Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
Purple dye: If Cy5 or similar dye is used as one of the fluorophores, Purple passive reference dye might interfere with the signal. A version without passive reference or with ROX reference dye is available
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
Purple dye: If Cy5 or similar dye is used as one of the fluorophores, Purple passive reference dye might interfere with the signal. A version without passive reference or with ROX reference dye is available
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
Purple dye: If Cy5 or similar dye is used as one of the fluorophores, Purple passive reference dye might interfere with the signal. A version without passive reference or with ROX reference dye is available
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
Purple dye: If Cy5 or similar dye is used as one of the fluorophores, Purple passive reference dye might interfere with the signal. A version without passive reference or with ROX reference dye is available
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
ROX dye: If ROX, Texas Red or similar dye is used as one of the fluorophores, ROX passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available.
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
ROX dye: If ROX, Texas Red or similar dye is used as one of the fluorophores, ROX passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available.
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
ROX dye: If ROX, Texas Red or similar dye is used as one of the fluorophores, ROX passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available.
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
ROX dye: If ROX, Texas Red or similar dye is used as one of the fluorophores, ROX passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available.
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for singleplex and multiplex endpoint PCR assays. It contains a hot-start Taq DNA polymerase HOT FIREPol® and allows amplification of up to 5 kb DNA templates. The mix has two versions - regular and Ready-to-Load mix which includes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
sensitive detection of up to 18 targets per reaction suitable for templates up to 5 kb increased sensitivity and yield reduced primer dimer formation Ready-to-Load mix available reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase 5x Multiplex Buffer with 10 mM MgCl2 (1x PCR solution 2 mM MgCl2) dNTPs BSA Compound that increases sample density for direct loading (loading dyes not included)
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for singleplex and multiplex endpoint PCR assays. It contains a hot-start Taq DNA polymerase HOT FIREPol® and allows amplification of up to 5 kb DNA templates. The mix has two versions - regular and Ready-to-Load mix which includes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
sensitive detection of up to 18 targets per reaction suitable for templates up to 5 kb increased sensitivity and yield reduced primer dimer formation Ready-to-Load mix available reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase 5x Multiplex Buffer with 10 mM MgCl2 (1x PCR solution 2 mM MgCl2) dNTPs BSA Compound that increases sample density for direct loading (loading dyes not included)
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for singleplex and multiplex endpoint PCR assays. It contains a hot-start Taq DNA polymerase HOT FIREPol® and allows amplification of up to 5 kb DNA templates. The mix has two versions - regular and Ready-to-Load mix which includes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
sensitive detection of up to 18 targets per reaction suitable for templates up to 5 kb increased sensitivity and yield reduced primer dimer formation Ready-to-Load mix available reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase 5x Multiplex Buffer with 10 mM MgCl2 (1x PCR solution 2 mM MgCl2) dNTPs BSA Compound that increases sample density for direct loading (loading dyes not included)
Hot-start Master Mix designed to provide highly specific high-yield amplification of GC-rich templates.
Background Info:
A ready-to-use 5X-concentrated solution for endpoint PCR assays, optimised for GC-rich templates. It contains a hot-start Taq DNA polymerase HOT FIREPol® and allows amplification of up to 5 kb DNA templates. Separate tubes of 25 mM MgCl? and an additive for difficult templates (100% DMSO) are included with the mix.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
excellent amplification with templates up to 79% GC content suitable for templates up to 5 kb vials of 100% DMSO and 25 mM MgCl? enable flexibility in reaction optimization reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x HOT FIREPol® GC Buffer with 7.5 mM MgCl2: 1x PCR solution 1.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP Bovine serum albumin (BSA) to enhance PCR reaction In separate vials: 100% DMSO is included in the kit in a separate vial. DMSO is recommended as a PCR additive for templates with high GC content. In some cases, DMSO is also required to relax secondary structures. 25 mM MgCl2 for further optimization
Vials included in the kit:
HOT FIREPol® GC Master Mix 1x20 ml | 5000 rxn 25 mM MgCl2 20x0.5 ml DMSO 100% 1x10 ml
Hot-start Master Mix designed to provide highly specific high-yield amplification of GC-rich templates.
Background Info:
A ready-to-use 5X-concentrated solution for endpoint PCR assays, optimised for GC-rich templates. It contains a hot-start Taq DNA polymerase HOT FIREPol® and allows amplification of up to 5 kb DNA templates. Separate tubes of 25 mM MgCl? and an additive for difficult templates (100% DMSO) are included with the mix.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
excellent amplification with templates up to 79% GC content suitable for templates up to 5 kb vials of 100% DMSO and 25 mM MgCl? enable flexibility in reaction optimization reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x HOT FIREPol® GC Buffer with 7.5 mM MgCl2: 1x PCR solution 1.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP Bovine serum albumin (BSA) to enhance PCR reaction In separate vials: 100% DMSO is included in the kit in a separate vial. DMSO is recommended as a PCR additive for templates with high GC content. In some cases, DMSO is also required to relax secondary structures. 25 mM MgCl2 for further optimization
Vials included in the kit:
HOT FIREPol® GC Master Mix 1x0.1 ml | 25 rxn 25 mM MgCl2 1x0.1 ml DMSO 100% 1x0.1 ml
Hot-start Master Mix designed to provide highly specific high-yield amplification of GC-rich templates.
Background Info:
A ready-to-use 5X-concentrated solution for endpoint PCR assays, optimised for GC-rich templates. It contains a hot-start Taq DNA polymerase HOT FIREPol® and allows amplification of up to 5 kb DNA templates. Separate tubes of 25 mM MgCl? and an additive for difficult templates (100% DMSO) are included with the mix.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
excellent amplification with templates up to 79% GC content suitable for templates up to 5 kb vials of 100% DMSO and 25 mM MgCl? enable flexibility in reaction optimization reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x HOT FIREPol® GC Buffer with 7.5 mM MgCl2: 1x PCR solution 1.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP Bovine serum albumin (BSA) to enhance PCR reaction In separate vials: 100% DMSO is included in the kit in a separate vial. DMSO is recommended as a PCR additive for templates with high GC content. In some cases, DMSO is also required to relax secondary structures. 25 mM MgCl2 for further optimization
Vials included in the kit:
HOT FIREPol® GC Master Mix 1x1 ml | 250 rxn 25 mM MgCl2 1x0.5 ml DMSO 100% 1x0.5 ml
Robust and reliable hot-start Master Mix with higher fidelity and longer amplification range for more demanding reactions.
Background Info:
A ready-to-use 5X-concentrated solution for more demanding endpoint PCR assays. In addition to the hot-start Taq polymerase HOT FIREPol® this master mix contains a proofreading enzyme which offers enhanced fidelity and performance. The mix has regular and Ready-to-Load version and different MgCl? options for easier optimization.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
increased yield, sensitivity and specificity up to 5x higher fidelity suitable for templates up to 5 kb reduced primer dimer formation Ready-to-Load mix available reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start Proofreading enzyme: to enhance fidelity 5x Blend Master Mix Buffer with 12. 5 mM MgCl2 1x PCR solution 1.5 mM MgCl2 1 mM dNTPs of each: 1x PCR solution 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP Bovine serum albumin (BSA) to enhance PCR reaction
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