Details:
Immunocytochemistry (ICC) and Western Blotting (WB). A dilution of 1:500-1:2,000 is recommended for WB. A dilution of 1:500-1:1,000 is recommended for ICC. The optimal dilution should be determined by the end user.
Alternative Names:
Doublin, Lissencephalin-X, DBCN and Lis-X
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with two bands at ~45 kDa and ~35 kDa which shows that the mouse anti-DCX antibody binds to an epitope in the region of DCX shared by Lis-A, and Lis-B, Lis-C and Lis-D, the C terminal 360 amino acids of Lis-A. It has also been used successfully for immunocytochemistry and is an excellent marker for developing neurons.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Actin Monoclonal Antibody (Unconjugated), suitable for WB, ICC, FC.
Background Info:
Actin is one of the most abundant and highly conserved proteins of eukaryotes. Mammalian actins are the product of six different genes with differing distribution patterns in cell types and in tissues. The molecular weight of all six proteins is 42 kDa, and one or more actins is found in essentially every type of crude cellular and tissue extract. As a result antibodies to actin are widely used as in western blotting standards. These can be used to verify that the various steps of the western blotting procedure have been performed correctly. In addition, actin is regarded as a "house keeping" protein which is generally not altered much in expression as a result of experimental manipulations. So quantitation of the actin band on the western is used as a standard against with the band density of other proteins can be compared. The monoclonal binds all six actin isotypes (ACTA1, ACTA2, ACTC1, ACTB, ACTG1 and ACTG2) very strongly on western blots. It is a very effective blotting standard which can work on any cell type or tissue extract. It also works in immunocytochemical experiments, binding strongly and cleanly to filopodia, membrane ruffles and stress fibers, all known to be rich in actin.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Horse,Human,Pig,Rat
Immunogen:
Actin prepared from bovine brain.
Applications:
FC,ICC,WB
Clone number:
5J11
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Flow cytometry. A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500 - 1:1,000 is recommended for IC. Use 2 ug/10^6 cells for Flow cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a 42 kDa band by Western blot on a crude extract from HeLa cells. It has also been used successfully for immunocytochemistry. It reacts across a broad range of mammalian species.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Microtubule Associated Protein 2 (MAP2) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one 'a' subunit and one 'b' tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2 a and MAP2 b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Rat
Immunogen:
High molecular MAP protein preparation derived from bovine brain
Applications:
ICC,IHC-Frozen,WB
Clone number:
5H11
Antibody Isotype:
IgG
Application Details:
Immunohistochemistry (IHC), Immunocytochemistry (ICC) and Western Blotting (WB). A dilution of 1:1,000 - 1:5,000 is recommended for IHC and ICC, and 1:5,000-1:10,000 is recommended for WB. The optimal dilution should be determined by the end user.
Alternative Names:
Microtubule-associated protein 2; MAP-2; Mtap2;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; Rat; Mouse;
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Tyrosine hydroxylase (TH) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ICC, IP, FC.
Background Info:
Tyrosine hydroxylase is an excellent marker for dopaminergic and noradrenergic neurons. Tyrosine hydroxylase (a.k.a. tyrosine 3-monooxygenase) is the enzyme responsible for catalyzing the conversion of the amino acid L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA). L-DOPA is a precursor for dopamine, which, in turn, is a precursor for the important neurotransmitters norepinephrine (noradrenaline) and epinephrine (adrenaline). Tyrosine hydroxylase catalyzes the rate limiting step in this synthesis of catecholamines. In humans, tyrosine hydroxylase is encoded by the TH gene, and the enzyme is present in the central nervous system (CNS), peripheral symphatic neurons and the adrenal medulla. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing. Tyrosine hydroxylase, phenylalanine hydroxylase and tryptophan hydroxylase together make up the family of aromatic amino acid hydroxylases (AAAHs).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized, dry powder.
Host Animal:
Mouse
Species Reactivity:
Chicken,Frog,Horse,Human,Mouse,Primate,Rat,Sheep,Vole,Zebra Fish
Immunogen:
Tyrosine Hydroxylase purified from PC12 cells
Applications:
FC,ICC,IHC-Frozen,IHC-Paraffin-embedded,IP,WB
Clone number:
LNC, LNC1, LNC-1
Antibody Isotype:
IgG1, kappa
Application Details:
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry/paraffin embedded IH(P), Immunoprecipitation (IP), Immunofluorescence (IF), Flow cytometry (FC).WB: 1:1000 -1:2000, SDS reduced samples. Detects a 59-61kDa protein. Rat Brain lysates is a suitable control. IHC/IH(P): Reacts in formalin fixed paraffin embedded tissues with HIER antigen recovery. Typical dilution is 1:100-1:200 depending upon incubation time and detection method used. IF: 1:200-1:1000, 4% PFA fixed tissues/cells permeabilized with 0.1-0.4% triton X-100; also works in fresh frozen and acetone fixed tissues/cells.IP: 1:100, immobilized on protein A beads, Fleming-Jones et al (1995) J. Protein Chemistry 14(5):275-282.FC: Fixed, permeabilized dopaminergic nerve terminals from rat striatum, {Wolf, ME, Kapatos, G (1989) The Journal of Neuroscience, January 1989, 9(l): 108-114; Wolf ME, Zigmond, MJ, Kapatos, G (1989) J. Neurochemistry 53(3):879-885}.
Alternative Names:
LNC-1; LNC1; TH monoclonal
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Kapatos G., Kemski V., and Geddes T. (1989) Dopamine neurons in monolayer culture as a model system for the study of tyrosine hydroxylase, in Pteridines and Biogenic Amines in Neuropsychiatry, Pediatrics and Immunology (Levine R. A., KuhnD. M., Milstien S., and Curtius H-C., eds), pp. 243-258. Lakeshore Publishers, Grosse Pointe, Michigan.
Specificity:
Clone LNC 1 recognizes an epitope on the outside of the regulatory N-terminus. The clone detects a protein of approximately 59-61 kDa by Western blot and reduced SDS-PAGE. The clone does not react with dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT) by western blots. Chicken, Frog, Horse, Human, Monkey, Mouse, Vole, Sheep, Zebrafish other species not yet tested
Storage:
After reconstitution keep aliquots at -20 ° to -70°C for a higher stability. At 2-8°C keep up to one week, insulated, protected from light; use sterile methods and pipettes. Highly purified glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. Keep tightly closed when not in use and protected from light
Purification:
Unpurified ascites fluid, diluted with PBS containing 3% BSA, no preservatives.
The amyloid beta peptide is derived from the cleavage of the Amyloid precursor protein (APP) and varies in length from 39 to 43 amino acids. However, the form(s) of amyloid-beta peptide (A? associated with the pathology characteristic of Alzheimer's disease (AD) remains unclear. In particular, the neurotoxicity of intraneuronal A? accumulation is an area of considerable research and controversy principally because antibodies thought to be specific for A? have been shown to actually detect intraneuronal APP and not A? exclusively.<br /><br />MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to A? residues 1-4 as demonstrated by biochemical and immunohistochemical analyses (IHC), and is highly specific just to amyloid beta peptide.<br /><br />MOAB-2 did not detect APP or APP-CTFs in cell culture media/lysates (HEK-APPSwe or HEK APPSwe/BACE1) or in brain homogenates from transgenic mice expressing 5 familial AD (FAD) mutation (5xFAD mice). <br /><br />Using IHC on 5xFAD brain tissue, MOAB-2 immunoreactivity co-localized with C-terminal antibodies specific for A?40 and A?42. MOAB-2 did not co-localize with either N- or C-terminal antibodies to APP. In addition, no MOAB-2-immunreactivity was observed in the brains of 5xFAD/BACE-/- mice, although significant amounts of APP were detected by N- and C-terminal antibodies to APP, as well as by 6E10.<br /><br />In both 5xFAD and 3xTg mouse brain tissue, MOAB-2 co-localized with cathepsin-D, a marker for acidic organelles, further evidence for intraneuronal A?, distinct from A? associated with the cell membrane. MOAB-2 demonstrated strong intraneuronal and extra-cellular immunoreactivity in 5xFAD and 3xTg mouse brain tissues.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized, from a Protein A purified preparation in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% sodium azide, 0.1% trehalose, pH 7.2; contains 0.01% sodium azide as a preservative.
Host Animal:
Mouse
Species Reactivity:
Human,Rat
Immunogen:
Recombinant human amyloid beta protein 42 (A?42): DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry/paraffin embedded IH(P), Immunoprecipitation (IP), Immunofluorescence (IF), ELISA.<br><br>Antibody has been tested in WB using purified synthetic beta-amyloid preparations and from transgenic mouse brain formic acid extracts (see figure 1). Formic acid extraction/concentration is required for western blot detection from extracts. MOAB-2 antibody is specific for beta-amyloid and does not detect APP. Suggested dilution of 1:2000-1:5,000 for WB, standard ECL detection systems. <br><br>Tissue samples for the detection of beta-amyloid should be prepared as detailed in K.L. Youmans et al. {Journal of Neuroscience Methods 196 (2011) 51-59} for best results. Detection of beta-amyloid 40/42 in direct westerns can be difficult; Dot-blots of prepared samples are recommended as detailed in Youmans. KL et al 2012. <br><br>IR or fluorescent detection systems not yet tested, they but are expected to work well with higher primary antibody dilutions because of the increased sensitivity of the detection methods.<br><br>Suggested dilutions for IHC are 1:50-1:1,000. Fresh frozen, 4% paraformaldehyde fixed frozen, or formalin fixed paraffin embedded tissues are all suitable. Optimal dilutions must be determined by the end user. Antigen retrieval is required in fixed tissues for optimal staining.<br><br>Antibody was tested on 4% paraformaldehyde/0.1% glutaraldehyde fixed frozen tissue from 3xTg and 5xFAD mice. MOAB-2 antibody detects intraneuronal and extracellular beta-amyloid in IHC and does not detect APP {Youmans KL et al 2012}.<br><br> The antibody also reacts with archival formalin-fixed, paraffin-embedded tissue samples with antigen Heat Induced Epitope Retrieval (HIER): Recommended Citrate, pH 6.0 buffer for HIER. Signal was weak without antigen retrieval. Immunoreactively was expressed in intraneural-amyloid deposition (plaque) in Alzheimer's brain. MoAB-2 was found to be extremely clean and with an excellent signal to noise ratio with no neuro-cellular diffusive staining.<br><br>In addition MOAB-2 demonstrated no significant differences in A-beta detection using paraffin fixed, free-floating sections {Youmans KL et al 2012}. Formic acid (FA) treatment resulted in optimal detection of both intraneuronal and extracellular A-beta compared to without FA (incubated in 88% FA 8 min, Youmans KL et al 2012). Free floating tissue sections were permeabilized in TBS containing 0.25% Triton X-100 (TBSX; 3 x 10 min), blocked with 3% horse serum in TBSX (3 x 10 min) followed by 1% horse serum in TBSX (2 x10 min) and incubated with appropriate primary antibodies diluted in TBSX containing 1% horse serum overnight. See Youmans KL et al 2012 for full IH(P) protocol and method details.<br><br> For IF, suggested dilution is 1:100-1:500. The antibody was tested on 4% PFA fixed frozen tissue. Fixed tissues were washed in TBS (3 x 10 min), then incubated in 88% FA (8 min), and then permeabilized in TBSX (3 x 10 min), and blocked in TBSX containing 5% bovine serum albumin (BSA; 1 hr). Sections were subsequently incubated with appropriate primary antibodies diluted in TBSX containing 2% BSA overnight on an oscillatory rotator. Detection was via fluorescently labelled absorbed secondary antibodies {Youmans KL et al 2012}.<br><br>For IP, the suggested dilution is 1:200 to 1:1,000 for labeled beta-amyloid using Protein A/G conjugated beads as the capture vehicle {Youmans KL et al 2012}.<br><br>In an ELISA, a dilution of 1:50-1:1000 is suggested. The antibody has been tested in ELISAs on synthetic beta-amyloid and tissue homogenates from beta-amyloid-Tg mice. Biosensis recommends optimal dilutions/concentrations should be determined by the end user for all applications. Dilutions provided are only meant to serve as a basic guide.
Alternative Names:
Beta-APP42; Beta-APP40; Beta-amyloid protein 42; Beta-amyloid protein 40; ABPP; APPI; Amyloid beta A4 protein;MOAB2;MOAB-2; Alzheimer's antibody;AB40;AB42;abeta
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Setti, S.E. et al. (2022) Assessment of sex-related neuropathology and cognitive deficits in the Tg-SwDI mouse model of Alzheimers disease. Behave Brain Res. 428:113882. Application: IHC. Sil, A. et al. (2022) Sex Differences in Behavior and Molecular Pathology in the 5XFAD Model. J Alzheimers Dis. 85(2):755-778. Application: WB. Sarkar, S. et al. (2020) Modification of methods to use Congo-red stain to simultaneously visualize amyloid plaques and tangles in human and rodent brain tissue sections. Metab Brain Dis. [Epub ahead of print]. Application: IHC. Cuevas, E. et al. (2019) Amyloid Beta 25-35 induces blood-brain barrier disruption in vitro. Metab Brain Dis. [Epub ahead of print]. Application: ICC/IF. Schmued, L. et al. (2019) High Contrast and Resolution Labeling of Amyloid Plaques in Tissue Sections from APP-PS1 Mice and Humans with Alzheimer's Disease with the Zinc Chelator HQ-O: Practical and Theoretical Considerations. Curr Alzheimer Res. 16(7):577-586. Application: IHC/IF. Hui, L. et al. (2019) Acidifying Endolysosomes Prevented Low-Density Lipoprotein-Induced Amyloidogenesis. J Alzheimers Dis. 64(1):393-410. Application: ICC/IF. Koss, DJ. et al. (2018) Distinctive temporal profiles of detergent-soluble and -insoluble tau and A? species in human Alzheimer's disease. Brain Res. [Epub ahead of print]. Application: WB, dot blot. Zhao, Y. et al. (2018) TREM2 Is a Receptor for _-Amyloid that Mediates Microglial Function. Neuron. 97(5):1023-1031. Application: IHC, free-floating cryostat sections Zhu, B. et al. (2017) ER-associated degradation regulates Alzheimer's amyloid pathology and memory function by modulating _-secretase activity. Nat Commun. 8(1):1472. Application: IHC Huang, TY. et al. (2017) SORLA attenuates EphA4 signaling and amyloid _-induced neurodegeneration. J Exp Med. pii: jem.20171413. [Epub ahead of print]. Application: IHC Felecia, M. et al. (2017) Peripheral Inflammation, Apolipoprotein E4, and Amyloid-_ Interact to Induce Cognitive and Cerebrovascular Dysfunction. ASN Neuro. 9(4):1759091417719201. Application: IHC/IF Thomas, R. et al. (2016) Epidermal growth factor prevents APOE4 and amyloid-beta-induced cognitive and cerebrovascular deficits in female mice. Acta Neuropathol Commun. 4(1):111 Application: IHC Koster, KP. et al. (2016) Epidermal growth factor prevents oligomeric amyloid-_ induced angiogenesis deficits in vitro. J Cereb Blood Flow Metab. [Epub ahead of print] Application: IF Loffler, T. et al. (2016) Decreased Plasma A? in Hyperlipidemic APPSL Transgenic Mice Is Associated with BBB Dysfunction. Front. Neurosci. Application: IF Kobro-Flatmoen, A. et al. (2016) Reelin-immunoreactive neurons in entorhinal cortex layer II selectively express intracellular amyloid in early Alzheimer's disease. Neurobiology of Disease. 93:172-183. Application: IHC Tai, LM. et al. (2016) The role of APOE in cerebrovascular dysfunction. Acta Neuropathol. 131(5):709-23. Application: IF Kim, YH. et al. (2015) A 3D human neural cell culture system for modeling Alzheimer's disease. Nat Prot. 10(7):985-1006. Application: WB Condello, C. et al. (2015) Microglia constitute a barrier that prevents neurotoxic protofibrillar A?42 hotspots around plaques. Nat Commun. 6:6176. Application: IF Iulita MF et al (2014) Studying Alzheimer's Disease Pre-clinical Stages: Insights from Down's Syndrome and Transgenic Animal Models. PhD Thesis Application: IHC/IF Iulita MF et al (2014) Intracellular Abeta pathology and early cognitive impairments in a transgenic rat model overexpressing human amyloid precursor protein: a multidimensional study. Acta Neuropathol Commun. 6:61. Application: IF, IH Smith BR et al (2014) Neuronal inclusions of alpha-synuclein contribute to the pathogenesis of Krabbe disease. J Pathol. Apr;235(5):509-21. Application: IF
Specificity:
MOAB-2 detects preparations enriched in U-, O-, F-A?42, and U-A?40 by dot-blot, and is thus a pan-specific A? antibody. However, MOAB-2 is selective for the more neurotoxic A?42 compared to A?40. Indeed, MOAB-2 demonstrated a titration against antigen concentration, and detects A?40 at 2.5 pmol but U-, O- and FA?b42 at antigen concentrations as low as ~ 0.1 pmol {Youmans. KL et al 2012}. MOAB-2 does not detect APP (Amyloid precursor protein). Human, Rat, other species not yet tested.By Dot blot, MOAB-2 detected rat A?40 and human A?40, albeit with less affinity than for A?42. {Youmans. KL et al 2012}
Storage:
After reconstitution keep aliquots at -20 ° to -70°C for a higher stability. At 2-8°C keep up to one week, insulated, protected from light; use sterile methods and pipettes. Highly purified glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles. Keep tightly closed when not in use and protected from light.
Purification:
This product is a Protein A purified mouse IgG2b in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% sodium azide, pH 7.2.
Mouse anti-Splicing factor 3B subunit 4 (SF3B4) Monoclonal Antibody (Unconjugated), suitable for WB, ICC, FC.
Background Info:
SF3B4 is one of 8 subunits of splicing factor SF3B. SF3B4 is ubiquitously expressed in the nuclei of eukaryotic cells, although it migrates into the cytoplasm of dividing cells.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Full length recombinant human SF3B4 which was expressed in and purified from E. coli.
Applications:
FC,ICC,WB
Clone number:
3A1
Antibody Isotype:
IgG2b
Application Details:
WB, ICC, Flow Cytometry. Recommended dilution of 1:500-1:2,000 for ICC. In WB using chemiluminescence it can be used at dilutions of 1:1,000 or lower. The protein runs on SDS-PAGE gels at an apparent molecular weight of 49 kDa. Use 2 ug/10^6 cells for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
SAP49; splicing factor 3b subunit 4; 49 kDa SAP49; spliceosome-associated protein 49; U2 snRNP; Hsh49; MGC108282; SF3B4; SF3b50;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human SF3B4 ; Bovine; Porcine; Mouse; Rat; expected to react with other species due to sequence homology
Storage:
Aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Mouse anti-Spectrin alpha chain, non-erythrocytic 1 Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC, FC.
Background Info:
Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. The Spectrins form into dimers and further into tetramers of alpha and beta subunits (Ref: Entrez Gene). The alpha-II subunit is widely expressed in tissues but, in the nervous system, is found predominantly in neurons.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
This antibody was raised against a recombinant construct containing the seventh, eight and ninth repeats (amino acids 676-1043) of human alpha-II Spectrin. The 9th spectrin repeat also includes a Src-homology 3 domain. This construct was expressed in and purified from E. coli.
Applications:
FC,ICC,IHC-Frozen,WB
Clone number:
3D7
Antibody Isotype:
IgG1
Application Details:
WB, ICC, IHC and FC. Recommended dilution of 1:1,000-1:2,000 for ICC and IHC, and 1:5,000-10,000 for WB. The protein is seen as a major band at 240 kDa depending on the species. For Flow Cytometry, use ~ 2 ?g antibody per ~10^6 cells. Optimal concentrations/dilutions should be determined by the end-user.
Mouse anti-Galectin-3 (Gal-3) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Galectin 3 is a lectin with carbohydrate recognition domains (CRD) which bind -galactoside. It is a multifunctional protein expressed both on the cell surface, cytoplasm and nucleus and appears to have roles in specific carbohydrate binding and in the regulation of mRNA splicing.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Full length recombinant Galectin-3 expressed in and purified from E. coli.
Applications:
ICC,WB
Clone number:
5C21
Antibody Isotype:
IgG1
Application Details:
WB, ICC. Suggested dilution of at least 1:1,000 for ICC. Suggested dilution of 1:2,000 or lower for WB. Optimal concentrations/dilutions should be determined by the end-user.
Mouse anti-Parkinson disease protein 7 (PARK7) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Protein DJ-1 has many roles including protecting cells against oxidative stress and cell death (Ref: SwissProt). Mutations in the DJ-1 gene have been associated with rare forms of autosomal recessive early-onset Parkinson's disease.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Full length recombinant human DJ-1 expressed in and purified from E. coli.
Applications:
ICC,WB
Clone number:
4H4
Antibody Isotype:
IgG1, kappa
Application Details:
WB, ICC. Suggested dilution of at least 1:500 for ICC. Dilutions of 1:5,000 or lower is recommended for WB. This antibody reveals a prominent ~21 kDa band and stains mainly in cytoplasm of tissue culture cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Oncogene DJ1; Parkinson disease protein 7; PARK7; DJ-1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a 21 kDa band by Western blot on whole HeLa cell lysate. It has also been used successfully for immunocytochemistry. Does not react with rat and mouse DJ-1 protein on western blots.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-14-3-3 protein eta Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
14.3.3 protein eta or 14.3.3 binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (Ref SwissProt). 14.3.3 protein eta is widely expressed as both homodimers and heterodimers and are concentrated in the nervous system. High concentrations of 14.3.3 protein eta have been linked to Creutzfeld Jacob Disease, Parkinson's Disease and early-onset schizopherenia.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Full length recombinant 14.3.3 protein ETA expressed in and purified from E. coli.
Applications:
ICC,IHC-Frozen,WB
Clone number:
3G12
Antibody Isotype:
IgG
Application Details:
WB, ICC, IHC. Suggested dilution of 1:500-1:1,000 for IHC and ICC. Suggested dilution of 1:1,000-1:5,000 for WB. A suitable control tissue is rat spinal cord or peripheral nerve homogenate.
Alternative Names:
14.3.3 ; Protein AS1; YWHAH; YWHA1; tyrosine 3-monooxygenase; tryptophan 5-monooxygenase activation protein 1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human 14-3-3 ETA protein
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Lipid phosphate phosphohydrolase 3 (LPP3) Monoclonal Antibody (Unconjugated), suitable for WB, FC.
Background Info:
Lipid phosphate phosphohydrolase 3 (LPP3) is a member of the phosphatidic acid phosphatase (PAP) family. LPP3 catalyzes the conversion of phosphatidic acid to diacylglycerol. In addition it hydrolyzes lysophosphatidic acid, ceramide-1-phosphate and sphingosine-1-phosphate (Ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.4
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
A synthetic peptide from human LPP3 (179-196 aa) conjugated to KLH.
Applications:
FC,WB
Clone number:
7H7D3
Antibody Isotype:
Mix of IgG1, IgG2a & IgG2b
Application Details:
Western Blotting (WB), Flow cytometry (FACS) and Immunohistochemistry (IHC). For WB, the recommended concentration is 2-3 µg/mL. For IHC, this antibody has been shown to work on formalin-fixed, paraffin-embedded tissue samples with heat-induced antigen retrieval. The recommended concentration is 0.5-2 µg/mL. For FACS, the recommended concentration is 2.0 µg/mL. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Lipid phosphate phosphohydrolase 3; PAP2-beta; Phosphatidate phosphohydrolase type 2b; Phosphatidic acid phosphatase 2b; PAP-2b; PAP2b; Vascular endothelial growth factor and type I collagen-inducible protein; VCIP; PPAP2B;LPP3
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Mueller PA (2016) PPAP2B expression limits lesion formation in murine models of atherosclerosis. Doctoral Dissertation. Species: Mouse. Application: IHC. Reschen ME, Gaulton KJ, Lin D, Soilleux EJ, Morris AJ, Smyth SS and O'Callaghan CA (2015) Lipid-induced epigenomic changes in human macrophages identify a coronary artery disease-associated variant that regulates PPAP2B Expression through Altered C/EBP-beta binding. PLoS Genet. 2015 Apr 2;11(4):e1005061. Species: Human. Application: IHC with heat-induced antigen retrieval. Humtsoe JO, Liu M, Malik AB and Wary KK (2010) Lipid phosphate phosphatase 3 stabilization of beta-catenin induces endothelial cell migration and formation of branching point structures. Mol Cell Biol. Apr;30(7):1593-606. Species: Human. Application: WB and IHC.
Specificity:
Confirmed by over-expression of human LPP3 cDNA. Human
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
Recombinant full length human Ubiquitin C Terminal Hydrolase 1 (UCHL1) purified from E. coli.
Applications:
ICC,IHC-Frozen,WB
Clone number:
BH7
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:10,000 - 1:20,000 is recommended for WB. A dilution of 1:1,000 - 1:5,000 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord, brain, SHSY-5Y or HEK293 cell extract. Hu, Rat, Bov
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Polyubiqutin-B Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Background Info:
Ubiquitin is a highly conserved 76 amino acid protein with an estimated molecular weight of 8.56 kDa which has a central role in regulated protein degradation. It is a protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Several types of polymeric chains can be formed depending on the lysine used for the assembly. Attachment to proteins as a polymer leads to their degradation by the 26S proteosome; a complex, multicatalytic cytosolic and nuclear protease. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatic structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats of which differ between species and strains. In some species there is a final amino-acid after the last repeat, here in bovine a Cys. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,C. elegans,Chicken,Drosophila,Human
Immunogen:
Raised against purified ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin.
Applications:
IHC-Frozen,IHC-Paraffin-embedded,WB
Clone number:
Ubi-1
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB), Immunohistochemistry - paraffin embedded tissue (IH-P) and ELISA. Suggested dilution for WB is 1:500-1,000. This antibody can be used on mildly fixed histological sections of human brain for studies of Alzheimer's disease. This antibody also works on paraffin embedded material. It also recognises other ubiquinated inclusion bodies such as Lewy bodies of Parkinson's disease and the Pick bodies in Pick's disease in formalin fixed tissues. Suggested dilution for IH is 1:500. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Josephs K.A. et al (2006) Atypical progressive supranuclear palsy with corticospinal tract degeneration. J Neuropathol Exp Neurol. 2006 Apr;65(4):396-405. Josephs K.A. et al (2007) Neuropathologic features of frontotemporal lobar degeneration with ubiquitin-positive inclusions with progranulin gene (PGRN) mutations. J Neuropathol Exp Neurol. 2007 Feb;66(2):142-51. Rudzinski L.A. et al (2008) Early onset familial Alzheimer Disease with spastic paraparesis, dysarthria, and seizures and N135S mutation in PSEN1. Alzheimer Dis Assoc Disord. 2008 Jul-Sep;22(3):299-307. Josephs K.A. et al (2009) Evaluation of subcortical pathology and clinical correlations in FTLD-U subtypes. Acta Neuropathol. 2009 Sep;118(3):349-58.
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody detects ~8.5 kDa Ubiquitin. Hu, Bov, Chk, Drosophila, and C. elegans
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-TAR DNA-binding protein 43 (TDP-43) Monoclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
Background Info:
TAR DNA-binding protein 43 (TDP43) is a DNA and RNA-binding protein which regulates transcription and splicing (ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
This antibody was raised against recombinant full length human his-tagged TDP43 which was expressed in E. coli and purified by nickel affinity.
Applications:
ICC,IHC-Frozen,WB
Clone number:
3H8
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:1,000 - 1:5,000 is recommended for WB and IHC. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
TAR DNA-binding protein 43; TDP-43; TARDBP; TDP43;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody detects ~43 kDa TDP43 protein on crude extract of mouse brain nuclear fraction. Human and Rodent. Predicted to react with other mammalian tissue.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Alpha-synuclein Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. FUNCTION: May be involved in the regulation of dopamine release and transport. Soluble protein, normally localized primarily at the presynaptic region of axons, which can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation. TISSUE SPECIFICITY: Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.SUBUNIT: Soluble monomer which can form filamentous aggregates. Interacts with UCHL1. Interacts with phospholipase D and histones. SUBCELLULAR LOCATION: Cytoplasm. Membrane. Nucleus. Note=Membrane-bound in dopaminergic neurons. Also found in the nucleus. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. Additional isoforms seem to exist.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Recombinant full length human alpha synuclein expressed and purified from E. coli
Applications:
ICC,WB
Clone number:
3H9
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB) and Immunocytochemistry (IC/IF). A dilution of 1:1,000 - 1:5,000 is recommended for WB. A dilution of 1:500-3,000 is recommended for IC/IF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody detects ~14-15 kDa alpha synuclein protein. The epitope for 3H9 is in the region 61-95 which correspond to the Non-Amyloid beta Component of Alzheimer's disease amyloid (NAC) region. 3H9 will also bind human alpha-synuclein containing the A30P and A53T mutations. Human, horse, cow, pig, chicken, rat, mouse. Predicted to react with other mammalian tissue because of highly conserved nature of the protein.
Storage:
After reconstitution of lyophilized antibody, divide into single use aliquots and store at -20-80°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Peripherin Monoclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
Background Info:
Peripherin is a class-III neuronal intermediate filament protein found in certain classes of neuron, most of which are located in the peripheral nervous system.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Cat,Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant full length rat Peripherin protein expressed in and purified from E.coli
Applications:
ICC,IHC-Frozen,WB
Clone number:
7C5
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A concentration of 0.5 - 2 µg/mL is recommended for WB. A concentration of 1-5 µg/mL is recommended for IC and IH. This antibody performs well on aldehyde fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Peripherin; Prph; Prph1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Sekerkova G. et al (2008) Espin actin-cytoskeletal proteins are in rat type I spiral ganglion neurons and include splice-isoforms with a functional nuclear localization signal. J Comp Neurol. 2008 Aug 20;509(6):661-76.
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody detects ~57 kDa Peripherin protein. Human, mouse, feline. Predicted to react with other mammalian tissue.
Storage:
Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Nuclear pore complex protein Nup107 Monoclonal Antibody (Unconjugated), suitable for ICC.
Background Info:
The Nuclear Core Complex (NPC) acts as a gateway for macromolecular traffic between the cytoplasm and the nucleus.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat,Yeast
Immunogen:
Yeast nuclear preparation
Applications:
ICC
Clone number:
39C7
Antibody Isotype:
IgG1
Application Details:
Immunocytochemistry (ICC). A dilution of 1:50-1:500 is recommended for IC. This antibody does not work well for Western Blotting. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Mouse anti-Neurofilament medium (NF-M) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC, FC.
Background Info:
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Cat,Chicken,Human,Mouse,Pig,Rat
Immunogen:
Raised against a recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli. The epitope is localized to within the last 56 amino acids at the extreme C-terminus of rat NF-M, the so-called KE segment which is highly conserved between NF-M molecules from different species.
Applications:
FC,ICC,IHC-Frozen,WB
Clone number:
3H11
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) and Flow Cytometry. A dilution of 1:2,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:5,000 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Felitsyn N. et al (2008) The heme precursor delta-aminolevulinate blocks peripheral myelin formation. J Neurochem. 2008 Sep;106(5):2068-79.
Specificity:
Specifically recognizes the medium neurofilament subunit NF-L in WB. Hu, Rat, Ms, Fel, Bov, Por, Chk
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Neurofilament light (NF-L) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC, FC.
Background Info:
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Cat,Chicken,Human,Mouse,Pig,Rat
Immunogen:
Enzymatically dephosphorylated full length pig NF-L protein. The antibody binding epitope has been mapped to a short peptide in the C-terminal tail region of the molecule within the sequence YYTSHVQEEQIEVEETIEA, amino acids 441-460 of the human sequence.
Applications:
FC,ICC,IHC-Frozen,WB
Clone number:
DA2
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) and Flow Cytometry (2 ug per 10^6 cells). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:100 - 1:500 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Chicken,Other Mammals (Predicted),Pig,Rat
Immunogen:
Full length native protein (purified) from Pig spinal cord.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Clone number:
NAP4
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) and Flow Cytometry (2 ug/10^6 cells). Suggested dilution for WB of 1:5,000-10,000. This antibody recognises NF-H in frozen sections, tissue culture and in formalin-fixed sections. Suggested dilution for IC is 1:500-1,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Boylan K. et al (2009) Immunoreactivity of the phosphorylated axonal neurofilament H subunit (pNF-H) in blood of ALS model rodents and ALS patients: evaluation of blood pNF-H as a potential ALS biomarker. J Neurochem. 2009 Dec;111(5):1182-91. Rangaraju S. et al (2009) Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging. Aging Cell. 2009 Apr;8(2):178-91.
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody recognises phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related KSP sequences found in subunit NF-M. Chicken, Rat. Predicted to react with mammals due to sequence homology.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Nestin Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells. The molecular weight of human Nestin as determined by SDS-PAGE mobility is about 240 kDa. However the real molecular weight is considerably less than this, at 177 kDa, the disparity being likely due to the highly charged region of the C-terminal segment. Nestin is relatively poorly conserved in protein sequence across species boundaries, so that the mouse and human proteins have an overall identity of only 62%. As a result antibodies to the human protein often fail to recognize the rodent homologue and vice versa. However this antibody stains both rodent and human Nestin. Antibodies to Nestin are widely used to identify neural stem cells.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Partial segment (region 317-630 aa) of human Nestin expressed in E.coli
Applications:
ICC,WB
Clone number:
4D11
Antibody Isotype:
IgG1, kappa
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Flow Cytometry. Suggested dilution for WB is 1:1,000-5,000 and 1:250-500 for IC. Use 2 ug/10^6 cells for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Nestin; NES;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Schomann T et al. (2020) Multimodal imaging of hair follicle bulge-derived stem cells in a mouse model of traumatic brain injury. Cell Tissue Res. [Epub ahead of print]. Application: IHC/IF; Species: Mouse. Schomann T et al. (2017) Neuronal differentiation of hair-follicle-bulge-derived stem cells co-cultured with mouse cochlear modiolus explants. PLos One. 12(10):e0187183. Application: ICC/IF; Species: Mouse, Hair follicle bulge-derived neural crest-derived stem cells (HFBSCs). Gho CG et al. (2015) Isolation, expansion and neural differentiation of stem cells from human plucked hair- a further step towards autologous nerve recovery. Cytotechnology In press. Application: IF; Species: Human, Hair follicle bulge-derived neural crest-derived stem cells (HFBSCs), Keywords: Hair follicle stem cell, Regeneration, Neural crest, Neuron, Glia, Cryopreservation
Specificity:
This antibody is specific for the 240 kDa Nestin protein by WB on developing rat brain (P18) homogenate. A much weaker band at approx. 90 kDa may also be seen. This is suggested to be a breakdown product of the 240 kDa band. Human, Rodent
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Myelin basic protein (MBP) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of approximately 80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP). MBP is an 'intrinsically unstructured' protein with a high proportion (approximately 75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system. A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational. Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic. The message for myelin basic protein is selectively translocated to the ends of the cell processes. Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model. FUNCTION: Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity). SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Myelin membrane; peripheral membrane protein; cytoplasmic side. Cytoplasmic side of myelin. TISSUE SPECIFICITY: Found in both the central and the peripheral nervous system.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Rat
Immunogen:
Three peptide sequences conserved in higher verterbrate MBP protein.
Applications:
ICC,IHC-Frozen,WB
Clone number:
7G7
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC). IH(P), and Flow Cytometry (~2 ug per10^6 cells). The recommended dilution for WB is 1:5,000-10,0000 and 1:500-1,000 for IC and IH and IH(P). Material should not be over fixed; 2-3 hour post-fixing time is recommended. Long fixations can effect reactivity. In paraffin citrate acid treatment for antigen recovery is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB. This antibody stains bands around 21.5 kDa and 18.5 kDa. A suitable control tissue is rat spinal cord or peripheral nerve homogenate. The major isoforms of MBP run as a closely spaced double of 22 kDa and 18 kDa. Human, Rat
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Alpha-internexin is a ~66 kDa Class IV intermediate filament subunit expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Cat,Human,Mouse,Other Mammals,Rat
Immunogen:
Recombinant rat alpha-internexin expressed and purified from E. coli
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Clone number:
1D2
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry on paraffin sections (IHC). The recommended dilution for WB is 1:5000-10,000. The epitope recognised by this antibody is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation so this antibody is ideally suited for studies of paraffin embedded formalin fixed histological sections. The recommended dilution for IHC is 1:1,000-5,000. For IC, dilution is 1:250-500. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB. This antibody is specific for the 64-66 kDa alpha-internexin protein. Molecular weight will depend on species. Hu, Rat, Ms, Fel, and other mammals
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. GAPDH may have other roles in the activation of transcription and in the regulation of apoptosis as well as Alzheimer's disease and Huntington's disease. The immunogen used to raise this particular antibody was extensively purified pig GAPDH. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Chicken,Human,Mouse,Pig,Rat
Immunogen:
Purified pig GAPDH
Applications:
ICC,IHC-Frozen,WB
Clone number:
1D4
Antibody Isotype:
IgM
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:1,000 is recommended for WB. Human GAPDH has a predicted length of 335 residues and a MW of 36 kDa. A dilution of 1:100 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Fortun J. et al (2003) Emerging role for autophagy in the removal of aggresomes in Schwann cells. J Neurosci. 2003 Nov 19;23(33):10672-80. Felitsyn N. et al (2008) The heme precursor delta-aminolevulinate blocks peripheral myelin formation. J Neurochem. 2008 Sep;106(5):2068-79. Iskandar M. et al (2005) Copper chaperone for Cu/Zn superoxide dismutase is a sensitive biomarker of mild copper deficiency induced by moderately high intakes of zinc. Nutr J. 2005 Nov 24;4:35. Bizzozero O.A. et al (2009) Identification of major S-nitrosylated proteins in murine experimental autoimmune encephalomyelitis. J Neurosci Res. 2009 Oct;87(13):2881-9. Madorsky I. et al (2009) Intermittent fasting alleviates the neuropathic phenotype in a mouse model of Charcot-Marie-Tooth disease. Neurobiol Dis. 2009 Apr;34(1):146-54. Bertinato J. et al (2010) Decreased Erythrocyte CCS Content is a Biomarker of Copper Overload in Rats. Int J Mol Sci. 2010 Jul 2;11(7):2624-35. Rangaraju S. et al (2008) Pharmacological induction of the heat shock response improves myelination in a neuropathic model. Neurobiol Dis. 2008 Oct;32(1):105-15. Rangaraju S. et al (2009) Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging. Aging Cell. 2009 Apr;8(2):178-91.
Specificity:
The specificity of this antibody has been confirmed by WB. Human, Rat, Mouse, Bovine, Porcine, Chicken
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Glial fibrillary acidic protein (GFAP) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:5,000 is recommended for WB. Human GFAP has a predicted length of 432 residues and a MW of 50 kDa. A dilution of 1:500-1:1,000 is recommended for ICC/IHC. This antibody works well on frozen sections, cells in tissue culture and on formalin fixed histological sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Astrocyte; Glial fibrillary acidic protein; GFAP;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Kawabe K et al. (2017) Transglutaminases Derived from Astrocytes Accelerate Amyloid _ Aggregation. Neurochem Res. [Epub ahead of print]. Application: ICC (cultured rat astrocytes). Nagai T et al. (2017) Development of an in situ evaluation system for neural cells using extracellular matrix-modeled gel culture. J Biosci Bioeng. 124(4):430-8. Application: IF (artificial gel matrix). Kawabe T et al. (2017) Microglia Endocytose Amyloid _ Through the Binding of Transglutaminase 2 and Milk Fat Globule EGF Factor 8 Protein. Neurochem Res. [Epub ahead of print] Application: ICC (cultured astrocytes). Takano K et al. (2017) Inhibition of Gap Junction Elevates Glutamate Uptake in Cultured Astrocytes. Neurochem Res. [Epub ahead of print] Application: ICC (cultured astrocytes).
Specificity:
The specificity of this antibody has been confirmed by WB. Human, Rat, Mouse, Bovine, Porcine. Predicted to react with other mammalian and avian species.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Fibrillarin is part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. This means that suitably cross-reactive antibodies to Nop1p/fibrillarin such as this antibody can be used to reveal nucleoli and study fibrillarin/Nop1p in all eukaryotes and archea tested to date. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
C. elegans,Drosophila,Human,Other Mammals (Predicted),Rat,Yeast
Immunogen:
Yeast nuclear preparations. Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae).
Applications:
ICC,WB
Clone number:
38F3
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC). A dilution of 1:2000 (cell lysates) to 1:10000 (nuclear fractions) is recommended for WB of yeast protein samples followed by chemiluminescent detection (ECL). For other non-ECL WB methods, a dilution of 1:1000 to 1:5000 is recommended. A dilution of 1:500 is recommended for ECL WB on mammalian fibrillarin. A dilution of 1:1000 to 1:5000 is recommended for IC on yeast cells and 1:500 for mammalian cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Paeschke K. et al (2005) Telomere end-binding proteins control the formation of G-quadruplex DNA structures in vivo. Nat Struct Mol Biol. 2005 Oct;12(10):847-54. Postberg J. et al (2005) Synthesis of pre-rRNA and mRNA is directed to a chromatin-poor compartment in the macronucleus of the spirotrichous ciliate Stylonychia lemnae. Chromosome Res. 2006;14(2):161-75. Du T.G. et al (2008) Nuclear transit of the RNA-binding protein She2 is required for translational control of localized ASH1 mRNA. EMBO Rep. 2008 Aug;9(8):781-7. Srivastava L. et al (2010) Mammalian DEAD box protein Ddx51 acts in 3' end maturation of 28S rRNA by promoting the release of U8 snoRNA. Mol Cell Biol. 2010 Jun;30(12):2947-56. Sasano Y. et al (2008) Distribution of U3 small nucleolar RNA and fibrillarin during early embryogenesis in Caenorhabditis elegans. Biochimie. 2008 Jun;90(6):898-907. Meng L. et al (2007) Nucleolar trafficking of nucleostemin family proteins: common versus protein-specific mechanisms. Mol Cell Biol. 2007 Dec;27(24):8670-82.
Specificity:
The specificity of this antibody has been confirmed by WB. This clone was selected because it is specific for the ~34 kDa Fibrillarin. It stains a single band on western blotting and shows a clear and strong punctate staining of yeast nuclei. It can therefore be used as a marker for nucleoli in a wide variety of species. Human, Rat, Drosophila, S. pombe, C. elegans and and S. pombe. Predicted to react with other mammalian tissues.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Clone OX-42 recognises the rat equivalent of human CD11b and shares a common epitope with CB11c (integrin apha M and alpha X chains). (PMID:1672643; Tamatani T et al 1991). CD11b is a single-pass type I membrane protein that belongs to the integrin alpha chain family. CD11b is predominantly expressed in monocytes and granulocytes and is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles (Ref: SWISSPROT). CD11b is also frequently used as a microglial marker allowing to distinguish between quiescent and activated microglia based on the intensity of CD11b staining. Moreover the OX-42 monoclonal antibody specifically binds to the CR3 complement (C3bi) receptor found on most monocytes, granulocytes, macrophages, dendritic cells, and microglia. OX-42 antibody inhibits C3bi binding activity.<br />CD11b, also known as integrin alpha M or Mac-1, and is a component of complement receptor 3 (CR3). CD11c, also known as integrin alpha X, and is a component of complement receptor 4 (CR4). Integrin alpha-X/beta-2 is a receptor for fibrinogen. CD11b and CD11c are expressed on immune cells such as macrophages, monocytes, granulocytes, and dendritic cells. OX42 has also been shown to detect microglia in the brain, as well as cells of the liver and epidermis.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS containing no preservatives.
Host Animal:
Mouse
Species Reactivity:
Rat
Immunogen:
Rat peritoneal macrophages, whole cells. (Robinson, AP et al Immunology 1986 57 239-247)
Applications:
ICC,IHC-Paraffin-embedded
Clone number:
OX42, OX-42
Antibody Isotype:
IgG2a, kappa
Application Details:
FC: Flow Cytometry: Unfixed cells preferred, acetone fixed or quickly fixed 1% PLP fixed cells can be used. <br>IH: Immunohistochemical studies of rat fresh frozen tissue sections and paraffin-embedded tissue sections following either periodate-lysine-paraformaldehyde (PLP) fixation, or acetone. Works on very lightly PFA fixed, frozen tissues. (perfusion only 4% PFA 10-15' no post-fix). Epitope can be sensitive to fixation. Dilutions detection method dependent 1:100 to 1:200 recommended. <br>IC: Unfixed preferred, or acetone fixed cells; 5-10', 2% PLP fixed cells, 1-2µg/mL. Dilution is detection method dependent. <br>Immunoprecipitation: use rabbit anti-mouse or anti-mouse IgG beads for capture only. The use of protein A or protein G is not recommended. 1-5µg/mL in restricted volumes. <br>Clone does not work in traditional reduced westerns. Use immunoprecipitation to resolve reactive protein bands.<br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CD11b; CD11B; CD11 antigen-like family member B; ITGAM; Integrin beta 2 alpha subunit;<br>CD11c;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Rana I. et al (2010) Microglia activation in the hypothalamic PVN following myocardial infarction Brain Res. Apr 22;1326:96-104.
Specificity:
Clone OX-42 recognises the rat equivalent of human CD11b and shares a common epitope with CB11c (integrin apha M and alpha X chains). (PMID:1672643; Tamatani T et al 1991). Immunoprecipitates three polypeptides of 160 kDa, 103 kDa and 95 kDa and a fainter band may also be seen at 133 kDa under non-reducing conditions. If the immunoprecipitated proteins are reduced, two major peptides of 163 kDa and 100 kDa and a minor 135 kDa peptide are seen. Mis-information exists concerning reactivity to mouse and human CD11b/c with OX-42 from various vendors. Biosensis has not verified that OX42 reacts with mouse and human, and ONLY recommends the clone only for rat as the original paper and most papers use the OX family of clones on rat.
Storage:
12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Mouse anti-Yellow fluorescent protein (YFP) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC, ELISA.
Background Info:
A monoclonal made against GFP that cross reacts with yellow variants as well as other colored mutants of the protein
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid. PBS, pH 7.4 with 0.02% sodium azide
Host Animal:
Mouse
Species Reactivity:
Species Independent
Immunogen:
Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria N-Terminal peptide-KLH conjugates.
Applications:
ELISA,ICC,IHC-Frozen,WB
Clone number:
1218
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB), ELISA, Dot Blot, Immunoprecipitation, Immunostaining. Western Blotting suggested dilution at 1:1000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
YFP;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Recognizes native and denatured forms of Green Fluorescent Protein (GFP) and its variants: Enhanced Green Fluorescent Protein (EGFP), Yellow Fluorescent Protein (YFP), Enhanced Yellow Fluorescent Protein (EYFP) and Cyan Fluorescent Protein (CFP).
Storage:
Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Mouse anti-Simian Virus Type 5 tag (SV5) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA.
Background Info:
The V5 epitope corresponds to a region from Simian Virus Type 5 (SV5).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid. PBS, pH 7.4 with 0.05% sodium azide
Host Animal:
Mouse
Species Reactivity:
Species Independent
Immunogen:
Synthetic peptide GKPIPNPLLGLDST
Applications:
ELISA,IHC-Frozen,WB
Clone number:
V5.E10
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB), Immunohistochemistry (IHC) and ELISA. Suggested dilutions for WB at 1:500 - 1:1000, ELISA at 1:5,000-10,000 and IHC at 1:50-1:100. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Antibody detects V5-tagged fusion protein.
Storage:
Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Purification:
Antibody was affinity purified from culture supernatant of hybridoma cells grown in a bioreactor. Protein A affinity chromatography.
Mouse anti-Red fluorescent protein (DsRed) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
A monoclonal made againsts recombinant RFP and designed to react specifically against it and its many variants
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid. PBS, pH 7.4 with 0.05% sodium azide
Host Animal:
Mouse
Species Reactivity:
Species Independent
Immunogen:
Recombinant Red Fluorescent Protein (dsRed) expressed from bacteria.
Applications:
ICC,IHC-Frozen,WB
Clone number:
RF5R
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB), Immunohistochemistry (IHC) and Immunoprecipitation (IP). Suggested starting dilutions are as follows: WB at 1:1,000, IP at 5 ug/mg lysate and IHC at 1:200. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
RFP;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Designed to detect Red Fluorescent Protein (RFP) and its variants in ELISA (sandwich or capture), immunoblotting, immunoprecipitation and immunohistochemistry.
Storage:
Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Mouse anti-Myc proto-oncogene protein (Myc) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
The Myc tag contains the amino acids Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu (E-Q-K-L-I-S-E-E-D-L) corresponding to amino acids 410-419 of human Myc. This tag is widely used for monitoring expression of recombinant proteins in bacteria, insect and mammalian cells.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid. PBS, pH 7.4 with 0.05% sodium azide. See vial label for concentration.
Host Animal:
Mouse
Species Reactivity:
Species Independent
Immunogen:
A synthetic peptide (EQKLISEEDL) coupled to KLH.
Applications:
ICC,WB
Clone number:
Myc.A7
Antibody Isotype:
IgG1
Application Details:
Western Blotting (WB), Immunostaining (IS), Immunoprecipitation (IP). Suggested starting dilutions as follows: WB at 1:1,000, IS and IP at 1:200. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Antibody detects over-expressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
Storage:
Stable for 1 year at -20°C from the date of receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Purification:
Protein A affinity chromatography from mouse ascites fluid
Mouse anti-Histidine-tag (His) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
The 6X His tag is a short peptide sequence of 6 histidine residues. Epitopes such as the 6X His tag are often included with the target DNA at the time of cloning to produce fusion proteins containing the tag sequence. This allows anti-epitope tag antibodies such as this one to serve as a universal detection reagent for any recombinant protein containing this tag. Anti-epitope antibodies are a useful alternative to generating antibodies to identify a specific recombinant protein. The 6X His motif is often used as a tag on recombinant proteins to facilitate purification with immobilized metal-affinity chromatography.
Western Blotting (WB), Immunocytochemistry (ICC) and Immunoprecipitation (IP). Suggested starting dilutions are as follows: WB at dilutions of 1:1000, IC and IP at dilutions of 1:200. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Dittmann K. et al (2010) 'Nuclear EGFR shuttling induced by ionizing radiation is regulated by phosphorylation at residue Thr654' FEBS Lett. 2010 Sep 24;584(18):3878-3884. Kolev M.V. et al (2010) 'Upregulating CD59: a new strategy for protection of neurons from complement-mediated degeneration Pharmacogenomics J. 2010 Feb;10(1):12-9.
Specificity:
His-tagged fusion proteins
Storage:
Stable for 1 year at -20°C unopened. After opening we recommend aliquoting and storing at -20°C for up to 6 months. Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap.
The Human influenza hemagglutin (HA) tag corresponds to a region (98-106 amino acids) from the HA molecule.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid. PBS, pH 7.4 with 0.05% sodium azide
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
A synthetic peptide from influenza hemagglutinin epitope (YPYDVPDYA) coupled to KLH.
Applications:
ICC,IHC-Frozen,WB
Clone number:
HA.C5
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB), Immunoprecipitation (IP) and Immunostaining (IS). Suggested dilutions for WB of 1:1000, IP and IS at 1:200. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Antibody detects HA-tagged proteins (on amino- and carboxy termini) in transfected mammalian cells.
Storage:
Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Mouse anti-Glutathione S-transferase protein (GST) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
GST (Glutathione S-Transferase) is a 26 kDa protein encoded by the Schistosoma japonicum.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid. PBS, pH 7.4 with 0.05% sodium azide
Host Animal:
Mouse
Species Reactivity:
Schistosoma Japonicum
Immunogen:
Mouse monoclonal anti-GST tag antibody was produced by immunizing mice with glutathione S-transferase (GST) peptide.
Applications:
ICC,IHC-Frozen,WB
Clone number:
GST.B6
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB), Immunostaining (IS) and Immunoprecipitation (IP). Suggested dilutions for WB of 1:1000, IS and IP at 1:200. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Mouse monoclonal anti-GST tag antibody detects over expressed glutathione- transferase (GST) fusion proteins.
Storage:
Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Mouse anti-D tag Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
The D-tag system utilises a short hydrophilic peptide (DYKDDDDK) that is fused to either the N- or C-terminus of the protein of interest. It can be used in conjunction with other tags such as the 6X His tag.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid. PBS, pH 7.4 with 0.05% sodium azide. See vial label for concentration.
Host Animal:
Mouse
Species Reactivity:
Species Independent
Immunogen:
A synthetic peptide (DYKDDDDK) coupled to KLH.
Applications:
ICC,IHC-Frozen,WB
Clone number:
1000000
Antibody Isotype:
IgG2b, lambda
Application Details:
Western Blotting (WB), Immunostaining (IS) and Immunoprecipitation (IP). Suggested dilutions: WB at 1:100-1:1,000, IS and IP at 1:100-1:1,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Mouse monoclonal anti D-tag antibody recognises over-expressed proteins containing the D-tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
Storage:
Stable for 1 year at -20°C from the date of receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Mouse anti-Cyan fluorescent protein (CFP) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC, ELISA.
Background Info:
A monoclonal made against GFP that cross reacts with the cyan mutants. It is not specific to Cyan isoforms
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid. PBS, pH 7.4 with 0.02% sodium azide
Host Animal:
Mouse
Species Reactivity:
Species Independent
Immunogen:
Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria N-Terminal peptide-KLH conjugates.
Applications:
ELISA,ICC,IHC-Frozen,WB
Clone number:
1218
Antibody Isotype:
IgG
Application Details:
Western Blotting, ELISA, Dot Blot, IPP, Immunostaining. Suggested dilution of 1:1000 for Western Blotting. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CFP;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Recognizes native and denatured forms of Cyan Fluorescent Protein (CFP) and its variants: Enhanced Green Fluorescent Protein (EGFP), Yellow Fluorescent Protein (YFP), Enhanced Yellow Fluorescent Protein (EYFP) and Cyan Fluorescent Protein (CFP).
Storage:
Stable for 1 year at -20°C from the date of shipment (unopened). For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot and maintain at -20°C after opening for up to 6 months. Avoid repeated freezing and thawing.
Mouse anti-Non-specific control IgG Monoclonal Antibody (Unconjugated), suitable for WB, IHC, ICC, FC, ELISA.
Background Info:
Commonly used mouse IgG1 negative control antibody clone derived from a Balb/c myeloma and is recommend as a negative control for a variety of immunohistochemical applications where mouse IgG experimental antibodies are use. To date no published reactivity as been associated with X63.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Host Animal:
Mouse
Species Reactivity:
Non-Reactive (Negative Control)
Immunogen:
None. This control IgG has no known binding ability.
Applications:
ELISA,FC,ICC,IHC,WB
Clone number:
X63
Antibody Isotype:
IgG1, kappa
Application Details:
Recommended for use as a control for Western Blotting, immunohistochemistry and FACS at a concentration equal to that of the test antibody. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
No staining has ever been identified with this immunoglobulin demonstrating its non-specific value as a control. N/A
Storage:
Store lyophilized antibody at 2-8°C. Keep reconstituted antibody at -20°C to -80°C for long-term storage. For short term keep at 2-8°C. We suggest that the customer aliquots the antibody into smaller lots to avoid repeated freezing and thawing.
Purification:
Immunoglobulin purified using Protein G column. Purity analysed using gel electrophoresis.
Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) due to its molecular mass and its ability to bind at low affinity not only NGF (see 162030), but also other neurotrophins, including brain-derived neurotrophic factor (BDNF; 113505), neurotrophin-3 (NTF3; 162660), and neurotrophin-4/5 (NTF5; 162662). At the time of its discovery, NGFR was considered a unique type of protein. Subsequently, however, a large superfamily of tumor necrosis factor receptors were found to share the overall structure of NGFR (4 extracellular ligand-binding, cysteine-rich repeats, or CRs, and signaling through association with, or disassociation from, cytoplasmic interactors). The identification of this superfamily helped elucidate some of the biologic functions of NGFR, including its ultimate involvement in the nuclear factor kappa-B (NFKB; see 164011) and apoptosis pathways. As a monomer, NGFR binds NGF with low affinity. Higher affinity binding is achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA (NTRK1; 191315), TRKB (NTRK2; 600456), and TRKC (NTRK3; 191316). TRKA, TRKB, and TRKC are specific for or 'preferred by' NGF, NTF5 and BDNF, and NTF3, respectively (Ip et al., 1993). NTF3 also binds to TRKA and TRKB, but with significantly lower affinity
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid (1 mg/mL) in PBS, pH 7.2-7.6 without preservatives. Typical Fluorophore/Protein (F/P) - ratio is 3-10.
Host Animal:
Mouse
Species Reactivity:
Cat,Dog,Human,Pig,Rabbit,Sheep
Immunogen:
The p75NTR antibody was derived from immunization of mice with human WM245 melanoma cells.
Applications:
ICC
Clone number:
ME20.4
Antibody Isotype:
IgG1, kappa
Application Details:
This antibody is recommended for use in immunohistochemistry, immunofluorescence, flow cytometry and NGF receptor p75 dynamics. For immunohistochemistry a concentration of 2 µg/mL is recommended. Not appropriate for Western Blots. For FACS a concentration of 20 µg/mL is recommended and for 1 site ELISA at least a 1 in 5000 dilution. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
This antibody recognises p75NTR (low affinity neurotrophin receptor) Reacts with human, cat, dog, pig, rabbit and sheep. Does not react with rat or mouse.
Storage:
Aliquot antibody and store frozen at -20°C to -80°C. For short-term storage, the antibody conjugate can be stored at 2-8°C for up to 4 months with the addition of appropriate antibacterial agent, or for up to 1 week without the addition of a preservative.
Purification:
Protein G purified IgG was labelled with ATTO 488 and free dye removed by gel filtration.
Monoclonal antibody MC192 against the rat low affinity nerve growth factor receptor (p75NTR) is derived from the fusion of Sp2/0-Ag 14 myeloma cells with mouse immune splenocytes. MC192 monoclonal antibody was originally generated by Chandlers et al. p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. MC192 has a potential use as the ligand for gene delivery into p75NTR-expressing rat cells via a receptor-mediated mechanism. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1 (By similarity).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Host Animal:
Mouse
Species Reactivity:
Rat
Immunogen:
Rat p75NTR
Applications:
FC,ICC,IHC-Frozen,IHC-Paraffin-embedded
Clone number:
MC192
Antibody Isotype:
IgG1
Application Details:
IF: live or lightly fixed cells or tissues (acetone or 4% PFA): 2-5µg/mL. Not suitable for western blots; not suitable for IH on formalin fixed tissues. FACS (20µg/mL) is recommended, unfixed cells.
Davies A. et al (2010) The alpha2delta subunits of voltage-gated calcium channels form GPI-anchored proteins, a post translational modification essential for function Proc Natl Acad Sci U S A. Jan 26;107(4):1654-9
Specificity:
MC192 recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat and does not react with human or mouse NGFR. Reacts with rat. Does not react with mouse or human NGFR
Storage:
The antibody conjugate can be stored at 2-8°C for up to 4 months with the addition of appropriate antibacterial agent.
Purification:
Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The IgG was then conjugated to ATTO 488 (ATTO TEC) and purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
Mouse anti-p75 neurotrophin receptor (p75NTR) Monoclonal Antibody (FITC), suitable for IHC-Frozen, ICC, FC.
Background Info:
Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) due to its molecular mass and its ability to bind at low affinity not only NGF (see 162030), but also other neurotrophins, including brain-derived neurotrophic factor (BDNF; 113505), neurotrophin-3 (NTF3; 162660), and neurotrophin-4/5 (NTF5; 162662). At the time of its discovery, NGFR was considered a unique type of protein. Subsequently, however, a large superfamily of tumor necrosis factor receptors were found to share the overall structure of NGFR (4 extracellular ligand-binding, cysteine-rich repeats, or CRs, and signaling through association with, or disassociation from, cytoplasmic interactors). The identification of this superfamily helped elucidate some of the biologic functions of NGFR, including its ultimate involvement in the nuclear factor kappa-B (NFKB; see 164011) and apoptosis pathways. As a monomer, NGFR binds NGF with low affinity. Higher affinity binding is achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA (NTRK1; 191315), TRKB (NTRK2; 600456), and TRKC (NTRK3; 191316). TRKA, TRKB, and TRKC are specific for or 'preferred by' NGF, NTF5 and BDNF, and NTF3, respectively. NTF3 also binds to TRKA and TRKB, but with significantly lower affinity.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid. 10mM Tris, 50mM NaCl
Host Animal:
Mouse
Species Reactivity:
Cat,Dog,Human,Pig,Rabbit,Sheep
Immunogen:
The p75NTR antibody was derived from immunization of mice with human WM245 melanoma cells.
Applications:
FC,ICC,IHC-Frozen
Clone number:
ME20.4
Antibody Isotype:
IgG1
Application Details:
This antibody is recommended for use in immunohistochemistry, immunofluorescence, flow cytometry and NGF receptor p75 dynamics. For immunohistochemistry a concentration of 2 µg/mL is recommended. Not appropriate for Western Blots. For FACS a concentration of 20 µg/mL is recommended and for 1 site ELISA at least a 1 in 5000 dilution. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
This antibody recognises p75NTR (low affinity neurotrophin receptor) Reacts with human, cat, dog, pig, rabbit and sheep. Does not react with rat or mouse.
Storage:
The antibody conjugate can be stored at 2-8°C for up to 4 months with the addition of appropriate antibacterial agent.
Purification:
Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The antibody was then conjugated to Fluorescein isomer 1 (FITC, Sigma). A minimum fluorescein: protein ratio of 3:1 is guaranteed. The conjugate was purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
Mouse anti-p75 neurotrophin receptor (p75NTR) Monoclonal Antibody (FITC), suitable for IHC-Frozen, ICC, FC.
Background Info:
Monoclonal antibody MC192 against the rat low affinity nerve growth factor receptor (p75NTR) is derived from the fusion of Sp2/0-Ag 14 myeloma cells with mouse immune splenocytes. MC192 monoclonal antibody was originally generated by Chandlers et al. p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. MC192 has a potential use as the ligand for gene delivery into p75NTR-expressing rat cells via a receptor-mediated mechanism. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Host Animal:
Mouse
Species Reactivity:
Rat
Immunogen:
Rat p75NTR
Applications:
FC,ICC,IHC-Frozen
Clone number:
MC192
Antibody Isotype:
IgG1
Application Details:
Immunohistochemistry, immunofluorescence, flow cytometry, NGF receptor p75 dynamics, retrograde transport studies, study of intracellular trafficking. Suggested working dilutions: For immunohistochemistry a concentration of 1-2 µg/mL is recommended. The antibody is not appropriate for Western Blots. The recommended concentration for FACS is 20 µg/mL and at least 1 in 5000 dilution is recommended for 1-site ELISA. Optimal working dilution should be determined by the end user. MC192 is not suitable as a blocking agent, although it has been incorrectly used for this purpose in many published works. The antibody was generated specifically by screening for monoclonals that had the ability to ENHANCE the binding of NGF, the natural ligand for p75. Therefore, this antibody is particularly unusual. The full details can be found in the original paper, which is listed on our datasheet (see Chandler et al, 1984). Biosensis recommends optimal dilutions/concentrations should be determined by the end user. The FITC version of MC192 is primarily targeted for FACS or IF applications on live or lightly fixed cells. Antibody will not work in traditional formalin fixed tissues.
Davies A. et al (2010) The alpha2delta subunits of voltage-gated calcium channels form GPI-anchored proteins, a post translational modification essential for function Proc Natl Acad Sci U S A. Jan 26;107(4):1654-9
Specificity:
MC192 recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat. Reacts with rat. Does not react with mouse or human p75 NGFR
Storage:
The antibody conjugate can be stored at 2-8°C for up to 4 months with the addition of appropriate antibacterial agent.
Purification:
Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The antibody was then conjugated to Fluorescein isomer 1 (FITC, Sigma). A minimum fluorescein: protein ratio of 3:1 is guaranteed. The conjugate was purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
Mouse anti-p75 neurotrophin receptor (p75NTR) Monoclonal Antibody (Unconjugated), suitable for IHC-Frozen, FC.
Background Info:
Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) due to its molecular mass and its ability to bind at low affinity not only NGF (see 162030), but also other neurotrophins, including brain-derived neurotrophic factor (BDNF; 113505), neurotrophin-3 (NTF3; 162660), and neurotrophin-4/5 (NTF5; 162662). At the time of its discovery, NGFR was considered a unique type of protein. Subsequently, however, a large superfamily of tumor necrosis factor receptors were found to share the overall structure of NGFR (4 extracellular ligand-binding, cysteine-rich repeats, or CRs, and signaling through association with, or disassociation from, cytoplasmic interactors). The identification of this superfamily helped elucidate some of the biologic functions of NGFR, including its ultimate involvement in the nuclear factor kappa-B (NFKB; see 164011) and apoptosis pathways. As a monomer, NGFR binds NGF with low affinity. Higher affinity binding is achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA (NTRK1; 191315), TRKB (NTRK2; 600456), and TRKC (NTRK3; 191316). TRKA, TRKB, and TRKC are specific for or 'preferred by' NGF, NTF5 and BDNF, and NTF3, respectively (Ip et al., 1993). NTF3 also binds to TRKA and TRKB, but with significantly lower affinity
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Host Animal:
Mouse
Species Reactivity:
Cat,Dog,Human,Pig,Rabbit,Sheep
Immunogen:
The p75NTR antibody was derived from immunization of mice with human WM245 melanoma cells.
Applications:
FC,IHC-Frozen
Clone number:
ME20.4
Antibody Isotype:
IgG1
Application Details:
Immunohistochemistry, immunofluorescence, flow cytometry. Suggested working dilutions: For Immunohistochemistry a concentration of 2 µg/mL is recommended. Antibody not appropriate for Western Blot. For FACS a concentration of 20 µg/mL is recommended. At least 1 in 5000 dilution is recommended for 1 site ELISAs. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Liu W. et al (2012) Distribution of P75 neurotrophin receptor in adult human cochlea-an immunohistochemical study. Cell Tissue Res. 2012 Mar 31. Inoue K. et al. (2009) Differential expression of stem-cell-associated markers in human hair follicle epithelial cells. Lab Invest. 2009 Aug;89(8):844-56. Ariga M. et al. (2008) Functional role of sortilin in myogenesis and development of insulin-responsive glucose transport system in C2C12 myocytes J Biol Chem. 2008 Apr 11;283(15):10208-20 Rogers ML et al (2010) ProNGF mediates death of Natural Killer cells through activation of the p75NTR-sortilin complex. J Neuroimmunol. 2010 Sep 14;226(1-2):93-103. Jiao et al. Differentiation defect in neural crest-derived smooth muscle cells in patients with aortopathy associated with bicuspid aortic valves. EBioMedicine (2016) 10:282-90.
Specificity:
This antibody recognises p75NTR (low affinity neurotrophin receptor) Reacts with human, cat, dog, pig, rabbit and sheep. Does not react with rat or mouse.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Purification:
Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS and then lyophilized. Purity was analysed using electrophoresis, 4-12% Bis Tris Gel (Invitrogen).
Monoclonal antibody MC192 against the rat low affinity nerve growth factor receptor (p75NTR) is derived from the fusion of Sp2/0-Ag 14 myeloma cells with mouse immune splenocytes. MC192 monoclonal antibody was originally generated by Chandlers et al. p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. MC192 recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat. MC192 antibody may be used for immunocytochemical localisation of rat cells expressing p75NTR, ELISA and western blot. This antibody has also been used for the construction of the MC192-saporin immunotoxin for specific elimination of neuronal populations in basal forebrain cholinergic neurons to generate an animal model for Alzheimer's disease. Using Flow Cytometry, this antibody has frequently been employed for panning to isolate p75NTR-expressing rat cells. MC192 has a potential use as the ligand for gene delivery into p75NTR-expressing rat cells via a receptor-mediated mechanism. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1 (By similarity). Interacts with BEX1. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. DOMAIN: Death domain is responsible for interaction with RANBP9. PTM: N- and O-glycosylated. PTM: Phosphorylated on serine residues. SIMILARITY: Contains 1 death domain. SIMILARITY: Contains 4 TNFR-Cys repeats.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Host Animal:
Mouse
Species Reactivity:
Rat
Immunogen:
NGF receptor
Applications:
ELISA,IHC-Frozen,WB
Clone number:
MC192
Antibody Isotype:
IgG1
Application Details:
IH (lightly fixed), ELISA, WB, Flow Cytometry (2 ug per 10^6 cells) IP (non-reducing conditions only!; do not use reducing agents such as DTT or beta-mercaptoethanol), Traditional formalin fixed paraffin embedded immunohistochemistry is NOT recommended with MC192. Motor neuron isolation, Gene/Toxin Delivery to rat sensory/motor neurons. A working solution of 1-2 µg/mL was determined by immunohistochemical staining on 4% paraformaldehyde fixed, or alcohol fixed rat spinal cord and brain. For non-denatured WB, 1-5 µg/mL was found to be suitable with suitable controls (PC12 lysate). ELISA: detection only, 1-5 µg/mL has been suggested in literature.Immunoprecipitation: 5 µg/mL, > 0.5% triton X-100 buffer/500 ug/lysate; PC12 positive control strong suggested. MC192 is not suitable as a blocking agent, although it has been incorrectly used for this purpose in many published works. The antibody was generated specifically by screening for monoclonals that had the ability to ENHANCE the binding of NGF, the natural ligand for p75. Therefore, this antibody is particularly unusual. The full details can be found in the original paper, which is listed on our datasheet (see Chandler et al, 1984). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Riffault B, Kourdougli N, Dumon C, Ferrand N, Buhler E, Schaller F, Chambon C, Rivera C, Gaiarsa JL, Porcher C (2016) Pro-Brain-Derived Neurotrophic Factor (proBDNF)-Mediated p75NTR Activation Promotes Depolarizing Actions of GABA and Increases Susceptibility to Epileptic Seizures. Cereb. Cortex [Epub ahead of print]. Application: Western Blot ; Species: Rat Brandli A, Johnstone DM, Stone J (2016) Remote Ischemic Preconditioning Protects Retinal Photoreceptors: Evidence From a Rat Model of Light-Induced Photoreceptor Degeneration. Invest Ophthalmol Vis Sci. 57(13):5302-13 Application: Western Blot, IHC ; Species: Rat Riffault B, Medina I, Dumon C, Thalman C, Ferrand N, Friedel P, Gaiarsa JL, Porcher C. (2014) "Pro-Brain-Derived Neurotrophic Factor Inhibits GABAergic Neurotransmission by Activating Endocytosis and Repression of GABAA Receptors." J. Neurosci. 34(40):13516-34 Application: Western Blot ,Neuronal cells and hippocampi; Species: Rat Kalincik T et al (2011) Selected changes in spinal cord morphology after T4 transection and olfactory ensheathing cell transplantation. Auton Neurosci. 158(1-2):31-8 Application: IF ; Species: Rat Wu A et al (2011) Delayed olfactory ensheathing cell transplants reduce nociception after dorsal root injury. Exp Neurol. 229(1):143-57 Application: IF ; Species: Rat Davies A et al (2010) The alpha2delta subunits of voltage-gated calcium channels form GPI-anchored proteins, a post translational modification essential for function Proc Natl Acad Sci U S A. Jan 26;107(4):1654-9 Kalincik T et al (2010) Olfactory ensheathing cells reduce duration of autonomic dysreflexia in rats with high spinal cord injury. Auton Neurosci. 154 (1-2):20-9 Application: IHC ; Species: Rat Wilson-Gerwing T.D. et al (2009) J Comp Neurol. 2009 Sep 1;516(1):49-58 Feron F et al (2008) Neurotrophin expression in the adult olfactory epithelium. Brain Res. 1196:13-21 Application: IHC ; Species: Rat Bianco JI et al (2004) Neurotrophin 3 promotes purification and proliferation of olfactory ensheathing cells from human nose. Glia. 45(2):111-23 Application: IHC, IF ; Species: Rat Eyles D et al (2003) Neuroscience. 2003;118(3):641-53. Application: IHC ; Species: Rat Lu J et al (2001) Transplantation of nasal olfactory tissue promotes partial recovery in paraplegic adult rats. Brain Res. 889(1-2):344-57 Application: IF ; Species: Rat
Specificity:
MC192 is specific only for RAT NGFR, no reactivity to Human or Mouse NGFR has been reported This monoclonal antibody has been tested for immunohistochemical localisation of p75NTR-expressing rat cells in the spinal cord and brain. This monoclonal antibody does not cross react with p75NTR-expressing cells in other species.
Storage:
The MC192 is supplied in lyophilized form from Protein G-purified hybridoma cell culture supernatants. The lyophilized antibody is stable when stored at 2-8°C or -20°C. After reconstitution undiluted aliquots should be kept at -20°C for up to six months. For additional stability Glycerol (1:1) may be added after reconstitution. Repetitive freeze/thaw cycle should be avoided.
Neurotrophins are growth factors that regulate neuronal development, maintenance and plasticity. These homo-dimeric proteins activate a multitude of intracellular signalling cascades through their interaction with Trk receptors and p75NTR. The Neurotrophin Antibody Kit (Trial Size) contains a collection of Biosensis' popular sheep antibodies raised against BDNF ( S-015-500 ), NGF ( S-052-500 ), NT3 ( S-057-500 ) and NT4/5 ( S-059-500 ). The kit is completed with one vial of normal sheep IgG ( S-1754-100 ) for use as negative control. This kit presents a cost-effective way to investigate neurotrophin protein expression and function by immunological techniques such as immunohistochemistry, ELISA, and inhibition of biological activity.
Background Info:
Neurotrophins are growth factors that regulate neuronal development, maintenance and plasticity. These homo-dimeric proteins activate a multitude of intracellular signalling cascades through their interaction with Trk receptors and p75NTR. <br /><br />The Neurotrophin Antibody Kit (Trial Size) contains a collection of Biosensis' popular sheep antibodies raised against BDNF (<a href="https://www.biosensis.com/sheep-antibody-bdnf-p-61.htmL" target="_blank" class="newA">S-015-500</a>), NGF (<a href="https://www.biosensis.com/sheep-antibody-beta-p-164.htmL" target="_blank" class="newA">S-052-500</a>), NT3 (<a href="https://www.biosensis.com/sheep-antibody-p-172.htmL" target="_blank" class="newA">S-057-500</a>) and NT4/5 (<a href="https://www.biosensis.com/sheep-antibody-p-176.htmL" target="_blank" class="newA">S-059-500</a>). The kit is completed with one vial of normal sheep IgG (<a href="https://www.biosensis.com/normal-sheep-from-immunized-animal-protein-purified-p-1585.htmL" target="_blank" class="newA">S-1754-100</a>) for use as negative control. This full size version of Biosensis' Neurotrophin Antibody Kit contains 500 ?g sheep IgG of each antibody.<br /><br />This kit presents a cost-effective way to investigate neurotrophin protein expression and function by immunological techniques such as immunohistochemistry, ELISA, and inhibition of biological activity.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4, without preservatives.
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
BDNF: recombinant human BDNF<br>NGF: native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)<br>NT3: recombinant human NT3<br>NT4/5: recombinant human NT4
Applications:
IHC,Neutralize
Antibody Isotype:
IgG
Application Details:
<b>Immunohistochemistry</b>: 1-10 µg/mL. BDNF antibody works best on Zamboni's fixed, frozen tissue, and is not recommended for paraffin-embedded tissue.<br><b>ELISA:</b> 1-10 µg/mL.<br><b>Western Blotting:</b> 1-10 µg/mL. Western blotting is not recommended for the BDNF antibody.<br><b>Inhibition of biological activity in vitro/in vivo:</b> Expected ED50 (in vivo) values are in the range of 2-10 µg/mL. The NGF antibody completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro.<br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user. The negative control (normal sheep IgG) should be used at the same concentration as the neurotrophin antibodies.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
BDNF Antibody: A cross-reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by ELISA. NGF Antibody: A cross-reactivity of less than 1% against recombinant human BDNF, NT3 and NT4/5 by ELISA has been shown. NT3 Antibody: A cross-reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by ELISA. NT4/5 Antibody: Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by dot blot. Neurotrophins share a large degree of homology among human, rodent and other mammalian species, thus these neurotrophin antibodies are useful for a number of species, including human, mouse and rat.
Storage:
Store lyophilized, unopened vials at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Neurotrophins are growth factors that regulate neuronal development, maintenance and plasticity. These homo-dimeric proteins activate a multitude of intracellular signalling cascades through their interaction with Trk receptors and p75NTR. The Neurotrophin Antibody Kit (Trial Size) contains a collection of Biosensis' popular sheep antibodies raised against BDNF ( S-015-500 ), NGF ( S-052-500 ), NT3 ( S-057-500 ) and NT4/5 ( S-059-500 ). The kit is completed with one vial of normal sheep IgG ( S-1754-100 ) for use as negative control. This kit presents a cost-effective way to investigate neurotrophin protein expression and function by immunological techniques such as immunohistochemistry, ELISA, and inhibition of biological activity.
Background Info:
Neurotrophins are growth factors that regulate neuronal development, maintenance and plasticity. These homo-dimeric proteins activate a multitude of intracellular signalling cascades through their interaction with Trk receptors and p75NTR. <br /><br />The Neurotrophin Antibody Kit (Trial Size) contains a collection of Biosensis' popular sheep antibodies raised against BDNF (<a class="newA" target="_blank" href="https://www.biosensis.com/sheep-antibody-bdnf-p-61.htmL">S-015-500</a>), NGF (<a class="newA" target="_blank" href="https://www.biosensis.com/sheep-antibody-beta-p-164.htmL">S-052-500</a>), NT3 (<a class="newA" target="_blank" href="https://www.biosensis.com/sheep-antibody-p-172.htmL">S-057-500</a>) and NT4/5 (<a class="newA" target="_blank" href="https://www.biosensis.com/sheep-antibody-p-176.htmL">S-059-500</a>). The kit is completed with one vial of normal sheep IgG (<a class="newA" target="_blank" href="https://www.biosensis.com/normal-sheep-from-immunized-animal-protein-purified-p-1585.htmL">S-1754-100</a>) for use as negative control. This trial size of Biosensis' Neurotrophin Antibody Kit contains 100 ?g sheep IgG of each antibody.<br /><br />This kit presents a cost-effective way to investigate neurotrophin protein expression and function by immunological techniques such as immunohistochemistry, ELISA, and inhibition of biological activity.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4, without preservatives.
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
BDNF: recombinant human BDNF<br>NGF: native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)<br>NT3: recombinant human NT3<br>NT4/5: recombinant human NT4
Applications:
IHC,Neutralize
Antibody Isotype:
IgG
Application Details:
<b>Immunohistochemistry</b>: 1-10 µg/mL. BDNF antibody works best on Zamboni's fixed, frozen tissue, and is not recommended for paraffin-embedded tissue.<br><b>ELISA:</b> 1-10 µg/mL.<br><b>Western Blotting:</b> 1-10 µg/mL. Western blotting is not recommended for the BDNF antibody.<br><b>Inhibition of biological activity in vitro/in vivo:</b> Expected ED50 (in vivo) values are in the range of 2-10 µg/mL. The NGF antibody completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro.<br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user. The negative control (normal sheep IgG) should be used at the same concentration as the neurotrophin antibodies.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
BDNF Antibody: A cross-reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by ELISA. NGF Antibody: A cross-reactivity of less than 1% against recombinant human BDNF, NT3 and NT4/5 by ELISA has been shown. NT3 Antibody: A cross-reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by ELISA. NT4/5 Antibody: Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by dot blot. Neurotrophins share a large degree of homology among human, rodent and other mammalian species, thus these neurotrophin antibodies are useful for a number of species, including human, mouse and rat.
Storage:
Store lyophilized, unopened vials at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Guinea Pig anti-Neuropeptide RFRP-3 Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Neuropeptide VF is the precursor of neuropeptides NPSF (RFRP-1), RFRP-2 and RFRP-3 (NPVF). RFRP-3 is reported to inhibit forskolin-induced production of cAMP. RFRP-3 has also been shown to block morphine-induced analgesia.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Guinea Pig
Species Reactivity:
Human,Rat,Sheep
Immunogen:
A synthetic peptide (VPNLPQRF) corresponding to the amino acids 124-131 from human Neuropeptide VF. Neuropeptide VF is the precursor of the neuropeptides NPSF (RFRP-1), RFRP-2 and RFRP-3. The synthetic peptide was conjugated to a carrier protein KLH to enhance the immunological response.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
Immunohistochemistry (IHC). A concentration of 1 in 2000 is recommended. IHC performed in sheep brain (hypothalamus) demonstrates intense staining of cells and terminals. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity was demonstrated by immunohistochemistry. This antibody is known to react with rat and sheep.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Guinea Pig anti-Oxytoxin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. It belongs to the vasopressin/oxytocin family. Oxytocin is secreted.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Guinea Pig
Species Reactivity:
Human,Sheep
Immunogen:
A synthetic peptide (CYIQNCPLG) corresponding to the amino acids 20-28 from human Oxytocin-neurophysin 1. This peptide was conjugated to carrier protein to enhance the immunological response.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A concentration of 1 in 2000 is recommended for IHC. IHC performed in sheep brain (hypothalamus) demonstrates intense staining of cells and terminals. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/mL of oxytocin. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
OT; OXT;OT-NPI; OT-NPI;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity was demonstrated by immunohistochemistry. This antibody is known to react with sheep. Other species have not yet been tested.
Storage:
It is recommended that a thawed sample is stored at 2-8°C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20°C or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Luteinizing hormone promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. It exists as a heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin, and gonatropin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Guinea Pig
Species Reactivity:
Sheep
Immunogen:
A synthetic peptide corresponding to the antigenic region within the ovine LH residue.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A concentration of 1 in 3000 is recommended for IHC. IHC performed in sheep pituitary demonstrates intense staining of cells. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/mL of LH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Sanchez NS, Quinn KE, Ashley AK, Ashley RL (2017). In the ovine pituitary, CXCR4 is localized in gonadotropes and somatotropes and increases with elevated serum progesterone. Domest Anim Endocrinol. In press.
Specificity:
Specificity was demonstrated by immunohistochemistry. This antibody is known to react with sheep. Other species have not yet been tested
Storage:
It is recommended that a thawed sample is stored at 2-8°C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20°C or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Guinea Pig anti-Ghrelin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) and upon binding to the receptor it induces the release of growth hormone from the pituitary. This ligand has an appetite-stimulating effect and is involved in growth regulation.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Guinea Pig
Species Reactivity:
Human,Sheep
Immunogen:
A synthetic peptide (GSSFLSPEHQRVQQRKESKKPPAKLQPR) corresponding to the amino acids 24-51 from human ghrelin. This peptide was conjugated to carrier protein to enhance the immunological response.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A concentration of 1 in 3000 is recommended for IHC. IHC performed in sheep abomasum demonstrates intense staining of cells and terminals. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/mL of human ghrelin. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity was demonstrated by immunohistochemistry. This antibody is known to react with sheep and human. Other species have not yet been tested.
Storage:
It is recommended that a thawed sample is stored at 2-8°C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20°C or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
<div align="justify">Proopiomelanocortin (POMC) is the precursor of the melanocortin peptides alpha, beta and gamma. POMC neurons in the arcuate nucleus of the hypothalamus function as key central regulators of food intake and homeostatic control of energy balance in both rodents and non-human primates.</div>
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Guinea Pig
Species Reactivity:
Human,Sheep
Immunogen:
A synthetic peptide (YVMGHFRWDRFG) corresponding to the amino acids 77-88 from human Proopiomelanocortin (POMC). POMC is the precursor of the melanocortin peptides alpha, beta and gamma. The synthetic peptide was conjugated to carrier protein to enhance the immunological response.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A concentration of 1 in 2000 is recommended for IHC. This antibody shows excellent staining on sheep and human tissue. Rodent tissues demonstrate high background staining. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human. Specificity was demonstrated by immunohistochemistry. This antiserum is known to react with sheep gamma MSH. Other species have not yet been tested.
Storage:
It is recommended that a thawed sample is stored at 2-8°C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20°C or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Proopiomelanocortin (POMC) is the precursor of the melanocortin peptides alpha, beta and gamma. POMC neurons in the arcuate nucleus of the hypothalamus function as key central regulators of food intake and homeostatic control of energy balance in both rodents and non-human primates. MSH increases the pigmentation of skin by increasing the melanin production in melanocytes. MSH is produced in the pituitary gland and belongs to the POMC family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Guinea Pig
Species Reactivity:
Human,Rat,Sheep
Immunogen:
A synthetic peptide (Ac-SYSMEHFRWGKPV) corresponding to the amino acids 138-150 from human Proopiomelanocortin (POMC). POMC is the precursor of the melanocortin peptides alpha, beta and gamma. The synthetic peptide was conjugated to a carrier protein to enhance the immunological response.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A concentration of 1 in 2,000 is recommended for IHC. IHC performed in sheep brain (hypothalamus) demonstrates intense staining of cells. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/mL of alpha MSH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity was demonstrated by immunohistochemistry. This antibody is known to react with human, rat and sheep. Other species have not yet been tested.
Storage:
It is recommended that a thawed sample is stored at 2-8°C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20°C or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Guinea Pig anti-Agouti related protein (AGRP) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
AGRP is the endogenous antagonist of alpha-melanocyte stimulating hormone and has been shown to cause potent stimulation of food intake, and this protein is found in over 90% of Neuropeptide Y containing cells in rats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Guinea Pig
Species Reactivity:
Human,Mouse,Rat,Sheep
Immunogen:
A synthetic peptide (SPRRCVRLHESCLGQQVPCCDPCATCYCRFFNAFCYCRKLGTATNLCSRT) corresponding to a region (82-131) within the carboxy domain of mouse agouti related protein. This peptide was conjugated to carrier protein to enhance the immunological response.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC, frozen, PFA fixed material. Not yet tested on paraffin embedded tissue sections. A concentration of 1:1000 to 1:2000 is recommended for IHC with overnight incubations. Permeabilization suggested is 0.1% triton X-100 in blocking buffer. IHC performed in sheep brain (hypothalamus) demonstrates intense staining of cells and terminals. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/mL of AGRP. In rodents such as rat cell terminal staining is most typically observed without cell body staining. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Agrp; Agrt; Art; Agouti-related protein;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity was demonstrated by immunohistochemistry. This antibody is known to react with human, rat and sheep. Other species have not yet been tested.
Storage:
Store lyophilized antibody at 2-8ºC. It is recommended that a thawed sample is stored at 2-8°C for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20°C or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
Goat anti-Green fluorescent protein (GFP) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
The green fluorescent protein (GFP) is a 27 kDa protein isolated originally from the jellyfish Aequoria victoria. It has an endogenous fluorochrome activity with excitation maximum at 395 nm and emission maximum at 509 nm, which is similar to that of fluorescein. GFP can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell.<br> This GFP rabbit antibody was made against a recombinant GFP construct originating from an Aequoria species which was engineered to improve spectral properties and prevent oligomerization. This form of GFP, referred to as AcGFP, is 94% identical to the eGFP developed by Tsien and co-workers. The antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Goat
Species Reactivity:
Species Independent
Immunogen:
Recombinant AcGFP protein expressed in and purified from E. Coli.
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:2,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Green fluorescent protein, GFP
Biosensis Brand:
Biosensis®
Cellular Localisation:
Intracellular, cytosolic.
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Immunogen length:
Full-length recombinant protein.
Negative Control:
Non-transfected HEK293 cells.
Physical State:
Solid.
Positive Control:
GFP-transfected HEK293 cells.
Specificity:
Specific for GFP, does not cross-react with mCherry.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Product Validation Info:
Antibody recognizes GFP protein in GFP-transfected HEK293 cells, but not in non-transfected control cells.
Purification:
Affinity-purified from goat serum using the immunogen.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Goat
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB and direct ELISA. A dilution range of 1:1000 - 1:5000 is recommended for IHC and WB. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor; Beta-NGF; NGF; NGFB;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF. Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Goat anti-Apolipoprotein E (ApoE) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Apolipoprotein E (ApoE) is a lipoprotein involved in fat metabolism and acts as cholesterol carrier between cells and across tissues. On a genetic level, three APOE alleles are described, APOE2, APOE3 and APOE4. These alleles give rise to six APOE isoforms, which are differentially implicated in various diseases. In the peripheral system, APOE4 is linked to increased risk of atherosclerosis. In the CNS, the ability of APOE4 in clearing beta-amyloid is impaired, while APOE3 and APOE2 are more efficient in performing this task. The APOE4 genotype in particular has been linked to increased risk for developing Alzheimer's Disease.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from a solution containing 50 mM Tris, pH 7.5, 0.4 M NaCl, 0.01 M EDTA, 3% trehalose, 0.07% sodium azide.
Host Animal:
Goat
Species Reactivity:
Human
Immunogen:
Recombinant human Apolipoprotein E
Applications:
ELISA
Antibody Isotype:
IgG
Application Details:
ELISA (0.1-1 µg/mL). Other applications not tested as yet. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
APOE;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human. Species cross-reactivity not tested.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution keep aliquots at -20°C to -80°C for higher stability. Avoid repetitive freeze/thaw cycles.
Goat anti-C-reactive protein (CRP) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. COFACTOR: Binds 2 calcium ions per subunit.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Goat
Species Reactivity:
Human
Immunogen:
Human recombinant C-Reactive protein
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. Extremely high titre antibody. For western blot a dilution of 1: 10,000 to 1: 100000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CRP; PTX1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
It has been shown to be specific for C-reactive protein by WB. Human
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent.
This cell lysate is suitable as positive control for Western Blotting, to confirm TrkB phosphorylation at amino acid S478 (rat/mouse) or S479 (human), respectively, using TrkB (pS478/479) rabbit antibody R-1718-50. It is particular useful for complex Western Blotting samples to identify TrkB (pS478/479) immunoreactive bands. This lysate has been prepared by triggering TrkB phosphorylation in retinoic acid-treated mouse NSC34 cells with mature BDNF, and subsequent processing with RIPA buffer. The cell lysate is provided lyophilised for extended stability.
Background Info:
Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity (By similarity). Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia (Ref: uniprot.org).
Product Type:
Cell Lysate
Format:
Lyophilized from a RIPA cell lysate preparation, without preservatives.
Applications:
WB
Application Details:
Western Blotting (5 - 10 µg loading per lane). Biosensis recommends optimal loading amounts should be determined by the end user.
Store lyophilized lysate at 2-8°C. After reconstitution divide into single-use aliquots and store at -80°C for long-term storage. Aliquots should be used within 1 hour after thawing. Avoid repetitive freeze/thaw cycles.
Product Validation Info:
This product has been validated for TrkB (S478/479) phosphorylation by Western Blotting, using rabbit antibody R-1718-50 to TrkB (pS478/479). Treatment of blotting membrane with lambda-phosphatase obliterates TrkB pS478/479 immunoreactivity.
Purification:
This lysate has been prepared in RIPA buffer as crude cell lysate.
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Chicken
Species Reactivity:
Human, Mouse, Rat
Immunogen:
C-terminal peptide of human IBA1. The antibody has been made against the C-terminal peptide of human IBA1 coupled to keyhole limpet hemocyanin (KLH).
Applications:
ICC, IHC-Frozen, WB
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:100 - 1:500 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Chicken anti-Green fluorescent protein (GFP) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
The green fluorescent protein (GFP) is a 27 kDa protein isolated originally from the jellyfish Aequoria victoria. It has an endogenous fluorochrome activity with excitation maximum at 395 nm and emission maximum at 509 nm, which is similar to that of fluorescein. GFP can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell.<br> This GFP rabbit antibody was made against a recombinant GFP construct originating from an Aequoria species which was engineered to improve spectral properties and prevent oligomerization. This form of GFP, referred to as AcGFP, is 94% identical to the eGFP developed by Tsien and co-workers. The antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Species Independent
Immunogen:
Recombinant AcGFP protein expressed in and purified from E. Coli.
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Green fluorescent protein, GFP
Biosensis Brand:
Biosensis®
Cellular Localisation:
Intracellular, cytosolic.
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Immunogen length:
Full-length recombinant protein.
Negative Control:
Non-transfected HEK293 cells.
Physical State:
Solid.
Positive Control:
GFP-transfected HEK293 cells.
Specificity:
Specific for GFP, does not cross-react with mCherry.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Product Validation Info:
Antibody recognizes GFP protein in GFP-transfected HEK293 cells, but not in non-transfected control cells.
Chicken anti-Parvalbumin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Full-length recombinant human protein
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000-1:5,000). Note that this antibody does not recognize parvalbumin in rat or mouse brain homogenates on western blots. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
PVALB; Parvalbumin;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human, reacts with Human, Rat, Mouse. Antibody is specific for parvalbumin and does not recognize closely related proteins calretinin and calbindin as determined by Western Blotting.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Chicken anti-Nestin Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity). Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Human,Rat
Immunogen:
Part of recombinant human protein (amino acids 315-630).
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:2,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NES;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human,reacts with human and rat. Other species not tested.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Chicken anti-Ki-67 Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Recombinant human Ki-67 protein (mixture of amino acids 1-300 and 1,111-1,490) expressed in and purified from <i>E. coli.</i>
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
Western blotting (1:2,000-1:5,000) and Immunocytochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Antigen Ki67
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human, reacts with human only. Does not react with mouse or rat.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Full-length recombinant human protein
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
Western blotting (1:5,000-1:10,000), Immunocytochemistry (1:5,000-1:10,000) and Immunohistochemistry (1:5,000-1:10,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CNPase; CNP;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human, reacts with human, rat, mouse.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Chicken anti-Calretinin (CR) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Calretinin is a calcium-binding protein which is abundant in auditory neurons. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Bovine,Human,Mouse,Rat
Immunogen:
Full-length recombinant human protein
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CR; 29 kDa calbindin;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human, reacts with human, cow, rat, mouse. Antibody is specific for calretinin and does not recognize closely related proteins parvalbumin and calbindin as determined by Western Blotting.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Chicken anti-Calbindin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Buffers cytosolic calcium. May stimulate a membrane Ca<sup>2+</sup>-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Bovine,Human,Mouse,Rat
Immunogen:
Full-length recombinant human protein
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human, reacts with human, cow, rat, mouse. Antibody is specific for calbindin and does not recognize closely related proteins parvalbumin and calretinin as determined by Western Blotting.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. Ref: uniprot.org.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Bovine,Chicken,Horse,Human,Mouse,Pig,Rat
Immunogen:
Full length human protein with the epitope from amino acids 61-95
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
Western blotting (1:1,000 - 1:2,000), Immunocytochemistry (1:1,000 - 1:2,000) and Immunohistochemistry (1:1,000 1: 2,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP; SNCA; PARK1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human, reacts with human, horse, cow, pig, chicken, rat, mouse.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Bovine milk contains two types of beta-casein protein, A2 or A1. Recent studies have shown that milk containing the A1 beta casein protein can contribute to issues including gastrointestinal discomfort after ingestion. There is some evidence of a link between ingestion of A1 beta casein protein and the development of Type 1 diabetes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative. Refer to the product label for antibody concentration.
Host Animal:
Chicken
Species Reactivity:
Bovine
Immunogen:
A synthetic peptide (PGPIHNSLP, aa: 78-86) conjugated to KLH has been used as immunogen. Bovine A2 beta casein differs from bovine A1 beta casein by one amino acid (P82 -> H82)
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
Western blot and ELISA. Suggested working dilution for western blot is 1:1,000-1:5,000. The amount of milk per lane can be 0.05 µL-0.1 µL for Western blot. Sample Preparation: Milk should be diluted 1:10 in 0.1M NaOH. The reason for diluting (1:10) in 0.1M NaOH is because the milk protein is easier to dissolve in NaOH. The sample is then further diluted with PBS or other buffer and mixed at 1:1 ratio, to prepare for loading. For example, one can take the 1:10 dilution milk (0.5 µL-1 µL) and add into 9.5 µL or 9 µL PBS and mix with 10 µL SDS-PAGE Sample buffer, boil for 5 minutes, quick spin, and load on the gel. Recommended blocking buffer: TBS with 5% BSA. Recommended antibody dilution buffer: TBST containing 3% BSA. Biosensis recommends that optimal working dilutions should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody is specific to A1 beta casein by western blot. No cross-reactivity with A2 beta casein is seen. Species cross-reactivity not tested.
Storage:
Maintain unopened vial at -20°C for up to 12 months after date of receipt. After opening maintain at -20°C in undiluted aliquots for up to 6 months. For short-term storage, keep aliquot at 2-8°C for up to one week. Avoid repeated freeze-thaw cycles.
Bovine milk contains two types of beta-casein protein, A2 or A1. Recent studies have shown that milk containing the A1 beta casein protein can contribute to issues including gastrointestinal discomfort after ingestion. There is some evidence of a link between ingestion of A1 beta casein protein and the development of Type 1 diabetes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative. Refer to the product label for antibody concentration.
Host Animal:
Chicken
Species Reactivity:
Bovine
Immunogen:
A synthetic peptide (PGPIPNSLP, aa: 78-86) conjugated to KLH has been used as immunogen. Bovine A1 beta casein differs from bovine A2 beta casein by one amino acid (H82 -> P82)
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
Western blot and ELISA. Suggested working dilution for western blot is 1:200-1:1,000. The amount of milk per lane can be 0.05 µL-0.1 µL for Western blot. Sample Preparation: Milk should be diluted 1:10 in 0.1M NaOH. The reason for diluting (1:10) in 0.1M NaOH is because the milk protein is easier to dissolve in NaOH. The sample is then further diluted with PBS or other buffer and mixed at 1:1 ratio, to prepare for loading. For example, one can take the 1:10 dilution milk (0.5 µL-1 µL) and add into 9.5 µL or 9 µL PBS and mix with 10 µL SDS-PAGE Sample buffer, boil for 5 minutes, quick spin, and load on the gel. Recommended blocking buffer: TBS with 5% BSA. Recommended antibody dilution buffer: TBST containing 3% BSA. Biosensis recommends that optimal working dilutions should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody is specific to A2 beta casein by western blot. No cross-reactivity with A1 beta casein is seen. Species cross-reactivity not tested.
Storage:
Maintain unopened vial at -20°C for up to 12 months after date of receipt. After opening maintain at -20°C in undiluted aliquots for up to 6 months. For short-term storage, keep aliquot at 2-8°C for up to one week. Avoid repeated freeze-thaw cycles.
Chicken anti-Heat shock protein 27 (HSP-27) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
The heat shock proteins were discovered, as the name suggests, since they are heavily upregulated when cells are stressed by temperatures above the normal physiological range. They are expressed in unstressed cells also and have a normal function as chaperones, helping other proteins to fold correctly, and are required in much greater amounts if the cell or tissue is stressed by heat. The increased levels are generated transcriptionally under the influence of a powerful transcription factor, the heat shock factor 1 (HSF1). The different heat shock proteins were originally named based on their SDS-PAGE mobility, so HSP27 has an apparent molecular weight of 27 kDa. It is an abundant protein even under non-stress conditions and frequently shows up as a major spot on 2 dimensional gels of cells or tissues. It is known to associate with a variety of other proteins such as actin, intermediate filament subunits and ubiquitin and is found both in the cytoplasm and the nucleus of cells. HSP27 can become heavily phosphorylated under the influence of multiple protein kinases particularly as a result of activation of the p38/SAPK pathway. Upregulation of this protein is protective against neurodegenerative diseases at least in certain mouse models (1). Point mutations in the HSP27 gene are associated with two neurological diseases, Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuropathy IIB (2). These diseases are associated with axonal loss apparently following defects in the transport of neurofilaments.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Recombinant full length purified HSP27 from E. coli.
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:2,000 - 1:5,000 is recommended for WB. A dilution of 1:1,000 - 1:2,000 is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a 27 kDa band by Western blot on a crude extract from HeLa cells. It has also been used successfully for immunocytochemistry.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Chicken anti-Lamin A/C Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
The Lamin proteins are members of the intermediate filament protein family but are located inside the nucleus rather than in the cytoplasm (1). The lamins function as skeletal components tightly associated with the inner nuclear membrane. Originally the proteins of the nuclear cytoskeleton were named Lamin A, B and C, from top to bottom as visualized on SDS-PAGE gels. Subsequently it was found that Lamins A and C were coded for by a single gene (2), while the Lamin B band may contain two proteins encoded by two genes now called Lamin B1 and Lamin B2. Lamin A has a mass of about 74 kDa while Lamin C is 65 kDa. The Lamin A protein includes 98 amino acids missing from Lamin C, while Lamin C has a C-terminal 6 amino acid peptide not present in Lamin A. Apart from these regions Lamin A and C are identical so that antibodies raised against either protein are likely to cross react with the other, as is the case with this monoclonal. Lamin polymerization and depolymerization is regulated by phosphorylation by cyclin dependent protein kinase 1 (CDK1), the key component of "maturation promoting factor", the central regulator of cell division. Activity of this kinase increases during cell division and is responsible for the breakdown of the nuclear lamina. Mutations in the LMNA gene are associated with several serious human diseases, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease type 2B1, and Hutchinson-Gilford progeria syndrome. This family of diseases belong to a larger group which are often referred to as Laminopathies, though some laminopathies are associated in defects in Lamin B1, B2 or one or other of the numerous nuclear lamina binding proteins. A truncated version of lamin A, commonly known as progerin, causes Hutchinson-Gilford progeria syndrome, a form of premature aging (3).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Full length recombinant human Lamin C
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
Immunocytochemistry (ICC) and Western Blotting (WB). A dilution of 1:1,000-1:2,000 is recommended for WB. A dilution of 1:500-1:1,000 is recommended for ICC. The optimal dilution should be determined by the end user.
Lamin A and Lamin C. The antibody reacts with a 74 kDa and 65 kDa band by Western blot on HeLa cell extract. It has also been used successfully for immunocytochemistry on HeLa cell cultures.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Chicken anti-Microtubule-associated protein tau (MAPT) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
FUNCTION: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. SUBCELLULAR LOCATION: Cytoplasm; cytosol. Cell membrane. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components. ALTERNATIVE PRODUCTS: 8 named isoforms produced by alternative splicing. Additional isoforms seem to exist. Isoforms differ from each other by the presence or absence of up to 5 of the 15 exons. One of these optional exons contains the additional tau/MAP repeat. TISSUE SPECIFICITY: Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system. DEVELOPMENTAL STAGE: Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain. DOMAIN: The tau/MAP repeat binds to tubulin. In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments and straight filaments, mainly composed of hyperphosphorylated forms of Microtubule-associated protein Tau. Defects in Microtubule-associated protein Tau are a cause of frontotemporal dementia and parkinsonism linked to chromosome 17, as well as a number of other neurodegenerative diseases.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Recombinant full length version of the shortest human tau isoform purified from E. coli.
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The antibody reacts with multiple closely spaced bands covering the region of the blot from 48 kDa to 67 kDa, with an additional band at 100 kDa. It has also been used successfully for immunocytochemistry. Expected to react with horse, cow, pig, chicken, rat and mouse.
Storage:
Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.
Chicken anti-Proto-oncogene tyrosine-protein kinase receptor Ret (RET) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
The RET proto-oncogene is a receptor tyrosine kinase for members of the glial cell line-derived neurotrophic factor family of extracellular signalling molecules
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid: Concentrated ammonium sulphate in PBS pH 7.4
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Recombinant His-tagged extracellular fragment of human RET protein produced using CHO cell line. The extracellular fragment of hRET was expressed and secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
WB, ICC. A dilution of 1:5 000 to 1:10 000 is recommended for Western blot and 1:250 to 1:500 for immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Cadherin family member 12; Ret;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human RET
Storage:
Store at 2-8°C upon receipt; DO NOT FREEZE. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use. See reconstitution instructions for more information.
Purification:
Affinity purified
Target:
Proto-oncogene tyrosine-protein kinase receptor Ret (RET)
Chicken anti-GDNF family receptor alpha-4 (GFR alpha-4) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
GFR_-4 belongs to the GDNF receptor family. It is a glycosyl-phosphatidylinositol (GPI)-linked cell surface receptor for persephin, and mediates activation of the RET tyrosine kinase receptor.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7.4 containing no preservatives
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Recombinant His-tagged human GFRa-4 protein produced using CHO-based cell line. For production of hGFRa-4, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
WB, IHC. A dilution of 1:5 000-1:10 000 is recommended for Western blots and 1:1 000 to 1:3 000 for immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Chicken anti-GDNF family receptor alpha-3 (GFR alpha-3) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
The GFRa-3 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Concentrated ammonium sulphate in PBS pH 7.4 contain no preservatives
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Recombinant His-tagged human GFRa-3 protein produced using CHO-based cell line. For production of hGFRa-3, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
WB, ICC. A dilution of 1:5 000 to 1:10 000 is recommended for Western blot and 1:1500 to1:3000 for immunocytochemistry. Biosensis recommends that optimal dilutions/concentrations should be determined by the end user.
Human GFRa-3. No cross-reactivity with hGFRa-1, hGFRa-2, hGFRa-4.
Storage:
Store at 2-8°C upon receipt; DO NOT FREEZE. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use. See reconstitution instructions for more information.
Chicken anti-GDNF family receptor alpha-2 (GFR alpha-2) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GFR_-2 is a member of the GDNF receptor family. It is a glycosylphosphatidyl-inositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NRTN compared to its other family member, GDNF family receptor alpha 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Concentrated ammonium sulphate in PBS pH 7.4 containing no preservatives.
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Recombinant His-tagged human GFR alpha-2 protein produced using CHO-based cell line. For production of hGFR alpha-2, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
WB, ICC. A dilution of 1:5,000 to 1:10,000 is recommended for Western blot and 1:500 to 1:1,000 for Immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
human GFR alpha-2. No cross reaction with hGFR alpha-1, hGFR alpha-3, hGFR alpha-4
Storage:
Store at 2-8°C upon receipt; DO NOT FREEZE. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use. See reconstitution instructions for more information.
Chicken anti-GDNF family receptor alpha-1 (GFR alpha-1) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The GFR_-1 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor (www.ncbi.nlm.nih.gov/gene/2674).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Concentrated ammonium sulphate in PBS pH 7.4 containing no preservatives.
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Recombinant His-tagged human GFR alpha-1 protein produced using CHO-based cell line. For production of hGFR alpha-1, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
WB, ICC. A dilution of 1:5,000 to 1:10,000 is recommended for Western blot and 1:1,000 to 1:2,000 for Immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
RET ligand 1;TGF-beta related neurotrophic factor receptor 1; GDNFR-alpha-1; GFR-alpha-1; TRNR1; RETL1; GFRA1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human GFR alpha-1. No cross reaction with hGFR alpha-2, hGFR alpha-3, hGFR alpha-4
Storage:
Store at 2-8°C upon receipt; DO NOT FREEZE. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use. See reconstitution instructions for more information.
Chicken anti-mCherry Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
mCherry is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals). The mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28 kDa protein similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome. The original DsRed was engineered extensively in the Tsien lab to prevent it from forming tetramers and dimers and to modify and improve the spectral properties (1-3). The resulting monomeric protein is useful for applications such as Foerster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). Several further cycles of mutation, directed modification and evolutionary selection produced mCherry, which is monomeric and has an excitation maximum at 587 nm and and emission maximum at 610 nm (4).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Species Independent
Immunogen:
Recombinant full length mCherry.
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:2,000 - 1:5,000 isC recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a band at ~28-30 kDa corresponding to intact full-length mCherry by Western blot on HEK293 cells transfected with mCherry vector. It has also been used successfully for immunocytochemistry.
Storage:
Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.
Chicken anti-Growth associated protein 43 (GAP-43) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
GAP43 is very abundant protein which is found concentrated in neurons. One group discovered it as one of three proteins which becomes unregulated during the regeneration of the toad optic nerve (1). Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 were independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14 kDa. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Bovine,Human,Other Mammals (Predicted),Rat
Immunogen:
C-terminal peptide 217-227 of rat and mouse GAP43, which is KEDPEADQEHA, to which an N terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein.
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:1,000 - 1:5,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
neuron growth-associated protein 43; neuromodulin; nerve growth-related peptide GAP43; axonal membrane protein GAP-43; protein F1; calmodulin-binding protein P-57; neural phosphoprotein B-50; Growth Associated Protein 43; GAP43;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a 43 kDa band by Western blot on bovine hippocampus lysate. It has also been used successfully for immunocytochemistry. Human, rat and bovine. It is expected that it will work on other mammal tissues due to amino acid sequence similarity.
Storage:
Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two mouse beta NGF peptides (74-85 and 113-124 aa).
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
ELISA, WB and IHC. WB suggested dilution of 1:500-1:2,000. IHC suggested dilution of 1:50-1:1,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Beta-nerve growth factor; Beta-NGF; NGF; NGFB;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human beta NGF, cross reactivity to other species NGF is expected
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Dicer is the rate limiting enzyme in the formation of mature microRNAs.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, 0.02% sodium azide
Host Animal:
Chicken
Species Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide from mouse Dicer protein (1388-1405 aa) conjugated to KLH.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunohistochemistry (IHC) and Immunoprecipitation (IP). The recommended concentration for IHC in formalin fixed and paraffin embedded tissues and formalin/acetone fixed tissues is 1:200-1:500. For WB, the recommended concentration is 1:1,000 to 1:3,000. Mouse Dicer Protein has a predicted length of 1916 residues and the MW of the monomer is 218 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Lugli G. et al (2008) Expression of microRNAs and their precursors in synaptic fractions of adult mouse forebrain. J Neurochem. Jul;106(2):650-61. Lugli G. et al (2005) Dicer and eIF2c are enriched at postsynaptic densities in adult mouse brain and are modified by neuronal activity in a calpain-dependent manner. J Neurochem. Aug;94(4):896-905.
Specificity:
Specificity confirmed by IHC and WB in mouse brain. Mouse; Rat;
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Chicken anti-Mitochondrial uncoupling protein 3 (UCP 3) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Uncoupling Protein 3 (UCP3) belongs to the mitochondrial carrier family. Located in the mitochondrion inner membrane, UCP3 creates proton leaks across the membrane thus uncoupling oxidative phosphorylation (Ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Pig,Rat
Immunogen:
A peptide from the C-terminus of human Uncoupling Protein 3 (298-312 aa).
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution at 1:1,000 to 1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
UCP3; Mitochondrial uncoupling protein 3; UCP 3; Solute carrier family 25 member 9; SLC25A9;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody detects UCP3 at approx 35 kDa. Human, Mouse, Rat, Porcine
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Transforming growth factor beta-1 (TGFB1) is a multi-functional cytokine with roles in proliferation, differentiation and other functions in many cell types. The secreted TGFB1 protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide (Ref: SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Pig,Rat
Immunogen:
A peptide from human and mouse Transforming growth factor beta-1 (372-386 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
TGFB1; TGFB; TGF-beta-1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
TGF beta 1, cross reactivity to other species is expected
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-C-reactive protein (CRP) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
C-reactive protein has several roles associated with host defence such as; promoting agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. COFACTOR: Binds 2 calcium ions per subunit. C-reactive protein exists as a homopentamer. There are 2 alternatively spliced isoforms. C-reactive protein is found in plasma and its concentration increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL-1 and IL-6.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Rat
Immunogen:
A peptide from rat C-reactive protein (108-121 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
CRP; PTX1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Rat C-reactive protein
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Peptide YY (PYY) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Peptide YY (PYY) is secreted from endocrine cells in the lower small intestine, colon and pancreas. PYY inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility (Ref: SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from the N-terminus of human mature Peptide YY (3-18 aa).
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:2,000 to 1:10,000 (detection antibody). Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Peptide YY (PYY) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Peptide YY (PYY) is secreted from endocrine cells in the lower small intestine, colon and pancreas. PYY inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility (Ref: SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from the N-terminus of human mature Peptide YY (3-18 aa).
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:200 to 1:1,000 (coating antibody) and 1:2,000 to 1:5,000 (detection antibody) .
Chicken anti-Peptide YY (PYY) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Peptide YY (PYY) is secreted from endocrine cells in the lower small intestine, colon and pancreas. PYY inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility (Ref: SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from the C-terminus of human mature Peptide YY (24-36 aa) .
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:2,000 to 1:10,000 (detection antibody). Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Peptide YY (PYY) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Peptide YY (PYY) is secreted from endocrine cells in the lower small intestine, colon and pancreas. PYY inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility (Ref: SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from the C-terminus of human mature Peptide YY (24-36 aa) .
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA. Suggested dilution at 1:200 to 1:1,000 (coating antibody) and 1:2,000 to 1:5,000 (detection antibody). Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Presenilin-2 (PS-2) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Presenilin-2 (PSEN2) is a multi-pass membrane protein and component of the gamma-secretase complex. Defects in PSEN2 are a cause of Alzheimer disease type 4 (AD4), an autosomal dominant Alzheimer disease. (Ref:SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two human Presenilin 2 peptides (319-330 and 349-360 aa). Both sequences are highly conserved in human, mouse and rat.
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:1,000 to 1:4,000. To minimise background staining, a higher concentration of detergent (such as Tween-20) is suggested in the dilution and washing steps. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Presenilin-1 (PS-1) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Presenilin-1 (PSEN1) is a multi-pass membrane protein and component of the gamma-secretase complex. PSEN1 is thought to play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. It may also play a role in hematopoiesis. Defects in PSEN1 are a cause of Alzheimer disease type 3 (AD3), a familial early-onset form of Alzheimer disease (Ref:SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two human Presenilin 1 peptides (311-322 and 341-352 aa). Both sequences are highly conserved in human, mouse and rat.
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:1,000 to 1:4,000. To minimise background staining, a higher concentration of detergent (such as Tween-20) is suggested in the dilution and washing steps. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Presenilin 1; PS-1; Protein S182; PS1-CTF12; PSEN1; AD3; PS1; PSNL1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human Presenilin 1
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Peroxisome proliferator-activated receptor gamma (PPARG) is a nuclear hormone receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription (Ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Human Peroxisome proliferator-activated receptor gamma peptide (115-129 aa).
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution at 1:2,000 to 1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
PPAR gamma; PPAR-gamma; Nuclear receptor subfamily 1 group C member 3; PPARG; NR1C3;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human Peroxisome proliferator-activated receptor gamma
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Orexin B Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
FUNCTION: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. SUBCELLULAR LOCATION: Endoplasmic reticulum; rough endoplasmic reticulum. Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses. SIMILARITY: Belongs to the orexin family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from the N-terminus of human Orexin B (1-14 aa).
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:2,000 to 1:5,000 as a detection antibody and 1:200 to 1:1,000 as a coating antibody in sandwich ELISA. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Orexin A Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
FUNCTION: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. SUBCELLULAR LOCATION: Endoplasmic reticulum; rough endoplasmic reticulum. Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses. SIMILARITY: Belongs to the orexin family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide from the N-terminus of human Orexin A (1-13 aa) .
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:2,000 to 1:5,000 as a detection antibody and 1:200 to 1:1,000 as a coating antibody in sandwich ELISA. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Obestatin Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Obestatin is generated from the proteolytic cleavage of Ghrelin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Mouse,Rat
Immunogen:
Obestatin peptide (76-98 aa) from mouse ghrelin precursor.
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:500 to 1:2,000 in coating buffer as coating antibody in competitive ELISA testing. Biosensis recommends that the optimal working dilution should be determined by the end user.
Beta-adrenergic receptors are multi-pass membrane proteins that belong to the G-protein coupled receptor 1 family. Their function is to mediate the catecholamine-induced activation of adenylate cyclase through the action of G-proteins (ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide from mouse beta 3 Adrenergic Receptor (386-400 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Fatty acid-binding protein, adipocyte (ALBP) is a lipid transport protein which binds long chain fatty acids and other hydrophobic ligands and delivers them to their receptors in the nucleus. ALBP is found in the cytoplasm and nucleus of adipocytes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two human Fatty acid-binding protein, adipocyte peptide peptides (71-82 aa, 97-111 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:1,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Fatty acid-binding protein, adipocyte (ALBP) is a lipid transport protein which binds long chain fatty acids and other hydrophobic ligands and delivers them to their receptors in the nucleus. ALBP is found in the cytoplasm and nucleus of adipocytes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Human Fatty acid-binding protein, adipocyte peptide (97-111 aa).
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
CNTF is a survival promoting factor for different types of neurons in vitro and in vivo. The essential structural features for the biological function of human CNTF were investigated by Thier, M. et al. They showed that deletion of 14 N-terminal and 18 C-terminal amino acids significantly increased bioactivity compared to wild-type CNTF. FUNCTION: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Nervous system. PHARMACEUTICAL: CNTF is being tested under the name Axokine by Regeneron Pharmaceuticals for treatment of human motor neuron diseases, such as amyotrophic lateral sclerosis (ALS). As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. SIMILARITY: Belongs to the CNTF family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two peptides from human CNTF (11-25 and 186-200 aa). Both peptides are highly conserved among human and mouse.
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA at a suggested dilution of 1:500 to 1:2,000. A titration between 1:50 to 1:500 is suggested for IHC. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Ciliary neurotrophic factor; CNTF;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
CNTF, Cross reactivity to other species is expected
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Appetite-regulating hormone, active (Active Ghrelin) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) and upon binding to the receptor it induces the release of growth hormone from the pituitary. This ligand has an appetite-stimulating effect and is involved in growth regulation (Ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Human active Ghrelin peptide (24-33 aa) S3n-octanoicacid covalently linked to amino acid 28
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:500 to 1:4,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Adiponectin Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Adiponectin is synthesized by adipocytes and is involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities (ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide from human Adiponectin (230-244 aa).
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Hormone-sensitive lipase (HSL) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Hormone Sensitive Lipase (HSL) hydrolyzes stored triglycerides to free fatty acids in adipose tissue and heart. In steroidogenic tissues, HSL principally converts cholesteryl esters to free cholesterol for steroid hormone production (ref: SWISSPROT).
WB. Suggested dilution at 1:1,000 to 1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
HSL; LIPE; Hormone-sensitive lipase; Lipe;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody detects HSL at approx 83 kDa in various fat cell lysates from mouse and rat. Additional weaker band may appear at approx 40 kDa (unknown). Mouse, Rat, Human
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Hormone-sensitive lipase (HSL) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Hormone Sensitive Lipase (HSL) hydrolyzes stored triglycerides to free fatty acids in adipose tissue and heart. In steroidogenic tissues, HSL principally converts cholesteryl esters to free cholesterol for steroid hormone production (ref: SWISSPROT).
Chicken anti-Rhodopsin Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Rod shaped photoreceptor cells that are required for image-forming vision at low light intensity and for photoreceptor cell viability after birth (ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Purified chicken IgY. Liquid in PBS with 0.02% Sodium Azide. Concentration 1 mg/mL.
Host Animal:
Chicken
Species Reactivity:
Mouse,Rat,Tiger Finch,Zebra Finch
Immunogen:
Mixture of synthetic peptide (14-26 aa) of Mouse Green cones Opsin 1 and synthetic peptide (18-30 aa) of Tiger finch red cones opsin 1 was used as immunogen.
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution at 1:500 to 1:2,000. The antibody recognizes a band of approximately 37 kD on mouse brain lysate. To minimise background staining, a higher concentration of detergent (such as Tween-20) is suggested in the dilution and washing steps. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Rho; Green cone; Red cone; opsin1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Mouse, Rat, Tiger Finch, Zebra finch
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-G-Protein B3 (Rho) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
G-Protein b3 (GNB3) is a guanine nucleotide-binding protein (G protein). G-proteins are involved as a modulator or transducer in various transmembrane signaling systems (Ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Human GNB3 peptide (216-230 aa)
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution at 1:1,000 to 1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Appetite-regulating hormone (Grehlin) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) and upon binding to the receptor it induces the release of growth hormone from the pituitary. This ligand has an appetite-stimulating effect and is involved in growth regulation (Ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic peptide from human Ghrelin, C-terminal, (17-28 aa)
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA. Suggested dilution at 1:500 to 1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two human GDNF peptides (101-118 and 199-211 aa). Both peptides are highly conserved in human and mouse.
Applications:
ELISA,ICC,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
ELISA, WB and IHC. WB suggested dilution of 1:500-1:2,000. IHC suggested dilution of 1:50-1:500. Biosensis recommends that the optimal working dilution should be determined by the end user.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse
Immunogen:
Mixture of two human BDNF peptides (73-87 and 194-208 aa). Both peptides are highly conserved in human and mouse.
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA. Suggested dilution at 1:500 to 1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Acetyl-lysine Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Lysine acetylation of histones and non-histone proteins plays an important part in many cellular processes such as chromatin and nuclear signaling, transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication and repair.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Species Independent
Immunogen:
Acetylated Lysine OVA
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
ELISA and WB. Suggested dilution of 1:1,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Acetyl-lysine;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Binds to proteins with acetylated lysine residues.
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Leptin Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Leptin is secreted by white adipocytes and functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. Leptin also has several endocrine functions and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing (Ref: Entrez Gene) .
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Mouse,Rat
Immunogen:
Recombinant rat Leptin protein
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
ELISA and WB. Suggested dilution of 1:1,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Leptin; Obesity factor; Lep; Ob; Obese protein;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Rat Leptin
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Neurotrophin-4/5 (NT-4/5) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse
Immunogen:
Mixture of two human NT4/NT5 peptides (104-118 and 198-210 aa).
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA at suggested dilution of 1:500-1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Neurotrophin-3 (NT-3) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
FUNCTION: Seems to promote the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse
Immunogen:
Mixture of two human NT3 peptides (90-104 and 199-214 aa).
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA at suggested dilution of 1:500-1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-C-reactive protein (CRP) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
C-reactive protein has several roles associated with host defence such as; promoting agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. COFACTOR: Binds 2 calcium ions per subunit. C-reactive protein exists as a homopentamer. There are 2 alternatively spliced isoforms. C-reactive protein is found in plasma and its concentration increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL-1 and IL-6.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Mouse
Immunogen:
A peptide from the C-terminus of mouse C-reactive protein (210-225 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
ELISA and WB. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
CRP; PTX1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
C-reactive protein Mouse
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Leptin Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Leptin is secreted by white adipocytes and functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. Leptin also has several endocrine functions and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing (Ref: Entrez Gene).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Recombinant human Leptin protein
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
ELISA and WB. Suggested dilution of 1:1,000-1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Leptin; Obesity factor; Lep; Ob; Obese protein;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human Leptin
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-C-reactive protein (CRP) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
C-reactive protein has several roles associated with host defence such as; promoting agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. COFACTOR: Binds 2 calcium ions per subunit. C-reactive protein exists as a homopentamer. There are 2 alternatively spliced isoforms. C-reactive protein is found in plasma and its concentration increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL-1 and IL-6.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Purified human C-reactive protein
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
ELISA and WB. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
CRP; PTX1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human C-reactive protein Human
Storage:
Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.
Chicken anti-Vimentin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
Vimentins are class-III intermediate filaments specific to mesenchymal tissue. Vimentin is an important cytoskeletal component responsible for maintaining cell integrity and has a probable role in the intracellular transport of proteins such as lipoproteins between the nucleus and plasma membrane. Immunohistochemical staining for Vimentin is characteristic of sarcomas.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human Vimentin purified from E.coli
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000-5,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
VIM;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody detects ~50 kDa Vimentin enzyme. Hu, Rat, Ms. It is predicted to react with other mammals due to sequence homology.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Recombinant full length human Ubiquitin C Terminal Hydrolase 1 (UCHL1) purified from E. coli.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord or peripheral nerve homogenate. Hu, Rat, Ms, Bov, Por. Predicted to react with other mammalian tissues due to sequence homology.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Peripherin is a class-III neuronal intermediate filament protein found in certain classes of neuron, most of which are located in the peripheral nervous system.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Cat,Human,Mouse,Other Mammals,Rat
Immunogen:
Recombinant full length rat Peripherin protein expressed in and purified from E.coli
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Peripherin; Prph; Prph1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Sekerkova G. et al (2008) Espin actin-cytoskeletal proteins are in rat type I spiral ganglion neurons and include splice-isoforms with a functional nuclear localization signal. J Comp Neurol. 2008 Aug 20;509(6):661-76.
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody detects ~57 kDa Peripherin protein. A suitable control tissue is rat spinal cord or peripheral nerve homogenate. Hu, Rat, Ms, Fel, and other mammals
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
The enzyme Peptidylprolyl isomerase (Pin1) is responsible for flipping the proline ring from the cis to trans conformation. This enzyme regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity (ref: SWISSPROT). Pin1 is concentrated in the nucleus in small punctate structures and is particularly obvious in tumor cells.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Cat,Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant full length Peptidylprolyl isomerase (Pin1) purified from E.coli
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB. This antibody detects ~21 kDa Pin1 protein. Human, Rat, Mouse and Feline. Predicted to react with other mammalian tissue.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Chicken anti-Neurofilament medium polypeptide (NF-M) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Recombinant fusion protein containing the extreme C-terminal segment of rat NF-M.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:2,000 is recommended for ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Jarjour A.A. et al (2007) Maintenance of axo-oligodendroglial paranodal junctions requires DCC and netrin-1. J Neurosci. 2008 Oct 22;28(43):11003-14. Rangaraju S. et al (2009) Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging. Aging Cell. 2009 Apr;8(2):178-91. Pearse D.D. et al (2007) Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery. Glia. 2007 Jul;55(9):976-1000. Shaw G. et al (2004) Characterization of the bovine neurofilament NF-M protein and cDNA sequence, and identification of in vitro and in vivo calpain cleavage sites. Biochem Biophys Res Commun. 2004 Dec 10;325(2):619-25.
Specificity:
Specifically recognizes the medium neurofilament subunit NF-L in WB. Band appears at ~145 kDa in WB from rodent and ~160 kDa for human and bovine WB. Hu, Rat, Ms, Fel, Chk. Predicted to react with other mammalian tissues due to sequence homology.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Purified porcine NF-L from spinal cord and recombinant NF-L.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:5,000 is recommended for ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rangaraju S. et al (2009) Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging. Aging Cell. 2009 Apr;8(2):178-91.
Specificity:
The specificity of this antibody has been confirmed by IC. Hu, Rat, Ms, Fel, Chk. Predicted to react with other mammalian tissues due to sequence homology.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Chicken anti-Neurofilament heavy polypeptide, phosphorylated (pNF-H) Polyclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
Background Info:
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. This antibody binds primarily to the phosphorylated axonal forms of NF-H.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). Suggested dilution for WB is 1:20,000-50,000. Suggested dilution for ICC/IHC is 1:20,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Jarjour A.A. et al (2007) Maintenance of axo-oligodendroglial paranodal junctions requires DCC and netrin-1. J Neurosci. 2008 Oct 22;28(43):11003-14. Pearse D.D. et al (2007) Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery. Glia. 2007 Jul;55(9):976-1000. Shaw G. et al (2005) Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury. Biochem Biophys Res Commun. 2005 Nov 4;336(4):1268-77.
Specificity:
This antibody reacts with phosphorylated NF-H and is seen as a band of approx 200 kDa in WB. Refer to publication by Shaw et al (2005) for the use of this antibody in an ELISA to detect NF-H. Hu, Rat, Ms, Fel, Chk. Predicted to react with other mammalian tissues due to sequence homology.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Purification:
IgY
Target:
Neurofilament heavy polypeptide, phosphorylated (pNF-H)
Chicken anti-Myelin basic protein (MBP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of approximately 80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP). MBP is an 'intrinsically unstructured' protein with a high proportion (approximately 75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system. A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational. Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic. The message for myelin basic protein is selectively translocated to the ends of the cell processes. Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model. FUNCTION: Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity). SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Myelin membrane; peripheral membrane protein; cytoplasmic side. Cytoplasmic side of myelin. TISSUE SPECIFICITY: Found in both the central and the peripheral nervous system.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Bovine,Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Three peptide sequences conserved in higher verterbrate MBP protein.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). The recommended dilution for WB is 1:5,000-10,0000 and 1:500-1,000 for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rangaraju S. et al (2009) Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging. Aging Cell. 2009 Apr;8(2):178-91.
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody stains a prominent band at approx. 20 kDa. A suitable control tissue is rat spinal cord or peripheral nerve homogenate. The major isoforms of MBP run as a closely spaced double of 22 kDa and 18 kDa. Hu, Rat, Ms, Bov. Predicted to react with other mammalian tissues due to sequence homology.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Chicken anti-Microtubule-associated protein 2 (MAP2) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Bovine,Human,Mouse,Rat
Immunogen:
Bovine MAP2 isolated from brain by the GTP microtubule cycling method.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). Suggested dilutions are: 1:10,00-1:20,000 (WB), 1:5,000-1:10,000 (ICC, IHC). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Microtubule-associated protein 2; MAP-2; MAP2;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Michurina A (2021) "Loss of Setd1b methyltransferase in the murine forebrain as a novel model for human intellectual disability." PhD Thesis. Application: IHC (IF) Species: Mouse. Iwata M et al. (2019) "Regulatory mechanisms for the axonal localization of tau protein in neurons." Mol Biol Cell. 30(19):2441-57. Application: ICC/IF Species: Mouse, rat. Duda JK et al. (2019) "The role of DLG-MAGUKs in mediating signaling specificity at the postsynaptic density." PhD Thesis. [Epub ahead of print]. Application: ICC/IF Species: Mouse. Awashti A et al. (2018) "Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting." Science. 2018; [Epub ahead of print]. Application: IHC/ICC/IF Species: Mouse. Wolfes AC et al. (2016) "A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes." J Gen Physiol. 2016; [Epub ahead of print]. Application: IF Species: Rat. Dziennis S et al. (2007) "Role of signal transducer and activator of transcription-3 in estradiol-mediated neuroprotection." J Neurosci. 2007; 27(27):7268-74. Application: IHC Species: Rat.
Specificity:
The specificity of this antibody has been confirmed by IC. Hu, Rat, Ms, Bov
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Alpha-internexin is a ~66 kDa Class IV intermediate filament subunit expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Cat,Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant rat alpha-internexin expressed and purified from E. coli
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:5,000 - 10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB. This antibody is specific for the 64-66 kDa alpha-internexin protein. Molecular weight will depend on species. Human, Rat, Mouse, Feline. It is predicted to react with other mammals.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Chicken anti-Glial fibrillary acidic protein (GFAP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded, IHC-Frozen, ICC.
Background Info:
Glial fibrillary acidic protein (GFAP) is approx. 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Cat,Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant GFAP (expressed in E.coli) and native bovine GFAP
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). WB: A dilution of 1:5,000 is recommended. ICC: A dilution of 1:1,000-1:5,000 using fluorescent secondary antibodies or peroxidase or other enzyme-linked methods is recommended on 4% PFA fixed cells in culture, with 3hr-o/n incubation of primary antibody. IHC: 4% PFA frozen tissues, permeabilized. IHC (paraffin-embedded): capable, HEIR treatment typically necessary. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Astrocyte; Glial fibrillary acidic protein; GFAP;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Hascup KN et al. (2020) Riluzole attenuates glutamatergic tone and cognitive decline in A?PP/PS1 mice. J Neurochem. [Epub ahead of print] Application: Mouse, IHC(IF).
Specificity:
The specificity of this antibody has been confirmed by WB. Human, Rat, Mouse, Feline. Predicted to react with other mammals.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Chicken anti-Casein kinase I isoform alpha (CKI-alpha) Polyclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
Background Info:
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration. (Reference: uniprot.org)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Bovine,Chicken,Horse,Human,Mouse,Pig,Rat
Immunogen:
Shortest isoform of recombinant full length CK1a.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB): 1:5,000 - 1:10,000. Casein kinase 1 alpha has a predicted molecular weight of 38 kDa. <br><br>Immunocytochemistry (ICC): 1:500-1:1,000.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CK1; CKI-alpha;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Antibody recognizes CK1 by western blot and immunohistochemistry. Cross reactivity
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
BL-005-500 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing mouse immunoreagents. Following ELISA assays in the Biosensis Rapid TM ELISA range have been validated to achieve accurate results using BL-005-500: BEK-2212, Human NGF; Application: Citrate-Plasma One vial of BL-005-500 contains 500 ?g IgG which is sufficient as sample diluent additive for one 96-well plate. Other ELISA assays that use mouse assay antibodies may also benefit from addition of blocker BL-005-500, but require optimization of working concentration and assay validation for accurate results.
Product Type:
Immunoassay Blocker
Format:
Lyophilized.
Species Reactivity:
Human
Applications:
ELISA
Application Details:
Immunoassay blocker to reduce or eliminate heterophilic antibody interference in sandwich ELISA assays for validated sample applications.
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Store unopened vial at 2-8°C.
Purification:
Purified
Target:
Heterophilic antibody immunoassay blocker for BEK-2212 and similar ELISA assays
BL-004-500 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing sheep immunoreagents. Following ELISA assays in the Biosensis Rapid TM ELISA range have been validated to achieve accurate results using BL-004-500: BEK-2217/2240, Human proBDNF; Application: Serum, Citrate-Plasma BEK-2221, Human NT3; Application: Plasma (Citrate and EDTA) BEK-2237, Human proBDNF; Application: Serum, EDTA-Plasma One vial of BL-004-500 contains 500 ?g IgG which is sufficient as sample diluent additive for one 96-well plate. Other ELISA assays that use sheep assay antibodies may also benefit from addition of blocker BL-004-500, but require optimization of working concentration and assay validation for accurate results.
Product Type:
Immunoassay Blocker
Format:
Lyophilized.
Species Reactivity:
Human
Applications:
ELISA
Application Details:
Immunoassay blocker to reduce or eliminate heterophilic antibody interference in sandwich ELISA assays for validated sample applications.
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Store unopened vial at 2-8°C.
Purification:
Purified
Target:
Heterophilic antibody immunoassay blocker for BEK-2217, BEK-2221, BEK-2237 & BEK-2240 and similar ELISA assays
BL-003-1000 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing a combination of sheep and mouse immunoreagents. Following ELISA assays in the Biosensis Rapid TM ELISA range have been validated to achieve accurate results using BL-003-1000: BEK-2226, Human proNGF; Application: Serum, Heparin-Plasma BEK-2218, Human NT4/5; Application: Citrate-Plasma One vial of BL-003-1000 contains 1000 ?g IgG which is sufficient as sample diluent additive for one 96-well plate. Other ELISA assays that use sheep and mouse assay antibodies may also benefit from addition of blocker BL-003-1000, but require optimization of working concentration and assay validation for accurate results.
Product Type:
Immunoassay Blocker
Format:
Lyophilized.
Species Reactivity:
Human
Applications:
ELISA
Application Details:
Immunoassay blocker to reduce or eliminate heterophilic antibody interference in sandwich ELISA assays for validated sample applications.
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Store unopened vial at 2-8°C.
Purification:
Purified
Target:
Heterophilic antibody immunoassay blocker for BEK-2226 & BEK-2218 and similar ELISA assays
BL-002-1250 contains a proprietary mixture of proteins and buffer reagents designed to reduce heterophilic interactions in ELISA assays utilizing mouse immunoreagents. One vial of BL-002-1250 contains 1250 ?g IgG which is sufficient as sample diluent additive for one 96-well plate if used at 50 ?g/mL. Optimal blocker concentration needs to be validated by the end-user for accurate results.
Product Type:
Immunoassay Blocker
Format:
Lyophilized.
Species Reactivity:
Human
Applications:
ELISA
Application Details:
Immunoassay blocker to reduce or eliminate heterophilic antibody interference in sandwich ELISA assays utilizing mouse antibodies.
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Store unopened vial at 2-8°C.
Purification:
Purified
Target:
Heterophilic antibody immunoassay blocker for BEK-2219 and similar ELISA assays
BL-001-1250 contains a proprietary mixture of proteins and buffer reagents designed to reduce the sIgA cross-reactivity present in certain substrates such as milk. Following ELISA assays in the Biosensis Rapid TM ELISA range have been validated to achieve accurate results using BL-001-1250: BEK-2211, Mature BDNF; Application: human milk One vial of BL-001-1250 contains 1250 ?g of proprietary immunoglobulins, which is sufficient as sample diluent additive for one 96-well plate. Other ELISA assays may also benefit from addition of blocker BL-001-1250, but require optimization of working concentration and assay validation for accurate results.
Product Type:
Immunoassay Blocker
Format:
Lyophilized.
Species Reactivity:
Human
Applications:
ELISA
Application Details:
Immunoassay blocker to reduce or eliminate secretory IgA (sIgA) interference in sandwich ELISA assays for validated sample applications.
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Store unopened vial at 2-8°C.
Purification:
Purified
Target:
Secretory IgA immunoassay Blocker for BEK-2211 and similar ELISA assays
?-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are ?-Casein A1 and ?-Casein A2. The two types of ?-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A2 ?-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A2 ?-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-bovine ?-Casein polyclonal capture antibody, a chicken anti-bovine A2 ?-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A2 ?-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A2 ?-Casein in full cream milk, skim milk and reconstituted A2 milk powder. Please refer to the kit protocol for specific use instructions. The A1 isoform is not detected in this ELISA assay.
Product Type:
ELISA Assay
Species Reactivity:
Bovine
Immunogen:
Purified bovine beta Casein and peptide specific peptides for A2
Applications:
ELISA
Application Details:
ELISA. For the quantification of A2 Beta casein (A2) in Bovine Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
CSN2; Bovine A2 Beta Casein; A2;
Biosensis Brand:
Biosensis®
Detection Method:
Colorimetric
Shelf Life:
12 months after date of receipt unopened.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer, stop solution and detailed protocols.
Specificity:
Bovine A2 beta-casein
Storage:
Store at 2-8°C
Range:
3.1 - 200 ng/mL
Sample Type:
Bovine Milk
Sensitivity:
This bovine A2 beta-casein ELISA kit detects a minimum of 2 ng/mL A2 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10)).
Cross Reactivity:
No cross-reactivity is observed with bovine A1 beta-Casein.
?-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are ?-Casein A1 and ?-Casein A2. The two types of ?-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A2 ?-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A2 ?-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-bovine ?-Casein polyclonal capture antibody, a chicken anti-bovine A2 ?-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A2 ?-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A2 ?-Casein in full cream milk, skim milk and reconstituted A2 milk powder. Please refer to the kit protocol for specific use instructions. The A1 isoform is not detected in this ELISA assay.
Product Type:
ELISA Assay
Species Reactivity:
Bovine
Immunogen:
Purified bovine beta Casein and peptide specific peptides for A2
Applications:
ELISA
Application Details:
ELISA. For the quantification of A2 Beta casein (A2) in Bovine Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
CSN2; Bovine A2 Beta Casein; A2;
Biosensis Brand:
Biosensis®
Detection Method:
Colorimetric
Shelf Life:
12 months after date of receipt unopened.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer, stop solution and detailed protocols.
Specificity:
Bovine A2 beta-casein
Storage:
Store at 2-8°C
Range:
3.1 - 200 ng/mL
Sample Type:
Bovine Milk
Sensitivity:
This bovine A2 beta-casein ELISA kit detects a minimum of 2 ng/mL A2 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10)).
Cross Reactivity:
No cross-reactivity is observed with bovine A1 beta-Casein.
?-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are ?-Casein A1 and ?-Casein A2. The two types of ?-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A2 ?-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A2 ?-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-bovine ?-Casein polyclonal capture antibody, a chicken anti-bovine A2 ?-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A2 ?-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A2 ?-Casein in full cream milk, skim milk and reconstituted A2 milk powder. Please refer to the kit protocol for specific use instructions. The A1 isoform is not detected in this ELISA assay.
Product Type:
ELISA Assay
Species Reactivity:
Bovine
Immunogen:
Purified bovine beta Casein and peptide specific peptides for A2
Applications:
ELISA
Application Details:
ELISA. For the quantification of A2 Beta casein (A2) in Bovine Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
CSN2; Bovine A2 Beta Casein; A2;
Biosensis Brand:
Biosensis®
Detection Method:
Colorimetric
Shelf Life:
12 months after date of receipt unopened.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer, stop solution and detailed protocols.
Specificity:
Bovine A2 beta-casein
Storage:
Store at 2-8°C
Range:
3.1 - 200 ng/mL
Sample Type:
Bovine Milk
Sensitivity:
This bovine A2 beta-casein ELISA kit detects a minimum of 2 ng/mL A2 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10))
Cross Reactivity:
No cross-reactivity is observed with bovine A1 beta-Casein.
?-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are ?-Casein A1 and ?-Casein A2. The two types of ?-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A2 ?-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A2 ?-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-bovine ?-Casein polyclonal capture antibody, a chicken anti-bovine A2 ?-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A2 ?-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A2 ?-Casein in full cream milk, skim milk and reconstituted A2 milk powder. Please refer to the kit protocol for specific use instructions. The A1 isoform is not detected in this ELISA assay.
Product Type:
ELISA Assay
Species Reactivity:
Bovine
Immunogen:
Purified bovine beta Casein and peptide specific peptides for A2
Applications:
ELISA
Application Details:
ELISA. For the quantification of A2 Beta casein (A2) in Bovine Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
CSN2; Bovine A2 Beta Casein; A2;
Biosensis Brand:
Biosensis®
Detection Method:
Colorimetric
Shelf Life:
12 months after date of receipt unopened.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer, stop solution and detailed protocols.
Specificity:
Bovine A2 beta-casein
Storage:
Store at 2-8°C
Range:
3.1 - 200 ng/mL
Sample Type:
Bovine Milk
Sensitivity:
This bovine A2 beta-casein ELISA kit detects a minimum of 2 ng/mL A2 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10))
Cross Reactivity:
No cross-reactivity is observed with bovine A1 beta-Casein
?-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are ?-Casein A1 and ?-Casein A2. The two types of ?-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A1 ?-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A1 ?-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-Bovine ?-Casein polyclonal capture antibody, a chicken anti-bovine A1 ?-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A1 ?-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A1 ?-Casein in UHT (Ultra High Temperature) treated milk or long life milk, organic pasteurized and homogenized full cream milk, fresh pasteurized and homogenized milk, cold-pressed raw milk (non-pasteurized, non-homogenized), and biodynamic full-cream whole milk. Please refer to the kit protocol for specific use instructions. The A2 isoform is not detected in this ELISA assay.
Product Type:
ELISA Assay
Species Reactivity:
Bovine
Immunogen:
Purified bovine beta Casein and peptide specific peptides for A1
Applications:
ELISA
Application Details:
ELISA. For the quantification of A1 Beta casein (A1) in Bovine Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
CSN2; Bovine A1 Beta Casein; A1;
Biosensis Brand:
Biosensis®
Detection Method:
Colorimetric
Shelf Life:
12 months after date of receipt unopened.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer, stop solution and detailed protocols.
Specificity:
Bovine A1 beta-casein
Storage:
Store at 2-8°C
Range:
3.1 - 200 ng/mL
Sample Type:
Bovine Milk
Sensitivity:
This bovine A1 beta-casein ELISA kit detects a minimum of 3 ng/mL A1 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10))
Cross Reactivity:
No cross-reactivity is observed with bovine A2 beta-Casein
?-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are ?-Casein A1 and ?-Casein A2. The two types of ?-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A1 ?-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A1 ?-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-Bovine ?-Casein polyclonal capture antibody, a chicken anti-bovine A1 ?-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A1 ?-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A1 ?-Casein in UHT (Ultra High Temperature) treated milk or long life milk, organic pasteurized and homogenized full cream milk, fresh pasteurized and homogenized milk, cold-pressed raw milk (non-pasteurized, non-homogenized), and biodynamic full-cream whole milk. Please refer to the kit protocol for specific use instructions. The A2 isoform is not detected in this ELISA assay.
Product Type:
ELISA Assay
Species Reactivity:
Bovine
Immunogen:
Purified bovine beta Casein and peptide specific peptides for A1
Applications:
ELISA
Application Details:
ELISA. For the quantification of A1 Beta casein (A1) in Bovine Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
CSN2; Bovine A1 Beta Casein; A1;
Biosensis Brand:
Biosensis®
Detection Method:
Colorimetric
Shelf Life:
12 months after date of receipt unopened.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer, stop solution and detailed protocols.
Specificity:
Bovine A1 beta-casein
Storage:
Store at 2-8°C
Range:
3.1 - 200 ng/mL
Sample Type:
Bovine Milk
Sensitivity:
This bovine A1 beta-casein ELISA kit detects a minimum of 3 ng/mL A1 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10)).
Cross Reactivity:
No cross-reactivity is observed with bovine A2 beta-Casein.
?-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are ?-Casein A1 and ?-Casein A2. The two types of ?-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A1 ?-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A1 ?-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-Bovine ?-Casein polyclonal capture antibody, a chicken anti-bovine A1 ?-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A1 ?-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A1 ?-Casein in UHT (Ultra High Temperature) treated milk or long life milk, organic pasteurized and homogenized full cream milk, fresh pasteurized and homogenized milk, cold-pressed raw milk (non-pasteurized, non-homogenized), and biodynamic full-cream whole milk. Please refer to the kit protocol for specific use instructions. The A2 isoform is not detected in this ELISA assay.
Product Type:
ELISA Assay
Species Reactivity:
Bovine
Immunogen:
Purified bovine beta Casein and peptide specific peptides for A1
Applications:
ELISA
Application Details:
ELISA. For the quantification of A1 Beta casein (A1) in Bovine Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
CSN2; Bovine A1 Beta Casein; A1;
Biosensis Brand:
Biosensis®
Detection Method:
Colorimetric
Shelf Life:
12 months after date of receipt unopened.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer, stop solution and detailed protocols.
Specificity:
Bovine A1 beta-casein
Storage:
Store at 2-8°C
Range:
3.1 - 200 ng/mL
Sample Type:
Bovine Milk
Sensitivity:
This bovine A1 beta-casein ELISA kit detects a minimum of 3 ng/mL A1 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10)).
Cross Reactivity:
No cross-reactivity is observed with bovine A2 beta-Casein.
?-Casein is expressed as 13 genetic variants resulting from single nucleotide polymorphisms (SNP) in the CSN2 gene. The most frequent genetic variants in western dairy breeds are ?-Casein A1 and ?-Casein A2. The two types of ?-Casein protein, A1 and A2, differ by a single-point mutation at amino acid position 82 (P82/H82). The Biosensis Bovine A1 ?-Casein enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the bovine A1 ?-Casein isoform in 5 hours. This kit consists of a pre-coated rabbit anti-Bovine ?-Casein polyclonal capture antibody, a chicken anti-bovine A1 ?-Casein detection antibody and a horseradish peroxidase (HRP)-conjugated donkey anti-chicken IgY antibody. The addition of a substrate (3,3',5,5' -tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of Bovine A1 ?-Casein present in samples and protein standards. Extensive validation has shown accurate quantification of A1 ?-Casein in UHT (Ultra High Temperature) treated milk or long life milk, organic pasteurized and homogenized full cream milk, fresh pasteurized and homogenized milk, cold-pressed raw milk (non-pasteurized, non-homogenized), and biodynamic full-cream whole milk. Please refer to the kit protocol for specific use instructions. The A2 isoform is not detected in this ELISA assay.
Product Type:
ELISA Assay
Species Reactivity:
Bovine
Immunogen:
Purified bovine beta Casein and peptide specific peptides for A1
Applications:
ELISA
Application Details:
ELISA. For the quantification of A1 Beta casein (A1) in Bovine Milk. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
CSN2; Bovine A1 Beta Casein; A1;
Biosensis Brand:
Biosensis®
Detection Method:
Colorimetric
Shelf Life:
12 months after date of receipt unopened.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer, stop solution and detailed protocols.
Specificity:
Bovine A1 beta-casein
Storage:
Store at 2-8°C
Range:
3.1 - 200 ng/mL
Sample Type:
Bovine Milk
Sensitivity:
This bovine A1 beta-casein ELISA kit detects a minimum of 3 ng/mL A1 beta-casein in assay buffer (defined as A1 concentration at blank OD plus 3x standard deviations of the blank OD (n=10))
Cross Reactivity:
No cross-reactivity is observed with bovine A2 beta-Casein
The Biosensis Mature BDNF/proBDNF Combo Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit combines individual, but complementary ELISA kits for the two most important BDNF isoforms: Mature BDNF ( BEK-2211 ) and full-length proBDNF ( BEK-2237 ). Both kits are sandwich ELISAs, useful for the quantification of mature BDNF and proBDNF in less than 4 hours in cell culture supernatants, serum, plasma (citrate and EDTA), mouse and rat cell lysates and rat brain extracts only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Please refer to the most current kit protocols for BEK-2211 (Mature BDNF Rapid TM ELISA) and BEK-2237 (proBDNF Rapid TM ELISA), for specific use instructions for each substrate application, in particular blood samples. BEK-2241 is the preferred Combo kit for human blood samples. The Mature BDNF ELISA kit consists of a pre-coated mouse monoclonal anti-mature BDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The proBDNF ELISA kit consists of a pre-coated monoclonal anti-proBDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature BDNF or proBDNF present in samples and protein standards. A BDNF and proBDNF positive control (QC sample) is provided to assure consistent assay performance. Both ELISA kits contain high quality protein calibrators. The Mature BDNF ELISA kit employs a recombinant E.coli-derived human mature BDNF standard approved by the World Health Organization (WHO, www.nibsc.org). The amino acid sequence of mature BDNF is identical for human, mouse, rat and a number of other species. This kit therefore is suitable to measure mature BDNF in all these species and uses the same antibodies and antigen. The proBDNF ELISA kit employs a recombinant mammalian, cleavage-resistant human proBDNF standard produced by Biosensis and validated against externally available proBDNF proteins. Due to a high degree of amino acid sequence homology, mouse and rat proBDNF can be quantified and expressed as human proBDNF equivalents. Internal Biosensis validation suggests that the use of the human standard provided in this kit will provide estimates that are identical, or close, to the actual levels of rat and mouse proBDNF present in rodent samples. Note that accurate proBDNF quantification in human serum and citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately. This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
See BEK-2211 & BEK-2237 for specific details
Applications:
ELISA
Application Details:
ELISA. For the quantification of Mature BDNF and proBDNF in Culture Supernatant, Serum, Plasma (Citrate), Plasma (EDTA), Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Brain-derived neurotrophic factor; Abrineurin
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months after date of receipt unopened.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 2 x 96-well pre-coated strip plates per kit (1 x BDNF antibody, 1 x proBDNF antibody coated plate), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Please refer to BEK-2211 (Mature BDNF Rapid TM ELISA Kit) and BEK-2237 (proBDNF Rapid TM ELISA Kit) .
Specificity:
Mature BDNF ELISA: Human, mouse, rat BDNF and numerous other species. Although the BDNF assay antibodies are raised against the mature domain, the mature BDNF ELISA preferentially detects mature BDNF over full-length proBDNF.
proBDNF ELISA: Human, mouse and rat proBDNF. The capture antibody used in the proBDNF ELISA kit binds to epitopes within the pro-domain of proBDNF. Thus, this ELISA detects the full length and potentially truncated form of proBDNF, and does not quantify mature BDNF.
Typical limit of detection (LOD) for mature BDNF is less than 2 pg/mL, and 6 pg/mL for proBDNF determined as 150% of the blank value.
Cross Reactivity:
<b>Cross-reactivity of BDNF isoforms:</b><br><br><b>Mature BDNF ELISA:</b><br>No cross-reactivity is observed for nerve growth factor (NGF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL in assay buffer. The reactivity of full-length proBDNF (0.125 ng/mL - 5 ng/mL) was determined in six independent assays using proBDNF proteins from four different sources (mammalian and bacterial, wild-type and mutated). The average cross-reactivity of proBDNF was found to be 5.3% +/- 0.5% in weight (w/v) concentration, or 12.1% +/- 1.2% in molar concentration (mean +/- SEM).<br><br><b>proBDNF ELISA:</b><br>Mature BDNF cross-reactivity was assessed by spiking 28 kDa mature BDNF protein obtained from WHO (www.nibsc.org) into a 1/5 diluted human serum sample at 5 ng/mL, which represents a BDNF concentration level of 25 ng/mL in undiluted, normal human serum. Cross-reactivity of mature BDNF was < 0.3% (w/v), or < 0.1% in molar concentration. The assay antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
The Biosensis Mature BDNF/proBDNF Combo Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit combines individual, but complementary ELISA kits for the two most important BDNF isoforms: Mature BDNF ( BEK-2211 ) and full-length proBDNF ( BEK-2217 ). Both kits are sandwich ELISAs, useful for the quantification of mature BDNF and proBDNF in less than 4 hours in cell culture supernatants, serum, plasma (citrate), cell lysates and brain extracts only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Please refer to the most current kit protocols for BEK-2211 (Mature BDNF Rapid TM ELISA) and BEK-2217 (proBDNF Rapid TM ELISA), for specific use instructions for each substrate application, in particular blood samples. For human blood samples, we suggest the use of BEK-2241 (Mature BDNF/proBDNF Combo kit) . The Mature BDNF ELISA kit consists of a pre-coated mouse monoclonal anti-mature BDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The proBDNF ELISA kit consists of a pre-coated polyclonal anti-proBDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature BDNF or proBDNF present in samples and protein standards. A BDNF and proBDNF positive control (QC sample) is provided to assure consistent assay performance. Both ELISA kits contain high quality protein calibrators. The Mature BDNF ELISA kit employs a recombinant E.coli-derived human mature BDNF standard approved by the World Health Organization (WHO, www.nibsc.org). The amino acid sequence of mature BDNF is identical for human, mouse, rat and a number of other species. This kit therefore is suitable to measure mature BDNF in all these species and uses the same antibodies and antigen. The proBDNF ELISA kit employs a recombinant mammalian, cleavage-resistant human proBDNF standard produced by Biosensis and validated against externally available proBDNF proteins. Due to a high degree of amino acid sequence homology, mouse and rat proBDNF can be quantified and expressed as human proBDNF equivalents. Internal Biosensis validation suggests that the use of the human standard provided in this kit will provide estimates that are identical, or close, to the actual levels of rat and mouse proBDNF present in rodent samples. Note that accurate proBDNF quantification in human serum and citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately. This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
See BEK-2211 & BEK-2217 for specific details
Applications:
ELISA
Application Details:
ELISA. For the quantification of Mature BDNF and proBDNF in Culture Supernatant, Serum, Plasma (Citrate), Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 2 x 96-well pre-coated strip plates per kit (1 x BDNF antibody, 1 x proBDNF antibody coated plate), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Sharma GP et al. (2021) Brain-derived neurotrophic factor promotes immune reconstitution following radiation injury via activation of bone marrow mesenchymal stem cells. PLoS One. 16(10):e0259042 Application: Mouse, culture supernatant. Weaver KR et al. (2021) Neuronal-enriched extracellular vesicles in individuals with IBS: A pilot study of COMT and BDNF. Neurogastroenterol Motil. [Epub ahead of print] Application: Human, plasma, extracellular vesicles.
Specificity:
Mature BDNF ELISA: Human, mouse, rat BDNF and numerous other species. Although the BDNF assay antibodies are raised against the mature domain, the mature BDNF ELISA preferentially detects mature BDNF over full-length proBDNF. proBDNF ELISA: Human, mouse and rat proBDNF. The capture antibody used in the proBDNF ELISA kit binds to epitopes within the pro-domain of proBDNF. Thus, this ELISA detects the full length and potentially truncated form of proBDNF, and does not quantify mature BDNF.
Typical limit of detection (LOD) for mature BDNF is less than 2 pg/mL, and 10 pg/mL for proBDNF determined as 150% of the blank value.
Cross Reactivity:
<b>Cross-reactivity of BDNF isoforms:</b><br><br><b>Mature BDNF ELISA:</b><br>No cross-reactivity is observed for nerve growth factor (NGF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL in assay buffer. The reactivity of full-length proBDNF (0.125 ng/mL - 5 ng/mL) was determined in six independent assays using proBDNF proteins from four different sources (mammalian and bacterial, wild-type and mutated). The average cross-reactivity of proBDNF was found to be 5.3% +/- 0.5% in weight (w/v) concentration, or 12.1% +/- 1.2% in molar concentration (mean +/- SEM).<br><br><b>proBDNF ELISA:</b><br>A cross-reactivity of 2% in weight concentration (0.9% in molar concentration) has been observed for mature BDNF assayed at 25 ng/mL (893 pmol/L) in Assay Diluent A.Due to a high degree of sequence homology, this human proBDNF ELISA kit cross-reacts with the mouse and rat form of proBDNF. Other species have not yet been tested, but cross-reactivity with a wide range of mammalian forms of proBDNF is expected.The antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
The Biosensis NGFR/p75 ECD Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit s a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of human p75 neurotrophin receptor in less than 4 hours in urine only if used as directed. Please refer to the kit protocol for specific use instructions for urine application. This ELISA kit consists of a pre-coated mouse monoclonal anti-p75 ECD capture antibody, a biotinylated mouse monoclonal anti-p75 ECD detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75 ECD present in samples and protein standards. A human p75 ECD positive control (QC sample) is provided to assure consistent assay performance. This NGFR/p75 ECD ELISA kit employs a recombinant human p75 ECD -Fc chimera. While there is a current lack of a commercially available, true human p75 ECD standard, this ELISA kit allows quantification of human p75 ECD as p75 ECD -Fc human equivalents. Please note that the antibodies used in this ELISA do not cross-react with mouse p75 ECD . Note: For research use only. Not for diagnostic and clinical purposes. Receive a 20% discount on the Biosensis Creatinine (Urinary) Colorimetric Assay Kit (CRE-001) if purchased together with this ELISA kit in one transaction. To receive a quote, contact us at sales@biosensis.com .
Background Info:
The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant human p75 (AA 1-250) -Fc chimera, made in 293 cells
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor receptor, extracellular domain (NGFR/p75ECD) in Urine. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The Biosensis NGFR/p75 ECD Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit s a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of human p75 neurotrophin receptor in less than 4 hours in urine only if used as directed. Please refer to the kit protocol for specific use instructions for urine application. This ELISA kit consists of a pre-coated mouse monoclonal anti-p75 ECD capture antibody, a biotinylated mouse monoclonal anti-p75 ECD detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75 ECD present in samples and protein standards. A human p75 ECD positive control (QC sample) is provided to assure consistent assay performance. This NGFR/p75 ECD ELISA kit employs a recombinant human p75 ECD -Fc chimera. While there is a current lack of a commercially available, true human p75 ECD standard, this ELISA kit allows quantification of human p75 ECD as p75 ECD -Fc human equivalents. Please note that the antibodies used in this ELISA do not cross-react with mouse p75 ECD . Note: For research use only. Not for diagnostic and clinical purposes. Receive a 20% discount on the Biosensis Creatinine (Urinary) Colorimetric Assay Kit (CRE-001) if purchased together with this ELISA kit in one transaction. To receive a quote, contact us at sales@biosensis.com .
Background Info:
The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant human p75 (AA 1-250) -Fc chimera, made in 293 cells
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor receptor, extracellular domain (NGFR/p75ECD) in Urine. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The Biosensis proBDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, human serum and EDTA-plasma only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples. This ELISA kit consists of a pre-coated monoclonal anti-proBDNF capture antibody, a biotinylated anti-matureBDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proBDNF present in samples and protein standards. A proBDNF positive control (QC sample) is provided to assure consistent assay performance. This proBDNF ELISA kit employs a recombinant, cleavage-resistant human proBDNF standard produced in mammalian cells by Biosensis and validated against externally available proBDNF proteins. Note that accurate proBDNF quantification in human serum and EDTA-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately . This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Human recombinant proBDNF, mutated to be cleavage-resistant, made in 293F cells
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, pro- (proBDNF) in Culture Supernatant, Serum, Plasma (Citrate), Plasma (EDTA), Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Tsotsoros CE et al. (2022) Pilot Associations between Adverse Childhood Experiences, Executive Function, and Brain-Derived Neurotrophic Factor (BDNF) among Adults with Excess Adiposity Obesities. 2, 276-284. Application: Human, serum. Freidle M et al. (2022) Behavioural and neuroplastic effects of a double-blind randomised controlled balance exercise trial in people with Parkinson's disease. NPJ Parkinsons Dis. 8(1):12. Application: Human, serum. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human, urine. Yi X et al. (2021) Serum mBDNF and ProBDNF Expression Levels as Diagnosis Clue for Early Stage Parkinson's Disease. Front Neurol. 12:680765. Application: Human, serum. Nomura S et al. (2021) Effects of a Tea Cultivar "MK5601" on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice. J Nutr Sci Vitaminol (Tokyo). 67(3):170-179. Application: Mouse, hippocampal RIPA homogenates. Li P et al. (2021) Intermediation of perceived stress between early trauma and plasma M/P ratio levels in obsessive-compulsive disorder patients. J Affect Disord. 285:105-111 Application: Human, plasma. Lai NS et al. (2021) Increased Serum Levels of Brain-Derived Neurotrophic Factor Contribute to Inflammatory Responses in Patients with Rheumatoid Arthritis. Int. J. Mol. Sci. 22(4):1841 Application: Human, serum and culture supernatants. Fukumoto M et al. (2019) Induction of brain-derived neurotrophic factor in enteric glial cells stimulated by interleukin-1? via a c-Jun N-terminal kinase pathway. J. Clin. Biochem. Nutr. [Epub ahead of print]. Application: Human, culture supernatant.
Specificity:
Human proBDNF. The capture antibody used in this ELISA kit binds to an epitope within the pro-domain of proBDNF. Thus, this ELISA detects the full length form of proBDNF and does not quantify mature BDNF. Whether this ELISA kit can detect truncated BDNF is unknown at present. Mature BDNF cross-reactivity was assessed by spiking 28 kDa mature BDNF protein obtained from WHO (www.nibsc.org) into a 1/5 diluted human serum sample at 5 ng/mL, which represents a BDNF concentration level of 25 ng/mL in undiluted, normal human serum. Cross-reactivity of mature BDNF was The assay antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
Typical limit of detection (LOD) for proBDNF is 6 pg/mL determined as 150% of the blank value.
Cross Reactivity:
Mature BDNF cross-reactivity was assessed by spiking 28 kDa mature BDNF protein obtained from WHO (www.nibsc.org) into a 1/5 diluted human serum sample at 5 ng/mL, which represents a BDNF concentration level of 25 ng/mL in undiluted, normal human serum. Cross-reactivity of mature BDNF was < 0.3% (w/v), or < 0.1% in molar concentration.<br><br>The assay antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
The Biosensis proBDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, human serum and EDTA-plasma only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples. This ELISA kit consists of a pre-coated monoclonal anti-proBDNF capture antibody, a biotinylated anti-matureBDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proBDNF present in samples and protein standards. A proBDNF positive control (QC sample) is provided to assure consistent assay performance. This proBDNF ELISA kit employs a recombinant, cleavage-resistant human proBDNF standard produced in mammalian cells by Biosensis and validated against externally available proBDNF proteins. Note that accurate proBDNF quantification in human serum and EDTA-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately . This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Human recombinant proBDNF, mutated to be cleavage-resistant, made in 293F cells
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, pro- (proBDNF) in Culture Supernatant, Serum, Plasma (Citrate), Plasma (EDTA), Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Tsotsoros CE et al. (2022) Pilot Associations between Adverse Childhood Experiences, Executive Function, and Brain-Derived Neurotrophic Factor (BDNF) among Adults with Excess Adiposity Obesities. 2, 276-284. Application: Human, serum. Freidle M et al. (2022) Behavioural and neuroplastic effects of a double-blind randomised controlled balance exercise trial in people with Parkinson's disease. NPJ Parkinsons Dis. 8(1):12. Application: Human, serum. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human, urine. Yi X et al. (2021) Serum mBDNF and ProBDNF Expression Levels as Diagnosis Clue for Early Stage Parkinson's Disease. Front Neurol. 12:680765. Application: Human, serum. Nomura S et al. (2021) Effects of a Tea Cultivar "MK5601" on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice. J Nutr Sci Vitaminol (Tokyo). 67(3):170-179. Application: Mouse, hippocampal RIPA homogenates. Li P et al. (2021) Intermediation of perceived stress between early trauma and plasma M/P ratio levels in obsessive-compulsive disorder patients. J Affect Disord. 285:105-111 Application: Human, plasma. Lai NS et al. (2021) Increased Serum Levels of Brain-Derived Neurotrophic Factor Contribute to Inflammatory Responses in Patients with Rheumatoid Arthritis. Int. J. Mol. Sci. 22(4):1841 Application: Human, serum and culture supernatants. Fukumoto M et al. (2019) Induction of brain-derived neurotrophic factor in enteric glial cells stimulated by interleukin-1? via a c-Jun N-terminal kinase pathway. J. Clin. Biochem. Nutr. [Epub ahead of print]. Application: Human, culture supernatant.
Specificity:
Human proBDNF. The capture antibody used in this ELISA kit binds to an epitope within the pro-domain of proBDNF. Thus, this ELISA detects the full length form of proBDNF and does not quantify mature BDNF. Whether this ELISA kit can detect truncated BDNF is unknown at present. Mature BDNF cross-reactivity was assessed by spiking 28 kDa mature BDNF protein obtained from WHO (www.nibsc.org) into a 1/5 diluted human serum sample at 5 ng/mL, which represents a BDNF concentration level of 25 ng/mL in undiluted, normal human serum. Cross-reactivity of mature BDNF was The assay antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
Typical limit of detection (LOD) for proBDNF is 6 pg/mL determined as 150% of the blank value.
Cross Reactivity:
Mature BDNF cross-reactivity was assessed by spiking 28 kDa mature BDNF protein obtained from WHO (www.nibsc.org) into a 1/5 diluted human serum sample at 5 ng/mL, which represents a BDNF concentration level of 25 ng/mL in undiluted, normal human serum. Cross-reactivity of mature BDNF was < 0.3% (w/v), or < 0.1% in molar concentration.<br><br>The assay antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
The Biosensis Mouse and Rat proNGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rodent full-length proNGF protein in less than 4 hours in cell culture supernatants and cell lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli and consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. This ELISA kit is expected to detect rat proNGF due to high degree of homology (96%) with mouse proNGF based on amino acid sequence, and the ability of this kit in detecting proNGF in rat PC12 cell lysates. In the absence of a true rat proNGF standard, results may be expressed as 'mouse proNGF equivalents'. This ELISA kit shows only 20% reactivity with the human form of proNGF and is therefore not suitable to quantify human proNGF. No cross-reactivity was observed with mature mouse NGF and full-length proBDNF when tested in assay buffer. The antibodies used in this ELISA kit bind epitopes within the pro-domain (capture) and mature domain (detection) of the protein, thus this ELISA assay does not detect the pro-domain peptide. This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
Product Type:
ELISA Assay
Species Reactivity:
Mouse,Rat
Immunogen:
Recombinant mouse, wild-type proNGF protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor, pro- (proNGF) in Culture Supernatant, Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
pro-beta nerve growth factor; proNGF; NGF
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Luu BE et al. (2022) Modulation of diabetic kidney disease markers by an antagonist of p75NTR in streptozotocin-treated mice Gene. [Epub ahead of print]. Application: Mouse kidney RIPA homogenates. Mossa A et al. (2021). Adaptation to partial urethral obstruction in healthy aging LOU rats and the role of nerve growth factor signaling pathway in the bladder. Exp Gerontol. [Epub ahead of print]. Application: Rat urine. Sugimoto J et al. (2021). Fabry disease-associated globotriaosylceramide induces mechanical allodynia via activation of signaling through proNGF p75NTR but not mature NGF TrkA. Eur. J. Pharmacol. 895. Application: Mouse tissue homogenate (RIPA). Mossa AH et al. (2020). Antagonism of proNGF or its receptor p75NTR reverses remodelling and improves bladder function in a mouse model of diabetic voiding dysfunction. Diabetologia. [Epub ahead of print]. Application: Mouse bladder extracts (RIPA). Ryu JC et al. (2018). Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. [Epub ahead of print]. Application: Mouse urine.
Specificity:
Mouse proNGF. Expected to detect rat proNGF due to high degree of sequence homology. Detects human proNGF (about 20% reactivity). Does not cross-react with proBDNF and mature NGF.
Typical limit of detection (LOD) for mouse proNGF is less than 50 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
Reacts with human proNGF (about 20% reactivity). Does not cross-react with proBDNF and mature NGF. Mature NGF spiked into brain homogenate does not interfere with proNGF quantification.
The Biosensis Mouse and Rat proNGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rodent full-length proNGF protein in less than 4 hours in cell culture supernatants and cell lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli and consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. This ELISA kit is expected to detect rat proNGF due to high degree of homology (96%) with mouse proNGF based on amino acid sequence, and the ability of this kit in detecting proNGF in rat PC12 cell lysates. In the absence of a true rat proNGF standard, results may be expressed as 'mouse proNGF equivalents'. This ELISA kit shows only 20% reactivity with the human form of proNGF and is therefore not suitable to quantify human proNGF. No cross-reactivity was observed with mature mouse NGF and full-length proBDNF when tested in assay buffer. The antibodies used in this ELISA kit bind epitopes within the pro-domain (capture) and mature domain (detection) of the protein, thus this ELISA assay does not detect the pro-domain peptide. This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
Product Type:
ELISA Assay
Species Reactivity:
Mouse,Rat
Immunogen:
Recombinant mouse, wild-type proNGF protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor, pro- (proNGF) in Culture Supernatant, Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
pro-beta nerve growth factor; proNGF; NGF
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Luu BE et al. (2022) Modulation of diabetic kidney disease markers by an antagonist of p75NTR in streptozotocin-treated mice Gene. [Epub ahead of print]. Application: Mouse kidney RIPA homogenates. Mossa A et al. (2021). Adaptation to partial urethral obstruction in healthy aging LOU rats and the role of nerve growth factor signaling pathway in the bladder. Exp Gerontol. [Epub ahead of print]. Application: Rat urine. Sugimoto J et al. (2021). Fabry disease-associated globotriaosylceramide induces mechanical allodynia via activation of signaling through proNGF p75NTR but not mature NGF TrkA. Eur. J. Pharmacol. 895. Application: Mouse tissue homogenate (RIPA). Mossa AH et al. (2020). Antagonism of proNGF or its receptor p75NTR reverses remodelling and improves bladder function in a mouse model of diabetic voiding dysfunction. Diabetologia. [Epub ahead of print]. Application: Mouse bladder extracts (RIPA). Ryu JC et al. (2018). Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. [Epub ahead of print]. Application: Mouse urine.
Specificity:
Mouse proNGF. Expected to detect rat proNGF due to high degree of sequence homology. Detects human proNGF (about 20% reactivity). Does not cross-react with proBDNF and mature NGF.
Rabbit anti-Adrenocorticotropic hormone (ACTH) Polyclonal Antibody (Unconjugated), suitable for IHC-Paraffin-embedded.
Background Info:
Adrenocorticotropic hormone (ACTH) is cleaved from the precursor pro-opiomelanocortin (POMC). The hormone is produced and secreted by the pituitary gland and stimulates release of cortisol by adrenal glands.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Pig,Rat
Immunogen:
Porcine Adrenocorticotropic hormone (ACTH) conjugated to BSA
Applications:
IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Corticotropin; ACTH; Pro-opiomelanocortin; POMC;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human; mouse; rat; ACTH is highly conserved so cross-reactivity with other species is expected.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Rabbit anti-Tyrosine Kinase Receptor B (TrkB) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, ELISA.
Background Info:
TrkB is a member of the neurotrophic tyrosine receptor kinase family. It is a membrane-bound receptor and upon neurotrophin binding, it phosphorylates itself as well as MAPK pathways members. TrkB is the receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). It is Involved in the development and/or maintenance of the nervous system. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 4 named isoforms produced by alternative splicing. Additional isoforms seem to exist. TISSUE SPECIFICITY: The different forms are differentially expressed in various cell types. SIMILARITY: Belongs to the Tyr protein kinase family. Insulin receptor subfamily. SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SIMILARITY: Contains 2 LRR (leucine-rich) repeats. SIMILARITY: Contains 1 protein kinase domain. Mutations in the TrkB gene have been associated with obesity and mood disorders.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
Extracellular domain of glycosylated mouse TrkB protein produced in CHO cells was used as the immunogen. As shown for similar antisera, it is anticipated that this antibody will block the TrkB receptor binding of corresponding neurotrophin ligand.
Applications:
ELISA,IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IF (1:1000-1:3000), 1-site ELISA (1:10,000 dilution). A dilution of 1:1000 to 1:3000 o/n is recommended for IF. 4% PFA frozen sections tested. Not yet tested on paraffin embedded tissues. Use triton X-100 permeabilizaiton with frozen sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rudolph M (2020). Mesolimbic GluA1 AMPA Receptor Signaling in Dopaminergic Neurons Plays a Critical Role in the Induction of Cross-Sensitization to Psychostimulants in Response to Social Stress. PhD Thesis. Application: IHC (IF), frozen sections. Species: Rat. Penzo MA et al. (2015). The paraventricular thalamus controls a central amygdala fear circuit. Nature. [Epub ahead of print]. Application: IHC (IF), 4% PFA, frozen sections. Species: Mouse.
Specificity:
Specificity was demonstrated by immunohistochemistry. This antibody was used to stain cryostat sections of the rat peripheral sensory ganglia. Reacts with rat and mouse TrkB. Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Tyrosine Hydroxylase (TH) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. FUNCTION: Plays an important role in the physiology of adrenergic neurons. CATALYTIC ACTIVITY: L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin. COFACTOR: Fe(2+) ion. ENZYME REGULATION: Phosphorylation leads to an increase in the catalytic activity. PATHWAY: Catecholamine biosynthesis; first step. SUBUNIT: Homotetramer. PTM: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation. SIMILARITY: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (SPRFIGRRQSLIEDARK) as part of human Tyrosine Hydroxylase (aa: 32-47) conjugated to KLH
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
<b>Immunohistochemistry (IHC):</b> 0.5-1 ug/mL. This is a superb antibody for detection of tyrosine hydroxylase containing neurons exhibiting an intense labelling with a negligible background. This antiserum has proven extremely useful for staining of catecholaminergic neurons. It stains nicely and intensely dendritic processes and fine nerve terminals.<br><b>Western Blotting (WB):</b> 0.5-2 ug/mL. This antibody demonstrates clear immunoreactivity for TH at 60 kDa in rat PC12 cell lysate and mouse brain homogenate. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
IHC on brain shows a pattern of staining specific for TH containing neurons. This antibody is known to react with rat. Cross reactivity with other species has not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
MAP1A and MAP1B are microtubule-associated protein which mediate the physical interactions between microtubules and components of the cytoskeleton (probably involved in autophagosome formation). MAP1A and MAP1B each consist of a heavy chain subunit and 3 different light chain subunits (LC1, LC2 and LC3). MAP1LC3A is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor form of MAP1LC3A is cleaved by APG4/ATG4B to form the cytosolic form LC3-1. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. MAP1LC3A is most abundant in heart, brain, liver, skeletal muscle and testis but is absent in thymus and peripheral leukocytes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (RSFADRCKEVQQI) corresponding to the N-terminal of human MAP1LC3 A protein conjugated to Blue Carrier Protein has been used as the immunogen. The sequence is homologous with mouse and rat MAP1LC3 A.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunohistochemistry on 4% PFA fixed or 2% PLP fixation, 1:100-1000 primary antibody dilution. IC, Immunocytochemistry: acetone fixed specimens recommended. 1:100-1:1000 primary antibody dilution. Western Blot: R-146-100 requires samples to be denatured ONLY by boiling in SDS solution, not reduced. No signal is achieved using reduced samples. (Samples treated with DTT or Beta mercaptoethanol). 1:500 overnight 4 degrees is recommended for primary antibody dilution in western blots. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
IHC, WB and ELISA confirmed the specificity for MAP1LC3 A. Human, rat. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Autophagy-related protein 10 (ATG10) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
ATG10 plays a role in autophagy. It is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugations and modificatioon of a soluble form of MAPLC3 1A, a homolog of yeast ATG8, to a membrane bound form. It is also able to directly interact either with ATG5 or ATG7.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A peptide (CLKNSQKINKNV) corresponding to the C-terminal of human ATG10 protein has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence. A dilution of 1:100 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy-related protein 10; APG10-like; ATG10
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Ryningen A et al (2012) Inhibition of Mammalian target of rapamycin in human acute myeloid leukemia cells has diverse effects that depend on the environmental in vitro stress. Bone Marrow Res. 2012;2012:329061.
Specificity:
IHC, WB and ELISA confirmed the specificity for ATG10. Human. Other species not yet tested.
Storage:
After reconstitution keep aliquots at minus 20ºC for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Gamma-aminobutyric acid receptor-associated protein (GABARAP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
GABARAP is highly positively charged in its N-terminus and shares sequence homology with MAP1LC3 1A and 1B. This protein clusters neurotransmitter receptors (GABA(A) receptors) by mediating interaction with the cytoskeleton. SUBUNIT: Interacts with GABRG2, TUBA1, ULK1 and NSF. Interacts with beta-tubulin and GPHN. SUBCELLULAR LOCATION: Intracytoplasmic membrane. Cytoskeleton. Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules. TISSUE SPECIFICITY: Heart, brain, placenta, skeletal muscle, kidney and pancreas.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (FEKRRSEGEKIC) corresponding to the N-terminal of human GABARAP protein has been used as the immunogen. The sequence is homologous with mouse and rat form of GABARAP.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
IHC, WB and ELISA confirmed the specificity for GABARAP. Human, rat. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Purification:
Whole serum
Target:
Gamma-aminobutyric acid receptor-associated protein (GABARAP)
FUNCTION: Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. SUBUNIT: Monomer. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ULK1. SUBCELLULAR LOCATION: Golgi apparatus. TISSUE SPECIFICITY: Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine. SIMILARITY: Belongs to the MAP1 LC3 family. ESTIMATED MOLECULAR WEIGHT: 13.667 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (CVESAKIRAKYP) corresponding to the N-terminal of human GABARAP L2 (GABARAPL2) protein has been used as the immunogen. The sequence is homologous with mouse and rat form of GABARAP L2 (GABARAPL2).
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Gamma-aminobutyric acid receptor-associated protein-like 2; GABA(A) receptor-associated protein-like 2; Ganglioside expression factor 2; GEF-2; General protein transport factor p16; MAP1 light chain 3-related protein; GABARAPL2; FLC3A; GEF2;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
IHC, WB and ELISA confirmed the specificity for GABARAP L2 (GABARAPL2). A 14 kDa band, that corresponds to the molecular weight of GABARAPL2, is detected via western blot analysis. Human, rat. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Beclin-1 Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Beclin-1 plays a central role in autophagy. It may also play a role in antiviral host defense. It is ubiquitously expressed and it localises to the cytoplasm, golgi apparatus membrane, and in dendrites and cell bodies of cerbellar Purkinje cells. The unprocessed precursor has a length of 450 amino acids and an estimated molecular weight of 51.89 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
C-terminal of human Beclin-1 conjugated to Blue Carrier Protein.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:100 to 1:2000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Coiled-coil myosin-like BCL2-interacting protein; Protein GT197; APG 6; BECN1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specific for Beclin-1. Human, other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. The enzyme also binds to free monoubiquitin and may prevent its degradation in lysosomes (ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Bovine,Chicken,Horse,Human,Mouse,Pig,Rat
Immunogen:
Recombinant full length human Ubiquitin C Terminal Hydrolase 1 (UCHL1) purified from E. coli.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:1,000 - 1:2,000 is recommended for WB. A dilution of 1:500-1:1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB. This antibody detects ~24 kDa UCHL1 enzyme. Suitable control tissue is rat spinal cord or peripheral nerve homogenate. Hu, Rat, Ms, Bov, Por
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Ubiquitin Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Ubiquitin is a highly conserved 76 amino acid protein with an estimated molecular weight of 8.56 kDa which has a central role in regulated protein degradation. It is a protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Several types of polymeric chains can be formed depending on the lysine used for the assembly. Attachment to proteins as a polymer leads to their degradation by the 26S proteosome; a complex, multicatalytic cytosolic and nuclear protease. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatic structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats of which differ between species and strains. In some species there is a final amino-acid after the last repeat, here in bovine a Cys. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Glutaraldehyde cross-linked ubiquitin.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB. This antibody detects ~8.5 kDa Ubiquitin. Human. Predicted to react with other mammalian tissues.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Peripherin Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Peripherin is a class-III neuronal intermediate filament protein found in certain classes of neuron, most of which are located in the peripheral nervous system.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
Recombinant full length Peripherin protein expressed in and purified from E.coli.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:2,000 - 1:10,000 is recommended for WB. A dilution of 1:1,00-1:2,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Peripherin; Prph; Prph1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Ise H. et al (2017) Elucidation of GlcNAc-binding properties of type III intermediate filament proteins, using GlcNAc-bearing polymers. Genes Cell. 2017 Sep; [Epub ahead of print] Sekerkova G. et al (2008) Espin actin-cytoskeletal proteins are in rat type I spiral ganglion neurons and include splice-isoforms with a functional nuclear localization signal. J Comp Neurol. 2008 Aug 20;509(6):661-76.
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody detects ~57 kDa Peripherin protein and a smaller molecule derived from Peripherin at ~48 kDa. Hu, Rat, Ms, Fel, Rb. Predicted to react with other mammalian tissue.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Microtubule-associated protein 1 light chain 3 gamma (MAP1LC3C) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
FUNCTION: Probably involved in formation of autophagosomal vacuoles (autophagosomes). SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. SUBCELLULAR LOCATION: LC3-I: Cytoplasm. LC3-II: Intracytoplasmic membrane; lipid-anchor. LC3-II binds to the autophagic membranes. TISSUE SPECIFICITY: Most abundant in placenta, lung and ovary. PTM: The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. SIMILARITY: Belongs to the MAP1 LC3 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide (CQEEVAGIRAKF) corresponding to the N-terminal of human MAP1LC3 C conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence deriven from MAP1LC3 C protein in Macaca mulatta (monkey) and Canis familiaris (dog).
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence, WB. A dilution of 1:100 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
IHC and WB confirmed the specificity for MAP1LC3 C . This antibody should recognise MAP1LC3 C only and not the other forms MAP1LC3A and MAP1LC3B. Human, other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Purification:
Whole serum
Target:
Microtubule-associated protein 1 light chain 3 gamma (MAP1LC3C)
Rabbit anti-Neuron specific enolase (NSE) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Enolase is a metalloenzyme that catayzes the reaction between 2-phospho-D-glycerate and phosphoenolpyruvate during glycolysis. Mammalian enolase is composed of 3 subunits; alpha, beta and gamma (Neuron-specific enolase). These subunits can form homodimers or heterodimers. The alpha/gamma heterodimer and the gamma/gamma homodimer are found primarily in neurons.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human Neuron Specific Enolase (NSE) expressed in and purified from E.coli
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:1,000 - 1:2,000 is recommended for WB. A dilution of 1:500 is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rabbit anti-Neurofilament Medium (NF-M) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Bovine,Chicken,Horse,Human,Mouse,Pig,Rat
Immunogen:
A recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:1,000 - 1:5,000 is recommended for WB, ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specifically recognizes the medium neurofilament subunit NF-L in WB. Band appears at ~145 kDa in WB from rodent and ~160 kDa for human and bovine WB. Hu, Rat, Ms, Fel, Chk
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
Recombinant full length human NF-L protein
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:10,000 - 1:20,000 is recommended for WB. A dilution of 1:2,000 - 1:5,000 is recommended for ICC/IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rabbit anti-Neurofilament Heavy, phosphorylated and non-phosphorylated (pNF-H) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human,Other Mammals (Predicted),Rat
Immunogen:
Purified rat NF-H construct containing most of the tandem KSP repeats expressed and purified from E.coli.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunocytochemistry (IC). Suggested dilution for WB of 1:5,000-10,000. Suggested dilution for IC is 1:500-1,000. This antibody stains dendritic and perikaryal neurofilaments particularly well. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody reacts with noth phosphorylated NF-H at approx 200 kDa and non-phosphorylated NF-H at 160 kDa. Rat. Predicted to react with other mammals due to sequence homology.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Purification:
Whole serum
Target:
Neurofilament Heavy, phosphorylated and non-phosphorylated (pNF-H)
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Bovine,Horse,Human,Mouse,Pig,Rat
Immunogen:
Native NF-H purified from bovine spinal cord
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunocytochemistry (IHC). Suggested dilution for WB of 1:5,000-10,000. Suggested dilution for ICC/IHC is 1:1,000-1:5,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Hashiguchi S et al. (2019) "Ataxic phenotype with altered CaV3.1 channel property in a mouse model for spinocerebellar ataxia 42." Neurobiol Dis. Jun 20:104516 [Epub ahead of print]. Application: IHC. Species: Human.
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody reacts with phosphorylated NF-H and is seen as a band of approx 200 kDa. Rat. Predicted to react with other mammals due to sequence homology.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. MMP2 isoform 2 mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways. Catalytic activity of MMP2 causes cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln. (Ref: uniprot.org).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Western Blotting (WB): 1:5,000 - 1:10,000. MMP2 appears as two bands at apparent molecular weights of 66 and 72 kDa. Immunohistochemistry (IHC): 1:500-1:1,000.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
72 kDa gelatinase; Gelatinase A; MMP-2; TBE-1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human. Expected to react with horse, cow, pig, chicken, rat and mouse MMP2.
Storage:
Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Myristoylated alanine rich C kinase substrate (MARCKS) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Myristoylated alanine-rich C-kinase substrate (MARCKS) is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. Ref: SWISSPROT.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant full length MARCKS expressed and purified from E. coli
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:10,000 - 20,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Myristoylated alanine-rich C-kinase substrate; MARCKS; Protein kinase C substrate 80 kDa protein;Marcks; Macs;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB. Does not react with rodent protein.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Purification:
Whole serum
Target:
Myristoylated alanine rich C kinase substrate (MARCKS)
Rabbit anti-Autophagy protein 5 (ATG5) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. FUNCTION: May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. SUBCELLULAR LOCATION: Cytoplasm. Colocalizes with nonmuscle actin. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. INDUCTION: By apoptotic stimuli. PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane. SIMILARITY: Belongs to the ATG5 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to the C-terminal of human ATG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
Flow Cytometry (2 ?g/10^6 cells), IHC, immunofluorescence, WB. Use a dilution of 1:200 to 1:1000 for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy protein 5; APG5-like; APG 5; APG5; Apoptosis-specific protein; ATG5; APG5L; ASP
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Villanueva-Paz M. et al. (2016) Amitriptyline induces mitophagy that precedes apoptosis in human HepG2 cells. Genes Cancer. 2016;7(7-8):260-277 Garrido-Maraver J. et al (2012) Screening of effective pharmacological treatments for MELAS syndrome using yeasts, fibroblasts and cybrids models of the disease Br J Pharmacol. 2012 Jul 2. De la Mata M. et al (2012) Recovery of MERRF fibroblasts and cybrids pathophysiology by Coenzyme Q10 Neurotherapeutics. 2012 Apr;9(2):446-63. Cotan D. et al. (2011) Secondary coenzyme Q10 deficiency triggers mitochondria degradation by mitophagy in MELAS fibroblasts FASEB J. 2011
Specificity:
IHC and wb confirmed the specificity for ATG5. Human, not yet tested in other species.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Alpha-internexin is a ~66 kDa Class IV intermediate filament subunit expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Cat,Human,Mouse,Other Mammals,Rat
Immunogen:
Recombinant alpha-internexin expressed and purified from E. coli
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:10,000 - 1:20,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB. This antibody is specific for the 64-66 kDa alpha-internexin protein. Molecular weight will depend on species. Hu, Rat, Ms, Fel, and other mammals
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Glial Fibrillary Acidic Protein (GFAP) Polyclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
Background Info:
GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Bovine,Horse,Human,Mouse,Pig,Rat
Immunogen:
Recombinant full length human GFAP isotype 1 expressed in and purified from E. coli.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:5,000 is recommended for WB, and 1:1,000 - 1:5,000 for ICC and IHC. A dilution of 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme-linked methods is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Astrocyte; Glial fibrillary acidic protein; GFAP;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Autophagy-related protein 12 (ATG12) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Required for autophagy. SUBUNIT: Conjugated to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: Belongs to the ATG12 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to the N-terminal of human ATG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC, immunofluorescence, WB (1-5 µg/mL working concentration), Flow Cytometry (2?g/10^6 cells). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy-related protein 12; APG12-like; ATG12; APG12; APG12L
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
IHC and WB confirmed the specificity for ATG12. Human, rat
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Coronin- 1A Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Coronins belong to the WD40 or WD family of proteins. Coronins appear to be particularly involved in binding to actin, actin associated proteins, tubulin and phospholipase C and have been implicated in the mechanisms of chemotaxis and phagocytosis. In mammals there are at least five major coronin proteins, named coronins 1 to 5 in one nomenclature. Another nomenclature divides these five proteins in coronins 1a and 1b, 2a, 2b and 2c (see the Human Genone Organization Gene Nomenclature Committee link for this family). The mammalian coronin family members are abundant components of eukaryotic cells and each type has a restricted cell type specific expression pattern. Coronin 1A is found exclusively in hematopoetic lineage cells such as lymphocytes, macrophages and neutrophils. This antibody is therefore an excellent marker of cells of this lineage and can also be used to study the leading edges particularly of neutrophils. Since the only hematopoetic cells found within the central nervous system are microglia, this antibody is also an excellent marker of this important cell type. Microglia are numerically fairly minor components of the nervous system, but microglial activation is seen in response to a wide variety of damage and disease states, including ALS, Alzheimer's disease and responses to brain tumors. Since coronin 1a is a constitutive component of microglia, the coronin 1a antibody can be used to study both quiescent and activated microglia.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
C-terminal peptide of human coronin 1a coupled to KLH
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:2,500-5,000 is recommended for WB. Human Coronin 1A has a predicted length of 461 residues and a MW of 51 kDa. A concentration of 1:500-1:1,000 is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Coronin-1A; Coronin-like protein A; Clipin-A; Coronin-like protein p57; Tryptophan aspartate-containing coat protein; TACO; CORO1A; CORO1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Stephens A.N. et al (2010) Post-translational modifications and protein-specific isoforms in endometriosis revealed by 2D DIGE. J Proteome Res. 2010 May 7;9(5):2438-49. Ahmed Z. et al (2007) Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry. J Histochem Cytochem. 2007 Jul;55(7):687-700.
Specificity:
The specificity of this antibody has been confirmed by WB and ICC against the antigen. Human, Rat, Mouse and Feline. Predicted to react with other mammalian tissue.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-PRKR-like endoplasmic reticulum kinase (phosphorylated and non phosphorylated) (PERK) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
PERK (PKR-like ER kinase) is a single-pass type I ER membrane protein with a stress-sensing luminal domain connected by a transmembrane segment to a cytoplasmic-kinase domain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 50% glycerol
Host Animal:
Rabbit
Species Reactivity:
Mouse
Immunogen:
A recombinant peptide from mouse PERK.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunoprecipitation (IP). A dilution of 1:500 is recommended for WB. A dilution of 30 µL of antibody in a total reaction mixture of 500 µL is recommended for IP. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Feliziani C. et al (2022) Ca2+ signalling system initiated by Endoplasmic reticulum stress stimulates PERK activation Cell Calcium. 2022 [Epub ahead of print] Bollo M. et al (2010) Calcineurin interacts with PERK and dephosphorylates calnexin to relieve ER stress in mammals and frogs PLoS One. 2010 Aug 5;5(8)
Specificity:
This antiserum is known to recognise both phosphorylated and non phosphorylated mouse PERK.
Storage:
Aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Purification:
Whole serum
Target:
PRKR-like endoplasmic reticulum kinase (phosphorylated and non phosphorylated) (PERK)
Rabbit anti-Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
APPL2 is an isoform of APPL1 with 54% identity in their protein sequences. APPL2 has a role in cell proliferation and embryonic development. Recently, it was shown that APPL2 regulates FSH signaling and acts as a negative regulator in adiponectin signaling.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 15% glycerol
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
Mouse APPL2 protein (amino acids: 619-662) conjugated to GST.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunoprecipitation (IP). A dilution between 1:2500 and 1:5000 is recommended for WB. A concentration of 3 µL/reaction is recommended for IP. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
DCC-interacting protein 13-beta; Dip13-beta; Adapter protein containing PH domain; PTB domain and leucine zipper motif 2; APPL2; DIP13B;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum is known to recognise both mouse and human APPL2.
Storage:
Aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Purification:
Whole serum
Target:
Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2)
Rabbit anti-Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
APPL1 (adaptor protein containing PH domain, PTB domain and leucine zipper motif 1) mediates adiponectin signaling in various types of cells. APPL1 is also thought to have a role in insulin signaling.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
The C-terminus of Human APPL1 protein (amino acids: 455-709) conjugated to GST.
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunoprecipitation (IP). A dilution of 1:1,000 is recommended for WB. A concentration of 3 µL/reaction is recommended for IP. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
DCC-interacting protein 13-alpha; Dip13-alpha; Adapter protein containing PH domain; PTB domain and leucine zipper motif 1; APPL1; APPL; DIP13A; KIAA1428;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum is known to recognise both mouse and human APPL1.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Purification:
Whole serum
Target:
Adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1)
Adiponectin Receptors 1 and 2 are membrane receptors for adiponectin, a hormone secreted by adipocytes which regulates energy homeostatis and insulin sensitivity.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 15% glycerol
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
Human Adiponectin Receptor 2 protein (amino acids: 78-219) conjugated to GST.
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting. A dilution of 1:2000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Progestin and adipoQ receptor family member II; ADIPOR2; PAQR2;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum is known to recognise both mouse and human Adiponectin Receptor 2.
Storage:
Aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Adiponectin Receptors 1 and 2 are membrane receptors for adiponectin, a hormone secreted by adipocytes which regulates energy homeostatis and insulin sensitivity.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 15% glycerol
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
The intracellular portion of mouse Adiponectin Receptor 1 protein (amino acids: 4-142) conjugated to GST.
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting. A dilution of 1:2000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Adipor1; AdipoR1; Progestin and adipoQ receptor family member I; ADIPOR1; PAQR1;TESBP1A; CGI-45;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum is known to recognise both mouse and human Adiponectin Receptor 1.
Storage:
Aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Rabbit anti-Myc Tag Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
The Myc tag contains the amino acids Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu (E-Q-K-L-I-S-E-E-D-L) corresponding to amino acids 410-419 of human Myc. This tag is widely used for monitoring expression of recombinant proteins in bacteria, insect and mammalian cells.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS, pH 7.4 with 0.05% sodium azide. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap.
Host Animal:
Rabbit
Species Reactivity:
Species Independent
Immunogen:
Myc epitope tag peptide
Applications:
ELISA,WB
Antibody Isotype:
IgG
Application Details:
ELISA and Western Blotting (WB). Suggested dilutions for ELISA at 1:1,000 and WB 1:500 - 1:1,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Martel, D. et al. (2017) Characterisation of Casein Kinase 1.1 in Leishmania donovani Using the CRISPR Cas9 Toolkit. BioMed Res. Int. 2017 Article ID 4635605. Application: WB
Specificity:
This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Storage:
Maintain at -20°C prior to opening for up to 1 year. The product is also stable at 2-8°C undiluted for several weeks. After opening store at -20°C in undiluted aliquots for up to six months. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Avoid repeated freezing and thawing.
Rabbit anti-Maltose-Binding Protein Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Maltose binding protein (MBP) is encoded by the malE gene of E.coli. MBP is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS, pH 7.4 with 0.05% sodium azide
Host Animal:
Rabbit
Species Reactivity:
Species Independent
Immunogen:
MBP epitope tag recombinant protein.
Applications:
ELISA,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB) and ELISA. Suggested starting dilutions for WB of 1:1,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins.
Storage:
Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
The 6X His tag is a short peptide sequence of 6 histidine residues. Epitopes such as the 6X His tag are often included with the target DNA at the time of cloning to produce fusion proteins containing the tag sequence. This allows anti-epitope tag antibodies such as this one to serve as a universal detection reagent for any recombinant protein containing this tag. Anti-epitope antibodies are a useful alternative to generating antibodies to identify a specific recombinant protein. The 6X His motif is often used as a tag on recombinant proteins to facilitate purification with immobilized metal-affinity chromatography.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS, pH 7.4 with 0.02% sodium azide.
Host Animal:
Rabbit
Species Reactivity:
Species Independent
Immunogen:
The antiserum was produced by immunizing rabbit with synthesized peptide containing 6 Histidine residues.
Applications:
ELISA,ICC,IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
ELISA, WB, IP, IF, IHC. Western Blotting at a suggested concentration of 1:500~1:1000. ELISA at a suggested concentration of 1: 2500. IP 5 µg/sample. IF 5 µg/mL. IHC 5 µg/mL. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
His-tagged fusion proteins
Storage:
Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Rabbit anti-Influenza hemagglutin (HA) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
The Human influenza hemagglutin (HA) tag corresponds to a region (98-106 amino acids) from the HA molecule.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Host Animal:
Rabbit
Species Reactivity:
Species Independent
Immunogen:
The antiserum was produced by immunizing rabbit with synthesized peptide containing the influenza hemagglutinin epitope (YPYDVPDYA).
Applications:
WB
Antibody Isotype:
IgG
Application Details:
Western Blotting at a suggested dilution 1:500~1:1000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Antibody detects HA-tagged proteins (on amino- and carboxy termini) in transfected mammalian cells.
Storage:
Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
Rabbit anti-Contactin-6 Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
The neural adhesion molecule Contactin-6, also known as NB-3, is a contactin/F3 subgroup member of immunoglobulin superfamily. It is expressed exclusively in the nervous system and mainly upregulated at the early postnatal stage during mouse brain development. Employing Northern blot analysis Kamei et al found that amongst different regions of the adult human nervous system cerebellum expressed highest level of NB-3 mRNA. The expression of NB-3 in the cerebellum increases until adulthood. In contrast, the expression in the cerebrum declines to a low level after postnatal day 7. NB-3 like other neural recognition molecules plays a vitally important role in axonal guidance during development, plasticity, and maintenance of synaptic connections in the adult brain. Cui et al recently showed that NB-3 acts as a novel Notch ligand to participate in oligodendrocyte generation. Furthermore, NB-3 triggers nuclear translocation of the Notch intracellular domain and promotes oligodendrogliogenesis from progenitor cells and differentiation of oligodendrocyte precursor cells via Deltex1. In primary oligodendrocytes, NB-3 increases myelin-associated glycoprotein transcripts. Hence, the NB-3/Notch signaling pathway may be worthwhile a closer examination for its potential for the treatment of demyelinating diseases. Human NB-3 shares with rat NB-3 86% identity in nucleotide sequences and 90% identity in amino acid sequences. FUNCTION: Contactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Involved in motor coordination. SUBCELLULAR LOCATION: Cell membrane; lipid-anchor; GPI-anchor. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Expressed in brain. In brain, it is preferentially expressed in the accessory olfactory bulb, layers II/III and V of the cerebral cortex, piriform cortex, anterior thalamic nuclei, locus coeruleus of the pons and mesencephalic trigeminal nucleus and in Purkinje cells of the cerebellum. DEVELOPMENTAL STAGE: Highly expressed after birth, reaching a maximum at the postnatal day 7, and declines thereafter in the cerebrum, whereas it increases in the cerebellum to adulthood.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Rat
Immunogen:
A synthetic peptide (KLHKMSSLSSVGVQILKPSTQF) as part of rat contactin-6 conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1: 5000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neural recognition molecule NB-3; mNB-3
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum stains beautifully the NB3 in DRG, where it nicely stains a subpopulation of neurons and in the trigeminal nerve root, where staining is restricted to the oligodendrocytes. Confirmed to react with rat Contactin-6
Storage:
After reconstitution keep aliquots at minus 20ºC for increased stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. CATALYTIC ACTIVITY: 2 superoxide + 2 H+ = O2 + H2O2. COFACTOR: Binds 1 copper ion per subunit. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. DISEASE: Defects in SOD1 are the cause of familial amyotrophic lateral sclerosis (FALS); also called amyotrophic lateral sclerosis 1 (ALS1 or ALS). ALS is a degenerative disorder of motorneurons in the cortex, brainstem and spinal cord. ALS is characterized by muscular weakness and atrophy beginning in the hands and spreading to the forearms and legs. Muscle fasciculations are commonly visible. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. ALS is sometimes referred to as Lou Gehrig disease after the famous American baseball player who was diagnosed with the disorder. FALS, the familial form of ALS, accounts for about 10% of the cases and is transmitted in an autosomal dominant manner. The mean age at onset of FALS is 45 years. MISCELLANEOUS: Zinc binding promotes dimerization. SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide (ASGEPVV LSGQIT) as part of mouse superoxide dismutase (SOD1) protein (aa: 24-36) conjugated to diphtheria toxoid
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. A dilution of 1:500 to 1:1000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Superoxide dismutase [Cu-Zn]; SOD1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Park KH et al. (2013) Postnatal muscle modification by myogenic factors modulates neuropathology and survival in an ALS mouse model. Nat Commun. 2013;4:2906.
Specificity:
Specificity was confirmed by western blot detecting mouse superoxide dismutase (SOD1). This antiserum is known to react with mouse and rat superoxide dismutase (SOD1) protein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. CATALYTIC ACTIVITY: 2 superoxide + 2 H+ = O2 + H2O2. COFACTOR: Binds 1 copper ion per subunit. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. DISEASE: Defects in SOD1 are the cause of familial amyotrophic lateral sclerosis (FALS); also called amyotrophic lateral sclerosis 1 (ALS1 or ALS). ALS is a degenerative disorder of motorneurons in the cortex, brainstem and spinal cord. ALS is characterized by muscular weakness and atrophy beginning in the hands and spreading to the forearms and legs. Muscle fasciculations are commonly visible. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. ALS is sometimes referred to as Lou Gehrig disease after the famous American baseball player who was diagnosed with the disorder. FALS, the familial form of ALS, accounts for about 10% of the cases and is transmitted in an autosomal dominant manner. The mean age at onset of FALS is 45 years. MISCELLANEOUS: Zinc binding promotes dimerization. SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide (ESNGPVK VWGSIK) as part of human superoxide dismutase (SOD1) protein (aa: 24-36) conjugated to diphtheria toxoid
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. A dilution of 1:500 to 1:1000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Superoxide dismutase [Cu-Zn]; SOD1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity was confirmed by western blot detecting human superoxide dismutase (SOD1). This antiserum is known to react with human superoxide dismutase (SOD1).
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Noxa Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
The Bcl-2 family of proteins which regulate apoptosis share identical sequences called Bcl-2 Homology domains (BH1-4). The BH3 proteins, including BID, NOXA, PUMA, BIK, BIM and BAD are all pro-apoptotic and share sequence identity within the amphipathic alpha-helical BH3 region, which is essential for their apoptotic function. NOXA is highly expressed in adult T-cell leukemia cell line.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse
Immunogen:
A synthetic peptide (CAQLRR IGDKVNLRQK) as part of mouse Noxa (aa: 75-90) conjugated to diphtheria toxoid
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. A dilution of 1:1000 to 1:2000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
PMAIP1; phorbol-12-myristate-13-acetate-induced protein 1; adult T cell leukemia-derived PMA-responsive; Immediate-early-response protein APR; PMA-induced protein 1; Pmaip1; Noxa
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Western blot analysis of cells infected with Noxa adenoviruses and BAF indicates a high level of specificity for this antiserum. This antiserum cross-reacts with mouse. Not yet tested in other species.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Noxa Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
The Bcl-2 family of proteins which regulate apoptosis share identical sequences called Bcl-2 Homology domains (BH1-4). The BH3 proteins, including BID, NOXA, PUMA, BIK, BIM and BAD are all pro-apoptotic and share sequence identity within the amphipathic alpha-helical BH3 region, which is essential for their apoptotic function. NOXA is highly expressed in adult T-cell leukemia cell line.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse
Immunogen:
A synthetic peptide (MPGRKARRNA PVNPTR) as part of mouse Noxa (aa: 1-16) conjugated to diphtheria toxoid
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. A dilution of 1:1000 to 1:2000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
PMAIP1; phorbol-12-myristate-13-acetate-induced protein 1; adult T cell leukemia-derived PMA-responsive; Immediate-early-response protein APR; PMA-induced protein 1; Pmaip1; Noxa
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Western blot analysis of cells infected with Noxa adenoviruses and BAF indicates a high level of specificity for this antiserum. This antiserum cross-reacts with mouse. Not yet tested in other species.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Tyrosine Kinase Receptor B (TrkB) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
FUNCTION: Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures (By similarity). Binds APS. Interacts with SQSTM1. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. Additional isoforms seem to exist. TISSUE SPECIFICITY: The different forms are differentially expressed in various cell types. Isoform T2 is primarily expressed in neurons. PTM: Ligand-mediated auto-phosphorylation. SIMILARITY: Belongs to the Tyr protein kinase family. Insulin receptor subfamily. SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SIMILARITY: Contains 2 LRR (leucine-rich) repeats. SIMILARITY: Contains 1 protein kinase domain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide (AFPRLEPNSIDPEN(C)) as part of rat TrkB protein (aa: 54-67) conjugated to KLH
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1:3000 is recommended for this application. For WB, a 1:500 to 1:1000 dilution is recommended. The sequence of the immunogen and the conjugate are identical to the one used by Yan et al (see the Refs) and the antiserum appears to have similar characteristics. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Warren PM et al. (2018) "Rapid and robust restoration of breathing long after spinal cord injury." Nat Commun. 2018 Nov. PMID: 30482901 9(1):4843. Application: IHC/IF. Species: Rat, spinal cord sections. Wang T et al. (2016) "Flux of signalling endosomes undergoing axonal retrograde transport is encoded by presynaptic activity and TrkB." Nat Commun. 2016 Sep. PMID: 27687129 30(7):12976. Application: IF & structured illumination microscopy (SIM). Species: Rat, Mouse; fixed 4% PFA with 4% sucrose Matusica D et al. (2016) Inhibition of motor neuron death in vitro and in vivo by a p75 neurotrophin receptor intracellular domain fragment. J Cell Sci. 2016 Feb 1;129(3):517-30. Application: WB. Brock JH et al. (2010) Local and remote growth factor effects after primate spinal cord injury. J Neurosci. 2010 Jul 21;30(29):9728-37. Gruber H. et al. (2008) Brain-derived neurotrophic factor and its receptor in the human and the sand rat intervertebral disc. Arthritis Res Ther. 2008; 10(4): R82.
Specificity:
Sequence was chosen to avoid cross reactivity with TrkA and TrkC. This antiserum recognises human, rat and mouse TrkB. Cross reactivity with other species has not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: This is a specific receptor for parathyroid hormone. The activity of this receptor is mediated by g proteins which activate adenylyl cyclase. PTHR2 may be responsible for PTH effects in a number of physiological systems. It may play a significant role in pancreatic function. PTHR2 presence in neurons indicates that it may function as a neurotransmitter receptor. SUBUNIT: Binds to TIPF39/TIP39. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. TISSUE SPECIFICITY: Abundantly expressed in brain, arterial and cardiac endothelium. Found as well in sperm, in the head of the epididymis. Lower expression is found in vascular smooth muscle, exocrine pancreas, testis and placenta. SIMILARITY: Belongs to the G-protein coupled receptor 2 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Rat
Immunogen:
A synthetic peptide (RQIDSHVTLPGYVWSSSEQDC) of rat Parathyroid hormone receptor protein (aa: 481-501) conjugated to KLH has been used as the immunogen.
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. A dilution of 1:500 to 1:1000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity has been shown by western blot. A band of 63 kDa corresponding to the theoretical molecular weight of Parathyroid hormone receptor is easily detectable by WB. Absorption of the antiserum with the immunising peptide abolished the binding of the antibody to the target hence detectability of the band. This antibody is known to react with rat Parathyroid hormone receptor.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Tyrosine Hydroxylase (TH) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. FUNCTION: Plays an important role in the physiology of adrenergic neurons. CATALYTIC ACTIVITY: L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin. COFACTOR: Fe(2+) ion. ENZYME REGULATION: Phosphorylation leads to an increase in the catalytic activity. PATHWAY: Catecholamine biosynthesis; first step. SUBUNIT: Homotetramer. PTM: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation. SIMILARITY: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Guinea Pig,Mouse,Rat
Immunogen:
A synthetic peptide (PRFIGRRQSLIEDARK) as part of human Tyrosine Hydroxylase (63-78) conjugated to KLH has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from TH protein in rat (31-47).
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
<b>Immunohistochemistry (IHC):</b> 1:2,000 to 1:5,000, dilutions of up to 1:100,000 have been reported. This is a superb antibody for detection of tyrosine hydroxylase (TH) containing neurons exhibiting an intense labelling with a negligible background. This antiserum has proven extremely useful for staining of catecholaminergic neurons. It stains nicely and intensely dendritic processes and fine nerve terminals. We recommend mouse or rat brain containing catecholaminergic neurons as a positive control for this antibody, for example brain stem or striatum. <br><b>Western blotting (WB)</b>: 1:100 to 1:500. Antibody has been tested on RIPA-extracted PC12 cell lysate and shown to be specific for TH (~60 kDa). Tissue homogenates show a higher level of non-specific binding and presence of uncharacterized bands. Affinity-purified anti-TH antibody (R-148-50) is recommended for tissue homogenates. <br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Pierre S.R., Lemmens M.A., Figueiredo-Pereira M.E. (2009) Subchronic infusion of the product of inflammation prostaglandin J2 models sporadic Parkinson's disease in mice J Neuroinflammation. Jul 25;6:18 Takeoka A. et al (2010) Noradrenergic innervation of the rat spinal cord caudal to a complete spinal cord transection: effects of olfactory ensheathing glia J Exp Neurol. 2010 Mar;222(1):59-69. Brown R.E. et al (2008) Characterization of GABAergic neurons in rapid-eye-movement sleep controlling regions of the brainstem reticular formation in GAD67-green fluorescent protein knock-in mice. Eur J Neurosci. 2008 Jan;27(2):352-63. Bisem NJ et al (2012) Mapping of FGF1 in the Medulla Oblongata of Macaca fascicularis. Acta Histochem Cytochem. 2012 Dec 26;45(6):325-34.
Specificity:
IHC on brain shows a pattern of staining specific for TH containing neurons. This antibody is known to react with rat, mouse and guinea pig. Cross reactivity with other species has not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Synphilin-1 (Sph1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Synuclein alpha interacting protein (Synphilin-1) contains several protein-protein interaction domains and interacts with alpha synuclein in neurons. Mutations of SNCAIP have been linked to Parkinson disease. The amino acid sequence of synphilin-1 shares a high level of identity with its human counterpart, particularly in regions containing ankyrin-like motifs and the coiled-coil domain. Expression pattern of synphilin-1 in tissues is similar in both mouse and human. Synphilin-1 has an important role in the formation of aggregates and cytotoxicity in Parkinson disease and also Dorfin may be involved in the pathogenic process by ubiquitylation of synphilin-1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (CASKGKNKAA) as part of human synphilin-1a conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1: 1000 to 1:2000 is recommended for immunohistochemistry and 1:500-1:1000 for western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A high level of specificity has been shown for this antiserum by IHC on human brain. This antiserum is known to react with rat and human synphilin-1a.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Synphilin-1 (Sph1) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Synuclein alpha interacting protein (Synphilin-1) contains several protein-protein interaction domains and interacts with alpha synuclein in neurons. Mutations of SNCAIP have been linked to Parkinson disease. The amino acid sequence of synphilin-1 shares a high level of identity with its human counterpart, particularly in regions containing ankyrin-like motifs and the coiled-coil domain. Expression pattern of synphilin-1 in tissues is similar in both mouse and human. Synphilin-1 has an important role in the formation of aggregates and cytotoxicity in Parkinson disease and also Dorfin may be involved in the pathogenic process by ubiquitylation of synphilin-1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (SLELNGEKDKDKGRTLQRT) as part of human synphilin-1a conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1: 1000 to 1:2000 is recommended for this application. This antiserum will stain Lewy bodies in Dorsal raphe nucleus of Parkinson's diseased human brain. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A high level of specificity has been shown for this antiserum by IHC on human brain. This antiserum is known to react with rat and human synphilin-1a.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Ubiquitin-like protein which binds to a wide range of target proteins. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. SUBUNIT: Covalently attached to a number of proteins such as PmL, RANGAP1, HIPK2, SP100, p53, p73alpha, MDM2, JUN and DNMT3B. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, PIAS1, EXOSC9, TDG, RAD51 and RAD52. SUBCELLULAR LOCATION: Nucleus; nuclear membrane. Nucleus; nucleoplasm; nuclear speckle. Cytoplasm. SIMILARITY: Belongs to the ubiquitin family. SMT3 subfamily. SIMILARITY: Contains 1 ubiquitin-like domain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (AKPSTEDLGDKKEGEY) as part of human SUMO-1 peptide (aa: 6-21) conjugated to diphtheria toxoid has been used as the immunogen. This antigen is homologous with SUMO-1 of rat.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:1000 to 1:2000 is recommended for immunohistochemistry and 1:2000 to 1:4000 for western blot. Cell lysate from Hela and NIH-3T3 cell lysates may be used as a positive control, and for IHC, lung carcinoma may be used. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Small ubiquitin-related modifier 1; Ubiquitin-like protein SMT3C; SMT3 homolog 3; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein UBL1; GAP-modifying protein 1; GMP1; Sentrin; SUMO1; SMT3C; SMT3H3; UBL1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum recognises human SUMO-1 and not ubiquitin. This antiserum is known to cross react with rat and human SUMO-1.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Parkinson disease protein 2 (Parkin) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include SYT11, CCNE1, GPR37, STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP and SEPT5. May play a more general role in the ubiquitin proteasomal pathway by participating in the removal and/or detoxification of abnormally folded or damaged protein. Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of PARK2. May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Regulates cyclin E during neuronal apoptosis. May represent a tumor suppressor gene. SUBCELLULAR LOCATION: Cytoplasm. Co-localizes with STY11 in neutrites. Co-localizes with SNCAIP in brainstem Lewy bodies. TISSUE SPECIFICITY: Highly expressed in the brain including the substantia nigra. Expressed in heart, testis and skeletal muscle. Expression is down-regulated or absent in tumor biopsies, and absent in the brain of PARK2 patients. Overexpression protects dopamine neurons from kainate-mediated apoptosis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (NSLIKELHHFRILGEEQ) as part of human Parkin conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:1000 is recommended for immunohistochemistry and 1:2000 for western blot. Nice staining is achieved in neuronal and cytoplasmic granules sections treated with citrate buffer for antigen retrieval. Few inclusions are stained but these were not positively identified as Lewy bodies. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Ubiquitin E3 ligase PRKN; Parkinson juvenile disease protein 2; Parkinson disease protein 2; PARK2; PRKN
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Song Y. J. C. et al (2009). Degeneration in different parkinsonian syndromes relates to astrocyte type and astrocyte protein expression. J. Neuropathol. Exp. Neurol. Oct 2009;68(10):1073-1083 Huang Y. et al (2008). LRRK2 and parkin immunoreactivity in multiple system atrophy inclusions. Acta Neuropathol. 2008 Dec;116(6):639-46.
Specificity:
This antiserum is known to be highly specific for Parkin shown by IHC and WB. This antibody is known to react with rat and human Parkin.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Parkinson disease protein 2 (Parkin) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA.
Background Info:
FUNCTION: Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include SYT11, CCNE1, GPR37, STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP and SEPT5. May play a more general role in the ubiquitin proteasomal pathway by participating in the removal and/or detoxification of abnormally folded or damaged protein. Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of PARK2. May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Regulates cyclin E during neuronal apoptosis. May represent a tumor suppressor gene. SUBCELLULAR LOCATION: Cytoplasm. Co-localizes with STY11 in neutrites. Co-localizes with SNCAIP in brainstem Lewy bodies. TISSUE SPECIFICITY: Highly expressed in the brain including the substantia nigra. Expressed in heart, testis and skeletal muscle. Expression is down-regulated or absent in tumor biopsies, and absent in the brain of PARK2 patients. Overexpression protects dopamine neurons from kainate-mediated apoptosis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Guinea Pig,Human,Rat
Immunogen:
A synthetic peptide (RILGEEQYNRYQQYGAEEC) as part of human Parkin conjugated to diphtheria toxoid has been used as the immunogen.
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB, immunoblot, 1-site ELISA. A dilution of 1:500 to 1:2000 is recommended for these applications. This antiserum stains trigeminal motor neurons in rat brain stem. A 50 kDa band was identified in rat brain extract using western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Ubiquitin E3 ligase PRKN; Parkinson juvenile disease protein 2; Parkinson disease protein 2; PARK2; PRKN
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Francelin C et al (2021) BACE1 Inhibition Increases Susceptibility to Oxidative Stress by Promoting Mitochondrial Damage. Antioxidants (Basel). 10(10):1539. E. Rubio de la Torre et al (2009) Combined kinase inhibition modulates parkin inactivation. Hum Mol Genet. 2009 Mar 1;18(5):809-23. D'Agata V. et al (2009) Parkin expression profile in dopamine d3 receptor knock-out mice brains. Neurochem Res. 2009 Feb;34(2):327-32. Tamo W. et al (2007) Parkin is expressed in vascular endothelial cells. Neurosci Lett. 2007 Jun 4;419(3):199-201. Trimmer P.A. et al (2004) Parkinson's disease transgenic mitochondrial cybrids generate Lewy inclusion bodies. J Neurochem. 2004 Feb;88(4):800-12. Denison S.R. et al (2004) Alterations in the common fragile site gene Parkin in ovarian and other cancers. Oncogene. 2003 Nov 13;22(51):8370-8. Pawlyk A.C. et al (2003) Novel monoclonal antibodies demonstrate biochemical variation of brain parkin with age. J Biol Chem. 2003 Nov 28;278(48):48120-8. Horowitz J.M. et al (2001) Spatial distribution, cellular integration and stage development of Parkin protein in Xenopus brain. Brain Res Dev Brain Res. 2001 Jan 31;126(1):31-41.
Specificity:
This antiserum is known to specifically recognise Parkin shown by IHC and WB. This antibody is known to react with Parkin of guinea pig and rat.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Autophagy-related protein 12 (ATG12) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Required for autophagy. SUBUNIT: Conjugated to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: Belongs to the ATG12 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to the N-terminal of human ATG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy-related protein 12; APG12-like; APG 12; ATG12; APG12; APG12L
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
IHC and WB confirmed the specificity for ATG12. Human, rat
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Autophagy protein 5 (ATG5) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. FUNCTION: May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. SUBCELLULAR LOCATION: Cytoplasm. Colocalizes with nonmuscle actin. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. INDUCTION: By apoptotic stimuli. PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane. SIMILARITY: Belongs to the ATG5 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to the C-terminal of human ATG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B has been used to orchestrate/boost the immune response.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy protein 5; APG5-like; APG 5; Apoptosis-specific protein; APG5; ATG5; APG5L; ASP
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
E.N. Wilson et al (2011) A Switch Between Cytoprotective and Cytotoxic Autophagy in the Radiosensitization of Breast Tumor Cells by Chloroquine and Vitamin D. Horm Cancer. 2011 Sep 2. Bristol ML et al (2012) Dual functions of autophagy in the response of breast tumor cells to radiation: cytoprotective autophagy with radiation alone and cytotoxic autophagy in radiosensitization by vitamin D 3. Autophagy. 2012 May 1;8(5):739-53. Guido C et al (2012) Metabolic reprogramming of cancer-associated fibroblasts by TGF-? drives tumor growth: connecting TGF-_ signaling with "Warburg-like" cancer metabolism and L-lactate production. Cell Cycle. 2012 Aug 15;11(16):3019-35.
Specificity:
IHC and WB confirmed the specificity for ATG5. Human, not yet tested in other species.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
DBH is an oxireductase belonging to the copper type II ascorbate-dependent monooxygenase family. DBH exists as a homotetramer composed of two non-covalently bound disulfide-linked dimers. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It binds 2 copper ions and 1 PQQ per subunit . Depending on the presence of a signal peptide, DBH can exist in both soluble and membrane-bound forms.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Bovine,Guinea Pig,Human,Rat
Immunogen:
Native enzyme prepared from Bovine adrenal glands. Purity was greater than 95% by PAGE.
Applications:
IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC (frozen), Western Blot, radioimmunoassay and inhibition of enzymatic activity. Recommended to be used at a dilution of 1:500 to 1: 2000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Bowolaksono A. (2012) Molecular Regulation of Noradrenaline in Bovine Corpus Luteum. HAYATI Journal of Biosciences June 2012 Vol. 19 No. 2, p 81-87 Blanco-Centurion C. et al (2004) Effects of hypocretin2-saporin and antidopamine-beta-hydroxylase-saporin neurotoxic lesions of the dorsolateral pons on sleep and muscle tone. Eur J Neurosci. 2004 May;19(10):2741-52. Ohshita N. et al (2004) Characteristics of the trigeminal depressor response in cats. J Neurosci Res. 2004 Jun 15;76(6):891-901.
Specificity:
This antiserum has been extensively characterised in several laboratories. It reacts with a single band on western blot of bovine adrenal homogenates and stains only cells known to contain DBH, such as sympathetic neurons, adrenal medullary cells and central adrenergic neurons. Staining is abolished by preincubation with the enzyme. This antibody is known to react with bovine, human, guinea pig and rat DBH.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Rabbit anti-Myelin basic protein (MBP) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of approximately 80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP). MBP is an 'intrinsically unstructured' protein with a high proportion (approximately 75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system. A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational. Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic. The message for myelin basic protein is selectively translocated to the ends of the cell processes. Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model. FUNCTION: Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity). SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Myelin membrane; peripheral membrane protein; cytoplasmic side. Cytoplasmic side of myelin. TISSUE SPECIFICITY: Found in both the central and the peripheral nervous system.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Guinea Pig,Human,Rat
Immunogen:
A synthetic peptide (YG SLPQKSQRSQ DENPVV, aa: 68-86) as part of guinea pig MBP protein conjugated to KLH
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1:4000 is recommended. Immunostaining for MBP of abnormal appearing oligodendrocytic process and cell bodies in demyelinating areas. This antibody recognises only areas of myelin degeneration when tested in injured spinal cord and lesioned sciatic nerves. It also stains discrete white matter in the brain of multiple system atrophy. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Myelin Basic Protein
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum recognizes MBP in demyelinated nerve tissues. Immunohistochemical analysis of lesioned rat spinal cord indictaes a high level of specificity for this antiserum. This antiserum reacts with human and rat MBP.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Contactin-6 Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
The neural adhesion molecule Contactin-6, also known as NB-3, is a contactin/F3 subgroup member of immunoglobulin superfamily. It is expressed exclusively in the nervous system and mainly upregulated at the early postnatal stage during mouse brain development. Employing Northern blot analysis Kamei et al found that amongst different regions of the adult human nervous system cerebellum expressed highest level of NB-3 mRNA. The expression of NB-3 in the cerebellum increases until adulthood. In contrast, the expression in the cerebrum declines to a low level after postnatal day 7. NB-3 like other neural recognition molecules plays a vitally important role in axonal guidance during development, plasticity, and maintenance of synaptic connections in the adult brain. Cui et al recently showed that NB-3 acts as a novel Notch ligand to participate in oligodendrocyte generation. Furthermore, NB-3 triggers nuclear translocation of the Notch intracellular domain and promotes oligodendrogliogenesis from progenitor cells and differentiation of oligodendrocyte precursor cells via Deltex1. In primary oligodendrocytes, NB-3 increases myelin-associated glycoprotein transcripts. Hence, the NB-3/Notch signaling pathway may be worthwhile a closer examination for its potential for the treatment of demyelinating diseases. Human NB-3 shares with rat NB-3 86% identity in nucleotide sequences and 90% identity in amino acid sequences. FUNCTION: Contactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Involved in motor coordination. SUBCELLULAR LOCATION: Cell membrane; lipid-anchor; GPI-anchor. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Expressed in brain. In brain, it is preferentially expressed in the accessory olfactory bulb, layers II/III and V of the cerebral cortex, piriform cortex, anterior thalamic nuclei, locus coeruleus of the pons and mesencephalic trigeminal nucleus and in Purkinje cells of the cerebellum.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide (GYPSPHYRWKQ) as part of mouse contactin-6 conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1: 4000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neural recognition molecule NB-3; mNB-3
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antiserum stains beautifully the NB3 in DRG, where it nicely stains a subpopulation of neurons and in the trigeminal nerve root, where staining is restricted to the oligodendrocytes. Confirmed to react with rat Contactin-6 and expected to recognise human and mouse Contactin-6 as well.
Storage:
After reconstitution keep aliquots at -20°C for increased stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Rabbit anti-Nociceptin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, ELISA.
Background Info:
FUNCTION: Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Expressed predominantly in the spinal cord and brain, being more abundant in the hypothalamus and striatum. Also found in small amounts in ovary. PTM: Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin. PTM: The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing. SIMILARITY: Belongs to the opioid neuropeptide precursor family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Guinea Pig,Rat
Immunogen:
A synthetic peptide (C-TG ARKSARKLAN Q) as part of rat Nociceptin peptide (aa: 139-151) conjugated to diphtheria toxoid
Applications:
ELISA,IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC, 1-site ELISA. A dilution of 1: 1000 to 1: 3000 is recommended for both applications. This antibody may also be used for staining of nerve fibres in guinea pig myenteric plexus. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
There are no synonyms for this peptide; however the precursor protein contains: Neuropeptide 1; Nociceptin (Orphanin FQ; PPNOC; ORL1 receptor agonist); Neuropeptide 2
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been tested by the use of different peptides including enkephalin, dynorphin and endorphin for absorption in immunihistochemistry. This antibody is known to cross react with guinea pig and rat.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Orexin-A Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
FUNCTION: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. SUBCELLULAR LOCATION: Endoplasmic reticulum; rough endoplasmic reticulum. Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses. SIMILARITY: Belongs to the orexin family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Bovine,Rat
Immunogen:
A synthetic peptide (CRLYELLHGAGNHAAGILTL) as part of Bovine Orexin A (aa: 14-33) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. This is a superb antiserum for immunohistochemistry on Orexin A containing neurons exhibiting intense labelling of neurons with very low back ground. A dilution of 1:1000 to 1:2000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Orexin-A; Hypocretin-1; Hcrt1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Kruger J.L. et al (2010) Cellular location and major terminal networks of the orexinergic system in the brains of five microchiropteran species. J Chem Neuroanat. 2010 Nov;40(3):256-62. Gaykema R.P. et al (2009) Lipopolysaccharide challenge-induced suppression of Fos in hypothalamic orexin neurons: their potential role in sickness behavior. Brain Behav Immun. 2009 Oct;23(7):926-30. Lee H.S. et al (2005) Retrograde study of hypocretin-1 (orexin-A) projections to subdivisions of the dorsal raphe nucleus in the rat. Brain Res. 2005 Oct 12;1059(1):35-45. Yao S.T. et al (2005) Water deprivation increases the expression of neuronal nitric oxide synthase (nNOS) but not orexin-A in the lateral hypothalamic area of the rat. J Comp Neurol. 2005 Sep 19;490(2):180-93.
Specificity:
The specificity for this antiserum has been confirmed by immunohistochemistry on rat brain and the results reflect the current literature. This antibody is known to react with rat Orexin A.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Neurokinin-3 Receptor (NK-3R) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
FUNCTION: This is a receptor for the tachykinin neuropeptide neuromedin K (neurokinin B). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. PTM: The anchoring of this receptor to the plasma membrane is probably mediated by the palmitoylation of a cysteine residue. MISCELLANEOUS: The rank order of affinity of this receptor to tachykinins is: neuromedin K > substance K > substance P. SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Rat
Immunogen:
A synthetic peptide (ASTTSSF ISSPYTSVDE YS) corresponding to the absolute C-terminal of rat NK-3 receptor protein (aa: 434-452) conjugated to KLH
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. A dilution of 1:500 to 1:2000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neuromedin K receptor; NKR; Neurokinin B receptor; NK-3 receptor; NK-3R; Tachykinin receptor 3; Tacr3; Tac3r
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been shown by western blot using rat brain homogenate. A band of 66 kDa, the theoretical MW of NK-3R, could be easily detected. This antiserum is know to cross react with rat NK-3 R.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Primate,Rat
Immunogen:
Recombinant human NT4
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA (1 site), Western Blot, ICC, inhibition of biological activity in vitro/in vivo. Recommended to be used at a concentration of 1-10 µg/mL for immunohistochemistry, ELISA, ICC and Western blot and inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). Note that the concentration of NT4 is generally low in most tissues nevertheless, neonatal testes of rat can be used as a good positive control. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by dot blot. Known to react with NT4 from rat and human, mouse and monkey.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Primate,Rat
Immunogen:
Recombinant human NT4
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA (1 site), Western Blot, inhibition of biological activity in vitro/in vivo. Recommended to be used at a dilution of 1:500 to 1:2000 for immunohistochemistry, ELISA and Western blot. 1:10 to 1:50 for inhibition of biological activity in vitro. Use neat for in vivo studies at 5-10 µL/g body weight. Note that the concentration of NT4 is generally low in most tissues nevertheless, neonatal testes of rat can be used as a good positive control. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by 1-site ELISA. Known to react with NT4 from rat and human, mouse and monkey.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
CNTF is a survival promoting factor for different types of neurons in vitro and in vivo. The essential structural features for the biological function of human CNTF were investigated by Thier, M. et al. They showed that deletion of 14 N-terminal and 18 C-terminal amino acids significantly increased bioactivity compared to wild-type CNTF. FUNCTION: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Nervous system. PHARMACEUTICAL: CNTF is being tested under the name Axokine by Regeneron Pharmaceuticals for treatment of human motor neuron diseases, such as amyotrophic lateral sclerosis (ALS). As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. SIMILARITY: Belongs to the CNTF family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human CNTF
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. A dilution of 1:500 to 1: 4000 is recommended for western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Ciliary neurotrophic factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody specifically detects CNTF shown by western blot. This antiserum to known to react with rat, mouse and human CNTF protein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
FUNCTION: Seems to promotes the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS with no preservatives.
Host Animal:
Rabbit
Species Reactivity:
Chicken,Human,Rat
Immunogen:
Recombinant human NT3
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A cross reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Seems to promotes the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Chicken,Human,Rat
Immunogen:
Recombinant human NT3
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A dilution of 1:500 to 1:1000 is recommended for IHC, ELISA and western blot. For inhibition of biological activity: 1:10-50 for in vitro, 2-10 µL/g body weight for in vivo. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A cross reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Seems to promote the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Chicken,Human,Rat
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human (aa: 139-149), mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A dilution of 1:500 to 1:2000 is recommended for IHC, western blot. For inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Seems to promotes the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Chicken,Human,Rat
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human (aa: 139-149), mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Feron F et al (2008) Neurotrophin expression in the adult olfactory epithelium. Brain Res. 1196:13-21 Application: IHC ; Species: Rat
Specificity:
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.2-7.6 without preservatives
Host Animal:
Rabbit
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antibody was tested on cultured sensory neurons supported by 100 ng/mL of purified mouse beta NGF. Be advised that 2 µg/mL will neutralize 100 ng/mL of mouse NGF. The higher 10 µg/mL is only recommended if the concentration of NGF being used is higher than 100 ng/mL such as the 200 or 500ng/mL that is occasionally used in some culture systems. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Laurina Z. et al (2009) Growth factors/cytokines/defensins and apoptosis in periodontal pathologies. Stomatologija. 2009;11(2):48-54. Lee H.W. et al (2007) Expression of nerve growth factor is upregulated in the rat thymic epithelial cells during thymus regeneration following acute thymic involution. Regul Pept. 2007 Jun 7;141(1-3):86-95
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
Storage:
Store lyophilized antibody at 2-8ºC. After reconstitution keep aliquots at -20°C to -80ºC for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Rabbit anti-Tumor necrosis factor receptor superfamily member 19 (TROY) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
FUNCTION: Can mediate activation of c-Jun and NF-kappa-B. May promote caspase-independent cell death. Isoform 2 and isoform 3 may act as decoy receptors. SUBUNIT: Associates with TRAF1, TRAF2, TRAF3 and TRAF5. SUBCELLULAR LOCATION: Isoform 1, isoform 3, isoform 4: Cell membrane; single-pass type I membrane protein (Probable). Isoform 2: Secreted protein (Probable). ALTERNATIVE PRODUCTS: 4 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Highly expressed in adult brain, and in embryos from day 11-17, but not earlier. Detected in embryonic brain and epithelium, and at lower levels in adult heart, lung and liver. In neonatal mice, mainly in hair follicles and neuron-like cells in the cerebellum, but not in the skin epidermis. Isoform 3 was found in embryonic day 17.5 skin but not in brain and liver. SIMILARITY: Contains 3 TNFR-Cys repeats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide (CRPHRF KEDWGFQK) as part of mouse TROY protein (aa: 75-88) conjugated to the immunogenic protein Blue Carrier Protein
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. Recommended to be used at a dilution of 1:500 to 1:2000 for immunohistochemistry. This antiserum has not yet been tested for western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Tumor necrosis factor receptor superfamily member 19; TNFRSF19; Toxicity and JNK inducer; TRADE
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity for TROY was confirmed by IHC. This antiserum is known to react with rat TROY. Reactivity with other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Purification:
Whole serum
Target:
Tumor necrosis factor receptor superfamily member 19 (TROY)
Rabbit anti-Huntingtin-associated protein 1 (HAP-1) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
FUNCTION: Associates specifically with huntingtin. This binding is enhanced by an expanded polyglutamine repeat. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: In the brain, especially in the olfactory bulb and in the brain stem. No detectable expression in peripheral tissues such as lung, testis, spleen, and small intestine. SIMILARITY: Contains 1 HAP1 N-terminal domain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Rat
Immunogen:
Recombinant rat HAP-1
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:500 to 1:3000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Huntingtin-associated protein 1; HAP1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity for HAP-1 was confirmed by IHC. This antiserum is known to react with rat HAP-1. Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. DOMAIN: Death domain is responsible for interaction with RANBP9. PTM: N- and O-glycosylated. PTM: O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. PTM: Phosphorylated on serine residues. SIMILARITY: Contains 1 death domain. SIMILARITY: Contains 4 TNFR-Cys repeats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
Extra cellular domain of human p75NTR
Applications:
ICC,IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence. Recommended to be used at a dilution of 1:500 to 1:2000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
IHC shows specific staining for p75NTR. This antibody is known to react to rat p75NTR.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human BDNF
Applications:
IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA (1 site), Western Blot, inhibition of biological activity in vitro/in vivo. Recommended to be used at a dilution of 1:1000 for immunohistochemistry, ELISA and Western blot. 1:10 to 1:50 for inhibition of biological activity in vitro. Use neat for in vivo studies at 5-10 uL/g body weight. This antiserum stains cell bodies and some nerve terminals in the dorsal horn of the rat spinal cord, however, does not stain finest nerve terminals. <br><br>Western Blotting: Antibody does detect BDNF forms in tissue lysates but there are multiple bands present, many of which are uncharacterized. The antibody detects 14 kDa (mature BDNF), 32 kDa (proBDNF) and a 18 kDa BDNF isoform (see blot examples). In cell lysates, only 18 kDa and 32 kDa BDNF are detected. The reason for these differences has not been characterized. Alternative antibodies for Western Blotting are: R-017-500 (IgG-purified form of R-088-100 for tissue homogenate analysis); R-1707-100 (cell lysates and tissue homogenates), R-083-100/R-066-500 (cell lysates, tissue homogenates and human serum); M-1744-50/100 (human serum and tissue homogenates).<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
L.Y. Chen et al (2010) Learning induces neurotrophin signaling at hippocampal synapses Proc Natl Acad Sci USA. Apr 13;107(15):7030-5 Soderquist R.G. et al (2009) PEGylation of brain-derived neurotrophic factor for preserved biological activity and enhanced spinal cord distribution J Biomed Mater Res A. 2009 Dec;91(3):719-29. Tang S. et al (2010) Immunolocalization of pro- and mature-brain derived neurotrophic factor (BDNF) and receptor TrkB in the human brainstem and hippocampus. Brain Res. Oct 1;1354:1-14. Sadri-Vakili G. et al (2010) Cocaine-induced chromatin remodeling increases brain-derived neurotrophic factor transcription in the rat medial prefrontal cortex, which alters the reinforcing efficacy of cocaine. J Neurosci. 2010 Sep 1;30(35):11735-44. Maldonado M.A. et al (2008) Motor skill training, but not voluntary exercise, improves skilled reaching after unilateral ischemic lesions of the sensorimotor cortex in rats. Neurorehabil Neural Repair. 2008 May-Jun;22(3):250-61. Nakajima H. et al (2007) Rescue of rat anterior horn neurons after spinal cord injury by retrograde transfection of adenovirus vector carrying brain-derived neurotrophic factor gene. J Neurotrauma. 2007 Apr;24(4):703-12. Zhang H.T. et al (2007) Immunohistochemical distribution of NGF, BDNF, NT-3, and NT-4 in adult rhesus monkey brains. J Histochem Cytochem. 2007 Jan;55(1):1-19. Carrasco M.A. et al (2007) Regulation of glycinergic and GABAergic synaptogenesis by brain-derived neurotrophic factor in developing spinal neurons. Neuroscience. 2007 Mar 16;145(2):484-94. Zhang H.T. et al (2008) Temporal changes in the level of neurotrophins in the spinal cord and associated precentral gyrus following spinal hemisection in adult Rhesus monkeys J Chem Neuroanat. 2008 Dec;36(3-4):138-43.
Specificity:
Less than 0.1% cross-reactivity against NGF, NT3 and NT4/5 by dot blot. Known to react with BDNF from rat, mouse and human. Expected to react with BDNF from other species due to amino acid sequence homology.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide (C-ELLDEDQKVRPNEE) as a part of human BDNF precursor protein (aa: 69-82) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:1000 to 1:5000 is recommended for both applications. ICC: 1:500 to 1:2000, antibody works on 4% formaldehyde fixed cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Used in western blot, this antiserum detects a 35 kDa band corresponding to the molecular weight of proBDNF. No cross reactivity with other proneurotrophins was detected. This antiserum is known to react with human, mouse and rat proBDNF and also expected to recognise other mammalian proBDNF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A dilution of 1:1000-1:5000 is recommended for IHC, western blot and immunoblot; 1:15000 for ELISA; for inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Mulhall J.P. et al (2008) J Sex Med. May;5(5):1126-36.
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF bot not bovine NGF.
Storage:
Store lyophilized antibody at 2-8ºC. After reconstitution keep aliquots at -20°C to -80ºC for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst primates.
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
<b>Western Blotting:</b> This antibody detects multiple BDNF isoforms (14 kDa mature BDNF, 18 kDa isoform, 28 kDa BDNF dimer/truncated BDNF, 32 kDa proBDNF monomer) depending on sample application (human serum, cell lysate, tissue homogenate). Antibody also detects BDNF under non-reducing conditions (McLean NA, 2014).<br><br><b>ELISA:</b> Detection only, 1:1000-1:5000 recommended.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
McLean NA, Popescu BF, Gordon T, Zochodne DW, Verge VM. (2014) "Delayed nerve stimulation promotes axon-protective neurofilament phosphorylation, accelerates immune cell clearance and enhances remyelination in vivo in focally demyelinated nerves." PLoS One. 2014 Oct 13;9(10):e110174 Application: WB , non-reducing, Species: Rat Cysneiros R.M. et al (2010) Qualitative analysis of hippocampal plastic changes in rats with epilepsy supplemented with oral omega-3 fatty acids Epilepsy Behav. 2010 Jan;17(1):33-8. Ooe N. et al (2009) Dynamic regulation of bHLH-PAS-type transcription factor NXF gene expression and neurotrophin dependent induction of the transcriptional control activity Biochem Biophys Res Commun. 2009 Jan 23;378(4):761-5.
Specificity:
Less than 0.1% cross reactivity with mouse NGF, recombinant human NT3 and NT4/5 has been recorded by dot blot analysis. This antiserum is known to recognise rat, mouse and human BDNF, and is expected to react with BDNF from other species due to amino acid sequence homology.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. <br />FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. <br />DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human GDNF
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB and dot blot. Recommended to be used at a dilution of 1:1000 to 1:3000 for Western blot. The predicted molecular weight of the GDNF monomer is 11.6 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
No cross reactivity with NTN has been observed in Western Blot analysis. This antibody is known to react with human, mouse and rat GDNF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Vanilloid receptor-like protein 1 (VRL-1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
TISSUE SPECIFICITY: Ubiquitously expressed. Expressed in dorsal root ganglia, trigeminal ganglia, spinal chord (Lissauer's tract, dorsal horn and dorsal columns) (at protein level). PTM: N-glycosylated. PTM: Phosphorylated by PKA. SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (C-KNSASEEDHLPLQVLQSP) of rat VRL-1 protein (aa: 744-761) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, Immunofluorescence, Western blot. Recommended to be used at a dilution of 1: 1000 to 1: 2000 for immunohistochemistry and Western blot, for Immunofluorescence at a dilution of 1:50 to 1: 200 in free-floating sections or paraffin embedded sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
osm-9-like TRP channel 2; OTRPC2
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Immunohistochemical analysis in rat dorsal root ganglia and spinal cord indicates a high level of specificity for this antiserum. Specificity was also shown by Western blot. This antibody is known to react with rat and human VRL-1.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent.
Rabbit anti-Vanilloid receptor-like protein 1 (VRL-1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
TISSUE SPECIFICITY: Ubiquitously expressed. Expressed in dorsal root ganglia, trigeminal ganglia, spinal chord (Lissauer's tract, dorsal horn and dorsal columns) (at protein level). PTM: N-glycosylated. PTM: Phosphorylated by PKA. SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4, containing 0.02% sodium azide as preservative.
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (C-KNSASEEDHLPLQVLQSP) as part of rat VRL-1 protein (aa: 744-761) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC, Immunofluorescence, Western blot and Immunoblot. Recommended to be used at a concentration of 0.5-1 µg for these applications. This antiserum works superbly for immunohistochemistry on free-floating or paraffin embedded sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
osm-9-like TRP channel 2; OTRPC2
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Immunohistochemical analysis in rat dorsal root ganglia and spinal cord indicates a high level of specificity for this antiserum. Specificity was also shown by Western blot. This antibody is known to react with rat and human VRL-1.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent.
Rabbit anti-Capsaicin receptor (TrpV1) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Responses evoked by low pH and heat, and capsaicin can be antagonized by capsazepine. SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (PSESTSHRWRGPA) of human capsaicin receptor protein (aa: 608-621) conjugated to KLH has been used as the antigen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. Use at 1:1000 to 1:2000 dilution. This antibody has not been tested in other applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Jabin Fagelskiold A et al (2012) Insulin-secreting INS-1E cells express functional TRPV1 channels. Islets. 2012 Jan 1;4(1).
Specificity:
Specificity was confirmed by IHC using frozen sections of rat dorsal root ganglia (DRG) and spinal cord. Due to sequence homology, similar staining is predicted in human DRG and spinal cord. Human, rat. Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Alpha-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. FUNCTION: May be involved in the regulation of dopamine release and transport. Soluble protein, normally localized primarily at the presynaptic region of axons, which can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase 3 activation. TISSUE SPECIFICITY: Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.SUBUNIT: Soluble monomer which can form filamentous aggregates. Interacts with UCHL1. Interacts with phospholipase D and histones. SUBCELLULAR LOCATION: Cytoplasm. Membrane. Nucleus. Note=Membrane-bound in dopaminergic neurons. Also found in the nucleus. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. Additional isoforms seem to exist.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide (SEEGYQDYEPEA) corresponding to the C-terminal of human alpha synuclein protein (aa 129-140) conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from alpha synuclein protein in monkey and pig.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:500 to 1:3000 is recommended for both applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP; SNCA; PARK1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Tinsley R.B. et al (2010) Sensitive and specific detection of alpha-synuclein in human plasma J Neurosci Res. 2010 Sep;88(12):2693-700.
Specificity:
Immunohistochemical and western blot analysis of human brain indicates a high level of specificity for this antiserum. This antibody is known to react with human, mouse and rat alpha synuclein. Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Adenosine triphosphate (ATP)- ase (ATPase) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein (By similarity). SIMILARITY: Belongs to the cation transport ATPase (P-type) family. Type V subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide (C-ELHRQEEAKQVLRYY) as part of mouse ATP13A2 protein (aa: 147-161) conjugated to KLH
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:500 to 1:2000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Immunohistochemistry shows specific staining for ATPase. This antiserum is known to react with rat ATPase. Other species have not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Adenosine triphosphate (ATP)- ase (ATPase) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein (By similarity). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. SIMILARITY: Belongs to the cation transport ATPase (P-type) family. Type V subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (C-DDVHRSRHGLSLQDQ) as part of human ATP13A2 protein (aa: 195-209) conjugated to KLH
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:500 to 1:2000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Immunohistochemistry shows specific staining for ATPase. This antiserum is known to react with rat ATPase. Other species have not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Amyloid-beta precursor protein (APP) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
FUNCTION: Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1/Tip60 and inhibit Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. May be involved in copper homeostasis/oxidative stress through copper ion reduction. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. FUNCTION: Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. Rat and mouse beta-amyloid peptides bind only weakly transient metals and have little reducing activity due to substitutions of transient metal chelating residues. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation (By similarity). FUNCTION: The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis. SUBUNIT: Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, MAPK8IP1, SHC1, Numb and Dab1. Binding to Dab1 inhibits its serine phosphorylation. Also interacts with GPCR-like protein BPP, FPRL1, APPBP1, IB1, KNS2 (via its TPR domains), APPBP2 (via BaSS) and DDB1. In vitro, it binds MAPT via the MT-binding domains. Associates with microtubules in the presence of ATP and in a kinesin-dependent manner. Interacts, through a C-terminal domain, with GNAO1. Amyloid beta-42 binds CHRNA7 in hippocampal neurons. Beta-amyloid associates with HADH2. TISSUE SPECIFICITY: different isoforms in different tissues: kidney. brain. liver. hippocampus, substania nigra pars compacta and cerebellum. In the cerebellum, all the isoforms are abundantly expressed in Purkinje cells.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide (HMNVQNGKWESDPSGTKTC, aa: 44-62) as part of mouse APP isoform A conjugated to the immunogenic protein Blue Carrier Protein
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. Recommended to be used at a dilution of 1:500 to 1:3000 for immunohistochemistry. This antiserum has not yet been tested for western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Amyloid beta A4 protein; ABPP; Alzheimer disease amyloid protein homolog; Amyloidogenic glycoprotein; AG
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity for APP was confirmed by IHC. This antiserum is known to react with rat APP. Reactivity with other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Amyloid-beta precursor protein (APP) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
FUNCTION: Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1/Tip60 and inhibit Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. May be involved in copper homeostasis/oxidative stress through copper ion reduction. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. FUNCTION: Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. Rat and mouse beta-amyloid peptides bind only weakly transient metals and have little reducing activity due to substitutions of transient metal chelating residues. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation (By similarity). FUNCTION: The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis. SUBUNIT: Binds, via its C-terminus, to the PID domain of several cytoplasmic proteins, including APBB family members, the APBA family, MAPK8IP1, SHC1, Numb and Dab1. Binding to Dab1 inhibits its serine phosphorylation. Also interacts with GPCR-like protein BPP, FPRL1, APPBP1, IB1, KNS2 (via its TPR domains), APPBP2 (via BaSS) and DDB1. In vitro, it binds MAPT via the MT-binding domains. Associates with microtubules in the presence of ATP and in a kinesin-dependent manner. Interacts, through a C-terminal domain, with GNAO1. Amyloid beta-42 binds CHRNA7 in hippocampal neurons. Beta-amyloid associates with HADH2. TISSUE SPECIFICITY: different isoforms in different tissues: kidney. brain. liver. hippocampus, substania nigra pars compacta and cerebellum. In the cerebellum, all the isoforms are abundantly expressed in Purkinje cells.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
Synthetic peptides (C-ETHLHW HTVAKET, aa: 145-157; C-HAH FQKAKERLEA KHRER, aa: 388-405; C-KKKQYTS IHHGVVE, aa: 724-737) as parts of human APP isoform A conjugated to KLH
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. Recommended to be used at a dilution of 1:500 to 1:3000 for immunohistochemistry. This antiserum has not yet been tested for western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity for APP was confirmed by IHC. This antiserum is known to react with rat APP. Reactivity with other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Receptor for the glial cell line-derived neurotrophic factor, artemin. Mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase (By similarity). SUBCELLULAR LOCATION: Cell membrane; lipid-anchor; GPI-anchor (By similarity). SIMILARITY: Belongs to the GDNFR family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide (MGLSWSPRPPL) as part of mouse GFR alpha-3 protein (aa: 1-11) conjugated to KLH
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:500 to 1:1000 is recommended for both applications. This antibody stains nicely large neurons in the rat DRG. Specifically, if you would like to perform WB using rat brain homogenate detecting GFR alpha-3, this is the antibody to be used. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
GDNF family receptor alpha-3; GFR-alpha-3
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Western blot analysis indicates a high level of specificity to GFR alpha-3 for this antiserum. This antibody is known to react with rat GFR alpha-2.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
FUNCTION: Receptor for neurturin. Mediates the NRTN-induced autophosphorylation and activation of the RET receptor. Also able to mediate GDNF signaling through the RET tyrosine kinase receptor. SUBCELLULAR LOCATION: Cell membrane; lipid-anchor; GPI-anchor (By similarity). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (PRVEKTPSLPDDLSD) as a part of human GFR alpha-2 protein (aa: 376-390) conjugated to KLH.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:500 to 1:1000 is recommended for both applications. This antibody stains beautifully the large neurons in the rat DRG. This antibody also is ideal for WB analysis of rat DRG and brain homogenate. The optimal dilution should be determined by the end user, but a dilution of 1 in 500 is recommended for initial testing. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Western blot analysis indicates a high level of specificity to GFR alpha-2 for this antiserum. This antibody is known to react with rat GFR alpha-2 and from IHC analysis, there appears to be no cross-reactivity with other members of the GFR family.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer, and Parkinson disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain.<br />FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst mammalian species.
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
<b>Western Blotting:</b> A concentration of 1-10 µg/mL is recommended for this application. In Western Blotting, this antibody detects multiple BDNF isoforms (14 kDa mature BDNF, 18 kDa isoform, 28 kDa BDNF dimer/truncated BDNF, 32 kDa proBDNF monomer) depending on sample application (human serum, cell lysate, tissue homogenate).<br><br><b>IHC:</b> Antibody works well in immunohistochemistry with the proper fixation, pretreatments and dilution. Formal fixed, paraffin embedded tissue is not recommend. Recommended fixation is Zamboni fixative or light 4% PFA fixation on fixed, frozen tissue. Recommended dilution is 1-10 µg/mL for immunohistochemistry at 4 degrees centigrade for 2-48 hours. <b>ELISA:</b> 1-10 µg/mL capture/detection.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Feron F et al (2008) Neurotrophin expression in the adult olfactory epithelium. Brain Res. 1196:13-21 Application: IHC ; Species: Rat
Specificity:
Less than 0.1% cross reactivity with mouse NGF, recombinant human NT3 and NT4/5 has been recorded by dot blot analysis. This antiserum is known to recognise rat, human and human BDNF, and is expected to react with BDNF from other species due to amino acid sequence homology.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Pan-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Detects human alpha-, beta-, and gamma synuclein proteins. A family of homologous proteins known as alpha-, beta-, and gamma-synuclein are abundantly expressed in brain, especially in the presynaptic terminal of neurons. Although the precise function of these proteins remains unknown, alpha-synuclein has been implicated in synaptic plasticity associated with avian song learning as well as in the pathogenesis of Parkinson's disease (PD), dementia with LBs (DLB), some forms of Alzheimer's disease (AD), and multiple system atrophy (MSA).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (AKEGVVAAAEKTKQGV) as a consensus part of human alpha-, beta-, and gamma synuclein proteins conjugated to diphteria toxoid has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB and immunoblot. A dilution of 1:1000 is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Ulusoy A. et al (2010) Eur J Neurosci. 2010 Aug;32(3):409-22 Naesstrom T. et al (2010) The lipid peroxidation products 4-oxo-2-nonenal and 4-hydroxy-2-nonenal promote the formation of _-synuclein oligomers with distinct biochemical, morphological, and functional properties. Free Radic Biol Med. 2010 Dec 1. Eslamboli A. et al (2007) Long-term consequences of human alpha-synuclein overexpression in the primate ventral midbrain. Brain. 2007 Mar;130(Pt 3):799-815. Mukaetova-Ladinska E.B. et al (2008) Alpha- and gamma-synuclein proteins are present in cerebrospinal fluid and are increased in aged subjects with neurodegenerative and vascular changes. Dement Geriatr Cogn Disord. 2008;26(1):32-42.
Specificity:
Overlap specific immunohistochemical staining of alpha-, beta- and gamma synucleins This antiserum recognises human and rat alpha-, beta- and gamma synucleins.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Gamma-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases. May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway. SUBUNIT: May be a centrosome-associated protein. SUBCELLULAR LOCATION: Cytoplasm; perinuclear region. Centrosome. Spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle. TISSUE SPECIFICITY: Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung. PTM: Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases. DISEASE: Brain iron accumulation type 1 (NBIA1, also called Hallervorden-Spatz syndrome), a rare neuroaxonal dystrophy, is histologically characterized by axonal spheroids, iron deposition, Lewy body (LB)-like intraneuronal inclusions, glial inclusions and neurofibrillary tangles. SNCG is found in spheroids but not in inclusions. SIMILARITY: Belongs to the synuclein family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (EKEEVAEEAQSGGD) as part of human gamma synuclein protein (aa: 114-127) conjugated to diptheria toxid has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:500 to 1:1000 is recommended for both applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Immunohistochemical/western blot analysis indicate a high level of specificity for this antiserum for gamma synuclein. This antiserum is known to react with human and rat gamma synuclein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Beta-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Beta-synuclein is a non-amyloid component of senile plaques found in Alzheimer disease. It could act as a regulator of SNCA aggregation. It protects nerurons from staurosporine and 6 hydroxy dopamine -stimulated capspase activation in a p53-dependent manner. It localises to the cytoplasm and it is predominantly expressed in the brain where it is most concentrated in presynaptic nerve terminals. This protein is phosphorylated. This protein is also associated with the disease Brain iron accumulation type 1 (NBIA1).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (IEPLMEPEGSYEDPPQE) of human beta synuclein protein (aa: 108-125) conjugated to diptheria toxid has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB , immunoblot. A dilution of 1:500 to 1:2000 is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
SNCB
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Israeli E. and Sharon R. (2009) Beta-synuclein occurs in vivo in lipid-associated oligomers and forms hetero-oligomers with alpha-synuclein J Neurochem. 2009 Jan;108(2):465-74
Specificity:
Less than 0.1% cross reactivity to human alpha synuclein This antiserum is known to react with human and rat beta synuclein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Capsaicin receptor (TrpV1) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
TISSUE SPECIFICITY: Predominantly expressed in trigeminal and dorsal root sensory ganglia. Isoform 1 and isoform 3 are also expressed in brain and peripheral blood mononuclear cells.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse (Predicted),Rat
Immunogen:
A synthetic peptide (YFSHLKEYVAS) of human capsaicin receptor protein (aa: 531-541) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. Use at 1:1000 to 1:2000 dilution. This antibody has not been tested in other applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rehman R et al (2013) TRPV1 inhibition attenuates IL-13 mediated asthma features in mice by reducing airway epithelial injury. Int Immunopharmacol. 2013 Mar;15(3):597-605.
Specificity:
Immunohistochemical analysis of rat DRG indicates a superb degree of specificity for this serum. This antibody is known to react with rat Capsaicin and due to sequence homology is expected to react with mouse Capsaicin . Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent.
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Guinea Pig,Mouse,Pig,Rabbit,Rat
Immunogen:
A synthetic peptide (GLFSSYRLPGHTQDTLVAQKSS) as a part of porcine ChAT protein (aa: 167-188) conjugated to KLH
Applications:
ELISA,ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
Mixed
Application Details:
Immunohistochemistry/cytochemistry on 4% PFA or formalin fixed frozen sections; paraffin sections can be more difficult and require more extensive antigen recovery methods. 1:400-1:2000 depending up detection and incubation times<br>ELISA: direct antigen ELISA<br>This antiserum will superbly stain both cell bodies and nerve terminal, and works particularly well in enteric and peripheral neurons. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Beig MI, Dampney BW and Carrive P (2014) Both ox1r and ox2r orexin receptors contribute to the cardiovascular and locomotor components of the novelty stress response in the rat. Neuropharmacology September 16 [Epub ahead of print] Application: IH ; Species: Rat Ellis KM, O'Carroll DC, Lewis MD, Rychkov GY, Koblar SA (2014) Neurogenic potential of dental pulp stem cells isolated from murine incisors. Stem Cell Res Ther. 2014 Feb 27;5(1):30. Application: IF ; Species: Mouse Leong WK, Klaric TS, Lin Y, Lewis MD, Koblar SA (2013) Upregulation of the neuronal Per-Arnt-Sim domain protein 4 (Npas4) in the rat corticolimbic system following focal cerebral ischemia. Eur J Neurosci. 2013 Jun;37(11):1875-84 Application: IH ; Species: Rat Li S et al (2011) The expression and localization of Prune2 mRNA in the central nervous system. Neurosci Lett. Oct 10;503(3):208-14.
Specificity:
This antiserum stains cholinergic neurons in guinea-pig and rabbit. This antiserum is known to react with ChAT of origin guinea-pig, mouse, rat and rabbit.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Saporin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA.
Background Info:
Saporin is a ribosome-inactivating protein (RIP) of type I. This monomeric RNA N-glycosidase purified from seeds of the plant Saponaria officinalis also known as Soapwort, is capable of specific depurination of eukaryotic ribosomes thus arresting protein synthesis. No ligand has been identified in saporin hence its inability to transverse the cell membrane. Due to its toxicity and stability of the structure, saporin has proven extremely useful for construction of immunotoxins. The expected molecular weight of the purified saporin is 29.5 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Plant
Immunogen:
Saporin, whole molecule
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, Immunofluorescence, ELISA, Western Blot. A dilution of 1:200 to 1: 2000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Saponaria officinalis; Common soapwort
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed to react with purified saporin. No cross-reactivity with other molecules has been reported.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human BDNF
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA (1 site), Western Blot, immunoblot, inhibition of biological activity in vitro/in vivo. Recommended to be used at an amount of 1-10 µg/mL for immunohistochemistry, Western blot or immunoblot, 0.1 µg/mL for ELISA and for inhibition of biological activity in vitro 1-10 µg/mL. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum stains cell bodies and some nerve terminals in the dorsal horn of the rat spinal cord, however, does not stain finest nerve terminals. Western blot: 1-10 µg/mL. Tissue homogenate is a recommended sample application for Western Blotting. The antibody detects 14 kDa (mature BDNF), 32 kDa (proBDNF) and a 18 kDa BDNF isoform, however numerous other non-characterized bands may also be present. In cell lysates, only 18 kDa and 32 kDa BDNF are detected. Alternative antibodies for Western Blotting are: R-1707-100 (cell lysates and tissue homogenates), R-083-100/R-066-500 (cell lysates, tissue homogenates and human serum); M-1744-50/100 (human serum and tissue homogenates).<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Serra, M.P. et al. (2022) Anti-Inflammatory Effect of Beta-Caryophyllene Mediated by the Involvement of TRPV1, BDNF and trkB in the Rat Cerebral Cortex after Hypoperfusion/Reperfusion Int. J. Mol. Sci. Mar; 23(7),3633 Lin J.C-Y. and Rosenthal A. (2011) Methods for treating obesity by administering a trkB antagonist US Patent US 7935342 B2 Counts S.E. and Mufson E.J. (2010) Noradrenaline activation of neurotrophic pathways protects against neuronal amyloid toxicity J Neurochem. 2010 May;113(3):649-60. Unsain N. et al (2009) Brain-derived neurotrophic factor facilitates TrkB down-regulation and neuronal injury after status epilepticus in the rat hippocampus J Neurochem. 2009 Oct;111(2):428-40 Salehi Z., Mashayekhi F. (2009) Brain-derived neurotrophic factor concentrations in the cerebrospinal fluid of patients with Parkinson's disease J Clin Neurosci. Jan;16(1):90-3.
Specificity:
Less than 0.1% cross-reactivity against NGF, recombinant NT3 and NT4 by dot blot. This antiserum is known to react with BDNF from rat, mouse and human. Expected to react with BDNF of other species due to amino acid sequence homology.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
PE-1832 is A2 beta casein protein that is purified in its native form from bovine A2/A2 milk to >95% purity. The milk is collected from commercial cows certified free of disease and BSE by the manufacture of the milk. IMPORTANT: This product may give different OD readings as compared to the protein calibrator contained in our A2 Casein ELISA Kit due to its formulation. Thus it is NOT recommended for use as a standard or QC marker in the A2 ELISA assays.
Background Info:
The protein named Casein is the largest group of proteins in mammalian milk, making up about > 80% of total protein content. Different mammals have different amounts of caseins in their milks and not all casein proteins are the same.<br><br>According to published literature, (PMID: 15453478), multiple forms of casein proteins exist in cow's milk, with beta-casein being one of the most predominant forms. Beta casein in domestic cows at lesat, has two alleles or isoforms. Beta A1 and Beta A2.<br><br>A1 beta-casein is found in milk from breeds of cows that originated in northern Europe. These breeds include Holstein, Friesian, Ayrshire, and British Shorthorn.<br><br>A2 beta-casein. is found in milk breeds that originated in the Channel Islands US and southern France. These include Guernsey, Jersey, Charolais, and Limousin cows (PMID: 15867940, PMID: 15453478).<br><br>Traditionally, store bought regular milk contains both A1 and A2 beta-casein proteins, but pure A2 milk can be obtained that originates only from A2 cows.<br><br>Some studies suggest that there could be some sort of a link between the A1 beta-casein and immunological dieseases such as type-1 diabetes and others and work is underway to understand how beta casein, both A1 and A2 may or may not contribute to human illness as well a health.
Product Type:
Protein
Format:
Liquid. Protein is provided as 50 µL aliquot at 0.4 mg/mL in TBS, pH 8.5, containing protease inhibitor. Does not contain preservatives. Use sterile methods when handling.
Applications:
WB
Application Details:
Western blotting (100 ng or less) as a standard, not recommended for ELISA. Other applications not yet tested.
Alternative Names:
Beta Casein; CSN2
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Protein is shipped on ice packs. Upon receipt, divide into aliquots and store product long-term at -20°C. Store working aliquots short-term at 2-8°C as undiluted liquid for one week. AVOID MULTIPLE FREEZE THAWS
PE-1831 is A1 beta casein protein that is in its native form and purified from bovine A1/A1 commercial cow's milk collected from cows that are certified BSE and disease free by the manufacture of the milk. IMPORTANT: This product may give different OD readings as compared to the protein calibrator contained in our A1 Casein ELISA Kit due to its formulation. Thus it is NOT recommended for use as a standard or QC marker in the A1 ELISA assays.
Background Info:
The protein named Casein is the largest group of proteins in mammalian milk, making up about > 80% of total protein content. Different mammals have different amounts of caseins in their milks and not all casein proteins are the same.<br><br>According to published literature, (PMID: 15453478), multiple forms of casein proteins exist in cow's milk, with beta-casein being one of the most predominant forms. Beta casein in domestic cows at lesat, has two alleles or isoforms. Beta A1 and Beta A2.<br><br>A1 beta-casein is found in milk from breeds of cows that originated in northern Europe. These breeds include Holstein, Friesian, Ayrshire, and British Shorthorn.<br><br>A2 beta-casein. is found in milk breeds that originated in the Channel Islands US and southern France. These include Guernsey, Jersey, Charolais, and Limousin cows (PMID: 15867940, PMID: 15453478).<br><br>Traditionally, store bought regular milk contains both A1 and A2 beta-casein proteins, but pure A2 milk can be obtained that originates only from A2 cows.<br><br>Some studies suggest that there could be some sort of a link between the A1 beta-casein and immunological dieseases such as type-1 diabetes and others and work is underway to understand how beta casein, both A1 and A2 may or may not contribute to human illness as well a health.
Product Type:
Protein
Format:
Liquid. Protein is provided as 50 µL aliquot at 0.4 mg/mL in TBS, pH 8.5, containing protease inhibitor. Does not contain preservatives. Use sterile methods when handling.
Applications:
WB
Application Details:
Western blotting (100 ng or less) as a standard, not recommended for ELISA. Other applications not yet tested.
Alternative Names:
Beta Casein; CSN2
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Protein is shipped on ice packs. Upon receipt, divide into aliquots and store product long-term at -20°C. Store working aliquots short-term at 2-8°C as undiluted liquid for one week. AVOID MULTIPLE FREEZE THAWS
Purification:
The product is >95% A1 beta casein based on SDS-PAGE gel. Affinity-purified, native protein
This peptide may be used to block binding activity of antibody to ChAT (# R-043-100 and # R-1621-100).
Product Type:
Peptide
Format:
Lyophilized.
Applications:
Block
Application Details:
Control peptide. This peptide may be used to block binding activity of antibody to ChAT (# R-043-100 and # R-1621-100). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Store lyophilized peptide dry at -20°C to -80°C. Store reconstituted peptide at 2-8°C for maximum of 2 days to avoid degradation. Aliquot the reconstituted peptide and store at -20°C to -80°C; avoid repetitive freeze-thaw cycles.
A proprietary preparation of human amyloid beta peptide (amino acids 1-42) that was initially monomerized by HFIP-treatment and then allowed to form oligomers by the procedure described in Youmans KL et al. , 2012 , followed by lyophilisation using Biosensis' proprietary stabilization procedures. The resulting oligomeric mixture has been specially designed to allow the formation of stable, oligomeric A? 1-42 peptide, multimeric complexes or oligomers. The material is intended to be used as a stable and consistent standard or positive control for oligomeric ELISA assays, as well as other research applications.
Product Type:
Peptide
Format:
Lyophilized, Supplied as 2 x 500 ng vials, each containing lyophilized A? oligomers</b>. Note that the amount of provided oligomeric protein is based on the amount of monomeric A? used to form these oligomers. The precise formation, size and number of oligomers cannot be quantified by any known method.
Applications:
ELISA
Application Details:
<i>Use as positive control in Oligomeric A? ELISA Kit (BEK-2215)</i>: Reconstitute one vial with 1 mL of assay buffer provided in the ELISA kit. Dilute to a concentration of 0.5-1 ng/mL. At this concentration, a positive signal will be obtained within the dynamic range of the calibration curve.<br><br><i>Use as oligomeric A-beta peptide standard in Oligomeric A? ELISA Kit (BEK-2215)</i>: Reconstitute one vial with 1 mL of assay buffer provided in the ELISA kit. Dilute to a concentration of 2 ng/mL, which represents the highest concentration of the calibration curve. Perform a 1:2 serial dilution down to 0.031 ng/mL in assay buffer. Click <a class="newA" target="_blank" href="https://www.biosensis.com/documents/enhancedinfo/PE-1750-1000_Instructions for Generating a Calibration Curve.pdf"> here </a> for detailed instructions on generating a calibration curve with PE-1750-1000.<br><br><i>Use as positive control in other applications</i>: Optimal concentrations need to be determined empirically. It is recommended to reconstitute the vial with 100 - 200 µL buffer first (eg., PBS, pH 7.4), and prepare further working dilutions thereof.
Store unopened, lyophilized oligomeric A? with desiccant, insulated, at -20°C short term, -80°C long term. Store reconstituted vial at 2-8°C for up to 2 days. The reconstituted material should not be frozen for best results.
Synthetic beta-amyloid A? 1-42 was monomerized by HFIP (hexafluoro-2-propanol) treatment and dried. One vial contains 50 ?g monomeric A? peptide that can be used to form solutions of unaggregated A? monomers, aggregated A? oligomers, A? fibrils and A? protein complexes according to published protocols, and used in a variety of research applications.
Product Type:
Peptide
Format:
Lyophilized.
Applications:
ELISA
Application Details:
<i>Preparation of unaggregated A-beta<sub>1-42</sub></i>:<br><br><b>Important:</b> unaggregated A-beta has to be prepared just prior to use!<br><br>1. Add 5 µL of reconstituting buffer to one vial of 50 µg of HFIP-treated A-beta peptide; spin down the liquid briefly<br>2. Vortex the vial for 5 seconds at highest speed while rotating the vial with your hands; spin down the liquid (bench-top microcentrifuge) and repeat the vortex-spin procedure for a minimum of 3 times; continue the vortex-spin procedure until all lyophilized peptide is dissolved and collected at the bottom of the tube. <b>Important</b>: refer to the attached <a class="newA" target="_blank" href="https://www.biosensis.com/documents/enhancedinfo/PE-1749-50_Peptide Reconstitution Instructions.pdf">instructions</a> for a detailed procedure to ensure that all peptide is fully reconstituted!<br>3. Add 106 µL of cold Dilution Buffer to make up to 111 µL total volume and a peptide concentration of 100 µM. Vortex-spin for 3 more times<br> 4. Final concentration of A-beta is 450 µg/mL<br>5. Use reconstituted peptide <b>immediately</b> to avoid oligomer formation<br><br><i>Preparation of oligomeric A-beta<sub>1-42</sub></i>:<br><br>1. Add 5 µL of reconstituting buffer to one vial of 50 µg of HFIP-treated A-beta peptide; spin down the liquid briefly<br>2. Vortex the vial for 5 seconds at highest speed while rotating the vial with your hands; spin down the liquid (bench-top microcentrifuge) and repeat the vortex-spin procedure for a minimum of 3 times; continue the vortex-spin procedure until all lyophilized peptide is dissolved and collected at the bottom of the tube. <b>Important</b>: refer to the attached <a class="newA" target="_blank" href="https://www.biosensis.com/documents/enhancedinfo/PE-1749-50_Peptide Reconstitution Instructions.pdf">instructions</a> for a detailed procedure to ensure that all peptide is fully reconstituted!<br>3. Add 106 µL of cold Dilution Buffer to make up to 111 µL total volume and a peptide concentration of 100 µM<br>4. Vortex-spin for 3 more times<br>5. Incubate the solution at 2-8ºC for 24 hours (protected from light)<br>6. Final concentration of A-beta is 450 µg/mL<br>7. Once reconstituted and oligomerized, o-A-beta should be used as soon as possible and within 7 days to ensure the stability of the oligomers<br><br><b>Note:</b> while the concentration of monomeric A-beta peptide used to form the oligomeric complexes is accurately determined, the precise formation, size and number of oligomers cannot be quantified by any known method.<br><br><i>Preparation of fibrillar A-beta<sub>1-42</sub></i>:<br><br>1. Add 5 µL of reconstituting buffer to one vial of 50 µg of HFIP-treated A-beta peptide; spin down the liquid briefly<br>2. Vortex the vial for 5 seconds at highest speed while rotating the vial with your hands; spin down the liquid (bench-top microcentrifuge) and repeat the vortex-spin procedure for a minimum of 3 times; continue the vortex-spin procedure until all lyophilized peptide is dissolved and collected at the bottom of the tube. <b>Important:</b> refer to the attached <a class="newA" target="_blank" href="https://www.biosensis.com/documents/enhancedinfo/PE-1749-50_Peptide Reconstitution Instructions.pdf">instructions</a> for a detailed procedure to ensure that all peptide is fully reconstituted!<br>3. Add 106 µL of 10 mM HCl to make up to 111 µL total volume and a peptide concentration of 100 µM<br>4. Vortex-spin for 3 more times<br> 5. Incubate the solution at 37ºC for 24 hours (protected from light)<br>6. Final concentration of A-beta is 450 µg/mL<br><br><i>Preparation of A-beta<sub>1-42</sub> Complexes</i>:<br><br><b>Important:</b> only unagreggated A-beta will form complexes. Use A-beta peptide immediately after reconstitution to form complexes.<br><br>1. Add 5 µL of reconstituting buffer to one vial of 50 µg of HFIP-treated A-beta peptide; spin down the liquid briefly<br>2. Vortex the vial for 5 seconds at highest speed while rotating the vial with your hands; spin down the liquid (bench-top microcentrifuge) and repeat the vortex-spin procedure for a minimum of 3 times; continue the vortex-spin procedure until all lyophilized peptide is dissolved and collected at the bottom of the tube. <b>Important:</b> refer to the attached <a class="newA" target="_blank" href="https://www.biosensis.com/documents/enhancedinfo/PE-1749-50_Peptide Reconstitution Instructions.pdf">instructions</a> for a detailed procedure to ensure that all peptide is fully reconstituted!<br>3. Add 106 µL of cold Dilution Buffer to make up to 111 µL total volume and a peptide concentration of 100 µM<br> 4. Vortex-spin for 3 more times<br> 5. Use reconstituted peptide <b>immediately</b> to avoid oligomer formation<br>6. Mix the A-beta monomer with its complex partner (eg., lipoprotein) at desired concentrations in PBS, pH 7.4, or other suitable buffers compatible with its intended application<br>7. Incubate at room temperature for 2 hours without shaking<br>8. Use complexes immediately after incubation<br><br>These protocols are based on procedures published by <a class="newA" target="_blank" href="https://www.ncbi.nlm.nih.gov/pubmed/22423893">Youmans KL <i>et al.</i>, 2012</a> and <a class="newA" target="_blank" href="https://www.ncbi.nlm.nih.gov/pubmed/23293020">Tai LM <i>et al.</i>, 2013</a>, and we refer to these publications and other relevant literature for further details.<br><br>Provided working concentrations are only meant to guide the user. Optimal concentrations depend on the experimental design and need to be determined empirically.
Nerve growth factor (NGF) receptor, also known as p75NTR, is a low affinity NGF receptor. It binds with equal affinity all members of the neurotrophin family including beta NGF, BDNF, NT-3 and NT-4/5. It also binds the pro-neurotrophins. NGF receptors mediate signaling of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. These receptors are also thought to play a role in neurogenerative diseases such as Alzheimers disease. The p75NTR NGF receptor is a type I transmembrane glycoprotein (396 aa) consisting of a signal peptide (21 aa), an extracellular domain (225 aa) which contains four cysteine rich domains responsible for ligand binding, a transmembrane domain (19 aa) and a cytoplasmic domain (152 aa). It is a member of the TNF-alpha receptor family (TNR16). Recently, p75NTR has been shown to act as a receptor for many pathogens such as prions, rabies virus and amyloid beta. It also acts as a co-receptor for NOGO, mediating inhibitory signals of myelin associated protein.
Lyophilized products should be stored at 2-8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2-IN-1), Purified Small Molecule, suitable to Block.
Background Info:
Leucine-Rich Repeat Kinase 2 (LRRK2) is a member of the leucine-rich repeat kinase family. Mutations in the LRRK2 gene have been linked with late-onset autosomal dominant Parkinson's disease (PD). A recent screening identified the analog LRRK2-IN-1 as a potent and selective inhibitor of LRRK2. This research by Deng et al (2011, Nat Chem Biol) showed that LRRK2-IN-1 suppressed LRRK2 kinase activity in vivo leading to dephosphorylation of Ser910/Ser935, loss of 14-3-3 binding and accumulation of LRRK2 within aggregrate fibrillar structures. LRRK2-IN-1, is the first selective and potent inhibitor of LRRK2. LRRK2-IN-1 inhibits both wild-type and G2019S mutant LRRK2 kinase activity with IC50 values of 13nM and 6nM respectively (Deng et al 2011, Nat Chem Biol).
Product Type:
Small Molecule
Format:
Dry, Synthetic chemical powder
Applications:
Block
Application Details:
Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Deng X. et al (2011) Characterization of a selective inhibitor of the Parkinson's disease kinase LRRK2 Nat Chem Biol. 2011 Apr;7(4):203-5.
Storage:
Store dry, unopened vial protected from light for up to 3 months at RT or at -70ºC for up to 1 year. Reconstitute product in 100% ACS grade DMSO and store at -80ºC, in aliquots, protected from light, for up to 1 month. Prevent multiple freeze-thaw cycles. CAS Number 1234480-84-2.
Human LR3 insulin-like Growth Factor-I (LR3IGF-I) was developed by GroPep Bioreagents ( www.gropep.com ) specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR3IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR3IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules, which are responsible for promoting cell survival by inhibition of apoptosis. This LR3IGF-I Rapid ELISA kit combines GroPep's many years of expertise in the field of IGF research and Biosensis' newly established Rapid ELISA platform. This collaboration has resulted in the new LR3IGF-I Rapid ELISA kit, which provides for the sensitive, specific and reliable quantification of LR3IGF-I protein in less than 3 hours! The ELISA kit consists of a complete set of reagents and pre-coated plate to allow immediate assay of LR3IGF-I in culture media. Included in the kit are mouse monoclonal anti- LR3IGF-I capture antibody pre-coated onto an ELISA plate, standard LR3IGF-I protein, a biotinylated anti- LR3IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of LR3IGF-I present in samples and the supplied protein standard. This LR3IGF-I Rapid ELISA kit has been developed, optimized and validated to quantify LR3IGF-I protein in cell culture medium. It is likely to be used to measure LR3IGF-I in media and during downstream processing of media following a production cycle and is not intended for other use. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
LR3IGF-I is an 83 amino acid analogue of IGF-I comprising the complete human IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3, plus a 13 amino acid extension peptide at the N-terminus.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant LR3-IGF1 protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of LR3IGF-I in Culture Supernatant. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Human LR3 insulin-like Growth Factor-I.
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Specificity:
Human The assay is intended for quantification of LR3IGF-I. Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%.
Storage:
Store at 2-8°C
Range:
3.9 ng/mL - 200 ng/mL
Sample Type:
Culture Supernatant
Sensitivity:
Typical limit of detection (LOD) for LR3IGF-I is 1 ng/mL determined as 150% of the blank value.
Cross Reactivity:
Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%.
Human LR3 insulin-like Growth Factor-I (LR3IGF-I) was developed by GroPep Bioreagents ( www.gropep.com ) specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR3IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR3IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules, which are responsible for promoting cell survival by inhibition of apoptosis. This LR3IGF-I Rapid ELISA kit combines GroPep's many years of expertise in the field of IGF research and Biosensis' newly established Rapid ELISA platform. This collaboration has resulted in the new LR3IGF-I Rapid ELISA kit, which provides for the sensitive, specific and reliable quantification of LR3IGF-I protein in less than 3 hours! The ELISA kit consists of a complete set of reagents and pre-coated plate to allow immediate assay of LR3IGF-I in culture media. Included in the kit are mouse monoclonal anti- LR3IGF-I capture antibody pre-coated onto an ELISA plate, standard LR3IGF-I protein, a biotinylated anti- LR3IGF-I detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of LR3IGF-I present in samples and the supplied protein standard. This LR3IGF-I Rapid ELISA kit has been developed, optimized and validated to quantify LR3IGF-I protein in cell culture medium. It is likely to be used to measure LR3IGF-I in media and during downstream processing of media following a production cycle and is not intended for other use. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
LR3IGF-I is an 83 amino acid analogue of IGF-I comprising the complete human IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3, plus a 13 amino acid extension peptide at the N-terminus.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant LR3-IGF1 protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of LR3IGF-I in Culture Supernatant. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Human LR3 insulin-like Growth Factor-I.
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Specificity:
Human The assay is intended for quantification of LR3IGF-I. Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%.
Storage:
Store at 2-8°C
Range:
3.9 ng/mL - 200 ng/mL
Sample Type:
Culture Supernatant
Sensitivity:
Typical limit of detection (LOD) for LR3GF-I is 1 ng/mL determined as 150% of the blank value.
Cross Reactivity:
Cross-reaction with human IGF-I is 32%. Cross-reaction with human IGF-II is less than 0.01%.
The biosensis Multi-Neurotrophin Rapid TM Screening ELISA kit has been designed to allow rapid screening and quantification of rat NGF, BDNF, NT3 and NT4/5 in cell culture supernatants, lysates, serum and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular rat serum. This two-plate kit consists of four sets of 6 strips for each Neurotrophin, allowing for the assay of 16 samples per Neurotrophin tested and a full range of standards, all run in duplicate. It provides the identical sensitivities and ranges that are achieved in the complete, individual ELISA kits, thus allowing easy progression into the larger individual assay sizes when needed. The Multi-Neurotrophin Rapid TM Screening ELISA kit therefore presents a cost effective way to quickly screen multiple samples, which can then be published or used prior to a more extensive analysis with the individual kits. The kit comes complete with all detection reagents and is ready to use. Individual coated strips are provided and each set of standards and detection antibodies are color-coded. NOTE: For research use only. Not for diagnostic and clinical purposes.
Background Info:
The Neurotrophin family of growth factors in all mammals including human has four members including Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF), Neurotrophin 3 (NT3) and Neurotrophin 4/5 (NT4/5). These are all essential to brain development, maturation and adult function, particularly for cell differentiation, survival, death and synaptic regulation.
Product Type:
ELISA Assay
Species Reactivity:
Rat
Immunogen:
As for individual ELISA kits
Applications:
ELISA
Application Details:
ELISA. For the quantification of Multi-Neurotrophin Screening in Culture Supernatant, Cell Lysates, Serum, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
NGF; BDNF; NT3; NT4/5;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s) (6 strips/48 wells per neurotrophin target), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Kim GB et al. (2018) "The critical chemical and mechanical regulation of folic acid on neural engineering." Biomaterials. [Epub ahead of print] Application: Rat Schwann cell culture supernatants.
Specificity:
This Multi-Neurotrophin Screening kit uses the same antibodies as in the individual Rapid ELISA kits. Each kit has been tested for cross-reactivity with other closely related neurotrophins and no cross-reactivity has been observed at 25 ng/mL of each neurotrophin tested. The antibodies used in this kit detect the human and rat form of each neurotrophin, but with varying efficiency depending on the target protein.
The biosensis Multi-Neurotrophin Rapid TM Screening ELISA kit has been designed to allow rapid screening and quantification of mouse NGF, BDNF, NT3 and NT4/5 in cell culture supernatants, lysates and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This two-plate kit consists of four sets of 6 strips for each Neurotrophin, allowing for the assay of 16 samples per Neurotrophin tested and a full range of standards, all run in duplicate. It provides the identical sensitivities and ranges that are achieved in the complete, individual ELISA kits, thus allowing easy progression into the larger individual assay sizes when needed. The Multi-Neurotrophin Rapid TM Screening ELISA kit therefore presents a cost effective way to quickly screen multiple samples, which can then be published or used prior to a more extensive analysis with the individual kits. The kit comes complete with all detection reagents and is ready to use. Individual coated strips are provided and each set of standards and detection antibodies are color-coded. NOTE: For research use only. Not for diagnostic and clinical purposes.
Background Info:
The Neurotrophin family of growth factors in all mammals including human has four members including Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF), Neurotrophin 3 (NT3) and Neurotrophin 4/5 (NT4/5). These are all essential to brain development, maturation and adult function, particularly for cell differentiation, survival, death and synaptic regulation.
Product Type:
ELISA Assay
Species Reactivity:
Mouse
Immunogen:
As for individual ELISA kits
Applications:
ELISA
Application Details:
ELISA. For the quantification of Multi-Neurotrophin Screening in Culture Supernatant, Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
NGF; BDNF; NT3; NT4/5;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s) (6 strips/48 wells per neurotrophin target), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Kawanokuchi J et al. (2021) Acupuncture Treatment for Social Defeat Stress. Front Behav Neurosci. 15:685433. Application: Brain homogenate. Takagishi S et al. (2021) Protein nanoparticles modified with PDGF-B as a novel therapy after acute cerebral infarction. eNeuro. [Epub ahead of print]. Application: Brain homogenate. Leitao L et al. (2020) Osteoblasts are inherently programmed to repel sensory innervation. Bone Res. 8:20. Application: Culture supernatant. Yamada K et al. (2020) The impact of ovariectomy on olfactory neuron regeneration in mice. Chem Senses. [Epub ahead of print]. Application: Mouse olfactory bulb homogenate. Hutson TH et al. (2019) Cbp-dependent histone acetylation mediates axon regeneration induced by environmental enrichment in rodent spinal cord injury models. Sci Transl Med. 11(487). Application: Rodent DRG lysates. Miura-Yura E et al. (2019) Secreted factors from cultured dental pulp stem cells promoted neurite outgrowth of dorsal root ganglion neurons and ameliorated neural functions in streptozotocin-induced diabetic mice. J Diabetes Investig. [Epub ahead of print]. Application: Conditioned medium of stem cells from human exfoliated deciduous teeth. Noda T et al. (2019) Effects of Tokishakuyakusan on regeneration of murine olfactory neurons in vivo and in vitro. Chem Senses. [Epub ahead of print]. Application: Mouse olfactory bulb homogenate.
Specificity:
This Multi-Neurotrophin Screening kit uses the same antibodies as in the individual Rapid ELISA kits. Each kit has been tested for cross-reactivity with other closely related neurotrophins and no cross-reactivity has been observed at 25 ng/mL of each neurotrophin tested. The antibodies used in this kit detect the human and rat form of each neurotrophin, but with varying efficiency depending on the target protein.
The Biosensis GDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular rat serum samples. This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. This GDNF ELISA kit employs a recombinant rat GDNF standard and is therefore designed to measure the rat form of GDNF. Note that the antibodies used in this kit cross-react with mouse, human and guinea pig GDNF. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
ELISA Assay
Species Reactivity:
Rat
Immunogen:
Recombinant rat GDNF protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Glial cell line-derived neurotrophic factor (GDNF) in Culture Supernatant, Cell Lysates, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Possamai-Della T et al. (2022) Imipramine Can Be Effective on Depressive-Like Behaviors, but Not on Neurotrophic Factor Levels in an Animal Model for Bipolar Disorder Induced by Ouabain Mol Neurobiol. [Epub ahead of print] Application: Rat, brain tissue homogenate. Castro SL et al. (2022) Blueberry Juice Augments Exercise-Induced Neuroprotection in a Parkinsons Disease Model Through Modulation of GDNF Levels. IBRO Neurosci Rep. [Epub ahead of print] Application: Rat, brain tissue homogenate.
Specificity:
Rat. The antibodies used in this kit detect human and mouse GDNF. The assay antibodies cross-react with guinea pig GDNF as evidenced by measuring GDNF in guinea pig serum with the Human GDNF Rapid TM ELISA kit . All Biosensis GDNF Rapid TM ELISA kits use the same capture and detection antibodies, and thus this Rat GDNF Rapid TM ELISA kit can be used to assay guinea pig GDNF in serum. However, in absence of a true guinea pig GDNF protein standard, the Human GDNF Rapid TM ELISA kit may give the most accurate estimations of guinea pig GDNF levels based on amino acid sequence homology. No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
Storage:
Store at 2-8°C.
Range:
7.8 - 500 pg/mL
Sample Type:
Cell Lysates,Culture Supernatant,Serum
Sensitivity:
Typical limit of detection (LOD) for rrGDNF is <5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant Human Neurturin, Artemin and Persephin.
The Biosensis GDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular rat serum samples. This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. This GDNF ELISA kit employs a recombinant rat GDNF standard and is therefore designed to measure the rat form of GDNF. Note that the antibodies used in this kit cross-react with mouse, human and guinea pig GDNF. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
ELISA Assay
Species Reactivity:
Rat
Immunogen:
Recombinant rat GDNF protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Glial cell line-derived neurotrophic factor (GDNF) in Culture Supernatant, Cell Lysates, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Possamai-Della T et al. (2022) Imipramine Can Be Effective on Depressive-Like Behaviors, but Not on Neurotrophic Factor Levels in an Animal Model for Bipolar Disorder Induced by Ouabain Mol Neurobiol. [Epub ahead of print] Application: Rat, brain tissue homogenate. Castro SL et al. (2022) Blueberry Juice Augments Exercise-Induced Neuroprotection in a Parkinsons Disease Model Through Modulation of GDNF Levels. IBRO Neurosci Rep. [Epub ahead of print] Application: Rat, brain tissue homogenate.
Specificity:
Rat. The antibodies used in this kit detect human and mouse GDNF. The assay antibodies cross-react with guinea pig GDNF as evidenced by measuring GDNF in guinea pig serum with the Human GDNF Rapid TM ELISA kit . All Biosensis GDNF Rapid TM ELISA kits use the same capture and detection antibodies, and thus this Rat GDNF Rapid TM ELISA kit can be used to assay guinea pig GDNF in serum. However, in absence of a true guinea pig GDNF protein standard, the Human GDNF Rapid TM ELISA kit may give the most accurate estimations of guinea pig GDNF levels based on amino acid sequence homology. No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
Storage:
Store at 2-8°C.
Range:
7.8 - 500 pg/mL
Sample Type:
Cell Lysates,Culture Supernatant,Serum
Sensitivity:
Typical limit of detection (LOD) for rrGDNF is <5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant Human Neurturin, Artemin and Persephin.
The Biosensis GDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants and cell lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. This GDNF ELISA kit employs a recombinant mouse GDNF standard and is therefore designed to measure the murine form of GDNF. Note that the antibodies used in this kit cross-react with rat, human and guinea pig GDNF. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
ELISA Assay
Species Reactivity:
Mouse
Immunogen:
Recombinant mouse GDNF protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Glial cell line-derived neurotrophic factor (GDNF) in Culture Supernatant and Cell Lysates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Specificity:
Mouse. The antibodies used in this kit detect human and rat GDNF. The assay antibodies cross-react with guinea pig GDNF as evidenced by measuring GDNF in guinea pig serum with the Human GDNF Rapid TM ELISA kit . All Biosensis GDNF Rapid TM ELISA kits use the same capture and detection antibodies, and thus this Mouse GDNF Rapid TM ELISA kit can be used to assay guinea pig GDNF in serum. However, in absence of a true guinea pig GDNF protein standard, the Human GDNF Rapid TM ELISA kit may give the most accurate estimations of guinea pig GDNF levels based on amino acid sequence homology. No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
Storage:
Store at 2-8°C.
Range:
7.8 - 500 pg/mL
Sample Type:
Cell Lysates,Culture Supernatant
Sensitivity:
Typical limit of detection (LOD) for rmGDNF is less than 5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant Human Neurturin, Artemin and Persephin.
The Biosensis GDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants and cell lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. This GDNF ELISA kit employs a recombinant mouse GDNF standard and is therefore designed to measure the murine form of GDNF. Note that the antibodies used in this kit cross-react with rat, human and guinea pig GDNF. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
ELISA Assay
Species Reactivity:
Mouse
Immunogen:
Recombinant mouse GDNF protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Glial cell line-derived neurotrophic factor (GDNF) in Culture Supernatant and Cell Lysates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Specificity:
Mouse. The antibodies used in this kit detect human and rat GDNF. The assay antibodies cross-react with guinea pig GDNF as evidenced by measuring GDNF in guinea pig serum with the Human GDNF Rapid TM ELISA kit . All Biosensis GDNF Rapid TM ELISA kits use the same capture and detection antibodies, and thus this Mouse GDNF Rapid TM ELISA kit can be used to assay guinea pig GDNF in serum. However, in absence of a true guinea pig GDNF protein standard, the Human GDNF Rapid TM ELISA kit may give the most accurate estimations of guinea pig GDNF levels based on amino acid sequence homology. No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
Storage:
Store at 2-8°C.
Range:
7.8 - 500 pg/mL
Sample Type:
Cell Lysates,Culture Supernatant
Sensitivity:
Typical limit of detection (LOD) for rmGDNF is less than 5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
The Biosensis Rat Mature NGF/proNGF Combo Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit combines individual, but complementary ELISA kits for the two most important NGF isoforms: Mature NGF (BEK-2214) and full-length proNGF (BEK-2236). Both kits are sandwich ELISAs, useful for the quantification of mature NGF and proNGF in less than 4 hours in cell culture supernatants and tissue homogenates only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Please refer to the most current kit protocols for BEK-2214 (Mature BDNF Rapid TM ELISA) and BEK-2236 (proNGF Rapid TM ELISA), for specific use instructions for each substrate application. The Mature NGF ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The proNGF ELISA kit consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature NGF or proNGF present in samples and protein standards. A NGF and proNGF positive control (QC sample) is provided to assure consistent assay performance. The Mature NGF ELISA kit employs a high-quality recombinant rat NGF protein as standard, and thus the Mature NGF/proNGF Combo kit is designed to measure the rat NGF isoforms. However, the rat mature NGF ELISA kit will cross-react with mouse and human mature NGF protein. The proNGF ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli. Mouse proNGF and rat proNGF share 96% sequence homology, and internal validation data has demonstrated the ability of the proNGF ELISA to detect proNGF in rat PC12 cell lysates and rat brain tissue homogenate. However, the proNGF ELISA shows only little cross-reactivity (20%) with human proNGF. This Combo kit is capable of distinguishing and independently quantifying the mature NGF and full-length NGF isoforms. Internal validation data demonstrates that the mature NGF ELISA assay antibodies preferentially detect the mature form of NGF. Cross-reaction of full-length proNGF was This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
Product Type:
ELISA Assay
Species Reactivity:
Rat
Immunogen:
See BEK-2214 & BEK-2236 for specific details
Applications:
ELISA
Application Details:
ELISA. For the quantification of Mature NGF and proNGF in Culture Supernatant, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 2 x 96-well pre-coated strip plates per kit (1 x NGF antibody, 1 x proNGF antibody coated plate), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Please refer to BEK-2214 (Rat Mature NGF Rapid TM ELISA Kit) and BEK-2236 (Mouse/Rat proNGF Rapid TM ELISA Kit) .
Specificity:
Mature NGF ELISA: Detects rat NGF, and cross-reacts with human and mouse mature NGF. This mature NGF ELISA preferentially detects mature NGF over full-length proNGF. proNGF ELISA: Detects mouse and rat proNGF only, and shows only little reactivity (20%) with human proNGF. The proNGF ELISA detects the full-length form of proNGF only, and does not quantify mature NGF or the pro-domain peptide.
Typical limit of detection (LOD) for rat mature NGF is 2 pg/mL determined as 150% of the blank value. Typical limit of detection (LOD) for rat proNGF is less than 50 pg/mL, determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
<b>Cross-reactivity of NGF isoforms:</b><br><br><b>Mature NGF ELISA:</b><br>The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and NT-4/5 tested at 25 ng/mL in assay buffer. Mature NGF (27 kDa) and full-length proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the mature NGF ELISA calibration range (3.9-250 pg/mL; 0.14-9.2 pmol/L). OD readings for proNGF were indistinguishable from the assay's blank OD readings.<br><br><b>proNGF ELISA:</b><br>Does not cross-react with proBDNF and mature NGF. Mature NGF spiked into brain homogenate does not interfere with proNGF quantification.
The biosensis Multi-Neurotrophin Rapid TM Screening ELISA kit has been designed to allow rapid screening and quantification of human NGF, BDNF, NT3 and NT4/5 in cell culture supernatants, lysates, serum, plasma (EDTA and citrate) and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human serum and plasma samples. This two-plate kit consists of four sets of 6 strips for each Neurotrophin, allowing for the assay of 16 samples per Neurotrophin tested and a full range of standards, all run in duplicate. It provides the identical sensitivities and ranges that are achieved in the complete, individual ELISA kits, thus allowing easy progression into the larger individual assay sizes when needed. The Multi-Neurotrophin Rapid TM Screening ELISA kit therefore presents a cost effective way to quickly screen multiple samples, which can then be published or used prior to a more extensive analysis with the individual kits. The kit comes complete with all detection reagents and is ready to use. Individual coated strips are provided and each set of standards and detection antibodies are color-coded. NOTE: For research use only. Not for diagnostic and clinical purposes.
Background Info:
The Neurotrophin family of growth factors in all mammals including human has four members including Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF), Neurotrophin 3 (NT3) and Neurotrophin 4/5 (NT4/5). These are all essential to brain development, maturation and adult function, particularly for cell differentiation, survival, death and synaptic regulation.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
As for individual ELISA kits
Applications:
ELISA
Application Details:
ELISA. For the quantification of Multi-Neurotrophin Screening in Culture Supernatant, Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
NGF; BDNF; NT3; NT4/5;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s) (6 strips/48 wells per neurotrophin target), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Lamb WDB (2021) "Potential of Muller glia-derived extracellular vesicles for retinal neuroprotection." PhD Thesis Application: Human cell culture supernatant. Elisa A et al. (2021) "Aging is associated with cardiac autonomic nerve fiber depletion and reduced cardiac and circulating BDNF levels." J Geriatr Cardiol. 18(7): 549559 Application: Rat serum. Woo JH et al. (2019) "Effect of Dehydrocostus Lactone Isolated from the Roots of Aucklandia lappa on the Apoptosis of Endometriotic Cells and the Alternative Activation of Endometriosis-Associated Macrophages." Am J Chin Med. [Epub ahead of print] Application: Human cell culture supernatants. Kalinowska-Lyszczarz A et al. (2018) "Immune-cell BDNF expression in treatment-naive relapsing-remitting multiple sclerosis patients and following one year of immunomodulation therapy." Neurol Neurochir Pol. [In Press] Application: Human PBMC cell lysates. Lindsay SL et al. (2016) "Comparative miRNA-Based Fingerprinting Reveals Biological Differences in Human Olfactory Mucosa- and Bone-Marrow-Derived Mesenchymal Stromal Cells." Stem Cell Reports. 6(5):729-42 Application: MSC-conditioned medium/cell supernatant. Carnevale G et al. (2016) "Human dental pulp stem cells expressing STRO-1, c-kit and CD34 markers in peripheral nerve regeneration." J Tissue Eng Regen Med. [Epub ahead of print] Application: Supernatant of human dental pulp stem cells (hDPSCs).
Specificity:
This Multi-Neurotrophin Screening kit uses the same antibodies as in the individual Rapid ELISA kits. Each kit has been tested for cross-reactivity with other closely related neurotrophins and no cross-reactivity has been observed at 25 ng/mL of each neurotrophin tested. The antibodies used in this kit detect the mouse and rat form of each neurotrophin, but with varying efficiency depending on the target protein.
The Biosensis proNGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of full-length proNGF protein in less than 4 hours in human serum, heparin-plasma, cell supernatants and cell lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. A human proNGF positive control (QC sample) is provided to assure consistent assay performance. This proNGF ELISA kit contains a recombinant human proNGF standard expressed in E.coli. This ELISA kit does not cross-react with the mouse form of proNGF, and due to sequence homology of mouse and rat proNGF is not expected to detect rat proNGF. The antibodies used in this ELISA kit bind epitopes within the pro-domain of the protein and therefore recognize proNGF and the pro-domain peptide, but do not cross-react with mature NGF! Note that accurate proNGF quantification in human serum requires the addition of Heterophilic Antibody Blocker BL-003-1000 provided in the kit, and available for purchase separately . This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant human, wild-type proNGF protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor, pro- (proNGF) in Serum, Plasma (Heparin), Culture Supernatant, Cell Lysates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
pro-beta nerve growth factor; proNGF; NGF
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
March B et al. (2021). ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine. Mossa AH et al. (2020). Imbalance of nerve growth factor metabolism in aging women with overactive bladder syndrome. World J Urol. [Epub ahead of print]. Application: Human urine. Rowe CW et al. (2019). The precursor for nerve growth factor (proNGF) is not a serum or biopsy-rinse biomarker for thyroid cancer diagnosis. BMC Endocr Disord. 19(1):128. Application: Human serum. Stapledon CJM et al. (2019). Human osteocyte expression of Nerve Growth Factor: The effect of Pentosan Polysulphate Sodium (PPS) and implications for pain associated with knee osteoarthritis. PLoS One. 14(9):e0222602. Application: Human primary culture supernatant. Ryu JC et al. (2018). Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. [Epub ahead of print]. Application: Human urine. Sherif IO & Al-Gayyar MMH (2018). Oleuropein potentiates anti-tumor activity of cisplatin against HepG2 through affecting proNGF/NGF balance. Life Sci. [Epub ahead of print]. Application: Human cell culture.
Specificity:
Human. Does not react with mouse proNGF, and is not expected to detect rat proNGF. Does not cross-react with recombinant human NGF and proBDNF tested at 25 ng/mL.
The Biosensis proNGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of full-length proNGF protein in less than 4 hours in human serum, heparin-plasma, cell supernatants and cell lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. A human proNGF positive control (QC sample) is provided to assure consistent assay performance. This proNGF ELISA kit contains a recombinant human proNGF standard expressed in E.coli. This ELISA kit does not cross-react with the mouse form of proNGF, and due to sequence homology of mouse and rat proNGF is not expected to detect rat proNGF. The antibodies used in this ELISA kit bind epitopes within the pro-domain of the protein and therefore recognize proNGF and the pro-domain peptide, but do not cross-react with mature NGF! Note that accurate proNGF quantification in human serum requires the addition of Heterophilic Antibody Blocker BL-003-1000 provided in the kit, and available for purchase separately . This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant human, wild-type proNGF protein, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor, pro- (proNGF) in Serum, Plasma (Heparin), Culture Supernatant, Cell Lysates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
pro-beta nerve growth factor; proNGF; NGF
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
March B et al. (2021). ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine. Mossa AH et al. (2020). Imbalance of nerve growth factor metabolism in aging women with overactive bladder syndrome. World J Urol. [Epub ahead of print]. Application: Human urine. Rowe CW et al. (2019). The precursor for nerve growth factor (proNGF) is not a serum or biopsy-rinse biomarker for thyroid cancer diagnosis. BMC Endocr Disord. 19(1):128. Application: Human serum. Stapledon CJM et al. (2019). Human osteocyte expression of Nerve Growth Factor: The effect of Pentosan Polysulphate Sodium (PPS) and implications for pain associated with knee osteoarthritis. PLoS One. 14(9):e0222602. Application: Human primary culture supernatant. Ryu JC et al. (2018). Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. [Epub ahead of print]. Application: Human urine. Sherif IO & Al-Gayyar MMH (2018). Oleuropein potentiates anti-tumor activity of cisplatin against HepG2 through affecting proNGF/NGF balance. Life Sci. [Epub ahead of print]. Application: Human cell culture.
Specificity:
Human. Does not react with mouse proNGF, and is not expected to detect rat proNGF. Does not cross-react with recombinant human NGF and proBDNF tested at 25 ng/mL.
The Biosensis Mouse Mature NGF/proNGF Combo Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit combines individual, but complementary ELISA kits for the two most important NGF isoforms: Mature NGF (BEK-2213) and full-length proNGF (BEK-2236). Both kits are sandwich ELISAs, useful for the quantification of mature NGF and proNGF in less than 4 hours in cell culture supernatants and tissue homogenates only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Researchers have used both kits for NGF/proNGF measurement in mouse urine, however, Biosensis has not yet internally validated the use of urine in both ELISA kits. End-users are strongly advised to perform essential validation experiments to assure accurate NGF/proNGF quantification in mouse urine. Please refer to the most current kit protocols for BEK-2213 (Mature BDNF Rapid TM ELISA) and BEK-2236 (proNGF Rapid TM ELISA), for specific use instructions for each substrate application. The Mature NGF ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The proNGF ELISA kit consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature NGF or proNGF present in samples and protein standards. A NGF and proNGF positive control (QC sample) is provided to assure consistent assay performance. The Mature NGF ELISA kit employs a native mouse NGF protein as standard, purified from mouse submaxillary glands according to published procedures. The calibrator standard reflects the native state of mouse NGF protein and has been chosen to give most accurate quantification of natural NGF protein levels in mouse samples. The proNGF ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli. Note that this Mature NGF/proNGF Combo kit is designed to measure the mouse protein forms, although due to sequence homology the Mature NGF ELISA kit will detect human and rat mature NGF. The proNGF ELISA kit cross-reacts with rat proNGF due to high degree of homology (96%) with mouse proNGF based on amino acid sequence, and the ability of this kit in detecting proNGF in rat PC12 cell lysates and rat brain tissue homogenate. However, the proNGF ELISA shows only little cross-reactivity (20%) with human proNGF. This Combo kit is capable of distinguishing and independently quantifying the mature NGF and full-length NGF isoforms. Internal validation data demonstrates This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
Product Type:
ELISA Assay
Species Reactivity:
Mouse
Immunogen:
See BEK-2213 & BEK-2236 for specific details
Applications:
ELISA
Application Details:
ELISA. For the quantification of Mature NGF and proNGF in Culture Supernatant, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 2 x 96-well pre-coated strip plates per kit (1 x NGF antibody, 1 x proNGF antibody coated plate), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Please refer to BEK-2213 (Mouse Mature NGF Rapid TM ELISA Kit) and BEK-2236 (Mouse/Rat proNGF Rapid TM ELISA Kit) .
Specificity:
Mature NGF ELISA: Detects mouse NGF, and cross-reacts with human and rat mature NGF. This mature NGF ELISA preferentially detects mature NGF over full-length proNGF. proNGF ELISA: Detects mouse and rat proNGF only, and shows only little reactivity (20%) with human proNGF. The proNGF ELISA detects the full-length form of proNGF only, and does not quantify mature NGF or the pro-domain peptide.
Typical limit of detection (LOD) for mouse mature NGF is 2 pg/mL determined as 150% of the blank value. Typical limit of detection (LOD) for mouse proNGF is less than 50 pg/mL, determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
<b>Cross-reactivity of NGF isoforms:</b><br><br><b>Mature NGF ELISA:</b><br>The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and NT-4/5 tested at 25 ng/mL in assay buffer. Mature mouse NGF (27 kDa) and full-length mouse proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the Mouse NGF ELISA calibration range (3.9-250 pg/mL; 0.14-9.2 pmol/L). OD readings for mouse proNGF were indistinguishable from the assay's blank OD readings.<br><br><b>proNGF ELISA:</b><br>Does not cross-react with proBDNF and mature NGF. Mature NGF spiked into brain homogenate does not interfere with proNGF quantification.
Biosensis is proud to offer the first commercially available ApoE/?-amyloid (ApoE/A?) complex ELISA kit. As a result of extensive collaboration with Dr. LaDu's laboratory at UIC and validation by Biosensis, this ELISA can be used to accurately and consistently measure the extent of ApoE/A? complex in tissue extracts and other samples. The Biosensis ApoE/A? Complex ELISA kit is a sandwich ELISA and consists of a pre-coated mouse monoclonal anti-A? capture antibody, a highly validated ApoE/A? complex standard that is pre-formed, lyophilized and ready for reconstitution, a biotinylated ApoE detection antibody, and horseradish peroxidase (HRP)-conjugated streptavidin and detection reagent. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the level of ApoE/A? complex present in samples and protein standards. Importantly, a well-characterized and unique ApoE/A? complex is included as a standard. This complex is pre-formed and lyophilized, requiring only reconstitution with assay diluent prior to use. In order to assess non-specific ApoE protein binding, each kit includes additional plates pre-coated with control antibody. The purpose of this kit is the in vitro qualitative measurement of ApoE/A? complexes in brain extracts and CSF samples from both transgenic mice and humans or primates, relative to a known ApoE/A? complex standard, only if used as directed. This kit has not been tested for other sample applications. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Complex of E.coli-derived recombinant human ApoE protein and synthetic, monomerized Abeta (1-42) peptide
Applications:
ELISA
Application Details:
ELISA. For the quantification of Apolipoprotein E/beta-Amyloid Complex (ApoE/A beta) in CSF, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 2 x 96-well pre-coated strip plates per 1 Plate Kit (1 plate MOAB-2 antibody coated, 1 plate control antibody coated), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Tai LM et al. (2013) J Biol Chem. 288(8): 5914-26 Tai LM et al. (2014) Mol Neurodegen. 9:2
Specificity:
Human Apolipoprotein E/beta-Amyloid (ApoE/A beta) Complex. The kit has been assayed on human samples only but the capture antibody, MOAB-2, is know to react with rodent amyloid beta though weaker (20% less reactivity on dot blots). The polyclonal APOE used for detection should detect ApoE from a variety of species but so far has only been tested on human
Biosensis is proud to offer the first commercially available ApoE/?-amyloid (ApoE/A?) complex ELISA kit. As a result of extensive collaboration with Dr. LaDu's laboratory at UIC and validation by Biosensis, this ELISA can be used to accurately and consistently measure the extent of ApoE/A? complex in tissue extracts and other samples. The Biosensis ApoE/A? Complex ELISA kit is a sandwich ELISA and consists of a pre-coated mouse monoclonal anti-A? capture antibody, a highly validated ApoE/A? complex standard that is pre-formed, lyophilized and ready for reconstitution, a biotinylated ApoE detection antibody, and horseradish peroxidase (HRP)-conjugated streptavidin and detection reagent. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the level of ApoE/A? complex present in samples and protein standards. Importantly, a well-characterized and unique ApoE/A? complex is included as a standard. This complex is pre-formed and lyophilized, requiring only reconstitution with assay diluent prior to use. In order to assess non-specific ApoE protein binding, each kit includes additional plates pre-coated with control antibody. The purpose of this kit is the in vitro qualitative measurement of ApoE/A? complexes in brain extracts and CSF samples from both transgenic mice and humans or primates, relative to a known ApoE/A? complex standard, only if used as directed. This kit has not been tested for other sample applications. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Complex of E.coli-derived recombinant human ApoE protein and synthetic, monomerized Abeta (1-42) peptide
Applications:
ELISA
Application Details:
ELISA. For the quantification of Apolipoprotein E/beta-Amyloid Complex (ApoE/A beta) in CSF, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 2 x 96-well pre-coated strip plates per 1 Plate Kit (1 plate MOAB-2 antibody coated, 1 plate control antibody coated), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Tai LM et al. (2013) J Biol Chem. 288(8): 5914-26 Tai LM et al. (2014) Mol Neurodegen. 9:2
Specificity:
Human Apolipoprotein E/beta-Amyloid (ApoE/A beta) Complex. The kit has been assayed on human samples only but the capture antibody, MOAB-2, is know to react with rodent amyloid beta though weaker (20% less reactivity on dot blots). The polyclonal APOE used for detection should detect ApoE from a variety of species but so far has only been tested on human
The Biosensis Human Mature NGF/proNGF Combo Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit combines individual, but complementary ELISA kits for the two most important NGF isoforms: Mature NGF (BEK-2212) and full-length proNGF (BEK-2226). Both kits are sandwich ELISAs, useful for the quantification of mature NGF and proNGF in less than 4 hours in cell culture supernatants, serum, and plasma (citrate) only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Researchers have used both kits for NGF/proNGF measurement in human urine, however, Biosensis has not yet internally validated the use of urine in both ELISA kits. End-users are strongly advised to perform essential validation experiments to assure accurate NGF/proNGF quantification in human urine. Please refer to the most current kit protocols for BEK-2212 (Mature BDNF Rapid TM ELISA) and BEK-2226 (proNGF Rapid TM ELISA), for specific use instructions for each substrate application, in particular blood samples. The Mature NGF ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The proNGF ELISA kit consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature NGF or proNGF present in samples and protein standards. A NGF and proNGF positive control (QC sample) is provided to assure consistent assay performance. The Mature NGF ELISA kit employs a high-quality recombinant human NGF standard approved by the World Health Organization (WHO). The proNGF ELISA kit contains a recombinant human proNGF standard expressed in E.coli. Note that this Mature NGF/proNGF Combo kit is designed to measure the human protein forms, although due to sequence homology the Mature NGF ELISA kit will detect mouse and rat mature NGF. The proNGF ELISA kit does not cross-react with rodent forms of proNGF. This Combo kit is capable of distinguishing and independently quantifying the mature NGF and full-length NGF isoforms. Internal validation data demonstrates Mossa AH et al. (2020) . The antibodies used in the proNGF ELISA kit bind epitopes within the pro-domain of the protein and therefore recognize proNGF and the pro-domain peptide, but do not cross-react with mature NGF! Important: Accurate NGF quantification in human citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-005-500 . Accurate proNGF quantification in human serum requires the addition of Heterophilic Antibody Blocker BL-003-1000 . Both Heterophilic Antibody Blockers are provided in the kit. This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
See BEK-2212 & BEK-2226 for specific details
Applications:
ELISA
Application Details:
ELISA. For the quantification of Mature NGF and proNGF in Culture Supernatant, Serum, Plasma (Citrate). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 2 x 96-well pre-coated strip plates per kit (1 x NGF antibody, 1 x proNGF antibody coated plate), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Please refer to BEK-2212 (Human Mature NGF Rapid TM ELISA Kit) and BEK-2226 (Human proNGF Rapid TM ELISA Kit) .
Specificity:
Mature NGF ELISA: Detects human NGF, and cross-reacts with mouse and rat mature NGF. This mature NGF ELISA preferentially detects mature NGF over full-length proNGF. proNGF ELISA: Detects human proNGF only, does not cross-react with mouse and rat proNGF. Both capture and detection antibodies used in the proNGF ELISA kit binds to epitopes within the pro-domain of proNGF. Thus, the proNGF ELISA detects the full-length form of proNGF, and does not quantify mature NGF.
Typical limit of detection (LOD) for human mature NGF is 2 pg/mL determined as 150% of the blank value. Typical limit of detection (LOD) for human proNGF is < 60 pg/mL, determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
<b>Cross-reactivity of NGF isoforms:</b><br><br><b>Mature NGF ELISA:</b><br>The antibodies used in the Mature NGF ELISA kit bind epitopes within the mature domain of the protein. However, human proNGF protein shows <0.1% cross-reactivity (determined at 25 ng/mL, diluted in assay buffer), demonstrating the preferential quantification of mature NGF over full-length human proNGF. The absence of proNGF cross-reactivity in the mature NGF ELISA kit has been independently confirmed by <a class="newA" target="_blank" href="https://pubmed.ncbi.nlm.nih.gov/32870355/">Mossa AH <i>et al.</I> (2020)</a>. The Mature NGF ELISA does not cross-react with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect mature NGF from numerous other species including mouse and rat!<br><br><b>proNGF ELISA:</b><br>Does not cross-react with recombinant human mature NGF and proBDNF tested at 25 ng/mL. Does not react with rodent proNGF.
The Biosensis GDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and guinea pig serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. This GDNF ELISA kit employs a recombinant human GDNF standard obtained from the National Institute for Biological Standards and Control (NIBSC) and is therefore designed to accurately measure the human form of GDNF. Note that the antibodies used in this kit cross-react with guinea pig, rat and mouse GDNF. Guinea pig GDNF has been quantified in serum using the Human GDNF Rapid TM ELISA kit, since guinea pig GDNF protein shares closest amino acid sequence homology with human GDNF. Protein levels can be expressed as human GDNF equivalents. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Human recombinant GDNF with N-terminal methionine residue.
Applications:
ELISA
Application Details:
ELISA. For the quantification of Glial cell line-derived neurotrophic factor (GDNF) in Culture Supernatant, Cell Lysates, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plates, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Chen S et al. (2022) Newly Generated 3D Schwann-Like Cell Spheroids From Human Adipose-Derived Stem Cells Using a Modified Protocol. Cell Transplant. 31:9636897221093312. Application: Human cell culture supernatant. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine. Widbiller M et al. (2019) Neurotrophic Proteins in Dentin and Their Effect on Trigeminal Sensory Neurons. J Endod. [Epub ahead of print]. Application: Human tooth homogenate.
Specificity:
Human. The antibodies used in this kit detect guinea pig, mouse and rat GDNF. No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
Storage:
Store at 2-8°C.
Range:
7.8 - 500 pg/mL
Sample Type:
Cell Lysates,Culture Supernatant,Serum
Sensitivity:
Typical limit of detection (LOD) for rhGDNF is <5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
The Biosensis GDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and guinea pig serum only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. This GDNF ELISA kit employs a recombinant human GDNF standard obtained from the National Institute for Biological Standards and Control (NIBSC) and is therefore designed to accurately measure the human form of GDNF. Note that the antibodies used in this kit cross-react with guinea pig, rat and mouse GDNF. Guinea pig GDNF has been quantified in serum using the Human GDNF Rapid TM ELISA kit, since guinea pig GDNF protein shares closest amino acid sequence homology with human GDNF. Protein levels can be expressed as human GDNF equivalents. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Human recombinant GDNF with N-terminal methionine residue.
Applications:
ELISA
Application Details:
ELISA. For the quantification of Glial cell line-derived neurotrophic factor (GDNF) in Culture Supernatant, Cell Lysates, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Chen S et al. (2022) Newly Generated 3D Schwann-Like Cell Spheroids From Human Adipose-Derived Stem Cells Using a Modified Protocol. Cell Transplant. 31:9636897221093312. Application: Human cell culture supernatant. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine. Widbiller M et al. (2019) Neurotrophic Proteins in Dentin and Their Effect on Trigeminal Sensory Neurons. J Endod. [Epub ahead of print]. Application: Human tooth homogenate.
Specificity:
Human. The antibodies used in this kit detect guinea pig, mouse and rat GDNF. No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
Storage:
Store at 2-8°C.
Range:
7.8 - 500 pg/mL
Sample Type:
Cell Lysates,Culture Supernatant,Serum
Sensitivity:
Typical limit of detection (LOD) for rhGDNF is <5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
No cross-reactivity was observed for the following proteins tested at 25 ng/mL in assay buffer: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
The Biosensis NT3 Rapid TM enzyme-linked immune-sorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT3 in less than 4 hours in cell culture supernatants and human plasma (EDTA and citrate) only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human plasma. Accurate quantification of NT3 in human plasma requires a heterophilic antibody (HA) blocker which can be purchased separately ( BL-004-500 ). This ELISA kit consists of a pre-coated polyclonal anti-NT3 capture antibody, a biotinylated monoclonal anti-NT3 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product, which is directly proportional to the concentration of NT3 present in samples and protein standards. This NT3 Rapid TM ELISA kit employs a recombinant human NT3 standard. NT3 is highly conserved and nearly identical in many species. The assay's capture and detection antibodies detect NT3 from other species including mouse, rat and monkey, thus it is expected that this NT3 Rapid TM ELISA will also react with those species and possibly more because of the conversation of the immunogen used. The antibodies used in this kit bind to epitopes within the mature domain of NT3. Thus, this ELISA kit may detect the full-length pro-form of NT3. This kit has not been tested for other applications. Sufficient amount of NT3 standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
NT3 is understood to be important in promote the survival of visceral and proprioceptive sensory neurons. It is a secreted protein that belongs to the NGF-beta family and is found in brain and peripheral tissues.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Human recombinant NT3, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Neurotrophin-3 (NT-3) in Culture Supernatant, Plasma (Citrate), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plates, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Possamai-Della T et al. (2022) Imipramine Can Be Effective on Depressive-Like Behaviors, but Not on Neurotrophic Factor Levels in an Animal Model for Bipolar Disorder Induced by Ouabain Mol Neurobiol. [Epub ahead of print] Application: Rat, brain tissue homogenate. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine. Bavaresco DV et al. (2018) Depressive symptoms and neurotrophin levels in ostomy patients. J Bras Psiquiatr. 67(3). Application: Human serum. Shen W et al. (2017) Effects of Ranibizumab and Aflibercept on Human Mueller Cells and Photoreceptors under Stress Conditions. Int J Mol Sci. 2017 Mar 1;18(3). pii: E533. doi: 10.3390/ijms18030533. Application: Human cell line supernatants.
Specificity:
Human. The capture and detection antibodies used detect NT3 from other species including mouse, rat and monkey, thus it is expected that this NT3 ELISA will also react with those species.
The Biosensis NT3 Rapid TM enzyme-linked immune-sorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT3 in less than 4 hours in cell culture supernatants and human plasma (EDTA and citrate) only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human plasma. Accurate quantification of NT3 in human plasma requires a heterophilic antibody (HA) blocker which can be purchased separately ( BL-004-500 ). This ELISA kit consists of a pre-coated polyclonal anti-NT3 capture antibody, a biotinylated monoclonal anti-NT3 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product, which is directly proportional to the concentration of NT3 present in samples and protein standards. This NT3 Rapid TM ELISA kit employs a recombinant human NT3 standard. NT3 is highly conserved and nearly identical in many species. The assay's capture and detection antibodies detect NT3 from other species including mouse, rat and monkey, thus it is expected that this NT3 Rapid TM ELISA will also react with those species and possibly more because of the conversation of the immunogen used. The antibodies used in this kit bind to epitopes within the mature domain of NT3. Thus, this ELISA kit may detect the full-length pro-form of NT3. This kit has not been tested for other applications. Sufficient amount of NT3 standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
NT3 is understood to be important in promote the survival of visceral and proprioceptive sensory neurons. It is a secreted protein that belongs to the NGF-beta family and is found in brain and peripheral tissues.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Human recombinant NT3, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Neurotrophin-3 (NT-3) in Culture Supernatant, Plasma (Citrate), Plasma (EDTA). Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Possamai-Della T et al. (2022) Imipramine Can Be Effective on Depressive-Like Behaviors, but Not on Neurotrophic Factor Levels in an Animal Model for Bipolar Disorder Induced by Ouabain Mol Neurobiol. [Epub ahead of print] Application: Rat, brain tissue homogenate. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine. Bavaresco DV et al. (2018) Depressive symptoms and neurotrophin levels in ostomy patients. J Bras Psiquiatr. 67(3). Application: Human serum. Shen W et al. (2017) Effects of Ranibizumab and Aflibercept on Human Mueller Cells and Photoreceptors under Stress Conditions. Int J Mol Sci. 2017 Mar 1;18(3). pii: E533. doi: 10.3390/ijms18030533. Application: Human cell line supernatants.
Specificity:
Human. The capture and detection antibodies used detect NT3 from other species including mouse, rat and monkey, thus it is expected that this NT3 Rapid ELISA will also react with those species.
The Biosensis NGFR/p75 ECD Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mouse p75 ECD in less than 4 hours in cell culture supernatants and urine only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated mouse monoclonal anti-p75 ECD capture antibody, a goat anti- p75 ECD detection antibody and a horseradish peroxidase (HRP)-conjugated anti-goat antibody. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75ECD present in samples and protein standards. This NGFR/p75 ECD ELISA kit employs a recombinant mouse p75 ECD -Fc chimera protein as standard. While there is a current lack of a true mouse p75 ECD standard, this ELISA kit allows quantification of mouse p75 ECD as p75 ECD -Fc mouse equivalents. Please note that the antibodies used in this ELISA cross-react with human NGFR/p75 ECD . This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.
ELISA. For the quantification of Nerve growth factor receptor, extracellular domain (NGFR/p75ECD) in Culture Supernatant, Urine, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plates, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Luu BE et al. (2022) Modulation of diabetic kidney disease markers by an antagonist of p75NTR in streptozotocin-treated mice Gene. [Epub ahead of print]. Application: Mouse plasma. Zabbarova IV et al. (2018) Targeting p75 neurotrophin receptors ameliorates spinal cord injury-induced detrusor sphincter dyssynergia in mice. Neurourol Urodyn. 2018 May 28 [Epub ahead of print]. Application: Mouse urine. Maejima H et al. (2017) Exercise enhances cognitive function and neurotrophin expression in the hippocampus accompanied by changes in epigenetic programming in senescence-accelerated mice. Neurosci Lett. doi: 10.1016/j.neulet.2017.11.023. [Epub ahead of print]. Application: Mouse hippocampus homogenates.
Specificity:
Mouse. The antibodies used in this ELISA kit are known to cross-react with human p75ECD protein.
Storage:
Store at 2-8°C.
Range:
62.5 - 4,000 pg/mL
Sample Type:
Culture Supernatant,Tissue Homogenates,Urine
Sensitivity:
This ELISA kit typically detects 20-40 pg/mL of mouse p75ECD (defined as blank OD plus 3x the standard deviation of the blank OD, n=10).
The Biosensis NGFR/p75 ECD Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mouse p75 ECD in less than 4 hours in cell culture supernatants and urine only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated mouse monoclonal anti-p75 ECD capture antibody, a goat anti- p75 ECD detection antibody and a horseradish peroxidase (HRP)-conjugated anti-goat antibody. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75 ECD present in samples and protein standards. This NGFR/p75 ECD ELISA kit employs a recombinant mouse p75 ECD -Fc chimera protein as standard. While there is a current lack of a true mouse p75 ECD standard, this ELISA kit allows quantification of mouse p75 ECD as p75 ECD -Fc mouse equivalents. Please note that the antibodies used in this ELISA cross-react with human NGFR/p75 ECD . This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.
ELISA. For the quantification of Nerve growth factor receptor, extracellular domain (NGFR/p75ECD) in Culture Supernatant, Urine, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Luu BE et al. (2022) Modulation of diabetic kidney disease markers by an antagonist of p75NTR in streptozotocin-treated mice Gene. [Epub ahead of print]. Application: Mouse plasma. Zabbarova IV et al. (2018) Targeting p75 neurotrophin receptors ameliorates spinal cord injury-induced detrusor sphincter dyssynergia in mice. Neurourol Urodyn. 2018 May 28 [Epub ahead of print]. Application: Mouse urine. Maejima H et al. (2017) Exercise enhances cognitive function and neurotrophin expression in the hippocampus accompanied by changes in epigenetic programming in senescence-accelerated mice. Neurosci Lett. doi: 10.1016/j.neulet.2017.11.023. [Epub ahead of print]. Application: Mouse hippocampus homogenates.
Specificity:
Mouse. The antibodies used in this ELISA kit are known to cross-react with human p75ECD protein.
Storage:
Store at 2-8°C.
Range:
62.5 - 4,000 pg/mL
Sample Type:
Culture Supernatant,Tissue Homogenates,Urine
Sensitivity:
This ELISA kit typically detects 20-40 pg/mL of mouse p75ECD (defined as blank OD plus 3x the standard deviation of the blank OD, n=10).
The Biosensis Neurotrophin 4/5 Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT4/5 in less than 4 hours in cell culture supernatants, human citrate-plasma and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular blood samples. Accurate quantification of NT4/5 in human citrate-plasma requires a heterophilic antibody (HA) blocker which can be purchased separately ( BL-003-1000 ). This ELISA kit consists of a pre-coated polyclonal anti-NT4/5 capture antibody, a biotinylated anti-NT4/5 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product, which is directly proportional to the concentration of NT4/5 present in samples and protein standards. This NT4/5 ELISA kit employs a recombinant human NT4/5 standard. The capture and detection antibodies will also detect NT4/5 from other species due to a high degree of NT4/5 amino acid sequence homology. Therefore, this ELISA kit can be used to quantify NT4/5 in many species including mouse, rat and monkey. The antibodies used in this kit bind to epitopes within the mature domain of NT4/5. Thus, this ELISA kit will quantify the mature form of NT4/5, and may also detect the full-length pro-form of NT4/5. This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Primate,Rat
Immunogen:
Recombinant human NT4/5, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Neurotrophin-4/5 (NT-4/5) in Culture Supernatant, Plasma (Citrate), Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plates, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine. Ishimoto T et al. (2018) Ergothioneine-induced neuronal differentiation is mediated through activation of S6K1 and neurotrophin 4/5-TrkB signaling in murine neural stem cells. Cell Signal. [Epub ahead of print]. Application: Mouse cell lysate and brain homogenate. Allen RS et al. (2018) TrkB signaling pathway mediates the protective effects of exercise in the diabetic rat retina. Eur J Neurosci. doi: 10.1111/ejn.13909. [Epub ahead of print]. Application: Rat retina homogenates. Maejima H et al. (2017) Exercise enhances cognitive function and neurotrophin expression in the hippocampus accompanied by changes in epigenetic programming in senescence-accelerated mice. Neurosci Lett. doi: 10.1016/j.neulet.2017.11.023. [Epub ahead of print]. Application: Mouse hippocampus homogenates. Takahashi K et al. (2016) Exercise combined with low-level GABAA receptor inhibition up-regulates the expression of neurotrophins in the motor cortex. Neurosci Lett. doi: 10.1016/j.neulet.2016.10.052. [Epub ahead of print]. Application: Mouse cortex homogenates, in native cell lysis buffer.
Specificity:
Human. The capture and detection antibodies will also detect NT4/5 from other species due to a high degree of NT4/5 amino acid sequence homology. Therefore, this ELISA kit can be used to quantify NT4/5 in many species including mouse, rat and monkey.
The Biosensis Neurotrophin 4/5 Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT4/5 in less than 4 hours in cell culture supernatants, human citrate-plasma and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular blood samples. Accurate quantification of NT4/5 in human citrate-plasma requires a heterophilic antibody (HA) blocker which can be purchased separately ( BL-003-1000 ). This ELISA kit consists of a pre-coated polyclonal anti-NT4/5 capture antibody, a biotinylated anti-NT4/5 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product, which is directly proportional to the concentration of NT4/5 present in samples and protein standards. This NT4/5 ELISA kit employs a recombinant human NT4/5 standard. The capture and detection antibodies will also detect NT4/5 from other species due to a high degree of NT4/5 amino acid sequence homology. Therefore, this ELISA kit can be used to quantify NT4/5 in many species including mouse, rat and monkey. The antibodies used in this kit bind to epitopes within the mature domain of NT4/5. Thus, this ELISA kit will quantify the mature form of NT4/5, and may also detect the full-length pro-form of NT4/5. This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Primate,Rat
Immunogen:
Recombinant human NT4/5, made in E.coli
Applications:
ELISA
Application Details:
ELISA. For the quantification of Neurotrophin-4/5 (NT-4/5) in Culture Supernatant, Plasma (Citrate), Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine. Ishimoto T et al. (2018) Ergothioneine-induced neuronal differentiation is mediated through activation of S6K1 and neurotrophin 4/5-TrkB signaling in murine neural stem cells. Cell Signal. [Epub ahead of print]. Application: Mouse cell lysate and brain homogenate. Allen RS et al. (2018) TrkB signaling pathway mediates the protective effects of exercise in the diabetic rat retina. Eur J Neurosci. doi: 10.1111/ejn.13909. [Epub ahead of print]. Application: Rat retina homogenates. Maejima H et al. (2017) Exercise enhances cognitive function and neurotrophin expression in the hippocampus accompanied by changes in epigenetic programming in senescence-accelerated mice. Neurosci Lett. doi: 10.1016/j.neulet.2017.11.023. [Epub ahead of print]. Application: Mouse hippocampus homogenates. Takahashi K et al. (2016) Exercise combined with low-level GABAA receptor inhibition up-regulates the expression of neurotrophins in the motor cortex. Neurosci Lett. doi: 10.1016/j.neulet.2016.10.052. [Epub ahead of print]. Application: Mouse cortex homogenates, in native cell lysis buffer.
Specificity:
Human. The capture and detection antibodies will also detect NT4/5 from other species due to a high degree of NT4/5 amino acid sequence homology. Therefore, this ELISA kit can be used to quantify NT4/5 in many species including mouse, rat and monkey.
The Biosensis proBDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, cell lysates, serum, citrate-plasma and tissue extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples. This ELISA kit consists of a pre-coated polyclonal anti-proBDNF capture antibody, a biotinylated anti-matureBDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proBDNF present in samples and protein standards. A proBDNF positive control (QC sample) is provided to assure consistent assay performance. This proBDNF ELISA kit employs a recombinant, cleavage-resistant human proBDNF standard produced by Biosensis and validated against externally available proBDNF proteins. Due to a high degree of amino acid sequence homology, mouse and rat proBDNF can be quantified and expressed as human proBDNF equivalents. Internal Biosensis validation suggests that the use of the human standard provided in this kit will provide estimates that are identical, or close, to the actual levels of rat and mouse proBDNF present in rodent samples. Note that accurate proBDNF quantification in human serum and citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately . This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human proBDNF, mutated to be cleavage-resistant, made in 293F cells
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, pro- (proBDNF) in Culture Supernatant, Cell Lysates, Serum, Plasma (Citrate), Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plates, protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Aldhshan MS & Mizuno TM. (2022) "Effect of environmental enrichment on aggression and the expression of brain-derived neurotrophic factor transcript variants in group-housed male mice." Behav Brain Res. [Epub ahead of print]. Application: Brain tissue homogenate. Dorandish S et al. (2021) "Differences in the Relative Abundance of ProBDNF and Mature BDNF in A549 and H1299 Human Lung Cancer Cell Media." Int J Mol Sci. 22(13):7059. Application: Human culture supernatant. Payne AJ (2020) "The Effects of Alcohol on BDNF and CD5 Dependent Pathways." PhD Thesis. Application: Mouse RIPA tissue homogenates. Companys-Alemany J et al. (2020) "A Novel NMDA Receptor Antagonist Protects against Cognitive Decline Presented by Senescent Mice." Pharmaceutics. 12(3), 284. Application: Mouse hippocampal homogenates. Duart-Castells L et al. (2019) "7,8-dihydroxyflavone blocks the development of behavioral sensitization to MDPV, but not to cocaine: differential role of the BDNF-TrkB pathway." Biochem Pharmacol. [Epub ahead of print]. Application: Mouse RIPA tissue homogenates. Osborne A, Wang AX, Tassoni A, Widdowson PS, Martin KR (2018) "Design of a Novel Gene Therapy Construct to Achieve Sustained Brain-Derived Neurotrophic Factor Signalling in Neurons." Hum Gene Ther. [Epub ahead of print]. Application: Human cell line supernatant. Rahman MS, Millischer V, Zeebari Z, Forsell Y, Lavebratt C (2017) "BDNF Val66Met and childhood adversity on response to physical exercise and internet-based cognitive behavioural therapy in depressed Swedish adults." J Psychiatr Res. 93:50-58. Application: Human serum. Riffault B, Kourdougli N, Dumon C, Ferrand N, Buhler E, Schaller F, Chambon C, Rivera C, Gaiarsa JL, Porcher C (2016) "Pro-Brain-Derived Neurotrophic Factor (proBDNF)-Mediated p75NTR Activation Promotes Depolarizing Actions of GABA and Increases Susceptibility to Epileptic Seizures". Cereb. Cortex [Epub ahead of print]. Application: Rat cortex and hippocampus RIPA extracts. Hashimoto T, Shiina A, Hasegawa T, Kimura H, Oda Y, Niitsu T, Ishikawa M, Tachibana M, Muneoka K, Matsuki S, Nakazato M, Iyo M (2016) "Effect of mirtazapine versus selective serotonin reuptake inhibitors on benzodiazepine use in patients with major depressive disorder: a pragmatic, multicenter, open-label, randomized, active-controlled, 24-week trial." Ann Gen Psychiatry. 15(27). Application: Human serum. Niimi M, Hashimoto K, Kakuda W, Miyano S, Momosaki R, Ishima T, Abo M. (2016) "Role of Brain-Derived Neurotrophic Factor in Beneficial Effects of Repetitive Transcranial Magnetic Stimulation for Upper Limb Hemiparesis after Stroke." PLoS One. 11(3):e0152241. Application: Human serum. Stary CM, Sun X, Giffard RG (2015) "Astrocytes Protect against Isoflurane Neurotoxicity by Buffering pro-brain-derived Neurotrophic Factor." Anesthesiology. 123(4):810-9. Application: Rat neuron and astrocyte cell culture supernatant. Riffault B, Medina I, Dumon C, Thalman C, Ferrand N, Friedel P, Gaiarsa JL, Porcher C. (2014) "Pro-Brain-Derived Neurotrophic Factor Inhibits GABAergic Neurotransmission by Activating Endocytosis and Repression of GABAA Receptors." J. Neurosci. 34(40):13516-34. Application: Rat hippocampal culture supernatant.
Typical limit of detection (LOD) for proBDNF is 10 pg/mL determined as 150% of the blank value.
Cross Reactivity:
A cross-reactivity of 2% in weight concentration (0.9% in molar concentration) has been observed for mature BDNF assayed at 25 ng/mL (893 pmol/L) in Assay Diluent A.<br>Due to a high degree of sequence homology, this human proBDNF ELISA kit cross-reacts with the mouse and rat form of proBDNF. Other species have not yet been tested, but cross-reactivity with a wide range of mammalian forms of proBDNF is expected.<br> The antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
The Biosensis proBDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, cell lysates, serum, citrate-plasma and tissue extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples. This ELISA kit consists of a pre-coated polyclonal anti-proBDNF capture antibody, a biotinylated anti-matureBDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proBDNF present in samples and protein standards. A proBDNF positive control (QC sample) is provided to assure consistent assay performance. This proBDNF ELISA kit employs a recombinant, cleavage-resistant human proBDNF standard produced by Biosensis and validated against externally available proBDNF proteins. Due to a high degree of amino acid sequence homology, mouse and rat proBDNF can be quantified and expressed as human proBDNF equivalents. Internal Biosensis validation suggests that the use of the human standard provided in this kit will provide estimates that are identical, or close, to the actual levels of rat and mouse proBDNF present in rodent samples. Note that accurate proBDNF quantification in human serum and citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately . This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human proBDNF, mutated to be cleavage-resistant, made in 293F cells
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, pro- (proBDNF) in Culture Supernatant, Cell Lysates, Serum, Plasma (Citrate), Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate, protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Aldhshan MS & Mizuno TM. (2022) "Effect of environmental enrichment on aggression and the expression of brain-derived neurotrophic factor transcript variants in group-housed male mice." Behav Brain Res. [Epub ahead of print]. Application: Brain tissue homogenate. Dorandish S et al. (2021) "Differences in the Relative Abundance of ProBDNF and Mature BDNF in A549 and H1299 Human Lung Cancer Cell Media." Int J Mol Sci. 22(13):7059. Application: Human culture supernatant. Payne AJ (2020) "The Effects of Alcohol on BDNF and CD5 Dependent Pathways." PhD Thesis. Application: Mouse RIPA tissue homogenates. Companys-Alemany J et al. (2020) "A Novel NMDA Receptor Antagonist Protects against Cognitive Decline Presented by Senescent Mice." Pharmaceutics. 12(3), 284. Application: Mouse hippocampal homogenates. Duart-Castells L et al. (2019) "7,8-dihydroxyflavone blocks the development of behavioral sensitization to MDPV, but not to cocaine: differential role of the BDNF-TrkB pathway." Biochem Pharmacol. [Epub ahead of print]. Application: Mouse RIPA tissue homogenates. Osborne A, Wang AX, Tassoni A, Widdowson PS, Martin KR (2018) "Design of a Novel Gene Therapy Construct to Achieve Sustained Brain-Derived Neurotrophic Factor Signalling in Neurons." Hum Gene Ther. [Epub ahead of print]. Application: Human cell line supernatant. Rahman MS, Millischer V, Zeebari Z, Forsell Y, Lavebratt C (2017) "BDNF Val66Met and childhood adversity on response to physical exercise and internet-based cognitive behavioural therapy in depressed Swedish adults." J Psychiatr Res. 93:50-58. Application: Human serum. Riffault B, Kourdougli N, Dumon C, Ferrand N, Buhler E, Schaller F, Chambon C, Rivera C, Gaiarsa JL, Porcher C (2016) "Pro-Brain-Derived Neurotrophic Factor (proBDNF)-Mediated p75NTR Activation Promotes Depolarizing Actions of GABA and Increases Susceptibility to Epileptic Seizures". Cereb. Cortex [Epub ahead of print]. Application: Rat cortex and hippocampus RIPA extracts. Hashimoto T, Shiina A, Hasegawa T, Kimura H, Oda Y, Niitsu T, Ishikawa M, Tachibana M, Muneoka K, Matsuki S, Nakazato M, Iyo M (2016) "Effect of mirtazapine versus selective serotonin reuptake inhibitors on benzodiazepine use in patients with major depressive disorder: a pragmatic, multicenter, open-label, randomized, active-controlled, 24-week trial." Ann Gen Psychiatry. 15(27). Application: Human serum. Niimi M, Hashimoto K, Kakuda W, Miyano S, Momosaki R, Ishima T, Abo M. (2016) "Role of Brain-Derived Neurotrophic Factor in Beneficial Effects of Repetitive Transcranial Magnetic Stimulation for Upper Limb Hemiparesis after Stroke." PLoS One. 11(3):e0152241. Application: Human serum. Stary CM, Sun X, Giffard RG (2015) "Astrocytes Protect against Isoflurane Neurotoxicity by Buffering pro-brain-derived Neurotrophic Factor." Anesthesiology. 123(4):810-9. Application: Rat neuron and astrocyte cell culture supernatant. Riffault B, Medina I, Dumon C, Thalman C, Ferrand N, Friedel P, Gaiarsa JL, Porcher C. (2014) "Pro-Brain-Derived Neurotrophic Factor Inhibits GABAergic Neurotransmission by Activating Endocytosis and Repression of GABAA Receptors." J. Neurosci. 34(40):13516-34. Application: Rat hippocampal culture supernatant.
Typical limit of detection (LOD) for proBDNF is 10 pg/mL determined as 150% of the blank value.
Cross Reactivity:
A cross-reactivity of 2% in weight concentration (0.9% in molar concentration) has been observed for mature BDNF assayed at 25 ng/mL (893 pmol/L) in Assay Diluent A.<br>Due to a high degree of sequence homology, this human proBDNF ELISA kit cross-reacts with the mouse and rat form of proBDNF. Other species have not yet been tested, but cross-reactivity with a wide range of mammalian forms of proBDNF is expected.<br> The antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
The Biosensis Alpha-Synuclein Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of alpha-synuclein in less than 4 hours in human citrate-plasma, serum, CSF, as well as mouse and rat brain homogenates only if used as directed. Please refer to the kit protocol for specific use instructions for blood and CSF application. This ELISA kit consists of a pre-coated sheep polyclonal anti-alpha-synuclein (aa: 116-131) capture antibody, a biotinylated mouse monoclonal anti-alpha-synuclein detection antibody (aa: 61-95) and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of alpha-synuclein present in samples and protein standards. A human alpha-synuclein positive control (QC sample) is provided to assure consistent assay performance. This ELISA kit has not been tested for other substrate applications, only citrate plasma and CSF, other substrates have not been tested and performance may vary. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. Alpha synuclein has been associated with several neurodegenerative diseases. A point mutation in the gene coding for the alpha-synuclein protein was the first discovery linking this protein to a rare familial form of Parkinson's disease (PD). Subsequently, other mutations in the alpha-synuclein gene have been identified in familial PD. The aggregated proteinaceous inclusions called Lewy bodies found in PD and cortical Lewy body dementia (LBD) were discovered to be predominantly alpha-synuclein. Aberrant aggregation of alpha-synuclein has been detected in an increasing number of neurodegenerative diseases, collectively known as synucleopathies. Alpha-synuclein exists physiologically in both soluble and membrane-bound states, in unstructured and alpha-helical conformations, respectively. The physiological function of alpha-synuclein appears to require its translocation between these subcellular compartments and interconversion between the 2 conformations. Abnormal processing of alpha-synuclein is predicted to lead to pathological changes in its binding properties and function.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
The alpha-synuclein ELISA kit employs a recombinant human standard expressed in E.coli.
Applications:
ELISA
Application Details:
ELISA. For the quantification of alpha-synuclein in human serum, plasma (citrate), CSF, and mouse/rat tissue homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP; SNCA; PARK1;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate, protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Specificity:
Human, mouse and rat alpha-synuclein.
Storage:
Store at 2-8°C
Range:
0.16 - 10 ng/mL
Sample Type:
CSF,Plasma (Citrate),Serum,Tissue Homogenates
Sensitivity:
Typical limit of detection (LOD) for alpha-synuclein is < 100 pg/mL, determined as alpha-synuclein concentration at blank OD plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
Interference and cross-reactivity of human beta- and gamma-synuclein was assessed by spiking each protein at excess concentration of 20 ng/mL into two human plasma samples, each diluted 1:10 and 1:20, in comparison to unspiked samples. Cross-reactivity was calculated based on increase or decrease of apparent ?-synuclein concentrations in spiked samples. At a 3-6 fold excess (w/v) of spiked protein over endogenous alpha-synuclein at both sample dilutions, this ELISA shows very little cross-reactivity of 2.3% or less.<br>Individual alpha-synuclein isoforms have not yet been tested, but it is expected that this ELISA kit detects monomeric, oligomeric and phosphorylated alpha-synuclein isoforms.
The Biosensis Alpha-Synuclein Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of alpha-synuclein in less than 4 hours in human citrate-plasma, serum, CSF, as well as mouse and rat brain homogenates only if used as directed. Please refer to the kit protocol for specific use instructions for blood and CSF application. This ELISA kit consists of a pre-coated sheep polyclonal anti-alpha-synuclein (aa: 116-131) capture antibody, a biotinylated mouse monoclonal anti-alpha-synuclein detection antibody (aa: 61-95) and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of alpha-synuclein present in samples and protein standards. A human alpha-synuclein positive control (QC sample) is provided to assure consistent assay performance. This ELISA kit has not been tested for other substrate applications, only citrate plasma and CSF, other substrates have not been tested and performance may vary. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
Alpha synuclein is an abundant 140 amino acid neuronal protein, expressed primarily at presynaptic terminals in the central nervous system. Alpha synuclein has been associated with several neurodegenerative diseases. A point mutation in the gene coding for the alpha-synuclein protein was the first discovery linking this protein to a rare familial form of Parkinson's disease (PD). Subsequently, other mutations in the alpha-synuclein gene have been identified in familial PD. The aggregated proteinaceous inclusions called Lewy bodies found in PD and cortical Lewy body dementia (LBD) were discovered to be predominantly alpha-synuclein. Aberrant aggregation of alpha-synuclein has been detected in an increasing number of neurodegenerative diseases, collectively known as synucleopathies. Alpha-synuclein exists physiologically in both soluble and membrane-bound states, in unstructured and alpha-helical conformations, respectively. The physiological function of alpha-synuclein appears to require its translocation between these subcellular compartments and interconversion between the 2 conformations. Abnormal processing of alpha-synuclein is predicted to lead to pathological changes in its binding properties and function.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
The alpha-synuclein ELISA kit employs a recombinant human standard expressed in E.coli.
Applications:
ELISA
Application Details:
ELISA. For the quantification of alpha-synuclein in human serum, plasma (citrate), CSF, and mouse/rat tissue homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP; SNCA; PARK1;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate, protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Specificity:
Human, mouse and rat alpha-synuclein.
Storage:
Store at 2-8°C
Range:
0.16 - 10 ng/mL
Sample Type:
CSF,Plasma (Citrate),Serum,Tissue Homogenates
Sensitivity:
Typical limit of detection (LOD) for alpha-synuclein is < 100 pg/mL, determined as alpha-synuclein concentration at blank OD plus 3x standard deviation of blank OD (n=10).
Cross Reactivity:
Interference and cross-reactivity of human beta- and gamma-synuclein was assessed by spiking each protein at excess concentration of 20 ng/mL into two human plasma samples, each diluted 1:10 and 1:20, in comparison to unspiked samples. Cross-reactivity was calculated based on increase or decrease of apparent ?-synuclein concentrations in spiked samples. At a 3-6 fold excess (w/v) of spiked protein over endogenous alpha-synuclein at both sample dilutions, this ELISA shows very little cross-reactivity of 2.3% or less.<br>Individual alpha-synuclein isoforms have not yet been tested, but it is expected that this ELISA kit detects monomeric, oligomeric and phosphorylated alpha-synuclein isoforms.
The oligomeric form of Amyloid Beta peptide (A?, 1-42) has been closely linked to Alzheimer's Disease. Several ELISAs targeting A? have been developed; however, these ELISAs are known to cross-react with Amyloid Beta precursor protein (APP) and are poorly characterized against monomeric and oligomeric forms of the peptide. The Biosensis MOAB-2 antibody, developed by LaDu and co-workers (Youmans K. et al. , 2012) , has been shown to specifically detect A?, but not the precursor molecule APP. When utilized in ELISAs, the oligomeric form of A? peptide (o-A?) can be assayed independently of the other forms of the molecule when assayed with the MOAB-2 monoclonal antibody. The Biosensis oligomeric A? ELISA kit is a sandwich ELISA that allows the preferential quantification of oligomeric A? peptides. This kit is exclusive to Biosensis and consists of a pre-coated mouse monoclonal anti-A? capture antibody (MOAB-2), a biotinylated MOAB-2 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of o-A? present in samples and protein standards. The purpose of this kit is the in vitro qualitative measurement of oligomeric A? peptide levels in brain extracts and CSF samples from both transgenic mice and humans relative to a known o-A? standard. The inclusion of a highly validated oligomeric standard results in a unique, ready-to-use ELISA kit. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA Assay
Species Reactivity:
Human,Rat
Immunogen:
The standard in this ELISA is synthetically manufactured beta-amyloid peptide, amino acids 1-42 of human, HFIP treated and dried.The stabilized oligomeric beta amyloid 1-42 control complex is also constructed from the same synthetic peptide standard material. No animal systems were used for their manufacture.
Applications:
ELISA
Application Details:
ELISA. For the quantification of Oligomeric Amyloid-beta in CSF, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Kasus-Jacobi A et al. (2022) "Selecting Multitarget Peptides for Alzheimers Disease" Biomolecules. 12, 1386 Application: Human, A?142 oligomers. Eid A et al. (2022) "Effects of DDT on Amyloid Precursor Protein Levels and Amyloid Beta Pathology: Mechanistic Links to Alzheimer's Disease Risk" Environ Health Perspect. [Epub ahead of print] Application: Mouse, brain tissue homogenate. Kasus-Jacobi A et al. (2021) "Neutrophil Granule Proteins Inhibit Amyloid Beta Aggregation and Neurotoxicity." Curr Alzheimer Res. 18(5):414-427 Application: Mouse in-vitro assay, cell culture supernatant. Hark TJ et al. (2020) "Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer s Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals." Cell Syst. [Epub ahead of print] Application: Mouse cortical homogenates. Xiao L et al. (2020) "Enzyme-digested Colla Corii Asini (E'jiao) prevents hydrogen peroxide-induced cell death and accelerates amyloid beta clearance in neuronal-like PC12 cells." Neural Regen Res. 15(12): 2270-2 Application: Rat PC12 RIPA cell extract. Hrynchak MV et al. (2020) "Chronic Presence of Oligomeric A? Differentially Modulates Spine Parameters in the Hippocampus and Cortex of Mice With Low APP Transgene Expression." Front Synaptic Neurosci. Apr 24;12:16 Application: Mouse lysate. El-Sayed NA et al. (2019) "Design, synthesis, in vitro and in vivo evaluation of novel pyrrolizine-based compounds with potential activity as cholinesterase inhibitors and anti-Alzheimer's agents." Bioorg Chem. [Epub ahead of print] Application: Human. In-vitro screening of drug candidates. Oh Joo Kweon, Young Chul Youn, Yong Kwan Lim, Mi-Kyung Lee, Hye Ryoun Kim (2019) "Clinical utility of serum hepcidin and iron profile measurements in Alzheimer's disease." J Neurol Sci. [In press] Application: Human serum. Pacheco-Quinto J, Clausen D, Perez-Gonzalez R, Peng H, Meszaros A, Eckman CB, Levy E, Eckman EA (2018) "Intracellular metalloprotease activity controls intraneuronal A? aggregation and limits secretion of A? via exosomes." FASEB J. [Epub ahead of print] Application: Human cell line, mouse brain and organotypic brain slice cultures. Oh SB, Kim MS, Park S, Son H, Kim SY, Kim MS, Jo DG, Tak E, Lee JY (2018) "Clusterin contributes to early stage of Alzheimer's disease pathogenesis." Brain Pathol. [Epub ahead of print] Application: Transgenic mouse brain homogenates. S Liu, S Park, G Allington, F Prelli, Y Sun, M Marta-Ariza, H Scholtzova, G Biswas, B Brown, PB Verghese, PD Mehta, Y-U Kwon and T Wisniewski (2017) "Targeting Apolipoprotein E/Amyloid _ Binding by Peptoid CPO_A?17-21 P Ameliorates Alzheimer's Disease Related Pathology and Cognitive Decline." Sci Rep. 7(1):8009 Application: Transgenic mouse brain homogenates. M Cacciottolo, X Wang, I Driscoll, N Woodward, A Saffari, J Reyes, M L Serre, W Vizuete, C Sioutas, T E Morgan, M Gatz, H C Chui, S A Shumaker, S M Resnick, M A Espeland, C E Finch and J C Chen (2017) "Particulate air pollutants, APOE alleles and their contributions to cognitive impairment in older women and to amyloidogenesis in experimental models." Transl Psychiatry. Jan 31;7(1):e1022. Application: Extracts of E3FAD and E4FAD transgenic mouse brains. Riya Thomas, Paulina Zuchowska, Alan W. J. Morris, Felecia M. Marottoli, Sangeeta Sunny, Ryan Deaton, Peter H. Gann, Leon M. Tai (2016) "Epidermal growth factor prevents APOE4 and amyloid-beta-induced cognitive and cerebrovascular deficits in female mice." Acta Neuropathol Commun. 4(1):111 Application: Tris-extracts of EFAD transgenic mouse brains. Nor Faeizah Ibrahim, Daijiro Yanagisawa, Lina Wati Durani, Hamizah Shahirah Hamezah, Hanafi Ahmad Damanhuri, Wan Zurinah Wan Ngah, Mayumi Tsuji, Yuji Kiuchi, Kenjiro Ono, Ikuo Tooyama (2016) "Tocotrienol-Rich Fraction Modulates Amyloid Pathology and Improves Cognitive Function in A?PP/PS1 Mice." J Alzheimers Dis. [Epub ahead of print]. Application: Tris-extracts of mouse brain homogenates. Jia Luo, Sue H. Lee, Lawren VandeVrede, Zhihui Qin, Manel Ben Aissa, John Larson, Andrew F. Teich, Ottavio Arancio, Yohan D'Souza, Ahmed Elharram, Kevin Koster, Leon M. Tai, Mary Jo LaDu, Brian M. Bennett and Gregory R. J. Thatcher (2016) "A multifunctional therapeutic approach to disease modification in multiple familial mouse models and a novel sporadic model of Alzheimer's disease." Molecular Neurodegeneration 2016 11:35. Application: Tris-extracts of EFAD transgenic mouse brains. Weiguo Peng, Thiyagarajan M. Achariyar, Baoman Li, Yonghong Liao, Humberto Mestre, Emi Hitomi, Sean Regan, Tristan Kasper, Sisi Peng, Fengfei Ding, Helene Benveniste, Maiken Nedergaard, Rashid Dean (2016) "Suppression of glymphatic fluid transport in a mouse model of Alzheimer's disease." Neurobiology of Disease. Vol. 93, Pages 215-225 Application: TBSX-extracts of mouse cerebral cortex. Mafalda Cacciottolo, Amy Christensen, Alexandra Moser, Jiahui Liu, Christian J. Pike, Conor Smith, Mary Jo LaDu, Patrick M. Sullivan, Todd E. Morgan, Egor Dolzhenko, Andreas Charidimou, Lars-Olof Wahlund, Maria Kristofferson Wiberg, Sara Shams, Gloria Chia-Yi Chiang (2016) "The APOE4 allele shows opposite sex bias in microbleeds and Alzheimer's disease of humans and mice." Neurobiology of Aging. Volume 37, January 2016, Pages 47-57 Application: Tris-extracts of E3FAD and E4FAD transgenic mouse brains. Combes M, Poindron P, Callizot N.(2015) "Glutamate protects neuromuscular junctions from deleterious effects of ?-amyloid peptide and conversely: An in vitro study in a nerve-muscle coculture." J Neurosci. Res. 93(4):633-43 Application: Native Rat neurites & human muscle cell co-culture supernatants. Seo, Dong Han, et al. (2015) "Plasma-enabled sustainable elemental lifecycles: honeycomb-derived graphenes for next-generation biosensors and supercapacitors." Green Chem. 17:2164-2171. Application: Synthetic constructs. Tai, LM (2014) "Amyloid-_ Pathology and APOE Genotype Modulate Retinoid X Receptor Agonist Activity in vivo." J Biol Chem. 289(44):30538-55 Application: EFAD-Tg mice. Liu Y, Liu X, Hao W, Decker Y, Schomburg R, Fulop L, Pasparakis M, Menger MD, Fassbender K. (2014) "IKKbeta Deficiency in Myeloid Cells Ameliorates Alzheimer's Disease-Related Symptoms and Pathology." (2014) J Neurosci. Sep 24;34(39):12982-99 Application: Transgenic Mouse brain lysates, supernatants.
Specificity:
Human. MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to A? residues 1-4 and is highly specific just to amyloid beta peptide. The Biosensis o-A? Elisa detects A? oligomers as validated and described by Youmans KL et al (2012) and Rat by Combes M et al (2015). Rat.
The oligomeric form of Amyloid Beta peptide (A?, 1-42) has been closely linked to Alzheimer's Disease. Several ELISAs targeting A? have been developed; however, these ELISAs are known to cross-react with Amyloid Beta precursor protein (APP) and are poorly characterized against monomeric and oligomeric forms of the peptide. The Biosensis MOAB-2 antibody, developed by LaDu and co-workers (Youmans K. et al. , 2012) , has been shown to specifically detect A?, but not the precursor molecule APP. When utilized in ELISAs, the oligomeric form of A? peptide (o-A?) can be assayed independently of the other forms of the molecule when assayed with the MOAB-2 monoclonal antibody. The Biosensis oligomeric A? ELISA kit is a sandwich ELISA that allows the preferential quantification of oligomeric A? peptides. This kit is exclusive to Biosensis and consists of a pre-coated mouse monoclonal anti-A? capture antibody (MOAB-2), a biotinylated MOAB-2 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of o-A? present in samples and protein standards. The purpose of this kit is the in vitro qualitative measurement of oligomeric A? peptide levels in brain extracts and CSF samples from both transgenic mice and humans relative to a known o-A? standard. The inclusion of a highly validated oligomeric standard results in a unique, ready-to-use ELISA kit. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA Assay
Species Reactivity:
Human,Rat
Immunogen:
The standard in this ELISA is synthetically manufactured beta-amyloid peptide, amino acids 1-42 of human, HFIP treated and dried.The stabilized oligomeric beta amyloid 1-42 control complex is also constructed from the same synthetic peptide standard material. No animal systems were used for their manufacture.
Applications:
ELISA
Application Details:
ELISA. For the quantification of Oligomeric Amyloid-beta in CSF, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Kasus-Jacobi A et al. (2022) "Selecting Multitarget Peptides for Alzheimers Disease" Biomolecules. 12, 1386 Application: Human, A?142 oligomers. Eid A et al. (2022) "Effects of DDT on Amyloid Precursor Protein Levels and Amyloid Beta Pathology: Mechanistic Links to Alzheimer's Disease Risk" Environ Health Perspect. [Epub ahead of print] Application: Mouse, brain tissue homogenate. Kasus-Jacobi A et al. (2021) "Neutrophil Granule Proteins Inhibit Amyloid Beta Aggregation and Neurotoxicity." Curr Alzheimer Res. 18(5):414-427 Application: Mouse in-vitro assay, cell culture supernatant. Hark TJ et al. (2020) "Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer s Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals." Cell Syst. [Epub ahead of print] Application: Mouse cortical homogenates. Xiao L et al. (2020) "Enzyme-digested Colla Corii Asini (E'jiao) prevents hydrogen peroxide-induced cell death and accelerates amyloid beta clearance in neuronal-like PC12 cells." Neural Regen Res. 15(12): 2270-2 Application: Rat PC12 RIPA cell extract. Hrynchak MV et al. (2020) "Chronic Presence of Oligomeric A? Differentially Modulates Spine Parameters in the Hippocampus and Cortex of Mice With Low APP Transgene Expression." Front Synaptic Neurosci. Apr 24;12:16 Application: Mouse lysate. El-Sayed NA et al. (2019) "Design, synthesis, in vitro and in vivo evaluation of novel pyrrolizine-based compounds with potential activity as cholinesterase inhibitors and anti-Alzheimer's agents." Bioorg Chem. [Epub ahead of print] Application: Human. In-vitro screening of drug candidates. Oh Joo Kweon, Young Chul Youn, Yong Kwan Lim, Mi-Kyung Lee, Hye Ryoun Kim (2019) "Clinical utility of serum hepcidin and iron profile measurements in Alzheimer's disease." J Neurol Sci. [In press] Application: Human serum. Pacheco-Quinto J, Clausen D, Perez-Gonzalez R, Peng H, Meszaros A, Eckman CB, Levy E, Eckman EA (2018) "Intracellular metalloprotease activity controls intraneuronal A? aggregation and limits secretion of A? via exosomes." FASEB J. [Epub ahead of print] Application: Human cell line, mouse brain and organotypic brain slice cultures. Oh SB, Kim MS, Park S, Son H, Kim SY, Kim MS, Jo DG, Tak E, Lee JY (2018) "Clusterin contributes to early stage of Alzheimer's disease pathogenesis." Brain Pathol. [Epub ahead of print] Application: Transgenic mouse brain homogenates. S Liu, S Park, G Allington, F Prelli, Y Sun, M Marta-Ariza, H Scholtzova, G Biswas, B Brown, PB Verghese, PD Mehta, Y-U Kwon and T Wisniewski (2017) "Targeting Apolipoprotein E/Amyloid _ Binding by Peptoid CPO_A?17-21 P Ameliorates Alzheimer's Disease Related Pathology and Cognitive Decline." Sci Rep. 7(1):8009 Application: Transgenic mouse brain homogenates. M Cacciottolo, X Wang, I Driscoll, N Woodward, A Saffari, J Reyes, M L Serre, W Vizuete, C Sioutas, T E Morgan, M Gatz, H C Chui, S A Shumaker, S M Resnick, M A Espeland, C E Finch and J C Chen (2017) "Particulate air pollutants, APOE alleles and their contributions to cognitive impairment in older women and to amyloidogenesis in experimental models." Transl Psychiatry. Jan 31;7(1):e1022. Application: Extracts of E3FAD and E4FAD transgenic mouse brains. Riya Thomas, Paulina Zuchowska, Alan W. J. Morris, Felecia M. Marottoli, Sangeeta Sunny, Ryan Deaton, Peter H. Gann, Leon M. Tai (2016) "Epidermal growth factor prevents APOE4 and amyloid-beta-induced cognitive and cerebrovascular deficits in female mice." Acta Neuropathol Commun. 4(1):111 Application: Tris-extracts of EFAD transgenic mouse brains. Nor Faeizah Ibrahim, Daijiro Yanagisawa, Lina Wati Durani, Hamizah Shahirah Hamezah, Hanafi Ahmad Damanhuri, Wan Zurinah Wan Ngah, Mayumi Tsuji, Yuji Kiuchi, Kenjiro Ono, Ikuo Tooyama (2016) "Tocotrienol-Rich Fraction Modulates Amyloid Pathology and Improves Cognitive Function in A?PP/PS1 Mice." J Alzheimers Dis. [Epub ahead of print]. Application: Tris-extracts of mouse brain homogenates. Jia Luo, Sue H. Lee, Lawren VandeVrede, Zhihui Qin, Manel Ben Aissa, John Larson, Andrew F. Teich, Ottavio Arancio, Yohan D'Souza, Ahmed Elharram, Kevin Koster, Leon M. Tai, Mary Jo LaDu, Brian M. Bennett and Gregory R. J. Thatcher (2016) "A multifunctional therapeutic approach to disease modification in multiple familial mouse models and a novel sporadic model of Alzheimer's disease." Molecular Neurodegeneration 2016 11:35. Application: Tris-extracts of EFAD transgenic mouse brains. Weiguo Peng, Thiyagarajan M. Achariyar, Baoman Li, Yonghong Liao, Humberto Mestre, Emi Hitomi, Sean Regan, Tristan Kasper, Sisi Peng, Fengfei Ding, Helene Benveniste, Maiken Nedergaard, Rashid Dean (2016) "Suppression of glymphatic fluid transport in a mouse model of Alzheimer's disease." Neurobiology of Disease. Vol. 93, Pages 215-225 Application: TBSX-extracts of mouse cerebral cortex. Mafalda Cacciottolo, Amy Christensen, Alexandra Moser, Jiahui Liu, Christian J. Pike, Conor Smith, Mary Jo LaDu, Patrick M. Sullivan, Todd E. Morgan, Egor Dolzhenko, Andreas Charidimou, Lars-Olof Wahlund, Maria Kristofferson Wiberg, Sara Shams, Gloria Chia-Yi Chiang (2016) "The APOE4 allele shows opposite sex bias in microbleeds and Alzheimer's disease of humans and mice." Neurobiology of Aging. Volume 37, January 2016, Pages 47-57 Application: Tris-extracts of E3FAD and E4FAD transgenic mouse brains. Combes M, Poindron P, Callizot N.(2015) "Glutamate protects neuromuscular junctions from deleterious effects of ?-amyloid peptide and conversely: An in vitro study in a nerve-muscle coculture." J Neurosci. Res. 93(4):633-43 Application: Native Rat neurites & human muscle cell co-culture supernatants. Seo, Dong Han, et al. (2015) "Plasma-enabled sustainable elemental lifecycles: honeycomb-derived graphenes for next-generation biosensors and supercapacitors." Green Chem. 17:2164-2171. Application: Synthetic constructs. Tai, LM (2014) "Amyloid-_ Pathology and APOE Genotype Modulate Retinoid X Receptor Agonist Activity in vivo." J Biol Chem. 289(44):30538-55 Application: EFAD-Tg mice. Liu Y, Liu X, Hao W, Decker Y, Schomburg R, Fulop L, Pasparakis M, Menger MD, Fassbender K. (2014) "IKKbeta Deficiency in Myeloid Cells Ameliorates Alzheimer's Disease-Related Symptoms and Pathology." (2014) J Neurosci. Sep 24;34(39):12982-99 Application: Transgenic Mouse brain lysates, supernatants.
Specificity:
Human. MOAB-2 (mouse IgG2b) is a pan-specific, high-titer antibody to A? residues 1-4 and is highly specific just to amyloid beta peptide.The Biosensis o-A? Elisa detects A? oligomers as validated and described by Youmans KL et al (2012) and Rat by Combes M et al (2015). Rat.
The Biosensis NGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rat NGF in less than 3 hours in cell culture supernatants, serum, and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular serum samples. This ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of NGF present in samples and protein standards. This NGF ELISA kit is designed to measure rat NGF and thus employs a recombinant rat NGF protein. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and mouse! Guinea pig NGF has been quantified in serum using this ELISA kit using rat NGF protein as calibrator, as it shows largest sequence homology based on amino acid sequence among rodent and human NGF proteins. The assay antibodies preferentially detect the mature form of NGF, as shown by data comparing mature mouse NGF and mouse proNGF in the Mouse NGF ELISA kit (BEK-2213). Cross-reaction of full-length proNGF was This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by non-covalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Guinea Pig,Rat
Immunogen:
Sf 21 (baculovirus)-derived rat beta-NGF protein
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor (Beta-NGF) in Culture Supernatant, Serum, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Beta-nerve growth factor; Ngfb; NGF;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Mossa A et al. (2021) "Adaptation to partial urethral obstruction in healthy aging LOU rats and the role of nerve growth factor signaling pathway in the bladder." Exp Gerontol. [Epup ahead of print] Application: Rat urine. Itai S et al. (2020) "Cell-encapsulated chitosan-collagen hydrogel hybrid nerve guidance conduit for peripheral nerve regeneration." Biomed Microdevices. 22(4):81 Application: Rat cell culture supernatant. Cheppudira BP et al. (2016) "Anti-nerve growth factor antibody attenuates chronic morphine treatment-induced tolerance in the rat." BMC Anesthesiol. 16:73 Application: Acid-extracted rat spinal cord homogenate.
Storage:
Store at 2-8°C
Range:
3.9 - 250 pg/mL
Sample Type:
Culture Supernatant,Serum,Tissue Homogenates
Sensitivity:
Typical limit of detection (LOD) for rat NGF is 2 pg/mL determined as 150% of the blank value.
Cross Reactivity:
The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5 and proNGF protein tested at 25 ng/mL in assay buffer. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and mouse! <br><br>Mature NGF (27 kDa) and full-length proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the NGF ELISA calibration range (3.9-250 pg/mL; 0.14-9.2 pmol/L). OD readings for proNGF were indistinguishable from the assay's blank OD readings. Data was obtained with the Mouse NGF ELISA kit (BEK-2213) which uses the same assay capture and detection antibodies
The Biosensis NGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rat NGF in less than 3 hours in cell culture supernatants, serum, and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular serum samples. This ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of NGF present in samples and protein standards. This NGF ELISA kit is designed to measure rat NGF and thus employs a recombinant rat NGF protein. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and mouse! Guinea pig NGF has been quantified in serum using this ELISA kit using rat NGF protein as calibrator, as it shows largest sequence homology based on amino acid sequence among rodent and human NGF proteins. The assay antibodies preferentially detect the mature form of NGF, as shown by data comparing mature mouse NGF and mouse proNGF in the Mouse NGF ELISA kit (BEK-2213). Cross-reaction of full-length proNGF was This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by non-covalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Guinea Pig,Rat
Immunogen:
Sf 21 (baculovirus)-derived rat beta-NGF protein
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor (Beta-NGF) in Culture Supernatant, Serum, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Beta-nerve growth factor; Ngfb; NGF;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Mossa A et al. (2021) "Adaptation to partial urethral obstruction in healthy aging LOU rats and the role of nerve growth factor signaling pathway in the bladder." Exp Gerontol. [Epup ahead of print] Application: Rat urine. Itai S et al. (2020) "Cell-encapsulated chitosan-collagen hydrogel hybrid nerve guidance conduit for peripheral nerve regeneration." Biomed Microdevices. 22(4):81 Application: Rat cell culture supernatant. Cheppudira BP et al. (2016) "Anti-nerve growth factor antibody attenuates chronic morphine treatment-induced tolerance in the rat." BMC Anesthesiol. 16:73 Application: Acid-extracted rat spinal cord homogenate.
Storage:
Store at 2-8°C
Range:
3.9 - 250 pg/mL
Sample Type:
Culture Supernatant,Serum,Tissue Homogenates
Sensitivity:
Typical limit of detection (LOD) for rat NGF is 2 pg/mL determined as 150% of the blank value.
Cross Reactivity:
The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5 and proNGF protein tested at 25 ng/mL in assay buffer. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and mouse! <br><br>Mature NGF (27 kDa) and full-length proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the NGF ELISA calibration range (3.9-250 pg/mL; 0.14-9.2 pmol/L). OD readings for proNGF were indistinguishable from the assay's blank OD readings. Data was obtained with the Mouse NGF ELISA kit (BEK-2213) which uses the same assay capture and detection antibodies.
The Biosensis NGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mouse NGF in less than 3 hours in cell culture supernatants and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3,5,5-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of NGF present in samples and protein standards. A mouse NGF positive control (QC sample) is provided to assure consistent assay performance. This NGF ELISA kit is designed to measure mouse NGF and thus employs a mouse NGF standard. The mouse NGF standard supplied has been purified from mouse submaxillary glands according to published procedures. The calibrator standard reflects the native state of mouse NGF protein and has been chosen to give most accurate quantification of natural NGF protein levels in mouse samples. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and rat! This ELISA kit preferentially detects the mature form of NGF. Cross- reaction of full-length mouse proNGF was This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by non-covalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Mouse
Immunogen:
Native mouse NGF, purified from submaxillary glands
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor (Beta-NGF) in Culture Supernatant, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Beta-nerve growth factor; Ngfb; NGF;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Luu BE et al. (2022) Modulation of diabetic kidney disease markers by an antagonist of p75NTR in streptozotocin-treated mice Gene. [Epub ahead of print]. Application: Mouse kidney RIPA homogenates. Xu J et al. (2022) NGF-p75 signaling coordinates skeletal cell migration during bone repair Sci Adv. 8(11):eabl5716. Application: Mouse cell lysates. Nomura S et al. (2021) Effects of a Tea Cultivar "MK5601" on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice. J Nutr Sci Vitaminol (Tokyo). 67(3):170-179. Application: Mouse hippocampal RIPA homogenates. Sugimoto J et al. (2021) Fabry disease-associated globotriaosylceramide induces mechanical allodynia via activation of signaling through proNGF p75NTR but not mature NGF TrkA. Eur. J. Pharmacol. 895. Application: Mouse tissue homogenate (RIPA). La Porta C and Tappe-Theodor A (2020) Differential impact of psychological and psychophysical stress on low back pain in mice. Pain. 161(7):1442-1458. Application: Mouse tissue homogenate. Mossa AH et al. (2020) Antagonism of proNGF or its receptor p75 NTR reverses remodelling and improves bladder function in a mouse model of diabetic voiding dysfunction. Diabetologia. [Epub ahead of print]. Application: Mouse bladder extracts (RIPA). Roy S et al. (2020) Neurogenic Tissue Nanotransfection in the Management of Cutaneous Diabetic Polyneuropathy. Nanomedicine. [Epub ahead of print]. Application: Mouse lysate. Vigli D et al. (2018) Chronic treatment with the phytocannabinoid Cannabidivarin (CBDV) rescues behavioural alterations and brain atrophy in a mouse model of Rett syndrome. Neuropharmacology. 2018 Jul 26; [Epub ahead of print]. Application: Mouse hippocampus homogenate. Ryu JC et al. (2018). Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. [Epub ahead of print]. Application: Mouse urine.
Storage:
Store at 2-8°C
Range:
3.9 - 250 pg/mL
Sample Type:
Culture Supernatant,Tissue Homogenates
Sensitivity:
Typical limit of detection (LOD) for mouse NGF is 2 pg/mL determined as 150% of the blank value.
Cross Reactivity:
The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and NT-4/5 tested at 25 ng/mL in assay buffer. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and rat! <br><br>Mature mouse NGF (27 kDa) and full-length mouse proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the Mouse NGF ELISA calibration range (3.9-250 pg/mL; 0.14-9.2 pmol/L). OD readings for mouse proNGF were indistinguishable from the assay's blank OD readings.
The Biosensis NGF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mouse NGF in less than 3 hours in cell culture supernatants and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3,5,5-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of NGF present in samples and protein standards. A mouse NGF positive control (QC sample) is provided to assure consistent assay performance. This NGF ELISA kit is designed to measure mouse NGF and thus employs a mouse NGF standard. The mouse NGF standard supplied has been purified from mouse submaxillary glands according to published procedures. The calibrator standard reflects the native state of mouse NGF protein and has been chosen to give most accurate quantification of natural NGF protein levels in mouse samples. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and rat! This ELISA kit preferentially detects the mature form of NGF. Cross- reaction of full-length mouse proNGF was This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by non-covalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Mouse
Immunogen:
Native mouse NGF, purified from submaxillary glands
Applications:
ELISA
Application Details:
ELISA. For the quantification of Nerve growth factor (Beta-NGF) in Culture Supernatant, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Alternative Names:
Beta-nerve growth factor; Ngfb; NGF;
Biosensis Brand:
Rapid
Detection Method:
Colorimetric
Shelf Life:
12 months from purchase.
Use:
For research use only.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Luu BE et al. (2022) Modulation of diabetic kidney disease markers by an antagonist of p75NTR in streptozotocin-treated mice Gene. [Epub ahead of print]. Application: Mouse kidney RIPA homogenates. Xu J et al. (2022) NGF-p75 signaling coordinates skeletal cell migration during bone repair Sci Adv. 8(11):eabl5716. Application: Mouse cell lysates. Nomura S et al. (2021) Effects of a Tea Cultivar "MK5601" on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice. J Nutr Sci Vitaminol (Tokyo). 67(3):170-179. Application: Mouse hippocampal RIPA homogenates. Sugimoto J et al. (2021) Fabry disease-associated globotriaosylceramide induces mechanical allodynia via activation of signaling through proNGF p75NTR but not mature NGF TrkA. Eur. J. Pharmacol. 895. Application: Mouse tissue homogenate (RIPA). La Porta C and Tappe-Theodor A (2020) Differential impact of psychological and psychophysical stress on low back pain in mice. Pain. 161(7):1442-1458. Application: Mouse tissue homogenate. Mossa AH et al. (2020) Antagonism of proNGF or its receptor p75 NTR reverses remodelling and improves bladder function in a mouse model of diabetic voiding dysfunction. Diabetologia. [Epub ahead of print]. Application: Mouse bladder extracts (RIPA). Roy S et al. (2020) Neurogenic Tissue Nanotransfection in the Management of Cutaneous Diabetic Polyneuropathy. Nanomedicine. [Epub ahead of print]. Application: Mouse lysate. Vigli D et al. (2018) Chronic treatment with the phytocannabinoid Cannabidivarin (CBDV) rescues behavioural alterations and brain atrophy in a mouse model of Rett syndrome. Neuropharmacology. 2018 Jul 26; [Epub ahead of print]. Application: Mouse hippocampus homogenate. Ryu JC et al. (2018). Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. [Epub ahead of print]. Application: Mouse urine.
Storage:
Store at 2-8°C
Range:
3.9 - 250 pg/mL
Sample Type:
Culture Supernatant,Tissue Homogenates
Sensitivity:
Typical limit of detection (LOD) for mouse NGF is 2 pg/mL determined as 150% of the blank value.
Cross Reactivity:
The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and NT-4/5 tested at 25 ng/mL in assay buffer. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and rat! <br><br>Mature mouse NGF (27 kDa) and full-length mouse proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the Mouse NGF ELISA calibration range (3.9-250 pg/mL; 0.14-9.2 pmol/L). OD readings for mouse proNGF were indistinguishable from the assay's blank OD readings.
Target:
Nerve growth factor (Beta-NGF)
Uniprot Number:
P01139
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