This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. SOX10 is important and sensitive marker of melanoma especially for spindle cell and desmoplastic melanomas and schwannian neoplasms.
SOX2 is a transcription factor which is a member of SRY-related HMG-box (SOX) family. It has a role in the regulation of embryonic development and pluripotency of stem cells. It can be useful especially in lung squamous cell carcinoma diagnostic with panel of other relative markers of squamous carcinoma like P63/P40 and CK5/CK14 for example.
SOX2 is a transcription factor which is a member of SRY-related HMG-box (SOX) family. It has a role in the regulation of embryonic development and pluripotency of stem cells. It can be useful especially in lung squamous cell carcinoma diagnostic with panel of other relative markers of squamous carcinoma like P63/P40 and CK5/CK14 for example.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Transcription factor Sp1, also known as specificity protein 1* is a protein that in humans is encoded by the SP1 gene. The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. Subcellular Localization: Tissue Specificity:
STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
STAT1 peptide sequence 721-733 (DNLLPMSPEEFDE).
Applications:
IP,WB,IHC
Additional Info:
The antibody SM1 recognizes an epitope included within amino acids 721-733 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.
STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
STAT1 peptide sequence 8-23 (QLDSKFLEQVHQLYD) conjugated to KLH.
Applications:
IP,WB
Additional Info:
The antibody SM2 recognizes an epitope included within amino acids 8-23 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.
STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Synthetized peptide (C-terminal cytoplasmic part of STIM1).
Applications:
IP,WB,IHC,ICC
Additional Info:
The mouse monoclonal antibody CDN3H4 reacts with a cytoplasmic epitope of human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.
Clone number:
CDN3H4
Antibody Isotype:
IgG1
Application Details:
Immunocytochemistry: Methanol-aceton fixation; positive control: HeLa human cervix carcinoma cell line.Immunohistochemistry (paraffin sections): Recommended dilution: 5 ?g/ml.Western blotting: Recommended dilution: 1 ?g/ml; positive control: RBL rat basophilic leukemia cell line; both reducing and non-reducing conditions.
Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant fragment (aa 5-360) of human Syk.
Applications:
IP,WB,IHC,ICC
Additional Info:
The antibody SYK-01 reacts with protein tyrosine kinase p72Syk (Syk; an intracellular antigen), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FcepsilonRI).
Clone number:
SYK-01
Antibody Isotype:
IgG1
Application Details:
Western blotting: Recommended dilution: 1-2 ?g/ml; positive control: RBL rat basophilic leukemia cell line, A-431 human epidermoid carcinoma cell line, RAMOS lymphoma cell line, U-937 human histiocytic lymphoma cell line, JURKAT human peripheral blood T cell leukemia cell line; negative control: HeLa human cervix carcinoma cell line; non-reducing conditions. Immunohistochemistry (paraffin sections): Recommended dilution: 5 ?g/ml; positive tissue: tonsil B cells.
Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore. Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases. Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex.
Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore. Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases. Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex.
The p53 family of proteins includes three members, p53, p63, and p73. The protein p63 is encoded by TP63 gene, which gives rise to protein isoforms with different properties and functions due to the presence (TAp63) or absence (deltaNp63) of an N-terminal transactivation domain. Immunohistochemistry of p63 has a clinical relevance for certain tumor types, but investigations have been hampered by a lack of well characterized antibodies that are specific for p63, do not cross-react with the related p53 and p73 proteins, and allow for discrimination between p63 isoforms TAp63 and deltaNp63 with opposite functional properties.
The Mycobacterium tuberculosis antigen Tb10.3, also known as EsxR, ESAT-6 like protein 9 (ES6_9), or Rv3019c, is an almost uncharacterized conserved bacterial protein.
Product Type:
Antibodies Primary
Antibody Type:
polyclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant Tb10.3 protein (produced in E. coli)
Applications:
WB,ELISA
Additional Info:
The rabbit polyclonal antibody to Tb10.3 reacts with Mycobacterium tuberculosis protein Tb10.3 (EsxR).
The Mycobacterium tuberculosis antigen Tb10.4, also known as EsxH, Rv0288, ESAT-6 like protein EsxH, or Cfp7, is a conserved bacterial protein which effectively induces immune response to M. tuberculosis infection. It is a promissing vaccination tool.
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant Tb10.4 protein produced in E. coli
Applications:
WB,ELISA
Additional Info:
The rabbit polyclonal antibody to Tb10.4 reacts with Mycobacterium tuberculosis protein Tb10.4 (EsxH).
Mycobacterium tuberculosis protein Tb7.7, also known as Rv2654c, is being used for lymfocyte stimulation against Mycobacterium in combination with EsaT-6 and CFP-10 proteins.
Product Type:
Antibodies Primary
Antibody Type:
polyclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant Tb7.7 protein (produced in E. coli)
Applications:
WB,ELISA
Additional Info:
The rabbit polyclonal antibody to Tb7.7 reacts with the Mycobacterium tuberculosis protein Tb7.7 (Rv2654c).
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: 26S protease regulatory subunit 6A, also known as 26S proteasome AAA-ATPase subunit Rpt5, is an enzyme that in humans is encoded by the PSMC3 gene. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. Subcellular Localization: Tissue Specificity:
Tenascin C is an approximately 250 kDa extracellular matrix glycoprotein with important roles in the nervous system, as it promotes correct migration of growing axons during development and during neuronal regeneration. It is also involved in synaptic plasticity. Ligands of tenascin C are integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3, and alpha-V/beta-6. Similarly to neural cells, it also stimulates angiogenesis by promoting elongation and migration of endothelial cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Protein preparation from a homogenate of a human mammary tumour specimen.
Applications:
IHC,IP,WB
Additional Info:
The antibody T2H5 recognizes tenascin, a large hexameric extracellular matrix glycoprotein.
Clone number:
T2H5
Antibody Isotype:
IgG1
Application Details:
Immunohistochemistry (paraffin sections): Immunohistochemical detection of tenascin is valuable for studies of tissue differentiation and tumour growth. The antibody T2H5 is excellent for staining of paraffin-embedded tissue sections.Western blotting: Recommended dilution: 1-2 ?g/ml.
TFG (TRK-fused gene protein) is a regulatory protein with not fully understood function. Its defects are associated with various carcinomas, such as e.g. melanoma, thyroid papillary carcinoma, or glioma. TFG structure (multiple protein interaction motifs) indicated it can be an adaptor protein. It has been demonstrated TFG interacts with proteins modulating the NFkappaB pathway (TANK and NEMO). TNG enhances the effect of TNFalpha, TANK, TRAF2 and TRAF6 in inducing NFkappaB activity.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Human recombinant protein Trx-his-NTFG
Applications:
IP,WB,ICC
Additional Info:
The mouse monoclonal antibody TFG-03 recognizes TFG, an approximately 50 kDa intracellular protein with regulatory functions.
A synthetic peptide corresponding to a sequence at the N-terminus of human TGFBR2, different from the related mouse sequence by five amino acids, and from the related rat sequence by eight amino acids.
Store at -20?C for one year from date of receipt. After reconstitution, at 4?C for one month. It can also be aliquotted and stored frozen at -20?C for six months. Avoid repeated freeze-thaw cycles.Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Background: TGFBR2 (transforming growth factor, beta receptor II (70/80kDa)), also known as TGF-beta receptor type-2, TGFR-2, TGF-beta type II receptor, Transforming growth factor-beta receptor type II (TGF-beta receptor type II, TbetaR-II), is a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. A TGFBR2 cDNA encodes a deduced 565-amino acid protein with a calculated molecular mass of approximately 60 kD in length. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan syndrome, Loeys-Deitz aortic aneurysm syndrome, Osler-Weber-Rendu syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different informs have been characterized. Subcellular Localization: Tissue Specificity:
Store at -20?C for one year from date of receipt. After reconstitution, at 4?C for one month. It can also be aliquotted and stored frozen at -20?C for six months. Avoid repeated freeze-thaw cycles.Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Background: Thioredoxin, mitochondrial also known as thioredoxin-2 is a protein that in humans is encoded by the TXN2 gene on chromosome 22. It is mapped to 22q12.3. This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. Subcellular Localization: Mitochondrion. Tissue Specificity: Widely expressed in adult (at protein level) and fetal tissues.
Store at -20?C for one year from date of receipt. After reconstitution, at 4?C for one month. It can also be aliquotted and stored frozen at -20?C for six months. Avoid repeated freeze-thaw cycles.Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Background: Thioredoxin, mitochondrial also known as thioredoxin-2 is a protein that in humans is encoded by the TXN2 gene on chromosome 22. It is mapped to 22q12.3. This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. Subcellular Localization: Mitochondrion. Tissue Specificity: Widely expressed in adult (at protein level) and fetal tissues.
Thyroid disorders are often associated with autoimmune diseases and thyroid cancer.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
thyroid follicular cells
Applications:
IHC
Additional Info:
The antibody 2H11 recognizes thyroglobulin (TG), a 610 kDa extracellular secreted glycoprotein specific to the thyroid gland. TG is mainly expressed on thyroid follicular cells (99,1 %).
TIAR is an ubiquitously expressed RNA-binding protein, which regulates translational control, splicing, and other activities, including apoptosis. TIAR attenuates CDK1 activity, and is essential for the G2/M checkpoint. It accumulates in nuclear foci in late G2 phase and prophase in cells under replication stress. In steady state TIAR shuttles between the cytoplasm and the nucleus, probably as a part of nucleocytoplasmic transport of mRNA, but under stress conditions it accumulates mRNA molecules in granules and prevents their translation. Nucleolytic activity of TIAR against attacked target cells of cytotoxic lymphocytes has also been reported. Similarly, e.g. in permeabilized thymocytes TIAR triggers DNA fragmentation.
Store at -20?C for one year from date of receipt. After reconstitution, at 4?C for one month. It can also be aliquotted and stored frozen at -20?C for six months. Avoid repeated freeze-thaw cycles.Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Background: Tripartite motif-containing 33 (TRIM33), also known as transcriptional intermediary factor 1 gamma (TIF1-?), is a human gene. The TRIM33 gene is mapped to chromosome 1p13 by FISH. The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described; however, the full-length nature of one variant has not been determined. Subcellular Localization: Tissue Specificity:
Tissue non-specific alkaline phosphatase (TNAP), also known as liver/bone/kidney alkaline phosphatase, or MSCA-1 (mesenchymal stem cell antigen 1) is a selective marker for the prospective isolation of bone marrow-derived mesenchymal stem cells and mesenchymal stem-like cells. It is expressed at high levels in liver, bone, kidney, or endometrium, as well as on embryonic stem cells (ESCs). TNAP also plays a role in bone mineralization. Mutations in TNAP gene are associated with hypercalcemia and skeletal defects (hypophosphatasia).
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
WERI-RB-1 retinoblastoma cell line
Applications:
ICC,FC
Additional Info:
The mouse monoclonal antibody W8B2B10 recognizes TNAP (tissue non-specific alkaline phosphatase), an ectoenzyme expressed mainly on embryonic stem cells, liver, bone, and kidney cells. This antibody is suitable for characterization of bone marrow-derived MSCs, iPSCs, and ESCs.
Tissue non-specific alkaline phosphatase (TNAP), also known as liver/bone/kidney alkaline phosphatase, or MSCA-1 (mesenchymal stem cell antigen 1) is a selective marker for the prospective isolation of bone marrow-derived mesenchymal stem cells and mesenchymal stem-like cells. It is expressed at high levels in liver, bone, kidney, or endometrium, as well as on embryonic stem cells (ESCs). TNAP also plays a role in bone mineralization. Mutations in TNAP gene are associated with hypercalcemia and skeletal defects (hypophosphatasia).
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
WERI-RB-1 retinoblastoma cell line
Applications:
FC
Additional Info:
The mouse monoclonal antibody W8B2B10 recognizes TNAP (tissue non-specific alkaline phosphatase), an ectoenzyme expressed mainly on embryonic stem cells, liver, bone, and kidney cells. This antibody is suitable for characterization of bone marrow-derived MSCs, iPSCs, and ESCs.
Tissue non-specific alkaline phosphatase (TNAP), also known as liver/bone/kidney alkaline phosphatase, or MSCA-1 (mesenchymal stem cell antigen 1) is a selective marker for the prospective isolation of bone marrow-derived mesenchymal stem cells and mesenchymal stem-like cells. It is expressed at high levels in liver, bone, kidney, or endometrium, as well as on embryonic stem cells (ESCs). TNAP also plays a role in bone mineralization. Mutations in TNAP gene are associated with hypercalcemia and skeletal defects (hypophosphatasia).
Product Type:
Antibodies Primary
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
WERI-RB-1 retinoblastoma cell line
Applications:
FC
Additional Info:
The mouse monoclonal antibody W8B2B10 recognizes TNAP (tissue non-specific alkaline phosphatase), an ectoenzyme expressed mainly on embryonic stem cells, liver, bone, and kidney cells. This antibody is suitable for characterization of bone marrow-derived MSCs, iPSCs, and ESCs.
TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Recombinant human TNF-alpha
Applications:
FC
Additional Info:
The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
Clone number:
MAb11
Antibody Isotype:
IgG1 k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Recombinant human TNF-alpha
Applications:
FC
Additional Info:
The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
Clone number:
MAb11
Antibody Isotype:
IgG1 k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.
TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Recombinant human TNF-alpha
Applications:
FC
Additional Info:
The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
Clone number:
MAb11
Antibody Isotype:
IgG1 k
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant human TNF-alpha
Applications:
WB,ELISA
Additional Info:
The mouse monoclonal antibody MAb1 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
Clone number:
MAb1
Antibody Isotype:
IgG1 k
Application Details:
ELISA: It can be used as capture antibody in combination with biotinylated antibody MAb11.
TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant human TNF-alpha
Applications:
FC,IHC,ICC,ELISA
Additional Info:
The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
Clone number:
MAb11
Antibody Isotype:
IgG1 k
Application Details:
ELISA: Biotinylated MAb11 can be used as a detection antibody in combination with capture antibody MAb1. Immunohistochemistry (frozen sections): paraformaldehyde-fixed, saponin-treated frozen tissue sections. Flow cytometry: Intracellular staining.
TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant human TNF-alpha
Applications:
WB,ELISA,FA
Additional Info:
The mouse monoclonal antibody MAb1 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
Clone number:
MAb1
Antibody Isotype:
IgG1 k
Application Details:
Functional application: Neutralization. ELISA: It can be used as capture antibody in combination with biotinylated antibody MAb11.
TPX2 is a microtubule-associated protein, which is a critical regulator of mitosis. At the beginning of mitosis, TPX2 is released and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. After completion of mitosis the TPX2 protein disappears, but has also role in DNA damage response. Its overexpression has been demonstrated in many types of carcinomas. TPX2 belongs to the markers of worse tumor prognosis. On the other hand, down-regulation of TPX2 can inhibit cancer cell proliferation, migration and invasion.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Human TPX2
Applications:
IP,WB,IHC,ICC,ELISA
Additional Info:
The mouse monoclonal antibody TPX2-01 recognizes the epitope EPFVPKKEKKS (aa 636-646 in human) of TPX2, a microtubule-associated intracellular critical regulator of mitosis, which is overexpressed in many types of tumors and is a marker of worse prognosis in various cancers.
Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Purified porcine transferrin.
Applications:
WB,IHC,ICC,ELISA,RIA
Additional Info:
The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Purified porcine transferrin.
Applications:
WB,IHC,ICC,ELISA,RIA,FA
Additional Info:
The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
Clone number:
HTF-14
Antibody Isotype:
IgG1
Application Details:
Functional application: The antibody HTF-14 blocks binding of transferrin to its receptor. Immunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml; positive tissue: placenta. Western blotting: non-reducing conditions, recommended dilution: 1-2 ?g/ml.
Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Purified porcine transferrin.
Applications:
WB,IHC,ICC,ELISA,RIA,FA
Additional Info:
The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
Clone number:
HTF-14
Antibody Isotype:
IgG1
Application Details:
Functional application: The antibody HTF-14 blocks binding of transferrin to its receptor. Immunohistochemistry (paraffin sections): Recommended dilution: 10 ?g/ml; positive tissue: placenta. Western blotting: non-reducing conditions, recommended dilution: 1-2 ?g/ml.
Store at -20?C for one year from date of receipt. After reconstitution, at 4?C for one month. It can also be aliquotted and stored frozen at -20?C for six months. Avoid repeated freeze-thaw cycles.Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Background: Transketolase is a thiamine-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway. The pentose phosphate pathway, which is active in most tissues, provides sugar phosphates for intermediary biosynthesis, especially nucleotide metabolism, and generates the biosynthetic reducing power for the cell in the form of NADPH. Transketolase is directly involved in the branch of the pathway that channels excess sugar phosphates to glycolysis, enabling the production of NADPH to be maintained under different metabolic conditions. NADPH is critical for maintaining cerebral glutathione, and thus it is likely that transketolase plays an important role in brain metabolism. Subcellular Localization: Tissue Specificity:
Store at -20?C for one year from date of receipt. After reconstitution, at 4?C for one month. It can also be aliquotted and stored frozen at -20?C for six months. Avoid repeated freeze-thaw cycles.Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Background: Transketolase is a thiamine-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway. The pentose phosphate pathway, which is active in most tissues, provides sugar phosphates for intermediary biosynthesis, especially nucleotide metabolism, and generates the biosynthetic reducing power for the cell in the form of NADPH. Transketolase is directly involved in the branch of the pathway that channels excess sugar phosphates to glycolysis, enabling the production of NADPH to be maintained under different metabolic conditions. NADPH is critical for maintaining cerebral glutathione, and thus it is likely that transketolase plays an important role in brain metabolism. Subcellular Localization: Tissue Specificity:
TREM2 (Triggering receptor expressed on myeloid cells 2) is a cell surface transmembrane glycoprotein with an immunoglobulin like extracellular domain and a cytoplasmic tail [1]. It is expressed in myeloid cells including dendritic cells, granulocytes, and tissue-specific macrophages such as osteoclasts. In the brain, TREM2 is only expressed by microglia and in the central nervous system expression of TREM2 varies with higher expression in the hippocampus, spinal cord and white matter.
TREM2 plays a complex role in neuroinflammation with expression of TREM2 being upregulated in pathological conditions such as Parkinson's disease, Amyotrophic lateral sclerosis (ALS), stroke, traumatic brain injury and Alzheimer's Disease [2]. TREM2 has also been shown to have a role in potential mechanisms linking urban air pollution to Alzheimer's Disease through its involvement in the regulation of neuroinflammation [3].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
50 µg in 50 µl PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
Immunogen:
KLH conjugated synthetic peptide from the N-terminal region of human TREM2
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
TREM-2, TREM2a, TREM2b, TREM2c, Trggering receptor expressed on myeloid cells 2, Trggering receptor expressed on myeloid cells 2a, Triggering receptor expressed on monocytes 2
Research Areas:
Neuroscience
NS Reagents Product Area:
Neuroscience
Molecular Weight:
25kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Subcellular location:
Cell membrane, Secreted
Purification:
Affinity purification
References:
[1] Bouchon A, Dietrich J, Colonna M. Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes. J Immunol. 2000;164:49915.
[2] Gratuze, M., Leyns, C.E.G. & Holtzman, D.M. New insights into the role of TREM2 in Alzheimers disease. Mol Neurodegeneration 13, 66 (2018).
[3] Hendrik J. Greve, Christen L. Mumaw, Evan J. Messenger, Prasada R. S. Kodavanti, Joyce L. Royland, Urmila P. Kodavanti and Michelle L. Block. Diesel exhaust impairs TREM2 to dysregulate neuroinflammation. J Neuroinflammation. 2020; 17: 351.
TREM2 (Triggering receptor expressed on myeloid cells 2) is a cell surface transmembrane glycoprotein with an immunoglobulin like extracellular domain and a cytoplasmic tail [1]. It is expressed in myeloid cells including dendritic cells, granulocytes, and tissue-specific macrophages such as osteoclasts. In the brain, TREM2 is only expressed by microglia and in the central nervous system expression of TREM2 varies with higher expression in the hippocampus, spinal cord and white matter.
TREM2 plays a complex role in neuroinflammation with expression of TREM2 being upregulated in pathological conditions such as Parkinson's disease, Amyotrophic lateral sclerosis (ALS), stroke, traumatic brain injury and Alzheimer's Disease [2]. TREM2 has also been shown to have a role in potential mechanisms linking urban air pollution to Alzheimer's Disease through its involvement in the regulation of neuroinflammation [3].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
100 µg in 100 µl PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
Immunogen:
KLH conjugated synthetic peptide from the N-terminal region of human TREM2
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
TREM-2, TREM2a, TREM2b, TREM2c, Trggering receptor expressed on myeloid cells 2, Trggering receptor expressed on myeloid cells 2a, Triggering receptor expressed on monocytes 2
Research Areas:
Neuroscience
NS Reagents Product Area:
Neuroscience
Molecular Weight:
25kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Subcellular location:
Cell membrane, Secreted
Purification:
Affinity purification
References:
[1] Bouchon A, Dietrich J, Colonna M. Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes. J Immunol. 2000;164:49915.
[2] Gratuze, M., Leyns, C.E.G. & Holtzman, D.M. New insights into the role of TREM2 in Alzheimers disease. Mol Neurodegeneration 13, 66 (2018).
[3] Hendrik J. Greve, Christen L. Mumaw, Evan J. Messenger, Prasada R. S. Kodavanti, Joyce L. Royland, Urmila P. Kodavanti and Michelle L. Block. Diesel exhaust impairs TREM2 to dysregulate neuroinflammation. J Neuroinflammation. 2020; 17: 351.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: Tripartite motif-containing 24 (TRIM24) also known as transcriptional intermediary factor 1? (TIF1?) is a protein that, in humans, is encoded by the TRIM24 gene. The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Subcellular Localization: Tissue Specificity:
TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Recombinant intracellular domain (aa 29-186) of human TRIM.
Applications:
FC
Additional Info:
The antibody TRIM-04 recognizes an intracellular epitope of T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.
TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant intracellular domain (aa 29-186) of human TRIM.
Applications:
FC,IP,WB
Additional Info:
The antibody TRIM-04 recognizes an intracellular epitope of T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.
Clone number:
TRIM-04
Antibody Isotype:
IgG2a
Application Details:
Flow cytometry: Intracellular staining. Western blotting: Non-reducing conditions.
Thyroid transcription factor, also called thyroid specific enhancer binding nuclear protein (38 kDa), that regulates transcription activity of thyroid (thyroglobulin, thyroid peroxidase, sodium-iodide transport protein, calcitonin and MHC class I) and lung (surfactant proteins A, B and C, Clara cell secretory protein). The expression of TTF1 is confined to follicular epithelial cells and the C-cells in the thyroid, and to the type II pneumocytes and the Clara cells in the lung. In tumor diagnostics TTF1 distinguish primary (TTF+) vs. metastatic (usually TTF1-) lung carcinoma (LCa), pulmonary adenoca (TTF1+) from squamous cell Ca (usually TTF1 -), pleural lung Ca (TTF1+) vs.mesothelioma (TTF1 - ) and pulmonary small cell Ca (TTF1+) vs. Mercel cell Ca (TTF - ). Mucinous lung adeno ca is usually TTF1 negative.
Thyroid transcription factor, also called thyroid specific enhancer binding nuclear protein (38 kDa), that regulates transcription activity of thyroid (thyroglobulin, thyroid peroxidase, sodium-iodide transport protein, calcitonin and MHC class I) and lung (surfactant proteins A, B and C, Clara cell secretory protein). The expression of TTF1 is confined to follicular epithelial cells and the C-cells in the thyroid, and to the type II pneumocytes and the Clara cells in the lung. In tumor diagnostics TTF1 distinguish primary (TTF+) vs. metastatic (usually TTF1-) lung carcinoma (LCa), pulmonary adenoca (TTF1+) from squamous cell Ca (usually TTF1 -), pleural lung Ca (TTF1+) vs.mesothelioma (TTF1 - ) and pulmonary small cell Ca (TTF1+) vs. Mercel cell Ca (TTF - ). Mucinous lung adeno ca is usually TTF1 negative.
Store at -20?C for one year from date of receipt. After reconstitution, at 4?C for one month. It can also be aliquotted and stored frozen at -20?C for six months. Avoid repeated freeze-thaw cycles.Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Background: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blot studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, intellectual disability, and early-onset epilepsy caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Subcellular Localization: Tissue Specificity:
Store at -20?C for one year from date of receipt. After reconstitution, at 4?C for one month. It can also be aliquotted and stored frozen at -20?C for six months. Avoid repeated freeze-thaw cycles.Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Background: Splicing factor U2AF 65 kDa subunit is a protein that in humans is encoded by the U2AF2 gene. It is mapped to 19q13.42. U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. Subcellular Localization: Tissue Specificity:
Store at -20?C for one year from date of receipt. After reconstitution, at 4?C for one month. It can also be aliquotted and stored frozen at -20?C for six months. Avoid repeated freeze-thaw cycles.Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Background: Splicing factor U2AF 65 kDa subunit is a protein that in humans is encoded by the U2AF2 gene. It is mapped to 19q13.42. U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. Subcellular Localization: Tissue Specificity:
Ubinuclein 1 (UBN1) is a ubiquitously expressed evolutionarily conserved protein which binds to proliferation-promoting genes that are repressed by formation of senescence-associated heterochromatin foci (SAHF). Ubinuclein 1 associates with various transcription factors and with histone methyltransferase activity, is indispensable for SAHF formation and appears to be a regulator of senescence. Although in most cells ubinuclein is localized to the nucleus, in cells forming tight junctions it is recruited to the cell adhesion complexes, dependently on the cell density.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Recombinant protein corresponding to amino acids 1-190 of ubinuclein 1.
Applications:
IP,WB
Additional Info:
The antibody UBN1-02 recognizes N-terminal part of ubinuclein 1 (UBN1), a widely expressed nuclear and adhesion complex protein. Western blotting analysis reveals UBN1 as a 165 kDa band.
Clone number:
UBN1-02
Antibody Isotype:
IgG
Application Details:
Western blotting: Recommended dilution: 1-5 ?g/ml; positive control: HeLa cell line.
VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Applications:
FC
Additional Info:
The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.
Clone number:
Hs-14
Antibody Isotype:
IgM
Application Details:
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 ?l reagent / 100 ?l of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 50 tests. Intraacrosomal staining.
VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Applications:
FC,WB,ICC
Additional Info:
The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.
Clone number:
Hs-14
Antibody Isotype:
IgM
Application Details:
Immunocytochemistry: Recommended dilution: 10 ?g/ml, membrane permeabilization (acetone) is essential. The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought). Flow cytometry: Intraacrosomal staining; recommended dilution: 3-12 µg/ml.
Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Bacterially expressed full-length human vimentin
Applications:
FC
Additional Info:
The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament intracellular protein expressed on a wide variety of mesenchymal and mesodermal cell types.
Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Immunogen:
Bacterially expressed full-length human vimentin
Applications:
FC
Additional Info:
The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament intracellular protein expressed on a wide variety of mesenchymal and mesodermal cell types.
Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Applications:
IP,WB,IHC,ICC
Additional Info:
The antibody VI-10 reacts with vimentin, a 57 kDa intermediate filament intracellular protein expressed in variety of mesenchymal and mesodermal cell types.
Clone number:
VI-10
Antibody Isotype:
IgM
Application Details:
Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line. Immunohistochemistry (paraffin sections): Recommended dilution: 5 ?g/ml, positive tissue: skin fibroblast.Western blotting: Recommended dilution: 1-2 ?g/ml.
Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Pellet of porcine brain cold stable proteins after depolymerization of microtubules.
Applications:
IHC,WB,ICC
Additional Info:
The antibody VI-01 reacts with vimentin, a 57 kDa intermediate filament intracellular protein expressed in variety of mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscle desmin.
Clone number:
VI-01
Antibody Isotype:
IgM
Application Details:
Immunocytochemistry: Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line.
Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Bacterially expressed full-length human vimentin
Applications:
IHC,FC,WB,ICC,ELISA
Additional Info:
The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament intracellular protein expressed on a wide variety of mesenchymal and mesodermal cell types.
Clone number:
VI-RE/1
Antibody Isotype:
IgG1
Application Details:
ELISA: The antibody VI-RE/1 recognizes different epitope on human vimentin than the antibody VI-01 (IgM). Flow cytometry: Recommended dilution: 1-4 ?g/ml. Intracellular staining.Western blotting: Recommended dilution: 1-2 ?g/ml.Immunocytochemistry: Recommended dilution: 5-10 ?g/ml.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: WD repeat-containing protein 1 is a protein that in humans is encoded by the WDR1 gene. It is mapped to 4p16.1. This gene encodes a protein containing 9 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, mostly including a trp-asp at the C-terminal end. WD domains are involved in protein-protein interactions. The encoded protein may help induce the disassembly of actin filaments. Two transcript variants encoding different isoforms have been found for this gene. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: 14-3-3 protein epsilon is a protein that in humans is encoded by the YWHAE gene. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: YY1 (Yin Yang 1) is a transcriptional repressor protein in humans that is encoded by the YY1 gene. YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: YY1 (Yin Yang 1) is a transcriptional repressor protein in humans that is encoded by the YY1 gene. YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. Subcellular Localization: Tissue Specificity:
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. Background: YY1 (Yin Yang 1) is a transcriptional repressor protein in humans that is encoded by the YY1 gene. YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. Subcellular Localization: Tissue Specificity:
The ZAP70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP70 gene mutations resulting in lower ZAP70 protein expression levels or expression of catalytically inactive ZAP70 proteins, have been identified. ZAP70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency.In patients with chronic lymphocytic leukemia (B-CLL), ZAP70 expression on B cell was shown to be correlated with disease progression and survival. ZAP70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP70 on Tyr493 in the activation loop. ZAP70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.
Product Type:
Antibodies Primary
Antibody Type:
polyclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP70 with histidine tag
Applications:
WB
Additional Info:
The polyclonal antibody recognizes C-terminal part of human ZAP70 protein tyrosine kinase (intracellular antigen). ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
The ZAP70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP70 gene mutations resulting in lower ZAP70 protein expression levels or expression of catalytically inactive ZAP70 proteins, have been identified. ZAP70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency.In patients with chronic lymphocytic leukemia (B-CLL), ZAP70 expression on B cell was shown to be correlated with disease progression and survival. ZAP70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP70 on Tyr493 in the activation loop. ZAP70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP70 with histidine tag
Applications:
FC,WB,ICC
Additional Info:
The antibody ZAP-03 reacts with ZAP70, a 70 kDa protein tyrosine kinase expressed in T and NK cells (intracellular antigen). ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
Clone number:
ZAP-03
Antibody Isotype:
IgG1
Application Details:
Flow cytometry: Intracellular staining; recommended dilution: 2-5 ?g/ml; positive control: HPB-ALL human peripheral blood T cell leukemia cell line. Western blotting: Recommended dilution: 0,5 ?g/ml; positive control: HPB-ALL human peripheral blood T cell leukemia cell line; negative control: RAMOS human Burkitt lymphoma cell line; reducing conditions, 10% separating gel.
Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4 C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation, It will not affect the performance of the product.
Host Animal:
Rabbit
Species Reactivity:
Cucurbita sp.
Expected Species:
Glycne max, Oryza sativa Species of your interest not listed? Contact us
Immunogen:
ascorbate oxidase purified from Cucurbita sp.
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4 C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation, It will not affect the performance of the product.
Host Animal:
Rabbit
Species Reactivity:
Cucurbita sp.
Expected Species:
Glycne max, Oryza sativa Species of your interest not listed? Contact us
IgG concentration is 10 mg/ml, Biotin/IgG protein molar ratio is approximately 5,6, No foreign proteins are added
Application Details:
1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)
Purity:
Purified IgG in PBS pH 7.2.
Reconstitution:
For reconstitution add 1 ml of sterile water, Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution, If necessary, centrifuge to remove any particulates, Prepare fresh working dilution daily
Molecular Weight:
61 | 45 kDa (Cucurbita pepo)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography, N-Hydroxysuccinimidobiotin is used for labelling of antibody
L-ascorbate oxidase has oxidoreductase activity and belongs to multicopper oxidase family and is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.Alternative names: (ASO) (Ascorbase) (EC 1.10.3.3), AAO
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Cucurbita maxima
Expected Species:
Cucumis melo, Cucumis sativus, Nelumbo nucifera, Theobroma cacao Species of your interest not listed? Contact us
Reactivity of this antibody on endogenous material remains to be determined
Application Details:
1 : 1000 - 1: 5000 (WB)
Purity:
>95%, Protein G purified to a total immunoglobulin G fraction.
Molecular Weight:
65 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4Reactivity of this antibody on endogenous material remains to be determined.
Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
According to Konert et al. (2015) AOX antibody is recognizing AOX1A and AOX1D.This product can be sold containing ProClin if requested.
Application Details:
1 : 750 (IL), 1 : 1000 for 10-20 g of mitochondrial protein/lane detection (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
36-40 | 36-40 for Arabidopsis thaliana
Not reactive in:
Candidia albicans, Chlamydomonas reinhardtii (use an antibody to algal AOX1, AS06 152)
Selected references:
Brito et al. (2022) The role of the electron-transfer flavoprotein: ubiquinone oxidoreductase following carbohydrate starvation in Arabidopsis cell cultures. Plant Cell Rep. 2022 Jan 15. doi: 10.1007/s00299-021-02822-1. Epub ahead of print. PMID: 35031834.Pascual et al (2021). ACONITASE 3 is part of the ANAC017 transcription factor-dependent mitochondrial dysfunction response, Plant Physiology, 2021;, kiab225, https://doi.org/10.1093/plphys/kiab225Challabathula et al. (2021) Differential modulation of photosynthesis, ROS and antioxidant enzyme activities in stress-sensitive and -tolerant rice cultivars during salinity and drought upon restriction of COX and AOX pathways of mitochondrial oxidative electron transport, Journal of Plant Physiology,Volume 268,2022,153583, ISSN 0176-1617,https://doi.org/10.1016/j.jplph.2021.153583. Oh et al. (2021) Alternative oxidase (AOX) 1a and 1d limit proline-induced oxidative stress and aid salinity recovery in Arabidopsis. Plant Physiol. 2021 Dec 17:kiab578. doi: 10.1093/plphys/kiab578. Epub ahead of print. PMID: 34919733.Pavlovic & Kocab. (2021) Alternative oxidase (AOX) in the carnivorous pitcher plants of the genus Nepenthes: what is it good for? Ann Bot. 2021 Dec 18:mcab151. doi: 10.1093/aob/mcab151. Epub ahead of print. PMID: 34922341.
Special application note:
Mitochondrion inner membrane marker. Possibly in the inner surface of the inner mitochondrial membrane.Protocol for a plant mitochondria preparation can be found here. In protein samples which are older than few months AOX enzyme can undergo intensive dimerization. Such preparations should not be used to work with this antibody.
The mitochondrial AOX (alternative oxidase) of the unicellular green alga Chlamydomonas reinhardtii is encoded by two different genes, the Aox1 and Aox2. The alternative respiratory pathway is comprised of a single homodimeric protein – AOX – and functions as a mechanism to decrease the formation of reactive oxygen species (ROS) produced during respiratory electron transport. Alternative oxidase expression is influenced by different stress stimuli.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Aspergilus niger, Gonium pectorale, Monoraphidium neglectum, Nannochloropsis gaditana, Ostreococcus lucimarinus, Tetrabaena socialis, Volvox carteri f. nagariensis Species of your interest not listed? Contact us
Immunogen:
whole presumed mature AOX1 protein from from Chlamydomonas reinhardtii UniProt: O65000 fused to GST
Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022). https://doi.org/10.1038/s41586-022-04662-9Gu et al. (2021) A Lipid Bodies-Associated Galactosyl Hydrolase Is Involved in Triacylglycerol Biosynthesis and Galactolipid Turnover in the Unicellular Green Alga Chlamydomonas reinhardtiiPerlaza et al. (2019). The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response. Elife. 2019 Oct 15;8. pii: e49577. doi: 10.7554/eLife.49577.Kaye et al. (2019). The mitochondrial alternative oxidase from Chlamydomonas reinhardtii enables survival in high light.J Biol Chem. 2019 Jan 25;294(4):1380-1395. doi: 10.1074/jbc.RA118.004667.Zalutskaya et al. (2015). The Chlamydomonas reinhardtii alternative oxidase 1 is regulated by heat stress. Plant Physiol Biochem. 2015 Dec;97:229-34. doi: 10.1016/j.plaphy.2015.10.014.
Special application note:
Cellular [compartment marker] of Chlamydomonas reinhardtii mitochondrial inner membrane
Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water.
Product Type:
Antibody
Antibody Type:
Polyclonal
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AOX2 protein sequence, UniProt: O22049 , TAIR: At5g64210
FUNCTION: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. SUBUNIT: Adaptor protein complex 1 (AP-1) is an heterotetramer composed of two large adaptins (gamma1/AP1G1 or gamma2/AP1G2 and beta1A/AP1B1 or beta1B/AP1B1), a medium adaptin (mu1A/AP1M1 or mu1B/AP1M2) and a small adaptin (sigma1A/AP1S1 or sigma1B/AP1S2 or sigma1C/AP1S3). SUBCELLULAR LOCATION: Golgi apparatus. Cytoplasmic vesicle; cytoplasmic vesicle membrane; peripheral membrane protein; cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Widely expressed. DISEASE: Deletion of the AP1B1 gene may play a role in the tumorigenesis of meningiomas. SIMILARITY: Belongs to the adaptor complexes large subunit family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (C-KKGEIFELKAELNSDKKEK) corresponding to the amino acids 11-29 of human AP-1 complex subunit beta-1 conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from AP-1 complex subunit beta-1 protein in mouse, rat and bovine.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. This antibody works superbly in immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 1000 to 1: 5000 depending on tissue and detection method. For western blotting a dilution range of 1: 100 to 1: 10000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Adapter-related protein complex 1 beta-1 subunit; Beta-adaptin 1; Adaptor protein complex AP-1 beta-1 subunit; Golgi adaptor HA1/AP1 adaptin beta subunit; Clathrin assembly protein complex 1 beta large chain; AP1B1; ADTB1; BAM22
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody has been shown to be specific for AP-1 complex subunit beta-1. Rat and human, other species have not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans Golgi network to lysosomes. Together with medium and small subunits, adaptins form a heterotetrameric complex called an adaptor whose role is to promote the formation of clathrin-coated pits and vesicles and to recognise sorting signals within the cytosolic tails of transmembrane cargo molecules. Gamma-adaptin protein belongs to the adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network and/or endosomes. SUBCELLULAR LOCATION: Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane.
A synthetic peptide (IRLRELIRTIRTARTQAEER) corresponding to the amino acids 5-24 of human AP-1 complex subunit gamma-1 conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from AP-1 complex subunit gamma-1 protein in mouse, rat, dog and zebra fish.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. This antibody works in immunohistochemistry on frozen or wax embedded tissue. Antigen retrieval has been used in testing but may not be necessary. Typical working dilution: Light microscopy 1/500 to 1/2000 depending on tissue and detection method; Immunofluorescence 1/50 to 1/200; Western blotting 1/1000 to 1/4000 is recommended depending on detection method. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
AP-1 complex subunit gamma-1; Adapter-related protein complex 1 subunit gamma-1; Gamma1-adaptin; Adaptor protein complex AP-1 subunit gamma-1; Golgi adaptor HA1/AP1 adaptin subunit gamma-1; Clathrin assembly protein complex 1 gamma-1 large chain; AP1G1; ADTG; CLAPG1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody has been shown to be specific for AP-1 Rat and human
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent.
AP2 (floral homeotic protein APETALA 2) is a transcription factor involved in the specification of floral organ identity, establishment of floral meristem identity and controlling seed mass.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This antibody was used in western blots on Lepidium appelianum and Lepidium campestre AP2 Protein expressed in E. coli cells. Applied dilution was 1: 6000.Contains 0.02 % sodium azide.
Application Details:
1 : 1000-1 : 6000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
47,8 kDa
Not reactive in:
Nicotiana tabacum, Vitis vinifera
Selected references:
Wang et al. (2021). Brassinosteroids inhibit miRNA-mediated translational repression by decreasing AGO1 on the endoplasmic reticulum. J Integr Plant Biol. 2021 May 21. doi: 10.1111/jipb.13139. Epub ahead of print. PMID: 34020507.
Allophycocyanin is a protein of the light-harvesting phycobiliprotein family of red algae and cyanobacteria. It is an accessory pigment to chlorophyll A located in the core of the phycobilisome, and its strong spectral overlap with chlorophyll facilitates energy transfer for photosynthesis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Porphyridium cruentum, Synechocystis PCC 6803
Expected Species:
Red Algae, CyanobacteriaSpecies of your interest not listed? Contact us
Immunogen:
native allophycocyanin alpha and beta purified from Porphyridium cruentum phycobilisomes
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Ge at al. (2017). Translating Divergent Environmental Stresses into a Common Proteome Response through the Histidine Kinase 33 (Hik33) in a Model Cyanobacterium. Mol Cell Proteomics. 2017 Jul;16(7):1258-1274. doi: 10.1074/mcp.M116.068080.Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526.Gunnelius et al. (2014). The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. Nucleic Acids Res. 2014 Jan 29.Hernandez-Prieto et al. (2011). The small CAB-like proteins of the cyanobacterium Synechocystis sp. PCC 6803: Their involvement in chlorophyll biogenesis for Photosystem II. Bioch.Bioph. Acta.Gantt & Lipschultz (1974). Phycobilisome structure by immuno-electron microscopy. J. Phycology, Vol. 13:3, pages: 185-192. (immunolocalization)Gantt & Lipschultz (1974). Phycobilisomes of Porphyridium cruentum: Pigment Analysis. Biochem. 13:2960. Gantt E & C Lipschultz (1977). Probing phycobilisome structure by immuno-electron microscopy. J Phycol. 13:18
Special application note:
This product can be sold containing proclin if requested
Major allergen Api g 1, isoallergen 2 (Allergen Api g 1.0201) (allergen Api g 1)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Apium graveolens
Immunogen:
Recombinant Apium graveolens Major allergen Api g 1, isoallergen 2 protein, amino acids: 1-159, UniProt:P92918
Goat anti-Apolipoprotein E (ApoE) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Apolipoprotein E (ApoE) is a lipoprotein involved in fat metabolism and acts as cholesterol carrier between cells and across tissues. On a genetic level, three APOE alleles are described, APOE2, APOE3 and APOE4. These alleles give rise to six APOE isoforms, which are differentially implicated in various diseases. In the peripheral system, APOE4 is linked to increased risk of atherosclerosis. In the CNS, the ability of APOE4 in clearing beta-amyloid is impaired, while APOE3 and APOE2 are more efficient in performing this task. The APOE4 genotype in particular has been linked to increased risk for developing Alzheimer's Disease.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from a solution containing 50 mM Tris, pH 7.5, 0.4 M NaCl, 0.01 M EDTA, 3% trehalose, 0.07% sodium azide.
Host Animal:
Goat
Species Reactivity:
Human
Immunogen:
Recombinant human Apolipoprotein E
Applications:
ELISA
Antibody Isotype:
IgG
Application Details:
ELISA (0.1-1 µg/mL). Other applications not tested as yet. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
APOE;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human. Species cross-reactivity not tested.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution keep aliquots at -20°C to -80°C for higher stability. Avoid repetitive freeze/thaw cycles.
Biosensis is proud to offer the first commercially available ApoE/?-amyloid (ApoE/A?) complex ELISA kit. As a result of extensive collaboration with Dr. LaDu's laboratory at UIC and validation by Biosensis, this ELISA can be used to accurately and consistently measure the extent of ApoE/A? complex in tissue extracts and other samples. The Biosensis ApoE/A? Complex ELISA kit is a sandwich ELISA and consists of a pre-coated mouse monoclonal anti-A? capture antibody, a highly validated ApoE/A? complex standard that is pre-formed, lyophilized and ready for reconstitution, a biotinylated ApoE detection antibody, and horseradish peroxidase (HRP)-conjugated streptavidin and detection reagent. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the level of ApoE/A? complex present in samples and protein standards. Importantly, a well-characterized and unique ApoE/A? complex is included as a standard. This complex is pre-formed and lyophilized, requiring only reconstitution with assay diluent prior to use. In order to assess non-specific ApoE protein binding, each kit includes additional plates pre-coated with control antibody. The purpose of this kit is the in vitro qualitative measurement of ApoE/A? complexes in brain extracts and CSF samples from both transgenic mice and humans or primates, relative to a known ApoE/A? complex standard, only if used as directed. This kit has not been tested for other sample applications. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Complex of E.coli-derived recombinant human ApoE protein and synthetic, monomerized Abeta (1-42) peptide
Applications:
ELISA
Application Details:
ELISA. For the quantification of Apolipoprotein E/beta-Amyloid Complex (ApoE/A beta) in CSF, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 2 x 96-well pre-coated strip plates per 1 Plate Kit (1 plate MOAB-2 antibody coated, 1 plate control antibody coated), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Tai LM et al. (2013) J Biol Chem. 288(8): 5914-26 Tai LM et al. (2014) Mol Neurodegen. 9:2
Specificity:
Human Apolipoprotein E/beta-Amyloid (ApoE/A beta) Complex. The kit has been assayed on human samples only but the capture antibody, MOAB-2, is know to react with rodent amyloid beta though weaker (20% less reactivity on dot blots). The polyclonal APOE used for detection should detect ApoE from a variety of species but so far has only been tested on human
Biosensis is proud to offer the first commercially available ApoE/?-amyloid (ApoE/A?) complex ELISA kit. As a result of extensive collaboration with Dr. LaDu's laboratory at UIC and validation by Biosensis, this ELISA can be used to accurately and consistently measure the extent of ApoE/A? complex in tissue extracts and other samples. The Biosensis ApoE/A? Complex ELISA kit is a sandwich ELISA and consists of a pre-coated mouse monoclonal anti-A? capture antibody, a highly validated ApoE/A? complex standard that is pre-formed, lyophilized and ready for reconstitution, a biotinylated ApoE detection antibody, and horseradish peroxidase (HRP)-conjugated streptavidin and detection reagent. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the level of ApoE/A? complex present in samples and protein standards. Importantly, a well-characterized and unique ApoE/A? complex is included as a standard. This complex is pre-formed and lyophilized, requiring only reconstitution with assay diluent prior to use. In order to assess non-specific ApoE protein binding, each kit includes additional plates pre-coated with control antibody. The purpose of this kit is the in vitro qualitative measurement of ApoE/A? complexes in brain extracts and CSF samples from both transgenic mice and humans or primates, relative to a known ApoE/A? complex standard, only if used as directed. This kit has not been tested for other sample applications. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Complex of E.coli-derived recombinant human ApoE protein and synthetic, monomerized Abeta (1-42) peptide
Applications:
ELISA
Application Details:
ELISA. For the quantification of Apolipoprotein E/beta-Amyloid Complex (ApoE/A beta) in CSF, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 2 x 96-well pre-coated strip plates per 1 Plate Kit (1 plate MOAB-2 antibody coated, 1 plate control antibody coated), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Tai LM et al. (2013) J Biol Chem. 288(8): 5914-26 Tai LM et al. (2014) Mol Neurodegen. 9:2
Specificity:
Human Apolipoprotein E/beta-Amyloid (ApoE/A beta) Complex. The kit has been assayed on human samples only but the capture antibody, MOAB-2, is know to react with rodent amyloid beta though weaker (20% less reactivity on dot blots). The polyclonal APOE used for detection should detect ApoE from a variety of species but so far has only been tested on human
APP is an integral membrane protein found in any tissues and concentrated in the synapses of neurons. It is known as the precursor molecule generating amyloid beta (Aβ), and the amyloid fibrillar form is the primary component of amyloid plaques found in the brains of patients with Alzheimer's disease.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Human, mouse, rat
Expected Species:
Chicken, monkey and other species, please inquire.
Immunogen:
Synthetic peptide amino acids: 737-751 of human APP UniProt: P05067 or 85-99 of the C99 generated by secretases.
APP is an integral membrane protein found in many tissues and concentrated in the synapses of neurons. It is known as the precursor molecule generating amyloid beta (Aβ), and the amyloid fibrillar form is the primary component of amyloid plaques found in the brains of patients with Alzheimer's disease.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Human, mouse, rat
Immunogen:
Synthetic peptide (aa 653-662 of human APP) or 1-10 of the 4kDa Amyloid- peptide, The 4 kDa amyloid peptide is a 40 amino acid sequence that is cleaved of from the human amyloid A4 protein precursor (APP) and therefore the amino acids 1-10 of the peptide correspond to amino acids 653-662 of APP,
The Alzheimer Amyloid Precursor Protein (APP) is a transmembrane protein whose abnormal processing is associated with the pathogenesis of Alzheimer’s disease. APP695 lacking the protease inhibitor domain is the predominant form in neuronal tissues. APP695 is cleaved by caspases into the 664-residue amino (N)-terminal fragment that lacks the carboxyl C-terminal 31-residues (APPC31) and the 31-residues C-terminal fragment (APP-C31). Both fragments might be potent inducers of neuronal apoptosis. An antibody (named ACT1) against the N-terminus of caspase 3-generated APP C-terminal 31 aa of human APP695 (APP-C31 ) was raised in rabbit.
Product Type:
Antibody
Format:
Liquid
Storage Temp:
Store at -20 °C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Synthetic peptide corresponding to the N-terminal of human caspase 3-generated APP C-terminal 31 amino acids (aa 665-670) UniProt: P05067
Applications:
ELISA (ELISA), Immunolocalisation (IL), Western blot (WB)
Nishimura et al. (2002) Cell death induced by a caspase-cleaved transmembrane fragment of the Alzheimer amyloid precursor protein. Cell Death Differ. 2002 Feb;9(2):199-208. doi: 10.1038/sj.cdd.4400931. PMID: 11840170.
The Alzheimer amyloid precursor protein (APP) is a transmembrane protein whose abnormal processing is associated with the pathogenesis of Alzheimer’s disease. APP695 lacking the protease inhibitor domain is the predominant form in neuronal tissues. APP695 is cleaved by caspases into the 664-residue amino (N)-terminal fragment that lacks the carboxyl C-terminal 31-residues (APP delataC31) and the 31-residues C-terminal fragment (APP-C31). APP delta C31 potentially plays pathophysiological roles in neuronal death.
Product Type:
Antibody
Format:
Liquid
Storage Temp:
Store at -20 °C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Human, mouse, rat
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide corresponding to the C-terminal of the caspase 3-cleaved human APP (aa 658-664 of human APP695) UniProt: P05067
Applications:
ELISA (ELISA), Immunolocalisation (IL), Western blot (WB)
Nishimura et al (2003). Upregulation and antiapoptotic role of endogenous Alzheimer amyloid precursor protein in dorsal root ganglion neurons. Exp Cell Res. 2003 Jun 10;286(2):241-51. doi: 10.1016/s0014-4827(03)00066-1. PMID: 12749853.Nishimura et al. (2002) Cell death induced by a caspase-cleaved transmembrane fragment of the Alzheimer amyloid precursor protein. Cell Death Differ. 2002 Feb;9(2):199-208. doi: 10.1038/sj.cdd.4400931. PMID: 11840170.
Chicken anti-Appetite-regulating hormone, active (Active Ghrelin) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) and upon binding to the receptor it induces the release of growth hormone from the pituitary. This ligand has an appetite-stimulating effect and is involved in growth regulation (Ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Human active Ghrelin peptide (24-33 aa) S3n-octanoicacid covalently linked to amino acid 28
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA . Suggested dilution at 1:500 to 1:4,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Appetite-regulating hormone (Grehlin) Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) and upon binding to the receptor it induces the release of growth hormone from the pituitary. This ligand has an appetite-stimulating effect and is involved in growth regulation (Ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic peptide from human Ghrelin, C-terminal, (17-28 aa)
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA. Suggested dilution at 1:500 to 1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
APX plays a key role in plant antioxidant system by reducing hydrogen peroxide to water.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Coccomyxa subellipsoidea C-169,yanidioschyzon merolae, Micromonas pusilla (strain CCMP1545), Nannochloropsis gaditana, Ostreococcus lucimarinus (strain CCE9901), Ulva fasciata, Volvox carteriSpecies of your interest not listed? Contact us
APX plays a key role in plant antioxidant system by reducing hydrogen peroxide to water. Cellular localization includes chloroplast (tAPX and sAPX), cytosol (cAPX) and peroxisome (pAPX).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Brassica rapa subsp. oleifera Stromal APX; Glycine max, Glycine soja L-ascorbate peroxidase T, chloroplastic; Medicago truncatula thylakoid-bound APX; Mesembryanthemum crystallinum, Pisum sativum Chloroplast stromal ascorbate peroxidase 12; Solanum lycopersicum thylakoid-bound APX; Spinacia oleracea stromal APX; Theobroma cacao L-APX T isoform 3; Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
BSA-conjugated synthetic peptide derived from Arabidopsis thaliana tAPX (thylakoidal ascorbate peroxidase) UniProt: Q42593-1, TAIR:At1g77490 and sAPX (stromal/mitochondrial ascorbate peroxidase) UniProt: Q42592-1 TAIR: At4g08390 Five out of twelve amino acids are also identical with cAPX1 (At1g07890), cAPX2 (At3g09640) and pAPX (At4g35000)
This product can be sold containing proclin if requested
Application Details:
1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
25-38 kDa for A. thaliana
Not reactive in:
Algae, Helianthus annus, Marchantia polymorpha
Selected references:
Wang et al. (2022) Reciprocity between a retrograde signal and a putative metalloprotease reconfigures plastidial metabolic and structural states. Sci Adv. 2022 Jun 3;8(22):eabo0724. doi: 10.1126/sciadv.abo0724. Epub 2022 Jun 3. PMID: 35658042; PMCID: PMC9166295.Kucko et al. (2022) The acceleration of yellow lupine flower abscission by jasmonates is accompanied by lipid-related events in abscission zone cells, Plant Science, Volume 316, 2022,111173, ISSN 0168-9452, https://doi.org/10.1016/j.plantsci.2021.111173. (https://www.sciencedirect.com/science/article/pii/S0168945221003691)Jedelska et al. (2021) Protein S-nitrosation differentially modulates tomato responses to infection by hemi-biotrophic oomycetes of Phytophthora spp. Hortic Res. 2021 Feb 1;8(1):34. doi: 10.1038/s41438-021-00469-3. PMID: 33518717; PMCID: PMC7848004.Tokarz et al. (2021). Stem Photosynthesis-A Key Element of Grass Pea (Lathyrus sativus L.) Acclimatisation to Salinity. Int J Mol Sci. 2021 Jan 12;22(2):685. doi: 10.3390/ijms22020685. PMID: 33445673; PMCID: PMC7828162.Tokarz et al. (2020). Can Ceylon Leadwort ( Plumbago zeylanica L.) Acclimate to Lead Toxicity?-Studies of Photosynthetic Apparatus Efficiency. Int J Mol Sci. 2020 Mar 9;21(5):1866.doi: 10.3390/ijms21051866.
Arabinogalactans are polymers composed of arabinose and galactose monosaccharides. They exist in plants as free glycans or are attached to rhamnogalacturonan I or to protetein backbones. When attached to proteins they form arabinogalactan protein (AGP) which works as an intercellular signaling molecule. AGP also functions as a glue for sealing wounds in plants. Arabinogalactans can be used as an additive in food, and as a replacement for starch in food or pharmaceutical products.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Antibody can be stored up to 1 month at 4 C, and at -80°Cfor up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Arabinogalactans are polymers composed of arabinose and galactose monosaccharides. They exist in plants as free glycans or are attached to rhamnogalacturonan I or to protetein backbones. When attached to proteins they form arabinogalactan protein (AGP) which works as an intercellular signaling molecule. AGP also functions as a glue for sealing wounds in plants. Arabinogalactans can be used as an additive in food, and as a replacement for starch in food or pharmaceutical products. This pectic polysaccharide antibody belongs to the arabinogalactan 3 group of antibodies. Pectin consists of polysaccharides found in the primary cell wall of most plants which binds cells together in the middle lamella.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Antibody can be stored up to 1 month at 4 C, and at -80°Cfor up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Arabinogalactans are polymers composed of arabinose and galactose monosaccharides. They exist in plants as free glycans or are attached to rhamnogalacturonan I or to protetein backbones. When attached to proteins they form arabinogalactan protein (AGP) which works as an intercellular signaling molecule. AGP also functions as a glue for sealing wounds in plants. Arabinogalactans can be used as an additive in food, and as a replacement for starch in food or pharmaceutical products. This pectic polysaccharide antibody belongs to the arabinogalactan-4 group of antibodies. Pectin consists of polysaccharides found in the primary cell wall of most plants which binds cells together in the middle lamella.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Antibody can be stored up to 1 month at 4 C, and at -80°Cfor up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C. Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rat
Species Reactivity:
Higher plants
Immunogen:
Polysaccharide Arabinogalactan-protein (AGP) from Oryza sativa
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C. Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rat
Species Reactivity:
Higher plants
Immunogen:
Polysaccharide Arabinogalactan-protein (AGP) from Oryza sativa
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C. Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rat
Species Reactivity:
Higher plants
Immunogen:
Polysaccharide Arabinogalactan-protein (AGP) from Oryza sativa
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C. Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rat
Species Reactivity:
Higher plants
Immunogen:
Polysaccharide Arabinogalactan-protein (AGP) from Oryza sativa
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C. Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rat
Species Reactivity:
Higher plants
Immunogen:
Polysaccharide Arabinogalactan-protein (AGP) from Oryza sativa
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at +4°C(short term) and at -20 °C (long term).
Host Animal:
Rat
Species Reactivity:
Higher plants, ferns and mosses
Immunogen:
Polysaccharide Arabinogalactan-protein (AGP) from Oryza sativa
Antibody is recognizing carbohydrate epitope containing Β-linked glucuronic acid.
Application Details:
1:10 (ELISA, IF)
Conjugation:
IgM
Isotype:
IgM
Purity:
Cell culture supernatant.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Stacey et al. (1990). Patterns of expression of the JIM4 arabinogalactan-protein epitope in cell cultures and during somatic embryogenesis in Daucus carota L Planta. 1990 Jan;180(2):285-92.doi: 10.1007/BF00194009. Knox et al.(1991). Developmentally regulated epitopes of cell surface arabinogalactan proteins and their relation to root tissue pattern formation. Plant J. 1991 ov;1(3):317-326.doi: 10.1046/j.1365-313X.1991.t01-9-00999.x.
Special application note:
Contains 0.05% Sodium Azide.This antibody is made to rice arabinogalactan-proteins (AGPs) and it recognizes a carbohydrate epitope containing B-linked glucuronic acid. In competitive inhibition ELISAs antibody binding to gum arabic was inhibited (50%) by 70 mg/ml 1-O-methyl-B-D-GlcA. The binding of the antibody to AGPs can be fully inhibited by 10 mM 1-O-methyl-B-D-GlcA.
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C. Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rat
Species Reactivity:
Higher plants
Immunogen:
Polysaccharide Arabinogalactan-protein (AGP) from Oryza sativa
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C. Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rat
Species Reactivity:
Higher plants
Immunogen:
Polysaccharide Arabinogalactan-protein (AGP) from Oryza sativa
Peanut Ara h1 is a major peanut allergen. It shows significant homology with the vicilin seed storage protein found in most higher plants.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arachis hypogaea
Immunogen:
Recombinant peanut allergen Ara h1, UniProt: P43237, amino acid 26-216.
Arachin Ahy-3 is a hexamer, composed of an acidic and a basic chain, derived from a single precursor and linked by a disulfied bond. Belongs to the 11S seed storage protein (globulins) family. Arachin Ahy-3 chain alpha; Arachin Ahy-3 chain beta.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Conglutin (allergen Ara h 6) causes allergic reaction in humans and binds to IgE.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C or -80 C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
ARC5 (Dynamin-like protein ARC5) is a probable GTPase component of both plastid and peroxisme division machinery. Required for the last steps of plastid division specifically in mesophyll-cell, when the narrow isthmus breaks, facilitating the separation of the daughter plastids. Necessary for peroxisome activities. Seems to influence stromule (stroma-filled tubular extensions of the plastid envelope membrane) length and frequency. Alternative names: Dynamin-related protein 5B Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Shelf life upon re-constitution is 6 months. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Store reconstituted antibodies at 4°Cor in -20 for extrended periods of time. Lyophilized powder is stable for a minimum of 2 years at -20 °C.
Host Animal:
Rabbit
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from internal domain of Dynamin-like protein ARC5 from Arabidopsis thaliana Uniprot: Q84N64,TAIR: AT3G19720
Antibody format is a total IgG (purified on Protein A)
Application Details:
1 : 500-1 : 5 000 (WB)
Purity:
Total IgG in Tris 0,1M, glycine 0,1M, sucrose 2%.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
87 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Pipitone et al. (2021). A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis. Elife. 2021 Feb 25;10:e62709. doi: 10.7554/eLife.62709. PMID: 33629953; PMCID: PMC7906606.
The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It is a small GTPase that undergoes a GDP/GTP nucleotide exchange cycle and it is an important regulator of cellular trafficking.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
References describing immunolocalization (IF) and (IG) studies:Pimpl et al (2000). In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles. Plant Cell. 2000 Nov;12(11):2219-36.Ritzenthaler et al. (2002). Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera. Plant Cell. 2002 Jan;14(1):237-61.
Application Details:
1 : 1000 (IF), 1 : 100 (IG), 1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
21 kDa (Arabidopsis thaliana)
Not reactive in:
Microsporidia sp.
Selected references:
Farago et al. (2022) Small paraquat resistance proteins modulate paraquat and ABA responses and confer drought tolerance to overexpressing Arabidopsis plants. Plant Cell Environ. 2022 Jul;45(7):1985-2003. doi: 10.1111/pce.14338. Epub 2022 Apr 29. PMID: 35486392; PMCID: PMC9324991.Narasimhan et al. (2021) Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking. Plant Physiol. 2021 Mar 18:kiab134. doi: 10.1093/plphys/kiab134. Epub ahead of print. PMID: 33734402.Hurny et al. (2020). SYNERGISTIC ON AUXIN AND CYTOKININ 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance. Nat Commun. 2020 May 1;11(1):2170. doi: 10.1038/s41467-020-15895-5. (immunolocalization)Kuang et al. (2019). Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins. Front Plant Sci. 2019 May 15;10:641. doi: 10.3389/fpls.2019.00641. eCollection 2019.Singh et al. (2018). A single class of ARF GTPase activated by several pathway-specific ARF-GEFs regulates essential membrane traffic in Arabidopsis. PLoS Genet. 2018 Nov 15;14(11):e1007795. doi: 10.1371/journal.pgen.1007795.
Special application note:
Cellular [compartment marker] of Golgi in immunolocalization and COP1 in western blot
Arginase-1, encoded by the ARG1 gene, is a cytosolic metalloenzyme expressed predominantly in hepatocytes. Arginase-1 plays a key role in the urea cycle by catalyzing the hydrolysis of arginine to ornithine and urea. Argininemia is an inherited autosomal recessive disorder characterized by a buildup of arginine and ammonia in the blood. Anti-Arginase-1 is highly specific for hepatocytes, and is therefore a sensitive and specific marker of benign and malignant hepatic tumours.
Arginase-1, encoded by the ARG1 gene, is a cytosolic metalloenzyme expressed predominantly in hepatocytes. Arginase-1 plays a key role in the urea cycle by catalyzing the hydrolysis of arginine to ornithine and urea. Argininemia is an inherited autosomal recessive disorder characterized by a buildup of arginine and ammonia in the blood. Anti-Arginase-1 is highly specific for hepatocytes, and is therefore a sensitive and specific marker of benign and malignant hepatic tumours.
Arginase-1, encoded by the ARG1 gene, is a cytosolic metalloenzyme expressed predominantly in hepatocytes. Arginase-1 plays a key role in the urea cycle by catalyzing the hydrolysis of arginine to ornithine and urea. Argininemia is an inherited autosomal recessive disorder characterized by a buildup of arginine and ammonia in the blood. Anti-Arginase-1 is highly specific for hepatocytes, and is therefore a sensitive and specific marker of benign and malignant hepatic tumours.
Actin-related proteins (ARPs) are found in the nuclei of all eukaryotic cells, but their functions are generally understood only in the context of their presence in various yeast and animal chromatin-modifying complexes. Arabidopsis thaliana ARP6 is a clear homolog of other eukaryotic ARP6s, including Saccharomyces cerevisiae ARP6, which was identified as a component of the SWR1 chromatin remodeling complex. Arabidopsis ARP6 is localized to the nucleus during interphase but dispersed away from the chromosomes during cell division. ARP6 expression was observed in all vegetative tissues as well as in a subset of reproductive tissues. Null mutations in ARP6 caused numerous defects, including altered development of the leaf, inflorescence, and flower as well as reduced female fertility and early flowering in both long- and short-day photoperiods.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -80 C; Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Arabidopsis thaliana
Immunogen:
ARP6 of Arabidopsis thaliana, UniProt: Q8LGE3, TAIR: At3g33520
Applications:
ELISA (ELISA), Immunoflourescence (IF), Western blot (WB)
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Deal et al. (2005). The nuclear actin-related protein ARP6 is a pleiotropic developmental regulator required for the maintenance of FLOWERING LOCUS C expression and repression of flowering in Arabidopsis. Plant Cell Oct;17(10):2633-46. doi:10.1105/tpc.105.035196.
Actin-related proteins (ARPs) are found in the nuclei of all eukaryotic cells, but their functions are generally understood only in the context of their presence in various yeast and animal chromatin-modifying complexes. Arabidopsis ARP8 shows 30 and 29% amino acid identity to yeast actin and Arabidopsis ACT2 in the regions of alignment, respectively. Because it is not closely related to yeast or human ARP8 and shows similar weak homology to yeast ARP8 and ARP9, the Arabidopsis ARP8 is considered a plant-specific orphan ARP.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -80 C; Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Antibody is recognizing following epitope, amino acids 447-471: SNLSIFPGPWCITRKQFRRKSRLMWThis antibody is recognizing the full-length 52 kDa recombinant ARP8 protein expressed in Escherichia coli as well as endogenous ARP8 of identical molecular weight in Arabidopsis thaliana extracts from different tissues: all vegetative and reproductive organs examined including seedlings, roots and siliques, with higher concentrations of the protein detected in developing flower buds and flowers within the inflorescence.
Application Details:
assay dependent
Conjugation:
IgG1
Isotype:
IgG1
Purity:
Cell culture supernatant
Molecular Weight:
52 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Kandasamy et al. (2008). ACTIN-RELATED PROTEIN8 encodes an F-box protein localized to the nucleolus in Arabidopsis. Plant Cell Physiol. 49(5):858-63. doi: 10.1093/pcp/pcn053.
Actin-related proteins (ARPs) are found in the nuclei of all eukaryotic cells, but their functions are generally understood only in the context of their presence in various yeast and animal chromatin-modifying complexes. Arabidopsis ARP8 shows 30 and 29% amino acid identity to yeast actin and Arabidopsis ACT2 in the regions of alignment, respectively. Because it is not closely related to yeast or human ARP8 and shows similar weak homology to yeast ARP8 and ARP9, the Arabidopsis ARP8 is considered a plant-specific orphan ARP.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -80 C; Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica rapa, Camelina sativa, Capsella rubella, Eutrema salsugineum, Raphanus sativusSpecies of your interest not listed? Contact us
Antibody is recognizing following epitope, amino acids 2-26: aa 2-ILKKVWG SVWNRSNSGKDLVNHQRA-26 This antibody is recognizing the full-length 52 kDa recombinant ARP8 protein expressed in Escherichia coli as well as endogenous ARP8 of identical molecular weight in Arabidopsis thaliana extracts from different tissues: all vegetative and reproductive organs examined including seedlings, roots and siliques, with higher concentrations of the protein detected in developing flower buds and flowers within the inflorescence.
Application Details:
assay dependent
Conjugation:
IgG1
Isotype:
IgG1
Purity:
Cell culture supernatant
Molecular Weight:
52 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Kandasamy et al. (2008). ACTIN-RELATED PROTEIN8 encodes an F-box protein localized to the nucleolus in Arabidopsis. Plant Cell Physiol. 49(5):858-63. doi: 10.1093/pcp/pcn053.
ARSA (targeting of tail-anchored proteins) is an arsenite transporter, localized in a cytoplasm and required for biogenesis of photosynthetic complexs.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Immunogen:
His-tagged, full length, recombinant ARSA of Chlamydomonas reinhardtii, overexpressed in E.coli, Cre24.g755097 (phytozome)
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Formigheri et al. (2013). Biogenesis of photosynthetic complexes in the chloroplast of Chlamydomonas reinhardtii requires ARSA1, a homolog of prokaryotic arsenite transporter and eukaryotic TRC40 for guided entry of tail-anchored proteins. Plant J. 2013 Mar;73(5):850-61. doi: 10.1111/tpj.12077. Epub 2012 Dec 28.
Histone-lysine N-methyltransferase ash1 is a Trithorax group (TrxG) protein that has histone methyltransferase activity. Specifically trimethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Drosophila melanogaster
Expected Species:
Drosophila melanogaster
Immunogen:
N-terminal GST-fusion of the peptide containing amino acids 1756-1855 of the Drosophila melanogaster Ash1protein, UniProt: Q9VW15
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Kahn et al. (2016). Interdependence of PRC1 and PRC2 for recruitment to Polycomb Response Elements. Nucleic Acids Res. 2016 Aug 23. pii: gkw701. [Epub ahead of print].Lee et al. (2015). Genome-wide activities of Polycomb complexes control pervasive transcription. Genome Res. 2015 Aug;25(8):1170-81. doi: 10.1101/gr.188920.114. Epub 2015 May 18.
ASN (Glutamine-dependent asparagine synthetase) is essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem. ASN2 is expressed in leaf and ASN1 is expressed in floral organs. The amino acid sequences of Arabidopsis thaliana ASN1 and ASN2 are 76% identical. The amino acid sequences of Arabidopsis thaliana and Zea mays ASN2 are 73.6% identical.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
Brassica rapa, Camelina sativa, Capsella rubella, Eutrema salsugineum, Punica granatumSpecies of your interest not listed? Contact us
Immunogen:
Purified full length, tag cleaved, recombinant Arabidopsis thaliana ASN2, UniProt: Q9LV77 , TAIR: AT5G65010
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Molecular Weight:
65 | 65 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Gaufichon et al. (2017). ASN1-encoded asparagine synthetase in floral organs contributes to nitrogen filling in Arabidopsis seeds. Plant J. 2017 Aug;91(3):371-393. doi: 10.1111/tpj.13567.Gaufichon et al. (2013). Arabidopsis thaliana ASN2 encoding asparagine synthetase is involved in the control of nitrogen assimilation and export during vegetative growth. Plant Cell Environ. 2013 Feb;36(2):328-42. doi: 10.1111/j.1365-3040.2012.02576.x.
Special application note:
This antibody reacts with both isoforms: ASN1 and ASN2
ASY1 (Asynapsis 1) is a protein required for normal meiosis in male and female gametophytes, which plays a crucial role in coordinating the activity of DMC1. Alternative names: Meiosis-specific protein ASY1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Hordeum vulgare
Expected Species:
Arabidopsis thaliana, Hordeum vulgare, Oryza sativa, Triticum aestivum, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
Recombinant ASY1 protein from Hordeum vulgare, UniProt: A0A8I6YI54
Pairing and synapsis of homologous chromosomes is required for normal chromosome segregation and the exchange of genetic material via recombination during meiosis. Synapsis is complete at pachytene following the formation of a tri-partite proteinaceous structure known as the synaptonemal complex (SC). In yeast, HOP1 is essential for formation of the SC, and localises along chromosome axes during prophase I. Homologues in Arabidopsis (AtASY1), Brassica (BoASY1) and rice (OsPAIR2) have been isolated through analysis of mutants that display decreased fertility due to severely reduced synapsis of homologous chromosomes. Analysis of these genes has indicated that they play a similar role to HOP1 in pairing and formation of the SC through localisation to axial/lateral elements of the SC.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Triticum aestivum
Expected Species:
Triticum aestivum
Immunogen:
KLH-conjugated synthetic pepitde chosen from Triticum aestivum ASY1 UniProt: A7TVU8
Total IgG. Protein G purified purified from Cell culture supernatant.
Reconstitution:
For reconstitution add 50 l of sterile water/tube
Molecular Weight:
66,3 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Lewandowska et al. (2021) The proteome of developing barley anthers during meiotic prophase I. J Exp Bot. 2021 Nov 10:erab494. doi: 10.1093/jxb/erab494. Epub ahead of print. PMID: 34758083.Darrier et al. (2019). Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High-Resolution Microscopy. Methods Mol Biol. 2020;2061:207-215. doi: 10.1007/978-1-4939-9818-0_15.
Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy. In analogy to many other amyloid associated disorders, alpha-synuclein may also form oligomeric assemblies. These small and soluble forms have been suggested to exert a stronger tissue damaging effect as compared to the monomeric and fibrillar counterpart. Using a recently developed technique a monoclonal oligomer-specific antibody has been designed.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
For short time storage add sodium azide and store at +4°C. For long time storage store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
synthetic peptide derived from human alpha-synuclein N-terminal Met1-Val15
Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
For short time storage add sodium azide and store at +4°C. For long time storage store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human
Expected Species:
Mouse
Immunogen:
synthetic peptide derived from human alpha-synuclein N-terminal Met1-Val15
Applications:
Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC)
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Tanudjojo et al. (2021) Phenotypic manifestation of ?-synuclein strains derived from Parkinson's disease and multiple system atrophy in human dopaminergic neurons. Nat Commun. 2021 Jun 21;12(1):3817. doi: 10.1038/s41467-021-23682-z. PMID: 34155194; PMCID: PMC8217249.
Special application note:
This antibody will recognize human SNCA monomers and multimers in Western blot. This antibody binds weakly to fibrills in IHC.Cross reactivity of this antibody to synuclein beta was not determined.This antibody can be used as a capture antibody in ELISA, combined with AS08 358 as a detection antibody.
Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy. In analogy to many other amyloid associated disorders, alpha-synuclein may also form oligomeric assemblies. These small and soluble forms have been suggested to exert a stronger tissue damaging effect as compared to the monomeric and fibrillar counterpart. Using a recently developed technique a monoclonal oligomer-specific antibody has been designed.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
For short time storage add sodium azide and store at +4°C. For long time storage store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Synthetic peptide derived from human alpha-synuclein Glu131-Ala140
Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy. In analogy to many other amyloid associated disorders, alpha-synuclein may also form oligomeric assemblies. These small and soluble forms have been suggested to exert a stronger tissue damaging effect as compared to the monomeric and fibrillar counterpart. Using a recently developed technique a monoclonal oligomer-specific antibody has been designed.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
For short time storage please add sodium azide and srote at +4°C.For long time storage store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human
Expected Species:
Mouse
Immunogen:
synthetic peptide derived from human alpha-synuclein Glu131-Ala140
Applications:
Dot blot (Dot), ELISA (ELISA), Immunolocalization (IL)
Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy. In analogy to many other amyloid associated disorders, alpha-synuclein may also form oligomeric assemblies. These small and soluble forms have been suggested to exert a stronger tissue damaging effect as compared to the monomeric and fibrillar counterpart. Using a recently developed technique a monoclonal oligomer-specific antibody has been designed.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Synthetic peptide derived from human alpha-synuclein Gly111-Tyr125
Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy. In analogy to many other amyloid associated disorders, alpha-synuclein may also form oligomeric assemblies. These small and soluble forms have been suggested to exert a stronger tissue damaging effect as compared to the monomeric and fibrillar counterpart. Using a recently developed technique a monoclonal oligomer-specific antibody has been designed.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
For short time storage add sodium azide and store at +4°C. For long time storage store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human, mouse
Immunogen:
Synthetic peptide derived from human alpha-synuclein Gly111-Tyr125
Applications:
Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC)
Limegrover et al. (2021) Sigma-2 receptor antagonists rescue neuronal dysfunction induced by Parkinson's patient brain-derived a-synuclein. J Neurosci Res. 2021 Apr;99(4):1161-1176. doi: 10.1002/jnr.24782. Epub 2021 Jan 22. PMID: 33480104.Kilpel inen et al. (2019). Behavioural and dopaminergic changes in double mutated human A30P*A53T alpha-synuclein transgenic mouse model of Parkinson s disease.Sci Rep. 2019 Nov 22;9(1):17382. doi: 10.1038/s41598-019-54034-z.Wu et al. (2017). The critical role of Nramp1 in degrading a-synuclein oligomers in microglia under iron overload condition. Neurobiol Dis. 2017 Aug;104:61-72. doi: 10.1016/j.nbd.2017.05.001. (human, mouse, immunolocalization)Svarcbahs et al. (2016). Inhibition of Prolyl Oligopeptidase Restores Spontaneous Motor Behavior in the a-Synuclein Virus Vector-Based Parkinson's Disease Mouse Model by Decreasing a-Synuclein Oligomeric Species in Mouse Brain. J Neurosci. 2016 Dec 7;36(49):12485-12497.Br nnstr m et al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.
Special application note:
This antibody is specific to oligomers in ELISA as a capture antibody. For specific details, please check: Br nnstr m et al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.
At2g21960 is an integral component of thylakoid membrane.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Higher plants Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide, derived from Arabidopsis thaliana, UniProt: Q9SJ03, TAIR: AT2G21960
Mouse anti-Ataxin-2 binding protein 1 (A2BP1) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Fox1 is one of a family of 3 mammalian Fox1 homologues. Fox1 was discovered in C. elegans as a gene involved in sex determination, and the name Fox is an acronym of "Feminizing locus on X" (1). The C. elegans Fox1 protein and its 3 mammalian homologues are all about 46 kDa proteins and each includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70 amino acid structure consisting of 4 beta strands and two alpha-helices. This region is identical in all three mammalian Fox1 family proteins. An alternate name for Fox 1 is ataxin-2 binding protein 1 (A2BP1), since it was discovered in a yeast two hybrid screen using ataxin-2 as bait (2). As with the other Fox proteins, it is assumed that Fox1/A2BP1 has a role in the RNA splicing in the nervous system (3). Fox3, a protein also known as NeuN, is a widely used marker of neuronal nuclei and proximal cytoplasm (4, 5). Like Fox3, Fox1 is expressed in neuronal nuclei, but with a different pattern of expression than Fox3. For example, in the cerebellum Fox3 does not stain Purkinje neurons and Golgi neurons, but these two neuron types are stained by Fox1 antibody (6).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
N-terminal 100 amino acids of human Fox1 as expressed in and purified from E. coli.
Applications:
IHC-Frozen,WB
Clone number:
1G10
Antibody Isotype:
IgG1
Application Details:
Immunocytochemistry (IHC) and Western Blotting (WB). A dilution of 1:1,000-1:2,000 is recommended for WB. A dilution of 1:500-1:1000 is recommended for ICC. The optimal dilution should be determined by the end user.
Alternative Names:
ataxin-2 binding protein 1 (A2BP1); RNA binding protein fox-1 homolog 1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody reacts with a 48 kDa and 46 kDa band by Western blot on extract from mouse brain. It has also been used successfully for immunocytochemistry on rat neuronal cultures.
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
ATG12b (Autophagy-related protein 12b) is an Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt) and autophagy vesicles formation. Alternative names: Ubiquitin-like protein ATG12B, APG12-like protein b, AtAPG12b.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C (short tem, months) or at -80°C(long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
Recombinant, full length ATG12b ofArabidopsis thaliana protein sequence UniProt: Q9LVK3-1, TAIR: At3g13970
This antibody may also recognise ATG12a; in wild-type, ATG12 is also conjugated to ATG5
Application Details:
1 : 3000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l, of sterile water
Molecular Weight:
10,4 | 12 and 50 kDa in wilde type plants and 12 kDa in some autophagy deficient mutants
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Chung et al. (2010). ATG8 lipidation and ATG8-mediated autophagy in Arabidopsis require ATG12 expressed from the differentially controlled ATG12A AND ATG12B loci. Plant J. 2010 May;62(3):483-93. doi: 10.1111/j.1365-313X.2010.04166.x.
ATG13a (Autophagy-related protein 13a) is involved in autophagy in a nutritional condition dependent manner. The ATG1-ATG13 protein kinase complex regulates downstream events required for autophagosome enclosure and/or vacuolar delivery. Alternative names: AtAPG13a.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C (short tem, months) or at -80°C(long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Noccaea caerulescensSpecies of your interest not listed? Contact us
Immunogen:
Full length, recombinant ATG13a of Arabidopsis thaliana protein sequence UniProt: F4IXZ6, TAIR: At3g49590 with N-terminal HIS-tag.
Antibody may also recognise ATG13b; multiple bands observed due to phosphorylation
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l, of sterile water
Molecular Weight:
68,4 | 70-80 kDa
Not reactive in:
Oryza sativa
Selected references:
Suttangkakul et al. (2011). The ATG1/ATG13 protein kinase complex is both a regulator and a target of autophagic recycling in Arabidopsis. Plant Cell. 2011 Oct;23(10):3761-79. doi: 10.1105/tpc.111.090993.
ATG16 (Autophagy-related protein 16) may play a role in autophagy process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C (short tem, months) or at -80°C(long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
N-terminal part of of ATG16 of Arabidopsis thaliana UniProt: Q6NNP0, TAIR: At5g50230
ATG1a (Serine/threonine-protein kinase ATG1a) is a serine/threonine protein kinase involved in autophagy in a nutritional condition-dependent manner. The ATG1-ATG13 protein kinase complex regulates downstream events required for autophagosome enclosure and/or vacuolar delivery. Becomes a target of autophagy under nutrient starvation and connects autophagy to plant nutritional status. Alternative names: Autophagy-related protein 1a, AtAPG1a.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C (short tem, months) or at -80°C(long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Noccaea caerulescensSpecies of your interest not listed? Contact us
Immunogen:
Full length, N-terminally HIS-tagged ATG1a of Arabidopsis thaliana protein sequence UniProt: Q94C95, TAIR: At3g61960, overexpressed in E.coli and injected as a gel piece
Antibody may also recognise ATG1b and ATG1c; does not recognise ATG1t, Suttangkakul et al. (2011).
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l, of sterile water
Molecular Weight:
69,6 | 70 kDa
Not reactive in:
Oryza sativa
Selected references:
Suttangkakul et al. (2011). The ATG1/ATG13 protein kinase complex is both a regulator and a target of autophagic recycling in Arabidopsis. Plant Cell. 2011 Oct;23(10):3761-79. doi: 10.1105/tpc.111.090993.
ATG3 (Autophagy-related protein 3) is a E2 conjugating enzyme responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8. This step is required for the membrane association of ATG8. Alternative names: Autophagy-related E2-like conjugation enzyme ATG3, AtAPG3, Protein autophagy 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C (short tem, months) or at -80°C(long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica campestris, Eutrema salsugineum, Helianthus annuus, Nicotiana tabacum, Noccaea caerulescens, Populus trichocarpa, Salvia splendens, Sisymbrium irio, Solanum tuberosum Species of your interest not listed? Contact us
Immunogen:
Full length recombinant ATG3 of Arabidopsis thaliana protein sequence UniProt: Q0WWQ1-1, TAIR: At5g61500, overexpressed in E.coli
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Phillips et al. (2008). The ATG12-conjugating enzyme ATG10 Is essential for autophagic vesicle formation in Arabidopsis thaliana. Genetics. 2008 Mar;178(3):1339-53. doi: 10.1534/genetics.107.086199.
ATG4 (Autophagy protein 4) is a protein with cyteine-type endopeptidase activity involved in autophagy process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Technical details how to work with this antibody are provided here: P rez-P rez et al. (2016). Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease. Plant Physiol. 2016 Dec;172(4):2219-2234.
Chen et al. (2016). The role of nitric oxide signalling in response to salt stress in Chlamydomonas reinhardtii. Planta. 2016 Sep;244(3):651-69. doi: 10.1007/s00425-016-2528-0. Epub 2016 Apr 26.P rez-P rez et al. (2016). Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease. Plant Physiol. 2016 Dec;172(4):2219-2234.
Special application note:
This antibody is recognizing 25 ng of recombinant CrATG4This product can be sold containing ProClin if requested.
ATG5 (Autophagy protein 5) is a protein required for autophagy. Involved in a negative feedback loop that modulates NPR1-dependent salicylic acid (SA) signaling and limits senescence and immunity-related programmed cell death (PCD) in plants and a complete proteolysis of chloroplast stroma proteins in senescent leaves, in the degradation of damaged peroxisomes. Alternative names: Protein autophagy 5, AtAPG5.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C (short tem, months) or at -80°C(long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Solanum lycopersicum, Solanum tuberosum
Immunogen:
Full length, recombinant ATG5 of Arabidopsis thaliana protein sequence UniProt: Q9FFI2-1, TAIR: At5g17290, overexpressed in E.coli
In wild-type plants almost all ATG5 is conjugated to ATG12.This item can be sold containing ProClin
Application Details:
1 : 3000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l, of sterile water
Molecular Weight:
38,5 | 50 kDa (in wilde type plants) and ca, 38 kDa in autophagy defective mutants
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Thompson et al. (2005). Autophagic nutrient recycling in Arabidopsis directed by the ATG8 and ATG12 conjugation pathways. Plant Physiol. 2005 Aug;138(4):2097-110.
ATG5 (Autophagy-related protein 5) the protein forms a conjugate with ATG12 with an essential role in plant nutrient recycling. It is involved in complete proteolysis of chloroplast stroma proteins in senescent leaves and degradation of damaged peroxisomes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Recombinant ATG5 of Arabidopsis thaliana
Immunogen:
Recombinant ATG5 of Arabidopsis thaliana, UniProt:Q9FFI2, TAIR:AT5G17290
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Young et al. (2019). A facile forward-genetic screen for Arabidopsis autophagy mutants reveals twenty-one loss-of-function mutations disrupting six ATG genes. Autophagy. 2019 Jun;15(6):941-959. doi: 10.1080/15548627.2019.1569915.
Special application note:
This antibody is so far recognizing recombinant ATG5 from Arabidopsis thaliana and reactivity on endogenous protein needs to be confrimed. It does not react with 6xHis-ATG7.This product can be sold containing ProClin if requested.
ATG7 (Autophagy-related protein 7) is an E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Acts in the senescence process, degradation of damaged peroxisomes and non-selective degradation of chlorophylls and photosynthetic proteins during stress-induced leaf yellowing.Alternative names: Ubiquitin-like modifier-activating enzyme atg7, ATG12-activating enzyme E1 atg7, AtAPG7, Protein PEROXISOME UNUSUAL POSITIONING 4.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C (short tem, months) or at -80°C(long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica rapa, Capsella rubella , Citrus clementina, Noccaea caerulescens Species of your interest not listed? Contact us
Immunogen:
Recombinant ATG7 of Arabidopsis thaliana, UniProt: Q94CD5-1, TAIR: At5g45900, overexpressed in E.coli, collected in inclusion bodies, which were solubilized before immunization
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Doelling et al. (2002). The APG8/12-activating enzyme APG7 is required for proper nutrient recycling and senescence in Arabidopsis thaliana. J Biol Chem. 2002 Sep 6;277(36):33105-14.
Special application note:
Protein name was changed from APG7 to ATG7 after Doelling paper was published.
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Actinidia chinensis var. chinensis, Cajanus cajan, Capsicum annuum, Corchorus olitorius, Cucumis melo, Glycine max, Glycine soja, Gossypium arboreum, Juglans regia, Malus domestica, Nelumbo nucifera, Nicotiana tabacum, Nicotiana benthamiana, Nicotiana sylvestris, Noccaea caerulescens, Prunus yedoensis var. nudiflora, Solanum chacoense, Solanum lycopersicum, Vigna radiataSpecies of your interest not listed? Contact us
Immunogen:
Recombinant Arabidopsis thaliana ATG7 UniProt Q94CD5, TAIR AT5G45900
ATG8 (Autophagy-related protein) involved in degradation and recycling of intracellular components in a process of autophagy.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Recombinant ATG8 A-I of Arabidopsis thaliana
Immunogen:
Part of recombinant ATG8A from Arabidopsis thaliana, UniProt:Q8LEM4,TAIR:AT4G21980
ATG8 (Autophagy-related protein 8) is involved in degradation and recycling of intracellular components in a process of autophagy. ATG8 is a molecular autophagy marker in Chlamydomonas reinhardtii (P rez-P rez et al. 2010, Plant Physiol. 152: 1874-88).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For Arabidopsis thaliana the signal obtained using ATG8 antibodies is cleaner in case of roots compare to leaf material. For best results please follow extraction protocol described in lvarez et al. (2012). ATG8 signal corresponds to the two bands of 17 kDa.Preparation of a cell extract from Arabidopsis thaliana:A. Plants were first subjected to autophagy activating conditions: nutrient (nitrogen or carbon) limitation or oxidative stress in order to activate this degradative process.B. Total protein extracts can be obtained as described by lvarez. Leaves are grinded in liquid nitrogen with a minimal volume of extraction buffer (100 mM Tris-HCl pH 8, 400 mM sucrose, 1 mM EDTA, 0.1 mM phenylmethylsulfonyl fluoride (PMSF), 10 mg/ml sodium deoxycholate, 10 g/ml of leupeptin, 10 g/ml of pepstatin A, 4% (v/v) protease inhibitor cocktail from Roche).C. Cell debris is removed by centrifuging at 500 g for 10 min at 4 C.Important note:It is recommendable to use bigger gels in order to get a better resolution of ATG8 bands. Midi-protean gels are better than mini-gels. There are 9 ATG8 isoforms and this antibody will likely recognizes all of them.For immunolocalization protocol, please inquire.
Application Details:
1 : 1000 (IL), 1 : 1000-1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
15,2 | 15 kDa
Not reactive in:
Cuscuta chinensis
Selected references:
Sun et al. (2022) Genome of Paspalum vaginatum and the role of trehalose mediated autophagy in increasing maize biomass. Nat Commun. 2022;13(1):7731. Published 2022 Dec 13. doi:10.1038/s41467-022-35507-8Cao et al. (2022) Autophagic pathway contributes to low-nitrogen tolerance by optimizing nitrogen uptake and utilization in tomato. Hortic Res. 2022 Mar 23;9:uhac068. doi: 10.1093/hr/uhac068. PMID: 35669705; PMCID: PMC9164271.Samperna et al (2022). Cyclopaldic Acid, the Main Phytotoxic Metabolite of Diplodia cupressi, Induces Programmed Cell Death and Autophagy in Arabidopsis thaliana. Toxins (Basel). 2022 Jul 11;14(7):474. doi: 10.3390/toxins14070474. PMID: 35878212; PMCID: PMC9325063.Zharova et al. (2022) Role of Autophagy in Haematococcus lacustris Cell Growth under Salinity. Plants. 2022; 11(2):197. https://doi.org/10.3390/plants11020197 (immunofluorescence)Mishra et al. (2021) Interplay between abiotic (drought) and biotic (virus) stresses in tomato plants. Mol Plant Pathol. 2021 Dec 30. doi: 10.1111/mpp.13172. Epub ahead of print. PMID: 34970822.
Special application note:
This product can be sold containing ProClin if requested.This antibody is recognizing 1 ng of recombinant CrATG8. Antigen used to elicit this antibody is conserved from 70-80 % in following ATG protein from Arabidopsis thaliana: ATG8a UniProt: Q8LEM4 ATG8B UniProt: Q9XEB5 ATG8c UniProt: Q8S927 ATG8d UniProt: Q9SL04, ATG8e UniProt: Q8S926 ATG8f UniProt: Q8VYK7 and conserved below 70 % in: ATG8g UniProt: Q9LZZ9 ATG8h Uniprot: Q8S92This antibody does not recognize all isoforms into the same degree.
ATG9 (Autophagy-related protein 9) is required for autophagy that plays an essential role in plant nutrient recycling. Alternative name: AtAPG9.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Plant ATG9 is most likely N-glycosylated, and glycosylated membrane proteins can migrate as more than one form in SDS-PAGE (smear); the protein most likely forms functional dimers which could be detected also after western blotting. Usage of microsomal fraction is highly recommended.
ATG9 (Autophagy-related protein 9) is required for autophagy that plays an essential role in plant nutrient recycling. Alternative name: AtAPG9.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica campestris, Brassica rapa subsp. pekinensis, Capsella rubella, Eutrema salsugineum Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana ATG9 protein sequence, N-terminal part, UniProt: Q8RUS5, TAIR: AT2G31260
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Colemanosphaera charkowiensis, Eudorina elegans,Gonium pectorale, Pandorina colemaniae, Pleodorina starrii, Volvox africanus, Yamagishiella unicocca Species of your interest not listed? Contact us
Immunogen:
CF 1 alpha subunit of the chloroplast ATP synthase complex isolated from Chlamydomonas reinhardtii, UniProt: P26526
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Dicots and (including Lilium superbum) chloroplast AtpA; may cross-react with mitochondrial AtpA; Algae, Nannochloropsis gaditana, CyanobacteriaSpecies of your interest not listed? Contact us
Immunogen:
Recombinant maize chloroplast AtpA P05022
Applications:
Immunogold (IG), Immunoprecipitation (IP), Western blot (WB)
Pao et al. (2018). Lamelloplasts and minichloroplasts in Begoniaceae: iridescence and photosynthetic functioning. J Plant Res. 2018 Mar 2. doi: 10.1007/s10265-018-1020-2. (ImmunoGold)Zhang et al. (2017). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989.Jeon et al. (2017). Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Plant Mol Biol. 2017 Nov;95(4-5):463-479. doi: 10.1007/s11103-017-0664-y.Murcha et al. (2016). Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016.Camejo et al. (2015). Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits. Proteomics. 2015 Aug;15(15):2634-42. doi: 10.1002/pmic.201400370.Yang et al. (2015). Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 May 12. pii: S1046-5928(15)00111-4. doi: 10.1016/j.pep.2015.05.006.
Special application note:
Sequence of the protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757
AAA2 domain is present in ATP binding proteins involved in stress response and being members of clpA/clpB family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 23,3 g/ l
Storage Temp:
Store at 2-8°C.; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3
Applications:
Immunolocalization (IL), Immunogold (IG), Western blot (WB)
Neusius et al. (2022) Lysine acetylation regulates moonlighting activity of the E2 subunit of the chloroplast pyruvate dehydrogenase complex in Chlamydomonas. Plant J. 2022 Sep;111(6):1780-1800. doi: 10.1111/tpj.15924. Epub 2022 Aug 8. PMID: 35899410.Levitan et al. (2019). Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17316-17322. doi: 10.1073/pnas.1906726116.Nelson et al. (2019). Protein lysine methylation contributes to modulating the response of sensitive and tolerant Arabidopsis species to cadmium stress. doi: 10.1111/pce.13692.Gellert et al. (2018). A single point mutation on the cucumber mosaic virus surface induces an unexpected and strong interaction with the F1 complex of the ATP synthase in Nicotiana clevelandii plants. Virus Res. 2018 Jun 2;251:47-55. doi: 10.1016/j.virusres.2018.05.005. (immunogold)Quesada et al. (2011). Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development. Plant J. Nov;68(4):738-53. doi: 10.1111/j.1365-313X.2011.04726.x. Epub 2011 Sep 13.
Special application note:
The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 ATP synthase and most known bacterial F-type ATP synthases, Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Acinetobacter baumannii, Algae, Cannabis sativa, Clostridium sp., Diatoms, Cyanobacteria, E.coli K-12, Vitis vinifera, Yersinia sp. Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1 At5g08670 as well as Chlamydomonas reinhardtii P06541 and A8IQU3
Applications:
Blue Native-PAGE (BN-PAGE), Immunofluorescence (IF), Western blot (WB)
Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 g/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria, Bacillus cereus.
Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.Aihara et al. (2019). Algal photoprotection is regulated by the E3 ligase CUL4-DDB1DET1. Nat Plants. 2019 Jan;5(1):34-40. doi: 10.1038/s41477-018-0332-5.Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)Rurek et al. (2015). Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. Biochim Biophys Acta. 2015 Jan 21. pii: S0005-2728(15)00016-X. doi: 10.1016/j.bbabio.2015.01.005.Eom et al. (2014). Bacillus subtilis HJ18-4 from Traditional Fermented Soybean Food Inhibits Bacillus cereus Growth and Toxin-Related Genes. J Food Sci. 2014 Nov;79(11):M2279-87. doi: 10.1111/1750-3841.12569. Epub 2014 Oct 30.
Special application note:
This product can be sold containing proClin if requested.The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 Atp Synthase, and most known bacterial F-type Atp Synthases. Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein.
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3
Applications:
Blue Native-PAGE (BN-PAGE), Immunofluorescence (IF), Ultrastructure Expansion Microscopy (U-ExM), Western blot (WB)
Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 g/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria, Bacillus cereus.
Lim et al (2022). Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13(1):652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022). https://doi.org/10.1038/s41586-022-04662-9Bychkov et al. (2022) The role of PAP4/FSD3 and PAP9/FSD2 in heat stress responses of chloroplast genes. Plant Sci. 2022 Sep;322:111359. doi: 10.1016/j.plantsci.2022.111359. Epub 2022 Jun 20. PMID: 35738478.Ripamonti et al (2022). Silencing of ATP Synthase Beta Impairs Egg Development in the Leafhopper Scaphoideus titanus, Vector of the Phytoplasma Associated with Grapevine Flavescence Doree. International Journal of Molecular Sciences. 2022; 23(2):765. https://doi.org/10.3390/ijms23020765Tanno et al. (2021) The four-celled Volvocales green alga Tetrabaena socialis exhibits weak photobehavior and high-photoprotection ability. PLoS One. 2021 Oct 26;16(10):e0259138. doi: 10.1371/journal.pone.0259138. PMID: 34699573; PMCID: PMC8547699.
Special application note:
The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 Atp Synthase, and most known bacterial F-type Atp Synthases. Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein.Anti-AtpB antibody was used as a loading control in Chlamydomonas reinhardtii and Synechocystis sp. PCC6803.This product can be sold containing proclin if requested
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. This enzyme is localised in mitochondrial inner membrane.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica oleracea var. botrytis cv. 'Diadom'
Expected Species:
Chlamydomonas reinhardtii, Nicotiana tabacum, Oryza sativa, Phaeodactylum tricornutum Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from available plant, algal mitochondrial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana ATP synthase subunit beta-1 UniProt:P83483, TAIR:AT5G08670ATP synthases subunit beta-2 UniProt:P83484, TAIR: AT5G08690, ATPase subunit beta-3, UniProt: Q9C5A9, TAIR:AT5G08680, which belong to mitochondrial respiratory chain complex I.
Wei et al. (2019). Arabidopsis mtHSC70-1 plays important roles in the establishment of COX-dependent respiration and redox homeostasis. J Exp Bot. 2019 Aug 6. pii: erz357. doi: 10.1093/jxb/erz357.
Special application note:
Lack of antibody reactivity was confirmed on chloroplast fraction.This product can be sold containing ProClin if requested.
ATP synthase is the universal enzyme that stnthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.This product is a recombinant protein standard, source: Synechocystis strain PCC 6803.
Product Type:
Antibody
Format:
Lyophilized, in glycerol.
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Concentration: after adding 90 l of dest. water final concentration of the standard is 0.27 pmol/ l.Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:
Standard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2μg of chlorophyll will give a AtpB signal in this range.Positive control: load per well: a 2μl load is optimal for most chemiluminescent detection systems.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:
For reconstitution add 90 l of milliQ water, Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized
Molecular Weight:
in most gel systems AtpB migrates around 50-54 kDa
Selected references:
Fraser et al. (2013). Photophysiological and Photosynthetic Complex Changes during Iron Starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942. PLOS ONE.
Special application note:
The AtpB protein standard can be used in combination with global anti-AtpB antibodies to quantitate AtpB from a wide range of species. Global antibodies are raised against highly conserved amino acid sequences in the AtpB protein.Quantitative western blot: detailed method description, video tutorial
ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex. Alternative name of gamma subunit is also: F-ATPase gamma subunit.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Apparent molecular weight of subunit gamma (and as general rule most of ATP synthase subunits) is quite different between Chlamydomonas (42 kDa) and higher plants (38 kDa in spinach), see figure in Lemaire et al. (1989).
Pipitone et al. (2021). A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis. Elife. 2021 Feb 25;10:e62709. doi: 10.7554/eLife.62709. PMID: 33629953; PMCID: PMC7906606.Storti et al. (2020). The activity of chloroplast NADH dehydrogenase-like complex influences the photosynthetic activity of the moss Physcomitrella patens. doi.org/10.1101/2020.01.29.924597Pralon et al. (2019). Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019 Jun 20;2:220. doi: 10.1038/s42003-019-0477-4.Li et al. (2019). A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nat Genet. 2019 Apr;51(4):627-635. doi: 10.1038/s41588-019-0370-6.Liang et al. (2018). Thylakoid-Bound Polysomes and a Dynamin-Related Protein, FZL, Mediate Critical Stages of the Linear Chloroplast Biogenesis Program in Greening Arabidopsis Cotyledons. Plant Cell. 2018 Jul;30(7):1476-1495. doi: 10.1105/tpc.17.00972. Epub 2018 Jun 7.
Special application note:
This product can be sold containing ProClin if requested
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at at Store short-term 4 C, Long-term -20 . Repeated freezing and thawing is not recommended.
This product can be sold with ProClin if requested
Application Details:
1 : 2000 (WB)
Purity:
Serum
Molecular Weight:
23 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Blair et al. (2018). The Helicobacter pylori cell shape promoting protein Csd5 interacts with the cell wall, MurF, and the bacterial cytoskeleton. Mol Microbiol. 2018 Jul 24. doi: 10.1111/mmi.14087.Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4 C, Long-term -20 . Repeated freezing and thawing is not recommended. It ontains 0,01% sodium azide.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Ostreococcus lucimarinusm, Spinacia oleracea, Sorghum bicolor, Volvox carteri Species of your interest not listed? Contact us
Immunogen:
isolated CF1 subunit of the chloroplast ATP synthase complex from Chlamydomonas reinhardtii Q42687.1
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Perlaza (2021). Organelle Size and Quality Control in Chlamydomonas Reinhardtii. UCSF. ProQuest ID: Perlaza_ucsf_0034D_12217. Merritt ID: ark:/13030/m5257z1d. Retrieved from https://escholarship.org/uc/item/1jg3874hPerlaza et al. (2019). The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response. Elife. 2019 Oct 15;8. pii: e49577. doi: 10.7554/eLife.49577. (immunofluorescence)
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4 C, Long-term -20 . Repeated freezing and thawing is not recommended. It ontains 0,01% sodium azide.
Host Animal:
Rabbit
Species Reactivity:
dicots, Chlamydomonas reinhardtii, cyanobacteria including Synechocystis sp. PCC 6803.
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
isolated CF1 subunit of the chloroplast ATP synthase complex from Chlamydomonas reinhardtii
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4 C, Long-term -20 . Repeated freezing and thawing is not recommended. It ontains 0,01% sodium azide.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Galvis et al. (2020). H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in chloroplast ATP synthase mutants. Plant Physiol. pp.01561.2019. doi: 10.1104/pp.19.01561.Koochak et al. (2019). The structural and functional domains of plant thylakoid membranes. Plant J. 2019 Feb;97(3):412-429. doi: 10.1111/tpj.14127.Lv et al. (2019). Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. Plant Cell. 2019 Jan;31(1):210-230. doi: 10.1105/tpc.18.00813.Gao et al. (2018). A supercomplex, approximately 720 kDa and composed of both photosystem reaction centers, dissipates excess energy by PSI in green macroalgae under salt stress. Plant Cell Physiol. 2018 Oct 8. doi: 10.1093/pcp/pcy201.Koochak et al. (2018). The structural and functional domains of plant thylakoid membranes. Plant J. 2018 Oct 12. doi: 10.1111/tpj.14127. (BN-PAGE)Rantala and Tikkanen et al. (2018). Phosphorylation‐induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2.Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658. Grieco et al. (2015). Light-harvesting II antenna trimers connect energetically the entire photosynthetic machinery - including both photosystems II and I. Biochim Biophys Acta. 2015 Jun-Jul;1847(6-7):607-19. doi: 10.1016/j.bbabio.2015.03.004. Epub 2015 Apr 3.Yap at al. (2015). AEF1/MPR25 is implicated in RNA editing of plastid atpF and mitochondrial nad5 and also promotes atpF splicing in Arabidopsis and rice. Plant J. 2015 Jan 13. doi: 10.1111/tpj.12756.
Special application note:
This product can be sold containing proClin if requested
ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.Alternative names: ATPase subunit II, ATP synthase F(0) sector subunit b'
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Chlamydomonas reinhardtii
Expected Species:
Algae, Oryza sativa, Sorghum bicolor, Zea mays, Volvox carteri Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated mix of synthetic peptide derived from Arabidopsis thaliana AtpG Q0WMW8, At4g32260 and Chlamydomonas reinhardtii ATP synthase subunit b' A8J785
F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Algae, Cannabis sativa, Glycine max, Oryza sativa, Physcomitrium patens, Pisum sativum, Populus alba, Pinus thunbergii, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
Purified c subunit from Spinacia oleracea UniProt: P69447.
Note that increased incubation at 95 C (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e,g, 5 or 6 subunits) may occur
Application Details:
1 : 1000-1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
8 kDa (for Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Nath et al. (2016). A Nitrogen-Fixing Subunit Essential for Accumulating 4Fe-4S-Containing Photosystem I Core Proteins. Plant Physiol. 2016 Dec;172(4):2459-2470. Epub 2016 Oct 26.Lawrence et al. (2010). Recombinant production and purification of the subunit c of chloroplast ATP synthase. Protein Expression and Purification 76: 15-24.
Special application note:
This product can be sold containing proclin if requested.
F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Chlamydomonas reinhardtii
Expected Species:
Algae, Cannabis sativa, Glycine max, Hordeum vulgare, Oryza sativa, Ostreococcus tauri, Physcomitrium patens, Pinus thunbergii, Pisum sativum, Populus alba, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptides derived from AtpH subunit c of Arabidopsis thaliana UniProt: P56760, TAIR: AtCg00140 and Chlamydomonas reinhardtii UniProt: Q37304
Please note that increased incubation at 95 C (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e,g, 5 or 6 subunits) may occur
Application Details:
1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
8 kDa (for Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Schulz et al. (2017). Molecular architecture of the N-type ATPase rotor ring from Burkholderia pseudomallei. EMBO Rep. 2017 Apr;18(4):526-535. doi: 10.15252/embr.201643374.
Special application note:
This product can be sold containing ProClin if requested
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient. The transmembrane CF0IV subunit of appr. 25 kDa belongs to a stator part of ATP synthase and is involved in the proton translocation.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4 C, Long-term -20 . Repeated freezing and thawing is not recommended. It ontains 0,01% sodium azide.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.
Atrazine is a herbicide against pre- and post-emergence broadleaf and grassy weeds. It is a very persistent compound and it has been banned in the EU since 2004. Its main toxic effect is as endocrine diruptor.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°Cup to one month or in aliquots at -20 °C for long time storage. Avoid repeated freezing and thawing.
Host Animal:
Sheep
Immunogen:
BSA-conjugated atrazine, Target: Atrazine, CAS no.: 1912-24-9 purchased from SIGMA
The specificity of the antibody was determined by measuring the cross-reactivity with a range of compounds in ELISA.Cross Reactivity%Atrazine100%Propazine150%Simazine4%Ametryn2500%Prometryn1700%Simetryn280%Terbutylazine2%Atrazine-desethyl7%Atrazine desisopropyl1%Atrazine-2-hydroxy1%Cinosulfuron<0.1%Prosulferon<0.1%Triasulfuron<0.1%Chlorpyrifos<0.1%Clofibrinezuur<0.1%Deltametrin<0.1%Diclofenac1%Etrimfos1%Erythromycine<0.1%Fenitrothion<0.1%Fenofibraat<0.1%Atrazine21%Ibuprofen<0.1%Malathion<0.1%Maleaminezuur<0.1%Metoprolol<0.1%Metacrifos<0.1%4-n-Nonylfenol2%Permetrin<0.1%Pirimifos-ethyl<0.1%Sulfadimidine<0.1%Vinclozolin<0.1%
The hormone Atrial natriuretic peptide (ANP) is produced by cardiomyocites and is secreted into the bloodstream. ANP acts on convoluted tubules in kidney reducing sodium absorption which results in reducing the blood pressure.
Synthetic rat Atrial natriuretic peptide (123-150 aa) conjugated to BSA.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human; mouse; rat. Highly conserved and expected to interact with feline, canine, porcine, bovine, and equine ANP.
Storage:
At least 12 months after purchase at 2 - 8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for up to 6 months for a higher stability and at 2-8°C with an appropriate antibacterial agent for up to 2 weeks.Avoid freeze-thaw cycles
ATRX is involved in the remodeling of the nucelosome structure, and facilitate the transcription and replication. ATRX loss occurs in grades II/III astrocytomas and glioblastomas and comes together with IDH1 mutations. Detection of ATRX with IHC acts a sensitive method to identify the mutations.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC703
Antibody Isotype:
IgG2b
GMDN Code:
65252
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
ATRX is involved in the remodeling of the nucelosome structure, and facilitate the transcription and replication. ATRX loss occurs in grades II/III astrocytomas and glioblastomas and comes together with IDH1 mutations. Detection of ATRX with IHC acts a sensitive method to identify the mutations.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC703
Antibody Isotype:
IgG2b
GMDN Code:
65252
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
ATRX is involved in the remodeling of the nucelosome structure, and facilitate the transcription and replication. ATRX loss occurs in grades II/III astrocytomas and glioblastomas and comes together with IDH1 mutations. Detection of ATRX with IHC acts a sensitive method to identify the mutations.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC703
Antibody Isotype:
IgG2b
GMDN Code:
65252
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
AURKAIP1 mouse (Aurora kinase A-interacting protein) may act as a negative regulator of Aurora-A kinase, by down-regulation through proteasome-dependent degradation. Alternative names: 28S ribosomal protein S38, mitochondrial (MRP-S38), AURKA-interacting protein.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Mouse
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Recombinat mouse AURKAIP1 protein expressed in E.coli, UniProt: Q9DCJ7
Rabbit anti-Autophagy protein 5 (ATG5) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. FUNCTION: May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. SUBCELLULAR LOCATION: Cytoplasm. Colocalizes with nonmuscle actin. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. INDUCTION: By apoptotic stimuli. PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane. SIMILARITY: Belongs to the ATG5 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to the C-terminal of human ATG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B has been used to orchestrate/boost the immune response.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy protein 5; APG5-like; APG 5; Apoptosis-specific protein; APG5; ATG5; APG5L; ASP
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
E.N. Wilson et al (2011) A Switch Between Cytoprotective and Cytotoxic Autophagy in the Radiosensitization of Breast Tumor Cells by Chloroquine and Vitamin D. Horm Cancer. 2011 Sep 2. Bristol ML et al (2012) Dual functions of autophagy in the response of breast tumor cells to radiation: cytoprotective autophagy with radiation alone and cytotoxic autophagy in radiosensitization by vitamin D 3. Autophagy. 2012 May 1;8(5):739-53. Guido C et al (2012) Metabolic reprogramming of cancer-associated fibroblasts by TGF-? drives tumor growth: connecting TGF-_ signaling with "Warburg-like" cancer metabolism and L-lactate production. Cell Cycle. 2012 Aug 15;11(16):3019-35.
Specificity:
IHC and WB confirmed the specificity for ATG5. Human, not yet tested in other species.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Autophagy protein 5 (ATG5) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. FUNCTION: May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. SUBCELLULAR LOCATION: Cytoplasm. Colocalizes with nonmuscle actin. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. INDUCTION: By apoptotic stimuli. PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane. SIMILARITY: Belongs to the ATG5 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to the C-terminal of human ATG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
Flow Cytometry (2 ?g/10^6 cells), IHC, immunofluorescence, WB. Use a dilution of 1:200 to 1:1000 for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy protein 5; APG5-like; APG 5; APG5; Apoptosis-specific protein; ATG5; APG5L; ASP
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Villanueva-Paz M. et al. (2016) Amitriptyline induces mitophagy that precedes apoptosis in human HepG2 cells. Genes Cancer. 2016;7(7-8):260-277 Garrido-Maraver J. et al (2012) Screening of effective pharmacological treatments for MELAS syndrome using yeasts, fibroblasts and cybrids models of the disease Br J Pharmacol. 2012 Jul 2. De la Mata M. et al (2012) Recovery of MERRF fibroblasts and cybrids pathophysiology by Coenzyme Q10 Neurotherapeutics. 2012 Apr;9(2):446-63. Cotan D. et al. (2011) Secondary coenzyme Q10 deficiency triggers mitochondria degradation by mitophagy in MELAS fibroblasts FASEB J. 2011
Specificity:
IHC and wb confirmed the specificity for ATG5. Human, not yet tested in other species.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Autophagy-related protein 10 (ATG10) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
ATG10 plays a role in autophagy. It is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugations and modificatioon of a soluble form of MAPLC3 1A, a homolog of yeast ATG8, to a membrane bound form. It is also able to directly interact either with ATG5 or ATG7.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A peptide (CLKNSQKINKNV) corresponding to the C-terminal of human ATG10 protein has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence. A dilution of 1:100 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy-related protein 10; APG10-like; ATG10
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Ryningen A et al (2012) Inhibition of Mammalian target of rapamycin in human acute myeloid leukemia cells has diverse effects that depend on the environmental in vitro stress. Bone Marrow Res. 2012;2012:329061.
Specificity:
IHC, WB and ELISA confirmed the specificity for ATG10. Human. Other species not yet tested.
Storage:
After reconstitution keep aliquots at minus 20ºC for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Autophagy-related protein 12 (ATG12) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Required for autophagy. SUBUNIT: Conjugated to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: Belongs to the ATG12 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to the N-terminal of human ATG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence, WB. A dilution of 1:200 to 1:1000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy-related protein 12; APG12-like; APG 12; ATG12; APG12; APG12L
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
IHC and WB confirmed the specificity for ATG12. Human, rat
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Autophagy-related protein 12 (ATG12) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Required for autophagy. SUBUNIT: Conjugated to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Ubiquitous. SIMILARITY: Belongs to the ATG12 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to the N-terminal of human ATG12 protein has been used as the immunogen. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC, immunofluorescence, WB (1-5 µg/mL working concentration), Flow Cytometry (2?g/10^6 cells). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy-related protein 12; APG12-like; ATG12; APG12; APG12L
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
IHC and WB confirmed the specificity for ATG12. Human, rat
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Autophagy-related protein 16-1 (ATG16 L1) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA, ICC.
Background Info:
ATG16 L1 plays an essential role in autophagy. It is a homooligomer ant interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). APG16 L1 localises to the cytoplasm, in particularly to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. There are 5 named isoforms produced by alternative splicing. Genetic variation within ATG16 L1 are associated with susceptibility to inflammatory bowel disease type 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
N-terminal of human ATG16-L1 protein conjugated to Blue Carrier Protein has been used as the immunogen.
Applications:
ELISA,ICC,WB
Antibody Isotype:
Mixed
Application Details:
Non-reduced western blot: 1:100; IP: 1-50-1:100 (5-10 ?L/500uL lysate); ELISA: 1:2000-1:3000 direct detection only, recombinant immunogen.
Alternative Names:
Autophagy-related protein 16-1; APG16-like 1; ATG16L1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Cooney R et al (2009). NOD2 stimulation induces autophagy in dendritic cells influencing bacterial handling and antigen presentation. Nat. Med. Dec 6 2009 Scharl M. et al (2011). Protein tyrosine phosphatase nonreceptor type 2 regulates autophagosome formation in human intestinal cells. Inflamm Bowel Dis. 2011 Oct 10.
Specificity:
Detects human protein via non-reduced WB, and ELISA Human, rat. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Autophagy-related protein 3 (ATG3) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Background Info:
FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. SIMILARITY: Belongs to the ATG3 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a central region of the human ATG3 protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from ATG3 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).
Applications:
IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB, immunofluorescence. A dilution of 1:50 to 1:3000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy-related protein 3; APG3; APG3-like; hApg3; Protein PC3-96 ; ATG3; APG3; APG3L
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Ryningen A et al (2012) Inhibition of Mammalian target of rapamycin in human acute myeloid leukemia cells has diverse effects that depend on the environmental in vitro stress. Bone Marrow Res. 2012;2012:329061.
Specificity:
IHC, WB and ELISA confirmed the specificity for ATG3. A 37 kDa band, that corresponds to the molecular weight of ATG3, is detected via western blot analysis. Human, rat, mouse. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Autophagy-related protein 4 homolog B (APG4B) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 5 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. SIMILARITY: Belongs to the peptidase C54 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (CLERFFDSEDED) corresponding to the C-terminus of human ATG4B protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from of ATG4B protein in mouse and rat.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence, WB. A dilution of 1:100 to 1:2000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A. J. Smuder et al (2011) Exercise protects against doxorubicin-induced markers of autophagy signaling in skeletal muscle. J Appl Physiol. 2011 Jul 21
Specificity:
IHC, WB and ELISA confirmed the specificity for ATG4B. Human, rat. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Autophagy-related protein 4 homolog C (ATG4C) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). SIMILARITY: Belongs to the peptidase C54 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (CTVYNSDVIDKQ) corresponding to the C-terminal of human ATG4C protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from ATG4C protein in mouse and rat.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence and WB. A dilution of 1:100 to 1:2000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Smuder AJ et al. (2011) Exercise protects against doxorubicin-induced markers of autophagy signaling in skeletal muscle. J Appl Physiol (1985). 2011 Oct;111(4):1190-8.
Specificity:
IHC, ELISA and WB confirmed the specificity for ATG4C. Human, rat. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Autophagy-related protein 7 (ATG7) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Homodimer (By similarity). Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12. SIMILARITY: Belongs to the ATG7 family. In yeast, ATG7 appears to be required for fusion of peroxisomal and vaculuolar membranes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (DSTRDRTLDQQC) corresponding to the C-terminal of human APG7 protein conjugated to blue carrier protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from APG7 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
Non-reduced western blot : 1:100; IF: acetone or 3.7% PFA fixed cells (HJ Lim et al 2013); 1:100; Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Chiu-Wei Chen, et al (2012) Inhibition of autophagy as a therapeutic strategy of iron-induced brain injury after hemorrhage Autophagy, 8(10):1510. Chien W.S. et al (2011) Suppression of autophagy in rat liver at late stage of polymicrobial sepsis Shock. 2011 Jan 14 Lee J.E. et al (2011) Autophagy Regulates Embryonic Survival During Delayed Implantation Endocrinology. 2011 Mar 1. Ryningen A et al (2012) Inhibition of Mammalian target of rapamycin in human acute myeloid leukemia cells has diverse effects that depend on the environmental in vitro stress. Bone Marrow Res. 2012;2012:329061. Oh HA et al (2013) Uncovering a role for endocannabinoid signaling in autophagy in preimplantation mouse embryos. Mol Hum Reprod. 2013 Feb;19(2):93-101.
Specificity:
IF, WB and ELISA confirmed the specificity for ATG7. Human, rat. Other species not yet tested.
Storage:
Store lyophilized antibody at 2-8ºC. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Autophagy-related protein 9A (ATG9A) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
FUNCTION: Plays a role in autophagy. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein (Potential). ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. SIMILARITY: Belongs to the ATG9 family. IN YEAST: FUNCTION: Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Recruites ATG23 and ATG8 to the pre-autophagosomal structure. SUBUNIT: Interacts with ATG18, ATG2 and ATG23. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. Preautophagosomal structure; preautophagosomal structure membrane; multi-pass membrane protein. Note=Pre-autophagosomal and other perivacuolar punctate structures. The proper trafficking of ATG9 between the pre-autophagosomal structure and the other punctate structures requires ATG2, ATG18, ATG23, the ATG1-ATG13 complex and the phosphatidylinositol 3-kinase complex I. SIMILARITY: Belongs to the ATG9 family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (CWHRRESDESGE) corresponding to the C-terminal of human APG9A (APG9L1) protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from APG9A (APG9L1) protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence, WB. A dilution of 1:100 to 1:3000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Autophagy-related protein 9A; APG9-like 1; Autophagy-related protein 9; APG9A; ATG9A; APG9L1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Park YE et al (2009) Autophagic degradation of nuclear components in mammalian cells. Autophagy. 2009 Aug;5(6):795-804.
Specificity:
IHC, WB and ELISA confirmed the specificity for ATG9A (APG9L1) . Human, rat. Other species not yet tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
AUX1 (Auxin transporter protein 1) is a carrier protein involved in proton-driven auxin influx. Synthesized in developing leaves and transpported to tips. Involved in lateral root formation, trichoblast polarization and root hair elongation. Required for gravitropism and thigmotropism, especially in roots, by modulating responses to auxin, ethylene and cytokinins. Needed for ammonium-mediated root-growth inhibition. Alternative names: AUX1, AUXIN RESISTANT 1, WAV5, WAVY ROOTS 5, PIR1, MAP1, MODIFIER OF ARF7/NPH4 PHENOTYPES 1, Auxin influx carrier protein 1, Polar auxin transport inhibitor-resistant protein 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Goat
Species Reactivity:
Arabidopsis thaliana (recombinant AUX1)
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana AUX1. UniProt: Q96247, TAIR: AT2G38120
AUX1 (Auxin transporter protein 1) is a carrier protein involved in proton-driven auxin influx. Synthesized in developing leaves and transpported to tips. Involved in lateral root formation, trichoblast polarization and root hair elongation. Required for gravitropism and thigmotropism, especially in roots, by modulating responses to auxin, ethylene and cytokinins. Needed for ammonium-mediated root-growth inhibition. Alternative names: AUX1, AUXIN RESISTANT 1, WAV5, WAVY ROOTS 5, PIR1, MAP1, MODIFIER OF ARF7/NPH4 PHENOTYPES 1, Auxin influx carrier protein 1, Polar auxin transport inhibitor-resistant protein 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana (recombinant AUX1)
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana AUX1. UniProt: Q96247, TAIR: AT2G38120
B7H4 is a glycosylated transmembrane protein of the B7 family. It binds to activated T cells to moderate the T cell responses via cell cycle arrest in the T cell. Reverse signaling can induce either cell cycle arrest or apoptosis in the B7H4 expressing cell. B7H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC039
Antibody Isotype:
IgG2b
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
B7H4 is a glycosylated transmembrane protein of the B7 family. It binds to activated T cells to moderate the T cell responses via cell cycle arrest in the T cell. Reverse signaling can induce either cell cycle arrest or apoptosis in the B7H4 expressing cell. B7H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC039
Antibody Isotype:
IgG2b
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
B7H4 is a glycosylated transmembrane protein of the B7 family. It binds to activated T cells to moderate the T cell responses via cell cycle arrest in the T cell. Reverse signaling can induce either cell cycle arrest or apoptosis in the B7H4 expressing cell. B7H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC039
Antibody Isotype:
IgG2b
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
B7H4 is a glycosylated transmembrane protein of the B7 family. It binds to activated T cells to moderate the T cell responses via cell cycle arrest in the T cell. Reverse signaling can induce either cell cycle arrest or apoptosis in the B7H4 expressing cell. B7H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status.
B7H4 is a glycosylated transmembrane protein of the B7 family. It binds to activated T cells to moderate the T cell responses via cell cycle arrest in the T cell. Reverse signaling can induce either cell cycle arrest or apoptosis in the B7H4 expressing cell. B7H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status.
B7H4 is a glycosylated transmembrane protein of the B7 family. It binds to activated T cells to moderate the T cell responses via cell cycle arrest in the T cell. Reverse signaling can induce either cell cycle arrest or apoptosis in the B7H4 expressing cell. B7H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status.
BAK1 (Brassinosteroid insensitive 1-associated receptor kinase 1) controls expression of genes associated with innate immunity in the absence of pathogens or elicitors. Phosphorylated Brl1. Involved in programmed cell death (PCD). Subcellular localization: cell membrane. Alternative names: BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1, BAK1, BRI1-ASSOCIATED RECEPTOR KINASE, RKS10, RECEPTOR KINASES LIKE SERK 10, SERK3, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3, ELG, ELONGATED, ATSERK3, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3, ATBAK1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Solanum lycopersicum
Expected Species:
Thelungiella halophila Species of your interest not listed? Contact us
Extra Information on CE extraction buffer: CE buffer does not need to be made freshly everytime. Aliquots can be kept at -20 °C. Na2MoO4 and NaF are phosphatase inhibitors, included to prevent lose phosphorylation form our protein of interest during extraction. EDTA chelates metal ions and thus inhibits many enzymes which need metal ions as co-factors and inhibits the action of proteases. Protease inhibitor coctail is Sigma product number P 9599 which is used in dilution 1: 100.Antibody is reconizing AtBAK1-Myc in total extracts.
Xu et al. (2022) The Phloem Intercalated With Xylem-Correlated 3 Receptor-Like Kinase Constitutively Interacts With Brassinosteroid Insensitive 1-Associated Receptor Kinase 1 and Is Involved in Vascular Development in Arabidopsis. Front Plant Sci. 2022 Jan 11;12:706633. doi: 10.3389/fpls.2021.706633. PMID: 35087541; PMCID: PMC8786740.Kalischuk et al. (2022) Amplification of cell signaling and disease resistance by an immunity receptor Ve1Ve2 heterocomplex in plants. Commun Biol. 2022 May 25;5(1):497. doi: 10.1038/s42003-022-03439-0. PMID: 35614138; PMCID: PMC9132969.Katarzyna Parys et al (2021) Signatures of antagonistic pleiotropy in a bacterial flagellin epitope,Cell Host & Microbe,Volume 29, Issue 4,2021,Pages 620-634.e9,ISSN 1931-3128,https://doi.org/10.1016/j.chom.2021.02.008.(https://www.sciencedirect.com/science/article/pii/S1931312821000871)Zhang et al. (2019). An important role of L -fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity. New Phytol. 2018 Dec 15. doi: 10.1111/nph.15639.Hu et al. (2018). A group of receptor kinases are essential for CLAVATA signalling to maintain stem cell homeostasis. Nat Plants. 2018 Apr;4(4):205-211. doi: 10.1038/s41477-018-0123-z. (CoIP)
Special application note:
Antibodies bind to AtBAK1-Myc. They do not cross react with AtSOBIR.This product can be sold containing proclin if requested.
Beta amylase (EC 3.2.1.2) is an enzyme involved in the hydrolysis fo starch into sugars. Can play a minor role in the starch degradation and maltose metabolism in chloroplasts during the night.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
The mature length protein of Arabidopsis thaliana BAM1 overexpressed in E.coli, UniProt:Q9LIR6, TAIR: AT3G23920., lacking the transit peptide that is cleaved upon entry to the chloroplast. Recombinant protein had an N-terminal S-tag.
Beta amylase (EC 3.2.1.2.) catalyzes the hydrolysis of the second alfa-1,4 glycosidic bond. Alternative names 1,4-alfa-D-glucan maltohydrolase, glycogenase,saccharogen. amylase).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Kappaphycus alvarezii, Solanum tuberosum
Expected Species:
Arabidopsis thaliana, Glycine max, Physcomitrium patens, Populus trichocarpa, Ricinus communis, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
Beta amylase isolated and purified from sweet potato UniProt:Q94EU9
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Application Details:
1 : 1000-1 : 4000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG in PBS labelled with biotin.
Reconstitution:
For reconstitution add 1 ml of sterile water
Molecular Weight:
60 kDa
Selected references:
Usuldin et al. (2017). Molecular investigation of carrageenan production in Kappaphycus alvarezii in different culture conditions: a proteomic approach. ournal of Applied Phycology, August 2017, Volume 29, Issue 4, pp 1989–2001. (Kappaphycus alvarezii)
Special application note:
Biotin/IgG protein molar ratio (B/P) is approximately 6,6, No foreign proteins are added, Marker used for lebling is N-hydroxysuccinimidoBiotin
BAP1 (BRCA1 associated protein-1 or ubiquitin carboxy-terminal hydrolase) is a deubiquitinating enzyme that in humans is encoded by the BAP1 gene. BAP1 encodes an 80.4 kDa nuclear-localizing protein with a ubiquitin carboxy-terminal hydrolase (UCH) domain that gives BAP1 its deubiquitinase activity. In cancer, BAP1 can function both as a tumor suppressor and as a metastasis suppressor.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC039
Antibody Isotype:
IgG2b
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
BAP1 (BRCA1 associated protein-1 or ubiquitin carboxy-terminal hydrolase) is a deubiquitinating enzyme that in humans is encoded by the BAP1 gene. BAP1 encodes an 80.4 kDa nuclear-localizing protein with a ubiquitin carboxy-terminal hydrolase (UCH) domain that gives BAP1 its deubiquitinase activity. In cancer, BAP1 can function both as a tumor suppressor and as a metastasis suppressor.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC039
Antibody Isotype:
IgG2b
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
BAP1 (BRCA1 associated protein-1 or ubiquitin carboxy-terminal hydrolase) is a deubiquitinating enzyme that in humans is encoded by the BAP1 gene. BAP1 encodes an 80.4 kDa nuclear-localizing protein with a ubiquitin carboxy-terminal hydrolase (UCH) domain that gives BAP1 its deubiquitinase activity. In cancer, BAP1 can function both as a tumor suppressor and as a metastasis suppressor.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC039
Antibody Isotype:
IgG2b
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Phosphinothricin N-acetyltransferase (BAR) is an enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance, herbicide resitance gene. BAR (BASTA) gene is used as a selectable marker for genetic transformation of plants.Alternative names: PPT N-acetyltransferase, Phosphinothricin-resistance protein.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
BAR (BASTA)
Expected Species:
Streptomyces viridochromogenesSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from position 170-183 of Phosphinothricin N-acetyltransferase (BAR or BASTA), UniProt: P16426
BAR (BASTA) gene is a selectable marker of plant genetic transformation, Nada (2016). Novel recombinant binary vectors harboring Basta (bar) gene as a plant selectable marker for genetic transformation of plants. Physiol Mol Biol Plants. 2016 Apr; 22(2): 241–251.
Application Details:
1 : 1000 - 1: 5000 (WB)
Purity:
Antigen affinity purified serum, in PBS pH 7.4
Reconstitution:
For reconstitution add 50 l, of sterile or deionized water.
Molecular Weight:
20.6 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
To be added when available, antibody available in May 2023.
Phosphinothricin N-acetyltransferase (BAR) is an enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance, herbicide resitance gene. BAR (BASTA) gene is used as a selectable marker for genetic transformation of plants.Alternative names: PPT N-acetyltransferase, Phosphinothricin-resistance protein.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
BAR (BASTA)
Expected Species:
Streptomyces viridochromogenesSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from position 36-50 of Phosphinothricin N-acetyltransferase (BAR or BASTA), UniProt: P16426
BAR (BASTA) gene is a selectable marker of plant genetic transformation, Nada (2016). Novel recombinant binary vectors harboring Basta (bar) gene as a plant selectable marker for genetic transformation of plants. Physiol Mol Biol Plants. 2016 Apr; 22(2): 241–251.
Application Details:
1 : 1000 (WB)
Purity:
Antigen affinity purified serum, in PBS pH 7.4
Reconstitution:
For reconstitution add 50 l, of sterile or deionized water.
Molecular Weight:
20.6 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
To be added when available, antibody available in May 2023.
Fibroblast growth factors (FGFs), a heparin binding growth factor, exhibit widespread mitogenic and neurotrophic activities in a variety of different cells including mesenchymal, neuroectodermal and endothelial cells. aFGF (FGF-1) and bFGF (FGF-2) are present in relatively high levels in CNS. aFGF is expressed by a subset of neuronal populations, while bFGF is expressed by astrocytes, both lack signal peptides. Human bFGF is a 17.2 kDa protein containing 155 amino acid residues. FUNCTION: The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. SUBUNIT: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2. MISCELLANEOUS: This protein binds heparin more strongly than does aFGF. SIMILARITY: Belongs to the heparin-binding growth factors family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human basic FGF
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC (frozen), WB. Recommended to be used at a dilution of 1: 1000 to 1:2000 for both applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Lee HJ et al (2010) Effects of sevoflurane on collagen production and growth factor expression in rats with an excision wound. Acta Anaesthesiol Scand. 2010 Aug;54(7):885-93.
Specificity:
A high level of specificity for bFGF was shown by immunohistochemistry for this antiserum. This antibody is known to react with human, mouse and rat basic FGF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
BC2 tag is a short and inert peptide-tag for protein studies.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Protein of interest overexpressed wth BC2-tag
Immunogen:
KLH-conjugated BC2 tag PDRKAAVSHWQQ derived from beta catenin.
BC2 tag is a short and inert peptide-tag for protein studies.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
Protein of interest overexpressed wth BC2-tag
Immunogen:
KLH-conjugated BC2 tag PDRKAAVSHWQQ derived from beta catenin.
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight 488.
Selected references:
To be added when available. Antibody released in May 2023.
Special application note:
This antibody is suitable for detection of recombinant proteins with BC2 tag.DyLight 488 Amax = 493 nm, Emax = 519 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
BC2 tag is a short and inert peptide-tag for protein studies.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
Protein of interest overexpressed wth BC2-tag
Immunogen:
KLH-conjugated BC2 tag PDRKAAVSHWQQ derived from beta catenin.
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight 594.
Selected references:
To be added when available. Antibody released in May 2023.
Special application note:
This antibody is suitable for detection of recombinant proteins with BC2 tag.DyLight 594 has Amax = 593 nm, Emax = 618 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
BC2 tag is a short and inert peptide-tag for protein studies.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
Protein of interest overexpressed wth BC2-tag
Immunogen:
KLH-conjugated BC2 tag PDRKAAVSHWQQ derived from beta catenin.
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight 650.
Selected references:
To be added when available. Antibody released in May 2023.
Special application note:
This antibody is suitable for detection of recombinant proteins with BC2 tag.DyLight 650 has Amax = 652 nm, Emax = 672 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
The BCA-225 [IHC225] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC225
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Breast Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The BCA-225 [IHC225] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC225
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Breast Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The BCA-225 [IHC225] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC225
GMDN Code:
?
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Breast Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
B-Cell Lymphoma 2 (BCL2) is involved in regulation of cell apoptosis by controlling mitochondrial permeability and release of cytochrome c. It also has critical roles in normal cell physiology related to neuronal activity, autophagy, calcium handling, mitochondrial dynamics, and energetics. BCL2 overexpression has been shown to promote cell survival by suppressing apoptosis, and is found to be correlated with poor disease prognosis in breast, prostate, ovarian, endometrial, and colon cancers. In follicular lymphoma, Anti-BCL2 reacts negatively with germinal centers and positively with neoplastic follicles. In lymphoid lesions, BCL2 staining is useful for distinguishing reactive and neoplastic follicular proliferations, and for identifying minimal residual disease in the bone marrow of follicular lymphoma patients. BCL2 is now a useful target of human cancer therapy.
B-Cell Lymphoma 2 (BCL2) is involved in regulation of cell apoptosis by controlling mitochondrial permeability and release of cytochrome c. It also has critical roles in normal cell physiology related to neuronal activity, autophagy, calcium handling, mitochondrial dynamics, and energetics. BCL2 overexpression has been shown to promote cell survival by suppressing apoptosis, and is found to be correlated with poor disease prognosis in breast, prostate, ovarian, endometrial, and colon cancers. In follicular lymphoma, Anti-BCL2 reacts negatively with germinal centers and positively with neoplastic follicles. In lymphoid lesions, BCL2 staining is useful for distinguishing reactive and neoplastic follicular proliferations, and for identifying minimal residual disease in the bone marrow of follicular lymphoma patients. BCL2 is now a useful target of human cancer therapy.
B-Cell Lymphoma 2 (BCL2) is involved in regulation of cell apoptosis by controlling mitochondrial permeability and release of cytochrome c. It also has critical roles in normal cell physiology related to neuronal activity, autophagy, calcium handling, mitochondrial dynamics, and energetics. BCL2 overexpression has been shown to promote cell survival by suppressing apoptosis, and is found to be correlated with poor disease prognosis in breast, prostate, ovarian, endometrial, and colon cancers. In follicular lymphoma, Anti-BCL2 reacts negatively with germinal centers and positively with neoplastic follicles. In lymphoid lesions, BCL2 staining is useful for distinguishing reactive and neoplastic follicular proliferations, and for identifying minimal residual disease in the bone marrow of follicular lymphoma patients. BCL2 is now a useful target of human cancer therapy.
B-Cell Lymphoma 6 (BCL6) is a zinc finger transcription factor. BCL6 expression is seen in follicular lymphomas, Burkitt's lymphoma, angioimmunoblastic T-cell lymphoma, and nodular lymphocyte-predominant Hodgkin's lymphoma. Together with BCL2, BCL6 is often used to distinguish neoplastic follicles from those in benign hyperplasia, and to aid in the classification of mantle cell lymphomas and nodular lymphocyte-predominant Hodgkin's lymphoma.
B-Cell Lymphoma 6 (BCL6) is a zinc finger transcription factor. BCL6 expression is seen in follicular lymphomas, Burkitt's lymphoma, angioimmunoblastic T-cell lymphoma, and nodular lymphocyte-predominant Hodgkin's lymphoma. Together with BCL2, BCL6 is often used to distinguish neoplastic follicles from those in benign hyperplasia, and to aid in the classification of mantle cell lymphomas and nodular lymphocyte-predominant Hodgkin's lymphoma.
B-Cell Lymphoma 6 (BCL6) is a zinc finger transcription factor. BCL6 expression is seen in follicular lymphomas, Burkitt's lymphoma, angioimmunoblastic T-cell lymphoma, and nodular lymphocyte-predominant Hodgkin's lymphoma. Together with BCL2, BCL6 is often used to distinguish neoplastic follicles from those in benign hyperplasia, and to aid in the classification of mantle cell lymphomas and nodular lymphocyte-predominant Hodgkin's lymphoma.
Rabbit anti-Beclin-1 Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Beclin-1 plays a central role in autophagy. It may also play a role in antiviral host defense. It is ubiquitously expressed and it localises to the cytoplasm, golgi apparatus membrane, and in dendrites and cell bodies of cerbellar Purkinje cells. The unprocessed precursor has a length of 450 amino acids and an estimated molecular weight of 51.89 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
C-terminal of human Beclin-1 conjugated to Blue Carrier Protein.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:100 to 1:2000 dilution is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Coiled-coil myosin-like BCL2-interacting protein; Protein GT197; APG 6; BECN1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specific for Beclin-1. Human, other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
beta-1,4-glucosidase (hyperthermophilic enzyme) which is releasing glucose from soluble soluble glucooligomers, with a preference for longer oligomers; acts more readily on cellotetraose than on cellobiose. Displays similar activities towards the disaccharides lactose and cellobiose.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Thermotoga neapolitana
Expected Species:
Thermotoga neapolitana
Immunogen:
His-tagged, recombinant 1,4-beta-D-glucan glucohydrolase of Thermotoga neapolitana, overexpressed in E.coli,, UniProt: B9K7M5
Beta-adrenergic receptors are multi-pass membrane proteins that belong to the G-protein coupled receptor 1 family. Their function is to mediate the catecholamine-induced activation of adenylate cyclase through the action of G-proteins (ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide from mouse beta 3 Adrenergic Receptor (386-400 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Beta-Actin is a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism. Actin is expressed at high levels in almost all tissues and cell lines. This makes it ideal to use as a loading control in Western blot.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store at -20 °C for 3 years or more, Reconstitute with distilled water to desired concentration before use, Aliquot to avoid repeated freeze-thaw cycles, Store aliquots at 4°Cfor several days to weeks
Host Animal:
Mouse
Species Reactivity:
Chicken, human, mouse, rabbit, rat
Immunogen:
Synthetic peptide derived from the N-terminal of the human beta-actin
Applications:
Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Beta-Actin is a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism. Actin is expressed at high levels in almost all tissues and cell lines. This makes it ideal to use as a loading control in Western blot.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Synthetic peptide derived from the N-terminal of the beta-actin UniProt: P60709
Rabbit anti-Beta Amyloid Polyclonal Antibody (Unconjugated), suitable for ELISA.
Background Info:
The beta Amyloid peptide is derived from the cleavage of the Amyloid precursor protein and varies in length from 39 to 43 amino acids. Beta amyloid peptides are the major constituents of the plaques and tangles that occur in Alzheimer's disease.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide (DAEFRHDSGYEVHH) conjugated to bovine serum albumin (BSA) corresponding to amino acid sequence 1-14 of mature human beta amyloid.
Applications:
ELISA
Antibody Isotype:
Mixed
Application Details:
ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human beta amyloid, cross reactivity to APP has not bee tested.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Beta-CA1, beta-CA2 is a low-CO2-induced mitochondrial carbonic anhydrase. that catalyze the reversible interconversion of carbon dioxide and water to bicarbonate and protons. Localised into mitochondrial stroma.Alternative name: MtCA.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Chlamydomonas reinhardtii
Immunogen:
recombinant Chlamydomonas reinhardtii mitochondrial CA, as described in Villand et al. 1997. Accession number Q39590 and Q39589
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022). https://doi.org/10.1038/s41586-022-04662-9Kuken et al. (2018). Effects of microcompartmentation on flux distribution and metabolic pools in Chlamydomonas reinhardtii chloroplasts. Elife. 2018 Oct 11;7. pii: e37960. doi: 10.7554/eLife.37960.Muranaka et al. (2015). TEF30 interacts with photosystem II monomers and is involved in the repair of photodamaged photosystem II in Chlamydomonas reinhardtii. Plant Physiol. 2015 Dec 7. pii: pp.01458.2015.Tirumani et al. (2014). Regulation of CCM genes in Chlamydomonas reinhardtii during conditions of light-dark cycles in synchronous cultures. Plant Mol Biol. 2014 Mar 4.Renberg et al. (2010). A Metabolomic Approach to Study Major Metabolite Changes during Acclimation to Limiting CO2 in Chlamydomonas reinhardtii. Plant physiol. 154: 187-196.
Special application note:
Antibody is recognizing both isoforms, beta- CA1 and beta-CA2 and can be used as mitochondrial marker for low carbon dioxide grown cells of Chlamydomonas reinhardtii
BetaCA1 (Beta carbonic anhydrase 1) (chloroplastic) - is a zinc metalloenzyme that interconvert CO2 and HCO3 (-). Alternative names: ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3, ATBCA1, ATSABP3, BETA CARBONIC ANHYDRASE 1, CA1, CARBONIC ANHYDRASE 1, SABP3, SALICYLIC ACID-BINDING PROTEIN 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Solanum lycopersicum Q5NE20 Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide, derived in the part C-terminus of BetaCA1 of Arabidopsis thaliana, UniProt: P27140, TAIR: At3g01500
Extraction method – Grind 50 mg of leaf tissue in a sterile microcentrifuge tube using a sterile plastic pestle. Add 132μL of Protein Extraction Buffer (1x TE, 1.2 %SDS, 2.7% sucrose, 7.5 μg mL-1 bromophenol blue) to the ground leaf tissue. Vortex the sample and keep on ice for 15 mins. Centrifuge at 14,000 rpm for five minutes using a benchtop centrifuge. Collect the supernatant and in a new sterile 0.5 ml microcentrifuge tube and discard the pellet.This antibody does not recognize betaCA2.
Application Details:
1 : 20 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l, of sterile water
Molecular Weight:
37,5 | 25,3 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
DiMario et al. (2016). The Cytoplasmic Carbonic Anhydrases βCA2 and βCA4 Are Required for Optimal Plant Growth at Low CO2. Plant Physiol. 2016 May;171(1):280-93. doi: 10.1104/pp.15.01990.
Rabbit anti-Beta casein protein Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Beta-casein has an important role in determination of the surface properties of the casein micelles. it is cleaved into 3 chains (casoparan, casohypotensin and antioxidant peptide). Casoparan acts as a macrophage activator, increasing the phagocytic activity of macrophages and peroxide release from macrophages. It also acts as a bradykinin-potentiating peptide. Casohypotensin acts as a bradykinin-potentiating peptide and induces hypotension in rats. Acts as a strong competitive inhibitor of endo-oligopeptidase A. Antioxidant peptide has antioxidant activity (ref: uniprot.org.).<br /><br />Bovine milk contains two types of beta-casein protein, A2 or A1. Recent studies have shown that milk containing the A1 beta casein protein can contribute to issues including gastrointestinal discomfort after ingestion. There is some evidence of a link between ingestion of A1 beta casein protein and the development of Type 1 diabetes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS, pH 7.4, with 0.02% sodium azide as preservative. Refer to the product label for antibody concentration.
Host Animal:
Rabbit
Species Reactivity:
Bovine
Immunogen:
Bovine beta-casein peptides
Applications:
ELISA,WB
Antibody Isotype:
IgG
Application Details:
ELISA and western blotting (1:5,000 - 1: 20,000). Do not use skim milk for blocking and dilution of assay reagents, BSA is recommended. Biosensis recommends that optimal working dilutions should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Physical State:
Liquid.
Product references:
Indyk HE et al. (2021) The determination of intact ?-casein in milk products by biosensor immunoassay. J. Food Compos. Anal. 101:103946 Application: Immunoassay.
Specificity:
Bovine beta-casein. Detects both A1 and A2 beta-casein isoforms.
Storage:
Spin vial briefly before opening and centrifuge to remove any insoluble material. After opening maintain at -20°C in undiluted aliquots for up to 6 months. For short-term storage, keep aliquot at 2-8°C for up to one week. Avoid repeated freeze-thaw cycles.
β-Catenin is a cytoplasmic protein with a dual role in cell-cell adhesion and gene expression. It is normally present in the submembranous regions of the cell, and nuclear accumulation of β-Catenin has been found to occur as a result of gene mutations. This accumulation is useful in identifying desmoid tumours (fibromatosis) in the abdomen and breast, and is therefore useful in differentiating other cell neoplasms in these regions.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC516
Antibody Isotype:
IgG1
GMDN Code:
56911
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Fibromatosis of Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
β-Catenin is a cytoplasmic protein with a dual role in cell-cell adhesion and gene expression. It is normally present in the submembranous regions of the cell, and nuclear accumulation of β-Catenin has been found to occur as a result of gene mutations. This accumulation is useful in identifying desmoid tumours (fibromatosis) in the abdomen and breast, and is therefore useful in differentiating other cell neoplasms in these regions.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC516
Antibody Isotype:
IgG1
GMDN Code:
56911
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Fibromatosis of Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
β-Catenin is a cytoplasmic protein with a dual role in cell-cell adhesion and gene expression. It is normally present in the submembranous regions of the cell, and nuclear accumulation of β-Catenin has been found to occur as a result of gene mutations. This accumulation is useful in identifying desmoid tumours (fibromatosis) in the abdomen and breast, and is therefore useful in differentiating other cell neoplasms in these regions.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC516
Antibody Isotype:
IgG1
GMDN Code:
56911
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Fibromatosis of Breast
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The Beta-Endorphin Antibody was raised to synthetic human beta endorphin coupled to KLH with carbodiimide. The antibody produces a strong indirect immunofluorescent staining at a 1/200 - 1/400 dilution and a 4+ biotin-streptavidin/HRP staining at a 1/1000 - 1/2000 dilution in rat anterior pituitary. Staining is completely eliminated by pretreatment of the diluted antibody with 10-6 M of Ã-Endorphin. Pre-adsorption of the diluted antibody with 10-6M of the following substances had no effect on -Endorphin labeling: methionine enkephalin, leucine enkaphalin, dynorphin A, dynorphin B, gamma-endorphin, alpha-endorphin, ACTH and alpha-melanocyte stimulating hormone.
Human beta-endorphin is a 31 amino acid peptide cleaved from the precursor pro-opiomelanocortin (POMC). It is an endogenous opioid peptide neurotransmitter that interacts with opioid receptors.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic human beta-endorphin peptide (1-31 aa) conjugated to BSA.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. For radioimmunoassay (RIA), a working dilution of 1:10,000 is recommended. Sensitivity - 120 pg/sample; IC50-1 ng/ sample. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
-endorphin; Pro-opiomelanocortin; POMC;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed by RIA against the synthetic peptide. Human; mouse; rat. Beta-endorphin is highly conserved so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Met-enkephalin 0.03%; with Leu-enkephalin 0.02%; with beta-lipotropin 0.34%.
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
Beta-Galactosidase is an enzyme involved in hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. Alternative names: Beta-gal, Lactase
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Escherichia coli
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
beta-Galactosidase is isolated and purified from Escherichia coli, UniProt: P00722
Applications:
Dot blot (Dot), ELISA (ELISA), Immunocytochemistry (IHC), Western blot (WB)
Beta-Galactosidase is an enzyme involved in hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. Alternative names: Beta-gal, Lactase
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Escherichia coli
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
beta-Galactosidase is isolated and purified from Escherichia coli, UniProt: P00722
Applications:
Dot blot (Dot), ELISA (ELISA), Indirect immunofluorescence (indirect IF), Western blot (WB)
Beta-Galactosidase is an enzyme (EC:3.2.1.23) involved in hydrolysis of terminal non-reducing beta-D-galactose residues into beta-D-galactosides. The protein is encoded by lacZ gene.Alternative names: Beta-gal, Lactase, GalB
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid at 1 mg/ml.
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Escherichia coli, GalB-tagged fusion proteins
Immunogen:
beta-Galactosidase purified from E. coli. UniProt: P00722
Human beta-Lipotropin is a 93 amino acid polypeptide that is cleaved from carboxy-terminal fragment of the precursor pro-opiomelanocortin (POMC). It stimulates melanocytes to produce melanin, and can also be cleaved into smaller peptides including opioid peptides: gamma-lipotropin, alpha-MSH, beta-MSH, gamma-MSH, alpha-endorphin, beta-endorphin, gamma-endorphin and met-enkephalin
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic peptide (179-267 aa) of human beta-Lipotropin conjugated to thyroglobulin was used as the immunogen.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human; mouse; rat. Beta-Lipotropin is highly conserved so cross-reactivity with other species is expected. Cross-reactivity with other opioid peptides is as follows: with Leu-enkephalin 0.01%; with Met-Enkephalin 0.01%; with beta-endorphin 0.01%
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent.Avoid freeze-thaw cycles
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A dilution of 1:1000-1:5000 is recommended for IHC, western blot and immunoblot; 1:15000 for ELISA; for inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Mulhall J.P. et al (2008) J Sex Med. May;5(5):1126-36.
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF bot not bovine NGF.
Storage:
Store lyophilized antibody at 2-8ºC. After reconstitution keep aliquots at -20°C to -80ºC for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.2-7.6 without preservatives
Host Animal:
Rabbit
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antibody was tested on cultured sensory neurons supported by 100 ng/mL of purified mouse beta NGF. Be advised that 2 µg/mL will neutralize 100 ng/mL of mouse NGF. The higher 10 µg/mL is only recommended if the concentration of NGF being used is higher than 100 ng/mL such as the 200 or 500ng/mL that is occasionally used in some culture systems. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Laurina Z. et al (2009) Growth factors/cytokines/defensins and apoptosis in periodontal pathologies. Stomatologija. 2009;11(2):48-54. Lee H.W. et al (2007) Expression of nerve growth factor is upregulated in the rat thymic epithelial cells during thymus regeneration following acute thymic involution. Regul Pept. 2007 Jun 7;141(1-3):86-95
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
Storage:
Store lyophilized antibody at 2-8ºC. After reconstitution keep aliquots at -20°C to -80ºC for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A dilution of 1:1000-1:5000 is recommended for IHC, western blot and immunoblot; 1:15000 for ELISA; for inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Feron F et al (2008) Neurotrophin expression in the adult olfactory epithelium. Brain Res. 1196:13-21 Application: IHC ; Species: Rat
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Mouse anti-Beta-synuclein Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease (Ref: uniprot.org).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
C-terminal peptide of human ?-synuclein (EPEGESYEDPPQEEYQEYEPEA) coupled to KLH.
Applications:
IHC-Frozen,WB
Clone number:
6A10
Antibody Isotype:
IgG1
Application Details:
Western blotting (1:1,000) and Immunohistochemistry (frozen sections, 1:500-1:1,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-synuclein, ?-synuclein
Biosensis Brand:
Biosensis®
Cellular Localisation:
Intracellular, cytosolic.
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Immunogen epitope location:
C-terminus.
Immunogen length:
22 amino acids.
Physical State:
Solid.
Specificity:
Specific for ?-synuclein, does not cross-react with ?- or ?-synuclein.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Product Validation Info:
Validated by western blotting and immunohistochemical procedures.
Purification:
Affinity-purified from conditioned medium using the immunogen.
Rabbit anti-Beta-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease (Ref: uniprot.org).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Full-length human recombinant ?-synuclein protein expressed in and purified from E. Coli.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
Western blotting (1:1,000-1:2,000) and Immunohistochemistry (frozen sections, 1:1,000-1:2,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-synuclein, ?-synuclein
Biosensis Brand:
Biosensis®
Cellular Localisation:
Intracellular, cytosolic.
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Immunogen length:
Full-length recombinant protein.
Physical State:
Solid.
Specificity:
Specific for ?-synuclein, does not cross-react with ?- or ?-synuclein.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Product Validation Info:
Validated by western blotting and immunohistochemical procedures.
Purification:
Affinity-purified from rabbit serum using the immunogen.
Rabbit anti-Beta-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Beta-synuclein is a non-amyloid component of senile plaques found in Alzheimer disease. It could act as a regulator of SNCA aggregation. It protects nerurons from staurosporine and 6 hydroxy dopamine -stimulated capspase activation in a p53-dependent manner. It localises to the cytoplasm and it is predominantly expressed in the brain where it is most concentrated in presynaptic nerve terminals. This protein is phosphorylated. This protein is also associated with the disease Brain iron accumulation type 1 (NBIA1).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (IEPLMEPEGSYEDPPQE) of human beta synuclein protein (aa: 108-125) conjugated to diptheria toxid has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB , immunoblot. A dilution of 1:500 to 1:2000 is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
SNCB
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Product references:
Israeli E. and Sharon R. (2009) Beta-synuclein occurs in vivo in lipid-associated oligomers and forms hetero-oligomers with alpha-synuclein J Neurochem. 2009 Jan;108(2):465-74
Specificity:
Less than 0.1% cross reactivity to human alpha synuclein This antiserum is known to react with human and rat beta synuclein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Rabbit anti-Beta-synuclein Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Beta-synuclein is a soluble cytoplasmic protein associated with synaptic vesicles and a member of the synuclein family. Mutations in alpha-synuclein cause early onset Parkinson's disease. Expression of beta synuclein may modulate alpha-synuclein aggregation found in Parkinson's disease.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4. Contains no preservative.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide (AQEAAEEPLIEPLME-C) corresponding to human _-synuclein [99-113] in the C-terminal domain conjugated via additional C-terminal Cys to Diphtheria toxoid.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
WB and IHC. A dilution of 1:500 to 1:1,000 is recommended for Western blot. _-synuclein is a soluble protein of 134 amino acids and detected with 17 kDa mobility by western blotting. By IHC the antibody detects synaptic sites in human brain formaldehyde-treated frozen tissue. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Confirmed by WB using soluble mouse and human brain extracts, reactivity for major product diminished by peptide absorption. Does not detect alpha-synuclein as tested with recombinant protein and does not react with Lewy bodies in human Dementia with Lewy Bodies or Parkinson's disease brain tissue sections.
Storage:
Lyophilized at 2-8°C. After reconstitution, store at -20°C in undiluted aliquots for up to 6 months. The antibody may be stored short term at 2-2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.
Sheep anti-Beta-synuclein Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Beta-synuclein is a non-amyloid component of senile plaques found in Alzheimer disease. It could act as a regulator of SNCA aggregation. It protects neurons from staurosporine and 6 hydroxy dopamine -stimulated capspase activation in a p53-dependent manner. It localises to the cytoplasm and it is predominantly expressed in the brain where it is most concentrated in presynaptic nerve terminals. This protein is phosphorylated. This protein is also associated with the disease Brain iron accumulation type 1 (NBIA1).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Affinity purified and dialysed against PBS. Contains 0.02% sodium azide.
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (IEPLMEPEGSYEDPPQE) of human beta synuclein protein (aa: 108-125) conjugated to diptheria toxid has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
IgG
Application Details:
IHC. A working concentration of 1 µg/mL is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
SNCB
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Known to be specific for beta synuclein. This antiserum is known to react with beta synuclein of human, rat and other rodents.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Sheep anti-Beta-synuclein Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Beta-synuclein is a non-amyloid component of senile plaques found in Alzheimer disease. It could act as a regulator of SNCA aggregation. It protects nerurons from staurosporine and 6 hydroxy dopamine -stimulated capspase activation in a p53-dependent manner. It localises to the cytoplasm and it is predominantly expressed in the brain where it is most concentrated in presynaptic nerve terminals. This protein is phosphorylated. This protein is also associated with the disease Brain iron accumulation type 1 (NBIA1).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (IEPLMEPEGSYEDPPQE) as part of human beta synuclein protein (aa: 108-125) conjugated to diphteria toxid has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:3000 to 1:8000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
SNCB
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Known to be specific for beta synuclein. This antiserum is know to react with human, rat and other rodent beta synuclein.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Beta-tubulin is a conserved protein involved in cell motility, structure and integrity, tissue development and the development of organism. Tubulin is expressed at high levels in almost all tissues and cell lines. This makes it ideal to use as a loading control in Western blot.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store at -20 °C for 3 years or more, Reconstitute with distilled water to desired concentration before use, Aliquot to avoid repeated freeze-thaw cycles, Store aliquots at 4°Cfor several days to weeks
Host Animal:
Mouse
Species Reactivity:
Chicken, human, monkey, mouse, rat, rabbit
Immunogen:
Synthetic peptide derived from the N-terminal of the human beta-tubulin
Applications:
Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Tubulin is the major constituent of microtubules. There are three members (alpha, beta and gamma) and two subtypes in the tubulin family. Of these members, beta tubulin (449 aa, 51 kDa) is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly during cell cycle.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Schizosaccharomyces pombe
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated C-terminal peptide C-YEIEEEKEPLEY-OH from beta tubulin of Schizosaccharomyces pombe, UniProt: P05219
Applications:
ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
Immunogen affinity purified serum in PBS and 50 % glycerol, filter sterilized.
Molecular Weight:
51 | 45 kDa
Selected references:
Fedyanina et al. (2009) Tubulin heterodimers remain functional for one cell cycle after the inactivation of tubulin-folding cofactor D in fission yeast cells. Yeast. 2009 Apr;26(4):235-47. doi: 10.1002/yea.1663. PMID: 19330768; PMCID: PMC5705012.
Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at 2-8 C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
BFP, GFP, YFP
Immunogen:
Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
Immunoglobulin Protein A purified in a 10 mM ammonium bicarbonate buffer, with 2 mg of BSA.
Reconstitution:
Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%). Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 •Additional sodium azide ( up to 0.05%) is recommended for long term storage. •For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 μl buffer.
BG1 (Beta-glucosidase 1) hydrolyzes abscisic acid glucose ester (ABA-GE) which represents the predominant form of conjugated ABA (biologically inactive). No activity with beta-D-glucopyranosyl zeatin. The hydrolysis of ABA-GE in the endoplasmic reticulum (ER) forms free ABA and contributes to increase its cellular levels under dehydration conditions. ABA-GE hydrolyzing activity is enhanced by dehydration stress-induced polymerization into higher molecular weight forms. The ABA produced by BGLU18 contributes to the initiation of intracellular signaling as well as the increase in the extracellular ABA level. Localised to ER lumen.Alternative names: AtBG1, Beta-glucosidase 18, Beta-glucosidase homolog 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml.
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Purified recombinant BG1 of Arabidopsis thaliana, residues 27-528 with a His6-thioredoxin tagged, UniProt: Q9SE50, TAIR: At1g52400
Applications:
Immunolocalization (IL) using electron microscopy, Western blot (WB)
BG8 Lewis y, also known as Lewis<sup>y</sup> Blood Antigen or simply BG8, is a blood group antigen that has been identified in many studies as a potential marker for differentiation between pulmonary adenocarcinoma (PACA) and epithelioid mesothelioma (EM). It has been reported that sensitivity of non-mesothelial antigens for adenocarcinoma is organ-dependent. When attempting to differentiate epithelioid mesothelioma from adenocarcinoma, BG8 Lewis<sup>y</sup> performed at a sensitivity of 98% in the breast cancer group, and 100% in the lung cancer group.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC517
Antibody Isotype:
IgG1, kappa
GMDN Code:
63793
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Lung Adenocarcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
BG8 Lewis y, also known as Lewis<sup>y</sup> Blood Antigen or simply BG8, is a blood group antigen that has been identified in many studies as a potential marker for differentiation between pulmonary adenocarcinoma (PACA) and epithelioid mesothelioma (EM). It has been reported that sensitivity of non-mesothelial antigens for adenocarcinoma is organ-dependent. When attempting to differentiate epithelioid mesothelioma from adenocarcinoma, BG8 Lewis<sup>y</sup> performed at a sensitivity of 98% in the breast cancer group, and 100% in the lung cancer group.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC517
Antibody Isotype:
IgG1, kappa
GMDN Code:
63793
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Lung Adenocarcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
BG8 Lewis y, also known as Lewis<sup>y</sup> Blood Antigen or simply BG8, is a blood group antigen that has been identified in many studies as a potential marker for differentiation between pulmonary adenocarcinoma (PACA) and epithelioid mesothelioma (EM). It has been reported that sensitivity of non-mesothelial antigens for adenocarcinoma is organ-dependent. When attempting to differentiate epithelioid mesothelioma from adenocarcinoma, BG8 Lewis<sup>y</sup> performed at a sensitivity of 98% in the breast cancer group, and 100% in the lung cancer group.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC517
Antibody Isotype:
IgG1, kappa
GMDN Code:
63793
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Lung Adenocarcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
BIK1 (Botrytis-induced kinase 1) belongs to the protein kinase superfamily. BIK1 is a crucial component of host response signaling required to activate the resistance responses to Botrytis and A. brassicicola infection.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Noccaea caerulescensSpecies of your interest not listed? Contact us
Ngou et al. (2021) Mutual potentiation of plant immunity by cell-surface and intracellular receptors. Nature. 2021 Mar 10. doi: 10.1038/s41586-021-03315-7. Epub ahead of print. PMID: 33692545.Wang et al. (2021) Arabidopsis PUB2 and PUB4 connect signaling components of pattern-triggered immunity. New Phytol. 2021 Dec 17. doi: 10.1111/nph.17922. Epub ahead of print. PMID: 34918346
Special application note:
This product can be sold containing ProClin if requested
Bim (Bcl2-interacting mediator) is a pro-apoptotic protein of BH3 domain-only subgroup of the Bcl2 family. It has important roles in initiation of apoptosis in response to many death stimuli. Bim is an important regulator of B and T cell negative selection and is also an essential regulator of T cell apoptosis during termination of an immune response. Bim is constitutively expressed in many cell types but it is maintained in an inactive form through binding to the microtubule-associated dynein motor complex._x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
The Armenian hamster antibody Ham151-149 reacts with mouse Bim, a 19-24 kDa pro-apoptotic protein (Bcl-2 family) which regulates immunological responses._x000D_
BIN2 (Brasinosteroid insensitive 2) plays an important role in regulating brassinosteroid signalling which is important for plant growth. It is also implied to mediate auxing signalling pathway. Phosphorylation of BZR1 and BZR2/BES2 by BIN2 increases degradation of these proteins by the proteasome.Alternative names:ATSK21, DWARF 12, DWF12, SHAGGY-LIKE KINASE 21, SK21, UCU1, ULTRACURVATA 1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Hu et al. (2023) Spatiotemporal formation of the large vacuole regulated by the BIN2-VLG module is required for female gametophyte development in Arabidopsis [published online ahead of print, 2023 Jan 17]. Plant Cell. 2023;koad007. doi:10.1093/plcell/koad007
Biotin has a high affinity to avidin or streptavidin and is there used as a common tag.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Lyophilized powder is stable for a minimum of 2 years at -20 °C. Reconstitute in distilled water before use. Store reconstituted antibodies at 4°Cfor up to a month and make aliquots for extended storage.
Host Animal:
Mouse
Species Reactivity:
Antibody detects free biotin and biotin bound on a carrier protein.
Immunogen:
Biotin
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). Alternative name: AtBP2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4 C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Piea sitchensis, Populus trichocarpa, Spinacia oleracea, Zea mays Species of your interest not listed? Contact us
Protein or membrane sample should be treated at 70 C for 10 min before loading on the gel
Application Details:
1 : 50-1 : 1000 (IF), 1 : 2000 (WB)
Purity:
Immunogen affinity purified total IgY. in PBS pH 7.4.
Molecular Weight:
73,5 | 80 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Bennett et al. (2014). Plasma Membrane-Targeted PIN Proteins Drive Shoot Development in a Moss. Curr Biol. 2014 Dec 1;24(23):2776-85. doi: 10.1016/j.cub.2014.09.054. Epub 2014 Nov 13.
Special application note:
Antibody solution contains 0,02% sodium azide as preservative
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER, BiP protein is abundant under all growth conditions, but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER, BiP protein is abundant under all growth conditions, but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER, BiP protein is abundant under all growth conditions, but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). Alternative name: AtBP2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Picea sitchensis, Populus trichocarpa, Physcomitrium patens, Spinacia oleracea, Zea maysSpecies of your interest not listed? Contact us
Protein or membrane sample should be treated at 70 C for 10 min before loading on the gel.Antibody has a reduced reactivity to monocots in western blot.
Application Details:
1 : 2000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
73,5 | 80 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Narusaka et al (2016). Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1. Sci Rep. 2016 Jan 11;6:18702. doi: 10.1038/srep18702.
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Protein or membrane sample should be treated at 70 C for 10 min before loading on the gel, This antibody has so far not worked in IP
Application Details:
1 : 8000 (ELISA), 1 : 600 (IF), 1 : 2000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
73,5 | 80 kDa
Not reactive in:
Ostreococcus tauri
Selected references:
Gu et al. (2021) A Lipid Bodies-Associated Galactosyl Hydrolase Is Involved in Triacylglycerol Biosynthesis and Galactolipid Turnover in the Unicellular Green Alga Chlamydomonas reinhardtiiDittmer, Kleine, & Schwenkert. (2021) The TPR- and J-domain-containing proteins DJC31 and DJC62 are involved in abiotic stress responses in Arabidopsis thaliana. J Cell Sci. 2021 Oct 1;134(19):jcs259032. doi: 10.1242/jcs.259032. Epub 2021 Oct 12. PMID: 34515300.Shteinberg et al. (2021) Tomato Yellow Leaf Curl Virus (TYLCV) Promotes Plant Tolerance to Drought. Cells. 2021 Oct 25;10(11):2875. doi: 10.3390/cells10112875. PMID: 34831098; PMCID: PMC8616339.Mishra et al. (2021) Interplay between abiotic (drought) and biotic (virus) stresses in tomato plants. Mol Plant Pathol. 2021 Dec 30. doi: 10.1111/mpp.13172. Epub ahead of print. PMID: 34970822.Yang et al. (2020). PROTEIN PHOSPHATASE 95 Regulates Phosphate Homeostasis by Affecting Phosphate Transporter Trafficking in Rice. Plant Cell. 2020 Jan 9. pii: tpc.00685.2019. doi: 10.1105/tpc.19.00685.
Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
BirA (mutated/TurboID)
Expected Species:
mini TurboID
Immunogen:
Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. This tag has MW of 35 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
BirA (mutated/TurboID)
Immunogen:
Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. This tag has MW of 35 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
BirA (mutated/TurboID)
Immunogen:
Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages, This is a suitable tool for proximity labelling experiments as described in Mair et al, (2019), Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID, Elife , 2019 Sep 19;8:e47864, doi: 10,7554/eLife,47864, This tag has MW of 35 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
BirA (mutated/TurboID)
Immunogen:
Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages, This is a suitable tool for proximity labelling experiments as described in Mair et al, (2019), Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID, Elife , 2019 Sep 19;8:e47864, doi: 10,7554/eLife,47864, This tag has MW of 35 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
BirA (mutated/TurboID)
Immunogen:
Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages, This is a suitable tool for proximity labelling experiments as described in Mair et al, (2019), Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID, Elife , 2019 Sep 19;8:e47864, doi: 10,7554/eLife,47864, This tag has MW of 35 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
BirA (mutated/TurboID)
Immunogen:
Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. This tag has MW of 35 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
BirA (mutated/TurboID)
Immunogen:
Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
Black-Gold II is a novel haloaurophosphate complex which localises myelin within the central nervous system. The Black Gold II Ready-to-Dilute (RTD) Staining Kit allows you to localise myelin, both individual fibres and tracts, along with the option of co-localising cell bodies via the Toluidine Blue counter stain. Black Gold II labelled myelinated fibres appear nearly black while the Toluidine Blue O labelled cellular Nissl bodies are blue under bright field illumination. Black Gold II can demonstrate and characterise specific myelin changes associated with exposure to diverse neurotoxicants including kainic acid, domoic acid, 3-nitropropionic acid, Fluoro-Gold and isoniazid. Black Gold II can also be combined with other histochemical markers including Nissl stains, retrogradely transported fluorescent tracers and fluorescent markers of neuronal degeneration. The advantages associated with the Black-Gold II include high resolution, high contrast, short histochemical processing time, versatility and consistent reproducibility.
Product Type:
Staining Reagent
Format:
The reagents in the Black Gold kit (10X) are all supplied in a liquid format and are ready-to-dilute.
Species Reactivity:
Mammals
Applications:
IHC-Frozen,IHC-non-Paraffin-embedded
Application Details:
Black Gold II is a high resolution myelin stain with amyloid plaque counter stain. Its use is tailored to studies using formalin or paraformaldehyde fixed, non-paraffin embedded, non-solvent processed brain tissue. It can be used with both thick and thin sections. For thick sections, gelatin coated slides or slides specially designed to bind tissues sections should be use to avoid section loss. Free-floating sections can be used as well but sections are easier to handle and transfer when mounted on slides. A suggested method for thick sections is provided as a guide: Either frozen or vibratome sections are cut at a thickness of 20-50 ?m and collected in 0.1 M neutral phosphate buffer. The sections are then typically mounted on 1% gel-coated slides and then air dried on a slide warmer (at 50°C) for at least an hour until throughly dried and adhered to the slide. The sections can be stained loose, although the sections are easier to handle when mounted on slides. The mounted sections were rehydrated in distilled water for 2 minutes before being processed in the staining solutions.
Alternative Names:
BlackGold, Black and Gold
Biosensis Brand:
Biosensis® RTD
Detection Method:
Colorimetric
Shelf Life:
Unopened kit 6 months at 2-8ºC protected from light. See Storage instructions for working solutions recommendations.
Use:
For research use only.
Kit Components:
Black-Gold II (Dilute 1:10 prior to use) - 10 mL Sodium Thiosulfate, fixative (Dilute 1:10 prior to use) - 10 mL Toluidine Blue O (Dilute 1:10 prior to use) - 10 mL Acetic Acid (Dilute 1:10 prior to use) - 10 mL
Product references:
Lee J et al. (2022) PRMT1 is required for the generation of MHC-associated microglia and remyelination in the central nervous system. Life Sci Alliance. [Epub ahead of print] Germundson DL & Nagamoto-Combs K. (2022) Potential Role of Intracranial Mast Cells in Neuroinflammation and Neuropathology Associated with Food Allergy. Cells. 11(4):738. Kihara Y et al. (2021) Ponesimod inhibits astrocyte-mediated neuroinflammation and protects against cingulum demyelination via S1P1-selective modulation. FASEB J. 36(2):e22132. Toomey LM et al. (2021) Cuprizone feed formulation influences the extent of demyelinating disease pathology. Sci Rep. 11(1):22594. Del Fiacco M et al. (2018) TRPV1-Like Immunoreactivity in the Human Locus K, a Distinct Subregion of the Cuneate Nucleus. Cells. 2018 Jul 8;7(7). pii: E72. Ying YL et al. (2014) Adult neural precursor cells from the subventricular zone contribute significantly to oligodendrocyte regeneration and remyelination. J Neurosci. 2014 Oct 15;34(42):14128-46
Specificity:
Black-Gold II is a novel haloaurophosphate complex which localises myelin within the central nervous system.
Storage:
The kit can be transported at room temperature. Once received, the kit canbe stored for up to 12 months at 2-8°C protected from light. Diluted solutionscan be stored up to one month at 2-8°C protected from light.
Human blood group A antigen belongs to a group of carbohydrate determinats carried on both glycolipids and glycoproteins; it is detected on erythrocytes and certain epithelial cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.
Applications:
IHC,AGG
Additional Info:
The antibody HE-193 recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1 and 2, A antigens with chain types 3, 4, 5, 6) and Forssman antigen.
Human blood group A antigen belongs to a group of carbohydrate determinats carried on both glycolipids and glycoproteins; it is detected on erythrocytes and certain epithelial cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.
Applications:
AGG
Additional Info:
The antibody HE-195 recognizes human blood group A including weak variants A3, AX, A3B, AXB. The specifity of antibody HE-195 was confirmed by comparison of specifity and reactivity to standard reagent using >5.000 samples of blood.
The antibody HE-24 distinguishes blood group A<sub>1</sub>B from A<sub>2</sub>B. The specifity of the antibody HE-24 was verified on >1000 samples of blood.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Glycoprotein fraction isolated from saliva of secretors with blood group A.
Applications:
AGG
Additional Info:
The antibody HE-24 distinguishes blood group A1B from A2B. The specifity of the antibody HE-24 was verified on >1000 samples of blood.
The antibody HE-10 agglutinates erythrocytes of group A but does not agglutinate erythrocytes of group B and 0. Study with specific oligosaccharides showed that the antibody HE-10 reacts with A and H antigens with chain types 3 and 4 and it does not react with A disaccharide, A trisaccharide, A type 1, A type 2, ALe<sup>b</sup>.<br>The antibody HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.
Applications:
IHC
Additional Info:
The mouse monoclonal antibody HE-10 agglutinates erythrocytes of group A, and is excellent as a tumour marker in patients of blood group B and 0. It does not agglutinate erythrocytes of group B and 0. Study with specific oligosaccharides showed that the antibody HE-10 reacts with A and H antigens with chain types 3 and 4 and it does not react with A disaccharide, A trisaccharide, A type 1, A type 2, ALeb. The antibody HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues.
Clone number:
HE-10
Antibody Isotype:
IgM
Application Details:
Immunohistochemistry (paraffin sections): The antibody HE-10 is excellent as a tumour marker in patients of blood group B and 0. Flow cytometry: Recommended dilution: 1-3 : 100.
The antibody HEB-29 reacts with human blood group B. The specifity of the antibody HEB-29 was confirmed by comparison of specifity and reactivity to standard reagent using >5.000 samples of blood.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze.
Immunogen:
Mixture of erythrocytes of group B and glycoprotein fraction isolated from saliva of secretors with blood group B.
Applications:
AGG,IHC
Additional Info:
The antibody HEB-29 reacts with human blood group B. The specifity of the antibody HEB-29 was confirmed by comparison of specifity and reactivity to standard reagent using >5 000 samples of blood.
The Blook gel illumination system for the visualisation of DNA gels stained with ethidium bromide or alternatives such as NovelJuice (cat no LD001). This CE marked Blue Light LED system is more reliable than UV lamp transilluminators as the LEDs have a life time of 30,000 hours of continual use. The unit also includes an Amber filter to allow visualisation of the gel whilst protecting the eyes. The Blook has no external powerpack and takes up a minimal amount of bench space. Plus it is so light it can be stored on a standard lab shelf easily when not in use.
[SPECIFICATIONS] Dimensions (mm): 210D X 210W X 30H >> Viewing surface (mm): 120 X 70 >> Wavelength (nm): 470 >> Amber filter: amber filter shield with metal frame >> LED arrangements: matrix for two-side illumination >> LED lifetime:50,000 hours >> Power: No external power supply required >> Compatible with mini gel size (mm): 110 X 60 & 55 X 60 >> Weight (kg): 2.3 >>
Fluorescent proteins, like BFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at 2-8 C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
BFP
Immunogen:
Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
Immunoglobulin Protein A purified in a 10 mM ammonium bicarbonate buffer, with 2 mg of BSA.
Reconstitution:
Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%). Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 •Additional sodium azide ( up to 0.05%) is recommended for long term storage. •For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 μl buffer.
BOB-1 is a B-cell-specific coactivator whose expression majorly restricted to mature B-cells, and typically in germinal center B-cells. It is co-activator for Oct-1 and Oct-2 transcription factors. BOB-1 is used for B-lineage determination of CD20- plasmablastic or diffuse large B-cell lymphoma (DLBCL). BOB-1 was also presented to be useful marker when combined together with CD79a and Cyclin E for discriminating classical Hodgkins lymphoma from primary mediastinal large B-cell lymphoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC060
GMDN Code:
62794
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
BOB-1 is a B-cell-specific coactivator whose expression majorly restricted to mature B-cells, and typically in germinal center B-cells. It is co-activator for Oct-1 and Oct-2 transcription factors. BOB-1 is used for B-lineage determination of CD20- plasmablastic or diffuse large B-cell lymphoma (DLBCL). BOB-1 was also presented to be useful marker when combined together with CD79a and Cyclin E for discriminating classical Hodgkins lymphoma from primary mediastinal large B-cell lymphoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC060
GMDN Code:
62794
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
BOB-1 is a B-cell-specific coactivator whose expression majorly restricted to mature B-cells, and typically in germinal center B-cells. It is co-activator for Oct-1 and Oct-2 transcription factors. BOB-1 is used for B-lineage determination of CD20- plasmablastic or diffuse large B-cell lymphoma (DLBCL). BOB-1 was also presented to be useful marker when combined together with CD79a and Cyclin E for discriminating classical Hodgkins lymphoma from primary mediastinal large B-cell lymphoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC060
GMDN Code:
62794
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The Bombesin Antibody was raised to synthetic human bombesin coupled to bovine thyroglobulin with glutaraldehyde. The ImmunoStar bombesin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat dorsal horn of spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/400-1/600 in PBS/0.3% Triton X-100 - Cy3 Technique and 1/1,000-1/2,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of 1 mL of diluted antibody with 50 µg of bombesin.
Botulinum toxin is a toxin produced by the anaerobic, gram-positive, bacterium of the genus Clostridium (C. botulinum, C. butyricum, C. baratii and C. argentinense). These strains are widely distributed and can be found in soil and dust. Eight types of botulinum toxin are distinguished, named type A–H. Type A and B are capable of causing disease in humans (botulism) and have longest activity in vivo, and are also used commercially (BOTOX) and medically. Types C–G are less common; types E and F can cause disease in humans, while the other types cause disease in other animals. BotA is cleaved into two chains: heavy and light.Alternative name: Bontoxilysin-A
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Botulinum toxin A heavy chain
Immunogen:
Purified Botulinum Neurotoxin Type A (Heavy Chain Binding Domain)
For direct ELISA coating with 2 g of Botulinium Toxin A is recommended combined with primary antibody dilution of 1: 10 000.For Western blot 0.5 g of non reduced holotoxin or heavy chains is recommended together with 1: 2000 dilution of a primary antibody.
Botulinum toxin is a toxin produced by the anaerobic, gram-positive, bacterium of the genus Clostridium (C. botulinum, C. butyricum, C. baratii and C. argentinense). These strains are widely distributed and can be found in soil and dust. Eight types of botulinum toxin are distinguished, named type A–H. Type A and B are capable of causing disease in humans (botulism) and have longest activity in vivo, and are also used commercially (BOTOX) and medically. Types C–G are less common; types E and F can cause disease in humans, while the other types cause disease in other animals. BotA is cleaved into two chains: heavy and light. Alternative name: Bontoxilysin-A
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Lyophilized antibody can be stored at -20 °C for up to 3 years. Re-constituted antibody can be stored at 4°Cfor several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Botulinum toxin A heavy chain
Immunogen:
Recombinat C-terminal heavy chain of BoNT/A1, residues 876–1296, which was cloned into a pET28a His-tag vector (Novagen) and overexpressed in E.coli. UniProt: https://www.uniprot.org/uniprot/P10845
Botulinum toxin is a toxin produced by the anaerobic, gram-positive, bacterium of the genus Clostridium (C. botulinum, C. butyricum, C. baratii and C. argentinense). These strains are widely distributed and can be found in soil and dust. Eight types of botulinum toxin are distinguished, named type A–H. Type A and B are capable of causing disease in humans (botulism) and have longest activity in vivo, and are also used commercially (BOTOX) and medically. Types C–G are less common; types E and F can cause disease in humans, while the other types cause disease in other animals. BotB is cleaved into two chains: heavy and light.Alternative name: Bontoxilysin-B
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Botulinum Neurotoxin B fromClostridium botulinum
Immunogen:
Highly purified Botulinum Neurotoxin Type B (Clostridium botulinum)
Botulinum toxin is a toxin produced by the anaerobic, gram-positive, bacterium of the genus Clostridium (C. botulinum, C. butyricum, C. baratii and C. argentinense). These strains are widely distributed and can be found in soil and dust. Eight types of botulinum toxin are distinguished, named type A–H. Type A and B are capable of causing disease in humans (botulism) and have longest activity in vivo, and are also used commercially (BOTOX) and medically. Types C–G are less common; types E and F can cause disease in humans, while the other types cause disease in other animals. BotB is cleaved into two chains: heavy and light.Alternative name: Bontoxilysin-B
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Botulinum Neurotoxin B from Clostridium botulinum
Immunogen:
Highly purified Botulinum Neurotoxin Type B (Clostridium botulinum)
Botulinum toxin is a toxin produced by the anaerobic, gram-positive, bacterium of the genus Clostridium (C. botulinum, C. butyricum, C. baratii and C. argentinense). These strains are widely distributed and can be found in soil and dust. Eight types of botulinum toxin are distinguished, named type A–H. Type A and B are capable of causing disease in humans (botulism) and have longest activity in vivo, and are also used commercially (BOTOX) and medically. Types C–G are less common; types E and F can cause disease in humans, while the other types cause disease in other animals. BotB is cleaved into two chains: heavy and light. Alternative name: Bontoxilysin-B
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Lyophilized antibody can be stored at -20 °C for up to 3 years. Re-constituted antibody can be stored at 4°Cfor several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Botulinum toxin is a toxin produced by the anaerobic, gram-positive, bacterium of the genus Clostridium (C. botulinum, C. butyricum, C. baratii and C. argentinense). These strains are widely distributed and can be found in soil and dust. Eight types of botulinum toxin are distinguished, named type A–H. Type A and B are capable of causing disease in humans (botulism) and have longest activity in vivo, and are also used commercially (BOTOX) and medically. Types C–G are less common; types E and F can cause disease in humans, while the other types cause disease in other animals. Type E is a cause of botulism in humans. BotE is cleaved into two chains: heavy and light. Alternative names: Bontoxilysin-E, BoNT, BotE,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Antibody should be stored at -20 °C.Aliquote to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Clostridium botulinum
Immunogen:
Recombinant Botulinum Neurotoxin Type E Light Chain (Clostridium botulinum).
Botulinum toxin is a toxin produced by the anaerobic, gram-positive, bacterium of the genus Clostridium (C. botulinum, C. butyricum, C. baratii and C. argentinense). These strains are widely distributed and can be found in soil and dust. Eight types of botulinum toxin are distinguished, named type A–H. Type A and B are capable of causing disease in humans (botulism) and have longest activity in vivo, and are also used commercially (BOTOX) and medically. Types C–G are less common; types E and F can cause disease in humans, while the other types cause disease in other animals. Type E is a cause of botulism in humans. BotE is cleaved into two chains: heavy and light. Alternative names: Bontoxilysin-E, BoNT, BotE,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Antibody should be stored at -20 °C.Aliquote to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Clostridium botulinum
Immunogen:
Recombinant Botulinum Neurotoxin Type E Light Chain (Clostridium botulinum).
Botulinum toxin is a toxin produced by the anaerobic, gram-positive, bacterium of the genus Clostridium (C. botulinum, C. butyricum, C. baratii and C. argentinense). These strains are widely distributed and can be found in soil and dust. Eight types of botulinum toxin are distinguished, named type A–H. Type A and B are capable of causing disease in humans (botulism) and have longest activity in vivo, and are also used commercially (BOTOX) and medically. Types C–G are less common; types E and F can cause disease in humans, while the other types cause disease in other animals. Type E is a cause of botulism in humans. BotE is cleaved into two chains: heavy and light. Alternative names: Bontoxilysin-E, BoNT, BotE,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Antibody should be stored at -20 °C.Aliquote to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Clostridium botulinum
Immunogen:
Recombinant Botulinum Neurotoxin Type E Light Chain (Clostridium botulinum).
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Country Of Origin:
Normal Bovine Serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C, DO NOT FREEZE
Shelf Life:
1 year from date of receipt. Prepare working dilution prior to use and then discard.
Country Of Origin:
US Origin
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Bovine IgG fraction contains total bovine IgG from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Bovine immunoglobulin fraction is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. and 0.005 % sodium azide is added as preservative.Concentration is 4.5 mg/ml.
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Country Of Origin:
Normal Bovine Serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Country Of Origin:
Normal Bovine Serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Purified bovine IgG contains Protein A purified bovine IgG from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Bovine IgG is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.005 % sodium azide is added as preservative.Concentration is 4.5 mg/ml.
In phycobilisomes, the light-harvesting antennae of red algae, b-phycoerythrin consists of alpha and beta chains that contain phycoerythrobilin chromophores (an open-chain tetrapyrolle, red colored). It is found in chloroplasts of many red algae, and is closely related to the PEs of cyanobacteria and cryptomonads.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Porphyridium cruentum
Expected Species:
Algae (red), Cyanobacteria, Cryptomonads Species of your interest not listed? Contact us
Immunogen:
native purified b-phycoerythrin of Porphyridium cruentum (protein with attached phycobilisomes)
B-PE is a phycobiliprotein which is a building block of phycobilisomes, the light-harvesting complex, in red algae and some cyanobacteria. Phycobilisomes are attached to the stromal side of the thylakoid membrane. B-phycoerythrin has both phycoerythrobilin (on alpha and beta subunits) and phycourobilin (on the gamma subunits) chromophores.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Porphyridium cruentum
Expected Species:
Algae (red), Cyanobacteria, Cryptomonads Species of your interest not listed? Contact us
Immunogen:
native purified B-phycoerythrin of Porphyridium cruentum with attached chromophores
Serine/Threonine-Protein Kinase B-Raf (BRAF) is a cytoplasmic serine-threonine kinase of the RAF family, which mediates downstream cellular responses to growth signals through the mitogen-activated protein kinase (MAPK) signaling pathway. Oncogenic mutations in the BRAF gene, 80% of which are a single V600E substitution within the kinase domain, constitutively activate the MAPK signaling pathway and result in increased cell proliferation and apoptosis resistance. The V600E mutation is observed in colorectal cancer, non-Hodgkin's lymphoma, papillary thyroid carcinoma, malignant melanoma, non-small-cell lung carcinoma, and lung adenocarcinoma. BRAF V600E is therefore an important immunohistochemical marker for tumour diagnosis and prognosis.
Serine/Threonine-Protein Kinase B-Raf (BRAF) is a cytoplasmic serine-threonine kinase of the RAF family, which mediates downstream cellular responses to growth signals through the mitogen-activated protein kinase (MAPK) signaling pathway. Oncogenic mutations in the BRAF gene, 80% of which are a single V600E substitution within the kinase domain, constitutively activate the MAPK signaling pathway and result in increased cell proliferation and apoptosis resistance. The V600E mutation is observed in colorectal cancer, non-Hodgkin's lymphoma, papillary thyroid carcinoma, malignant melanoma, non-small-cell lung carcinoma, and lung adenocarcinoma. BRAF V600E is therefore an important immunohistochemical marker for tumour diagnosis and prognosis.
Serine/Threonine-Protein Kinase B-Raf (BRAF) is a cytoplasmic serine-threonine kinase of the RAF family, which mediates downstream cellular responses to growth signals through the mitogen-activated protein kinase (MAPK) signaling pathway. Oncogenic mutations in the BRAF gene, 80% of which are a single V600E substitution within the kinase domain, constitutively activate the MAPK signaling pathway and result in increased cell proliferation and apoptosis resistance. The V600E mutation is observed in colorectal cancer, non-Hodgkin's lymphoma, papillary thyroid carcinoma, malignant melanoma, non-small-cell lung carcinoma, and lung adenocarcinoma. BRAF V600E is therefore an important immunohistochemical marker for tumour diagnosis and prognosis.
Serine/Threonine-Protein Kinase B-Raf (BRAF) is a cytoplasmic serine-threonine kinase of the RAF family, which mediates downstream cellular responses to growth signals through the mitogen-activated protein kinase (MAPK) signaling pathway. Oncogenic mutations in the BRAF gene, 80% of which are a single V600E substitution within the kinase domain, constitutively activate the MAPK signaling pathway and result in increased cell proliferation and apoptosis resistance. The V600E mutation is observed in colorectal cancer, non-Hodgkin's lymphoma, papillary thyroid carcinoma, malignant melanoma, non-small-cell lung carcinoma, and lung adenocarcinoma. BRAF V600E is therefore an important immunohistochemical marker for tumour diagnosis and prognosis.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse
Immunogen:
Mixture of two human BDNF peptides (73-87 and 194-208 aa). Both peptides are highly conserved in human and mouse.
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA. Suggested dilution at 1:500 to 1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human mature BDNF expressed in E.coli
Applications:
ELISA,ICC,WB
Clone number:
3C11
Antibody Isotype:
IgG
Application Details:
ELISA (50-100 ng/mL), WB (0.2 to 2 µg/mL), IF (2 to 20 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Detects human, mouse, rat, guinea pig BDNF. Expected to detect BDNF from other species due to sequence homology.
Storage:
Store lyophilized antibody at -20°C to -80°C protected from moisture. After reconstitution divide antibody into useful aliquots and keep aliquots at -20°C to -80°C for a higher stability. Working aliquots can be kept at 2-8°C for up to 1 month. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 0.1% trehalose, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human mature BDNF expressed in E.coli
Applications:
ELISA,WB
Clone number:
4C8
Antibody Isotype:
IgG
Application Details:
<b>ELISA:</b> 50-100 ng/mL <br><br><b>Western Blotting (denaturing and reducing):</b> 0.2 to 2 µg/mL. M-1744-100 antibody detects 14 kDa mature BDNF monomer in human serum human and acid treated human and rodent whole tissue homogenates. In simple Tris-homogenate human brain lysates, M-1744-100 detects mature BDNF at 14kDa as well as an uncharacterized band at ~50kDa. No proBDNF band is detected in our samples, although M-1744-100 will detect proBDNF in purified form on blots. Additionally, M-1744-100 detects an additional band 18 kDa BDNF isoform human serum. For cell lysates, some caution is advised as in our hands M-1744-100 fails to detect mature BDNF on SH-SY5Y or C6 cell lysates but does detect mature BDNF in neuronal cell culture lysates. The reason for these differences has not been characterized. For detection of mature BDNF in cell lysates such as SH-SY5Y we recommend affinity-purified rabbit polyclonal antibody to rhBDNF (R-1707-100), or rabbit polyclonal antibody to BDNF peptide 1-10 (R-083-100, whole serum; R-066-500, IgG).<br><br><b>Immunofluorescence:</b> 1 to 4 µg/mL, 4% formaldehyde fixation and permeabilization. Staining can be weak until optimized.<br><br>M-1744-100 is not recommended for Flow Cytometry at this time.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Le Blanc J et al. (2020) Platelets Selectively Regulate the Release of BDNF, But Not That of Its Precursor Protein, proBDNF. Front Immunol. 11:575607 Application: Human, WB.
Specificity:
Detects human, mouse, rat, guinea pig BDNF. Expected to detect BDNF from other species due to sequence homology.
Storage:
Store lyophilized antibody at -20°C to -80°C protected from moisture. After reconstitution divide antibody into useful aliquots and keep aliquots at -20°C to -80°C for a higher stability. Working aliquots can be kept at 2-8°C for up to 1 month. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 0.1% trehalose, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human mature BDNF expressed in E.coli
Applications:
ELISA,WB
Clone number:
4C8
Antibody Isotype:
IgG
Application Details:
<b>ELISA:</b> 50-100 ng/mL <br><br><b>Western Blotting (denaturing and reducing):</b> 0.2 to 2 µg/mL. M-1744-100 antibody detects 14 kDa mature BDNF monomer in human serum human and acid treated human and rodent whole tissue homogenates. In simple Tris-homogenate human brain lysates, M-1744-100 detects mature BDNF at 14kDa as well as an uncharacterized band at ~50kDa. No proBDNF band is detected in our samples, although M-1744-100 will detect proBDNF in purified form on blots. Additionally, M-1744-100 detects an additional band 18 kDa BDNF isoform human serum. For cell lysates, some caution is advised as in our hands M-1744-100 fails to detect mature BDNF on SH-SY5Y or C6 cell lysates but does detect mature BDNF in neuronal cell culture lysates. The reason for these differences has not been characterized. For detection of mature BDNF in cell lysates such as SH-SY5Y we recommend affinity-purified rabbit polyclonal antibody to rhBDNF (R-1707-100), or rabbit polyclonal antibody to BDNF peptide 1-10 (R-083-100, whole serum; R-066-500, IgG).<br><br><b>Immunofluorescence:</b> 1 to 4 µg/mL, 4% formaldehyde fixation and permeabilization. Staining can be weak until optimized.<br><br>M-1744-100 is not recommended for Flow Cytometry at this time.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Le Blanc J et al. (2020) Platelets Selectively Regulate the Release of BDNF, But Not That of Its Precursor Protein, proBDNF. Front Immunol. 11:575607 Application: Human, WB.
Specificity:
Detects human, mouse, rat, guinea pig BDNF. Expected to detect BDNF from other species due to sequence homology.
Storage:
Store lyophilized antibody at -20°C to -80°C protected from moisture. After reconstitution divide antibody into useful aliquots and keep aliquots at -20°C to -80°C for a higher stability. Working aliquots can be kept at 2-8°C for up to 1 month. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human mature BDNF expressed in E.coli
Applications:
ELISA,ICC,WB
Clone number:
3C11
Antibody Isotype:
IgG
Application Details:
ELISA (50-100 ng/mL), WB (0.2 to 2 µg/mL), IF (2 to 20 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Detects human, mouse, rat, guinea pig BDNF. Expected to detect BDNF from other species due to sequence homology.
Storage:
Store lyophilized antibody at -20°C to -80°C protected from moisture. After reconstitution divide antibody into useful aliquots and keep aliquots at -20°C to -80°C for a higher stability. Working aliquots can be kept at 2-8°C for up to 1 month. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.05% sodium azide.
Host Animal:
Rabbit
Species Reactivity:
Human,Other Mammals (Predicted)
Immunogen:
Antibody was raised against a GST-tagged rhBDNF fusion protein and expressed in and purified from E. coli.
Applications:
ELISA
Antibody Isotype:
IgG
Application Details:
<b>Western Blotting (denaturing and reducing):</b> 0.2 to 1 µg/mL. Antibody detects 14 kDa mature BDNF monomer and 32 kDa proBDNF monomer in cell lysate and tissue homonenate. Antibody has only been tested on cell lysate and tissue homogenate of human origin. Acid-treated samples may give cleaner blots, and enhance signals for BDNF. R-1707-100 is not recommended for human serum samples. For human serum analysis, we recommend mouse monoclonal antibody to rhBDNF (M-1744-50/100), or rabbit polyclonal antibody to BDNF peptide 1-10 (R-083-100, whole serum; R-066-500, IgG).<br><br><b>Flow Cytometry:</b> ~2 µg per 10^6 cells, methanol fixation. Note: R-1707-100 cannot be used to distinguish the flow cytometry signal originating from mature BDNF versus proBDNF.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Brain-derived neurotrophic factor; Abrineurin
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human, rat and mouse BDNF. Expected to detect BDNF from other species due to sequence homology. No cross-reactivity with other neurotrophins.
Storage:
Store lyophilized antibody at 2-8°C protected from moisture. After reconstitution divide antibody into useful aliquots and keep aliquots at -20°C to -80°C for a higher stability. Working aliquots can be kept at 2-8°C for up to 1 month. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst primates.
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
<b>Western Blotting:</b> This antibody detects multiple BDNF isoforms (14 kDa mature BDNF, 18 kDa isoform, 28 kDa BDNF dimer/truncated BDNF, 32 kDa proBDNF monomer) depending on sample application (human serum, cell lysate, tissue homogenate). Antibody also detects BDNF under non-reducing conditions (McLean NA, 2014).<br><br><b>ELISA:</b> Detection only, 1:1000-1:5000 recommended.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
McLean NA, Popescu BF, Gordon T, Zochodne DW, Verge VM. (2014) "Delayed nerve stimulation promotes axon-protective neurofilament phosphorylation, accelerates immune cell clearance and enhances remyelination in vivo in focally demyelinated nerves." PLoS One. 2014 Oct 13;9(10):e110174 Application: WB , non-reducing, Species: Rat Cysneiros R.M. et al (2010) Qualitative analysis of hippocampal plastic changes in rats with epilepsy supplemented with oral omega-3 fatty acids Epilepsy Behav. 2010 Jan;17(1):33-8. Ooe N. et al (2009) Dynamic regulation of bHLH-PAS-type transcription factor NXF gene expression and neurotrophin dependent induction of the transcriptional control activity Biochem Biophys Res Commun. 2009 Jan 23;378(4):761-5.
Specificity:
Less than 0.1% cross reactivity with mouse NGF, recombinant human NT3 and NT4/5 has been recorded by dot blot analysis. This antiserum is known to recognise rat, mouse and human BDNF, and is expected to react with BDNF from other species due to amino acid sequence homology.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human BDNF
Applications:
IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA (1 site), Western Blot, inhibition of biological activity in vitro/in vivo. Recommended to be used at a dilution of 1:1000 for immunohistochemistry, ELISA and Western blot. 1:10 to 1:50 for inhibition of biological activity in vitro. Use neat for in vivo studies at 5-10 uL/g body weight. This antiserum stains cell bodies and some nerve terminals in the dorsal horn of the rat spinal cord, however, does not stain finest nerve terminals. <br><br>Western Blotting: Antibody does detect BDNF forms in tissue lysates but there are multiple bands present, many of which are uncharacterized. The antibody detects 14 kDa (mature BDNF), 32 kDa (proBDNF) and a 18 kDa BDNF isoform (see blot examples). In cell lysates, only 18 kDa and 32 kDa BDNF are detected. The reason for these differences has not been characterized. Alternative antibodies for Western Blotting are: R-017-500 (IgG-purified form of R-088-100 for tissue homogenate analysis); R-1707-100 (cell lysates and tissue homogenates), R-083-100/R-066-500 (cell lysates, tissue homogenates and human serum); M-1744-50/100 (human serum and tissue homogenates).<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
L.Y. Chen et al (2010) Learning induces neurotrophin signaling at hippocampal synapses Proc Natl Acad Sci USA. Apr 13;107(15):7030-5 Soderquist R.G. et al (2009) PEGylation of brain-derived neurotrophic factor for preserved biological activity and enhanced spinal cord distribution J Biomed Mater Res A. 2009 Dec;91(3):719-29. Tang S. et al (2010) Immunolocalization of pro- and mature-brain derived neurotrophic factor (BDNF) and receptor TrkB in the human brainstem and hippocampus. Brain Res. Oct 1;1354:1-14. Sadri-Vakili G. et al (2010) Cocaine-induced chromatin remodeling increases brain-derived neurotrophic factor transcription in the rat medial prefrontal cortex, which alters the reinforcing efficacy of cocaine. J Neurosci. 2010 Sep 1;30(35):11735-44. Maldonado M.A. et al (2008) Motor skill training, but not voluntary exercise, improves skilled reaching after unilateral ischemic lesions of the sensorimotor cortex in rats. Neurorehabil Neural Repair. 2008 May-Jun;22(3):250-61. Nakajima H. et al (2007) Rescue of rat anterior horn neurons after spinal cord injury by retrograde transfection of adenovirus vector carrying brain-derived neurotrophic factor gene. J Neurotrauma. 2007 Apr;24(4):703-12. Zhang H.T. et al (2007) Immunohistochemical distribution of NGF, BDNF, NT-3, and NT-4 in adult rhesus monkey brains. J Histochem Cytochem. 2007 Jan;55(1):1-19. Carrasco M.A. et al (2007) Regulation of glycinergic and GABAergic synaptogenesis by brain-derived neurotrophic factor in developing spinal neurons. Neuroscience. 2007 Mar 16;145(2):484-94. Zhang H.T. et al (2008) Temporal changes in the level of neurotrophins in the spinal cord and associated precentral gyrus following spinal hemisection in adult Rhesus monkeys J Chem Neuroanat. 2008 Dec;36(3-4):138-43.
Specificity:
Less than 0.1% cross-reactivity against NGF, NT3 and NT4/5 by dot blot. Known to react with BDNF from rat, mouse and human. Expected to react with BDNF from other species due to amino acid sequence homology.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer, and Parkinson disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain.<br />FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst mammalian species.
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
<b>Western Blotting:</b> A concentration of 1-10 µg/mL is recommended for this application. In Western Blotting, this antibody detects multiple BDNF isoforms (14 kDa mature BDNF, 18 kDa isoform, 28 kDa BDNF dimer/truncated BDNF, 32 kDa proBDNF monomer) depending on sample application (human serum, cell lysate, tissue homogenate).<br><br><b>IHC:</b> Antibody works well in immunohistochemistry with the proper fixation, pretreatments and dilution. Formal fixed, paraffin embedded tissue is not recommend. Recommended fixation is Zamboni fixative or light 4% PFA fixation on fixed, frozen tissue. Recommended dilution is 1-10 µg/mL for immunohistochemistry at 4 degrees centigrade for 2-48 hours. <b>ELISA:</b> 1-10 µg/mL capture/detection.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Feron F et al (2008) Neurotrophin expression in the adult olfactory epithelium. Brain Res. 1196:13-21 Application: IHC ; Species: Rat
Specificity:
Less than 0.1% cross reactivity with mouse NGF, recombinant human NT3 and NT4/5 has been recorded by dot blot analysis. This antiserum is known to recognise rat, human and human BDNF, and is expected to react with BDNF from other species due to amino acid sequence homology.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human BDNF
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA (1 site), Western Blot, immunoblot, inhibition of biological activity in vitro/in vivo. Recommended to be used at an amount of 1-10 µg/mL for immunohistochemistry, Western blot or immunoblot, 0.1 µg/mL for ELISA and for inhibition of biological activity in vitro 1-10 µg/mL. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum stains cell bodies and some nerve terminals in the dorsal horn of the rat spinal cord, however, does not stain finest nerve terminals. Western blot: 1-10 µg/mL. Tissue homogenate is a recommended sample application for Western Blotting. The antibody detects 14 kDa (mature BDNF), 32 kDa (proBDNF) and a 18 kDa BDNF isoform, however numerous other non-characterized bands may also be present. In cell lysates, only 18 kDa and 32 kDa BDNF are detected. Alternative antibodies for Western Blotting are: R-1707-100 (cell lysates and tissue homogenates), R-083-100/R-066-500 (cell lysates, tissue homogenates and human serum); M-1744-50/100 (human serum and tissue homogenates).<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Serra, M.P. et al. (2022) Anti-Inflammatory Effect of Beta-Caryophyllene Mediated by the Involvement of TRPV1, BDNF and trkB in the Rat Cerebral Cortex after Hypoperfusion/Reperfusion Int. J. Mol. Sci. Mar; 23(7),3633 Lin J.C-Y. and Rosenthal A. (2011) Methods for treating obesity by administering a trkB antagonist US Patent US 7935342 B2 Counts S.E. and Mufson E.J. (2010) Noradrenaline activation of neurotrophic pathways protects against neuronal amyloid toxicity J Neurochem. 2010 May;113(3):649-60. Unsain N. et al (2009) Brain-derived neurotrophic factor facilitates TrkB down-regulation and neuronal injury after status epilepticus in the rat hippocampus J Neurochem. 2009 Oct;111(2):428-40 Salehi Z., Mashayekhi F. (2009) Brain-derived neurotrophic factor concentrations in the cerebrospinal fluid of patients with Parkinson's disease J Clin Neurosci. Jan;16(1):90-3.
Specificity:
Less than 0.1% cross-reactivity against NGF, recombinant NT3 and NT4 by dot blot. This antiserum is known to react with BDNF from rat, mouse and human. Expected to react with BDNF of other species due to amino acid sequence homology.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human BDNF
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, Inhibition of biological activity in vitro/in vivo. Recommended to be used at a dilution of 1:200-2000 for immunohistochemistry on Zamboni's fixed frozen tissue; not recommended for formalin fixed paraffin embedded tissues. 1:10 to 1:50 for inhibition of biological activity in vitro. Use neat for in vivo studies at 5-10 µL/g body weight. Whole serum format will caused immune responses, purified format is preferred for most in vivo work. Not recommended for western blots. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A cross reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by one site ELISA. This antiserum recognises BDNF from rat, mouse and human.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
Recombinant human BDNF
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, Inhibition of biological activity in vitro/in vivo, ELISA. Recommended to be used at an amount of 1-10 µg/mL for immunohistochemistry on Zamboni's fixed, frozen tissue. Not recommended for paraffin embedded tissues. Primary use is for biological activity in vitro and in vivo. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antibody does not react to BDNF in western blot, thus western blot is not a recommended application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Beros J. et al. (2021) Age Related Response of Neonatal Rat Retinal Ganglion Cells to Reduced TrkB Signaling in vitro and in vivo. Front Cell Dev Biol. 9:671087. Beros J. (2020) Pretreatment of ovaries with collagenase before vitrification keeps the ovarian reserve by maintaining cell-cell adhesion integrity in ovarian follicles. PhD Thesis. Hayashida K., Eisenach JC. (2010) Spinal alpha2-adrenoceptor-mediated analgesia in neuropathic pain reflects brain-derived nerve growth factor and changes in spinal cholinergic neuronal function. Anesthesiology. 2010 Aug;113(2):406-12. Geremia NM. et al. (2010) Endogenous BDNF regulates induction of intrinsic neuronal growth programs in injured sensory neurons. Exp Neurol. 2010 May;223(1):128-42.
Specificity:
A cross reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by one site ELISA. Known to react with BDNF from rat and human.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
The Biosensis CE Marked BDNF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the preferential quantification of mature BDNF in less than 3 hours. This kit consists of a pre-coated mouse monoclonal anti-BDNF capture antibody, a biotinylated anti-BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of BDNF present in samples and protein standards. This BDNF ELISA kit employs a recombinant human BDNF standard approved by the World Health Organization (WHO, www.nibsc.org ). This kit is suitable to measure mature BDNF in human serum and citrate-treated plasma samples only. The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein and therefore recognize the mature as well as the pro-form of BDNF. However, cross-reactivity to the full-length proBDNF protein is low. This CE Marked BDNF Rapid ELISA [Cat. No. BEK-2211-CE] Kit is approved for in-vitro diagnostic (IVD) applications in the European Economic Area (EEA). It has been developed by Biosensis and is manufactured by Calbiotech Inc. ( www.calbiotech.com ) for Biosensis. BEK-2211-CE is not approved for in-vitro diagnostic (IVD) applications in the United States. For research on human blood, customers MUST order the catalog number BEK-2211 . This research-use-only ELISA kit can be used for human and animal research purposes worldwide, and has been validated for a wider range of sample types and species.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant human BDNF with an N-terminal methionine residue, made in E. coli (WHO reference reagent)
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, mature (BDNF, mature) in Serum, Plasma (Citrate). BEK-2211-CE is expressly designed and tested only for use on human blood and plasma samples. Any other use is deemed "off label use" and thus the performance characteristics of the assay will have to be determined by the end user, and such results are not supported by Biosensis or CalBioTech at this time. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
See BEK-2211-1P-CE protocol insert for specific expiration dating of the kit and its components.
Use:
Approved for in-vitro diagnostic (IVD) applications in the European Economic Area (EEA). It has been developed by Biosensis and is manufactured by Calbiotech Inc. (www.calbiotech.com) for Biosensis.
This kit is not approved for in-vitro diagnostic (IVD) applications in the United States. For research on human blood, customers MUST order the catalog number BEK-2211.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Reed JL et al. (2021) The effects of high-intensity interval training, Nordic walking and moderate-to-vigorous intensity continuous training on functional capacity, depression and quality of life in patients with coronary artery disease enrolled in cardiac rehabilitation: A randomized controlled trial (CRX study). Prog Cardiovasc Dis. [Epub ahead of print]. Application: Human blood. Valkenborghs SR et al. (2019) Aerobic exercise and consecutive task-specific training (AExaCTT) for upper limb recovery after stroke: A randomized controlled pilot study. Physiother Res Int. [Epub ahead of print]. Application: Human serum.
Specificity:
Human BDNF when used as directed.
Storage:
Store at 2-8°C
Range:
7.8 pg/mL - 500 pg/mL
Sample Type:
Plasma (Citrate),Serum
Sensitivity:
Typical limit of detection (LOD) for BDNF is < 3 pg/mL determined by calculating the mean + 2x standard deviation of mean of blank (n=20).
The Biosensis CE Marked BDNF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the preferential quantification of mature BDNF in less than 3 hours. This kit consists of a pre-coated mouse monoclonal anti-BDNF capture antibody, a biotinylated anti-BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of BDNF present in samples and protein standards. This BDNF ELISA kit employs a recombinant human BDNF standard approved by the World Health Organization (WHO, www.nibsc.org ). This kit is suitable to measure mature BDNF in human serum and citrate-treated plasma samples only. The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein and therefore recognize the mature as well as the pro-form of BDNF. However, cross-reactivity to the full-length proBDNF protein is low. This CE Marked BDNF Rapid ELISA [Cat. No. BEK-2211-CE] Kit is approved for in-vitro diagnostic (IVD) applications in the European Economic Area (EEA). It has been developed by Biosensis and is manufactured by Calbiotech Inc. ( www.calbiotech.com ) for Biosensis. BEK-2211-CE is not approved for in-vitro diagnostic (IVD) applications in the United States. For research on human blood, customers MUST order the catalog number BEK-2211 . This research-use-only ELISA kit can be used for human and animal research purposes worldwide, and has been validated for a wider range of sample types and species.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human
Immunogen:
Recombinant human BDNF with an N-terminal methionine residue, made in E. coli (WHO reference reagent)
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, mature (BDNF, mature) in Serum, Plasma (Citrate). BEK-2211-CE is expressly designed and tested only for use on human blood and plasma samples. Any other use is deemed "off label use" and thus the performance characteristics of the assay will have to be determined by the end user, and such results are not supported by Biosensis or CalBioTech at this time. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
See BEK-2211-2P-CE protocol insert for specific expiration dating of the kit and its components.
Use:
Approved for in-vitro diagnostic (IVD) applications in the European Economic Area (EEA). It has been developed by Biosensis and is manufactured by Calbiotech Inc. (www.calbiotech.com) for Biosensis.
This kit is not approved for in-vitro diagnostic (IVD) applications in the United States. For research on human blood, customers MUST order the catalog number BEK-2211.
Kit Components:
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Reed JL et al. (2021) The effects of high-intensity interval training, Nordic walking and moderate-to-vigorous intensity continuous training on functional capacity, depression and quality of life in patients with coronary artery disease enrolled in cardiac rehabilitation: A randomized controlled trial (CRX study). Prog Cardiovasc Dis. [Epub ahead of print]. Application: Human blood. Valkenborghs SR et al. (2019) Aerobic exercise and consecutive task-specific training (AExaCTT) for upper limb recovery after stroke: A randomized controlled pilot study. Physiother Res Int. [Epub ahead of print]. Application: Human serum.
Specificity:
Human BDNF when used as directed.
Storage:
Store at 2-8°C
Range:
7.8 pg/mL - 500 pg/mL
Sample Type:
Plasma (Citrate),Serum
Sensitivity:
Typical limit of detection (LOD) for BDNF is < 3 pg/mL determined by calculating the mean + 2x standard deviation of mean of blank (n=20).
The Biosensis Mature BDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mature BDNF in less than 3 hours in cell culture supernatants, serum, plasma (citrate and EDTA), pig serum, cell lysates, brain extracts, human milk and Sheep CSF only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Please refer to the kit protocol for specific use instructions for each substrate application, in particular blood samples, human milk and CSF. Note that accurate quantification of BDNF in human milk requires a secretory IgA (sIgA) blocker which can be purchased separately ( BL-001-1250 ). For measurement of mature BDNF in CSF samples, please contact us at sales@biosensis.com . This ELISA kit has been tested in independent research laboratories and found to achieve highest reproducibility with intra- and inter-assay CVs as low as 1% and 5%, respectively (Polacchini et al., 2015). This ELISA kit consists of a pre-coated mouse monoclonal anti-mature BDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature BDNF present in samples and protein standards. A BDNF positive control (QC sample) is provided to assure consistent assay performance. This Mature BDNF ELISA kit employs a recombinant human mature BDNF standard approved by the World Health Organization (WHO, www.nibsc.org ). The amino acid sequence of mature BDNF is identical for human, mouse, rat and a number of other species. This kit therefore is suitable to measure mature BDNF in all these species and uses the same antibodies and antigen. Extensive validation has demonstrated that the Mature BDNF Rapid TM ELISA shows only minimal cross-reactivity with proBDNF. Please refer to our Technical Note #5 for further details on ELISA assay validation for BDNF isoform detection and quanification. This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures. For in-vitro diagnostic (IVD) applications in the European Economic Area (EEA), we refer to the CE Marked BDNF ELISA kit (BEK-2211-CE) .
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human BDNF with an N-terminal methionine residue, made in E. coli (WHO reference reagent)
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, mature (BDNF, mature) in Culture Supernatant, Serum, Plasma (Citrate), Plasma (EDTA), Cell Lysates, Tissue Homogenates, Human Milk, CSF. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Total Number of References: 99 Latest Publications (2019-2022):
Meshkat S et al. (2022) Brain-Derived Neurotrophic Factor (BDNF) as a biomarker of treatment response in patients with Treatment Resistant Depression (TRD): A systematic review & meta-analysis Psychiatry Res. 317:114857 Application: Human, serum. Cook A et al. (2022) Activation of TrkB-Akt signaling rescues deficits in a mouse model of SCA6 Sci Adv. [Epub ahead of print] Application: Mouse, brain extracts. Tsotsoros CE et al. (2022) Pilot Associations between Adverse Childhood Experiences, Executive Function, and Brain-Derived Neurotrophic Factor (BDNF) among Adults with Excess Adiposity Obesities. 2, 276-284. Application: Human, serum. Salem HA et al. (2022) Neuroprotective Effect of Morin Hydrate against Attention-Deficit/Hyperactivity Disorder (ADHD) Induced by MSG and/or Protein Malnutrition in Rat Pups: Effect on Oxidative/Monoamines/Inflammatory Balance and Apoptosis Pharmaceuticals. 15, 1012. Application: Rat, brain supernatant. Aldhshan MS & Mizuno TM. (2022) Effect of environmental enrichment on aggression and the expression of brain-derived neurotrophic factor transcript variants in group-housed male mice Behav Brain Res. [Epub ahead of print]. Application: Mouse, brain tissue homogenate. Fujino M et al. (2022) Orally Administered Plasmalogens Alleviate Negative Mood States and Enhance Mental Concentration: A Randomized, Double-Blind, Placebo-Controlled Trial Front Cell Dev Biol. 10:894734 Application: Human, plasma. Abrial E et al. (2022) Investigating Predictive Factors of Suicidal Re-attempts in Adolescents and Young Adults After a First Suicide Attempt, a Prospective Cohort Study. Study Protocol of the SURAYA Project Front. Psychiatry. [Epub ahead of print] Application: Human, plasma. Tanaka-Kanegae R et al. (2022) Sufficiently Elevated Core Body Temperature May Be Necessary to Maintain Cerebral Blood Flow Response throughout the Morning Neurosci Med. 13, 70-90 Application: Human, serum. Merlo S et al. (2022) Microglial polarization differentially affects neuronal vulnerability to the ?-amyloid protein: Modulation by melatonin Biochem Pharmacol. 202:115151 Application: Human, cell culture supernatant. Dalile B et al. (2022) Extruded Wheat Bran Consumption Increases Serum Short-Chain Fatty Acids but Does Not Modulate Psychobiological Functions in Healthy Men: A Randomized, Placebo-Controlled Trial Front Nutr. 9:896154 Application: Human, serum. Agapouda A et al. (2022) Rhodiola Rosea Extract Counteracts Stress in an Adaptogenic Response Curve Manner via Elimination of ROS and Induction of Neurite Outgrowth Oxid. Med. Cell. Longev. [Epub ahead of print] Application: Human, cell lysates. Breazeale S et al. (2022) Symptom cluster profiles following traumatic orthopaedic injuries Injury. [Epub ahead of print] Application: Human, serum. Jaehne EJ et al. (2022) Behavioral phenotyping of a rat model of the BDNF Val66Met polymorphism reveals selective impairment of fear memory Transl Psychiatry. 12(1):93 Application: Rat, acid extracted tissue lysates. Wang RY et al. (2022) The SDF1-CXCR4 Axis Is Involved in the Hyperbaric Oxygen Therapy-Mediated Neuronal Cells Migration in Transient Brain Ischemic Rats. Int J Mol Sci. 23, 1780 Application: Rat, brain tissue homogenate and serum. Hugues N et al. (2022) Time-Dependent Cortical Plasticity during Moderate-Intensity Continuous Training Versus High-Intensity Interval Training in Rats. Cereb Cortex. [Epub ahead of print] Application: Rat, cortical tissue homogenate. Cefis M et al. (2021) Endothelial cells are an important source of BDNF in rat skeletal muscle. Sci Rep. 12(1):311 Application: Rat, skeletal muscle tissue homogenate. Becker AM et al. (2021) Acute Effects of Psilocybin After Escitalopram or Placebo Pretreatment in a Randomized, Double-Blind, Placebo-Controlled, Crossover Study in Healthy Subjects. Clin Pharmacol Ther. [Epub ahead of print] Application: Human, plasma. Berbenetz N et al. (2021) The Relationship Between Brain Derived Neurotrophic Factor (BDNF) and Symptoms Following Catheter Ablation for Paroxysmal Atrial Fibrillation (AF)- NEURO-AF Study. Circulation. [Epub ahead of print] Application: Human, serum. Walsh JJ et al. (2021) Short-term ketone monoester supplementation improves cerebral blood flow and cognition in obesity: A randomized cross-over trial. J Physiol. [Epub ahead of print] Application: Human, serum, platelet-poor plasma. Boukhatem I et al. (2021) The brain-derived neurotrophic factor prompts platelet aggregation and secretion. Blood Adv. 5(18):3568-3580 Application: Human, plasma. Yi X et al. (2021) Serum mBDNF and ProBDNF Expression Levels as Diagnosis Clue for Early Stage Parkinson's Disease. Front Neurol. 12:680765 Application: Human, serum. Inoue T et al. (2021) Ipsilateral BDNF mRNA expression in the motor cortex positively correlates with motor function of the affected forelimb after intracerebral hemorrhage. Brain Res. [Epub ahead of print] Application: Rat, brain homogenate. Shoshina II et al. (2021) Visual processing and BDNF levels in first-episode schizophrenia. Psychiatry Res. [Epub ahead of print] Application: Human, serum. Cappoli N et al. (2021) Effects of remifentanil on human C20 microglial pro-inflammatory activation. Eur Rev Med Pharmacol Sci. 25(16):5268-5274 Application: Human, cell culture. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print] Application: Human, urine. Mori Y et al. (2021) Serum BDNF as a Potential Biomarker of Alzheimer's Disease: Verification Through Assessment of Serum, Cerebrospinal Fluid, and Medial Temporal Lobe Atrophy. Front Neurol. 12:653267 Application: Human, serum. Seno S et al. (2021) Effects of Selective Serotonin Reuptake Inhibitors on Depression-Like Behavior in a Laser-Induced Shock Wave Model. Front Neurol. 12:602038 Application: Mouse, hippocampal homogenates. Medeiros GC et al. (2021) Treatment of depression with ketamine does not change plasma levels of brain-derived neurotrophic factor or vascular endothelial growth factor. J Affect Disord. 280(Pt A):136-139 Application: Human, plasma. Dorandish S et al. (2021) Differences in the Relative Abundance of ProBDNF and Mature BDNF in A549 and H1299 Human Lung Cancer Cell Media. Int J Mol Sci. 22(13):7059 Application: Human, culture supernatant. Yap NY et al. (2021) Relationship between cytokines and brain-derived neurotrophic factor (BDNF) in trajectories of cancer-related cognitive impairment. Cytokine. [Epub ahead of print] Application: Human, plasma. Wang L et al. (2021) The mediating effect of brain-derived neurotrophic factor levels on childhood trauma and psychiatric symptoms in patients with first-episode schizophrenia. Aust N Z J Psychiatry. [Epub ahead of print] Application: Human. Mallik SB et al. (2021) Remedial effects of caffeine against depressive-like behaviour in mice by modulation of neuroinflammation and BDNF. Nutr Neurosci. [Epub ahead of print] Application: Mouse. Nomura S et al. (2021) Effects of a Tea Cultivar "MK5601" on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice. J Nutr Sci Vitaminol (Tokyo). 67(3):170-179 Application: Mouse, hippocampal RIPA homogenates. Caruso GI et al. (2021) SIRT1-Dependent Upregulation of BDNF in Human Microglia Challenged with A?: An Early but Transient Response Rescued by Melatonin. Biomedicines. 9(5):466 Application: Human, cell culture supernatant. Miller KM et al. (2021) Striatal Afferent BDNF Is Disrupted by Synucleinopathy and Partially Restored by STN DBS. J Neurosci. 41(9):2039-52 Application: Rat, tissue homogenates (RIPA). Vickneson K et al. (2021) Cold-induced dishabituation in rodents exposed to recurrent hypoglycaemia. Diabetologia. 64(6):1436-41 Application: Rat, blood. Li P et al. (2021) Intermediation of perceived stress between early trauma and plasma M/P ratio levels in obsessive-compulsive disorder patients. J Affect Disord. 285:105-111 Application: Human, plasma. Lai NS et al. (2021) Increased Serum Levels of Brain-Derived Neurotrophic Factor Contribute to Inflammatory Responses in Patients with Rheumatoid Arthritis. Int. J. Mol. Sci. 22(4):1841 Application: Human, serum and culture supernatants. Pan S et al. (2021) The microRNA-195 - BDNF pathway and cognitive deficits in schizophrenia patients with minimal antipsychotic medication exposure. Transl Psychiatry. 11(1):117 Application: Human, plasma. Miyamoto T et al. (2021) Effect of pedaling cadence on serum levels of brain-derived neurotrophic factor during ergometric exercise in healthy adults. Sport Sci Health. Application: Human, serum. Normann AJ (2020) The Effect of Light Therapy and Acute Aerobic Exercise on Serum Brain Derived Neurotrophic Factor in Older Adults. MSc Thesis. Application: Human, serum. Holze F et al. (2020) Acute dose-dependent effects of lysergic acid diethylamide in a double-blind placebo-controlled study in healthy subjects. Neuropsychopharmacology. [Epub ahead of print]. Application: Human, plasma. Hasler G et al. (2020) The Association Between Adolescent Residential Mobility and Adult Social Anxiety, BDNF and Amygdala-Orbitofrontal Functional Connectivity in Young Adults With Higher Education. Front. Psychiatry. Application: Human, serum. Wallace AW (2020) The Impact of Six Weeks of Intermittent Fasting, With and Without Aerobic Exercise, on Serum BDNF in Young Adult Males. MSc Thesis. Application: Human, serum. Walsh JJ et al. (2020) The Effect of Exogenous Ketone Monoester Ingestion on Plasma BDNF During an Oral Glucose Tolerance Test. Front Physiol. 11:1094. Application: Human, plasma. Meade GM et al. (2020) A Model of Negative Emotional Contagion Between Male-Female Rat Dyads: Effects of Voluntary Exercise on Stress-Induced Behavior and BDNF-TrkB Signaling. Physiol Behav. 113286 Application: Rat, serum. Hutten NRPW et al. (2020) Low Doses of LSD Acutely Increase BDNF Blood Plasma Levels in Healthy Volunteers. ACS Pharmacol. Transl. Sci. Application: Human, plasma. Barbosa AC et al. (2020) Assessment of BDNF serum levels as a diagnostic marker in children with autism spectrum disorder. Sci Rep. 10(1):17348. Application: Human, serum. Okamura M et al. (2020) Low-Level Inhibition of GABAergic Synapses Enhances Gene Expressions Crucial for Neuronal Plasticity in the Hippocampus After Ischemic Stroke. J Stroke Cerebrovasc Dis. 29(12):105316. Application: Rat, hippocampus homogenate. Payne AJ et al. (2020) The Effects of Alcohol on BDNF and CD5 Dependent Pathways. PhD Thesis. Application: Mouse, RIPA tissue homogenate. Nishiyama M et al. (2020) Homostachydrine is a Xenobiotic Substrate of OCTN1/SLC22A4 and Potentially Sensitizes Pentylenetetrazole-Induced Seizures in Mice. Neurochem Res. [Epub ahead of print]. Application: Mouse, acid-extracted hippocampal homogenate. Lorinczova HT et al. (2020) Co-Administration of Iron and a Bioavailable Curcumin Supplement Increases Serum BDNF Levels in Healthy Adults. Antioxidants (Basel). 9(8):E645. Application: Human serum. Yap NY F et al. (2020) Associations of plasma brain-derived neurotrophic factor (BDNF) and Val66Met polymorphism (rs6265) with long-term cancer-related cognitive impairment in survivors of breast cancer. Breast Cancer Res Treat. [Epub ahead of print]. Application: Human plasma. Vasilopoulou F et al. (2020) Amelioration of BPSD-Like Phenotype and Cognitive Decline in SAMP8 Mice Model Accompanied by Molecular Changes After Treatment With I 2-Imidazoline Receptor Ligand MCR5. Pharmaceutics. 12(5):E475. Application: Mouse hippocampus RIPA-homogenates. Mueller ST et al. (2020) Negative Association Between Left Prefrontal GABA Concentration and BDNF Serum Concentration in Young Adults. Heliyon. 6(5):e04025. Application: Human serum. Chen LF et al. (2020) The NMDA receptor subunit GluN3A regulates synaptic activity-induced and myocyte enhancer factor 2C (MEF2C)-dependent transcription. J Biol Chem. [Epub ahead of print]. Application: Rat neuronal cell lysate, acid-extracted. Companys-Alemany J et al. (2020) A Novel NMDA Receptor Antagonist Protects against Cognitive Decline Presented by Senescent Mice. Pharmaceutics. 12(3), 284. Application: Mouse hippocampal homogenates. Furukawa Y et al. (2020) Citrus Auraptene Induces Expression of Brain-Derived Neurotrophic Factor in Neuro2a Cells. Molecules. 25(5). Application: Mouse Neuro2a culture supernatant. Holze F et al. (2019) Distinct acute effects of LSD, MDMA, and D-amphetamine in healthy subjects. Neuropsychopharmacology. [Epub ahead of print]. Application: Human plasma. Sumiyoshi E et al. (2019) Sub-Chronic Consumption of Dark Chocolate Enhances Cognitive Function and Releases Nerve Growth Factors: A Parallel-Group Randomized Trial. Nutrients. 11(11). Application: Human plasma. Sartori A et al. (2019) Interferon-beta, but not Glatiramer Acetate treatment induces gender-specific increase in BDNF serum levels in relapsing-remitting multiple sclerosis female patients. Res J Neuro N Disord. 1:5-18. Application: Human serum. Please refer to our Technical Note #5 for validation experiments disproving the author's claim that the Biosensis Mature BDNF Rapid TM ELISA quantifies total BDNF! Vanicek T et al. (2019) Repetitive Enhancement of Serum BDNF Subsequent to Continuation ECT. Acta Psychiatr Scand. [Epub ahead of print]. Application: Human serum. Gejl AK et al. (2019) Associations between serum and plasma brain-derived neurotrophic factor and influence of storage time and centrifugation strategy. Sci Rep. 9(1):9655. Application: Human serum and EDTA-plasma. Li X et al. (2019) Exercise enhances the expression of brain-derived neurotrophic factor in the hippocampus accompanied by epigenetic alterations in senescence-accelerated mice prone 8. Neurosci Lett. [Epub ahead of print]. Application: Mouse brain homogenates. Yang CY et al. (2019) Panax notoginsenoside Rb1 Restores the Neurotrophic Imbalance Following Photothrombotic Stroke in Rats. Neurotox Res. [Epub ahead of print]. Application: Rat brain homogenates. Du Y et al. (2019) Genome-Wide, Integrative Analysis Implicates Exosome-Derived MicroRNA Dysregulation in Schizophrenia. Schizophr Bull. [Epub ahead of print]. Application: Human serum. Vanicek T et al. (2019) Acute and Subsequent Continuation Electroconvulsive Therapy Elevates Serum BDNF Levels in Patients with Major Depression. Brain Stimul. [In press]. Application: Human serum, plasma. Duart-Castells L et al. (2019) 7,8-dihydroxyflavone blocks the development of behavioral sensitization to MDPV, but not to cocaine: differential role of the BDNF-TrkB pathway. Biochem Pharmacol. [Epub ahead of print]. Application: Mouse RIPA tissue homogenates.
Typical limit of detection (LOD) for BDNF is less than 2 pg/mL, determined as 150% of the blank value.
Cross Reactivity:
No cross-reactivity is observed for nerve growth factor (NGF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL in assay buffer. The reactivity of full-length proBDNF (0.125 ng/mL - 5 ng/mL) was determined in six independent assays using proBDNF proteins from four different sources (mammalian and bacterial, wild-type and mutated). The average cross-reactivity of proBDNF was found to be 5.3% +/- 0.5% in weight (w/v) concentration, or 12.1% +/- 1.2% in molar concentration (mean +/- SEM). Additional proBDNF cross-reactivity experiments were conducted as summarized in our <a class="newA" target="_blank" href="https://www.biosensis.com/documents/enhancedinfo/Technical-Note-5-Mature-BDNF-Isoform-Detection-and-Quantification-by-ELISA.pdf">Technical Note #5</a>.
The Biosensis Mature BDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mature BDNF in less than 3 hours in cell culture supernatants, serum, plasma (citrate and EDTA), pig serum, cell lysates, brain extracts, human milk and Sheep CSF only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Please refer to the kit protocol for specific use instructions for each substrate application, in particular blood samples, human milk and CSF. Note that accurate quantification of BDNF in human milk requires a secretory IgA (sIgA) blocker which can be purchased separately ( BL-001-1250 ). For measurement of mature BDNF in CSF samples, please contact us at sales@biosensis.com . This ELISA kit has been tested in independent research laboratories and found to achieve highest reproducibility with intra- and inter-assay CVs as low as 1% and 5%, respectively (Polacchini et al., 2015). This ELISA kit consists of a pre-coated mouse monoclonal anti-mature BDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature BDNF present in samples and protein standards. A BDNF positive control (QC sample) is provided to assure consistent assay performance. This Mature BDNF ELISA kit employs a recombinant human mature BDNF standard approved by the World Health Organization (WHO, www.nibsc.org ). The amino acid sequence of mature BDNF is identical for human, mouse, rat and a number of other species. This kit therefore is suitable to measure mature BDNF in all these species and uses the same antibodies and antigen. Extensive validation has demonstrated that the Mature BDNF Rapid TM ELISA shows only minimal cross-reactivity with proBDNF. Please refer to our Technical Note #5 for further details on ELISA assay validation for BDNF isoform detection and quanification. This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures. For in-vitro diagnostic (IVD) applications in the European Economic Area (EEA), we refer to the CE Marked BDNF ELISA kit (BEK-2211-CE) .
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human BDNF with an N-terminal methionine residue, made in E. coli (WHO reference reagent)
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, mature (BDNF, mature) in Culture Supernatant, Serum, Plasma (Citrate), Plasma (EDTA), Cell Lysates, Tissue Homogenates, Human Milk, CSF. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Total Number of References: 99 Latest Publications (2019-2022):
Meshkat S et al. (2022) Brain-Derived Neurotrophic Factor (BDNF) as a biomarker of treatment response in patients with Treatment Resistant Depression (TRD): A systematic review & meta-analysis Psychiatry Res. 317:114857 Application: Human, serum. Cook A et al. (2022) Activation of TrkB-Akt signaling rescues deficits in a mouse model of SCA6 Sci Adv. [Epub ahead of print] Application: Mouse, brain extracts. Tsotsoros CE et al. (2022) Pilot Associations between Adverse Childhood Experiences, Executive Function, and Brain-Derived Neurotrophic Factor (BDNF) among Adults with Excess Adiposity Obesities. 2, 276-284. Application: Human, serum. Salem HA et al. (2022) Neuroprotective Effect of Morin Hydrate against Attention-Deficit/Hyperactivity Disorder (ADHD) Induced by MSG and/or Protein Malnutrition in Rat Pups: Effect on Oxidative/Monoamines/Inflammatory Balance and Apoptosis Pharmaceuticals. 15, 1012. Application: Rat, brain supernatant. Aldhshan MS & Mizuno TM. (2022) Effect of environmental enrichment on aggression and the expression of brain-derived neurotrophic factor transcript variants in group-housed male mice Behav Brain Res. [Epub ahead of print]. Application: Mouse, brain tissue homogenate. Fujino M et al. (2022) Orally Administered Plasmalogens Alleviate Negative Mood States and Enhance Mental Concentration: A Randomized, Double-Blind, Placebo-Controlled Trial Front Cell Dev Biol. 10:894734 Application: Human, plasma. Abrial E et al. (2022) Investigating Predictive Factors of Suicidal Re-attempts in Adolescents and Young Adults After a First Suicide Attempt, a Prospective Cohort Study. Study Protocol of the SURAYA Project Front. Psychiatry. [Epub ahead of print] Application: Human, plasma. Tanaka-Kanegae R et al. (2022) Sufficiently Elevated Core Body Temperature May Be Necessary to Maintain Cerebral Blood Flow Response throughout the Morning Neurosci Med. 13, 70-90 Application: Human, serum. Merlo S et al. (2022) Microglial polarization differentially affects neuronal vulnerability to the ?-amyloid protein: Modulation by melatonin Biochem Pharmacol. 202:115151 Application: Human, cell culture supernatant. Dalile B et al. (2022) Extruded Wheat Bran Consumption Increases Serum Short-Chain Fatty Acids but Does Not Modulate Psychobiological Functions in Healthy Men: A Randomized, Placebo-Controlled Trial Front Nutr. 9:896154 Application: Human, serum. Agapouda A et al. (2022) Rhodiola Rosea Extract Counteracts Stress in an Adaptogenic Response Curve Manner via Elimination of ROS and Induction of Neurite Outgrowth Oxid. Med. Cell. Longev. [Epub ahead of print] Application: Human, cell lysates. Breazeale S et al. (2022) Symptom cluster profiles following traumatic orthopaedic injuries Injury. [Epub ahead of print] Application: Human, serum. Jaehne EJ et al. (2022) Behavioral phenotyping of a rat model of the BDNF Val66Met polymorphism reveals selective impairment of fear memory Transl Psychiatry. 12(1):93 Application: Rat, acid extracted tissue lysates. Wang RY et al. (2022) The SDF1-CXCR4 Axis Is Involved in the Hyperbaric Oxygen Therapy-Mediated Neuronal Cells Migration in Transient Brain Ischemic Rats. Int J Mol Sci. 23, 1780 Application: Rat, brain tissue homogenate and serum. Hugues N et al. (2022) Time-Dependent Cortical Plasticity during Moderate-Intensity Continuous Training Versus High-Intensity Interval Training in Rats. Cereb Cortex. [Epub ahead of print] Application: Rat, cortical tissue homogenate. Cefis M et al. (2021) Endothelial cells are an important source of BDNF in rat skeletal muscle. Sci Rep. 12(1):311 Application: Rat, skeletal muscle tissue homogenate. Becker AM et al. (2021) Acute Effects of Psilocybin After Escitalopram or Placebo Pretreatment in a Randomized, Double-Blind, Placebo-Controlled, Crossover Study in Healthy Subjects. Clin Pharmacol Ther. [Epub ahead of print] Application: Human, plasma. Berbenetz N et al. (2021) The Relationship Between Brain Derived Neurotrophic Factor (BDNF) and Symptoms Following Catheter Ablation for Paroxysmal Atrial Fibrillation (AF)- NEURO-AF Study. Circulation. [Epub ahead of print] Application: Human, serum. Walsh JJ et al. (2021) Short-term ketone monoester supplementation improves cerebral blood flow and cognition in obesity: A randomized cross-over trial. J Physiol. [Epub ahead of print] Application: Human, serum, platelet-poor plasma. Boukhatem I et al. (2021) The brain-derived neurotrophic factor prompts platelet aggregation and secretion. Blood Adv. 5(18):3568-3580 Application: Human, plasma. Yi X et al. (2021) Serum mBDNF and ProBDNF Expression Levels as Diagnosis Clue for Early Stage Parkinson's Disease. Front Neurol. 12:680765 Application: Human, serum. Inoue T et al. (2021) Ipsilateral BDNF mRNA expression in the motor cortex positively correlates with motor function of the affected forelimb after intracerebral hemorrhage. Brain Res. [Epub ahead of print] Application: Rat, brain homogenate. Shoshina II et al. (2021) Visual processing and BDNF levels in first-episode schizophrenia. Psychiatry Res. [Epub ahead of print] Application: Human, serum. Cappoli N et al. (2021) Effects of remifentanil on human C20 microglial pro-inflammatory activation. Eur Rev Med Pharmacol Sci. 25(16):5268-5274 Application: Human, cell culture. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print] Application: Human, urine. Mori Y et al. (2021) Serum BDNF as a Potential Biomarker of Alzheimer's Disease: Verification Through Assessment of Serum, Cerebrospinal Fluid, and Medial Temporal Lobe Atrophy. Front Neurol. 12:653267 Application: Human, serum. Seno S et al. (2021) Effects of Selective Serotonin Reuptake Inhibitors on Depression-Like Behavior in a Laser-Induced Shock Wave Model. Front Neurol. 12:602038 Application: Mouse, hippocampal homogenates. Medeiros GC et al. (2021) Treatment of depression with ketamine does not change plasma levels of brain-derived neurotrophic factor or vascular endothelial growth factor. J Affect Disord. 280(Pt A):136-139 Application: Human, plasma. Dorandish S et al. (2021) Differences in the Relative Abundance of ProBDNF and Mature BDNF in A549 and H1299 Human Lung Cancer Cell Media. Int J Mol Sci. 22(13):7059 Application: Human, culture supernatant. Yap NY et al. (2021) Relationship between cytokines and brain-derived neurotrophic factor (BDNF) in trajectories of cancer-related cognitive impairment. Cytokine. [Epub ahead of print] Application: Human, plasma. Wang L et al. (2021) The mediating effect of brain-derived neurotrophic factor levels on childhood trauma and psychiatric symptoms in patients with first-episode schizophrenia. Aust N Z J Psychiatry. [Epub ahead of print] Application: Human. Mallik SB et al. (2021) Remedial effects of caffeine against depressive-like behaviour in mice by modulation of neuroinflammation and BDNF. Nutr Neurosci. [Epub ahead of print] Application: Mouse. Nomura S et al. (2021) Effects of a Tea Cultivar "MK5601" on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice. J Nutr Sci Vitaminol (Tokyo). 67(3):170-179 Application: Mouse, hippocampal RIPA homogenates. Caruso GI et al. (2021) SIRT1-Dependent Upregulation of BDNF in Human Microglia Challenged with A?: An Early but Transient Response Rescued by Melatonin. Biomedicines. 9(5):466 Application: Human, cell culture supernatant. Miller KM et al. (2021) Striatal Afferent BDNF Is Disrupted by Synucleinopathy and Partially Restored by STN DBS. J Neurosci. 41(9):2039-52 Application: Rat, tissue homogenates (RIPA). Vickneson K et al. (2021) Cold-induced dishabituation in rodents exposed to recurrent hypoglycaemia. Diabetologia. 64(6):1436-41 Application: Rat, blood. Li P et al. (2021) Intermediation of perceived stress between early trauma and plasma M/P ratio levels in obsessive-compulsive disorder patients. J Affect Disord. 285:105-111 Application: Human, plasma. Lai NS et al. (2021) Increased Serum Levels of Brain-Derived Neurotrophic Factor Contribute to Inflammatory Responses in Patients with Rheumatoid Arthritis. Int. J. Mol. Sci. 22(4):1841 Application: Human, serum and culture supernatants. Pan S et al. (2021) The microRNA-195 - BDNF pathway and cognitive deficits in schizophrenia patients with minimal antipsychotic medication exposure. Transl Psychiatry. 11(1):117 Application: Human, plasma. Miyamoto T et al. (2021) Effect of pedaling cadence on serum levels of brain-derived neurotrophic factor during ergometric exercise in healthy adults. Sport Sci Health. Application: Human, serum. Normann AJ (2020) The Effect of Light Therapy and Acute Aerobic Exercise on Serum Brain Derived Neurotrophic Factor in Older Adults. MSc Thesis. Application: Human, serum. Holze F et al. (2020) Acute dose-dependent effects of lysergic acid diethylamide in a double-blind placebo-controlled study in healthy subjects. Neuropsychopharmacology. [Epub ahead of print]. Application: Human, plasma. Hasler G et al. (2020) The Association Between Adolescent Residential Mobility and Adult Social Anxiety, BDNF and Amygdala-Orbitofrontal Functional Connectivity in Young Adults With Higher Education. Front. Psychiatry. Application: Human, serum. Wallace AW (2020) The Impact of Six Weeks of Intermittent Fasting, With and Without Aerobic Exercise, on Serum BDNF in Young Adult Males. MSc Thesis. Application: Human, serum. Walsh JJ et al. (2020) The Effect of Exogenous Ketone Monoester Ingestion on Plasma BDNF During an Oral Glucose Tolerance Test. Front Physiol. 11:1094. Application: Human, plasma. Meade GM et al. (2020) A Model of Negative Emotional Contagion Between Male-Female Rat Dyads: Effects of Voluntary Exercise on Stress-Induced Behavior and BDNF-TrkB Signaling. Physiol Behav. 113286 Application: Rat, serum. Hutten NRPW et al. (2020) Low Doses of LSD Acutely Increase BDNF Blood Plasma Levels in Healthy Volunteers. ACS Pharmacol. Transl. Sci. Application: Human, plasma. Barbosa AC et al. (2020) Assessment of BDNF serum levels as a diagnostic marker in children with autism spectrum disorder. Sci Rep. 10(1):17348. Application: Human, serum. Okamura M et al. (2020) Low-Level Inhibition of GABAergic Synapses Enhances Gene Expressions Crucial for Neuronal Plasticity in the Hippocampus After Ischemic Stroke. J Stroke Cerebrovasc Dis. 29(12):105316. Application: Rat, hippocampus homogenate. Payne AJ et al. (2020) The Effects of Alcohol on BDNF and CD5 Dependent Pathways. PhD Thesis. Application: Mouse, RIPA tissue homogenate. Nishiyama M et al. (2020) Homostachydrine is a Xenobiotic Substrate of OCTN1/SLC22A4 and Potentially Sensitizes Pentylenetetrazole-Induced Seizures in Mice. Neurochem Res. [Epub ahead of print]. Application: Mouse, acid-extracted hippocampal homogenate. Lorinczova HT et al. (2020) Co-Administration of Iron and a Bioavailable Curcumin Supplement Increases Serum BDNF Levels in Healthy Adults. Antioxidants (Basel). 9(8):E645. Application: Human serum. Yap NY F et al. (2020) Associations of plasma brain-derived neurotrophic factor (BDNF) and Val66Met polymorphism (rs6265) with long-term cancer-related cognitive impairment in survivors of breast cancer. Breast Cancer Res Treat. [Epub ahead of print]. Application: Human plasma. Vasilopoulou F et al. (2020) Amelioration of BPSD-Like Phenotype and Cognitive Decline in SAMP8 Mice Model Accompanied by Molecular Changes After Treatment With I 2-Imidazoline Receptor Ligand MCR5. Pharmaceutics. 12(5):E475. Application: Mouse hippocampus RIPA-homogenates. Mueller ST et al. (2020) Negative Association Between Left Prefrontal GABA Concentration and BDNF Serum Concentration in Young Adults. Heliyon. 6(5):e04025. Application: Human serum. Chen LF et al. (2020) The NMDA receptor subunit GluN3A regulates synaptic activity-induced and myocyte enhancer factor 2C (MEF2C)-dependent transcription. J Biol Chem. [Epub ahead of print]. Application: Rat neuronal cell lysate, acid-extracted. Companys-Alemany J et al. (2020) A Novel NMDA Receptor Antagonist Protects against Cognitive Decline Presented by Senescent Mice. Pharmaceutics. 12(3), 284. Application: Mouse hippocampal homogenates. Furukawa Y et al. (2020) Citrus Auraptene Induces Expression of Brain-Derived Neurotrophic Factor in Neuro2a Cells. Molecules. 25(5). Application: Mouse Neuro2a culture supernatant. Holze F et al. (2019) Distinct acute effects of LSD, MDMA, and D-amphetamine in healthy subjects. Neuropsychopharmacology. [Epub ahead of print]. Application: Human plasma. Sumiyoshi E et al. (2019) Sub-Chronic Consumption of Dark Chocolate Enhances Cognitive Function and Releases Nerve Growth Factors: A Parallel-Group Randomized Trial. Nutrients. 11(11). Application: Human plasma. Sartori A et al. (2019) Interferon-beta, but not Glatiramer Acetate treatment induces gender-specific increase in BDNF serum levels in relapsing-remitting multiple sclerosis female patients. Res J Neuro N Disord. 1:5-18. Application: Human serum. Please refer to our Technical Note #5 for validation experiments disproving the author's claim that the Biosensis Mature BDNF Rapid TM ELISA quantifies total BDNF! Vanicek T et al. (2019) Repetitive Enhancement of Serum BDNF Subsequent to Continuation ECT. Acta Psychiatr Scand. [Epub ahead of print]. Application: Human serum. Gejl AK et al. (2019) Associations between serum and plasma brain-derived neurotrophic factor and influence of storage time and centrifugation strategy. Sci Rep. 9(1):9655. Application: Human serum and EDTA-plasma. Li X et al. (2019) Exercise enhances the expression of brain-derived neurotrophic factor in the hippocampus accompanied by epigenetic alterations in senescence-accelerated mice prone 8. Neurosci Lett. [Epub ahead of print]. Application: Mouse brain homogenates. Yang CY et al. (2019) Panax notoginsenoside Rb1 Restores the Neurotrophic Imbalance Following Photothrombotic Stroke in Rats. Neurotox Res. [Epub ahead of print]. Application: Rat brain homogenates. Du Y et al. (2019) Genome-Wide, Integrative Analysis Implicates Exosome-Derived MicroRNA Dysregulation in Schizophrenia. Schizophr Bull. [Epub ahead of print]. Application: Human serum. Vanicek T et al. (2019) Acute and Subsequent Continuation Electroconvulsive Therapy Elevates Serum BDNF Levels in Patients with Major Depression. Brain Stimul. [In press]. Application: Human serum, plasma. Duart-Castells L et al. (2019) 7,8-dihydroxyflavone blocks the development of behavioral sensitization to MDPV, but not to cocaine: differential role of the BDNF-TrkB pathway. Biochem Pharmacol. [Epub ahead of print]. Application: Mouse RIPA tissue homogenates.
Typical limit of detection (LOD) for BDNF is less than 2 pg/mL, determined as 150% of the blank value.
Cross Reactivity:
No cross-reactivity is observed for nerve growth factor (NGF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL in assay buffer. The reactivity of full-length proBDNF (0.125 ng/mL - 5 ng/mL) was determined in six independent assays using proBDNF proteins from four different sources (mammalian and bacterial, wild-type and mutated). The average cross-reactivity of proBDNF was found to be 5.3% +/- 0.5% in weight (w/v) concentration, or 12.1% +/- 1.2% in molar concentration (mean +/- SEM). Additional proBDNF cross-reactivity experiments were conducted as summarized in our <a class="newA" target="_blank" href="https://www.biosensis.com/documents/enhancedinfo/Technical-Note-5-Mature-BDNF-Isoform-Detection-and-Quantification-by-ELISA.pdf">Technical Note #5</a>.
The Biosensis proBDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, cell lysates, serum, citrate-plasma and tissue extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples. This ELISA kit consists of a pre-coated polyclonal anti-proBDNF capture antibody, a biotinylated anti-matureBDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proBDNF present in samples and protein standards. A proBDNF positive control (QC sample) is provided to assure consistent assay performance. This proBDNF ELISA kit employs a recombinant, cleavage-resistant human proBDNF standard produced by Biosensis and validated against externally available proBDNF proteins. Due to a high degree of amino acid sequence homology, mouse and rat proBDNF can be quantified and expressed as human proBDNF equivalents. Internal Biosensis validation suggests that the use of the human standard provided in this kit will provide estimates that are identical, or close, to the actual levels of rat and mouse proBDNF present in rodent samples. Note that accurate proBDNF quantification in human serum and citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately . This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human proBDNF, mutated to be cleavage-resistant, made in 293F cells
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, pro- (proBDNF) in Culture Supernatant, Cell Lysates, Serum, Plasma (Citrate), Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate, protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Aldhshan MS & Mizuno TM. (2022) "Effect of environmental enrichment on aggression and the expression of brain-derived neurotrophic factor transcript variants in group-housed male mice." Behav Brain Res. [Epub ahead of print]. Application: Brain tissue homogenate. Dorandish S et al. (2021) "Differences in the Relative Abundance of ProBDNF and Mature BDNF in A549 and H1299 Human Lung Cancer Cell Media." Int J Mol Sci. 22(13):7059. Application: Human culture supernatant. Payne AJ (2020) "The Effects of Alcohol on BDNF and CD5 Dependent Pathways." PhD Thesis. Application: Mouse RIPA tissue homogenates. Companys-Alemany J et al. (2020) "A Novel NMDA Receptor Antagonist Protects against Cognitive Decline Presented by Senescent Mice." Pharmaceutics. 12(3), 284. Application: Mouse hippocampal homogenates. Duart-Castells L et al. (2019) "7,8-dihydroxyflavone blocks the development of behavioral sensitization to MDPV, but not to cocaine: differential role of the BDNF-TrkB pathway." Biochem Pharmacol. [Epub ahead of print]. Application: Mouse RIPA tissue homogenates. Osborne A, Wang AX, Tassoni A, Widdowson PS, Martin KR (2018) "Design of a Novel Gene Therapy Construct to Achieve Sustained Brain-Derived Neurotrophic Factor Signalling in Neurons." Hum Gene Ther. [Epub ahead of print]. Application: Human cell line supernatant. Rahman MS, Millischer V, Zeebari Z, Forsell Y, Lavebratt C (2017) "BDNF Val66Met and childhood adversity on response to physical exercise and internet-based cognitive behavioural therapy in depressed Swedish adults." J Psychiatr Res. 93:50-58. Application: Human serum. Riffault B, Kourdougli N, Dumon C, Ferrand N, Buhler E, Schaller F, Chambon C, Rivera C, Gaiarsa JL, Porcher C (2016) "Pro-Brain-Derived Neurotrophic Factor (proBDNF)-Mediated p75NTR Activation Promotes Depolarizing Actions of GABA and Increases Susceptibility to Epileptic Seizures". Cereb. Cortex [Epub ahead of print]. Application: Rat cortex and hippocampus RIPA extracts. Hashimoto T, Shiina A, Hasegawa T, Kimura H, Oda Y, Niitsu T, Ishikawa M, Tachibana M, Muneoka K, Matsuki S, Nakazato M, Iyo M (2016) "Effect of mirtazapine versus selective serotonin reuptake inhibitors on benzodiazepine use in patients with major depressive disorder: a pragmatic, multicenter, open-label, randomized, active-controlled, 24-week trial." Ann Gen Psychiatry. 15(27). Application: Human serum. Niimi M, Hashimoto K, Kakuda W, Miyano S, Momosaki R, Ishima T, Abo M. (2016) "Role of Brain-Derived Neurotrophic Factor in Beneficial Effects of Repetitive Transcranial Magnetic Stimulation for Upper Limb Hemiparesis after Stroke." PLoS One. 11(3):e0152241. Application: Human serum. Stary CM, Sun X, Giffard RG (2015) "Astrocytes Protect against Isoflurane Neurotoxicity by Buffering pro-brain-derived Neurotrophic Factor." Anesthesiology. 123(4):810-9. Application: Rat neuron and astrocyte cell culture supernatant. Riffault B, Medina I, Dumon C, Thalman C, Ferrand N, Friedel P, Gaiarsa JL, Porcher C. (2014) "Pro-Brain-Derived Neurotrophic Factor Inhibits GABAergic Neurotransmission by Activating Endocytosis and Repression of GABAA Receptors." J. Neurosci. 34(40):13516-34. Application: Rat hippocampal culture supernatant.
Typical limit of detection (LOD) for proBDNF is 10 pg/mL determined as 150% of the blank value.
Cross Reactivity:
A cross-reactivity of 2% in weight concentration (0.9% in molar concentration) has been observed for mature BDNF assayed at 25 ng/mL (893 pmol/L) in Assay Diluent A.<br>Due to a high degree of sequence homology, this human proBDNF ELISA kit cross-reacts with the mouse and rat form of proBDNF. Other species have not yet been tested, but cross-reactivity with a wide range of mammalian forms of proBDNF is expected.<br> The antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
The Biosensis proBDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, human serum and EDTA-plasma only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples. This ELISA kit consists of a pre-coated monoclonal anti-proBDNF capture antibody, a biotinylated anti-matureBDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proBDNF present in samples and protein standards. A proBDNF positive control (QC sample) is provided to assure consistent assay performance. This proBDNF ELISA kit employs a recombinant, cleavage-resistant human proBDNF standard produced in mammalian cells by Biosensis and validated against externally available proBDNF proteins. Note that accurate proBDNF quantification in human serum and EDTA-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately . This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Human recombinant proBDNF, mutated to be cleavage-resistant, made in 293F cells
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, pro- (proBDNF) in Culture Supernatant, Serum, Plasma (Citrate), Plasma (EDTA), Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Tsotsoros CE et al. (2022) Pilot Associations between Adverse Childhood Experiences, Executive Function, and Brain-Derived Neurotrophic Factor (BDNF) among Adults with Excess Adiposity Obesities. 2, 276-284. Application: Human, serum. Freidle M et al. (2022) Behavioural and neuroplastic effects of a double-blind randomised controlled balance exercise trial in people with Parkinson's disease. NPJ Parkinsons Dis. 8(1):12. Application: Human, serum. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human, urine. Yi X et al. (2021) Serum mBDNF and ProBDNF Expression Levels as Diagnosis Clue for Early Stage Parkinson's Disease. Front Neurol. 12:680765. Application: Human, serum. Nomura S et al. (2021) Effects of a Tea Cultivar "MK5601" on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice. J Nutr Sci Vitaminol (Tokyo). 67(3):170-179. Application: Mouse, hippocampal RIPA homogenates. Li P et al. (2021) Intermediation of perceived stress between early trauma and plasma M/P ratio levels in obsessive-compulsive disorder patients. J Affect Disord. 285:105-111 Application: Human, plasma. Lai NS et al. (2021) Increased Serum Levels of Brain-Derived Neurotrophic Factor Contribute to Inflammatory Responses in Patients with Rheumatoid Arthritis. Int. J. Mol. Sci. 22(4):1841 Application: Human, serum and culture supernatants. Fukumoto M et al. (2019) Induction of brain-derived neurotrophic factor in enteric glial cells stimulated by interleukin-1? via a c-Jun N-terminal kinase pathway. J. Clin. Biochem. Nutr. [Epub ahead of print]. Application: Human, culture supernatant.
Specificity:
Human proBDNF. The capture antibody used in this ELISA kit binds to an epitope within the pro-domain of proBDNF. Thus, this ELISA detects the full length form of proBDNF and does not quantify mature BDNF. Whether this ELISA kit can detect truncated BDNF is unknown at present. Mature BDNF cross-reactivity was assessed by spiking 28 kDa mature BDNF protein obtained from WHO (www.nibsc.org) into a 1/5 diluted human serum sample at 5 ng/mL, which represents a BDNF concentration level of 25 ng/mL in undiluted, normal human serum. Cross-reactivity of mature BDNF was The assay antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
Typical limit of detection (LOD) for proBDNF is 6 pg/mL determined as 150% of the blank value.
Cross Reactivity:
Mature BDNF cross-reactivity was assessed by spiking 28 kDa mature BDNF protein obtained from WHO (www.nibsc.org) into a 1/5 diluted human serum sample at 5 ng/mL, which represents a BDNF concentration level of 25 ng/mL in undiluted, normal human serum. Cross-reactivity of mature BDNF was < 0.3% (w/v), or < 0.1% in molar concentration.<br><br>The assay antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
The Biosensis proBDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, human serum and EDTA-plasma only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples. This ELISA kit consists of a pre-coated monoclonal anti-proBDNF capture antibody, a biotinylated anti-matureBDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proBDNF present in samples and protein standards. A proBDNF positive control (QC sample) is provided to assure consistent assay performance. This proBDNF ELISA kit employs a recombinant, cleavage-resistant human proBDNF standard produced in mammalian cells by Biosensis and validated against externally available proBDNF proteins. Note that accurate proBDNF quantification in human serum and EDTA-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately . This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Human recombinant proBDNF, mutated to be cleavage-resistant, made in 293F cells
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, pro- (proBDNF) in Culture Supernatant, Serum, Plasma (Citrate), Plasma (EDTA), Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Tsotsoros CE et al. (2022) Pilot Associations between Adverse Childhood Experiences, Executive Function, and Brain-Derived Neurotrophic Factor (BDNF) among Adults with Excess Adiposity Obesities. 2, 276-284. Application: Human, serum. Freidle M et al. (2022) Behavioural and neuroplastic effects of a double-blind randomised controlled balance exercise trial in people with Parkinson's disease. NPJ Parkinsons Dis. 8(1):12. Application: Human, serum. March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human, urine. Yi X et al. (2021) Serum mBDNF and ProBDNF Expression Levels as Diagnosis Clue for Early Stage Parkinson's Disease. Front Neurol. 12:680765. Application: Human, serum. Nomura S et al. (2021) Effects of a Tea Cultivar "MK5601" on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice. J Nutr Sci Vitaminol (Tokyo). 67(3):170-179. Application: Mouse, hippocampal RIPA homogenates. Li P et al. (2021) Intermediation of perceived stress between early trauma and plasma M/P ratio levels in obsessive-compulsive disorder patients. J Affect Disord. 285:105-111 Application: Human, plasma. Lai NS et al. (2021) Increased Serum Levels of Brain-Derived Neurotrophic Factor Contribute to Inflammatory Responses in Patients with Rheumatoid Arthritis. Int. J. Mol. Sci. 22(4):1841 Application: Human, serum and culture supernatants. Fukumoto M et al. (2019) Induction of brain-derived neurotrophic factor in enteric glial cells stimulated by interleukin-1? via a c-Jun N-terminal kinase pathway. J. Clin. Biochem. Nutr. [Epub ahead of print]. Application: Human, culture supernatant.
Specificity:
Human proBDNF. The capture antibody used in this ELISA kit binds to an epitope within the pro-domain of proBDNF. Thus, this ELISA detects the full length form of proBDNF and does not quantify mature BDNF. Whether this ELISA kit can detect truncated BDNF is unknown at present. Mature BDNF cross-reactivity was assessed by spiking 28 kDa mature BDNF protein obtained from WHO (www.nibsc.org) into a 1/5 diluted human serum sample at 5 ng/mL, which represents a BDNF concentration level of 25 ng/mL in undiluted, normal human serum. Cross-reactivity of mature BDNF was The assay antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
Typical limit of detection (LOD) for proBDNF is 6 pg/mL determined as 150% of the blank value.
Cross Reactivity:
Mature BDNF cross-reactivity was assessed by spiking 28 kDa mature BDNF protein obtained from WHO (www.nibsc.org) into a 1/5 diluted human serum sample at 5 ng/mL, which represents a BDNF concentration level of 25 ng/mL in undiluted, normal human serum. Cross-reactivity of mature BDNF was < 0.3% (w/v), or < 0.1% in molar concentration.<br><br>The assay antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
The Biosensis proBDNF Rapid TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, cell lysates, serum, citrate-plasma and tissue extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples. This ELISA kit consists of a pre-coated polyclonal anti-proBDNF capture antibody, a biotinylated anti-matureBDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proBDNF present in samples and protein standards. A proBDNF positive control (QC sample) is provided to assure consistent assay performance. This proBDNF ELISA kit employs a recombinant, cleavage-resistant human proBDNF standard produced by Biosensis and validated against externally available proBDNF proteins. Due to a high degree of amino acid sequence homology, mouse and rat proBDNF can be quantified and expressed as human proBDNF equivalents. Internal Biosensis validation suggests that the use of the human standard provided in this kit will provide estimates that are identical, or close, to the actual levels of rat and mouse proBDNF present in rodent samples. Note that accurate proBDNF quantification in human serum and citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately . This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
Background Info:
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
ELISA Assay
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human proBDNF, mutated to be cleavage-resistant, made in 293F cells
Applications:
ELISA
Application Details:
ELISA. For the quantification of Brain-derived neurotrophic factor, pro- (proBDNF) in Culture Supernatant, Cell Lysates, Serum, Plasma (Citrate), Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
The ELISA kit box contains 96-well pre-coated strip plates, protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Product references:
Aldhshan MS & Mizuno TM. (2022) "Effect of environmental enrichment on aggression and the expression of brain-derived neurotrophic factor transcript variants in group-housed male mice." Behav Brain Res. [Epub ahead of print]. Application: Brain tissue homogenate. Dorandish S et al. (2021) "Differences in the Relative Abundance of ProBDNF and Mature BDNF in A549 and H1299 Human Lung Cancer Cell Media." Int J Mol Sci. 22(13):7059. Application: Human culture supernatant. Payne AJ (2020) "The Effects of Alcohol on BDNF and CD5 Dependent Pathways." PhD Thesis. Application: Mouse RIPA tissue homogenates. Companys-Alemany J et al. (2020) "A Novel NMDA Receptor Antagonist Protects against Cognitive Decline Presented by Senescent Mice." Pharmaceutics. 12(3), 284. Application: Mouse hippocampal homogenates. Duart-Castells L et al. (2019) "7,8-dihydroxyflavone blocks the development of behavioral sensitization to MDPV, but not to cocaine: differential role of the BDNF-TrkB pathway." Biochem Pharmacol. [Epub ahead of print]. Application: Mouse RIPA tissue homogenates. Osborne A, Wang AX, Tassoni A, Widdowson PS, Martin KR (2018) "Design of a Novel Gene Therapy Construct to Achieve Sustained Brain-Derived Neurotrophic Factor Signalling in Neurons." Hum Gene Ther. [Epub ahead of print]. Application: Human cell line supernatant. Rahman MS, Millischer V, Zeebari Z, Forsell Y, Lavebratt C (2017) "BDNF Val66Met and childhood adversity on response to physical exercise and internet-based cognitive behavioural therapy in depressed Swedish adults." J Psychiatr Res. 93:50-58. Application: Human serum. Riffault B, Kourdougli N, Dumon C, Ferrand N, Buhler E, Schaller F, Chambon C, Rivera C, Gaiarsa JL, Porcher C (2016) "Pro-Brain-Derived Neurotrophic Factor (proBDNF)-Mediated p75NTR Activation Promotes Depolarizing Actions of GABA and Increases Susceptibility to Epileptic Seizures". Cereb. Cortex [Epub ahead of print]. Application: Rat cortex and hippocampus RIPA extracts. Hashimoto T, Shiina A, Hasegawa T, Kimura H, Oda Y, Niitsu T, Ishikawa M, Tachibana M, Muneoka K, Matsuki S, Nakazato M, Iyo M (2016) "Effect of mirtazapine versus selective serotonin reuptake inhibitors on benzodiazepine use in patients with major depressive disorder: a pragmatic, multicenter, open-label, randomized, active-controlled, 24-week trial." Ann Gen Psychiatry. 15(27). Application: Human serum. Niimi M, Hashimoto K, Kakuda W, Miyano S, Momosaki R, Ishima T, Abo M. (2016) "Role of Brain-Derived Neurotrophic Factor in Beneficial Effects of Repetitive Transcranial Magnetic Stimulation for Upper Limb Hemiparesis after Stroke." PLoS One. 11(3):e0152241. Application: Human serum. Stary CM, Sun X, Giffard RG (2015) "Astrocytes Protect against Isoflurane Neurotoxicity by Buffering pro-brain-derived Neurotrophic Factor." Anesthesiology. 123(4):810-9. Application: Rat neuron and astrocyte cell culture supernatant. Riffault B, Medina I, Dumon C, Thalman C, Ferrand N, Friedel P, Gaiarsa JL, Porcher C. (2014) "Pro-Brain-Derived Neurotrophic Factor Inhibits GABAergic Neurotransmission by Activating Endocytosis and Repression of GABAA Receptors." J. Neurosci. 34(40):13516-34. Application: Rat hippocampal culture supernatant.
Typical limit of detection (LOD) for proBDNF is 10 pg/mL determined as 150% of the blank value.
Cross Reactivity:
A cross-reactivity of 2% in weight concentration (0.9% in molar concentration) has been observed for mature BDNF assayed at 25 ng/mL (893 pmol/L) in Assay Diluent A.<br>Due to a high degree of sequence homology, this human proBDNF ELISA kit cross-reacts with the mouse and rat form of proBDNF. Other species have not yet been tested, but cross-reactivity with a wide range of mammalian forms of proBDNF is expected.<br> The antibodies do not cross-react with nerve growth factor (NGF), neurotrophin-3 (NT-3) or NT-4/5.
BRI1 (Protein BRASSINOSTEROID INSENSITIVE 1) is a receptor which binds brassinolide and has a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates. Involved in a signaling cascade including expression of light- and stress-regulated genes, promotion of cell elongation, normal leaf and chloroplast senescence, and flowering. Alternative names:BRI1, BRASSINOSTEROID INSENSITIVE 1, CBB2, CABBAGE 2, DWF2, DWARF 2, BIN1, BR INSENSITIVE 1, ATBRI1, Brassinosteroid LRR receptor kinase
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus, Brassica rapaSpecies of your interest not listed? Contact us
Antibody was tested on bri1-1 and bri1-5 mutants. Bri1-1 is a point mutation in the kinase domain that renders the protein non-functional and plants compensate for that by over-accumulating the non-functional receptor. Bri1-5 is a mutant in the extracellular domain and the bri1-5 protein is retained in the ER. The bri1-5 plants contain less protein than the wild type and show an intermediate brassinosteroid deficient phenotype. Also BRI1-5 migrates higher than wild type BRI1 in SDS-PAGE, because it carries ER-type high mannose N-glycans. For IP: 15 l GFP-trap beads was used for 200 mg plant material to precipitate GFP-tagged protein followed by detection with Co-IPed BRI1 on Western with 1:5000 diluted anti-BRI1 antibody.Protein extraction has to be done efficiently as this step is crucial, recommended material to buffer ratio: 15 l/ g or less.
Lee et al (2021). Chaperone-like protein DAY plays critical roles in photomorphogenesis. Nat Commun. 2021 Jul 7;12(1):4194. doi: 10.1038/s41467-021-24446-5. PMID: 34234144; PMCID: PMC8263706.Chen et al. (2019). BES1 is activated by EMS1-TPD1-SERK1/2-mediated signaling to control tapetum development in Arabidopsis thaliana. Nat Commun. 2019 Sep 13;10(1):4164. doi: 10.1038/s41467-019-12118-4.Hou et al. (2019). Less Conserved LRRs Is Important for BRI1 Folding. Front Plant Sci. 2019 May 21;10:634. doi: 10.3389/fpls.2019.00634. eCollection 2019.Chen et al. (2019). BZR1 Family Transcription Factors Function Redundantly and Indispensably in BR Signaling but Exhibit BRI1-Independent Function in Regulating Anther Development in Arabidopsis. Mol Plant. 2019 Jun 20. pii: S1674-2052(19)30207-2. doi: 10.1016/j.molp.2019.06.006.
Special application note:
This product can be sold containing proclin if requested
The BrightDAB substrate DAB (3,3Diaminobenzidine) is a widely used chromogen for immunohistochemical staining and immunoblotting. When in the presence of peroxidase enzyme, DAB produces a brown precipitate that is insoluble in alcohol and xylene. This product comes in a two-component system consisting of a liquid stable DAB chromogen and DAB substrate buffer. DAB Solution A: Readyto-Use Buffered H2O2,; DAB Solution B: Concentrated DAB solution. BrightDAB is a very stable and superior formulation of DAB. In some cases, antibodies titers may increase by two-fold. BrightDAB can be used both manually and on automated stainers. The clinical interpretation of any staining or its absence should be determined by a qualified pathologist and complemented by morphologic studies; controls should be evaluated within the context of the patients clinical history and/or other diagnostic tests. Also available in 500 ml and 1000 ml.
Principle of method:
DAB substrate for use with HRP-labeled detection systems
Reagents provided:
DAB Solution A: Buffered H2O2 (Ready-to-Use) 110 ml DAB Solution B: Concentrated DAB solution 5 ml
Storage and handling:
2-8°C and in the dark
Reagent preparation:
1 Working solution: Add 40 ?l DAB Solution B (± one drop) to 1 ml Solution A, mix well. 2 Incubate the DAB solution one time 8 minutes without washing in between.
Procedure:
1. Deparaffinize and rehydrate tissue section (slide/tissue peparing), 2. Wash Aqua dest (Wash; 2x 5 min), 3. If applicable, HIER or digestive enzyme (pre-treatment), 4. Wash buffer (PBS or TBS buffer; 2x 5 min), 5. H2O2 (conc3%) (Tissue preparing; 10 min), 6. Wash buffer (PBS or TBS buffer; 2x 5 min), 7. Primary mouse or rabbit antibody (Antibody; 30 min), 8. Wash buffer (PBS or TBS buffer; 2x 5 min), 9. Detection system, polymer HRP, (Labeled polymer; 30 min), 10. Wash buffer (PBS or TBS buffer; 2x 5 min), 11. Substrate (DAB; 8 min), 12. Wash aqua dest (Wash; 2x 2 min), 13. Counterstain, dehydrate and coverslip (Auxiliary)
Quality Control:
A positive control, negative control and reagent control are needed and processed in the same way as the unknow specimen slide to interpret staining results.
The BrightDiluents universal IHC diluent is specially formulated to stabilize antibodies and eliminate backgrounds encountered in immunohistochemistry staining procedures. The diluent contains complex proteins and non-protein mixtures which eliminate histostaining background by blocking Fc receptors and prevents non-specific protein-protein interactions. It can also be used as blocking applied before primary antibodies. If primary antibodies are diluted in this diluent, a blocking step before primary antibodies can generally be omitted. The blocking /diluent solution is supplied in Ready-to-Use format. It should not be diluted to be effective. The diluent contains no azide, therefore, it can be used to dilute and stabilize HRP-conjugates as well as any other solution that need and stabilizing carrier protein, such as Blocking and Poly-AP conjugates. This product should be interpreted by a qualified pathologist with relevant clinical information, morphological and histological studies and with proper controls. The ready-to-use antibody diluent is available in different colors: MON-APP917 (Transparant/colorless), MON-APP917B (Blue), MON-APP917Y (Yellow), MON-APP917G (green), MON-APP917R (Red)
Principle of method:
Antibody diluent
Reagents provided:
125 ml BrightDiluent, normal antibody diluent (ready-to-use)
The BrightDiluents universal IHC diluent is specially formulated to stabilize antibodies and eliminate backgrounds encountered in immunohistochemistry staining procedures. The diluent contains complex proteins and non-protein mixtures which eliminate histostaining background by blocking Fc receptors and prevents non-specific protein-protein interactions. It can also be used as blocking applied before primary antibodies. If primary antibodies are diluted in this diluent, a blocking step before primary antibodies can generally be omitted. The blocking /diluent solution is supplied in Ready-to-Use format. It should not be diluted to be effective. The diluent contains no azide, therefore, it can be used to dilute and stabilize HRP-conjugates as well as any other solution that need and stabilizing carrier protein, such as Blocking and Poly-AP conjugates. This product should be interpreted by a qualified pathologist with relevant clinical information, morphological and histological studies and with proper controls. The ready-to-use antibody diluent is available in different colors: MON-APP917 (Transparant/colorless), MON-APP917B (Blue), MON-APP917Y (Yellow), MON-APP917G (green), MON-APP917R (Red)
Principle of method:
Antibody diluent
Reagents provided:
125 ml BrightDiluent, normal antibody diluent (ready-to-use)
The BrightDiluents universal IHC diluent is specially formulated to stabilize antibodies and eliminate backgrounds encountered in immunohistochemistry staining procedures. The diluent contains complex proteins and non-protein mixtures which eliminate histostaining background by blocking Fc receptors and prevents non-specific protein-protein interactions. It can also be used as blocking applied before primary antibodies. If primary antibodies are diluted in this diluent, a blocking step before primary antibodies can generally be omitted. The blocking /diluent solution is supplied in Ready-to-Use format. It should not be diluted to be effective. The diluent contains no azide, therefore, it can be used to dilute and stabilize HRP-conjugates as well as any other solution that need and stabilizing carrier protein, such as Blocking and Poly-AP conjugates. This product should be interpreted by a qualified pathologist with relevant clinical information, morphological and histological studies and with proper controls. The ready-to-use antibody diluent is available in different colors: MON-APP917 (Transparant/colorless), MON-APP917B (Blue), MON-APP917Y (Yellow), MON-APP917G (green), MON-APP917R (Red)
Principle of method:
Antibody diluent
Reagents provided:
125 ml BrightDiluent, normal antibody diluent (ready-to-use)
The BrightDiluents universal IHC diluent is specially formulated to stabilize antibodies and eliminate backgrounds encountered in immunohistochemistry staining procedures. The diluent contains complex proteins and non-protein mixtures which eliminate histostaining background by blocking Fc receptors and prevents non-specific protein-protein interactions. It can also be used as blocking applied before primary antibodies. If primary antibodies are diluted in this diluent, a blocking step before primary antibodies can generally be omitted. The blocking /diluent solution is supplied in Ready-to-Use format. It should not be diluted to be effective. The diluent contains no azide, therefore, it can be used to dilute and stabilize HRP-conjugates as well as any other solution that need and stabilizing carrier protein, such as Blocking and Poly-AP conjugates. This product should be interpreted by a qualified pathologist with relevant clinical information, morphological and histological studies and with proper controls. The ready-to-use antibody diluent is available in different colors: MON-APP917 (Transparant/colorless), MON-APP917B (Blue), MON-APP917Y (Yellow), MON-APP917G (green), MON-APP917R (Red)
Principle of method:
Antibody diluent
Reagents provided:
125 ml BrightDiluent, normal antibody diluent (ready-to-use)
For in-vitro Diagnostic Use. The BrightVision one step detection system peroxidase Goat Anti-Mouse IgG AP, is intended for use in immunohistochemistry for the detection of mouse antibodies.
The BrightVision detection system Goat Anti- Mouse AP, is a Ready-to-Use system that has been manufactured to give an optimal staining, when using the protocol advised in this IFU. Prior to staining some routine fixed, paraffin-embedding tissue sections should be subjected to pre-treatment (HIER or digestive enzyme). The BrightVision detection system detects Mouse bound to an antigen in tissue sections. This polymer-complex is then visualized with a suitable substrate/chromogen (not provided). Also available in 110 ml, 500 ml and 1000 ml.
Principle of method:
One step detection system peroxidase goat anti-mouse IgG AP
Reagents provided:
One step detection system Goat anti-Mouse AP (Ready-to-use; 55 ml)
Storage and handling:
2-8°C and in the dark
Procedure:
1. Deparaffinize and rehydrate tissue section (slide/tissue peparing), 2. Wash Aqua dest (Wash; 2x 5 min), 3. If applicable, HIER or digestive enzyme (pre-treatment), 4. Wash buffer (PBS or TBS buffer; 2x 5 min), 5. H2O2 (conc3%) (Tissue preparing; 10 min), 6. Wash buffer (PBS or TBS buffer; 2x 5 min), 7. Primary mouse antibody (Antibody; 30 min), 8. Wash buffer (TBS buffer; 2x 5 min), 9. Detection system, polymer Mouse AP, (Labeled polymer; 30 min), 10. Wash buffer (TBS buffer; 2x 5 min), 11. Substrate (FAST Red / New Fuchsin; see applicable IFU), 12. Wash aqua dest (Wash; 2x 2 min), 13. Counterstain and coverslip with a aqueous mounting medium (Auxiliary)
Quality Control:
A positive control, negative control and reagent control are needed and processed in the same way as the unknow specimen slide to interpret staining results.
For in-vitro Diagnostic Use. The BrightVision one step detection system Goat Anti-Mouse IgG HRP and Goat anti-rabbit IgG AP, is intended for use in immunohistochemistry for the detection of mouse or rabbit antibodies.
The BrightVision detection system, peroxidase Goat Anti-Mouse HRP/Goat anti-Rabbit AP, is a Ready-to-Use system that has been manufactured to give an optimal staining, when using the protocol advised in this IFU. Prior to staining some routine fixed, paraffin-embedding tissue sections should be subjected to pre-treatment (HIER or digestive enzyme). The BrightVision detection system detects Rabbit and Mouse bound to an antigen in tissue sections. This polymer-complex is then visualized with a suitable substrate/chromogen. The clinical interpretation of any staining or its absence should be determined by a qualified pathologist and complemented by morphologic studies; controls should be evaluated within the context of the patients clinical history and/or other diagnostic tests.
Principle of method:
BrightVision, one step detection system Goat Anti- Mouse HRP and Rabbit AP (Ready-to-Use).
Reagents provided:
BrightVision, One step detection system Goat anti-Mouse HRP / Goat anti-Rabbit AP (Ready-to-use; 55 ml)
Storage and handling:
2-8°C and in the dark
Procedure:
1. Deparaffinize and rehydrate tissue section (slide/tissue peparing), 2. Wash Aqua dest (Wash; 2x 5 min), 3. If applicable, HIER or digestive enzyme (pre-treatment), 4. Wash buffer (PBS or TBS buffer; 2x 5 min), 5. H2O2 (conc3%) (Tissue preparing; 10 min), 6. Wash buffer (PBS or TBS buffer; 2x 5 min), 7. Primary mouse and/or rabbit antibody (Antibody; 30 min), 8. Wash buffer (PBS or TBS buffer; 2x 5 min), 9 Detection system, polymer Mouse HRP/Rabbit AP, (Labeled polymer; 30 min), 10. Wash buffer (TBS buffer; 2x 5 min), 11. Substrate (DAB; see applicble IFU), 12. Wash aqua dest (Wash; 2x 2 min), 13. Substrate (Fast Red / New Fuchsin; see applicable IFU), 14. Wash aqua dest (Wash; 2x 2 min), 15. Counterstain and coverslip with aqueous mounting medium.
Quality Control:
A positive control, negative control and reagent control are needed and processed in the same way as the unknow specimen slide to interpret staining results.
For in-vitro Diagnostic Use. The BrightVision one step detection system Goat Anti-Mouse/Rabbit IgG AP, is intended for use in immunohistochemistry for the detection of mouse or rabbit antibodies.
The BrightVision detection system Goat Anti- Mouse/Rabbit AP, is a Ready-to-Use system that has been manufactured to give an optimal staining, when using the protocol advised in this IFU. Prior to staining some routine fixed, paraffin-embedding tissue sections should be subjected to pre-treatment (HIER or digestive enzyme). The BrightVision detection system detects Mouse or Rabbit bound to an antigen in tissue sections. This polymer-complex is then visualized with a suitable substrate/chromogen (not provided). Also available in 110 ml, 500 ml and 1000 ml.
Principle of method:
One step detection system goat anti-mouse/rabbit IgG AP
Reagents provided:
One step detection system Goat anti-Mouse/Rabbit AP (Ready-to-use; 55 ml)
Storage and handling:
2-8°C and in the dark
Procedure:
1. Deparaffinize and rehydrate tissue section (slide/tissue peparing), 2. Wash Aqua dest (Wash; 2x 5 min), 3. If applicable, HIER or digestive enzyme (pre-treatment), 4. Wash buffer (PBS or TBS buffer; 2x 5 min), 5. H2O2 (conc3%) (Tissue preparing; 10 min), 6. Wash buffer (PBS or TBS buffer; 2x 5 min), 7. Primary mouse or rabbit antibody (Antibody; 30 min), 8. Wash buffer (PBS or TBS buffer; 2x 5 min), 9. Detection system, polymer Mouse/Rabbit AP, (Labeled polymer; 30 min), 10. Wash buffer (TBS buffer; 2x 5 min), 11. Substrate (Fast Red / New Fuchsin, see applicable IFU), 12. Wash aqua dest (Wash; 2x 2 min), 13. Counterstain and coverslip with aqueous mounting medium.
Quality Control:
A positive control, negative control and reagent control are needed and processed in the same way as the unknow specimen slide to interpret staining results.
For in-vitro Diagnostic Use. The BrightVision one step detection system peroxidase Goat Anti-Mouse/Rabbit IgG HRP, is intended for use in immunohistochemistry for the detection of mouse or rabbit antibodies.
The BrightVision detection system peroxidase Goat Anti- Mouse/Rabbit HRP, is a Ready-to-Use system that has been manufactured to give an optimal staining, when using the protocol advised in this IFU. Prior to staining some routine fixed, paraffin-embedding tissue sections should be subjected to pre-treatment (HIER or digestive enzyme). The BrightVision detection system detects Mouse or Rabbit bound to an antigen in tissue sections. This polymer-complex is then visualized with a suitable substrate/chromogen (not provided). Also available in 110 ml, 500 ml and 1000 ml.
Principle of method:
One step detection system peroxidase goat anti-mouse/rabbit IgG HRP
Reagents provided:
One step detection system Goat anti-Mouse/Rabbit HRP (Ready-to-use; 55 ml)
Storage and handling:
2-8°C and in the dark
Procedure:
1. Deparaffinize and rehydrate tissue section (slide/tissue peparing), 2. Wash Aqua dest (Wash; 2x 5 min), 3. If applicable, HIER or digestive enzyme (pre-treatment), 4. Wash buffer (PBS or TBS buffer; 2x 5 min), 5. H2O2 (conc3%) (Tissue preparing; 10 min), 6. Wash buffer (PBS or TBS buffer; 2x 5 min), 7. Primary mouse or rabbit antibody (Antibody; 30 min), 8. Wash buffer (PBS or TBS buffer; 2x 5 min), 9. Detection system, polymer HRP, (Labeled polymer; 30 min), 10. Wash buffer (PBS or TBS buffer; 2x 5 min), 11. Substrate (DAB; see applicable IFU), 12. Wash aqua dest (Wash; 2x 2 min), 13. Counterstain, dehydrate and coverslip (Auxiliary)
Quality Control:
A positive control, negative control and reagent control are needed and processed in the same way as the unknow specimen slide to interpret staining results.
For in-vitro Diagnostic Use. The BrightVision Ultimate one step component detection system peroxidase Goat Anti-Mouse/Rabbit IgG HRP and DAB, is intended for use in immunohistochemistry for the detection of mouse or rabbit antibodies.
The BrightVision Ultimate detection system, peroxidase Goat Anti-Mouse/Rabbit HRP plus DAB, is a Ready-to-Use system that has been manufactured to give an optimal staining, when using the protocol advised in this IFU. Prior to staining some routine fixed, paraffin-embedding tissue sections should be subjected to pre-treatment (HIER or digestive enzyme). The BrightVision Ultimate detection system detects Mouse or Rabbit bound to an antigen in tissue sections. This polymer-complex is then visualized with a suitable substrate/chromogen. The clinical interpretation of any staining or its absence should be determined by a qualified pathologist and complemented by morphologic studies; controls should be evaluated within the context of the patients clinical history and/or other diagnostic tests.
Principle of method:
BrightVision Ultimate, one step detection system, Goat Anti-Mouse/Rabbit HRP (Ready-to-Use) plus DAB.
Reagents provided:
BrightVision Ultimate plus, one detection system, Goat Anti-Mouse/Rabbit HRP (Ready-to-Use) plus DAB 1. one step detection, Goat Anti-Mouse/Rabbit HRP (Ready-to-Use): 55 ml 2. DAB Solution A: Buffered H2O2 (Ready-to-Use): 83 ml 4. DAB Solution B: Concentrated DAB solution: 4 ml
Storage and handling:
2-8°C and in the dark
Reagent preparation:
Preparation DAB: Add 40?l DAB Solution B (one drop) to 1 ml substrate Solution A, mix well. Volume and the quality of the Bright DAB has been formulized so they also can be used in automatic stainers, when a higher volume is required.
Procedure:
1. Deparaffinize and rehydrate tissue section (slide/tissue peparing), 2. Wash Aqua dest (Wash; 2x 5 min), 3. If applicable, HIER or digestive enzyme (pre-treatment), 4. Wash buffer (PBS or TBS buffer; 2x 5 min), 5. H2O2 (conc3%) (Tissue preparing; 10 min), 6. Wash buffer (PBS or TBS buffer; 2x 5 min), 7. Primary mouse or rabbit antibody (Antibody; 30 min), 8. Wash buffer (PBS or TBS buffer; 2x 5 min), 9 Detection system, polymer Mouse/Rabbit HRP, (Labeled polymer; 30 min), 10. Wash buffer (PBS or TBS buffer; 2x 5 min), 11. Substrate (DAB; 8 min.; Preparation DAB: Add 40?l DAB Solution B (one drop) to 1 ml substrate Solution A, mix well. Volume and the quality of the Bright DAB has been formulized so they also can be used in automatic stainers, when a higher volume is required.), 14. Wash aqua dest (Wash; 2x 2 min), 15. Counterstain, dyhydrate and coverslip (Auxiliary.
Quality Control:
A positive control, negative control and reagent control are needed and processed in the same way as the unknow specimen slide to interpret staining results.
For in-vitro Diagnostic Use. The BrightVision one step detection system Goat Anti-Rabbit IgG AP, is intended for use in immunohistochemistry for the detection of rabbit antibodies.
The BrightVision detection system Goat Anti-Rabbit AP, is a Ready-to-Use system that has been manufactured to give an optimal staining, when using the protocol advised in this IFU. Prior to staining some routine fixed, paraffin-embedding tissue sections should be subjected to pre-treatment (HIER or digestive enzyme). The BrightVision detection system detects Rabbit bound to an antigen in tissue sections. This polymer-complex is then visualized with a suitable substrate/chromogen (not provided). Also available in 110 ml, 500 ml and 1000 ml.
Principle of method:
One step detection system goat anti-rabbit IgG AP
Reagents provided:
One step detection system Goat anti-Rabbit AP (Ready-to-use; 55 ml)
Storage and handling:
2-8°C and in the dark
Procedure:
1. Deparaffinize and rehydrate tissue section (slide/tissue peparing), 2. Wash Aqua dest (Wash; 2x 5 min), 3. If applicable, HIER or digestive enzyme (pre-treatment), 4. Wash buffer (PBS or TBS buffer; 2x 5 min), 5. H2O2 (conc3%) (Tissue preparing; 10 min), 6. Wash buffer (PBS or TBS buffer; 2x 5 min), 7. Primary rabbit antibody (Antibody; 30 min), 8. Wash buffer (TBS buffer; 2x 5 min), 9. Detection system, polymer Rabbit AP, (Labeled polymer; 30 min), 10. Wash buffer (TBS buffer; 2x 5 min), 11. Substrate (Fast Red / New Fuchsin; see applicable IFU), 12. Wash aqua dest (Wash; 2x 2 min), 12. Counterstain and coverslip with aqueous mounting medium.
Quality Control:
A positive control, negative control and reagent control are needed and processed in the same way as the unknow specimen slide to interpret staining results.
For in-vitro Diagnostic Use. The BrightVision one step detection system peroxidase Goat Anti-Rabbit IgG HRP, is intended for use in immunohistochemistry for the detection of rabbit antibodies.
The BrightVision detection system peroxidase Goat Anti-Rabbit HRP, is a Ready-to-Use system that has been manufactured to give an optimal staining, when using the protocol advised in this IFU. Prior to staining some routine fixed, paraffin-embedding tissue sections should be subjected to pre-treatment (HIER or digestive enzyme). The BrightVision detection system detects Rabbit bound to an antigen in tissue sections. This polymer-complex is then visualized with a suitable substrate/chromogen (not provided). Also available in 110 ml, 500 ml and 1000 ml.
Principle of method:
One step detection system peroxidase goat anti-rabbit IgG HRP
Reagents provided:
One step detection system Goat anti-Rabbit HRP (Ready-to-use; 55 ml)
Storage and handling:
2-8°C and in the dark
Procedure:
1. Deparaffinize and rehydrate tissue section (slide/tissue peparing), 2. Wash Aqua dest (Wash; 2x 5 min), 3. If applicable, HIER or digestive enzyme (pre-treatment), 4. H2O2 (conc3%) (Tissue preparing; 10 min), 5. Wash buffer (PBS or TBS buffer; 2x 5 min), 6. Primary rabbit antibody (Antibody; 30 min), 7. Wash buffer (PBS or TBS buffer; 2x 5 min), 8. Detection system, polymer Rabbit HRP, (Labeled polymer; 30 min), 9. Wash buffer (PBS or TBS buffer; 2x 5 min), 10. Substrate (DAB; see applicable IFU), 11. Wash aqua dest (Wash; 2x 2 min), 12. Counterstain, dehydrate and coverslip (Auxiliary)
Quality Control:
A positive control, negative control and reagent control are needed and processed in the same way as the unknow specimen slide to interpret staining results.
For in-vitro Diagnostic Use. The BrightVision one step detection system Goat Anti-Rabbit IgG HRP and Goat anti-Mouse IgG AP, is intended for use in immunohistochemistry for the detection of mouse or rabbit antibodies.
The BrightVision detection system, peroxidase Goat Anti-Rabbit HRP and Goat anti-Mouse AP, is a Ready-to-Use system that has been manufactured to give an optimal staining, when using the protocol advised in this IFU. Prior to staining some routine fixed, paraffin-embedding tissue sections should be subjected to pre-treatment (HIER or digestive enzyme). The BrightVision detection system detects Rabbit and Mouse bound to an antigen in tissue sections. This polymer-complex is then visualized with a suitable substrate/chromogen. The clinical interpretation of any staining or its absence should be determined by a qualified pathologist and complemented by morphologic studies; controls should be evaluated within the context of the patients clinical history and/or other diagnostic tests.
Principle of method:
BrightVision, one step detection system Goat Anti- Rabbit HRP and Mouse AP (Ready-to-Use).
Reagents provided:
BrightVision, One step detection system Goat anti-Rabbit HRP / Goat anti-Mouse AP (Ready-to-use; 55 ml)
Storage and handling:
2-8°C and in the dark
Procedure:
1. Deparaffinize and rehydrate tissue section (slide/tissue peparing), 2. Wash Aqua dest (Wash; 2x 5 min), 3. If applicable, HIER or digestive enzyme (pre-treatment), 4. Wash buffer (PBS or TBS buffer; 2x 5 min), 5. H2O2 (conc3%) (Tissue preparing; 10 min), 6. Wash buffer (PBS or TBS buffer; 2x 5 min), 7. Primary mouse and/or rabbit antibody (Antibody; 30 min), 8. Wash buffer (PBS or TBS buffer; 2x 5 min), 9 Detection system, polymer Rabbit HRP/Mouse AP, (Labeled polymer; 30 min), 10. Wash buffer (TBS buffer; 2x 5 min), 11. Substrate (DAB; see applicble IFU), 12. Wash aqua dest (Wash; 2x 2 min), 13. Substrate (Fast Red / New Fuchsin; see applicable IFU), 14. Wash aqua dest (Wash; 2x 2 min), 15. Counterstain and coverslip with aqueous mounting medium.
Quality Control:
A positive control, negative control and reagent control are needed and processed in the same way as the unknow specimen slide to interpret staining results.
For in-vitro Diagnostic Use. The BrightVision one step detection system peroxidase Goat Anti-Mouse IgG HRP, is intended for use in immunohistochemistry for the detection of mouse antibodies.
The BrightVision detection system peroxidase Goat Anti- Mouse HRP, is a Ready-to-Use system that has been manufactured to give an optimal staining, when using the protocol advised in this IFU. Prior to staining some routine fixed, paraffin-embedding tissue sections should be subjected to pre-treatment (HIER or digestive enzyme). The BrightVision detection system detects Mouse bound to an antigen in tissue sections. This polymer-complex is then visualized with a suitable substrate/chromogen (not provided). Also available in 110 ml, 500 ml and 1000 ml.
Principle of method:
One step detection system peroxidase goat anti-mouse IgG HRP
Reagents provided:
One step detection system Goat anti-Mouse HRP (Ready-to-use; 55 ml)
Storage and handling:
2-8°C and in the dark
Procedure:
1. Deparaffinize and rehydrate tissue section (slide/tissue peparing), 2. Wash Aqua dest (Wash; 2x 5 min), 3. If applicable, HIER or digestive enzyme (pre-treatment), 4. Wash buffer (PBS or TBS buffer; 2x 5 min), 5. H2O2 (conc3%) (Tissue preparing; 10 min), 6. Wash buffer (PBS or TBS buffer; 2x 5 min), 7. Primary mouse antibody (Antibody; 30 min), 8. Wash buffer (PBS or TBS buffer; 2x 5 min), 9. Detection system, step 1, polymer Mouse, (Labeled polymer; 30 min), 10. Wash buffer (PBS or TBS buffer; 2x 5 min), 11. Substrate (DAB; see applicable IFU), 12. Wash aqua dest (Wash; 2x 2 min), 13. Counterstain, dehydrate and coverslip (Auxiliary)
Quality Control:
A positive control, negative control and reagent control are needed and processed in the same way as the unknow specimen slide to interpret staining results.
For in-vitro Diagnostic Use. The BrightVision two components detection system peroxidase Goat Anti-Mouse/Rabbit IgG AP, is intended for use in immunohistochemistry for the detection of mouse or rabbit antibodies.
The BrightVision detection system peroxidase Goat Anti- Mouse/Rabbit AP, is a Ready-to-Use system that has been manufactured to give an optimal staining, when using the protocol advised in this IFU. Prior to staining some routine fixed, paraffin-embedding tissue sections should be subjected to pre-treatment (HIER or digestive enzyme). The BrightVision detection system detects Mouse or Rabbit bound to an antigen in tissue sections. This polymer-complex is then visualized with a suitable substrate/chromogen (not provided).
Principle of method:
Two steps detection system goat anti-mouse/rabbit AP
Reagents provided:
Post-blocking (ready-to-use) 55 ml and Polymer Goat Anti-Mouse/Rabbit AP (ready-to-use) 55 ml.
Storage and handling:
2-8°C and in the dark
Procedure:
1. Deparaffinize and rehydrate tissue section (slide/tissue peparing), 2. Wash Aqua dest (Wash; 2x 5 min), 3. If applicable, HIER or digestive enzyme (pre-treatment), 4. Wash buffer (PBS or TBS buffer; 2x 5 min), 5. H2O2 (conc3%) (Tissue preparing; 10 min), 6. Wash buffer (PBS or TBS buffer; 2x 5 min), 7. Primary mouse or rabbit antibody (Antibody; 30 min), 8. Wash buffer (PBS or TBS buffer; 2x 5 min), 9. Detection system, step 1, post-blocking (Post-blocking; 15 min), 10. Wash buffer (PBS or TBS buffer; 2x 5 min), 11. Detection system, step 2, polymer Mouse/Rabbit AP (Labeled polymer; 30 min), 12. Wash buffer (PBS or TBS buffer; 2x 5 min), 13. If applicable Substrate (DAB), 14. Wash aqua dest (Wash; 2x 2 min), 15. Counterstain, dehydrate and coverslip (Auxiliary)
Quality Control:
A positive control, negative control and reagent control are needed and processed in the same way as the unknow specimen slide to interpret staining results.
For in-vitro Diagnostic Use. The BrightVision two components detection system peroxidase Goat Anti-Mouse/Rabbit IgG HRP, is intended for use in immunohistochemistry for the detection of mouse or rabbit antibodies.
The BrightVision detection system peroxidase Goat Anti- Mouse/Rabbit HRP, is a Ready-to-Use system that has been manufactured to give an optimal staining, when using the protocol advised in this IFU. Prior to staining some routine fixed, paraffin-embedding tissue sections should be subjected to pre-treatment (HIER or digestive enzyme). The BrightVision detection system detects Mouse or Rabbit bound to an antigen in tissue sections. This polymer-complex is then visualized with a suitable substrate/chromogen (not provided).
Principle of method:
Two steps detection system goat anti-mouse/rabbit HRP
Reagents provided:
Post-blocking (ready-to-use) 55 ml and Polymer Goat Anti-Mouse/Rabbit HRP (ready-to-use) 55 ml.
Storage and handling:
2-8°C and in the dark
Procedure:
1. Deparaffinize and rehydrate tissue section (slide/tissue peparing), 2. Wash Aqua dest (Wash; 2x 5 min), 3. If applicable, HIER or digestive enzyme (pre-treatment), 4. Wash buffer (PBS or TBS buffer; 2x 5 min), 5. H2O2 (conc3%) (Tissue preparing; 10 min), 6. Wash buffer (PBS or TBS buffer; 2x 5 min), 7. Primary mouse or rabbit antibody (Antibody; 30 min), 8. Wash buffer (PBS or TBS buffer; 2x 5 min), 9. Detection system, step 1, post-blocking (Post-blocking; 15 min), 10. Wash buffer (PBS or TBS buffer; 2x 5 min), 11. Detection system, step 2, polymer Mouse/Rabbit HRP (Labeled polymer; 30 min), 12. Wash buffer (PBS or TBS buffer; 2x 5 min), 13. If applicable Substrate (DAB), 14. Wash aqua dest (Wash; 2x 2 min), 15. Counterstain, dehydrate and coverslip (Auxiliary)
Quality Control:
A positive control, negative control and reagent control are needed and processed in the same way as the unknow specimen slide to interpret staining results.
For in-vitro Diagnostic Use. The BrightVision Ultimate plus two steps component detection system peroxidase Goat Anti-Mouse/Rabbit IgG HRP and DAB, is intended for use in immunohistochemistry for the detection of mouse or rabbit antibodies.
The BrightVision Ultimate plus detection system, peroxidase Goat Anti-Mouse/Rabbit HRP plus DAB, is a Ready-to-Use system that has been manufactured to give an optimal staining, when using the protocol advised in this IFU. Prior to staining some routine fixed, paraffin-embedding tissue sections should be subjected to pre-treatment (HIER or digestive enzyme). The BrightVision Ultimate plus detection system detects Mouse or Rabbit bound to an antigen in tissue sections. This polymer-complex is then visualized with a suitable substrate/chromogen. The clinical interpretation of any staining or its absence should be determined by a qualified pathologist and complemented by morphologic studies; controls should be evaluated within the context of the patients clinical history and/or other diagnostic tests.
Principle of method:
BrightVision Ultimate plus, two steps detection system, Goat Anti-Mouse/Rabbit HRP (Ready-to-Use) plus DAB.
Reagents provided:
BrightVision Ultimate plus, two steps detection system, Goat Anti-Mouse/Rabbit HRP (Ready-to-Use) plus DAB 1. Post-blocking (ready-to-use): 55 ml 2. Polymer Goat Anti-Mouse/Rabbit HRP (Ready-to-Use): 55 ml 3. DAB Solution A: Buffered H2O2 (Ready-to-Use): 83 ml 4. DAB Solution B: Concentrated DAB solution: 4 ml
Storage and handling:
2-8°C and in the dark
Reagent preparation:
Preparation DAB: Add 40?l DAB Solution B (one drop) to 1 ml substrate Solution A, mix well. Volume and the quality of the Bright DAB has been formulized so they also can be used in automatic stainers, when a higher volume is required.
Procedure:
1. Deparaffinize and rehydrate tissue section (slide/tissue peparing), 2. Wash Aqua dest (Wash; 2x 5 min), 3. If applicable, HIER or digestive enzyme (pre-treatment), 4. Wash buffer (PBS or TBS buffer; 2x 5 min), 5. H2O2 (conc3%) (Tissue preparing; 10 min), 6. Wash buffer (PBS or TBS buffer; 2x 5 min), 7. Primary mouse or rabbit antibody (Antibody; 30 min), 8. Wash buffer (PBS or TBS buffer; 2x 5 min), 9. Detection system, step 1, post-blocking (Post-blocking; 15 min), 10 Wash buffer (PBS or TBS buffer; 2x 5 in). 11 Detection system, step 2, polymer Mouse/Rabbit HRP, (Labeled polymer; 30 min), 12. Wash buffer (PBS or TBS buffer; 2x 5 min), 13. Substrate (DAB; 8 min; Preparation DAB: Add 40?l DAB Solution B (one drop) to 1 ml substrate Solution A, mix well. Volume and the quality of the Bright DAB has been formulized so they also can be used in automatic stainers, when a higher volume is required.), 14. Wash aqua dest (Wash; 2x 2 min), 15. Counterstain, dyhydrate and coverslip (Auxiliary.
Quality Control:
A positive control, negative control and reagent control are needed and processed in the same way as the unknow specimen slide to interpret staining results.
For in-vitro Diagnostic Use. The BrightVision two components detection system peroxidase Goat Anti-Mouse/Rabbit IgG AP, is intended for use in immunohistochemistry for the detection of mouse or rabbit antibodies.
The BrightVision detection system peroxidase Goat Anti- Mouse/Rabbit AP, is a Ready-to-Use system that has been manufactured to give an optimal staining, when using the protocol advised in this IFU. Prior to staining some routine fixed, paraffin-embedding tissue sections should be subjected to pre-treatment (HIER or digestive enzyme). The BrightVision detection system detects Mouse or Rabbit bound to an antigen in tissue sections. This polymer-complex is then visualized with a suitable substrate/chromogen (not provided).
Principle of method:
Two steps detection system goat anti-mouse/rabbit AP
Reagents provided:
Post-blocking (ready-to-use) (Gold) 55 ml and Polymer Goat Anti-Mouse/Rabbit AP (ready-to-use) (Ruby) 55 ml.
Storage and handling:
2-8°C and in the dark
Procedure:
1. Deparaffinize and rehydrate tissue section (slide/tissue peparing), 2. Wash Aqua dest (Wash; 2x 5 min), 3. If applicable, HIER or digestive enzyme (pre-treatment), 4. Wash buffer (PBS or TBS buffer; 2x 5 min), 5. H2O2 (conc3%) (Tissue preparing; 10 min), 6. Wash buffer (PBS or TBS buffer; 2x 5 min), 7. Primary mouse or rabbit antibody (Antibody; 30 min), 8. Wash buffer (PBS or TBS buffer; 2x 5 min), 9. Detection system, step 1, post-blocking (Post-blocking; 15 min), 10. Wash buffer (PBS or TBS buffer; 2x 5 min), 11. Detection system, step 2, polymer Mouse/Rabbit AP (Labeled polymer; 30 min), 12. Wash buffer (PBS or TBS buffer; 2x 5 min), 13. If applicable Substrate (DAB), 14. Wash aqua dest (Wash; 2x 2 min), 15. Counterstain, dehydrate and coverslip (Auxiliary)
Quality Control:
A positive control, negative control and reagent control are needed and processed in the same way as the unknow specimen slide to interpret staining results.
For in-vitro Diagnostic Use. The BrightVision two components detection system peroxidase Goat Anti-Mouse/Rabbit IgG HRP, is intended for use in immunohistochemistry for the detection of mouse or rabbit antibodies.
The BrightVision detection system peroxidase Goat Anti- Mouse/Rabbit HRP, is a Ready-to-Use system that has been manufactured to give an optimal staining, when using the protocol advised in this IFU. Prior to staining some routine fixed, paraffin-embedding tissue sections should be subjected to pre-treatment (HIER or digestive enzyme). The BrightVision detection system detects Mouse or Rabbit bound to an antigen in tissue sections. This polymer-complex is then visualized with a suitable substrate/chromogen (not provided).
Principle of method:
Two steps detection system goat anti-mouse/rabbit HRP
Reagents provided:
Post-blocking (ready-to-use) (Gold) 55 ml and Polymer Goat Anti-Mouse/Rabbit HRP (ready-to-use) (Ruby) 55 ml.
Storage and handling:
2-8°C and in the dark
Procedure:
1. Deparaffinize and rehydrate tissue section (slide/tissue peparing), 2. Wash Aqua dest (Wash; 2x 5 min), 3. If applicable, HIER or digestive enzyme (pre-treatment), 4. Wash buffer (PBS or TBS buffer; 2x 5 min), 5. H2O2 (conc3%) (Tissue preparing; 10 min), 6. Wash buffer (PBS or TBS buffer; 2x 5 min), 7. Primary mouse or rabbit antibody (Antibody; 30 min), 8. Wash buffer (PBS or TBS buffer; 2x 5 min), 9. Detection system, step 1, post-blocking (Post-blocking; 15 min), 10. Wash buffer (PBS or TBS buffer; 2x 5 min), 11. Detection system, step 2, polymer Mouse/Rabbit HRP (Labeled polymer; 30 min), 12. Wash buffer (PBS or TBS buffer; 2x 5 min), 13. If applicable Substrate (DAB), 14. Wash aqua dest (Wash; 2x 2 min), 15. Counterstain, dehydrate and coverslip (Auxiliary)
Quality Control:
A positive control, negative control and reagent control are needed and processed in the same way as the unknow specimen slide to interpret staining results.
s Broad Spectrum Protease Inhibitor Cocktail (EDTA free) is a complex of various protease inhibitors, which has been tested for inhibiting proteases and esterase broadly.
Product Type:
Chemicals & Biochemicals
Storage Temp:
-20°C
Additional Info:
Bosterâs Broad Spectrum Protease Inhibitor Cocktail (EDTA free) is a complex of various protease inhibitors, which has been tested for inhibiting proteases and esterase broadly.Â
Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Ananas comosus
Expected Species:
Ananas comosus
Immunogen:
native bromelain isolated and purified from Ananas comosus
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography
Application Details:
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG in PBS.
Reconstitution:
For reconstitution add 1 ml of sterile destilled water
Molecular Weight:
39 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Ananas comosus
Expected Species:
Ananas comosus
Immunogen:
native bromelain isolated and purified from Ananas comosus
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran
Application Details:
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Purity:
Immunogen affinity purified IgG in PBS.
Reconstitution:
For reconstitution add 0,5 ml of sterile destilled water
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Ananas comosus
Expected Species:
Ananas comosus
Immunogen:
native bromelain isolated and purified from Ananas comosus
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Biotin/IgG protein molar ration is approximately 6,2, No foreign proteins are added
Application Details:
1 : 5000-1 : 50 000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG in PBS.
Reconstitution:
For reconstitution add 1 ml of sterile destilled water
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is labelled with biotin using N-hydroxysuccinimidobiotin, Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Bile Salt Export Pump (BSEP) is a member of the ATP-binding cassette (ABC) transporters, and mediates the transport of bile acid, taurocholate, and other cholate conjugates across the hepatocyte canalicular membrane into the canaliculus. BSEP is associated with progressive familial intrahepatic cholestasis type 2 (PFIC2) and benign recurrent intrahepatic cholestasis type 2 (BRIC2). PFIC2 caused by mutations in the BSEP gene increases the risk of hepatocellular carcinoma in early life.
Bile Salt Export Pump (BSEP) is a member of the ATP-binding cassette (ABC) transporters, and mediates the transport of bile acid, taurocholate, and other cholate conjugates across the hepatocyte canalicular membrane into the canaliculus. BSEP is associated with progressive familial intrahepatic cholestasis type 2 (PFIC2) and benign recurrent intrahepatic cholestasis type 2 (BRIC2). PFIC2 caused by mutations in the BSEP gene increases the risk of hepatocellular carcinoma in early life.
Bile Salt Export Pump (BSEP) is a member of the ATP-binding cassette (ABC) transporters, and mediates the transport of bile acid, taurocholate, and other cholate conjugates across the hepatocyte canalicular membrane into the canaliculus. BSEP is associated with progressive familial intrahepatic cholestasis type 2 (PFIC2) and benign recurrent intrahepatic cholestasis type 2 (BRIC2). PFIC2 caused by mutations in the BSEP gene increases the risk of hepatocellular carcinoma in early life.
Affinity purified using solid phase human C1 inhibitor. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0).Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative. Antibody purity is > 95% based on SDS-PAGE.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen Affinity purified using solid phase human C1 Inhibitor.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
The C4d [IHC519] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
The C4d [IHC519] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
The C4d [IHC519] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
CA-125 is normally found in epithelial cells of Fallopian tube, endometrium and endocervix, pancreas, colon, gall bladder, stomach, kidney, apocrine sweat gland, mammary gland, and mesothelial cell lining of pleura, pericardium, and the peritoneum. Anti-CA-125 reacts positively with ovarian malignancies, cervical carcinoma, seminal vesicle carcinoma, anaplastic lymphoma, and endometrial and bladder adenocarcinoma.
CA-125 is normally found in epithelial cells of Fallopian tube, endometrium and endocervix, pancreas, colon, gall bladder, stomach, kidney, apocrine sweat gland, mammary gland, and mesothelial cell lining of pleura, pericardium, and the peritoneum. Anti-CA-125 reacts positively with ovarian malignancies, cervical carcinoma, seminal vesicle carcinoma, anaplastic lymphoma, and endometrial and bladder adenocarcinoma.
CA-125 is normally found in epithelial cells of Fallopian tube, endometrium and endocervix, pancreas, colon, gall bladder, stomach, kidney, apocrine sweat gland, mammary gland, and mesothelial cell lining of pleura, pericardium, and the peritoneum. Anti-CA-125 reacts positively with ovarian malignancies, cervical carcinoma, seminal vesicle carcinoma, anaplastic lymphoma, and endometrial and bladder adenocarcinoma.
CA 19-9 is a secreted protein that is implicated in various cancers. It is overexpressed in salivary gland mucoepidermoid carcinomas and gastric, pancreatic, and colonic (gastrointestinal) adenocarcinomas, but is not expressed in breast, kidney, and prostate carcinomas. CA 19-9 staining is also implicated in Mirizzi’s Syndrome or other bile duct and liver diseases.
CA 19-9 is a secreted protein that is implicated in various cancers. It is overexpressed in salivary gland mucoepidermoid carcinomas and gastric, pancreatic, and colonic (gastrointestinal) adenocarcinomas, but is not expressed in breast, kidney, and prostate carcinomas. CA 19-9 staining is also implicated in Mirizzi’s Syndrome or other bile duct and liver diseases.
CA 19-9 is a secreted protein that is implicated in various cancers. It is overexpressed in salivary gland mucoepidermoid carcinomas and gastric, pancreatic, and colonic (gastrointestinal) adenocarcinomas, but is not expressed in breast, kidney, and prostate carcinomas. CA 19-9 staining is also implicated in Mirizzi’s Syndrome or other bile duct and liver diseases.
The Cadherin-17 [IHC520] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist
The Cadherin-17 [IHC520] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist
The Cadherin-17 [IHC520] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissue within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist
CAH3 - carbonic anhydrase is a zinc-containing metalloenzyme that catalyzes the reversible interconversion of CO2 and HCO3?. It plays an important role in many physiological functions that involve decarboxylation or carboxylation reactions, including both photosynthesis and respiration.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Chlamydomonas reinhardtii (only)
Immunogen:
Recombinant carbonic anhydrase of Chlamydomonas reinhardtii A8J4Z8 after cleavage of a fusion protein
Applications:
immunofluorescence (IF), Immunogold (IG), Western blot (WB)
Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022). https://doi.org/10.1038/s41586-022-04662-9Shukshina &Terentyev (2021) Involvement of Carbonic Anhydrase CAH3 in the Structural and Functional Stabilization of the Water-Oxidizing Complex of Photosystem II from Chlamydomonas reinhardtii. Biochemistry Moscow 86, 867–877 (2021). https://doi.org/10.1134/S0006297921070075Terentyev (2020: The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated From Wild Type and cia3 Mutant Chlamydomonas reinhardtii. Life (Basel). 2020 May 14;10(5):E63. doi: 10.3390/life10050063.Terentyev et al. (2019). Carbonic anhydrase CAH3 supports the activity of photosystem II under increased pH. Biochim Biophys Acta Bioenerg. 2019 Jul 1;1860(7):582-590. doi: 10.1016/j.bbabio.2019.06.003.Correa-Galvis et al. (2016). Photosystem II Subunit PsbS Is Involved in the Induction of LHCSR Protein-dependent Energy Dissipation in Chlamydomonas reinhardtii. J Biol Chem. 2016 Aug 12;291(33):17478-87. doi: 10.1074/jbc.M116.737312.
Special application note:
This antibody recognizes native and recombinant carbonic anhydrase of Chlamydomonas reinhardtii.
CAH6 (Carbonic anhydrase 6) is a zinc-containing metalloenzymes that catalyze the reversible hydration of CO2. There are three evolutionary not related families of carbonic anhydrases: alpha, beta and gamma. CAH6 belonds to the beta family and initially was localized to chloroplasts Mitra et al. (2004).However, lately localization to flagella has been confirmed Mackinder et al. 2017.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Gonium pectorale, Volvox carteriSpecies of your interest not listed? Contact us
Immunogen:
Recombinant, full length CAH6 of Chlamydomonas reinhardtii, excised from the gel, UniProt: Q6S7R9
Lately localization to flagella has been confirmed for CAH6 Mackinder et al. 2017.Extraction method – harvest the cells and add SDS sample buffer.
Mackinder et al. 2017. A Spatial Interactome Reveals the Protein Organization of the Algal CO2-Concentrating Mechanism. Cell. 2017 Sep 21;171(1):133-147.e14. doi: 10.1016/j.cell.2017.08.044.Mitra et al. (2004). Identification of a new chloroplast carbonic anhydrase in Chlamydomonas reinhardtii. Plant Physiol. 2004 May;135(1):173-82.
Mouse anti-Calbindin-binding protein Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Buffers cytosolic calcium. May stimulate a membrane Ca<sup>2+</sup>-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Chicken,Horse,Human,Mouse,Pig,Rat
Immunogen:
Full-length recombinant human protein
Applications:
IHC-Frozen,WB
Clone number:
4H7
Antibody Isotype:
IgG1
Application Details:
Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human, reacts with Human, horse, Cow, Pig, chicken, Rat, Mouse.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Chicken anti-Calbindin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Buffers cytosolic calcium. May stimulate a membrane Ca<sup>2+</sup>-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Bovine,Human,Mouse,Rat
Immunogen:
Full-length recombinant human protein
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human, reacts with human, cow, rat, mouse. Antibody is specific for calbindin and does not recognize closely related proteins parvalbumin and calretinin as determined by Western Blotting.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
The Calbindin D-28K Antibody was raised to Calbindin D-28k purified from bovine cerebellum. The antibody has a proven maximum biotin-streptavidin/HRP staining at a 1/5,000 - 1/10,000 dilution and a 4+ indirect immunofluorescence staining at a 1/500 - 1/1,000 dilution in rat striatum, cortex, and hippocampus. The antiserum has been characterized as specific to calbindin D-28k; please see reference listed below. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.
Calcineurine is a calcium-binding protein involved in signal transduction.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Hordeum vulgare, Populus sp., Triticum aestivum
Expected Species:
Brassica napus, Theobroma cacao Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known calcineurin/EF-hand motif sequences including Arabidopsis thaliana CPK21, UniProt: Q9ZSA2, TAIR: AT4G04720 and fpound in other calcium-dependent protein kinases
Calcitonin is a polypeptide hormone formed by the proteolytic cleavage of a larger prepropeptide. It is produced primarily by the parafollicular C-cells of the thyroid, and is involved in the regulation of calcium and phosphorus metabolism. It decreases the level of calcium and phosphate ions in blood by promoting the incorporation of these ions into bones, as well as inhibiting renal tubular cell reabsorption. Calcitonin expression is found in C-cell hyperplasia and medullary thyroid carcinomas. It is a useful marker in the identification of C-cell proliferative abnormalities, and for distinguishing medullary carcinoma from papillary and follicular thyroid cancer.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC521
Antibody Isotype:
IgG
GMDN Code:
56870
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Thyroid, Thyroid Medullary Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Calcitonin is a polypeptide hormone formed by the proteolytic cleavage of a larger prepropeptide. It is produced primarily by the parafollicular C-cells of the thyroid, and is involved in the regulation of calcium and phosphorus metabolism. It decreases the level of calcium and phosphate ions in blood by promoting the incorporation of these ions into bones, as well as inhibiting renal tubular cell reabsorption. Calcitonin expression is found in C-cell hyperplasia and medullary thyroid carcinomas. It is a useful marker in the identification of C-cell proliferative abnormalities, and for distinguishing medullary carcinoma from papillary and follicular thyroid cancer.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC521
Antibody Isotype:
IgG
GMDN Code:
56870
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Thyroid, Thyroid Medullary Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Calcitonin is a polypeptide hormone formed by the proteolytic cleavage of a larger prepropeptide. It is produced primarily by the parafollicular C-cells of the thyroid, and is involved in the regulation of calcium and phosphorus metabolism. It decreases the level of calcium and phosphate ions in blood by promoting the incorporation of these ions into bones, as well as inhibiting renal tubular cell reabsorption. Calcitonin expression is found in C-cell hyperplasia and medullary thyroid carcinomas. It is a useful marker in the identification of C-cell proliferative abnormalities, and for distinguishing medullary carcinoma from papillary and follicular thyroid cancer.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC521
Antibody Isotype:
IgG
GMDN Code:
56870
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Thyroid, Thyroid Medullary Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Caldesmon is a marker for smooth muscle differentiation. Found in smooth muscle and other tissues, caldesmon interacts with Ca<sup>2+</sup>-calmodulin, actin, tropomyosin, myosin, and phospholipids. It inhibits the ATPase activity of myosin in smooth muscle, and mediates Ca<sup>2+</sup>-dependent inhibition of smooth muscle and non-muscle contraction. Caldesmon expression is found in Gastrointestinal Stromal Tumours (GIST), and can be used to differentiate epithelioid mesothelioma from serous papillary carcinoma of the ovary. It is also a specific marker for smooth muscle cells (SMC) and associated neoplasms; therefore, Anti-Caldesmon can be used in the study of the SMC differentiation process as well as the differentiation of other tumours with SMC-like differentiation, including leiomyosarcoma and myofibroblastic tumours.
Caldesmon is a marker for smooth muscle differentiation. Found in smooth muscle and other tissues, caldesmon interacts with Ca<sup>2+</sup>-calmodulin, actin, tropomyosin, myosin, and phospholipids. It inhibits the ATPase activity of myosin in smooth muscle, and mediates Ca<sup>2+</sup>-dependent inhibition of smooth muscle and non-muscle contraction. Caldesmon expression is found in Gastrointestinal Stromal Tumours (GIST), and can be used to differentiate epithelioid mesothelioma from serous papillary carcinoma of the ovary. It is also a specific marker for smooth muscle cells (SMC) and associated neoplasms; therefore, Anti-Caldesmon can be used in the study of the SMC differentiation process as well as the differentiation of other tumours with SMC-like differentiation, including leiomyosarcoma and myofibroblastic tumours.
Caldesmon is a marker for smooth muscle differentiation. Found in smooth muscle and other tissues, caldesmon interacts with Ca<sup>2+</sup>-calmodulin, actin, tropomyosin, myosin, and phospholipids. It inhibits the ATPase activity of myosin in smooth muscle, and mediates Ca<sup>2+</sup>-dependent inhibition of smooth muscle and non-muscle contraction. Caldesmon expression is found in Gastrointestinal Stromal Tumours (GIST), and can be used to differentiate epithelioid mesothelioma from serous papillary carcinoma of the ovary. It is also a specific marker for smooth muscle cells (SMC) and associated neoplasms; therefore, Anti-Caldesmon can be used in the study of the SMC differentiation process as well as the differentiation of other tumours with SMC-like differentiation, including leiomyosarcoma and myofibroblastic tumours.
Calponin is an actin, tropomyosin and calmodulin-binding protein that involves in the regulation of smooth muscle contraction. This antibody is mainly used for the diagnosis and research of myoepithelial cells in leiomyoma and breast lesions.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC075
Antibody Isotype:
IgG
GMDN Code:
56877
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Thyroid, Thyroid Medullary Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Calponin is an actin, tropomyosin and calmodulin-binding protein that involves in the regulation of smooth muscle contraction. This antibody is mainly used for the diagnosis and research of myoepithelial cells in leiomyoma and breast lesions.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC075
Antibody Isotype:
IgG
GMDN Code:
56877
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Thyroid, Thyroid Medullary Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Calponin is an actin, tropomyosin and calmodulin-binding protein that involves in the regulation of smooth muscle contraction. This antibody is mainly used for the diagnosis and research of myoepithelial cells in leiomyoma and breast lesions.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC075
Antibody Isotype:
IgG
GMDN Code:
56877
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Thyroid, Thyroid Medullary Carcinoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The Calretinin Antibody was raised to chick calretinin fusion protein. The antibody has a proven maximum biotin-avidin/HRP staining at a 1/2000 - 1/4000 dilution in rat cortex, hippocampus and hypothalamus.
Mouse anti-Calretinin-binding protein (CR) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
Calretinin is a calcium-binding protein which is abundant in auditory neurons. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Rat
Immunogen:
Full-length recombinant human protein
Applications:
IHC-Frozen,WB
Clone number:
3G9
Antibody Isotype:
IgG1
Application Details:
Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CR, 29 kDa calbindin
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human, reacts with Human, Cow, Rat, Mouse. Antibody is specific for calbindin and does not recognize closely related proteins parvalbumin, calretinin and secretagogin as determined by Western Blotting.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Calretinin is a calcium-binding protein that functions as a modulator of neuronal excitability and may play a protective role in the survival of nerve cells during disturbances in calcium homeostasis. It is abundantly expressed in subsets of neurons throughout the brain and spinal cord, particularly retina and sensory ganglia, but it is also found in mesothelium, eccrine sweat glands, Sertoli cells, ovarian stromal cells, and adrenal cortical cells. Due to its high sensitivity against mesothelial cells, calretinin is a useful marker in differentiating mesothelioma and metastatic adenocarcinoma to the serous membranes. It is also a diagnostic marker of Hirschsprung's disease and some ovarian and testicular cancers such as Sertoli-Leydig cell tumour, Sertoli cell tumour, Leydig cell tumour, sex cord tumour with annular tubules, and steroid cell tumour.
Calretinin is a calcium-binding protein that functions as a modulator of neuronal excitability and may play a protective role in the survival of nerve cells during disturbances in calcium homeostasis. It is abundantly expressed in subsets of neurons throughout the brain and spinal cord, particularly retina and sensory ganglia, but it is also found in mesothelium, eccrine sweat glands, Sertoli cells, ovarian stromal cells, and adrenal cortical cells. Due to its high sensitivity against mesothelial cells, calretinin is a useful marker in differentiating mesothelioma and metastatic adenocarcinoma to the serous membranes. It is also a diagnostic marker of Hirschsprung's disease and some ovarian and testicular cancers such as Sertoli-Leydig cell tumour, Sertoli cell tumour, Leydig cell tumour, sex cord tumour with annular tubules, and steroid cell tumour.
Chicken anti-Calretinin (CR) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Calretinin is a calcium-binding protein which is abundant in auditory neurons. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Bovine,Human,Mouse,Rat
Immunogen:
Full-length recombinant human protein
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CR; 29 kDa calbindin;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human, reacts with human, cow, rat, mouse. Antibody is specific for calretinin and does not recognize closely related proteins parvalbumin and calbindin as determined by Western Blotting.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Calretinin is a calcium-binding protein which is abundant in auditory neurons. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Bovine,Human,Mouse,Rat
Immunogen:
Full-length recombinant human protein
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
Western blotting (1:5,000-1:10,000), Immunohistochemistry (1:5,000-1:10,000) and Immunocytochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CR, 29 kDa calbindin
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human,reacts with Human, Cow, Rat, Mouse. Antibody is specific for calretinin and does not recognize closely related proteins parvalbumin and calbindin as determined by Western Blotting.
Storage:
Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Calretinin is a calcium-binding protein that functions as a modulator of neuronal excitability and may play a protective role in the survival of nerve cells during disturbances in calcium homeostasis. It is abundantly expressed in subsets of neurons throughout the brain and spinal cord, particularly retina and sensory ganglia, but it is also found in mesothelium, eccrine sweat glands, Sertoli cells, ovarian stromal cells, and adrenal cortical cells. Due to its high sensitivity against mesothelial cells, calretinin is a useful marker in differentiating mesothelioma and metastatic adenocarcinoma to the serous membranes. It is also a diagnostic marker of Hirschsprung's disease and some ovarian and testicular cancers such as Sertoli-Leydig cell tumour, Sertoli cell tumour, Leydig cell tumour, sex cord tumour with annular tubules, and steroid cell tumour.
CALS12/PMR4 (Callose synthase 12) is involved in sporophytic and gametophytic development and required for normal leaf development and callose formation induced by wounding and pathogen attack. Alternative names: 1,3-beta-glucan synthase, Protein GLUCAN SYNTHASE-LIKE 5, Protein POWDERY MILDEW RESISTANT 4.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Capsella rubella, Camelina sativa, Eutrema salsugineum, Brassica napus, Brassica oleracea, Brassica rapa, Tarenaya hasslerianaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana CALS12 protein sequence, UniProt: Q9ZT82 , TAIR: At4g03550
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Country Of Origin:
Normal Dog Serum was obtained from healthy animals of Chinese origin.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Rabbit anti-Capsaicin receptor (TrpV1) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
TISSUE SPECIFICITY: Predominantly expressed in trigeminal and dorsal root sensory ganglia. Isoform 1 and isoform 3 are also expressed in brain and peripheral blood mononuclear cells.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse (Predicted),Rat
Immunogen:
A synthetic peptide (YFSHLKEYVAS) of human capsaicin receptor protein (aa: 531-541) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. Use at 1:1000 to 1:2000 dilution. This antibody has not been tested in other applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rehman R et al (2013) TRPV1 inhibition attenuates IL-13 mediated asthma features in mice by reducing airway epithelial injury. Int Immunopharmacol. 2013 Mar;15(3):597-605.
Specificity:
Immunohistochemical analysis of rat DRG indicates a superb degree of specificity for this serum. This antibody is known to react with rat Capsaicin and due to sequence homology is expected to react with mouse Capsaicin . Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent.
Rabbit anti-Capsaicin receptor (TrpV1) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Responses evoked by low pH and heat, and capsaicin can be antagonized by capsazepine. SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (PSESTSHRWRGPA) of human capsaicin receptor protein (aa: 608-621) conjugated to KLH has been used as the antigen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. Use at 1:1000 to 1:2000 dilution. This antibody has not been tested in other applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Jabin Fagelskiold A et al (2012) Insulin-secreting INS-1E cells express functional TRPV1 channels. Islets. 2012 Jan 1;4(1).
Specificity:
Specificity was confirmed by IHC using frozen sections of rat dorsal root ganglia (DRG) and spinal cord. Due to sequence homology, similar staining is predicted in human DRG and spinal cord. Human, rat. Other species have not yet been tested.
Storage:
After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Mouse anti-Capsaicin receptor (TrpV1) Monoclonal Antibody (Unconjugated), suitable for WB, FC.
Background Info:
The capsaicin receptor (VR1, TRPV1) is a ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. The receptor seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. It is involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca2+-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH less than 6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis (Ref: uniprot.org).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS, pH 7.4 with 3% trehalose.
Host Animal:
Mouse
Species Reactivity:
Guinea Pig (Predicted),Mouse,Rat
Immunogen:
A synthetic peptide (C-GSLKPEDAEVFKDSMVPGEK) as a part of the C-terminal rat VR1 protein (aa: 819-838) has been used as the immunogen.
Applications:
FC,WB
Clone number:
BS397
Antibody Isotype:
IgG2b, kappa
Application Details:
Flow Cytometry: 2 ug/10^6 cells. <br><br>Western blotting: 0.5-2 µg/mL , SDS-PAGE on Bis-Tris gel 4-12%, 5% beta-mercaptoethanol, primary antibody O/N incubation in 5% skim milk/TBST. Secondary is anti-mouse-HRP, 1/6000 dilution, 2h at room temperature. Blot developed on Li-Cor? C-DiGit? lot Scanner. <br><br>IHC: Frozen or PEG embedded tissues tested (PEG embedding, see Klosen P et al (1993) J Histochem Cytochem. 41(3):455-63). Conditions tested: 1-10 µg/mL in PBS, 48 hours, followed by detection via directly conjugated fluorescent anti-mouse secondary. Antibody not yet tested on paraffin embedded sections. Other immunohistochemistry methods not yet tested but are expected to be reactive. <br><br>ICC: 4% formaldehyde fixed cells tested; requires permeabilization step as antigen epitope is intracellular. Suggested primary antibody concentration: 1-2 µg/mL.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Matusica D et al. (2020) Differentiation of the 50B11 dorsal root ganglion cells into NGF and GDNF responsive nociceptor subtypes. Mol Pain. 16:1744806920970368 Application: Rat, WB. Bai J et al. (2018) [EXPRESS] Attenuation of TRPV1 by AMG-517 after Nerve Injury Promotes Peripheral Axonal Regeneration in Rats. Mol Pain. [Epub ahead of print] Application: Rat, WB.
Specificity:
Antibody is specific for rat/mouse VR1 protein in westerns and immunofluorescent immunohistochemistry on mouse PEG fixed DRG tissues. Pre-absorption with immunogen obliterates positive staining. Cross reactivity with other non-VR1 proteins is minimal; cross reactivity with VR1 from other species not yet tested. This antibody clone is known to react with rat and mouse TrpV1. It is predicted to react with guinea pig due to sequence homology.
Storage:
Store lyophilized antibody at 2-8ºC. After reconstitution divide in to aliquots and store at -20°C for a higher stability. Antibody contains no preservatives. Storage at 2-8°C with an appropriate antibacterial agent. USE Sterile methods. Highest purity Glycerol (1:1) may be added for an additional stability when stored at refrigerated or freezing temperatures. Avoid repetitive freeze/thaw cycles.
Ascorbate peroxidase (APX) is the enzyme catalyzing the ascorbate-dependent reduction of hydrogen peroxide. Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in fruits and photosynthetic tissues. AA is found in every compartment of the plant cell including the apoplast.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
B gue et al. (2019). CDC48 regulates ascorbate peroxidase in tobacco. J Exp Bot. 2019 Mar 1. pii: erz097. doi: 10.1093/jxb/erz097.Wang et al. (2018). Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity. Proc Natl Acad Sci U S A. 2018 Nov 16. pii: 201813058. doi: 10.1073/pnas.1813058115. Bal žov et al. (2018). Zinc oxide nanoparticles phytotoxicity on halophyte from genus Salicornia. Plant Physiol Biochem. 2018 Sep;130:30-42. doi: 10.1016/j.plaphy.2018.06.013.Adhikari et al. (2018). Sulfate improves cadmium tolerance by limiting cadmium accumulation, modulation of sulfur metabolism and antioxidant defense system in maize. Environmental and Experimental Botany Volume 153, September 2018, Pages 143-162.Ferrer et al. (2018). Differential Pb tolerance in metallicolous and non-metallicolous Zygophyllum fabago populations involves the strengthening of the antioxidative pathways. Environ & Exp Botany, Vo. 150, June 2018, Pages 141-151.Aroca et al. (2015). S-sulfhydration: a new post-translational modification in plant systems. Plant Physiology March 2015 pp.00009.2015.Terrile et al. (2014). Nitric oxide-mediated cell death is triggered by chitosan in F. eumartii spores. Pest Manag Sci. 2014 Apr 25. doi: 10.1002/ps.3814.Tsaniklidis et al. (2013). L-Ascorbic acid metabolism in parthenocarpic and seeded cherry tomatoes. Plant Growth Regul,DOI 10.1007/s10725-013-9845-0. (Solanum lycopersicum, immunolocalization)
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA. Alternative name: Csn1.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at 4 C; Please remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
Cas9 from Streptococcus pyogenes
Immunogen:
Recombinant protein part from the N-terminus of Cas9 from Streptococcus pyogenes.
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA. Alternative name: Csn1.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. For long term storage -80°Cis recommended. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Cas9 from Streptococcus pyogenes
Immunogen:
Recombinant protein part from the N-terminus of Cas9 from Streptococcus pyogenes.
Applications:
Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Staphylococcus aureus
Expected Species:
Staphylococcus pyogenes UniProt: Q99ZW2
Immunogen:
KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6(peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.This antibody is directly conjugated to ALP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
Staphylococcus aureus
Expected Species:
Staphylococcus pyogenes UniProt: Q99ZW2
Immunogen:
KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6(peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.This antibody is directly conjugated to Biotin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
Staphylococcus aureus
Expected Species:
Staphylococcus pyogenes UniProt: Q99ZW2
Immunogen:
KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6(peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system, CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
Staphylococcus aureus
Expected Species:
Staphylococcus pyogenes UniProt: Q99ZW2
Immunogen:
KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6(peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system, CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
Staphylococcus aureus
Expected Species:
Staphylococcus pyogenes UniProt: Q99ZW2
Immunogen:
KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6(peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system, CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
Staphylococcus aureus
Expected Species:
Staphylococcus pyogenes UniProt: Q99ZW2
Immunogen:
KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6(peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.This antibody is directly conjugated to HRP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
Staphylococcus aureus
Expected Species:
Staphylococcus pyogenes UniProt: Q99ZW2
Immunogen:
KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6(peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
Bovine milk contains two types of beta-casein protein, A2 or A1. Recent studies have shown that milk containing the A1 beta casein protein can contribute to issues including gastrointestinal discomfort after ingestion. There is some evidence of a link between ingestion of A1 beta casein protein and the development of Type 1 diabetes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative. Refer to the product label for antibody concentration.
Host Animal:
Chicken
Species Reactivity:
Bovine
Immunogen:
A synthetic peptide (PGPIHNSLP, aa: 78-86) conjugated to KLH has been used as immunogen. Bovine A2 beta casein differs from bovine A1 beta casein by one amino acid (P82 -> H82)
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
Western blot and ELISA. Suggested working dilution for western blot is 1:1,000-1:5,000. The amount of milk per lane can be 0.05 µL-0.1 µL for Western blot. Sample Preparation: Milk should be diluted 1:10 in 0.1M NaOH. The reason for diluting (1:10) in 0.1M NaOH is because the milk protein is easier to dissolve in NaOH. The sample is then further diluted with PBS or other buffer and mixed at 1:1 ratio, to prepare for loading. For example, one can take the 1:10 dilution milk (0.5 µL-1 µL) and add into 9.5 µL or 9 µL PBS and mix with 10 µL SDS-PAGE Sample buffer, boil for 5 minutes, quick spin, and load on the gel. Recommended blocking buffer: TBS with 5% BSA. Recommended antibody dilution buffer: TBST containing 3% BSA. Biosensis recommends that optimal working dilutions should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody is specific to A1 beta casein by western blot. No cross-reactivity with A2 beta casein is seen. Species cross-reactivity not tested.
Storage:
Maintain unopened vial at -20°C for up to 12 months after date of receipt. After opening maintain at -20°C in undiluted aliquots for up to 6 months. For short-term storage, keep aliquot at 2-8°C for up to one week. Avoid repeated freeze-thaw cycles.
Bovine milk contains two types of beta-casein protein, A2 or A1. Recent studies have shown that milk containing the A1 beta casein protein can contribute to issues including gastrointestinal discomfort after ingestion. There is some evidence of a link between ingestion of A1 beta casein protein and the development of Type 1 diabetes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative. Refer to the product label for antibody concentration.
Host Animal:
Chicken
Species Reactivity:
Bovine
Immunogen:
A synthetic peptide (PGPIPNSLP, aa: 78-86) conjugated to KLH has been used as immunogen. Bovine A1 beta casein differs from bovine A2 beta casein by one amino acid (H82 -> P82)
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
Western blot and ELISA. Suggested working dilution for western blot is 1:200-1:1,000. The amount of milk per lane can be 0.05 µL-0.1 µL for Western blot. Sample Preparation: Milk should be diluted 1:10 in 0.1M NaOH. The reason for diluting (1:10) in 0.1M NaOH is because the milk protein is easier to dissolve in NaOH. The sample is then further diluted with PBS or other buffer and mixed at 1:1 ratio, to prepare for loading. For example, one can take the 1:10 dilution milk (0.5 µL-1 µL) and add into 9.5 µL or 9 µL PBS and mix with 10 µL SDS-PAGE Sample buffer, boil for 5 minutes, quick spin, and load on the gel. Recommended blocking buffer: TBS with 5% BSA. Recommended antibody dilution buffer: TBST containing 3% BSA. Biosensis recommends that optimal working dilutions should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The antibody is specific to A2 beta casein by western blot. No cross-reactivity with A1 beta casein is seen. Species cross-reactivity not tested.
Storage:
Maintain unopened vial at -20°C for up to 12 months after date of receipt. After opening maintain at -20°C in undiluted aliquots for up to 6 months. For short-term storage, keep aliquot at 2-8°C for up to one week. Avoid repeated freeze-thaw cycles.
PE-1831 is A1 beta casein protein that is in its native form and purified from bovine A1/A1 commercial cow's milk collected from cows that are certified BSE and disease free by the manufacture of the milk. IMPORTANT: This product may give different OD readings as compared to the protein calibrator contained in our A1 Casein ELISA Kit due to its formulation. Thus it is NOT recommended for use as a standard or QC marker in the A1 ELISA assays.
Background Info:
The protein named Casein is the largest group of proteins in mammalian milk, making up about > 80% of total protein content. Different mammals have different amounts of caseins in their milks and not all casein proteins are the same.<br><br>According to published literature, (PMID: 15453478), multiple forms of casein proteins exist in cow's milk, with beta-casein being one of the most predominant forms. Beta casein in domestic cows at lesat, has two alleles or isoforms. Beta A1 and Beta A2.<br><br>A1 beta-casein is found in milk from breeds of cows that originated in northern Europe. These breeds include Holstein, Friesian, Ayrshire, and British Shorthorn.<br><br>A2 beta-casein. is found in milk breeds that originated in the Channel Islands US and southern France. These include Guernsey, Jersey, Charolais, and Limousin cows (PMID: 15867940, PMID: 15453478).<br><br>Traditionally, store bought regular milk contains both A1 and A2 beta-casein proteins, but pure A2 milk can be obtained that originates only from A2 cows.<br><br>Some studies suggest that there could be some sort of a link between the A1 beta-casein and immunological dieseases such as type-1 diabetes and others and work is underway to understand how beta casein, both A1 and A2 may or may not contribute to human illness as well a health.
Product Type:
Protein
Format:
Liquid. Protein is provided as 50 µL aliquot at 0.4 mg/mL in TBS, pH 8.5, containing protease inhibitor. Does not contain preservatives. Use sterile methods when handling.
Applications:
WB
Application Details:
Western blotting (100 ng or less) as a standard, not recommended for ELISA. Other applications not yet tested.
Alternative Names:
Beta Casein; CSN2
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Protein is shipped on ice packs. Upon receipt, divide into aliquots and store product long-term at -20°C. Store working aliquots short-term at 2-8°C as undiluted liquid for one week. AVOID MULTIPLE FREEZE THAWS
Purification:
The product is >95% A1 beta casein based on SDS-PAGE gel. Affinity-purified, native protein
PE-1832 is A2 beta casein protein that is purified in its native form from bovine A2/A2 milk to >95% purity. The milk is collected from commercial cows certified free of disease and BSE by the manufacture of the milk. IMPORTANT: This product may give different OD readings as compared to the protein calibrator contained in our A2 Casein ELISA Kit due to its formulation. Thus it is NOT recommended for use as a standard or QC marker in the A2 ELISA assays.
Background Info:
The protein named Casein is the largest group of proteins in mammalian milk, making up about > 80% of total protein content. Different mammals have different amounts of caseins in their milks and not all casein proteins are the same.<br><br>According to published literature, (PMID: 15453478), multiple forms of casein proteins exist in cow's milk, with beta-casein being one of the most predominant forms. Beta casein in domestic cows at lesat, has two alleles or isoforms. Beta A1 and Beta A2.<br><br>A1 beta-casein is found in milk from breeds of cows that originated in northern Europe. These breeds include Holstein, Friesian, Ayrshire, and British Shorthorn.<br><br>A2 beta-casein. is found in milk breeds that originated in the Channel Islands US and southern France. These include Guernsey, Jersey, Charolais, and Limousin cows (PMID: 15867940, PMID: 15453478).<br><br>Traditionally, store bought regular milk contains both A1 and A2 beta-casein proteins, but pure A2 milk can be obtained that originates only from A2 cows.<br><br>Some studies suggest that there could be some sort of a link between the A1 beta-casein and immunological dieseases such as type-1 diabetes and others and work is underway to understand how beta casein, both A1 and A2 may or may not contribute to human illness as well a health.
Product Type:
Protein
Format:
Liquid. Protein is provided as 50 µL aliquot at 0.4 mg/mL in TBS, pH 8.5, containing protease inhibitor. Does not contain preservatives. Use sterile methods when handling.
Applications:
WB
Application Details:
Western blotting (100 ng or less) as a standard, not recommended for ELISA. Other applications not yet tested.
Alternative Names:
Beta Casein; CSN2
Biosensis Brand:
Biosensis®
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Storage:
Protein is shipped on ice packs. Upon receipt, divide into aliquots and store product long-term at -20°C. Store working aliquots short-term at 2-8°C as undiluted liquid for one week. AVOID MULTIPLE FREEZE THAWS
Chicken anti-Casein kinase I isoform alpha (CKI-alpha) Polyclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
Background Info:
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration. (Reference: uniprot.org)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal:
Chicken
Species Reactivity:
Bovine,Chicken,Horse,Human,Mouse,Pig,Rat
Immunogen:
Shortest isoform of recombinant full length CK1a.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB): 1:5,000 - 1:10,000. Casein kinase 1 alpha has a predicted molecular weight of 38 kDa. <br><br>Immunocytochemistry (ICC): 1:500-1:1,000.<br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CK1; CKI-alpha;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Antibody recognizes CK1 by western blot and immunohistochemistry. Cross reactivity
Storage:
After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Cat2 (Catalase 2) is an enzyme which occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Catalase is an enzyme found in most living organisms which is catalazying decomposition of hydrogen peroxide to water and oxygen.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii, Scenedesmus sp.
Expected Species:
Coccomyxa subellipsoidea C-169, Nannochloropsis gaditana, Ulva prolifera, Zosteria marina Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide chosen from Chlamydomonas reinhardtii catalase sequence, UniProt: A8J537
Catalase is an enzyme found in most living organisms which is catalazying decomposition of hydrogen peroxide to water and oxygen. In plant cells catalase is found in peroxisomes. This enzyme is involved in photorespiration and symbiotic nitrogen fixation.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Fragaria x ananassa,Arabidopsis thaliana, Aponogeton madagascariensis, Brassica napus, Brassica oleracea, Fragaria x ananassa, Hordeum vulgare, Lathyrus sativus, Lupinus albus, Lupinus luteus, Moniliophthora perniciosa, Musa acuminate, Musa paradisiaca L., Nicotiana bentamina, Nicotiana tabacum, Oryza sativa, Paulownia tomentosa, Picrorhiza kurroa, Pisum sativum, Plumbago zeylanica, Setaria italica L. P. Beauv, Solanum lycopersicum, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis vinifera
To obtain reactivity with Solanum lycopersicum urea gel needs to be apply. Please, contact us for more details.To decrease background signal this antibody needs to be incubated in PBS-T NOT TBS-T. For reference, check image in application example below.
Tokarz et al. (2021). Stem Photosynthesis-A Key Element of Grass Pea (Lathyrus sativus L.) Acclimatisation to Salinity. Int J Mol Sci. 2021 Jan 12;22(2):685. doi: 10.3390/ijms22020685. PMID: 33445673; PMCID: PMC7828162.Li et al. (2021) Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit. Hortic Res. 2021 Feb 1;8(1):31. doi: 10.1038/s41438-021-00470-w. PMID: 33518707; PMCID: PMC7848011.Wilmowicz et al (2021) EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers. International Journal of Molecular Sciences. 2021; 22(6):3001. https://doi.org/10.3390/ijms22063001Bapatla et al. (2021). Modulation of Photorespiratory Enzymes by Oxidative and Photo-Oxidative Stress Induced by Menadione in Leaves of Pea (Pisum sativum). Plants 10, no. 5: 987. https://doi.org/10.3390/plants10050987Adamiec et al. (2021). Fatty acid composition and cpDNA content in Arabidopsis thaliana mutants deprived of EGY1 protease. PHOTOSYNTHETICA 59 (4): 633-639, 2021, DOI 10.32615/ps.2021.053
Special application note:
This antibody is recognizing all three isoforms of Arabidopsis thaliana catalase. Catalase-2 is a main isoform expressed in leaf tissue and localized to peroxisomes. This antibody contains 0.1 % ProClin.
Sheep anti-Catenin beta Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Catenin beta is an adherens junction protein and has a role in the regulation of cell adhesion and in signal transduction through the Wnt pathway. At least 2 isoforms are produced by alternative splicing.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, and 0.05 % sodium azide.
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide (C-SNQLAWFDTDL) corresponding to the amino acid region 771-781 of human Catenin beta coupled to carrier protein KLH.
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB) and Immunoprecipitation. A concentration of 1.0 µg/mL is recommended for WB. Human Catenin beta (isoform 1) has a predicted length of 781 residues and MW of 86 kDa. A concentration of 5 µg/500 µg of lysate is recommended for immunoprecipitation. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB against the antigen. This antibody was also validated for immunoprecipitation in rat liver lysate (data not shown). Human; mouse; rat;
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Catenin beta is an adherens junction protein and has a role in the regulation of cell adhesion and in signal transduction through the Wnt pathway. At least 2 isoforms are produced by alternative splicing.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, and 0.05 % sodium azide.
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide (C-QSYLDSGIHSGATTTAPSL) corresponding to the amino acid region 28-46 of human Catenin beta coupled to carrier protein KLH. This antibody was designed to detect the active form of Catenin beta . The antibody exhibits a strong preference for dephosphorylated beta catenin protein by western blot but the antibody may show cross reactivity with phosphorylated catenin beta under certain conditions.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB) and Immunocytochemistry. A concentration of 2.0 µg/mL is recommended for WB. Human Catenin beta (isoform 1) has a predicted length of 781 residues and MW of 86 kDa. It has been observed as a 92 kDa band in SDS-reducing gels. A concentration of 4.0 µg/mL is recommended for Immunocytochemistry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rat;
Storage:
At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative:
0.05% (w/v) Sodium Azide
Storage:
2-8 °C
Country Of Origin:
Normal Cat Serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Disclaimer:
For in vitro Laboratory Use Only. Not for diagnostic or therapeutic use. Not for human or animal consumption. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc. Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc.
Normal cat serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added. Serum may may contain anti-feline calicivirus antibodies. Not suitable as negative control within calicivirus assay.
Normal cat serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 60,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Cat purified IgG contains Protein A purified cat IgG from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Cat purified IgG contains Protein A purified cat IgG from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
CBP20 (Nuclear cap-binding protein subunit 2)is a component of the cap-binding complex (CBC), involved in various processes such as pre-mRNA splicing and RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs). Alternative names: 20 kDa nuclear cap-binding protein, NCBP 20 kDa subunit
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Glycne max, Hordeum vulgare, Lotus corniculatus, Nicotiana tabacum, Oryza sativa, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Zea mays Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide, derived with Arabidopsis thaliana CBP20 protein Q9xFD1, At5g44200
CBP80 is a component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing. Alternative names: 80 kDa nuclear cap-binding protein, NCBP 80 kDa subunit, abscisic acid-hypersensitive protein 1, ABA-hypersensitive protein 1, Protein ENSALADA
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
CBP (CREB-binding protein homolog) is a transcriptional coactivator in multiple signal transduction pathways. It might play a role in variety of cellular processes, including differentiation, cellular proliferation, immune response, homeostasis, tumorigenesis, and erganogenesis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Drosophila melanogaster
Expected Species:
Drosophila melanogaster
Immunogen:
Recombinant CBP part, fused to GST, derived from Drosophila melanogaster protein sequence, UniProt: O01368
CBP tag comes from muscle myosin light-chain kinase and contains 26 amino acid residues with the molecular weight of 4 kDa. This tag is characterized by the relatively high affinity for calmodulin (CaM), which makes it possible to purify CBP-tagged proteins from crude cell extracts using of a resin with CaM affinity. This antibody allows detection of CBP-tagged proteins.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
CCA1 (Circadian clocl associated 1) encodes a transcriptional repressor that performs overlapping functions with LHY in a regulatory feedback loop that is closely associated with the circadian oscillator of Arabidopsis. Binds to the evening element in the promoter of TOC1 and represses TOC1 transcription. CCA1 and LHY colocalize in the nucleus and form heterodimers in vivo. CCA1 and LHY function synergistically in regulating circadian rhythms of Arabidopsis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CCA1 protein sequence, UniProt:P92973, TAIR:AT2G46830
Important note about protein extractionTranscription factors are best isolated by freezing nucelar extract in liquid nitrogen after addition of extraction buffer, followed by thawing directly at 100 C heating block, not at RT. Centrifugation for 5 minutes will remove cell debris. Protein concenration is measure ad rest is frozen in liquid Nitrogen, in aliquots. Frozen samples are thawed at RT and loaded as soon as they are in liquid form, without heating. Such precautions are necessary to take to be able to detect a transcription factor using antibodies.
Application Details:
1: 500 (Co-IP), 1 : 500 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
67 | 80 kDa
Not reactive in:
Oryza sativa
Selected references:
Hung er al. (2018). The Arabidopsis LDL1/2-HDA6 histone modification complex is functionally associated with CCA1/LHY in regulation of circadian clock genes. Nucleic Acids Res. 2018 Aug 14. doi: 10.1093/nar/gky749.
The CCK-8 Antibody was raised to sulphated CCK-8 (26-33) coupled to bovine thyroglobulin with glutaraldehyde. The antibody has a proven strong indirect immunofluorescent staining at a 1/100-1/200 dilution and a strong Biotin-Streptavidin/HRP immunostaining at a 1/500-1/1000 dilution in rat hypothalamus and spinal cord. The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 10-6M CCK-8 immunolabeling was completely abolished by pre-adsorption with CCK-8, gastrin 17 and gastrin 34. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: α-CGRP, -CGRP, neurotensin, somatostatin, substance P, leucine enkephalin, methionine enkephalin, VIP, neuropeptide Y, gastric inhibitory polypeptide, bombesin, glucagon, peptide YY, and FMRF amide.
Copper is an essential element for function of chloroplasts. It is a co-factor for superoxide dismutase (SOD) and for plastocyanin. Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets. In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site. The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Glycne max, Hordeum vulgare, Solanum tuberosum Species of your interest not listed? Contact us
Immunogen:
The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5, fused to His-tag. Overexpressed using pET28a vector in Bl21 E. coli (Cd+) and purified using His-column. After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Speth et al. (2013). RACK1 scaffold proteins influence miRNA abundance in Arabidopsis. Plant J. Aug 13.Abdel-Ghany et al (2005). .AtCCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7. FEBS Lett. 11:2307-12.
CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Immunogen:
U937 human Caucasian hystiocytic lymphoma cell line
Applications:
FC,IHC,ICC
Additional Info:
The antibody B-T47 recognizes CD107a, a 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane. _x000D_
Cluster of Differentiation 10 (CD10) is a cell surface metalloendopeptidase that cleaves and inactivates several peptide hormones including glucagon, enkephalins, and oxytocin. Also known as Common Acute Lymphoblastic Leukemia Antigen (CALLA), it is an important cell surface marker in the diagnosis of human ALL (Acute Lymphocytic Leukemia), and is found positive in precursor B lymphoblastic leukemia/lymphoma, angioimmunoblastic T-cell lymphoma, Burkitt's lymphoma, and follicular germinal center lymphoma. CD10 expression has also been reported in a variety of non-hematolymphoid tissues, particularly of the kidney. It is a useful aid in the diagnosis of various malignant tumours such as renal cell carcinoma, endometrial stromal sarcoma, and hepatocellular carcinoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC525
Antibody Isotype:
IgG1
GMDN Code:
56938
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Kidney, Lymph Node, Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 10 (CD10) is a cell surface metalloendopeptidase that cleaves and inactivates several peptide hormones including glucagon, enkephalins, and oxytocin. Also known as Common Acute Lymphoblastic Leukemia Antigen (CALLA), it is an important cell surface marker in the diagnosis of human ALL (Acute Lymphocytic Leukemia), and is found positive in precursor B lymphoblastic leukemia/lymphoma, angioimmunoblastic T-cell lymphoma, Burkitt's lymphoma, and follicular germinal center lymphoma. CD10 expression has also been reported in a variety of non-hematolymphoid tissues, particularly of the kidney. It is a useful aid in the diagnosis of various malignant tumours such as renal cell carcinoma, endometrial stromal sarcoma, and hepatocellular carcinoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC525
Antibody Isotype:
IgG1
GMDN Code:
56938
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Kidney, Lymph Node, Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 10 (CD10) is a cell surface metalloendopeptidase that cleaves and inactivates several peptide hormones including glucagon, enkephalins, and oxytocin. Also known as Common Acute Lymphoblastic Leukemia Antigen (CALLA), it is an important cell surface marker in the diagnosis of human ALL (Acute Lymphocytic Leukemia), and is found positive in precursor B lymphoblastic leukemia/lymphoma, angioimmunoblastic T-cell lymphoma, Burkitt's lymphoma, and follicular germinal center lymphoma. CD10 expression has also been reported in a variety of non-hematolymphoid tissues, particularly of the kidney. It is a useful aid in the diagnosis of various malignant tumours such as renal cell carcinoma, endometrial stromal sarcoma, and hepatocellular carcinoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC525
Antibody Isotype:
IgG1
GMDN Code:
56938
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Kidney, Lymph Node, Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
CD117 or Proto-oncogene c-Kit (c-Kit) is a member of the Tyrosine Kinase Receptor (TKR) family, and is an important cell surface marker found on hematopoietic stem cells, melanocytes, mast cells, Cajal cells, germ cells, basal cells of skin, and mammary ductal epithelia. It is considered an important marker in the diagnosis and classification of Gastrointestinal Stromal Tumours (GISTs), mast cell diseases, Acute Myeloid Leukemia (AML), Small Cell Lung Carcinoma (SCLC), and Ewing's sarcoma.
CD117 or Proto-oncogene c-Kit (c-Kit) is a member of the Tyrosine Kinase Receptor (TKR) family, and is an important cell surface marker found on hematopoietic stem cells, melanocytes, mast cells, Cajal cells, germ cells, basal cells of skin, and mammary ductal epithelia. It is considered an important marker in the diagnosis and classification of Gastrointestinal Stromal Tumours (GISTs), mast cell diseases, Acute Myeloid Leukemia (AML), Small Cell Lung Carcinoma (SCLC), and Ewing's sarcoma.
CD117 or Proto-oncogene c-Kit (c-Kit) is a member of the Tyrosine Kinase Receptor (TKR) family, and is an important cell surface marker found on hematopoietic stem cells, melanocytes, mast cells, Cajal cells, germ cells, basal cells of skin, and mammary ductal epithelia. It is considered an important marker in the diagnosis and classification of Gastrointestinal Stromal Tumours (GISTs), mast cell diseases, Acute Myeloid Leukemia (AML), Small Cell Lung Carcinoma (SCLC), and Ewing's sarcoma.
Cluster of Differentiation 13 (CD13) is a transmembrane protein that is overexpressed in both hematological and solid malignancies, including Acute Myeloid Leukemia (AML). Although hypogranular variants of AML are difficult to distinguish from other AML subtypes due to the morphology, the diagnosis of this variant is possible through using a panel of CD13, CD16, CD33, CD34, and CD117. Alternatively, a panel of CD13, CD34, CD43, CD68, CD117, CD163, lysozyme, and MPO is very useful for accurately diagnosing myeloid sarcoma and distinguishing it from large cell lymphoma, undifferentiated carcinoma, lymphoblastic lymphoma, malignant melanoma, Burkitt's lymphoma, extra-medullary hematopoiesis, and inflammation. Since CD13 is expressed in both normal and neoplastic liver tissues, CD13 staining is useful for distinguishing between hepatocellular carcinoma and non-hepatocellular neoplasms.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC123
Antibody Isotype:
IgG1
GMDN Code:
62795
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Liver
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 13 (CD13) is a transmembrane protein that is overexpressed in both hematological and solid malignancies, including Acute Myeloid Leukemia (AML). Although hypogranular variants of AML are difficult to distinguish from other AML subtypes due to the morphology, the diagnosis of this variant is possible through using a panel of CD13, CD16, CD33, CD34, and CD117. Alternatively, a panel of CD13, CD34, CD43, CD68, CD117, CD163, lysozyme, and MPO is very useful for accurately diagnosing myeloid sarcoma and distinguishing it from large cell lymphoma, undifferentiated carcinoma, lymphoblastic lymphoma, malignant melanoma, Burkitt's lymphoma, extra-medullary hematopoiesis, and inflammation. Since CD13 is expressed in both normal and neoplastic liver tissues, CD13 staining is useful for distinguishing between hepatocellular carcinoma and non-hepatocellular neoplasms.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC123
Antibody Isotype:
IgG1
GMDN Code:
62795
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Liver
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 13 (CD13) is a transmembrane protein that is overexpressed in both hematological and solid malignancies, including Acute Myeloid Leukemia (AML). Although hypogranular variants of AML are difficult to distinguish from other AML subtypes due to the morphology, the diagnosis of this variant is possible through using a panel of CD13, CD16, CD33, CD34, and CD117. Alternatively, a panel of CD13, CD34, CD43, CD68, CD117, CD163, lysozyme, and MPO is very useful for accurately diagnosing myeloid sarcoma and distinguishing it from large cell lymphoma, undifferentiated carcinoma, lymphoblastic lymphoma, malignant melanoma, Burkitt's lymphoma, extra-medullary hematopoiesis, and inflammation. Since CD13 is expressed in both normal and neoplastic liver tissues, CD13 staining is useful for distinguishing between hepatocellular carcinoma and non-hepatocellular neoplasms.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC123
Antibody Isotype:
IgG1
GMDN Code:
62795
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Liver
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
U266 human peripheral blood myeloma cell line
Applications:
FC,IHC
Additional Info:
The antibody B-A38 recognizes CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes. _x000D_
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
A mixture of U266 and XG-1 human myeloma cell lines
Applications:
FC,IP,WB,IHC
Additional Info:
The mouse monoclonal antibody MI15 recognizes CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes.
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
A mixture of U266 and XG-1 human myeloma cell lines
Applications:
FC,IP,WB,IHC
Additional Info:
The mouse monoclonal antibody MI15 recognizes CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes.
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
A mixture of U266 and XG-1 human myeloma cell lines
Applications:
FC,IP,WB,IHC
Additional Info:
The mouse monoclonal antibody MI15 recognizes CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes.
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
U266 human peripheral blood myeloma cell line
Applications:
FC,IHC
Additional Info:
The antibody B-A38 recognizes CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes. _x000D_
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Immunogen:
U266 human peripheral blood myeloma cell line
Applications:
FC,IHC
Additional Info:
The antibody B-A38 recognizes CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes. _x000D_
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
A mixture of U266 and XG-1 human myeloma cell lines
Applications:
FC,IP,WB,IHC
Additional Info:
The mouse monoclonal antibody MI15 recognizes CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes.
CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
A mixture of U266 and XG-1 human myeloma cell lines
Applications:
FC,IP,WB,IHC
Additional Info:
The mouse monoclonal antibody MI15 recognizes CD138 (syndecan 1), a 65-70 kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes.
Cluster of Differentiation 138 (CD138), also known as Syndecan-1, is a trans- membrane glycoprotein present on the surface of B-cells during late stage differentiation. Anti-CD138 is used to differentiate marginal zone lymphoma from lymphoplasmacytic lymphoma. ALK+ Large B-Cell Lymphoma (LBCL) commonly stains positively for CD138, but not for CD20 and CD79a. Anti-CD138 reacts positively with HHV8-associated primary effusion lymphoma that lacks B-cell markers. CD138 is also a useful marker for identifying and enumerating benign, reactive, or malignant plasma cells from the bone marrow biopsy samples.
Cluster of Differentiation 138 (CD138), also known as Syndecan-1, is a trans- membrane glycoprotein present on the surface of B-cells during late stage differentiation. Anti-CD138 is used to differentiate marginal zone lymphoma from lymphoplasmacytic lymphoma. ALK+ Large B-Cell Lymphoma (LBCL) commonly stains positively for CD138, but not for CD20 and CD79a. Anti-CD138 reacts positively with HHV8-associated primary effusion lymphoma that lacks B-cell markers. CD138 is also a useful marker for identifying and enumerating benign, reactive, or malignant plasma cells from the bone marrow biopsy samples.
Cluster of Differentiation 138 (CD138), also known as Syndecan-1, is a trans- membrane glycoprotein present on the surface of B-cells during late stage differentiation. Anti-CD138 is used to differentiate marginal zone lymphoma from lymphoplasmacytic lymphoma. ALK+ Large B-Cell Lymphoma (LBCL) commonly stains positively for CD138, but not for CD20 and CD79a. Anti-CD138 reacts positively with HHV8-associated primary effusion lymphoma that lacks B-cell markers. CD138 is also a useful marker for identifying and enumerating benign, reactive, or malignant plasma cells from the bone marrow biopsy samples.
Cluster of Differentiation 13 (CD13) is a transmembrane protein that is overexpressed in both hematological and solid malignancies, including Acute Myeloid Leukemia (AML). Although hypogranular variants of AML are difficult to distinguish from other AML subtypes due to the morphology, the diagnosis of this variant is possible through using a panel of CD13, CD16, CD33, CD34, and CD117. Alternatively, a panel of CD13, CD34, CD43, CD68, CD117, CD163, lysozyme, and MPO is very useful for accurately diagnosing myeloid sarcoma and distinguishing it from large cell lymphoma, undifferentiated carcinoma, lymphoblastic lymphoma, malignant melanoma, Burkitt's lymphoma, extra-medullary hematopoiesis, and inflammation. Since CD13 is expressed in both normal and neoplastic liver tissues, CD13 staining is useful for distinguishing between hepatocellular carcinoma and non-hepatocellular neoplasms.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC119
Antibody Isotype:
IgG1
GMDN Code:
56944
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Liver
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 13 (CD13) is a transmembrane protein that is overexpressed in both hematological and solid malignancies, including Acute Myeloid Leukemia (AML). Although hypogranular variants of AML are difficult to distinguish from other AML subtypes due to the morphology, the diagnosis of this variant is possible through using a panel of CD13, CD16, CD33, CD34, and CD117. Alternatively, a panel of CD13, CD34, CD43, CD68, CD117, CD163, lysozyme, and MPO is very useful for accurately diagnosing myeloid sarcoma and distinguishing it from large cell lymphoma, undifferentiated carcinoma, lymphoblastic lymphoma, malignant melanoma, Burkitt's lymphoma, extra-medullary hematopoiesis, and inflammation. Since CD13 is expressed in both normal and neoplastic liver tissues, CD13 staining is useful for distinguishing between hepatocellular carcinoma and non-hepatocellular neoplasms.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC119
Antibody Isotype:
IgG1
GMDN Code:
56944
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Liver
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 13 (CD13) is a transmembrane protein that is overexpressed in both hematological and solid malignancies, including Acute Myeloid Leukemia (AML). Although hypogranular variants of AML are difficult to distinguish from other AML subtypes due to the morphology, the diagnosis of this variant is possible through using a panel of CD13, CD16, CD33, CD34, and CD117. Alternatively, a panel of CD13, CD34, CD43, CD68, CD117, CD163, lysozyme, and MPO is very useful for accurately diagnosing myeloid sarcoma and distinguishing it from large cell lymphoma, undifferentiated carcinoma, lymphoblastic lymphoma, malignant melanoma, Burkitt's lymphoma, extra-medullary hematopoiesis, and inflammation. Since CD13 is expressed in both normal and neoplastic liver tissues, CD13 staining is useful for distinguishing between hepatocellular carcinoma and non-hepatocellular neoplasms.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC119
Antibody Isotype:
IgG1
GMDN Code:
56944
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Liver
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The CD14 [IHC014] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
The CD14 [IHC014] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
The CD14 [IHC014] antibody is intended for qualified laboratories to qualitatively identify by light microscopy, the presence of associated antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections using immunohistochemistry test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of neoplastic tissues within the context of antibody panels, the patients clinical history and other diagnostic tests as evaluated by a qualified pathologist.
Cluster of Differentiation 15 (CD15), also known as Leu-M1, is a carbohydrate adhesion molecule. Positive staining for CD15 and negative staining for leukocyte common antigen or other B- or T-cell lineage markers helps recognize Reed Sternberg cells (RSC) in classic Hodgkin's lymphoma, and distinguishes it from Hodgkin-like neoplasms. CD15 does not stain mesotheliomas and is therefore most useful for distinguishing epithelial mesothelioma from adenocarcinoma.
Cluster of Differentiation 15 (CD15), also known as Leu-M1, is a carbohydrate adhesion molecule. Positive staining for CD15 and negative staining for leukocyte common antigen or other B- or T-cell lineage markers helps recognize Reed Sternberg cells (RSC) in classic Hodgkin's lymphoma, and distinguishes it from Hodgkin-like neoplasms. CD15 does not stain mesotheliomas and is therefore most useful for distinguishing epithelial mesothelioma from adenocarcinoma.
Cluster of Differentiation 15 (CD15), also known as Leu-M1, is a carbohydrate adhesion molecule. Positive staining for CD15 and negative staining for leukocyte common antigen or other B- or T-cell lineage markers helps recognize Reed Sternberg cells (RSC) in classic Hodgkin's lymphoma, and distinguishes it from Hodgkin-like neoplasms. CD15 does not stain mesotheliomas and is therefore most useful for distinguishing epithelial mesothelioma from adenocarcinoma.
Cluster of Differentiation 163 (CD163) is a receptor found exclusively on the surface of monocytes and macrophages. The solubilized form in plasma is upregulated in inflammatory diseases such as rheumatoid arthritis, atherosclerosis, and Gaucher’s disease, which supports recent studies that have found IL-10, glucocorticoids, and other inflammatory modulators to upregulate CD163 expression. CD163 staining is useful for differentiating synovial intimal fibroblasts from synovial macrophages in rheumatoid arthritis. Overexpression of CD163 is also present in patients with myelomonocytic leukemia dealing with microbial infections. CD163 expression is found in leukemias with monocytic differentiation and synovial-type giant cell tumours of the vertebral column.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC163
Antibody Isotype:
IgG1
GMDN Code:
62796
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Inflamed Tissue
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 163 (CD163) is a receptor found exclusively on the surface of monocytes and macrophages. The solubilized form in plasma is upregulated in inflammatory diseases such as rheumatoid arthritis, atherosclerosis, and Gaucher’s disease, which supports recent studies that have found IL-10, glucocorticoids, and other inflammatory modulators to upregulate CD163 expression. CD163 staining is useful for differentiating synovial intimal fibroblasts from synovial macrophages in rheumatoid arthritis. Overexpression of CD163 is also present in patients with myelomonocytic leukemia dealing with microbial infections. CD163 expression is found in leukemias with monocytic differentiation and synovial-type giant cell tumours of the vertebral column.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC163
Antibody Isotype:
IgG1
GMDN Code:
62796
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Inflamed Tissue
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 163 (CD163) is a receptor found exclusively on the surface of monocytes and macrophages. The solubilized form in plasma is upregulated in inflammatory diseases such as rheumatoid arthritis, atherosclerosis, and Gaucher’s disease, which supports recent studies that have found IL-10, glucocorticoids, and other inflammatory modulators to upregulate CD163 expression. CD163 staining is useful for differentiating synovial intimal fibroblasts from synovial macrophages in rheumatoid arthritis. Overexpression of CD163 is also present in patients with myelomonocytic leukemia dealing with microbial infections. CD163 expression is found in leukemias with monocytic differentiation and synovial-type giant cell tumours of the vertebral column.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC163
Antibody Isotype:
IgG1
GMDN Code:
62796
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Inflamed Tissue
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 16 (CD16) is a receptor on natural killer cells, neutrophils, monocytes, and macrophages. CD16 binds the Fc portion of antibodies to activate these immune cells. CD16 staining is useful in the differential diagnosis of hepatosplenic gamma delta T-cell lymphoma and gamma delta T-cell large granular lymphocyte leukemia from mucosal and cutaneous gamma delta T-cell lymphoma. Likely due to dysgranulopoiesis, granulocytes with myelomonocytic leukemia have decreased CD16 expression in comparison to granulocytes with chronic myelogenous leukemia and control bone marrow biopsies.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC528
Antibody Isotype:
IgG1
GMDN Code:
56950
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Liver
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 16 (CD16) is a receptor on natural killer cells, neutrophils, monocytes, and macrophages. CD16 binds the Fc portion of antibodies to activate these immune cells. CD16 staining is useful in the differential diagnosis of hepatosplenic gamma delta T-cell lymphoma and gamma delta T-cell large granular lymphocyte leukemia from mucosal and cutaneous gamma delta T-cell lymphoma. Likely due to dysgranulopoiesis, granulocytes with myelomonocytic leukemia have decreased CD16 expression in comparison to granulocytes with chronic myelogenous leukemia and control bone marrow biopsies.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC528
Antibody Isotype:
IgG1
GMDN Code:
56950
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Liver
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 16 (CD16) is a receptor on natural killer cells, neutrophils, monocytes, and macrophages. CD16 binds the Fc portion of antibodies to activate these immune cells. CD16 staining is useful in the differential diagnosis of hepatosplenic gamma delta T-cell lymphoma and gamma delta T-cell large granular lymphocyte leukemia from mucosal and cutaneous gamma delta T-cell lymphoma. Likely due to dysgranulopoiesis, granulocytes with myelomonocytic leukemia have decreased CD16 expression in comparison to granulocytes with chronic myelogenous leukemia and control bone marrow biopsies.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC528
Antibody Isotype:
IgG1
GMDN Code:
56950
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Liver
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
CD173 (blood group antigen H2) is a fucosylated saccharide (Fuc-alpha-1-2-Gal-beta-1-4-GlcNAc-beta) generated by beta-D-galactoside 2-alpha-L-fucosyltransferase (FUT1). CD173 belongs to markers of early hematopoiesis; it is expressed mainly on CD34-positive hematopoietic progenitor cells. CD173 is a precursor structure of CD174 (Lewis Y) and is also structurally related to CD15 (Lewis X). On endothelial cells CD173 and CD174 are concentrated on pseudopodial extensions responsible for initial contacts between endothelial cells.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Immunogen:
Human thrombocytes
Applications:
FC
Additional Info:
The antibody MEM-198 reacts with CD173 (H2), an extracellular saccharide antigen expressed mainly during early hematopoiesis; it is also expressed on endothelial cells.
Cluster of Differentiation 19 (CD19) is a surface receptor found on follicular dendritic cells and B-cells. CD19 is found on normal and malignant B-cells, and is known as a reliable marker for B-cells throughout its maturation stages. Anti-CD19 reacts positively with the mantle zone cells, scattered cells, and germinal centers of normal lymph tissues. Although CD20 and CD22 have similar staining patterns to CD19, CD19 is useful because it is also expressed in immature B-cells.
Cluster of Differentiation 19 (CD19) is a surface receptor found on follicular dendritic cells and B-cells. CD19 is found on normal and malignant B-cells, and is known as a reliable marker for B-cells throughout its maturation stages. Anti-CD19 reacts positively with the mantle zone cells, scattered cells, and germinal centers of normal lymph tissues. Although CD20 and CD22 have similar staining patterns to CD19, CD19 is useful because it is also expressed in immature B-cells.
Cluster of Differentiation 19 (CD19) is a surface receptor found on follicular dendritic cells and B-cells. CD19 is found on normal and malignant B-cells, and is known as a reliable marker for B-cells throughout its maturation stages. Anti-CD19 reacts positively with the mantle zone cells, scattered cells, and germinal centers of normal lymph tissues. Although CD20 and CD22 have similar staining patterns to CD19, CD19 is useful because it is also expressed in immature B-cells.
CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Human thymocytes
Applications:
FC,IP
Additional Info:
The mouse monoclonal antibody SK9 recognizes CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas.
CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Human thymocytes
Applications:
FC,IP
Additional Info:
The mouse monoclonal antibody SK9 recognizes CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas.
CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Human thymocytes
Applications:
FC,IP
Additional Info:
The mouse monoclonal antibody SK9 recognizes CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas.
CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Human thymocytes
Applications:
FC,IP
Additional Info:
The mouse monoclonal antibody SK9 recognizes CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas.
CD1a is part of a heterodimer with β-2-microglobulin, and mediates the capture and presentation of antigens, primarily lipid and glycolipid antigens of self or microbial origin, to T-cells. CD1a is expressed on interdigitating and dermal dendritic cells, veiled cells, Langerhans cells, antigen-presenting cells of the lymph nodes, and cortical thymocytes. Anti-CD1a stains Langerhans cell histiocytosis and cortical T LBL/L pre-T lymphoblastic lymphoma and leukemia. In concert with S100 and CD68, CD1a is very useful for differentiating Rosai-Dorfman disease from other histiocytic diseases.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC530
Antibody Isotype:
IgG1
GMDN Code:
56922
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Skin, Thymus
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
CD1a is part of a heterodimer with β-2-microglobulin, and mediates the capture and presentation of antigens, primarily lipid and glycolipid antigens of self or microbial origin, to T-cells. CD1a is expressed on interdigitating and dermal dendritic cells, veiled cells, Langerhans cells, antigen-presenting cells of the lymph nodes, and cortical thymocytes. Anti-CD1a stains Langerhans cell histiocytosis and cortical T LBL/L pre-T lymphoblastic lymphoma and leukemia. In concert with S100 and CD68, CD1a is very useful for differentiating Rosai-Dorfman disease from other histiocytic diseases.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC530
Antibody Isotype:
IgG1
GMDN Code:
56922
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Skin, Thymus
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
CD1a is part of a heterodimer with β-2-microglobulin, and mediates the capture and presentation of antigens, primarily lipid and glycolipid antigens of self or microbial origin, to T-cells. CD1a is expressed on interdigitating and dermal dendritic cells, veiled cells, Langerhans cells, antigen-presenting cells of the lymph nodes, and cortical thymocytes. Anti-CD1a stains Langerhans cell histiocytosis and cortical T LBL/L pre-T lymphoblastic lymphoma and leukemia. In concert with S100 and CD68, CD1a is very useful for differentiating Rosai-Dorfman disease from other histiocytic diseases.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC530
Antibody Isotype:
IgG1
GMDN Code:
56922
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Skin, Thymus
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
RAMOS human lymphoma cell line
Applications:
FC,IHC
Additional Info:
The antibody MEM-269 reacts with CD20 (Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.
Cluster of Differentiation (CD20), also known as B-Lymphocyte Antigen, is a non-glycosylated protein expressed on the surface of normal and malignant B-cells, which functions in chemokine signaling and microenvironmental interactions of B-cells. Anti-CD20 stains a minority of Reed-Sternberg cells with Hodgkin's disease. Since CD20 does not stain T-cell malignancies, it is a very useful marker for B-cell lymphomas. CD20 is also not reactive on non-hematopoietic neoplasms.
Cluster of Differentiation (CD20), also known as B-Lymphocyte Antigen, is a non-glycosylated protein expressed on the surface of normal and malignant B-cells, which functions in chemokine signaling and microenvironmental interactions of B-cells. Anti-CD20 stains a minority of Reed-Sternberg cells with Hodgkin's disease. Since CD20 does not stain T-cell malignancies, it is a very useful marker for B-cell lymphomas. CD20 is also not reactive on non-hematopoietic neoplasms.
Cluster of Differentiation (CD20), also known as B-Lymphocyte Antigen, is a non-glycosylated protein expressed on the surface of normal and malignant B-cells, which functions in chemokine signaling and microenvironmental interactions of B-cells. Anti-CD20 stains a minority of Reed-Sternberg cells with Hodgkin's disease. Since CD20 does not stain T-cell malignancies, it is a very useful marker for B-cell lymphomas. CD20 is also not reactive on non-hematopoietic neoplasms.
Cluster of Differentiation 21 (CD21) is a glycoprotein on the surface of B-cells that is bound by Epstein-Barr virus (EBV) during infection of these cells. CD21 staining is useful for recognizing follicular dendritic cell matrices in normal tonsillar and lymph tissue, and can also stain dendritic cell sarcomas. CD21 is also useful for distinguishing marginal zone lymphoma with follicular involvement from follicular lymphoma with marginal zone differentiation. When used in concert with other B- and T-cell markers, CD21 is valuable for differentiating between nodular lymphocyte-predominant Hodgkin's lymphoma, T-cell/histiocyte-rich B-cell lymphoma, and lymphocyte-rich classic Hodgkin's lymphoma. CD21 staining is useful for recognizing abnormal follicular dendritic cell patterns in angioimmunoblastic T-cell lymphoma and follicular T-cell lymphoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC533
Antibody Isotype:
IgG2a
GMDN Code:
56956
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Tonsil, Lymph Node
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 21 (CD21) is a glycoprotein on the surface of B-cells that is bound by Epstein-Barr virus (EBV) during infection of these cells. CD21 staining is useful for recognizing follicular dendritic cell matrices in normal tonsillar and lymph tissue, and can also stain dendritic cell sarcomas. CD21 is also useful for distinguishing marginal zone lymphoma with follicular involvement from follicular lymphoma with marginal zone differentiation. When used in concert with other B- and T-cell markers, CD21 is valuable for differentiating between nodular lymphocyte-predominant Hodgkin's lymphoma, T-cell/histiocyte-rich B-cell lymphoma, and lymphocyte-rich classic Hodgkin's lymphoma. CD21 staining is useful for recognizing abnormal follicular dendritic cell patterns in angioimmunoblastic T-cell lymphoma and follicular T-cell lymphoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC533
Antibody Isotype:
IgG2a
GMDN Code:
56956
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Tonsil, Lymph Node
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 21 (CD21) is a glycoprotein on the surface of B-cells that is bound by Epstein-Barr virus (EBV) during infection of these cells. CD21 staining is useful for recognizing follicular dendritic cell matrices in normal tonsillar and lymph tissue, and can also stain dendritic cell sarcomas. CD21 is also useful for distinguishing marginal zone lymphoma with follicular involvement from follicular lymphoma with marginal zone differentiation. When used in concert with other B- and T-cell markers, CD21 is valuable for differentiating between nodular lymphocyte-predominant Hodgkin's lymphoma, T-cell/histiocyte-rich B-cell lymphoma, and lymphocyte-rich classic Hodgkin's lymphoma. CD21 staining is useful for recognizing abnormal follicular dendritic cell patterns in angioimmunoblastic T-cell lymphoma and follicular T-cell lymphoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC533
Antibody Isotype:
IgG2a
GMDN Code:
56956
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Tonsil, Lymph Node
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Whole hairy cell leukemia cells and membrane preparation
Applications:
FC
Additional Info:
The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes._x000D_ <br><b>HLDA IV; WS Code B48</b>
CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Whole hairy cell leukemia cells and membrane preparation
Applications:
FC
Additional Info:
The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes._x000D_ <br><b>HLDA IV; WS Code B48</b>
CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Whole hairy cell leukemia cells and membrane preparation
Applications:
FC
Additional Info:
The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes._x000D_ <br><b>HLDA IV; WS Code B48</b>
CD23 (Fc epsilon RII), the low affinity IgE receptor, is a 45 kDa type II membrane glycoprotein expressed more or less on eosinophils, follicular dendritic cells, Langerhans cells, mature B cells (mainly upon activation), EBV-transformed lymphoblasts, monocytes, and subpopulation of platelets. A soluble form of 37 kDa and other its fragments were also described. CD23 mediates IgE-dependent cytotoxicity by eosinophils and macrophages, and downregulates IgE secretion in response to high levels of IgE, involving release of pro-inflammatory cytokines.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
EBV-transformed human cells
Applications:
FC,IP
Additional Info:
The mouse monoclonal antibody EBVCS-5 recognizes an epitope located in the stalk region of human low affinity IgE receptor (CD23) between the 37 and 25 kDa cleavage sites.
CD23 (Fc epsilon RII), the low affinity IgE receptor, is a 45 kDa type II membrane glycoprotein expressed more or less on eosinophils, follicular dendritic cells, Langerhans cells, mature B cells (mainly upon activation), EBV-transformed lymphoblasts, monocytes, and subpopulation of platelets. A soluble form of 37 kDa and other its fragments were also described. CD23 mediates IgE-dependent cytotoxicity by eosinophils and macrophages, and downregulates IgE secretion in response to high levels of IgE, involving release of pro-inflammatory cytokines.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
EBV-transformed human cells
Applications:
FC,IP
Additional Info:
The mouse monoclonal antibody EBVCS-5 recognizes an epitope located in the stalk region of human low affinity IgE receptor (CD23) between the 37 and 25 kDa cleavage sites.
CD23 (Fc epsilon RII), the low affinity IgE receptor, is a 45 kDa type II membrane glycoprotein expressed more or less on eosinophils, follicular dendritic cells, Langerhans cells, mature B cells (mainly upon activation), EBV-transformed lymphoblasts, monocytes, and subpopulation of platelets. A soluble form of 37 kDa and other its fragments were also described. CD23 mediates IgE-dependent cytotoxicity by eosinophils and macrophages, and downregulates IgE secretion in response to high levels of IgE, involving release of pro-inflammatory cytokines.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
EBV-transformed human cells
Applications:
FC,IP
Additional Info:
The mouse monoclonal antibody EBVCS-5 recognizes an epitope located in the stalk region of human low affinity IgE receptor (CD23) between the 37 and 25 kDa cleavage sites.
CD23 (Fc epsilon RII), the low affinity IgE receptor, is a 45 kDa type II membrane glycoprotein expressed more or less on eosinophils, follicular dendritic cells, Langerhans cells, mature B cells (mainly upon activation), EBV-transformed lymphoblasts, monocytes, and subpopulation of platelets. A soluble form of 37 kDa and other its fragments were also described. CD23 mediates IgE-dependent cytotoxicity by eosinophils and macrophages, and downregulates IgE secretion in response to high levels of IgE, involving release of pro-inflammatory cytokines.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
EBV-transformed human cells
Applications:
FC,IP
Additional Info:
The mouse monoclonal antibody EBVCS-5 recognizes an epitope located in the stalk region of human low affinity IgE receptor (CD23) between the 37 and 25 kDa cleavage sites.
Cluster of Differentiation 23 (CD23) is found on interleukin-4 activated B-cells, activated macrophages, eosinophils, and follicular dendritic cells, and is a receptor for IgE, an antibody involved in parasitic immunity. CD23 is present on Reed-Sternberg cells in Hodgkin's disease. Follicular dendritic cells and activated B-lymphocytes produce strong staining in germinal centers and weak patterns in mantle zone B-cells. CD23 is helpful in differentiating chronic lymphocytic leukemia from mantle cell leukemia. Small B-cell lymphomas are sometimes positive, while precursor B- and T-lymphomas, myeloid neoplasms, and mature T-cell lymphomas stain negatively with Anti-CD23.
Cluster of Differentiation 23 (CD23) is found on interleukin-4 activated B-cells, activated macrophages, eosinophils, and follicular dendritic cells, and is a receptor for IgE, an antibody involved in parasitic immunity. CD23 is present on Reed-Sternberg cells in Hodgkin's disease. Follicular dendritic cells and activated B-lymphocytes produce strong staining in germinal centers and weak patterns in mantle zone B-cells. CD23 is helpful in differentiating chronic lymphocytic leukemia from mantle cell leukemia. Small B-cell lymphomas are sometimes positive, while precursor B- and T-lymphomas, myeloid neoplasms, and mature T-cell lymphomas stain negatively with Anti-CD23.
Cluster of Differentiation 23 (CD23) is found on interleukin-4 activated B-cells, activated macrophages, eosinophils, and follicular dendritic cells, and is a receptor for IgE, an antibody involved in parasitic immunity. CD23 is present on Reed-Sternberg cells in Hodgkin's disease. Follicular dendritic cells and activated B-lymphocytes produce strong staining in germinal centers and weak patterns in mantle zone B-cells. CD23 is helpful in differentiating chronic lymphocytic leukemia from mantle cell leukemia. Small B-cell lymphomas are sometimes positive, while precursor B- and T-lymphomas, myeloid neoplasms, and mature T-cell lymphomas stain negatively with Anti-CD23.
CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
B6.1 CTL cell line
Applications:
FC,IP,IHC,FA
Additional Info:
The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4<sup>+</sup> T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
B6.1 CTL cell line
Applications:
FC,IP,IHC,FA
Additional Info:
The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4<sup>+</sup> T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
B6.1 CTL cell line
Applications:
FC,IP,IHC,FA
Additional Info:
The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4<sup>+</sup> T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
B6.1 CTL cell line
Applications:
FC,IP,IHC,FA
Additional Info:
The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4<sup>+</sup> T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
B6.1 CTL cell line
Applications:
FC,IP,IHC,FA
Additional Info:
The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4<sup>+</sup> T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
TRAIL-R2 (CD262, DR5) is one of two TNF superfamily member intracellular death domain containing receptors for TRAIL (APO2L). Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including tumor necrosis factor (TNF) and Fas ligand in the TNF family through their death domain containing receptors, TNF receptor 1 (TNFR1) and Fas, respectively. Another member in the TNF family has been identified and designated TRAIL (for TNF related apoptosis inducing ligand) and Apo2L (for Apo2 ligand). Receptors for TRAIL include two death domain containing receptors, DR4 and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. DcR1 (also called TRID), like the related death receptors DR4 and DR5, contains two extracellular cysteine rich domains. However, DcR1 contains no intracellular death domain and is thus incapable of signaling apoptosis. It has been suggested DcR1 is responsible for TRAIL resistance in normal human tissues including heart, placenta, lung, liver, kidney, spleen, and bone marrow. DR5 is a member of the TNF receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor related apoptosis inducing ligand (TNFSF10/TRAIL/APO2L), and transduces apoptosis signal. Studies with FADD deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Recombinant fusion protein of human IgG heavy chain and extracellular domain of DR5.
Applications:
FC
Additional Info:
The antibody DR5-01-1 recognizes an extracellular domain of TRAIL-R2 (DR5). TRAIL-R2 is one of two TNF superfamily member intracellular death domain containing receptors for TRAIL (APO2L). _x000D_ _x000D_
TRAIL-R3 (CD263, TR3, DcR1, LIT, TRID), expressed mainly on neutrophils, belongs to receptors of TRAIL, a TNF-like membrane cytotoxic protein that induces apoptosis in many tumour cells, but not in normal cells. TRAIL-R3, however, is a GPI-anchored protein that lacks cytoplasmic death domain, thus it is unable to induce apoptosis and serves as a negative regulator of apoptotic signaling by competing for binding of TRAIL with death receptor 5 (DR5).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
TRAIL-R3 (aa 1-280) - hIgGhc fusion protein
Applications:
FC
Additional Info:
The antibody TRAIL-R3-02 reacts with TRAIL-R3, a 35 kDa GPI-anchored extracellular membrane protein expressed mainly on neutrophils.
CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells._x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Mouse T cell lymphoma EL-4 cells_x000D_
Applications:
FC,IP,IHC,ICC,FA
Additional Info:
The Syrian hamster monoclonal antibody 37.51 reacts with extracellular domain of mouse CD28 costimulatory receptor, a 45 kDa homodimeric protein expressed by thymocytes, mature T cells and NK cells._x000D_
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation._x000D_ The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Human thymocytes
Applications:
FC,WB,IHC,ICC
Additional Info:
The mouse monoclonal antibody SK7 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody reacts with the epsilon chain of the CD3 complex. The monoclonal antibodies SK7 and UCHT1 recognize overlapping epitopes._x000D_ <br><b>HLDA II; WS Code T118<br>_x000D_ HLDA III; WS Code T492</b>
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation._x000D_ The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Human thymocytes
Applications:
FC,WB,IHC,ICC
Additional Info:
The mouse monoclonal antibody SK7 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody reacts with the epsilon chain of the CD3 complex. The monoclonal antibodies SK7 and UCHT1 recognize overlapping epitopes._x000D_ <br><b>HLDA II; WS Code T118<br>_x000D_ HLDA III; WS Code T492</b>
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation._x000D_ The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Human thymocytes
Applications:
FC,WB,IHC,ICC
Additional Info:
The mouse monoclonal antibody SK7 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody reacts with the epsilon chain of the CD3 complex. The monoclonal antibodies SK7 and UCHT1 recognize overlapping epitopes._x000D_ <br><b>HLDA II; WS Code T118<br>_x000D_ HLDA III; WS Code T492</b>
Cluster of Differentiation 30 (CD30) is a transmembrane cytokine receptor expressed by activated T- and B- cells. It is present on Reed-Sternberg cells in Hodgkin's lymphoma, most anaplastic large cell lymphomas, embryonal carcinomas, and primary cutaneous CD30 positive T-cell lymphoproliferative disorders. B-cell lymphomas are sometimes stained by Anti-CD30. Lymphomas exhibit Golgi zone accentuation when stained with Anti-CD30, while embryonal carcinomas produce membranous stains.
Cluster of Differentiation 30 (CD30) is a transmembrane cytokine receptor expressed by activated T- and B- cells. It is present on Reed-Sternberg cells in Hodgkin's lymphoma, most anaplastic large cell lymphomas, embryonal carcinomas, and primary cutaneous CD30 positive T-cell lymphoproliferative disorders. B-cell lymphomas are sometimes stained by Anti-CD30. Lymphomas exhibit Golgi zone accentuation when stained with Anti-CD30, while embryonal carcinomas produce membranous stains.
Cluster of Differentiation 30 (CD30) is a transmembrane cytokine receptor expressed by activated T- and B- cells. It is present on Reed-Sternberg cells in Hodgkin's lymphoma, most anaplastic large cell lymphomas, embryonal carcinomas, and primary cutaneous CD30 positive T-cell lymphoproliferative disorders. B-cell lymphomas are sometimes stained by Anti-CD30. Lymphomas exhibit Golgi zone accentuation when stained with Anti-CD30, while embryonal carcinomas produce membranous stains.
CD317, also known as BST2, tetherin, HM1.2 antigen, DAMP-2, is an integral transmembrane glycoprotein which may play a role in pre-B-cell growth, rheumatoid arthritis, and in antiretroviral defense, that blocks release of retrovirus from the cell surface. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells.
CD317, also known as BST2, tetherin, HM1.2 antigen, DAMP-2, is an integral transmembrane glycoprotein which may play a role in pre-B-cell growth, rheumatoid arthritis, and in antiretroviral defense, that blocks release of retrovirus from the cell surface. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells.
CD317, also known as BST2, tetherin, HM1.2 antigen, DAMP-2, is an integral transmembrane glycoprotein which may play a role in pre-B-cell growth, rheumatoid arthritis, and in antiretroviral defense, that blocks release of retrovirus from the cell surface. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells.
Cluster of Differentiation 31 (CD31) is present on hematopoietic stem cells (HSCs), and its expression is used to determine the concentration of HSCs in research studies and for bone marrow transplantation. Anti-CD31 is very specific and sensitive for endothelial cells and does not stain non-vascular tumours, therefore CD31 staining is used to recognize the vascular origins of neoplasms.
Cluster of Differentiation 31 (CD31) is present on hematopoietic stem cells (HSCs), and its expression is used to determine the concentration of HSCs in research studies and for bone marrow transplantation. Anti-CD31 is very specific and sensitive for endothelial cells and does not stain non-vascular tumours, therefore CD31 staining is used to recognize the vascular origins of neoplasms.
Cluster of Differentiation 31 (CD31) is present on hematopoietic stem cells (HSCs), and its expression is used to determine the concentration of HSCs in research studies and for bone marrow transplantation. Anti-CD31 is very specific and sensitive for endothelial cells and does not stain non-vascular tumours, therefore CD31 staining is used to recognize the vascular origins of neoplasms.
Cluster of Differentiation 34 (CD34) is a transmembrane glycoprotein expressed on hematopoietic stem and progenitor cells, vascular endothelium, embryonic fibroblasts, and rare glial cells in nervous tissue. CD34 is the most used marker for characterizing blasts in leukemia. CD34 is also present in some soft tissue tumours including solitary fibrous tumours and gastrointestinal stromal tumours. Proliferating endothelial cells seem to upregulate CD34 expression. Although specificity is low, Anti-CD34 reacts positively with more than 85% of angiosarcoma and Kaposi’s sarcoma.
Cluster of Differentiation 34 (CD34) is a transmembrane glycoprotein expressed on hematopoietic stem and progenitor cells, vascular endothelium, embryonic fibroblasts, and rare glial cells in nervous tissue. CD34 is the most used marker for characterizing blasts in leukemia. CD34 is also present in some soft tissue tumours including solitary fibrous tumours and gastrointestinal stromal tumours. Proliferating endothelial cells seem to upregulate CD34 expression. Although specificity is low, Anti-CD34 reacts positively with more than 85% of angiosarcoma and Kaposi’s sarcoma.
Cluster of Differentiation 34 (CD34) is a transmembrane glycoprotein expressed on hematopoietic stem and progenitor cells, vascular endothelium, embryonic fibroblasts, and rare glial cells in nervous tissue. CD34 is the most used marker for characterizing blasts in leukemia. CD34 is also present in some soft tissue tumours including solitary fibrous tumours and gastrointestinal stromal tumours. Proliferating endothelial cells seem to upregulate CD34 expression. Although specificity is low, Anti-CD34 reacts positively with more than 85% of angiosarcoma and Kaposi’s sarcoma.
Cluster of Differentiation 35 (CD35), also known as Erythrocyte Complement Receptor 1 (CR1) or C3b/C4b, is commonly found on erythrocytes, B- and T-cells, monocytes, eosinophils, and neutrophils. It functions to mediate the clearance of opsonized targets. CD35 is a mature B-lymphocyte marker, and Anti-CD35 reacts positively with normal and tumourous follicular dendritic reticulum cells.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC035
Antibody Isotype:
IgG1
GMDN Code:
56976
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Placenta
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 35 (CD35), also known as Erythrocyte Complement Receptor 1 (CR1) or C3b/C4b, is commonly found on erythrocytes, B- and T-cells, monocytes, eosinophils, and neutrophils. It functions to mediate the clearance of opsonized targets. CD35 is a mature B-lymphocyte marker, and Anti-CD35 reacts positively with normal and tumourous follicular dendritic reticulum cells.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC035
Antibody Isotype:
IgG1
GMDN Code:
56976
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Placenta
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 35 (CD35), also known as Erythrocyte Complement Receptor 1 (CR1) or C3b/C4b, is commonly found on erythrocytes, B- and T-cells, monocytes, eosinophils, and neutrophils. It functions to mediate the clearance of opsonized targets. CD35 is a mature B-lymphocyte marker, and Anti-CD35 reacts positively with normal and tumourous follicular dendritic reticulum cells.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC035
Antibody Isotype:
IgG1
GMDN Code:
56976
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil, Placenta
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 35 (CD35), also known as Erythrocyte Complement Receptor 1 (CR1) or C3b/C4b, is commonly found on erythrocytes, B- and T-cells, monocytes, eosinophils, and neutrophils. It functions to mediate the clearance of opsonized targets. CD35 is a mature B-lymphocyte marker, and Anti-CD35 reacts positively with normal and tumourous follicular dendritic reticulum cells.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC038
GMDN Code:
56978
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Plasma Cell Myeloma, Tonsil, Bone Marrow
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 35 (CD35), also known as Erythrocyte Complement Receptor 1 (CR1) or C3b/C4b, is commonly found on erythrocytes, B- and T-cells, monocytes, eosinophils, and neutrophils. It functions to mediate the clearance of opsonized targets. CD35 is a mature B-lymphocyte marker, and Anti-CD35 reacts positively with normal and tumourous follicular dendritic reticulum cells.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC038
GMDN Code:
56978
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Plasma Cell Myeloma, Tonsil, Bone Marrow
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 35 (CD35), also known as Erythrocyte Complement Receptor 1 (CR1) or C3b/C4b, is commonly found on erythrocytes, B- and T-cells, monocytes, eosinophils, and neutrophils. It functions to mediate the clearance of opsonized targets. CD35 is a mature B-lymphocyte marker, and Anti-CD35 reacts positively with normal and tumourous follicular dendritic reticulum cells.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC038
GMDN Code:
56978
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Plasma Cell Myeloma, Tonsil, Bone Marrow
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 3 (CD3) is a T-cell co-receptor expressed by T-cells in thymus, peripheral lymphoid tissue, blood, and bone marrow, as well as activated natural killer cells. CD3 is specifically expressed by T-cells at all stages of development including T-cell lymphomas and leukemias; therefore, it can be used to classify T-cell neoplasms from B-cell and myeloid neoplasms.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC534
Antibody Isotype:
IgG
GMDN Code:
56926
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 3 (CD3) is a T-cell co-receptor expressed by T-cells in thymus, peripheral lymphoid tissue, blood, and bone marrow, as well as activated natural killer cells. CD3 is specifically expressed by T-cells at all stages of development including T-cell lymphomas and leukemias; therefore, it can be used to classify T-cell neoplasms from B-cell and myeloid neoplasms.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC534
Antibody Isotype:
IgG
GMDN Code:
56926
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 3 (CD3) is a T-cell co-receptor expressed by T-cells in thymus, peripheral lymphoid tissue, blood, and bone marrow, as well as activated natural killer cells. CD3 is specifically expressed by T-cells at all stages of development including T-cell lymphomas and leukemias; therefore, it can be used to classify T-cell neoplasms from B-cell and myeloid neoplasms.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC534
Antibody Isotype:
IgG
GMDN Code:
56926
UKCA Status:
UKCA
CE-IVD Status:
RUO
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Synthetic peptide corresponding to amino acids 151-164 of mouse CD3 zeta. _x000D_
Applications:
FC,IP,WB,ICC
Additional Info:
The Armenian hamster antibody H146-968 reacts with CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Synthetic peptide corresponding to amino acids 151-164 of mouse CD3 zeta. _x000D_
Applications:
FC,IP,WB,ICC
Additional Info:
The Armenian hamster antibody H146-968 reacts with CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Synthetic peptide corresponding to amino acids 151-164 of mouse CD3 zeta. _x000D_
Applications:
FC,IP,WB,ICC
Additional Info:
The Armenian hamster antibody H146-968 reacts with CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Immunogen:
Human thymocytes and T lymphocytes.
Applications:
FC,IP,FA
Additional Info:
The antibody MEM-115 recognizes an extracellular epitope in the D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (helper T cells) and also on monocytes, tissue macrophages and granulocytes. It is negative in Western blotting even with non-reduced samples of cell lysates.
Cluster of Differentiation 44 (CD44) is a glycoprotein receptor for hyaluronic acid that plays a fundamental role in cellular adhesion, stromal binding, migration, and cell-cell interactions. Positive staining with Anti-CD44 is implicated in a multitude of different cancer types, including breast, prostatic, renal cell, colonic, hepatocellular, and genitourinary carcinomas, as well as non-Hodgkin's Lymphoma, metastatic melanoma, gastric cancer, and some soft tissue tumours. It has also been demonstrated that there is a positive correlation between tumour progression and increased expression of CD44v, a high molecular weight CD44 isoform that has been described in epithelial cells. Given the expression of CD44 in a wide range of cancers, the most practical application of CD44 immunostaining is its use in discriminating between urothelial transitional cell carcinoma <em>in situ</em> from non-neoplastic changes in the urothelium.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC043
Antibody Isotype:
IgG
GMDN Code:
56984
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Benign Urothelium
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 44 (CD44) is a glycoprotein receptor for hyaluronic acid that plays a fundamental role in cellular adhesion, stromal binding, migration, and cell-cell interactions. Positive staining with Anti-CD44 is implicated in a multitude of different cancer types, including breast, prostatic, renal cell, colonic, hepatocellular, and genitourinary carcinomas, as well as non-Hodgkin's Lymphoma, metastatic melanoma, gastric cancer, and some soft tissue tumours. It has also been demonstrated that there is a positive correlation between tumour progression and increased expression of CD44v, a high molecular weight CD44 isoform that has been described in epithelial cells. Given the expression of CD44 in a wide range of cancers, the most practical application of CD44 immunostaining is its use in discriminating between urothelial transitional cell carcinoma <em>in situ</em> from non-neoplastic changes in the urothelium.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC043
Antibody Isotype:
IgG
GMDN Code:
56984
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Benign Urothelium
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 44 (CD44) is a glycoprotein receptor for hyaluronic acid that plays a fundamental role in cellular adhesion, stromal binding, migration, and cell-cell interactions. Positive staining with Anti-CD44 is implicated in a multitude of different cancer types, including breast, prostatic, renal cell, colonic, hepatocellular, and genitourinary carcinomas, as well as non-Hodgkin's Lymphoma, metastatic melanoma, gastric cancer, and some soft tissue tumours. It has also been demonstrated that there is a positive correlation between tumour progression and increased expression of CD44v, a high molecular weight CD44 isoform that has been described in epithelial cells. Given the expression of CD44 in a wide range of cancers, the most practical application of CD44 immunostaining is its use in discriminating between urothelial transitional cell carcinoma <em>in situ</em> from non-neoplastic changes in the urothelium.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC043
Antibody Isotype:
IgG
GMDN Code:
56984
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Benign Urothelium
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells._x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1_x000D_
Applications:
FC,IP,WB,IHC,ICC
Additional Info:
The rat monoclonal antibody IM7 reacts with CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44._x000D_
CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells._x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1_x000D_
Applications:
FC,IP,WB,IHC,ICC
Additional Info:
The rat monoclonal antibody IM7 reacts with CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44._x000D_
CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells._x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1_x000D_
Applications:
FC,IP,WB,IHC,ICC
Additional Info:
The rat monoclonal antibody IM7 reacts with CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44._x000D_
CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells._x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1_x000D_
Applications:
FC,IP,WB,IHC,ICC
Additional Info:
The rat monoclonal antibody IM7 reacts with CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44._x000D_
CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells._x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1_x000D_
Applications:
FC,IP,WB,IHC,ICC
Additional Info:
The rat monoclonal antibody IM7 reacts with CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44._x000D_
Cluster of Differentiation 44 (CD44) is a glycoprotein receptor for hyaluronic acid that plays a fundamental role in cellular adhesion, stromal binding, migration, and cell-cell interactions. Positive staining with Anti-CD44 is implicated in a multitude of different cancer types, including breast, prostatic, renal cell, colonic, hepatocellular, and genitourinary carcinomas, as well as non-Hodgkin's Lymphoma, metastatic melanoma, gastric cancer, and some soft tissue tumours. It has also been demonstrated that there is a positive correlation between tumour progression and increased expression of CD44v, a high molecular weight CD44 isoform that has been described in epithelial cells. Given the expression of CD44 in a wide range of cancers, the most practical application of CD44 immunostaining is its use in discriminating between urothelial transitional cell carcinoma <em>in situ</em> from non-neoplastic changes in the urothelium.
Cluster of Differentiation 44 (CD44) is a glycoprotein receptor for hyaluronic acid that plays a fundamental role in cellular adhesion, stromal binding, migration, and cell-cell interactions. Positive staining with Anti-CD44 is implicated in a multitude of different cancer types, including breast, prostatic, renal cell, colonic, hepatocellular, and genitourinary carcinomas, as well as non-Hodgkin's Lymphoma, metastatic melanoma, gastric cancer, and some soft tissue tumours. It has also been demonstrated that there is a positive correlation between tumour progression and increased expression of CD44v, a high molecular weight CD44 isoform that has been described in epithelial cells. Given the expression of CD44 in a wide range of cancers, the most practical application of CD44 immunostaining is its use in discriminating between urothelial transitional cell carcinoma <em>in situ</em> from non-neoplastic changes in the urothelium.
Cluster of Differentiation 44 (CD44) is a glycoprotein receptor for hyaluronic acid that plays a fundamental role in cellular adhesion, stromal binding, migration, and cell-cell interactions. Positive staining with Anti-CD44 is implicated in a multitude of different cancer types, including breast, prostatic, renal cell, colonic, hepatocellular, and genitourinary carcinomas, as well as non-Hodgkin's Lymphoma, metastatic melanoma, gastric cancer, and some soft tissue tumours. It has also been demonstrated that there is a positive correlation between tumour progression and increased expression of CD44v, a high molecular weight CD44 isoform that has been described in epithelial cells. Given the expression of CD44 in a wide range of cancers, the most practical application of CD44 immunostaining is its use in discriminating between urothelial transitional cell carcinoma <em>in situ</em> from non-neoplastic changes in the urothelium.
Cluster of Differentiation 45 (CD45), also known as Leukocyte Common Antigen (LCA), is a member of the protein tyrosine phosphatase (PTPase) family that is known to regulate a variety of cellular processes including cell growth, differentiation, the mitotic cycle, and oncogenic transformation. CD45 is expressed in most nucleated cells of hematopoietic origin, and is an essential regulator of T- and B-cell antigen receptor signaling. Anti-CD45 positively stains the majority of lymphoid neoplasms, and is highly indicative of lymphoid origin. However, an absence of CD45 does not rule out lymphoid tumours, as certain types of neoplasms lack CD45, such as Hodgkin's lymphoma, some T-cell lymphomas, and some leukemias.
Cluster of Differentiation 45 (CD45), also known as Leukocyte Common Antigen (LCA), is a member of the protein tyrosine phosphatase (PTPase) family that is known to regulate a variety of cellular processes including cell growth, differentiation, the mitotic cycle, and oncogenic transformation. CD45 is expressed in most nucleated cells of hematopoietic origin, and is an essential regulator of T- and B-cell antigen receptor signaling. Anti-CD45 positively stains the majority of lymphoid neoplasms, and is highly indicative of lymphoid origin. However, an absence of CD45 does not rule out lymphoid tumours, as certain types of neoplasms lack CD45, such as Hodgkin's lymphoma, some T-cell lymphomas, and some leukemias.
Cluster of Differentiation 45 (CD45), also known as Leukocyte Common Antigen (LCA), is a member of the protein tyrosine phosphatase (PTPase) family that is known to regulate a variety of cellular processes including cell growth, differentiation, the mitotic cycle, and oncogenic transformation. CD45 is expressed in most nucleated cells of hematopoietic origin, and is an essential regulator of T- and B-cell antigen receptor signaling. Anti-CD45 positively stains the majority of lymphoid neoplasms, and is highly indicative of lymphoid origin. However, an absence of CD45 does not rule out lymphoid tumours, as certain types of neoplasms lack CD45, such as Hodgkin's lymphoma, some T-cell lymphomas, and some leukemias.
CD45R, also known as MB1, is an isoform of CD45 that is a member of the protein tyrosine phosphatase (PTPase) family. CD45R is expressed specifically on the surface of hematopoietic cells, and has demonstrated function as a regulator of the antigen and cytokine receptor signaling of B- and T-cells. Given that the antigen is located in the membrane of all B-cells, with the exception of plasma cells and some mature T-cells, Anti-CD45R exhibits specific reactivity with most B-lymphocytes. The use of Anti-CD45R is primarily useful in distinguishing B-cell lymphomas from T-cell lymphomas, with specific reactivity to follicle center cells, mantle cells, some medullary thymocytes, and 80% of B-cell lymphomas.
CD45R, also known as MB1, is an isoform of CD45 that is a member of the protein tyrosine phosphatase (PTPase) family. CD45R is expressed specifically on the surface of hematopoietic cells, and has demonstrated function as a regulator of the antigen and cytokine receptor signaling of B- and T-cells. Given that the antigen is located in the membrane of all B-cells, with the exception of plasma cells and some mature T-cells, Anti-CD45R exhibits specific reactivity with most B-lymphocytes. The use of Anti-CD45R is primarily useful in distinguishing B-cell lymphomas from T-cell lymphomas, with specific reactivity to follicle center cells, mantle cells, some medullary thymocytes, and 80% of B-cell lymphomas.
CD45R, also known as MB1, is an isoform of CD45 that is a member of the protein tyrosine phosphatase (PTPase) family. CD45R is expressed specifically on the surface of hematopoietic cells, and has demonstrated function as a regulator of the antigen and cytokine receptor signaling of B- and T-cells. Given that the antigen is located in the membrane of all B-cells, with the exception of plasma cells and some mature T-cells, Anti-CD45R exhibits specific reactivity with most B-lymphocytes. The use of Anti-CD45R is primarily useful in distinguishing B-cell lymphomas from T-cell lymphomas, with specific reactivity to follicle center cells, mantle cells, some medullary thymocytes, and 80% of B-cell lymphomas.
CD45RO is an isoform of CD45 which is expressed in thymocytes, activated T-cells, and subpopulations of resting T-cells. It is a useful marker for T-cell tumours, as Anti-CD45RO demonstrates no reactivity with B-cells. Specifically, CD45RO is implicated in a number of T-cell lymphomas including angioimmunoblastic, lymphoblastic, peripheral, and subcutaneous panniculitis-like T-cell lymphomas.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC537
Antibody Isotype:
IgG2a
GMDN Code:
56992
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
CD45RO is an isoform of CD45 which is expressed in thymocytes, activated T-cells, and subpopulations of resting T-cells. It is a useful marker for T-cell tumours, as Anti-CD45RO demonstrates no reactivity with B-cells. Specifically, CD45RO is implicated in a number of T-cell lymphomas including angioimmunoblastic, lymphoblastic, peripheral, and subcutaneous panniculitis-like T-cell lymphomas.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC537
Antibody Isotype:
IgG2a
GMDN Code:
56992
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
CD45RO is an isoform of CD45 which is expressed in thymocytes, activated T-cells, and subpopulations of resting T-cells. It is a useful marker for T-cell tumours, as Anti-CD45RO demonstrates no reactivity with B-cells. Specifically, CD45RO is implicated in a number of T-cell lymphomas including angioimmunoblastic, lymphoblastic, peripheral, and subcutaneous panniculitis-like T-cell lymphomas.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC537
Antibody Isotype:
IgG2a
GMDN Code:
56992
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Tonsil
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 4 (CD4) is a membrane glycoprotein expressed in T helper cells, monocytes, macrophages, granulocytes, and dendritic cells, and is a receptor of human immunodeficiency virus (HIV). CD4 staining is used for identifying lymphoproliferative disorders. Since the majority of peripheral T-cell lymphomas arise from the T helper cell subset, CD4 expression can be found in most forms of T-cell lymphomas as well as anaplastic large T-cell lymphomas and mycosis fungoides. Since CD4 may be aberrantly expressed in neoplastic T-lymphocytes, a panel of markers may be used to identify such tumours. CD4(+) CD25(+) T-cells are reported to exert immunosuppression, which is commonly observed in various types of cancers, including non-small cell lung cancer and cancers of the breast, prostate, and ovary.
Cluster of Differentiation 4 (CD4) is a membrane glycoprotein expressed in T helper cells, monocytes, macrophages, granulocytes, and dendritic cells, and is a receptor of human immunodeficiency virus (HIV). CD4 staining is used for identifying lymphoproliferative disorders. Since the majority of peripheral T-cell lymphomas arise from the T helper cell subset, CD4 expression can be found in most forms of T-cell lymphomas as well as anaplastic large T-cell lymphomas and mycosis fungoides. Since CD4 may be aberrantly expressed in neoplastic T-lymphocytes, a panel of markers may be used to identify such tumours. CD4(+) CD25(+) T-cells are reported to exert immunosuppression, which is commonly observed in various types of cancers, including non-small cell lung cancer and cancers of the breast, prostate, and ovary.
Cluster of Differentiation 4 (CD4) is a membrane glycoprotein expressed in T helper cells, monocytes, macrophages, granulocytes, and dendritic cells, and is a receptor of human immunodeficiency virus (HIV). CD4 staining is used for identifying lymphoproliferative disorders. Since the majority of peripheral T-cell lymphomas arise from the T helper cell subset, CD4 expression can be found in most forms of T-cell lymphomas as well as anaplastic large T-cell lymphomas and mycosis fungoides. Since CD4 may be aberrantly expressed in neoplastic T-lymphocytes, a panel of markers may be used to identify such tumours. CD4(+) CD25(+) T-cells are reported to exert immunosuppression, which is commonly observed in various types of cancers, including non-small cell lung cancer and cancers of the breast, prostate, and ovary.
CD54 (ICAM-1) is a member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Immunogen:
Mouse NS-1 cells_x000D_
Applications:
FC,IP,WB,IHC,ICC,FA
Additional Info:
The rat monoclonal antibody YN1/1.7.4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation. _x000D_
CD54 (ICAM-1) is a member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Mouse NS-1 cells_x000D_
Applications:
FC,IP,WB,IHC,ICC,FA
Additional Info:
The rat monoclonal antibody YN1/1.7.4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation. _x000D_
CD54 (ICAM-1) is a member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation. _x000D_
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Mouse NS-1 cells_x000D_
Applications:
FC,IP,WB,IHC,ICC,FA
Additional Info:
The rat monoclonal antibody YN1/1.7.4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation. _x000D_
Cluster of Differentiation 56 (CD56), also known as Neural-Cell Adhesion Molecule (NCAM), is a glycoprotein involved in synaptic plasticity, cell-cell adhesion, neurite outgrowth, learning, and memory. NCAM is expressed in normal neurons, glia, natural killer cells, activated T-cells, brain and cerebellum, neuroendocrine tissues, and skeletal muscle. Anti-CD56 recognizes a number of tumours including myeloma, myeloid leukemia, natural killer/T-cell lymphomas, neuroendocrine tumours, pancreatic acinar-cell carcinoma, pheochromocytoma, and Wilm's tumour. CD56 is detectable in neoplasms that are neuroectodermally-derived, such as retinoblastoma, medulloblastomas, astrocytomas, small cell carcinomas, and neuroblastomas. It has also been linked to rhabdomyosarcoma, a tumour that is mesodermally-derived.
Cluster of Differentiation 56 (CD56), also known as Neural-Cell Adhesion Molecule (NCAM), is a glycoprotein involved in synaptic plasticity, cell-cell adhesion, neurite outgrowth, learning, and memory. NCAM is expressed in normal neurons, glia, natural killer cells, activated T-cells, brain and cerebellum, neuroendocrine tissues, and skeletal muscle. Anti-CD56 recognizes a number of tumours including myeloma, myeloid leukemia, natural killer/T-cell lymphomas, neuroendocrine tumours, pancreatic acinar-cell carcinoma, pheochromocytoma, and Wilm's tumour. CD56 is detectable in neoplasms that are neuroectodermally-derived, such as retinoblastoma, medulloblastomas, astrocytomas, small cell carcinomas, and neuroblastomas. It has also been linked to rhabdomyosarcoma, a tumour that is mesodermally-derived.
Cluster of Differentiation 56 (CD56), also known as Neural-Cell Adhesion Molecule (NCAM), is a glycoprotein involved in synaptic plasticity, cell-cell adhesion, neurite outgrowth, learning, and memory. NCAM is expressed in normal neurons, glia, natural killer cells, activated T-cells, brain and cerebellum, neuroendocrine tissues, and skeletal muscle. Anti-CD56 recognizes a number of tumours including myeloma, myeloid leukemia, natural killer/T-cell lymphomas, neuroendocrine tumours, pancreatic acinar-cell carcinoma, pheochromocytoma, and Wilm's tumour. CD56 is detectable in neoplasms that are neuroectodermally-derived, such as retinoblastoma, medulloblastomas, astrocytomas, small cell carcinomas, and neuroblastomas. It has also been linked to rhabdomyosarcoma, a tumour that is mesodermally-derived.
Cluster of Differentiation 57 (CD57), also known as NK-1, is an antigen detectable in natural killer cells, some T-lymphocytes and normal peripheral blood mononuclear cells, myeloid cells, and a variety of polypeptides, lipids, and chondroitin sulfate proteoglycans. CD57 is indicated as a marker for tumours of neuroendocrine origin, including pheochromocytomas, paragangliomas, medulloblastomas, and carcinoid tumour, as well as various neural tumours including neuromas, neurofibromas, schwannomas, and granular cell tumours. CD57 is also detectable in ganglioneuroma and prostate carcinoma. Anti-CD57 is used to distinguish nodular lymphocyte-predominant Hodgkin's lymphoma from T-cell/histiocyte-rich large B-cell lymphoma, nodular sclerosis Hodgkin's disease, and follicular lymphoma.
Cluster of Differentiation 57 (CD57), also known as NK-1, is an antigen detectable in natural killer cells, some T-lymphocytes and normal peripheral blood mononuclear cells, myeloid cells, and a variety of polypeptides, lipids, and chondroitin sulfate proteoglycans. CD57 is indicated as a marker for tumours of neuroendocrine origin, including pheochromocytomas, paragangliomas, medulloblastomas, and carcinoid tumour, as well as various neural tumours including neuromas, neurofibromas, schwannomas, and granular cell tumours. CD57 is also detectable in ganglioneuroma and prostate carcinoma. Anti-CD57 is used to distinguish nodular lymphocyte-predominant Hodgkin's lymphoma from T-cell/histiocyte-rich large B-cell lymphoma, nodular sclerosis Hodgkin's disease, and follicular lymphoma.
Cluster of Differentiation 57 (CD57), also known as NK-1, is an antigen detectable in natural killer cells, some T-lymphocytes and normal peripheral blood mononuclear cells, myeloid cells, and a variety of polypeptides, lipids, and chondroitin sulfate proteoglycans. CD57 is indicated as a marker for tumours of neuroendocrine origin, including pheochromocytomas, paragangliomas, medulloblastomas, and carcinoid tumour, as well as various neural tumours including neuromas, neurofibromas, schwannomas, and granular cell tumours. CD57 is also detectable in ganglioneuroma and prostate carcinoma. Anti-CD57 is used to distinguish nodular lymphocyte-predominant Hodgkin's lymphoma from T-cell/histiocyte-rich large B-cell lymphoma, nodular sclerosis Hodgkin's disease, and follicular lymphoma.
Cluster of Differentiation 5 (CD5) is expressed in high levels on the surface of T-cells, while the expression levels and role of CD5 in B-cells is not well documented. As a part of a diagnostic panel, its utility lies predominantly as a marker for T-cells, with over 70% of T-cell neoplasms expressing CD5. In particular, it is correlated with chronic lymphocytic leukemia and small lymphocytic lymphomas, mantle cell lymphoma, as well as a subset of diffuse large B-cell lymphomas. CD5 demonstrates positive expression in thymic carcinomas, and is not as sensitive as CD3. CD5 also has value as a prognostic indicator, as it is associated with poor prognosis in acute T-cell lymphoblastic leukemia.
Cluster of Differentiation 5 (CD5) is expressed in high levels on the surface of T-cells, while the expression levels and role of CD5 in B-cells is not well documented. As a part of a diagnostic panel, its utility lies predominantly as a marker for T-cells, with over 70% of T-cell neoplasms expressing CD5. In particular, it is correlated with chronic lymphocytic leukemia and small lymphocytic lymphomas, mantle cell lymphoma, as well as a subset of diffuse large B-cell lymphomas. CD5 demonstrates positive expression in thymic carcinomas, and is not as sensitive as CD3. CD5 also has value as a prognostic indicator, as it is associated with poor prognosis in acute T-cell lymphoblastic leukemia.
Cluster of Differentiation 5 (CD5) is expressed in high levels on the surface of T-cells, while the expression levels and role of CD5 in B-cells is not well documented. As a part of a diagnostic panel, its utility lies predominantly as a marker for T-cells, with over 70% of T-cell neoplasms expressing CD5. In particular, it is correlated with chronic lymphocytic leukemia and small lymphocytic lymphomas, mantle cell lymphoma, as well as a subset of diffuse large B-cell lymphomas. CD5 demonstrates positive expression in thymic carcinomas, and is not as sensitive as CD3. CD5 also has value as a prognostic indicator, as it is associated with poor prognosis in acute T-cell lymphoblastic leukemia.
Cluster of Differentiation 61 (CD61), also known as Glycoprotein IIIa or GPIIIa, is an antigen expressed on megakaryocytes, platelets, myeloid cells, monocytes, endothelial cells, smooth muscle cells, and macrophages. It is involved in platelet aggregation and acts as a receptor for fibrinogen, fibronectin, von Willebrand factor, and vitronectin. Anti-CD61 is used for identifying megakaryocytopoiesis, as seen in megakaryoblastic leukemias, myelodysplastic disorders, and acute myeloid leukemias. CD61 is also indicated as a marker for platelet adhesion in advanced atherosclerosis and has been reported in the identification of fat embolism in pulmonary tissue.
Cluster of Differentiation 61 (CD61), also known as Glycoprotein IIIa or GPIIIa, is an antigen expressed on megakaryocytes, platelets, myeloid cells, monocytes, endothelial cells, smooth muscle cells, and macrophages. It is involved in platelet aggregation and acts as a receptor for fibrinogen, fibronectin, von Willebrand factor, and vitronectin. Anti-CD61 is used for identifying megakaryocytopoiesis, as seen in megakaryoblastic leukemias, myelodysplastic disorders, and acute myeloid leukemias. CD61 is also indicated as a marker for platelet adhesion in advanced atherosclerosis and has been reported in the identification of fat embolism in pulmonary tissue.
Cluster of Differentiation 61 (CD61), also known as Glycoprotein IIIa or GPIIIa, is an antigen expressed on megakaryocytes, platelets, myeloid cells, monocytes, endothelial cells, smooth muscle cells, and macrophages. It is involved in platelet aggregation and acts as a receptor for fibrinogen, fibronectin, von Willebrand factor, and vitronectin. Anti-CD61 is used for identifying megakaryocytopoiesis, as seen in megakaryoblastic leukemias, myelodysplastic disorders, and acute myeloid leukemias. CD61 is also indicated as a marker for platelet adhesion in advanced atherosclerosis and has been reported in the identification of fat embolism in pulmonary tissue.
Cluster of Differentiation 63 (CD63) is a lysosomal membrane glycoprotein identified as a platelet activation molecule. CD63 localizes to the membrane and cytoplasm of many cell types including lymphoid, myeloid, and endothelial cells. CD63 is a useful marker for malignant melanoma, and for distinguishing between renal oncocytoma and eosinophilic renal cell carcinoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC540
Antibody Isotype:
IgG1, kappa
GMDN Code:
62544
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Melanoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 63 (CD63) is a lysosomal membrane glycoprotein identified as a platelet activation molecule. CD63 localizes to the membrane and cytoplasm of many cell types including lymphoid, myeloid, and endothelial cells. CD63 is a useful marker for malignant melanoma, and for distinguishing between renal oncocytoma and eosinophilic renal cell carcinoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Concentrate
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC540
Antibody Isotype:
IgG1, kappa
GMDN Code:
62544
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Melanoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
Cluster of Differentiation 63 (CD63) is a lysosomal membrane glycoprotein identified as a platelet activation molecule. CD63 localizes to the membrane and cytoplasm of many cell types including lymphoid, myeloid, and endothelial cells. CD63 is a useful marker for malignant melanoma, and for distinguishing between renal oncocytoma and eosinophilic renal cell carcinoma.
Product Type:
Primary Antibody
Antibody Type:
Monoclonal
Format:
Predilute
Storage Temp:
2-8 degrees Celsius
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant Protein
Applications:
IHC
Clone number:
IHC540
Antibody Isotype:
IgG1, kappa
GMDN Code:
62544
UKCA Status:
UKCA
CE-IVD Status:
IVDD
Positive Control:
Melanoma
Purification:
Affinity Purification
Buffer:
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
The CD66 molecules are 180-200 kDa glycoproteins of carcinoembryonic antigen family. They are present on all blood granulocytes and some tissue macrophages, but are absent from other hematopoietic cells. The expression of CD66 increases significantly on granulocytes upon their activation. CD66a (BGP1, CEACAM1) and CD66d (CGM1, CEACAM3), as well as CEACAM2 and 4, are transmembrane proteins, whereas CD66c (CEAL, NCA, CEACAM6) and CD66e (CEA, CEACAM5), as well as CEACAM7 and 8, are anchored to the plasma membrane by C-terminal glycosylphosphatidylinositol (GPI) lipid moiety.
Product Type:
Antibodies Primary
Antibody Type:
monoclonal
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Immunogen:
Human granulocytes
Applications:
FC,IP,WB,IHC,ICC
Additional Info:
The mouse monoclonal antibody CLB-gran/10 (IH4Fc) detects human CD66acde antigen, a granulocyte marker, especially after cell stimulation. The antibody does not cross-react with normal human peripheral B cells, T cells, monocytes and platelets. Weak reactivity has been observed with malignant cells of pacients with B cell-derived CLL. In immunohistochemistry the antibody reacts with some tissue macrophages and carcinoma-expressed CEA. <br>_x000D_ <b>HLDA IV; WS Code M38</b>
Cluster of Differentiation 68 (CD68) is a heavily glycosylated transmembrane antigen that is detected in lysosomes, tissue macrophages, Langerhans cells, dendritic cells, monocytes, Kupffer cells, osteoclasts, and granulocytes. Anti-CD68 may be useful in identifying myelomonocytic and histiocytic tumours, and for differentiating between malignant fibrous histiocytoma and other pleomorphic sarcomas. However, other lysosome-rich cells may also stain, since Anti-CD68 detects a formalin-resistant epitope that may be associated with lysosomal granules.
Cluster of Differentiation 68 (CD68) is a heavily glycosylated transmembrane antigen that is detected in lysosomes, tissue macrophages, Langerhans cells, dendritic cells, monocytes, Kupffer cells, osteoclasts, and granulocytes. Anti-CD68 may be useful in identifying myelomonocytic and histiocytic tumours, and for differentiating between malignant fibrous histiocytoma and other pleomorphic sarcomas. However, other lysosome-rich cells may also stain, since Anti-CD68 detects a formalin-resistant epitope that may be associated with lysosomal granules.
Tris Buffer pH7.6 with BSA, and sodium azide as preservative
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