VDAC proteins are porin-type, beta-barrel diffusion pores, Prominently localized in the outer mitochondrial membrane and involved in metabolite exchange,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
To be added when available. Antibody released in May 2023.
Special application note:
Cellular [compartment marker] of mitochondrial outer membrane.DyLight 488 Amax = 493 nm, Emax = 519 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
VDAC proteins are porin-type, beta-barrel diffusion pores, Prominently localized in the outer mitochondrial membrane and involved in metabolite exchange.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
To be added when available. Antibody released in May 2023.
Special application note:
Cellular [compartment marker] of mitochondrial outer membrane.DyLight 594 has Amax = 593 nm, Emax = 618 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
VDAC proteins are porin-type, beta-barrel diffusion pores, Prominently localized in the outer mitochondrial membrane and involved in metabolite exchange.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
To be added when available. Antibody released in May 2023.
Special application note:
Cellular [compartment marker] of mitochondrial outer membrane.DyLight 650 has Amax = 652 nm, Emax = 672 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
VDAC proteins are porin-type, beta-barrel diffusion pores. Prominently localized in the outer mitochondrial membrane and involved in metabolite exchange.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 g.Immunolocalization method description and images are available hereBlue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010
Application Details:
1 : 500 (IL), 1 : 5000, 2-30 g protein/lane (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP.
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana UniProt: Q39258-1, TAIR: At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).
Applications:
Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.Immunostaining protocol using V-ATPase antibodies can be found here.
Saccharomyces cerevisiaereplication protein A (RPA), also known as replication factor A (RFA) is a single-stranded DNA-binding protein that is required for multiple processes in eukaryotic DNA metabolism. Those processes include DNA replication, DNA repair, and recombination. Homologues to RPA have been identified in all eukaryotic organisms examined. RPA is heterotrimeric protein composed of subunits of approximately 70, 30, and 14 kDa. Members of this family bind nonspecifically to single-stranded DNA and interact with and/or modify the activities of multiple proteins. Alternative names: Replication protein A 69 kDa DNA-binding subunit, Single-stranded DNA-binding protein, DNA-binding protein BUF2, replication protein A 36 kDa subunit, DNA-binding protein BUF1 antibody
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Saccharomyces cerevisiae
Expected Species:
Saccharomyces cerevisiae
Immunogen:
RPA from Saccharomyces cerevisiae consisting of three subunits RFA1 (70 kDa), RFA2 (30 kDa) and RFA3 (14 kDa); overexpressed in E.coli and purified by chromatography; no affinity tags were added to any of three subunits
Applications:
Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Western blot (WB)
Antibody was also successfully used in ChIP application Holstein et al. (2014).Load of 1 ng of the protein will allow to visualize two subunits of RPA, while load of 5 ng will allow to visualize all three subunits in Western blot technique.
Application Details:
ChIP, 1 : 20 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
70 + 30 + 14 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Scherzer et al (2022). Recruitment of Scc2/4 to double-strand breaks depends on ?H2A and DNA end resection. Life Sci Alliance. 2022 Jan 27;5(5):e202101244. doi: 10.26508/lsa.202101244. PMID: 35086935; PMCID: PMC8807874.Minchell et al. (2020). Cohesin Causes Replicative DNA Damage by Trapping DNA Topological Stress. Mol Cell . 2020 Mar 29;S1097-2765(20)30161-1. doi: 10.1016/j.molcel.2020.03.013. He et. al (2019). KEOPS complex promotes homologous recombination via DNA resection. Nucleic Acids Res. 2019 Apr 2. pii: gkz228. doi: 10.1093/nar/gkz228. Jakobsen et al. (2019). Minimal Resection Takes Place during Break-Induced Replication Repair of Collapsed Replication Forks and Is Controlled by Strand Invasion. Cell Rep. 2019 Jan 22;26(4):836-844.e3. doi: 10.1016/j.celrep.2018.12.108. (used AS07 214-100, which is a larger size unit of AS07 214)Deshpande et al. (2017). Structural Basis of Mec1-Ddc2-RPA Assembly and Activation on Single-Stranded DNA at Sites of Damage. Mol Cell. 2017 Oct 19;68(2):431-445.e5. doi: 10.1016/j.molcel.2017.09.019.
FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria as well as in chloroplasts. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This antibody can be used as a loading control antibody in cyanobacteria. Immunofluorescence has been done by labelling Synechococcus elongatus cells at 30 C for 2 hours with FtsZ antibodies diluted to 1: 500 in blocking buffer. Detection images can be found in Kabeya et al (2010).This product can be sold containing ProClin if requested.
Kurmayer et al. (2020). Chemically labeled toxins or bioactive peptides show a heterogeneous intracellular distribution and low spatial overlap with autofluorescence in bloom-forming cyanobacteria. Sci Rep. 2020 Feb 17;10(1):2781. doi: 10.1038/s41598-020-59381-w. (Immunofluorescence)Zhan et al. (2018). Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus. J Vis Exp. 2018 Nov 6;(141). doi: 10.3791/58603.MacCready et al. (2016). Robust Min-System Oscillation in the Presence of Internal Photosynthetic Membranes in Cyanobacteria. Molecular Microbiology November 5 2016. doi: 10.1111/mmi.13571Probst et al. (2014). Biology of a widespread uncultivated archaeon that contributes to carbon fixation in the subsurface. Nat Commun. 2014 Nov 26;5:5497. doi: 10.1038/ncomms6497.Miyagishima et al. (2014). DipM is required for peptidoglycan hydrolysis during chloroplast division. BMC Plant Biol. 2014 Mar 6;14(1):57. (immunofluorescence)Plominsky et al. (2013). Dinitrogen Fixation Is Restricted to the Terminal Heterocysts in the Invasive Cyanobacterium Cylindrospermopsis raciborskii CS-505. PLOS ONE, Open Access.Kabeya et al (2010). The YlmG protein has a conserved function related to the distribution of nucleoids in chloroplasts and cyanobacteria. BMC Plant Biology 10:57.
Special application note:
To detect E.coli FtsZ protein we recommend a following product: AS10 715 | anti-FtsZ procaryotic cell division GTPase (bacterial), rabbit antibodyTo detect FtsZ protein in higher plants following antibodies are recommended: AS09 413 | Anti-FtsZ1 and 2 | Plant cell division protein FtsZ1 and FtsZ2, rabbit antibodiesAS13 2651 | Anti-FtsZ2 | Plant cell division protein ftsZ2, rabbit antibodies
Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
RbcS subunit is not detected by this antibodyThis product can be sold containing proclin if requested
Application Details:
1 : 10 000-1 : 20 000 on 0,5-10 ug total cellular protein/lane and standard (WB), 1 : 500-1:1000 (IL)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
53-55 | 53-55 kDa
Not reactive in:
Chlamydomonas reinhardtii (immulolocalization)
Selected references:
Mihara et al. (2019). Thioredoxin targets are regulated in heterocysts of cyanobacterium Anabaena sp. PCC 7120 in a light-independent manner. J Exp Bot. 2019 Dec 21. pii: erz561. doi: 10.1093/jxb/erz561.Sedaghatmehr et al. (2019). A regulatory role of autophagy for resetting the memory of heat stress in plants. Plant Cell Environ. 2019 Mar;42(3):1054-1064. doi: 10.1111/pce.13426.Rohnke et al. (2018). RcaE-Dependent Regulation of Carboxysome Structural Proteins Has a Central Role in Environmental Determination of Carboxysome Morphology and Abundance in Fremyella diplosiphon. Mol Biol and Physiol. Vol. 3, Issue 1. DOI: 10.1128/mSphere.00617-17Zhang et al. (2017). Composition of photosynthetic pigments and photosynthetic characteristics in green and yellow sectors of the variegated Aucuba japonica ‘Variegata’ leaves. Flora, Vol 240, March 2018, Pages 25–33.Wang et al. (2017). Re-creation of a Key Step in the Evolutionary Switch from C3 to C4 Leaf Anatomy. Curr Biol. 2017 Nov 6;27(21):3278-3287.e6. doi: 10.1016/j.cub.2017.09.040.Wen and Zhang (2014). Salsola laricifolia, another C 3 –C 4 intermediate species in tribe Salsoleae s.l. (Chenopodiaceae). Photosynth Res. 2014 Sep 17. (immunolocalization)Feifei et al. (2014). Comparison of Leaf Proteomes of Cassava (Manihot esculenta Crantz) Cultivar NZ199 Diploid and Autotetraploid Genotypes. PLoS One. 2014 Apr 11;9(4):e85991. doi: 10.1371/journal.pone.0085991. eCollection 2014.
Copper is an essential element for function of chloroplasts. It is a co-factor for superoxide dismutase (SOD) and for plastocyanin. Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets. In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site. The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Glycne max, Hordeum vulgare, Solanum tuberosum Species of your interest not listed? Contact us
Immunogen:
The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5, fused to His-tag. Overexpressed using pET28a vector in Bl21 E. coli (Cd+) and purified using His-column. After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Speth et al. (2013). RACK1 scaffold proteins influence miRNA abundance in Arabidopsis. Plant J. Aug 13.Abdel-Ghany et al (2005). .AtCCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7. FEBS Lett. 11:2307-12.
Phytochrome A (PhyA) is the primary photoreceptor mediating various responses to far-red (FR) light in plants. Alternative protein names: FAR RED ELONGATED HYPOCOTYL 2, FAR RED ELONGATED 1, ELONGATED HYPOCOTYL 8.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Brassica rapa, Cardamine hirsuta, Dacucus carota, Lathyrus sativus, Fragaria ananassa, Glycine max, Gossyoium hirsutum, Hordeum vulgare, Lotus corniculatus, Medicago truncatula, Nicotiana benthamiana (PhyA1), Nicotiana tabacum, Pisum sativum, Populus balsamifera, Ricinus communis, Solanum lycopersicumSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from conserved plant PhyA protein sequences including Arabidopsis thaliana UniProt:P14712, TAIR:At1g59070; peptide sequence is not present in other plant phytochrome forms (B-E)
Careful sample collection is adviced to assure the best results with this antibody
Application Details:
1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
124 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Schwenk et al. (2021) Uncovering a novel function of the CCR4-NOT complex in phytochrome A-mediated light signalling in plants. Elife. 2021 Mar 30;10:e63697. doi: 10.7554/eLife.63697. PMID: 33783355; PMCID: PMC8009681.Schenk et al. (2021) Light-induced degradation of SPA2 via its N-terminal kinase domain is required for photomorphogenesis, Plant Physiology, 2021;, kiab156, https://doi.org/10.1093/plphys/kiab156Menon et al. (2019). Arabidopsis FAR-RED ELONGATED HYPOCOTYL 1 and FHY1-LIKE are not required for phytochrome A signal transduction in the nucleus. Plant Communications. Available online 9 November 2019, 100007.Agliassa et al. (2018). Geomagnetic field impacts on cryptochrome and phytochrome signaling. J Photochem Photobiol B. 2018 Aug;185:32-40. doi: 10.1016/j.jphotobiol.2018.05.027.Zhang et al. (2018). Characterization of peanut phytochromes and their possible regulating roles in early peanut pod development. PLoS One. 2018 May 25;13(5):e0198041. doi: 10.1371/journal.pone.0198041.
Special application note:
In vivo pull down assay for PhyA and western blot analysis of eluted proteins is described in Paik et al. (2012). Phytochrome regulates translation of mRNA in the cytosol. PNAS 109 (4): 1335-1340.
Ferredoxins are soluble, iron-sulfur containg proteins that function as electron donors in many metabolic pathways.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Chlamydomonas reinhardtii
Immunogen:
KLH-conjugated synthetic peptide derived from C-terminal part of Chlamydomonas reinhardtii ferredoxin 6
Western blot detection image can be found in reference below
Application Details:
1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 143 l of sterile water
Molecular Weight:
34 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Terauchi et al. (2009). Pattern of expression and substrate specificity of chloroplast ferredoxins from Chlamydomonas reinhardtii. J.Biol. Chem. 38:25867-78.
Special application note:
Western blot detection was done on 20 g of total proteins isolated from chloroplasts
Ferredoxins are soluble, iron-sulfur containg proteins that function as electron donors in many metabolic pathways.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Chlamydomonas reinhardtii
Immunogen:
KLH-conjugated synthetic peptide chosen from a C-terminal of Chlamydomonas reinhardtii Q2HZ24
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Terrauchi et al. (2009). Pattern of expression and substrate specificity of chloroplast ferredoxins from Chlamydomonas reinhardtii. J Biol Chem 284 (38):25867-25878.
RNA polymerase I is a nuclear located DNA-dependent enzyme involved in RNA elongation and regulation of transcription. In yeast subunit A12.2 has been described as homologous to the Rpb9 subunit from polymerase II.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from the Arabidopsis thaliana A12.2 (At3g25940) protein sequence. This sequence is only weakly conserved in other eukaryotic sequences available in the databases.
This antibody is specific for A12,2 subunit of RNA polymerase I but NOT RNA polymerase II or IV from Arabidopsis thaliana
Application Details:
1 : 2000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 143 l of sterile water
Molecular Weight:
13,6 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
This product has previously been labelled as anti-At3g25940 transcription factor S-II (TFIIS) domain-containing protein.Protocol for isolation of cytosolic and nuclear fractions can be found here.
HemH is ptotoporphyrin ferrochelatase of heme biosynthesis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Protein used to elicit this ab shares ca. 50 % homology to Arabidopsis thaliana ferrochelatase 1 and 2.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
50 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Masoumi et al. (2008). Complex formation between protoporphyrinogen IX oxidase and ferrochelatase during haem biosynthesis in Thermosynechococcus elongatus. Microbiol. 154: 3707-3714.
Psb29 (THF1) is a conserved 22 kDa protein which functions in biogenesis of photosystem II complexes. This protein is required for organization of vesicles into mature thylakoid stacks for chloroplast development. Mediates G-protein signalling between the plasma membrane and the plastid.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Nicotiana tabacum, Oryza sativa, Ostreococcus sp., Picea sitcHensis, Populus balsamifera, Physcomitrium patens, Solanum lycopersicum, Solanum tuberosumSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from known sequences of Psb29 including Arabidopsis thaliana Q9SKT0, At2g20890
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Hamel et al. (2016). The chloroplastic protein THF1 interacts with the coiled-coil domain of the disease resistance protein N' and regulates light-dependent cell death. Plant Physiol. 2016 Mar 7. pii: pp.00234.2016Huang et al. (2013). Arabidopsis Thylakoid Formation 1 Is a Critical Regulator for Dynamics ofPSII-LHCII Complexes in Leaf Senescence and Excess Light. Mol Plant. May 13.
Phosphoenolpyruvate carboxykinase (PEPCK, PEP carboxykinase) is an enzyme that catalyses the conversion of oxaloacetate and ATP to phosphoenelpyruvate, carbon dioxide and ADP. PEPCK is encoded by two genes in plants: pck1 and pck2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time. Higher protein load 10-20 g is adviced when working with this antibody.Antibody can be also used following 2D gel electrophoresis.This product can be sold containing ProClin if requested.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
73 | 78 kDa
Not reactive in:
Arabidopsis thaliana, Pinus yunnanensis
Selected references:
Wei et al. (2019). Transcriptomic and proteomic responses to very low CO 2 suggest multiple carbon concentrating mechanisms in Nannochloropsis oceanica. Biotechnol Biofuels 12: 168.Shen et al. (2016). The existence of C4-bundle-sheath-like photosynthesis in the mid-vein of C3 rice. Rice (N Y). 2016 Dec;9(1):20. doi: 10.1186/s12284-016-0094-5. Epub 2016 May 10.Aragón et al. (2013). The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles. Plant Cell Rep. Aug 20. (Ananas comosus, western blot detection following 2D gel electrophoresis)
Glutamine oxoglutarate aminotransferase (abbreviated as GOGAT) is an enzyme involved in synthesis of glutamate from glutamine and alpha-ketoglutarate. GOGAT has two forms in plants: ferredoxin-dependent GOGAT (Fd-GOGAT) and NADH-dependent GOGAT (NADH-GOGAT). 95% of GOGAT found in plants is the Fd-GOGAT type. Fd-GOGAT is encoded by two genes, glu1 and glu2 found on chromosomes 5 and 2 respectively (in Arabidopsis). Fd-GOGAT (both forms) is highly conserved among plants, red algae, and cyanobacteria.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide well conserved in known GOGAT sequences from different species including Arabidopsis thaliana Fd-GOGAT 1 Q9ZNZ7, At5g04140 and Fd-GOGAT 2 Q9T0P4, At2g41220
This antibody can be used as a plastidial marker for leaf material, not roots where levels of GOGAT are too low. A 40 kDa band present in A. thaliana sample is not competed away during antibody neutralization assay. In this assay free peptide used for antibody production is incubated together with anti-GOGAT antibodies. Due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
177 | 170-180 kDa depending upon the species
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Touraine et al. (2019). Iron-sulfur protein NFU2 is required for branched-chain amino acid synthesis in Arabidopsis roots. J Exp Bot. 2019 Mar 27;70(6):1875-1889. doi: 10.1093/jxb/erz050.Wang et al. (2018). Genetic variations in ARE1 mediate grain yield by modulating nitrogen utilization in rice. Nat Commun. 2018 Feb 21;9(1):735. doi: 10.1038/s41467-017-02781-w.Nath et al. (2016). A Nitrogen-Fixing Subunit Essential for Accumulating 4Fe-4S-Containing Photosystem I Core Proteins. Plant Physiol. 2016 Dec;172(4):2459-2470. Epub 2016 Oct 26.Jayawardena et al. (2016). Elevated CO2 plus chronic warming reduces nitrogen uptake and levels or activities of nitrogen -uptake and -assimilatory proteins in tomato roots. Physiol Plant. 2016 Nov 28. doi: 10.1111/ppl.12532. [Epub ahead of print]Takabayashi et al. (2016) Direct interaction with ACR11 is necessary for post-transcriptional control of GLU1-encoded ferredoxin-dependent glutamate synthase in leaves. Sci Rep. 2016 Jul 14;6:29668. doi: 10.1038/srep29668.Yang et al. (2016). Rice Ferredoxin-Dependent Glutamate Synthase Regulates Nitrogen-Carbon Metabolomes and Is Genetically Differentiated between japonica and indica Subspecies. Mol Plant. 2016 Sep 24. pii: S1674-2052(16)30195-2. doi: 10.1016/j.molp.2016.09.004.Moscatelli et al. (2015). Characterisation of detergent-insoluble membranes in pollen tubes of Nicotiana tabacum (L.). Biol Open. 2015 Feb 20. pii: BIO201410249. doi: 10.1242/bio.201410249.Podg rska et al. (2013). Long-term ammonium nutrition of Arabidopsis increases the extrachloroplastic NAD(P)H/NAD(P)+ ratio and mitochondrial reactive oxygen species level in leaves but does not impair photosynthetic capacity. Plant Cell Environ. April 10.
Thioredoxin Reductase (TR, TrxR) is the only known enzyme (EC 1.8.1.9) which is reducing thoredoxin (Trx) Involved in chloroplast protection against oxidative damage. Activity of thoredoxin is essential for growth and survival of the cell. There seem to be one isoform of NtrC protein in Arabidopsis thaliana, which includes an N-terminal reductase domain and a C-terminal domain related to thioredoxin proteins. Arabidopsis thaliana and Chlmydomonas reinhardtii NtrC proteins are ca. 568 amino acids long, but include ca. 80 amino acid signal peptides, for mature protein size of ca. 488 amino acids.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated peptide derived from N-terminal domain of NtrC of Chlamydomonas reinhardtii A8HNQ7The peptide is perfectly conserved in Arabidopsis thaliana NTRC, UniProt: , TAIR: AT2G41680
55.3 kDa (Chlamydomonas reinhardtii), 57.9 kDa (Arabidopsis thaliana), further processed into the matured form
Special application note:
Peptide target chosen from the N-terminal domain, nearly fully conserved within 3-4 NtrA-related proteins from Arabidopsis thalina and with NtrC related proteins from Chlamydomonas reinhardtii and Synechococcus sp. 7942 and other cyanobacteria
Deg1 belongs to S1 chymotrypsin protease family and contains 16 protein members in Arabidopsis thaliana. Deg1 is localized to chloroplast.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Pisum sativum, Zea mays
Expected Species:
Ricinus communis Species of your interest not listed? Contact us
Immunogen:
Recombinant Deg1 of Arabidopsis thaliana O22609, At3g27925, overexpressed in E.coli
Unspecific reaction at ca, 30 kDa and ca, 85 kDa (checked by by mass spec, and confirmed not to be neither Deg1 nor any other protease, The band at the right MW does contain Deg1 by our MS analysis
Application Details:
1 : 500/5 ug thylakoid fraction (WB)
Purity:
Total IgG. Protein G purified in PBS pH 7.4.
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
46,6 | 36 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Zienkiewicz et al. (2013). Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plant Physiol. & Biochem. March 16.
LHL4 is a larger ELIP-like protein with 3 MSRs. Large loop between MSR 2 and 3 makes this protein a distinctive member of the ELIP family. So fat this protein has been only found in green algae.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
One of the several classes of mitochondrial proteases is membrane bound, ATP dependent FtsH protease. Their function is very important for the control of protein quality and quantity by degradation of unassembled subunits. FtsH10 is localized in mitochondria. Alternative names: cell division protease ftsH homolog 10, mitochondrial, AtFtsH10
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide located near C-terminus chosen from sequence of Arabidopsis thaliana FtsH10 Q8VZI8, At1g07510
Applications:
Blue Native PAGE (BN-PAGE), Immunoprecipitation (IP)
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Kolodziejczak et al. (2018). m-AAA Complexes Are Not Crucial for the Survival of Arabidopsis Under Optimal Growth Conditions Despite Their Importance for Mitochondrial Translation. Plant Cell Physiol. 2018 May 1;59(5):1006-1016. doi: 10.1093/pcp/pcy041.Piechota et al. (2015). Unraveling the functions of type II-prohibitins in Arabidopsis mitochondria. Plant Mol Biol. 2015 Apr 21.Kwasniak et al. (2013). Silencing of the Nuclear RPS10 Gene Encoding Mitochondrial Ribosomal Protein Alters Translation in Arabidiopsis Mitochondria. Plant Cell, May 30.Quesada et al. (2011). Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development. Plant J.
Special application note:
Blue-native (2D BN/SDS-PAGE) methodology has been described in Piechota et al, 2010
Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. HSP101 is a cytosolic heat shock protein required for acclimation to high temperature.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Szadeczky-Kardoss et al. (2022) Elongation factor TFIIS is essential for heat stress adaptation in plants. Nucleic Acids Res. 2022 Feb 28;50(4):1927-1950. doi: 10.1093/nar/gkac020. PMID: 35100405; PMCID: PMC8886746.Wang et al. (2022) 17-(Allylamino)-17-demethoxygeldanamycin treatment induces the accumulation of heat shock proteins and alleviates senescence in broccoli. Postharvest Biology and Technology,Volume 186, 2022, 111818, ISSN 0925-5214, https://doi.org/10.1016/j.postharvbio.2021.111818.Fedotova et al. (2020). Influence of high temperatures on heat tolerance and synthesis of heat shock proteins in spring wheat at the initial stages of development // Siberian Journal of Life Sciences and Agriculture. 2020. ?. 12, ? 5. C. 179-191. DOI: 10.12731/2658-6649-2020-12-5-179-191Gorovits et al. (2020). Pharmaceuticals in treated wastewater induce a stress response in tomato plants. Sci Rep. 2020 Feb 5;10(1):1856. doi: 10.1038/s41598-020-58776-z.McLoughlin et al. (2019) HSP101 Interacts with the Proteasome and Promotes the Clearance of Ubiquitylated Protein Aggregates. Plant Physiol. 2019 Aug;180(4):1829-1847. doi: 10.1104/pp.19.00263
Hsp17.6 belongs to a family of class I of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
There are six total class I genes, Essentially this antibody might react to some extent with all of them, But does not react with class II, organelle, or any other shsp classes
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
17.6 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Swetha et al. (2021) Single and Combined Salinity and Heat Stresses Impact Yield and Dead Pericarp Priming Activity. Plants (Basel). 2021 Aug 8;10(8):1627. doi: 10.3390/plants10081627. PMID: 34451672; PMCID: PMC8399105.Siddiqui et al. (2020). Melatonin and calcium function synergistically to promote the resilience through ROS metabolism under arsenic-induced stress. Journal of Hazardous MaterialsVolume 398, 5 November 2020, 122882McLoughlin et al. (2019) HSP101 Interacts with the Proteasome and Promotes the Clearance of Ubiquitylated Protein Aggregates. Plant Physiol. 2019 Aug;180(4):1829-1847. doi: 10.1104/pp.19.00263Kato et al. (2019). Induction of the heat shock response in Arabidopsis by chlorinated 1,4-naphthoquinones. Plant Growth Regul (2019). https://doi.org/10.1007/s10725-019-00477-3. Alamri et al. (2018). Nitric oxide-mediated cross-talk of proline and heat shock proteins induce thermotolerance in Vicia faba L. Environmental and Experimental Botany Available online 23 June 2018.
Special application note:
This product can be sold containing ProClin if requested
Hsp17.7 belongs to a family of class II of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Dicots, Fraxinus sp.Species of your interest not listed? Contact us
Immunogen:
full length recombinant protein produced in E. coli and purified by conventional methods (no affinity tag). Arabidopsis thaliana Hsp17.7 CII (class two), UniProt: O81822, TAIR: At5g12030
Cha et al. (2020). Humic acid enhances heat stress tolerance via transcriptional activation of Heat-Shock Proteins in Arabidopsis. Sci Rep. 2020 Sep 14;10(1):15042.doi: 10.1038/s41598-020-71701-8. McLoughlin et al. (2019) HSP101 Interacts with the Proteasome and Promotes the Clearance of Ubiquitylated Protein Aggregates. Plant Physiol. 2019 Aug;180(4):1829-1847. doi: 10.1104/pp.19.00263Fu et al. (2019). Increased fes1a thermotolerance is induced by BAG6 knockout. Plant Mol Biol. 2019 Feb 22. doi: 10.1007/s11103-019-00844-8.Korotaeva et al. (2018). Effect of Heat Hardening on Expression of Genes phb3 and phb4 and Accumulation of Phb Proteins in Green Leaves of Arabidopsis thaliana. Russian Journal of Plant Physiology, 65(5), 688-696, 2018. https://doi.org/10.1134/s1021443718040039McLoughlin et al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.
The plant SAL1 (known also as FIERY1, FRY1, HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 2, HOS2, MBM17.8, MBM17_8) is a 353 aa protein homologous to the HAL2 and CysQ phosphatases of yeast and Escherichia coli, respectively. The SAL1 protein expressed in E. coli shows nucleotidase and inositol phosphatase activities. SAL1 is proposed to participate in the sulfur assimilation pathway as well as in the phosphoinositide signaling pathway.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Glycine max , Lycopersicum esculentum, Nicotiana tabaccum, Populus tremula
Expected Species:
Gossypium hirsutum, Oryza sativa Species of your interest not listed? Contact us
Immunogen:
Recombinant SAL1, full-length protein, 353 amino acids. The cDNA of SAL1 (At5g63980, protein Q42546) was cloned into pHUE expression vector and the protein has been produced and purified according to Baker et al 2005
Chan et al. (2016). Sensing and signaling of oxidative stress in chloroplasts by inactivation of the SAL1 phosphoadenosine phosphatase. Proc Natl Acad Sci U S A. 2016 Aug 2;113(31):E4567-76. doi: 10.1073/pnas.1604936113. Epub 2016 Jul 18.
PRK ribulose-5-P-kinase| phosphoribulokinase is an enzyme that catalyzes the chemical reaction ATP + D-ribulose 5-phosphate to ADP + D-ribulose 1,5-bisphosphate.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Antibody detects PRK using a load from 4-20 g/well of a chloroplast fraction, incubation over night at 4 C
Application Details:
1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
44 | 39 kDa (A. thaliana)
Not reactive in:
Proteobacteria
Selected references:
Fukayama et al. (2018). Expression level of Rubisco activase negatively correlates with Rubisco content in transgenic rice. Photosynth Res. 2018 May 30. doi: 10.1007/s11120-018-0525-9.P rez-Ruiz et al. (2017). NTRC-dependent redox balance of 2-Cys peroxiredoxins is needed for optimal function of the photosynthetic apparatus. Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):12069-12074. doi: 10.1073/pnas.1706003114.Rai et al. (2017). Real-time iTRAQ-based proteome profiling revealed the central metabolism involved in nitrogen starvation induced lipid accumulation in microalgae. Sci Rep. 2017 Apr 5;7:45732. doi: 10.1038/srep45732. (microalga, western blot)Nikkanen et al. (2016). Crosstalk between chloroplast thioredoxin systems in regulation of photosynthesis. Plant Cell Environ. 2016 Aug;39(8):1691-705. doi: 10.1111/pce.12718.
Special application note:
Antibody can be used as a marker of chloroplast stroma
PRK ribulose-5-P-kinase| phosphoribulokinase is an enzyme that catalyzes the chemical reaction ATP + D-ribulose 5-phosphate to ADP + D-ribulose 1,5-bisphosphate.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
PRK ribulose-5-P-kinase| phosphoribulokinase is an enzyme that catalyzes the chemical reaction ATP + D-ribulose 5-phosphate to ADP + D-ribulose 1,5-bisphosphate.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
PRK ribulose-5-P-kinase| phosphoribulokinase is an enzyme that catalyzes the chemical reaction ATP + D-ribulose 5-phosphate to ADP + D-ribulose 1,5-bisphosphate.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight 488.
Molecular Weight:
44 | 39 kDa (A. thaliana)
Not reactive in:
Proteobacteria
Selected references:
To be added when available. Antibody released in May 2023.
Special application note:
Antibody can be used as a marker of chloroplast stroma.DyLight 488 Amax = 493 nm, Emax = 519 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
PRK ribulose-5-P-kinase| phosphoribulokinase is an enzyme that catalyzes the chemical reaction ATP + D-ribulose 5-phosphate to ADP + D-ribulose 1,5-bisphosphate.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight 594.
Molecular Weight:
44 | 39 kDa (A. thaliana)
Not reactive in:
Proteobacteria
Selected references:
To be added when available. Antibody released in May 2023.
Special application note:
Antibody can be used as a marker of chloroplast stroma.DyLight 594 has Amax = 593 nm, Emax = 618 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
PRK ribulose-5-P-kinase phosphoribulokinase is an enzyme that catalyzes the chemical reaction ATP + D-ribulose 5-phosphate to ADP + D-ribulose 1,5-bisphosphate.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight 650.
Molecular Weight:
44 | 39 kDa (A. thaliana)
Not reactive in:
Proteobacteria
Selected references:
To be added when available. Antibody released in May 2023.
Special application note:
Antibody can be used as a marker of chloroplast stroma.DyLight 650 has Amax = 652 nm, Emax = 672 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
PRK ribulose-5-P-kinase| phosphoribulokinase is an enzyme that catalyzes the chemical reaction ATP + D-ribulose 5-phosphate to ADP + D-ribulose 1,5-bisphosphate
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Lipoxygenases (LOXs; EC 1.13.11.12, synonym: lipoxydases) are a family of enzymes that catalyze oxygenation of polyunsaturated fatty acids (PUFAs) into lipidhydroperoxides (LOOHs) involved in responses to stresses. LOXs has been found to play a role in plant growth and development, senescence as well as can be activated in response to environamental stress (drought, heavy metals). Alternative name of the antigen: Lipoxygenase 2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Vitis vinifera
Expected Species:
Brassica napus, Musa acuminata subsp. malaccensis Species of your interest not listed? Contact us
Aweak band at around 84 kDa is detected as a probable result of cross-reaction with another lipoxygenase
Application Details:
1 : 50 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
102 | 97 kDa
Not reactive in:
Chlamydomonas reinhardtii
Selected references:
Seguel et al. (2018). PROHIBITIN 3 forms complexes with ISOCHORISMATE SYNTHASE 1 to regulate stress-induced salicylic acid biosynthesis in Arabidopsis. Plant Physiol. Jan 2018. DOI:10.1104/pp.17.00941Cecchini et al. (2018). Underground azelaic acid-conferred resistance to Pseudomonas syringae in Arabidopsis. Mol Plant Microbe Interact. 2018 Aug 29. doi: 10.1094/MPMI-07-18-0185-R. (antibody used on LOX2 mutant plant)Pilati et al. (2015). The onset of grapevine berry ripening is characterized by ROS accumulation and lipoxygenase-mediated membrane peroxidation in the skin. BMC Plant Biol. 2014 Apr 2;14:87. doi: 10.1186/1471-2229-14-87.
Rubisco catalyzes the rate-limiting step of CO2 fixation in photosynthesis. This enzyme contains two subunits, each present in eight copies. In plants and green algae, 55-kD large subunit is coded by the chloroplast rbcL gene, and the 15-kD small subunit is coded by a family of nuclear RbcS genes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Algae, Camellia oleifera, Erythranthe guttata, Flaveria bidentis, Flaveria sonorensis, Glycine max, L, Marchantia paleacea, Musa acuminata, Nicotiana benthamiana, Oryza sativa, Petunia hybrida, Polianthes tuberosa, Populus deltoides, Triticum aestivum, Solanum melongena, Solanum tuberosum, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from all known sequences of RbcS from monocots and dicots including RuBisCO small subunit 1A UniProt: P10795,TAIR: AT1G67090, and 1B of Arabidopsis thaliana UniProt: P10796At5g38430
This product can be sold containing ProClin if requested
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
20 | 15 kDa
Not reactive in:
Cyanobacteria
Selected references:
Lim et al (2022). Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13(1):652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Mazur et al. (2021) The SnRK2.10 kinase mitigates the adverse effects of salinity by protecting photosynthetic machinery. Plant Physiol. 2021 Dec 4;187(4):2785-2802. doi: 10.1093/plphys/kiab438. PMID: 34632500; PMCID: PMC8644180.Bernau et al. (2021) Precision analysis for the determination of steric mass action parameters using eight tobacco host cell proteins,Journal of Chromatography A,Volume 1652,2021,462379,ISSN 0021-9673,https://doi.org/10.1016/j.chroma.2021.462379. (https://www.sciencedirect.com/science/article/pii/S0021967321005033)Ma et al. (2020). An ortholog of the Vasa intronic gene is required for small RNA-mediated translation repression in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2020 Jan 7;117(1):761-770. doi: 10.1073/pnas.1908356117.Akmouche et al. (2019). Do nitrogen- and sulphur-remobilization-related parameters measured at the onset of the reproductive stage provide early indicators to adjust N and S fertilization in oilseed rape (Brassica napus L.) grown under N- and/or S-limiting supplies? Planta. 2019 Dec;250(6):2047-2062. doi: 10.1007/s00425-019-03284-2.
The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Do not Store this antibody in 4°C.
VERY IMPORTANT: Please, do not heat up your samples above 70 C as this may cause H+ATPase to precipitate, and there will be no signal on your Western Blot. Before SDS-PAGE, centrifuge your samples at room temperature at 10 000 rpm/1 min to remove any aggregates. H+ATPase will be less abundant in mature roots and leafs and therefore detection may require use of very sensitive reagents. This product can be sold containing ProClin if requested.
Michalopoulou et al. (2022) The host exocyst complex is targeted by a conserved bacterial type-III effector that promotes virulence. Plant Cell. 2022 May 30:koac162. doi: 10.1093/plcell/koac162. Epub ahead of print. PMID: 35640532.Hofmann, Wienkoop & Luthje (2022) Hypoxia-Induced Aquaporins and Regulation of Redox Homeostasis by a Trans-Plasma Membrane Electron Transport System in Maize Roots. Antioxidants (Basel). 2022 Apr 25;11(5):836. doi: 10.3390/antiox11050836. PMID: 35624700; PMCID: PMC9137787.Huang et al (2021). Parasitic modulation of host development by ubiquitin-independent protein degradation. Cell. 2021 Sep 30;184(20):5201-5214.e12. doi: 10.1016/j.cell.2021.08.029. Epub 2021 Sep 17. PMID: 34536345; PMCID: PMC8525514.Lapshin et al. (2021) Sterol Extraction from Isolated Plant Plasma Membrane Vesicles Affects H+-ATPase Activity and H+-Transport. Biomolecules. 2021 Dec 16;11(12):1891. doi: 10.3390/biom11121891. PMID: 34944535; PMCID: PMC8699270.Choi et al. (2021) Augmented CO2 tolerance by expressing a single H+-pump enables microalgal valorization of industrial flue gas. Nat Commun. 2021 Oct 18;12(1):6049. doi: 10.1038/s41467-021-26325-5. PMID: 34663809; PMCID: PMC8523702.
The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.This antibody is directly conjugated to ALP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
VERY IMPORTANT: Please, do not heat up your samples above 70 C as this may cause H+ATPase to precipitate, and there will be no signal on your Western Blot. Before SDS-PAGE, centrifuge your samples at room temperature at 10 000 rpm/1 min to remove any aggregates. H+ATPase will be less abundant in mature roots and leafs, and therefore detection may require use of very sensitive reagents.
Application Details:
1 : 100 (IL), 1 : 1000-1 : 5000 (WB)
Purity:
Antigen affinity purified serum in PBS pH 7.4, conjugated to ALP.
Molecular Weight:
90- 95 kDa (Arabidopsis thaliana, depending upon an isoform)
The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.This antibody is directly conjugated to Biotin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
VERY IMPORTANT: Please, do not heat up your samples above 70 C as this may cause H+ATPase to precipitate, and there will be no signal on your Western Blot. Before SDS-PAGE, centrifuge your samples at room temperature at 10 000 rpm/1 min to remove any aggregates. H+ATPase will be less abundant in mature roots and leafs, and therefore detection may require use of very sensitive reagents.
Application Details:
1 : 100 (IL), 1 : 1000-1 : 5 000 (WB)
Purity:
Antigen affinity purified serum in PBS pH 7.4, conjugated to Biotin.
Molecular Weight:
90- 95 kDa (Arabidopsis thaliana, depending upon an isoform)
The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
VERY IMPORTANT: Please, do not heat up your samples above 70 C as this may cause H+ATPase to precipitate, and there will be no signal on your Western Blot. Before SDS-PAGE, centrifuge your samples at room temperature at 10 000 rpm/1 min to remove any aggregates. H+ATPase will be less abundant in mature roots and leafs, and therefore detection may require use of very sensitive reagents.
Application Details:
1 : 600-1 : 1000 (IF), 1 : 1000-1 : 2 000 (WB)
Purity:
Antigen affinity purified serum in PBS pH 7.4, conjugated to DyLight 488.
Molecular Weight:
90- 95 kDa (Arabidopsis thaliana, depending upon an isoform)
To be added when available. Antibody released in October 2023.
Special application note:
Cellular [compartment marker] for plasma membrane.DyLight 488 has Amax = 493 nm, Emax = 518 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
VERY IMPORTANT: Please, do not heat up your samples above 70 C as this may cause H+ATPase to precipitate, and there will be no signal on your Western Blot. Before SDS-PAGE, centrifuge your samples at room temperature at 10 000 rpm/1 min to remove any aggregates. H+ATPase will be less abundant in mature roots and leafs, and therefore detection may require use of very sensitive reagents.
Application Details:
1 : 600-1 : 1000 (IF), 1 : 1000-1 : 5 000 (WB)
Purity:
Antigen affinity purified serum in PBS, pH 7.4, conjugated to DyLight 594.
Molecular Weight:
90- 95 kDa (Arabidopsis thaliana, depending upon an isoform)
To be added when available. Antibody released in October 2023.
Special application note:
Cellular [compartment marker] for plasma membrane.DyLight 594 has Amax = 593 nm, Emax = 618 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
VERY IMPORTANT: Please, do not heat up your samples above 70 C as this may cause H+ATPase to precipitate, and there will be no signal on your Western Blot. Before SDS-PAGE, centrifuge your samples at room temperature at 10 000 rpm/1 min to remove any aggregates. H+ATPase will be less abundant in mature roots and leafs, and therefore detection may require use of very sensitive reagents.
Application Details:
1 : 600-1 : 1000 (IF), 1 : 1000-1 : 5 000 (WB)
Purity:
Antigen affinity purified serum in PBS pH 7.4, conjugated to DyLight 650.
Molecular Weight:
90- 95 kDa (Arabidopsis thaliana, depending upon an isoform)
To be added when available. Antibody released in October 2023.
Special application note:
Cellular [compartment marker] for plasma membrane.DyLight 650 has Amax = 652 nm, Emax = 672 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.This antibody is directly conjugated to HRP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
VERY IMPORTANT: Please, do not heat up your samples above 70 C as this may cause H+ATPase to precipitate, and there will be no signal on your Western Blot. Before SDS-PAGE, centrifuge your samples at room temperature at 10 000 rpm/1 min to remove any aggregates. H+ATPase will be less abundant in mature roots and leafs, and therefore detection may require use of very sensitive reagents.
Application Details:
1 : 600-1 : 1000 (IF), 1 : 1000-1 : 5 000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP.
Molecular Weight:
90- 95 kDa (Arabidopsis thaliana, depending upon an isoform)
eEF1B-beta protein belongs to a family of elongation factors, proteins which are involved in translational elongation. eEF1B-beta protein is localized in plant cytosol. Alternative names: EF-1-delta 2, elongation factor 1B-beta 2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
Brassica campestris, Hordeum vulgare, Nicotiana attenuata, Oryza sativa Pisum sativum, Ricinus communisSpecies of your interest not listed? Contact us
Immunogen:
Recombinant eEF1B-beta protein from Arabidopsis thaliana with no affinity tag, UniProt: Q9SI20,TAIR: At2g18110
No confirmed exceptions from predicted reactivity are currently known
Selected references:
L zaro-Mixteco et al. (2012). The Absence of Heat Shock Protein HSP101 Affects the Proteome of Mature and Germinating Maize Embryos. J. Proteome Res. May 11, ahead of print.
SMT1 (EC=2.1.1.41) is an enzyme involved in steroid biosynthesis localized specific to the Endoplasmic Reticulum (ER), Boutte et al., 2009 . It is catalyzing the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol. The protein is highly expressed in vascular tissue, mature leaves and in regions undergoing cellular expansion. Alternative names: Cycloartenol-C-24-methyltransferase, 24-sterol C-methyltransferase 1, Protein STEROL METHYLTRANSFERASE 1, Protein CEPHALOPOD
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Amborella trichopoda, Brassica napus, Brassica rapa, Capsella rubella, Citrus clementina, Eutrema salsugineum, Glycine max, Glycine soja, Gossypium mexicanum, Medicago truncatula, Populus trichopocarpa, Prunus persica, Ricinus communis, Theobroma cacao, Vitis vinifera Species of your interest not listed? Contact us
SMT1 antibody characterization in Western Blot and immunofluorescence labeling: Boutt Y et al. (2010). Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis. EMBO J. 29, 5465-5458.
Ming-fang et al. (2021) Improved quantification of immune-gold labeling and its use to compare the distribution of cellular factors among sub-chloroplast compartments,Micron,2021,103060,ISSN 0968-4328,https://doi.org/10.1016/j.micron.2021.103060.Cano-Ramirez et al. (2021) M. Plasma Membrane Fluidity: An Environment Thermal Detector in Plants. Cells. 2021 Oct 17;10(10):2778. doi: 10.3390/cells10102778. PMID: 34685758; PMCID: PMC8535034.Collins et al. (2020). EPSIN1 Modulates the Plasma Membrane Abundance of FLAGELLIN SENSING2 for Effective Immune Responses . Plant Physiol. 2020 Feb 24. pii: pp.01172.2019. doi: 10.1104/pp.19.01172Laohavisit (2020). Quinone perception in plants via leucine-rich-repeat receptor-like kinases. Nature. 2020 Nov;587(7832):92-97. doi: 10.1038/s41586-020-2655-4. Epub 2020 Sep 2. PMID: 32879491.Yang et al. (2016). Arabidopsis PROTEASOME REGULATOR1 is required for auxin-mediated suppression of proteasome activity and regulates auxin signalling. Nat Commun. 2016 Apr 25;7:11388. doi: 10.1038/ncomms11388.
Special application note:
SMT1 is an integral membrane protein of ER (Boutte et al., 2009) while BiP is a membrane associated protein.Endogenous SMT1 is expressed at very low levels in root epidermis and root cap as compare to cortex and endodermis and therefore this can contribute to SMT1 detection problems.
This antibody specifically cross-reacts against xylose residues bound to the protein N-glycans in beta1,2. This residue is characterisitc of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Negative control: Fetuin, a glycoprotein containing fucose linked in alpha 1.6 and no xylose, Sigma, product number F3385.Positive control: Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250
Application Details:
1 g/ml (ELISA), 2 g/10 ml incubation buffer (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
10-100 for various glycoproteins
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Xavier et al. (2021) Inactivation of N-Acetylglucosaminyltransferase I and a1,3-Fucosyltransferase Genes in Nicotiana tabacum BY-2 Cells Results in Glycoproteins With Highly Homogeneous, High-Mannose N-Glycans. Frontiers in Plant Science. Volume 12. doi: 10.3389/fpls.2021.634023Yang et al. (2021). Golgi-localized manganese transporter PML3 regulates Arabidopsis growth through modulating Golgi glycosylation and cell wall biosynthesis. New Phytol. 2021 Jan 17. doi: 10.1111/nph.17209. Epub ahead of print. PMID: 33454966.Lucas et al. (2019). Multiple xylosyltransferases heterogeneously xylosylate protein N-linked glycans in Chlamydomonas reinhardtii. Plant J. 2019 Nov 27. doi: 10.1111/tpj.14620. Lucas et al. (2019). Multiple xylosyltransferases heterogeneously xylosylate protein N-linked glycans in Chlamydomonas reinhardtii. Plant J. 2019 Nov 27. doi: 10.1111/tpj.14620.Hou et al. (2019). Identification and characterization of a novel glycoprotein core xylosidase from the bacterium Elizabethkingia meningoseptica. Biochemical and Biophysical Research Communications Available online 27 July 2019.
Special application note:
Beta (1,2) xylose is present exclusively in plant N-glycans so antibodies against this sugar moiety should not cross-react with any mammal glycoprotein.This antibody do not bind free D-xylose. This antibody does not seem to work in immunolocalization.
This antibody specifically cross-reacts against fucose residues bound to the protein N-glycans in alpha 1,3. This residue is characteristic of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Higher plants, Pheodactylum tricornutum (diatom)
Expected Species:
Higher plants, Phaeodactylum tricornutum
Immunogen:
Core fucose residues bound to the N-glycan in alpha 1,3
Applications:
ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
Controls:PLA2 (phospholipase 2 from bee venom) which contains only α1.3 fucose, Sigma, product number P9279.Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250.The antibody does not recognize alpha 1,6-fucose.
Application Details:
0,5 g/ml (ELISA), 1 : 40 (IL), 1 ug/10 ml (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
10 - 100 for various glycoproteins
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Zhang et al. (2018). An important role of L -fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity. New Phytol. 2018 Dec 15. doi: 10.1111/nph.15639.Jansing et al. (2018). CRISPR/Cas9-mediated knockout of six glycosyltransferase genes in Nicotiana benthamiana for the production of recombinant proteins lacking β-1,2-xylose and core α-1,3-fucose. Plant Biotechnol J. 2018 Jul 3. doi: 10.1111/pbi.12981.Nakanishi et al. (2017). Protection of Human Colon Cells from Shiga Toxin by Plant-based Recombinant Secretory IgA. Sci Rep. 2017 Apr 3;7:45843. doi: 10.1038/srep45843. (ELISA)Hanania et al. (2017). Establishment of a tobacco BY2 cell line devoid of plant specific xylose and fucose as a platform for the production of biotherapeutic proteins. Plant Biotechnol J. 2017 Feb 3. doi: 10.1111/pbi.12702.Ebert et al. (2015). Identification and Characterization of a Golgi-Localized UDP-Xylose Transporter Family from Arabidopsis. Plant Cell. 2015 Mar 24. pii: tpc.114.133827.Lehtim ki et al. (2014). Posttranslational modifications of FERREDOXIN-NADP+ OXIDOREDUCTASE in Arabidopsis chloroplasts. Plant Physiol. 2014 Dec;166(4):1764-76. doi: 10.1104/pp.114.249094Baiet et all. (2010). N-glycans of Phaeodactylum tricornutum diatom and functional characterization of its N-acetylglucosaminyltransferase I enzyme. J Biol. Chem. Dec 17.
Special application note:
Alpha (1,3) fucose is present not only in plants but also in some invertebrates (such as nematodes, bees, etc,) , However, cross-reaction with glycoproteins from these organisms is weaker than the one observed in plants, This sugar residue does not exist in mammals, in their endogenous glycoproteins
Procaryotic Hsp70 protein family includes DnaK, HscA (Hsc66), HscC (Hsc62). Those proteins are involved in protein folding, cell protection from environmental stress and other functions which are vital for a living cell.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
It is not determined which isoform of DnaK is recognized by this antibody in Arabidopsis thaliana.
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
70 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
G hre et al. (2006). One of Two Alb3 Proteins Is Essential for the Assembly of the Photosystems and for Cell Survival in Chlamydomonas The Plant Cell 18:1454–1466.
DNAJ (Dna J) is prokaryotic heat shock protein which interacts with Hsp70-like protein DnaK in E. coli. This protein is involved in a variety of different biological processes in a living cell including protein transport cell response to high temperatures. Alternative name: chloroplast DnaJ-like protein 1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Chlamydomonas reinhardtii
Immunogen:
recombiant DNAJ of Chlamydomonas reinhardtii Q66YD3
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Siddiqui et al. (2020). Melatonin and calcium function synergistically to promote the resilience through ROS metabolism under arsenic-induced stress. Journal of Hazardous MaterialsVolume 398, 5 November 2020, 122882G hre et al. (2006). One of Two Alb3 Proteins Is Essential for the Assembly of the Photosystems and for Cell Survival in Chlamydomonas The Plant Cell 18:1454–1466.
Ycf3 together with Ycf4 have been found as extrinistic thylakoid membrane proteins which are required for the accumulation of PSI complex in Chlamydomonas reinhardii.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii, cyanobacteria
Expected Species:
Algae, Arabidopsis thaliana, Avena sativa, Chlorella vulgaris, Marchantia polymorpha, Phaseolus vulgaris, Physcomitrium patens, Chlorokybus atmophyticus, Ostreococcus tauriSpecies of your interest not listed? Contact us
Immunogen:
full length recombinant ycf3 protein of Chlamydomonas reinhardtii UniProt: O20031, as described in Boudreau et al. 1997
Western blot detection image can be found in Boudreau et al. 1997.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
19 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113. Epub 2016 Feb 22.Naver et al. (2001). Functional studies of Ycf3. The Plant Cell 13:2731- 2746.Boudreau et al. (1997) The chloroplast ycf3 and ycf4 open reading frames of Chlamydomonas reinhardtii are required for the accumulation of the photosystem I complex. The EMBO J.16:6095-6104.
Ycf3 together with Ycf4 have been found as extrinistic thylakoid membrane proteins which are required for the accumulation of PSI complex in Chlamydomonas reinhardii.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii, cyanobacteria
Expected Species:
Chlamydomonas reinhardtii, CyanobacteriaSpecies of your interest not listed? Contact us
Immunogen:
full length recombinant ycf4 protein of Chlamydomonas reinhardtii UniProt: O20030 as described in Boudreau et al. 1997
Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113Naver et al. (2001). Functional studies of Ycf3. The Plant Cell 13:2731- 2746.Boudreau et al. (1997) The chloroplast ycf3 and ycf4 open reading frames of Chlamydomonas reinhardtii are required for the accumulation of the photosystem I complex. The EMBO J.16:6095-6104.
Phosphate acetyltransferase (PTA) - EC=2.3.1.8 is an enzyme from transferase family which participates in three metabolic pathways: taurine and hypotaurine metabolism, pyruvate metabolism and propanoate metabolism. Alternative names: acetyl-CoA:phosphate acetyltransferase, phosphotransacetylase, phosphoacylase.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Volvox carteri Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide conjugated derived from PTA2 of Chlamydomonas reinhardtii A8IZZ9
Allophycocyanin is a protein of the light-harvesting phycobiliprotein family of red algae and cyanobacteria. It is an accessory pigment to chlorophyll A located in the core of the phycobilisome, and its strong spectral overlap with chlorophyll facilitates energy transfer for photosynthesis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Porphyridium cruentum, Synechocystis PCC 6803
Expected Species:
Red Algae, CyanobacteriaSpecies of your interest not listed? Contact us
Immunogen:
native allophycocyanin alpha and beta purified from Porphyridium cruentum phycobilisomes
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Ge at al. (2017). Translating Divergent Environmental Stresses into a Common Proteome Response through the Histidine Kinase 33 (Hik33) in a Model Cyanobacterium. Mol Cell Proteomics. 2017 Jul;16(7):1258-1274. doi: 10.1074/mcp.M116.068080.Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526.Gunnelius et al. (2014). The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. Nucleic Acids Res. 2014 Jan 29.Hernandez-Prieto et al. (2011). The small CAB-like proteins of the cyanobacterium Synechocystis sp. PCC 6803: Their involvement in chlorophyll biogenesis for Photosystem II. Bioch.Bioph. Acta.Gantt & Lipschultz (1974). Phycobilisome structure by immuno-electron microscopy. J. Phycology, Vol. 13:3, pages: 185-192. (immunolocalization)Gantt & Lipschultz (1974). Phycobilisomes of Porphyridium cruentum: Pigment Analysis. Biochem. 13:2960. Gantt E & C Lipschultz (1977). Probing phycobilisome structure by immuno-electron microscopy. J Phycol. 13:18
Special application note:
This product can be sold containing proclin if requested
Phycocyanin is a phycobiliprotein in the light-harvesting complexes (phycobilisomes) of red algae and cyanobacteria. Its alpha and beta subunits contain phycocyanobilin chromophores, and in red algal R-PC they also contain phycoerythrobilin chromophores.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Porphyridium cruentum
Expected Species:
Algae (red), Cyanobacteria Species of your interest not listed? Contact us
Immunogen:
native R-PC purified from phycobilisomes of Porphyridium cruentum
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Gunnelius et al. (2014). The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. Nucleic Acids Res. 2014 Jan 29.Gantt & Lipschultz (1974). Phycobilisomes of Porphyridium cruentum: Pigment Analysis. Biochem. 13:2960. Gantt E and C Lipschultz (1977). Probing phycobilisome structure by immuno-electron microscopy. J Phycol. 13:18
In phycobilisomes, the light-harvesting antennae of red algae, b-phycoerythrin consists of alpha and beta chains that contain phycoerythrobilin chromophores (an open-chain tetrapyrolle, red colored). It is found in chloroplasts of many red algae, and is closely related to the PEs of cyanobacteria and cryptomonads.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Porphyridium cruentum
Expected Species:
Algae (red), Cyanobacteria, Cryptomonads Species of your interest not listed? Contact us
Immunogen:
native purified b-phycoerythrin of Porphyridium cruentum (protein with attached phycobilisomes)
B-PE is a phycobiliprotein which is a building block of phycobilisomes, the light-harvesting complex, in red algae and some cyanobacteria. Phycobilisomes are attached to the stromal side of the thylakoid membrane. B-phycoerythrin has both phycoerythrobilin (on alpha and beta subunits) and phycourobilin (on the gamma subunits) chromophores.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Porphyridium cruentum
Expected Species:
Algae (red), Cyanobacteria, Cryptomonads Species of your interest not listed? Contact us
Immunogen:
native purified B-phycoerythrin of Porphyridium cruentum with attached chromophores
A large polypeptide Lcm with a few phycocyanobilin chromophores which acts as a terminal energy acceptor and as a linker polypeptide between the phycobilisomes and the photosynthetic reaction centres (PS2).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Redlinger & Gantt (1982) A Mr 95,000 polypeptide in Porphyridium cruentum phycobilisomes and thylakoids: PNAS 79:5542.Redlinger & Gantt (1981). Phycobilisome structure of Porphyridium cruentum. Plant Physiol. 68:1375.
Special application note:
Anabaena sp. sample can serve as a negative control, as under nitrogen deficient conditions phycobiliproteins are going to be lost. Overall sample quality is of crucial importance and in older or not properly stored samples, phycobiliproteins will undergo proteolytic degradation.
The light-harvesting system is composed of pigment-binding proteins belonging to the Lhc multigenic family. Lhc polypeptides are able to bind chlorophyll a, chlorophyll b, and xanthophyll molecules in a suitable structural and dynamic mutual arrangement, ensuring high efficiency of energy transfer processes involved in light-harvesting and photoprotection.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Strongly reactive to 7 Lhc1 light harvesting polypeptides of P. cruentum
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
20-25 kDa
Not reactive in:
Higher plants
Selected references:
Tan et al. (1995).Decrease of polypeptides in the PS I antenna complex with increasing growth irradiance in the red alga Porphyridium cruentum. Photosyn. Research 45:1.Wolfe et al. (1994) Evidence for a common origin of chloroplasts with light-harvesting complexes of different pigmentation. Nature 367:566
Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. In spite of expression during heat stress members of HSP100 protein family are also expressed during seed development.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4 C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Glycne max, Nicotiana tabacum, Vitis vinifera Species of your interest not listed? Contact us
Hsp17.6 belongs to a family of class I of a small heat shock proteins. They are induced once a plant cells are stressed by an increased temperature. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small hsp function also in a late seed maturation process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4 C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Arabidopsis thaliana
Immunogen:
Recombinant protein derived from a sequence Arabidopsis thaliana HSP17.6 Ci (class one) UniProt: P13853, TAIR: At1g53540
There are six total class I genes, Essentially this antibody might react to some extent with all of them, But does not react with class II, organelle, or any other shsp classes
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Mao and Sun (2015). Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3. J Exp Bot. 2015 May 26. pii: erv244.
HSP21 is a chloroplast-localized small heat shock protein. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Please, note that there might be no HSPs accumulation below temperature of 32-34 C, HSPs are induced when the plant experience temperatures higher than the growing temperature with around 10 C, So, the HSPs induction temperatures for plants grown at 18C differ from these for plants grown at 24C,Another very effective parameter is the humidity, When using low humidity the plant has a chance to cool down through transpiration, In this case the HSPs induction requires higher temperatures
Application Details:
1 : 3000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
25 | 21 kDa
Not reactive in:
Chlamydomonas reinhardtii
Selected references:
Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)L mke et al. (2016). A hit-and-run heat shock factor governs sustained histone methylation and transcriptional stress memory. EMBO J. 2016 Jan 18;35(2):162-75. doi: 10.15252/embj.201592593. Epub 2015 Dec 9.McLoughlin et al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.Shen et al. (2016). The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability. Plant J. 2016 Aug 19. doi: 10.1111/tpj.13310. [Epub ahead of print]Almoguera et al. (2015). Heat shock transcription factors involved in seed desiccation tolerance and longevity retard vegetative senescence in transgenic tobacco. Planta. 2015 May 29.Hai-Dong Yu et al. (2012). Downregulation of Chloroplast RPS1 Negatively Modulates Nuclear Heat-Responsive Expression of HsfA2 and Its Target Genes in Arabidopsis. Plos Genetics.
Special application note:
Recommended load per well 20 g of total proteinThis product can be sold containing proClin if requested.
Hsp16.6 belongs to a family of class I of a small heat shock proteins. They are induced once a cell is stressed by an increased temperature. Synechocystis Hsp16.6 is involved in the development of thermotolerance and thylakoid stability. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Synechocystis sp. PCC 6803
Expected Species:
Synechococcus sp., Thermosynechococcus sp.Species of your interest not listed? Contact us
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Gunnelius et al. (2014). The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. Nucleic Acids Res. 2014 Jan 29.
Special application note:
This product can be sold containing proclin if requested
Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. In spite of expression during heat stress members of HSP100 protein family are also expressed during seed development.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Agave tequilana, Citrus sp., Cucumis sativus L. var Krak
When using this antibody on a new species with this antibody perform a following experiment: prepare a leaf extract from a leaf without stress and one that has been heat stressed as follows: Take a whole leaf (in the case of plants with small leaves, like Arabidopsis thaliana), or part of a leaf and place on wet filter paper in a petri dish. Heat stress in the dark at 38 C/90 minutes to 2 hours and then allow to recover for 2 hours at room temperature in low light (leave it on the lab bench). You should be able to load 10 ug for western blotting and compare the non-heat stressed to the heat stressed sample. Use Arabidopsis thaliana leaf as a positive control. Following such treatment 1 ug of a total protein from Arabidopsis thaliana allows detection of HSP101.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
101 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Bychkov et al. (2022) The role of PAP4/FSD3 and PAP9/FSD2 in heat stress responses of chloroplast genes. Plant Sci. 2022 Sep;322:111359. doi: 10.1016/j.plantsci.2022.111359. Epub 2022 Jun 20. PMID: 35738478.Balfagon et al. (2018). Involvement of ascorbate peroxidase and heat shock proteins on citrus tolerance to combined conditions of drought and high temperatures. Plant Physiol Biochem. 2018 Jun;127:194-199. doi: 10.1016/j.plaphy.2018.03.029.McLoughlin et al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.Janicka-Russak et al. (2013). Modification of plasma membrane proton pumps in cucumber roots as an adaptation mechanism to salt stress. J Pant Physiol. March 14.Janicka-Russak et al. (2012). Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots. J. Exp. Botany, March 2012, ahead of print.
Hsp101/ClpB is a member of HSP100 protein family. These proteins help dissociate protein aggregates formed during heat stress to allow them to be refolded by other chaperones.Besides expression during heat stress, members of HSP100 protein family are also expressed during seed development. Hsp101 protein is both nuclear- and cytoplasmic-localized (1, 2).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized at -20 °C; once reconstituted to a final volume this antibody can be kept in 4°Cfor up to one year, in smaller portions to avoid contamination. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This antibody will not recognize any other cereal hsp101
Application Details:
1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
101 kDa
Not reactive in:
Arabidopsis thaliana
Selected references:
Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)Holding (2011). Pyrophosphate dependent fructose-6-phosphate 1-phosphotransferaseinduction and attenuation of Hsp gene expression duringendosperm modification inQualityProteinMaize. Plant Physiol Dec. 8 (ahead of print).Nieto-Sotelo et al. (2002). Maize HSP101 plays important roles in both induced and basal thermotolerance and primary root growth. Plant Cell 14: 1621-1633.Nieto-Sotelo et al. (1999). Characterization of a maize heat-shock protein 101 gene, HSP101, encoding a ClpB/Hsp100 protein homologue. Gene 230: 187-195.
Fructose-1,6 bisphosphate aldolase (ALD) is an enzyme catalazying a key reaction of glycolysis and energy production, converting D-fructose- 1,6-bisphospate into dihydroxyacetone phosphate and D-glyceraldehyde-3-phosphate. This enzyme is present in plant and animal tissues. Plant enzyme is a class I aldolase which does not require a bivalent metal cofactor. It is located to outer mitochondrial membrane.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This product can be sold containing ProClin if requested
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
38 | 38 kDa
Not reactive in:
Synechocystis sp.
Selected references:
Wang et al. (2018). iTRAQ-based quantitative proteomics analysis of an immature high-oleic acid near-isogenic line of rapeseed. Molecular Breeding January 2018, 38:2.Kamies et al. (2017). A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef. Proteomes. 2017 Nov 15;5(4). pii: E32. doi: 10.3390/proteomes5040032.Foley et al. (2017). A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate.Dev Cell. 2017 Apr 24;41(2):204-220.e5. doi: 10.1016/j.devcel.2017.03.018.Parveen et al. (2016). Chickpea Ferritin CaFer1 Participates in Oxidative Stress Response, and Promotes Growth and Development. Sci Rep. 2016 Aug 9;6:31218. doi: 10.1038/srep31218.Yam et al. (2016). Characterization of the Plasmodium Interspersed Repeats (PIR) proteins of Plasmodium chabaudi indicates functional diversity. Sci Rep. 2016 Mar 21;6:23449. doi: 10.1038/srep23449.Dixit (2015). Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves. Sci Rep. 2015 Nov 10;5:16205. doi: 10.1038/srep16205.Vera-Estrella et al. (2014). Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea. J Proteomics. 2014 Jun 2. pii: S1874-3919(14)00288-7. doi: 10.1016/j.jprot.2014.05.018.
Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN1 is highly abundant in the vascular elements of roots nodules, flowers and fruits, functioning in the assimilation of ammonium and the biosynthesis of glutamine for nitrogen transport. GLN2 is encoded by a single gene and is highly abundant in leaf mesophyll chloroplasts. Here GLN functions in the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide derived from a wide range of available sequences including all isoforms of Arabidopsis thaliana GLN1-1,1-2,1-3 and 1-4, (At5g37600, At1g66200, At3g17820, At5g16570)
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Maresca et al. (2021) Biological responses to heavy metal stress in the moss Leptodictyum riparium (Hedw.) Warnst. Ecotoxicol Environ Saf. 2022 Jan 1;229:113078. doi: 10.1016/j.ecoenv.2021.113078. Epub 2021 Dec 17. PMID: 34929502.Silva et al. (2019). Characterization of plant glutamine synthetase S-nitrosation. Nitric Oxide. 2019 Apr 23;88:73-86. doi: 10.1016/j.niox.2019.04.006.Wang et al. (2018). Response of Gracilaria lemaneiformis to nitrogen deprivation. Algal Research Volume 34, September 2018, Pages 82-96.Witzel et al. (2017). Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome. J Proteomics. 2017 Oct 3;169:215-224. doi: 10.1016/j.jprot.2017.04.008.Silva et al. (2015). Possible role of glutamine synthetase of the prokaryotic type (GSI-like) in nitrogen signaling in Medicago truncatula. Volume 240, November 2015, Pages 98–108.
Special application note:
The antibody will recognize both, cytoplasmic and chloroplastic forms of the GS enzyme
Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN2 is encoded by a single gene and is highly abundant in mesophyll cells of leaves for the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts. GLN2 is a target for thioredoxin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Brassica napus, Diplotaxis tenuifolia,Eruca versicaria, Glycine max, Hordeum vulgare, Medicago truncatula, Pinus sylvestris, Phaseolus vulgaris, Physcomitrium patens, Populus sp., Triticum aestivum, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide which is a part of part of the glutamine synthetase/guanido kinase superfamily catalytic region chosen from various available sequences, including Arabidopsis thaliana GLN2, UniProt: Q43127, TAIR: AT5G35630
Hertle et al. (2021). Horizontal genome transfer by cell-to-cell travel of whole organelles. Science Advances. Volume 7. 01 Jan 2021 : eabd8215 DOI: 10.1126/sciadv.abd8215Ancin et al. (2021) Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco. J Exp Bot. 2021 Jun 22;72(13):4949-4964. doi: 10.1093/jxb/erab193. PMID: 33963398; PMCID: PMC8219043.Chen et al. (2018). TIC236 links the outer and inner membrane translocons of the chloroplast. Nature. 2018 Dec;564(7734):125-129. doi: 10.1038/s41586-018-0713-y.Tamburino et al. (2017). Chloroplast proteome response to drought stress and recovery in tomato (Solanum lycopersicum L.). BMC Plant Biol. 2017 Feb 10;17(1):40. doi: 10.1186/s12870-017-0971-0.Dixit (2015). Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves. Sci Rep. 2015 Nov 10;5:16205. doi: 10.1038/srep16205.
Special application note:
This product can be sold contacining proclin if requested
CSP41b (CRB, RAP38 ortholog, gb5f) is a chloroplast stem-loop-binding, ribosome-associated endonuclease. This protein is involved in 23S rRNA metabolism and highly conserved in photosynthetic organisms including angio- and gymnosperms, green algae, and cyanobacteria.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).Beligni & Mayfield (2008). Arabidopsis thaliana mutants reveal a role for CSP41a and CSP41b, two ribosome-associated endonucleases, in chloroplast ribosomal RNA metabolism. Plant Mol Biol. 67:389-401.Hassidim et al. (2007). Mutations in CHLOROPLAST RNA BINDING provide evidence for the involvement of the chloroplast in the regulation of the circadian clock in Arabidopsis. Plant J. 51:551-562.
PEB is an extraction buffer for disruption and solubilisation of total protein from plant tissue and algal cells. The use of the anionic detergent LDS together with the recommended procedure (combination of sonication and freeze/thaw cycles) has been shown to increase the number of solubilised and non-degraded proteins when compared to other methods of cell disruption (see reference). The estimated hands-on time for the recommended procedure is 20-30 minutes for 1-2 samples. Expected yields will be 1.5-6 g/ l total protein (recovered from standard procedure) depending on the starting material, e.g. its biological stage, homogenization method used (bead beater vs. sonication).
Product Type:
Antibody
Storage Temp:
Stable at RT for at least 1 month; short-term storage (6 monthss) at 4°Cand long term storage (1 year or more) at -20 °C.
Species Reactivity:
PEB has been tested on a wide range of species and tissues from Higher plants, Mosses, Llichens, Algae, Diatoms, Dinoflagellates, Cyanobacteria. Extracts may be quantified using detergent (LDS) compatible methods, and have been shown to give highly reproducible and quantitative results in subsequent SDS PAGE gel electrophoresis, Western blotting, and Immunoprecipitation (IP). Most of Agrisera commercial antibodies are tested on plant or algal samples extracted with this buffer. An example can be found here.
5 x 2 ml (4x stock) allows up to 75 isolations of plant material (using 500 µl 1x PEB for 100 mg fresh weight) or 190 isolations of algal material (using 200 µl 1x PEB for cell amounts corresponding to 4-10 µg total chlorophyll)
Selected references:
Altuntas et al. (2020). Proline-stimulated signaling primarily targets the chlorophyll degradation pathway and photosynthesis associated processes to cope with short-term water deficit in maize. Photosynth Res. 2020 Apr;144(1):35-48. doi: 10.1007/s11120-020-00727-w.P rez-L pez et al. (2020). Transcriptome Analysis Identifies Plasmodiophora brassicae Secondary Infection Effector Candidates. J Eukaryot Microbiol. 2020 Jan 11. doi: 10.1111/jeu.12784.Morin et al. (2019). Morin et al. (2019). Response of the sea-ice diatom Fragilariopsis cylindrus to simulated polar night darkness and return to light. Limnology and Oceanography. 9999, 2019, 1â??20. (sea-ice diatom)Bausch, A.R., Juhl, A.R., Donaher, N.A. et al. Mar Biol (2019) 166: 80.Matsuo and Atsumi (2018). Xylosylation of proteins by expression of human xylosyltransferase 2 in plants. J Biosci Bioeng. 2018 Sep;126(3):371-378. doi: 10.1016/j.jbiosc.2018.03.013.Brouwer et al. (2011) TheImpact ofLightIntensity onShade-InducedLeaf Senescence. Plant Cell Environ. Dec. 15 (ahead of print).Kosawang et al. (2011) Hydrogen yield from a hydrogenase in Frankia R43 at different levels of the carbon source propionate. Journal of Environmental Management, Jan 26
Special application note:
Buffer components (4x): contains ~ 40% v/v glycerol [HOCH2CH(OH)CH2OH], Tris-HCl [NH2C(CH2OH)3 HCl] pH 8.5, LDS [CH3(CH2)11OSO3Li], EDTA [(HO2CCH2)2NCH2CH2N(CH2CO2H)2]It is recommended to include a protease inhibitor (not supplied with this buffer) from a freshly made stock while preparing the ready-to-use 1x PSB.PEB has been optimized for quantitative small-scale preparation of whole protein extracts from plant/algal tissue. Extraction using the procedure described below will result in maximum yield of proteins and diminish protein degradation and aggregation.Extracts may be quantified using detergent (LDS) compatible methods and have been shown to give highly reproducible and quantitative results in subsequent SDS PAGE gel electrophoresis, Western Blotting, and immunoprecipitation.PEB has been tested on a wide range of species and tissues from higher plants, mosses, lichens, algae, diatoms, dinoflagellates, and cyanobacteria.
Nucleoside diphosphate kinase protein (EC=2.7.4.6) is catalysing the transfer of a g-phosphate group from adenosine triphosphate (ATP) to a cognate nucleoside diphosphate. This contributes to balancing of the nucleoside pool. NDPK enzymes are present in most subcellular compartments of the eukaryotic cell.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Pisum sativum
Expected Species:
Brassica campestris, Oryza sativa, Spinacia oleracea, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Pisum sativum NDPK, UniProt: Q9SP13. Peptide is conserved in Arabidopsis thaliana NDPKIII, UniProt:O49203 and NDPK IV, UniProt: Q8LAH8, but is not conserved in NDPK1.
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Dicots and (including Lilium superbum) chloroplast AtpA; may cross-react with mitochondrial AtpA; Algae, Nannochloropsis gaditana, CyanobacteriaSpecies of your interest not listed? Contact us
Immunogen:
Recombinant maize chloroplast AtpA P05022
Applications:
Immunogold (IG), Immunoprecipitation (IP), Western blot (WB)
Pao et al. (2018). Lamelloplasts and minichloroplasts in Begoniaceae: iridescence and photosynthetic functioning. J Plant Res. 2018 Mar 2. doi: 10.1007/s10265-018-1020-2. (ImmunoGold)Zhang et al. (2017). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989.Jeon et al. (2017). Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Plant Mol Biol. 2017 Nov;95(4-5):463-479. doi: 10.1007/s11103-017-0664-y.Murcha et al. (2016). Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016.Camejo et al. (2015). Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits. Proteomics. 2015 Aug;15(15):2634-42. doi: 10.1002/pmic.201400370.Yang et al. (2015). Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum. Protein Expr Purif. 2015 May 12. pii: S1046-5928(15)00111-4. doi: 10.1016/j.pep.2015.05.006.
Special application note:
Sequence of the protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757
PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Sorghum bicolor, Zea mays
Expected Species:
Hordeum vulgare, Oryza sativa Species of your interest not listed? Contact us
Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotic tissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide. Its function is labeling of proteins for degradation through ubiquitin proteasome system (UPS).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Antibody can be used to check qubiquitination status in the whole plant extracts.Technical note: It is very difficult to detect ubiquitin monomers in total cell extracts due to a great abundance of poly and multi-ubiquitinated proteins. Recommended is size separation of protein extracts before gel electrophoresis focused on good resolution of region between 6-10 kDa.Suggested extraction buffer: 100 mM Tris-HCl, pH 8.0, 0.1% [w/v] SDS, 0.5% [w/v] sodium deoxycholate, 1% [v/v] glycerol, 50 mM sodium metabisulfite, 20���mM N-ethylmaleimide (NEM) and protease inhibitor cocktail (Roche) (Orosa et al. 2018). This buffer will help to stabilize the conjugates and will help to detect any increase or decrease in conjugate accumulation using the antibodies.
Application Details:
1 : 10 000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
8,5 kDa
Not reactive in:
Algae
Selected references:
Jeran et al. (2021) The PUB4 E3 Ubiquitin Ligase Is Responsible for the Variegated Phenotype Observed upon Alteration of Chloroplast Protein Homeostasis in Arabidopsis Cotyledons. Genes (Basel). 2021 Sep 6;12(9):1387. doi: 10.3390/genes12091387. PMID: 34573369; PMCID: PMC8464772.Filippi et al. (2019). Caspase-3-like activity and proteasome degradation in grapevine suspension cell cultures undergoing silver-induced programmed cell death. J Plant Physiol. 2019 Feb;233:42-51. doi: 10.1016/j.jplph.2018.12.003.Krasuska et al. (2016). Nitric oxide-polyamines cross-talk during dormancy release and germination of apple embryos. Nitric Oxide. 2016 Nov 23. pii: S1089-8603(16)30178-1. doi: 10.1016/j.niox.2016.11.003. [Epub ahead of print]
Special application note:
Ubiquitin mutant is lethal, therefore could not be used in validation of this antibody.
SUMO1 - Small Ubiquitin-like Modifier ubiquitin like protein binds in reversible way to various protein targets and plays a role as a signaling regulator.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Glycine max, Nicotiana tabacum, Picea sitchensis, Pisum sativum, Populus trichocarpa, Solanum lycopersicum, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
Recombinant proSUMO1 from Arabidopsis thaliana Q547B9, At4g26840 with a his tag
Antibodies will also detect SUMO2 protein. Suggested extraction buffer: 100 mM Tris-HCl, pH 8.0, 0.1% [w/v] SDS, 0.5% [w/v] sodium deoxycholate, 1% [v/v] glycerol, 50 mM sodium metabisulfite, 20 mM N-ethylmaleimide (NEM) and protease inhibitor cocktail (Roche) (Orosa et al. 2018). This buffer will help to stabilize the conjugates and will help to detect any increase or decrease in conjugate accumulation using the antibodies.
Application Details:
1 : 1000-1 : 5000 (WB)
Purity:
Total IgG. Protein G purified in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
10,97 | 12 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Szadeczky-Kardoss et al. (2022) Elongation factor TFIIS is essential for heat stress adaptation in plants. Nucleic Acids Res. 2022 Feb 28;50(4):1927-1950. doi: 10.1093/nar/gkac020. PMID: 35100405; PMCID: PMC8886746.Colignon et al. (2019). Dual coordination of the SUMOylation and phosphorylation pathways during the response to heat stress in Solanum tuberosum. Environmental and Experimental Botany Volume 162, June 2019, Pages 192-200.Rosa et al. (2018). Insights into the transcriptional and post-transcriptional regulation of the rice SUMOylation machinery and into the role of two rice SUMO proteases. BMC Plant Biol. 2018 Dec 12;18(1):349. doi: 10.1186/s12870-018-1547-3.Guo et al. (2017). Sumoylation stabilizes RACK1B and enhance its interaction with RAP2.6 in the abscisic acid response. Sci Rep. 2017 Mar 8;7:44090. doi: 10.1038/srep44090.Tomanov et al. (2014). Arabidopsis PIAL1 and 2 Promote SUMO Chain Formation as E4-Type SUMO Ligases and Are Involved in Stress Responses and Sulfur Metabolism. Plant Cell. 2014 Nov;26(11):4547-60. doi: 10.1105/tpc.114.131300.
S1 ribosomal protein is involved in initiation of translation by recognition and binding of mRNAs through association with the 30S ribosomal subunit. S1 protein is localized in bacterial cytosol.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Carrieri et al. (2021) Overexpression of NblA decreases phycobilisome content and enhances photosynthetic growth of the cyanobacterium Synechococcus elongatus PCC 7942,Algal Research,Volume 60, 2021, 102510, ISSN 2211-9264, https://doi.org/10.1016/j.algal.2021.102510.Zav?el et al. (2019). Quantitative insights into the cyanobacterial cell economy. Elife. 2019 Feb 4;8. pii: e42508. doi: 10.7554/eLife.42508.Koskinen et al. (2018). Inactivation of group 2 ? factors upregulates production of transcription and translation machineries in the cyanobacterium Synechocystis sp. PCC 6803. Sci Rep. 2018 Jul 9;8(1):10305. doi: 10.1038/s41598-018-28736-9.Kurkela et al. (2017). Acclimation to High CO2 Requires the ? Subunit of the RNA Polymerase in Synechocystis. Plant Physiol. 2017 May;174(1):172-184. doi: 10.1104/pp.16.01953. Epub 2017 Mar 28.Plominsky et al. (2013). Dinitrogen Fixation Is Restricted to the Terminal Heterocysts in the Invasive Cyanobacterium Cylindrospermopsis raciborskii CS-505. PLOS ONE, Open Access.
Assimilatory nitrate reductase (NR), (EC.1.6.6.1) catalyses the reduction of nitrate to nitrite in the cytoplasm. Plants contain 2 forms of NR: NADH-NR (most common form in plants and algae, predominantly found in green tissues) and NAD(P)H-NR (uses NADH or NADPH as the electron donor, constitutively expressed in plants at a low level). NADH-NR is a homodimer of two identical subunits (100-115 kDa each, hold together by a Mo-cofactor) each of them coded by up to three genes (NR1-3, NIA1-NIA3).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide derived from conserved domain in NADH-NR protein sequences including A.thaliana NR1 P11832, At1g77760 and NR2 P11035, At1g37130
In Chlamydmonas reinhardtii anti-NR antibody is also reacting with L-Aminoacid Oxidase (a nitrogen scavenging enzyme induced during nitrogen starvation).Using this antibody genome editing in Chlorella vulgaris UTEX395 by CRISPR-Cas9 system has been demonstrated as described in Kim et al. (2021)Chemiluminescent detection is advised for NR detection using this antibody.
Costa-Broseta et al. (2021). Post-Translational Modifications of Nitrate Reductases Autoregulates Nitric Oxide Biosynthesis in Arabidopsis. Int J Mol Sci. 2021 Jan 7;22(2):E549. doi: 10.3390/ijms22020549. PMID: 33430433.Kim et al. (2021). Establishment of a Genome Editing Tool Using CRISPR-Cas9 in Chlorella vulgaris UTEX395. Int J Mol Sci. 2021 Jan 6;22(2):E480. doi: 10.3390/ijms22020480. PMID: 33418923.Prinsi et al. (2021). Biochemical and Proteomic Changes in the Roots of M4 Grapevine Rootstock in Response to Nitrate Availability. Plants 10, no. 4: 792. https://doi.org/10.3390/plants10040792Maresca et al. (2021) Biological responses to heavy metal stress in the moss Leptodictyum riparium (Hedw.) Warnst. Ecotoxicol Environ Saf. 2022 Jan 1;229:113078. doi: 10.1016/j.ecoenv.2021.113078. Epub 2021 Dec 17. PMID: 34929502.Zhang et al. (2020). Hydrogen sulfide and rhizobia synergistically regulate nitrogen (N) assimilation and remobilization during N deficiency-induced senescence in soybean. Plant Cell Environ. 2020 Feb 3. doi: 10.1111/pce.13736.
ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex. Alternative name of gamma subunit is also: F-ATPase gamma subunit.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Apparent molecular weight of subunit gamma (and as general rule most of ATP synthase subunits) is quite different between Chlamydomonas (42 kDa) and higher plants (38 kDa in spinach), see figure in Lemaire et al. (1989).
Pipitone et al. (2021). A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis. Elife. 2021 Feb 25;10:e62709. doi: 10.7554/eLife.62709. PMID: 33629953; PMCID: PMC7906606.Storti et al. (2020). The activity of chloroplast NADH dehydrogenase-like complex influences the photosynthetic activity of the moss Physcomitrella patens. doi.org/10.1101/2020.01.29.924597Pralon et al. (2019). Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019 Jun 20;2:220. doi: 10.1038/s42003-019-0477-4.Li et al. (2019). A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nat Genet. 2019 Apr;51(4):627-635. doi: 10.1038/s41588-019-0370-6.Liang et al. (2018). Thylakoid-Bound Polysomes and a Dynamin-Related Protein, FZL, Mediate Critical Stages of the Linear Chloroplast Biogenesis Program in Greening Arabidopsis Cotyledons. Plant Cell. 2018 Jul;30(7):1476-1495. doi: 10.1105/tpc.17.00972. Epub 2018 Jun 7.
Special application note:
This product can be sold containing ProClin if requested
Curvature thylakoid 1A (CURT1A) belongs to a protein family, conserved in plants and cyanobaceria. There are four Arabidopsis thaliana CURT1 proteins: CURT1A,B,C and D. It is proposed that CURT1 proteins modify thylakoid architecture by inducing membrane curvature at grana margins.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Pisum sativum
Expected Species:
Nicotiana tabacum, Zea maysSpecies of your interest not listed? Contact us
Nishioka et al. (2021). Phos-tag-based approach to study protein phosphorylation in the thylakoid membrane. Photosynth Res. 2021 Jan;147(1):107-124. doi: 10.1007/s11120-020-00803-1. Epub 2020 Dec 2. PMID: 33269435; PMCID: PMC7728655.Fukura et al. (2021) Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions. J Plant Physiol. 2021 Nov;266:153535. doi: 10.1016/j.jplph.2021.153535. Epub 2021 Sep 25. PMID: 34607178.Liang et al. (2018). Thylakoid-Bound Polysomes and a Dynamin-Related Protein, FZL, Mediate Critical Stages of the Linear Chloroplast Biogenesis Program in Greening Arabidopsis Cotyledons. Plant Cell. 2018 Jul;30(7):1476-1495. doi: 10.1105/tpc.17.00972. Epub 2018 Jun 7.Armbruster et al. (2013). Arabidopsis CURVATURE THYLAKOID1 Proteins Modify Thylakoid Architecture by Inducing Membrane Curvature. 2013 Jul;25(7):2661-78. doi: 10.1105/tpc.113.113118. Epub 2013 Jul 9.
14.3.3’s are 30KDa proteins involved in protein interactions with target proteins containing phosphorylated target sites. Functions of 14.3.3’s include acting as adaptors or scaffolds, stimulating protein-protein interaction, altering target protein activity, causing conformational changes of target proteins, regulating subcellular localisation and also facilitating transport (for example nuclear import/export and transport in the endomembrane system). A variety of apparently unrelated biological activities, including a role in development and signal transduction have been ascribed to the 14.3.3 family. To date, five barley 14.3.3 homologues have been identified and characterised and named 14.3.3A through E.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Hordeum vulgare
Expected Species:
Horderum vulgare
Immunogen:
synthetic peptide specific to the barley C isoform, conjugated to KLH
Molecular interactions between wall polysaccharides, which include cellulose and a range of non-cellulosic polysaccharides such as xyloglucans and (1,3;1,4)-?-d-glucans, are fundamental to cell wall properties. These interactions have been assumed to be non-covalent in nature in most cases. A highly purified barley xyloglucan xyloglucosyl transferase HvXET5 (EC 2.4.1.207), a member of the GH16 group of glycoside hydrolases, catalyses the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates, and between xyloglucans and (1,3;1,4)-?-d-glucans. It is possible that XETs could link different polysaccharides in vivo, and hence influence cell wall strength, flexibility and porosity.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Hordeum vulgare, Oryza sativa
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Two synthetic peptides from highly conserved region of Horderum vulgare XTH-Xet
Tsuchiya et al. (2015). Distribution of XTH, expansin, and secondary-wall-related CesA in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum). Front Plant Sci. 2015 May 15;6:323. doi: 10.3389/fpls.2015.00323.Liu et al. (2013). Brittle Culm1, a COBRA-Like Protein, Functions in Cellulose Assembly through Binding Cellulose Microfibrils. PLoS Genet 9(8): e1003704. doi:10.1371/journal.pgen.1003704 (Oryza sativa, western blot)Hrmova et al. (2007) A barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates and (1,3;1,4)-β. J. Biol. Chem. 82: 12951-12962.
Hordeum vulgare Pht1-6 is a putative low-affinity barley phosphate transporter that is likely to function in phosporus remobilisation around the plant (expressed in plant vascular tissues).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Hordeum vulgare
Immunogen:
two synthetic peptides from the loop region of Pht1-6 (Q8H6D9), conjugated to KLH
Hordeum vulgare Pht1-1 and 1-2 are putative high-affinity barley phosphate transporters that are likely to function in phosphate uptake from the soil (expressed in root epidermal cells).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Hordeum vulgare
Expected Species:
Triticum aestivumSpecies of your interest not listed? Contact us
Immunogen:
two synthetic peptides from the loop region of Pht1-1 (Q8H6E0) and 1-2, conjugated to KLH
Applications:
ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
Antibody cannot discriminate between Pht1-1 or Pht1-2 due to the high level of sequence conservation
Application Details:
1 : 10 000 (ELISA), 1: 100 (IL), 1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
56.9 kDa
Not reactive in:
Arabidopsis thaliana
Selected references:
Namyslov et al. (2020). Exodermis and Endodermis Respond to Nutrient Deficiency in Nutrient-Specific and Localized Manner. Plants (Basel). 2020 Feb 6;9(2). pii: E201. doi: 10.3390/plants9020201. (immunolocalization)
PsaE is a nucleus encoded subunit of the Photosystem I reaction center. It is located on the stroma side and interacts with PsaF. PsaE may be involved in Fd reduction.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Hordeum vulgare
Expected Species:
Chlamydomonas reinhardtii, Chlorella, Oryza sativa, Populus canadensis, Solanum lycopersicum, Spinacia oleracea, Zea mays Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from PsaE N-terminal part, conserved in di and monocots and some green algae PsaE protein (not Chlamydomonas), including Arabidopsis thaliana PSI-E A Q9S831, At4g28750 and PSI-E B Q9S714, At2g20260
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Simakawa et al. (2020). Near-infrared in Vivo Measurements of Photosystem I and Its Lumenal Electron Donors With a Recently Developed Spectrophotometer. Photosynth Res. , 144 (1), 63-72 Li et al. (2018). Modulating plant growth-metabolism coordination for sustainable agriculture. Nature. 2018 Aug 15. doi: 10.1038/s41586-018-0415-5.Yang et al. (2017). Tetratricopeptide repeat protein Pyg7 is essential for photosystem I assembly by interacting with PsaC in Arabidopsis. Plant J. 2017 Jun 21. doi: 10.1111/tpj.13618.
The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It is a small GTPase that undergoes a GDP/GTP nucleotide exchange cycle and it is an important regulator of cellular trafficking.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
References describing immunolocalization (IF) and (IG) studies:Pimpl et al (2000). In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles. Plant Cell. 2000 Nov;12(11):2219-36.Ritzenthaler et al. (2002). Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera. Plant Cell. 2002 Jan;14(1):237-61.
Application Details:
1 : 1000 (IF), 1 : 100 (IG), 1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
21 kDa (Arabidopsis thaliana)
Not reactive in:
Microsporidia sp.
Selected references:
Farago et al. (2022) Small paraquat resistance proteins modulate paraquat and ABA responses and confer drought tolerance to overexpressing Arabidopsis plants. Plant Cell Environ. 2022 Jul;45(7):1985-2003. doi: 10.1111/pce.14338. Epub 2022 Apr 29. PMID: 35486392; PMCID: PMC9324991.Narasimhan et al. (2021) Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking. Plant Physiol. 2021 Mar 18:kiab134. doi: 10.1093/plphys/kiab134. Epub ahead of print. PMID: 33734402.Hurny et al. (2020). SYNERGISTIC ON AUXIN AND CYTOKININ 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance. Nat Commun. 2020 May 1;11(1):2170. doi: 10.1038/s41467-020-15895-5. (immunolocalization)Kuang et al. (2019). Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins. Front Plant Sci. 2019 May 15;10:641. doi: 10.3389/fpls.2019.00641. eCollection 2019.Singh et al. (2018). A single class of ARF GTPase activated by several pathway-specific ARF-GEFs regulates essential membrane traffic in Arabidopsis. PLoS Genet. 2018 Nov 15;14(11):e1007795. doi: 10.1371/journal.pgen.1007795.
Special application note:
Cellular [compartment marker] of Golgi in immunolocalization and COP1 in western blot
Sar1 belongs to a small GTPase superfamily and GTP binging activity. This protein is involved in intracellular protein transport. There are two different non-clathrin-coated vesicles that are responsible for transport between ER and the Golgi. Coat protein complexes involving membrane-associated GTP binging proteins – Arf1 and Sar1p for COPI and COPII are needed for formation of COP-coated vesicles. Sar1p is a cytosolic protein. It is temporarily recruited onto the membranes of the ER.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Immunolocalization method with Sar1 antibodies is described in: Yao-dong Yang (2005). Dynamics of COPII vesicles and the Golgi apparatus in cultured Nicotiana tabacum BY-2 cells provides evidence for transient association of Golgi stacks with endoplasmic reticulum exit sites. Plant Cell. 2005 May;17(5):1513-31. Epub 2005 Apr 1.
Application Details:
1 : 50 (IG), 1 : 500 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
21 kDa (Arabidopsis thaliana)
Not reactive in:
Nicotiana tabacum
Selected references:
Shen et al. (2014). The fronds tonoplast quantitative proteomic analysis in arsenic hyperaccumulator Pteris vittata L. J Proteomics. 2014 Feb 4. pii: S1874-3919(14)00047-5. doi: 10.1016/j.jprot.2014.01.029.Liu et al. (2014). SCFSLF-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida. Front. Genet., 22 July 2014 | doi: 10.3389/fgene.2014.00228
Special application note:
For immunogold experiments plant tissue has been fixed with GA in PFA/PIPES. LR White resin has been used. Tested species were: Triticum aestivum, Panicum miliaceum, Panicum maximum, Echinochloa crus-galli Eragrostis neomexicana, Digitaria sanguinalis. Publication in preparation.
Sec21p is a constituent of the COPI vesicle coatomer.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This antibody can be used as a Golgi marker in immunolocalization and as a marker of COP1 in Western blot.References describing immunolocalization (IF) and (IG) studies:Pimpl et al (2000). In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles. Plant Cell. 2000 Nov;12(11):2219-36. Ritzenthaler et al. (2002). Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera. Plant Cell. 2002 Jan;14(1):237-61.
Application Details:
1 : 1000 (IF), 1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
98 kDa
Not reactive in:
Nicotiana tabacum, Microsporidia sp.
Selected references:
Hurny et al. (2020). SYNERGISTIC ON AUXIN AND CYTOKININ 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance. Nat Commun. 2020 May 1;11(1):2170. doi: 10.1038/s41467-020-15895-5. (immunolocalization)Lupette et al. (2019). The architecture of lipid droplets in the diatom Phaeodactylum tricornutum. Algal Research Volume 38, March 2019, 101415.Singh et al. (2018). A single class of ARF GTPase activated by several pathway-specific ARF-GEFs regulates essential membrane traffic in Arabidopsis. PLoS Genet. 2018 Nov 15;14(11):e1007795. doi: 10.1371/journal.pgen.1007795.Kitakura et al. (2017). BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana. Plant Cell Physiol. 2017 Oct 1;58(10):1801-1811. doi: 10.1093/pcp/pcx118.Nagel et al. (2017). Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017 Aug 7. pii: 201710866. doi: 10.1073/pnas.1710866114.Wang et al. (2016). Comprehensive proteomic analysis of developing protein bodies in maize (Zea mays) endosperm provides novel insights into its biogenesis. J Exp Bot. 2016 Dec;67(22):6323-6335. Epub 2016 Oct 27.Wattelet-Boyer et al. (2016). Enrichment of hydroxylated C24- and C26-acyl- chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains. Nat Commun. 2016 Sep 29;7:12788. doi: 10.1038/ncomms12788.Derbyshire et al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314.Tanaka et al. (2013). Cell Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments in Arabidopsis thaliana. PLoS Genet. May;9(5). (immunolocalization).Hopff et al. (2013). The plasma membrane proteome of maize roots grown under low and high iron conditions. J Proteomics Jan 24.Pimpl et al (2000). In situ localization and in vitro induction of plant COPI-coated vesicles. Plant Cell. 2000 Nov;12(11):2219-36.
Special application note:
This product can be sold containing proclin if requested
Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein P05067
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Human
Expected Species:
Bovine, Chicken, Dog, Porcine, Rabbit
Immunogen:
synthetic peptide chosen from human Abeta (1-42) protein, Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V-I-A
Applications:
Dot Blot (Dot), ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
The antibody can detect Abeta (1-42), Abeta (1-28) Abeta (1-20) and Abeta (1-17), This product exhibits a low reactivity to monomeric Abeta (1-42) as determined by SDS-PAGE and Western blotting,Immunolocalization: human tissue was paraffin-embedded and sectioned, De-waxed and rehydrated in an ethanol gradient, Antigens were retrieved in sodium citrate buffer (pH 6) at 95 C for 1 h, The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit
Application Details:
1 : 1000 (DB), 1 : 3000 (ELISA), 1-2 l/ml (IL)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
4,5 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Lindhagen-Persson et al. (2010). Amyloid-β oligomer specificity mediated by the IgM isotype--implications for a specific protective mechanism exerted by endogenous auto-antibodies. PLoS One. 2010 Nov 10;5(11):e13928. doi: 10.1371/journal.pone.0013928.
Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein P05067
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4 C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Human
Expected Species:
Bovine, Chicken, Dog, Porcine, Rabbit
Immunogen:
synthetic peptide chosen from human Abeta (1-42) protein, Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V-I-A
Rieske Iron-Sulfur Protein (Q9ZR03) is located in chloroplast thylakoid membrane as a component of cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. Alternative names: Rieske iron-sulfur protein, RISP, ISP, plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein, proton gradient regulation protein 1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Pipitone et al. (2021). A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis. Elife. 2021 Feb 25;10:e62709. doi: 10.7554/eLife.62709. PMID: 33629953; PMCID: PMC7906606.Kana et al. (2020). Fast Diffusion of the Unassembled PetC1-GFP Protein in the Cyanobacterial Thylakoid Membrane. Life (Basel). 2020 Dec 29;11(1):E15. doi: 10.3390/life11010015. PMID: 33383642.Zhang et al. (2020). Enhanced Relative Electron Transport Rate Contributes To Increased Photosynthetic Capacity In Autotetraploid Pak Choi. Plant Cell Physiol. 2020 Jan 6. pii: pcz238. doi: 10.1093/pcp/pcz238.Pralon et al. (2019). Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019 Jun 20;2:220. doi: 10.1038/s42003-019-0477-4. Koochak et al. (2019). The structural and functional domains of plant thylakoid membranes. Plant J. 2019 Feb;97(3):412-429. doi: 10.1111/tpj.14127.
Special application note:
This product can be sold containing Proclin if requested.
Rieske Iron-Sulfur Protein (Q9ZR03)is located in chloroplast thylakoid membrane as a component of cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. Alternative names: Rieske iron-sulfur protein, RISP, ISP, plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein, proton gradient regulation protein 1This is a recombinant protein standard, source: Synechocystis PCC 6803.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Concentration: after adding 225 l of milliQ water final concentration of the standard is 0.15 pmol/ lProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:
Standard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2μg of chlorophyll will give a PsbA signal in this range.Positive control:a 2μl load per well is optimal for most chemiluminescent detection systems.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:
For reconstitution add 225 l of milliQ water, Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized
Molecular Weight:
33 kDa (larger than native protein due to the addition of His-tag), In most gel systems, PetC protein migrates at 23 kDa
Selected references:
Pipitone et al. (2021). A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis. Elife. 2021 Feb 25;10:e62709. doi: 10.7554/eLife.62709. PMID: 33629953; PMCID: PMC7906606.Li et al. (2014). The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytol. 2014 Sep 25. doi: 10.1111/nph.13037.Wu et al. (2014). Large centric diatoms allocate more cellular nitrogen to photosynthesis to counter slower RUBISCO turnover rates. Front. Mar. Sci., 09 December 2014 | doi: 10.3389/fmars.2014.00068.
Special application note:
The PetC protein standard can be used in combination with global anti-PetC antibodies to quantitate PetC from a wide range of species. Global antibodies are raised against highly conserved amino acid sequences in the PetC protein.Quantitative western blot: detailed method description, video tutorial
Ribosomal protein L-30 is a part of 50S ribosomal large subunit, synthesized in chloroplast. Schmidt et al. (1983) Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas. J Cell Biol 96(5):1451-1463
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii, weakly reacts with the r-protein in spinach ca. 1 %
Immunogen:
Purified native Chlamydomonas reinhardtii L-30 protein eluted from a gel piece
Cross react with L2 and L26 proteins of Chlamydomonas reinhardtii. L7/L12 is very acidic, may not bind well to nitrocellulose membrane and can have abberant mobility depending upon conditions.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Name of this antibody has been changed from L-30 | 50S ribosomal protein L30 to L7/L12 based on the following reference: Randolph-Anderson et al. (1989). Electrophoretic and immunological comparisons of chloroplast and prokaryotic ribosomal proteins reveal that certain families of large subunit proteins are evolutionarily conserved. J Mol Evol. 1989 Jul;29(1):68-88.
Conglutin gamma, an oligomeric protein, is one of storage proteins of lupin seeds, called also lupin-specific globulin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Villa et al. (2020). Immunoreactivity of Lupine and Soybean Allergens in Foods as Aected by Thermal Processing. Foods. 2020 Feb 27;9(3). pii: E254. doi: 10.3390/foods9030254.Tomczak et al. (2019). Differences in the immunoreactivity of milk from local farms and from points of purchase. Eur Food Res Technol, Nov 2019.Foley et al. (2015). Analysis of conglutin seed storage proteins across lupin species using transcriptomic, protein and comparative genomic approaches. BMC Plant Biol. 2015 Apr 19;15:106. doi: 10.1186/s12870-015-0485-6.Czubiński et al. (2015). Digestion susceptibility of seed globulins isolated from different lupin species. European Food Research and Technology pp 1-13.
Pairing and synapsis of homologous chromosomes is required for normal chromosome segregation and the exchange of genetic material via recombination during meiosis. Synapsis is complete at pachytene following the formation of a tri-partite proteinaceous structure known as the synaptonemal complex (SC). In yeast, HOP1 is essential for formation of the SC, and localises along chromosome axes during prophase I. Homologues in Arabidopsis (AtASY1), Brassica (BoASY1) and rice (OsPAIR2) have been isolated through analysis of mutants that display decreased fertility due to severely reduced synapsis of homologous chromosomes. Analysis of these genes has indicated that they play a similar role to HOP1 in pairing and formation of the SC through localisation to axial/lateral elements of the SC.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Triticum aestivum
Expected Species:
Triticum aestivum
Immunogen:
KLH-conjugated synthetic pepitde chosen from Triticum aestivum ASY1 UniProt: A7TVU8
Total IgG. Protein G purified purified from Cell culture supernatant.
Reconstitution:
For reconstitution add 50 l of sterile water/tube
Molecular Weight:
66,3 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Lewandowska et al. (2021) The proteome of developing barley anthers during meiotic prophase I. J Exp Bot. 2021 Nov 10:erab494. doi: 10.1093/jxb/erab494. Epub ahead of print. PMID: 34758083.Darrier et al. (2019). Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High-Resolution Microscopy. Methods Mol Biol. 2020;2061:207-215. doi: 10.1007/978-1-4939-9818-0_15.
Lipoxygenases are a family of enzymes involved in oxidative stress response. They catalyse the oxygenation of polyunsaturated fatty acids to lipidhydroperoxides. LOX’s are known to play a role in plant growth and development.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Glycerol 1:1 can be added to improve the stability of this antibody.
Host Animal:
Mouse
Species Reactivity:
Triticum aestivum
Expected Species:
Hordeum vulgare, Oryza sativaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide chosen from Triticum aestivum LOX2 sequence
Total IgG. Protein G purified purified from Cell culture supernatant.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
90 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
LOX2 protein seems to be antigenically different compare to LOX isoforms isolated from leaves and flowers therefore this antibody being a monoclonal will not detect LOX2 from seeds, Brady et al (2014), Lipids: Structure and Function: The Biochemistry of Plants
Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. The wheat vernalization gene VRN-2 is a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN-2, whether by natural mutations or deletions, results in spring lines, which do not require vernalization to flower.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Triticum aestivum
Expected Species:
Triticum monococcumSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide chosen from Triticum aestivum VRN2 sequence
There is some non-specific binding in a western blot when using anti-TaVRN2 antibody on nuclear extracts of T, monococcum
Application Details:
1 : 10 000 (ELISA), 1 : 1000 (WB)
Purity:
Total IgG. Protein G purified purified from Cell culture supernatant.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
23,7 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 and ZCCT2 proteins were used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2 antibody was able to differentiate VRN-2 from the ZCCT2 protein.
Cytochrome c is located in inner mitochondrial membrane. It is a small heme protein which, unlike other cytochromes, is highly soluble. This protein is an essential component of the electron transport chain, where it undergoes oxidation and reduction without binding oxygen.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles and Store at -80°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
cytc1 and cytc2 from following species: A. theoprasi, Brassica napus, Brassica oleracea, Cannabis sativa, C. maxima, Chlamydomonas reinhardtii (peptide target partially conserved), Lupinus luteus, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Ostreococcus (peptide target partially conserved), P. aurea, Physcomitrium patens, Ricinus communis, S. nigra, Solanum lycopersivum, Vitis vinifera.Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana cytochrome c protein sequence, UniProt:D7KMK0 (C-1) D7LY03 (C-2), TAIR: At1g22840 (Cytc1) and At4g10040 (Cytc2)
The presence of cytochrome c in the cysotol is a marker of PCD (programmed cell death)
Application Details:
1: 100 (IL), 1 : 5000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
12.5 | 14 kDa (for Arabidopsis thaliana)
Not reactive in:
Arabidopsis thaliana CytC6, Chinesecuscuta sp.
Selected references:
Guo et al. (2021) The pentatricopeptide repeat protein GEND1 is required for root development and high temperature tolerance in Arabidopsis thaliana,Biochemical and Biophysical Research Communications,Volume 578,2021,Pages 63-69,ISSN 0006-291X,https://doi.org/10.1016/j.bbrc.2021.09.022.(https://www.sciencedirect.com/science/article/pii/S0006291X21013164)Wang et al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 .Dai et al. (2020). Pentatricopeptide repeat protein DEK46 is required for multi-sites mitochondrial RNA editing and maize seed development. J Exp Bot. 2020 Jul 25;eraa348.doi: 10.1093/jxb/eraa348. Waltz et al. (2019). Small is big in Arabidopsis mitochondrial ribosome. Nat Plants. 2019 Jan;5(1):106-117. doi: 10.1038/s41477-018-0339-y.Doronina et al. (2019). Structural and Functional Features of the Wheat Embryo Sac’s Antipodal Cells during Differentiation. Russ J Dev Biol 50, 194–208. (immunolocalization)
ClpB protein is essential for resistance to high temperature stress. It functions to dissolve inactive protein aggregates that accumulate at high temperatures. Giese and Vierling (2002) Changes in oligomerization are essential for the chaperone activity of a small heat shock protein in vivo and in vitro. J Biol Chem; 277(48): 46310-8.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Synechocystis PCC 6803, Solanum lycopersicum
Expected Species:
Cyanobacteria, Francisella sp. Species of your interest not listed? Contact us
Immunogen:
recombinant clpB1 protein, derived from Synechocystis PCC 6803 strain slr1641 sequence; protein has an internal translation site. The nomenclature used is reverse of what is mentioned in the cyanobase.
OEP 75 or Toc75; Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded -barrel (EMBO J. (1995) 14:11, 2436-2446); In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87; Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane; YaeT; outer membrane protein assembly complex, (TIGR03303); FhaC; Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Pisum sativum, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus
Expected Species:
Oryza sativa, Ricinus communis, Populus trichocarpa, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
psTOC75; Predicted POTRA Domain #1; Amino acids, 158-241; Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. Q43715
Applications:
Flow cytometry (Flow cyt), Immunolocalization (IL),Western blot (WB)
HSP90-1 (heast shock protein 90-1) is an isoform involved in response to bacterium, arsenic and heat. Synonymes: ATHS83; ATHSP90.1; F6N7.13; F6N7_13; HEAT SHOCK PROTEIN 81-1; HEAT SHOCK PROTEIN 83; HEAT SHOCK PROTEIN 90.1; HSP81-1; HSP81.1; HSP83.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Szadeczky-Kardoss et al. (2022) Elongation factor TFIIS is essential for heat stress adaptation in plants. Nucleic Acids Res. 2022 Feb 28;50(4):1927-1950. doi: 10.1093/nar/gkac020. PMID: 35100405; PMCID: PMC8886746.Bychkov et al. (2022) The role of PAP4/FSD3 and PAP9/FSD2 in heat stress responses of chloroplast genes. Plant Sci. 2022 Sep;322:111359. doi: 10.1016/j.plantsci.2022.111359. Epub 2022 Jun 20. PMID: 35738478.Cvetkovska et al. (2022) A constitutive stress response is a result of low temperature growth in the Antarctic green alga Chlamydomonas sp. UWO241. Plant, Cell & Environment, 45, 156– 177. https://doi.org/10.1111/pce.14203Mishra et al. (2021) Interplay between abiotic (drought) and biotic (virus) stresses in tomato plants. Mol Plant Pathol. 2021 Dec 30. doi: 10.1111/mpp.13172. Epub ahead of print. PMID: 34970822.Shteinberg et al. (2021) Tomato Yellow Leaf Curl Virus (TYLCV) Promotes Plant Tolerance to Drought. Cells. 2021 Oct 25;10(11):2875. doi: 10.3390/cells10112875. PMID: 34831098; PMCID: PMC8616339.
Special application note:
Antibody is recognizing both, heat inducible Hsp90-1 and constitutive isofrom Hsp90-2. Both proteins have ca. 85 % similarity.This product can be sold containing ProClin if requested
Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Medicago truncatula, Oryza sativa, Phaseolus vulgaris, Pisum sativum, Populus trichocarpa, Spinacia oleracea, Solanum tuberosum, Triticum aestivum, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide conserved in higher plant mitochondrial HSC70 including Arabidopsis thaliana mtHSC70-1 Q8GUM2, At4g37910 and mtHSC70-2 Q9LDZ0, At5g09590
For reconstitution add 50 l of sterile water per tube
Molecular Weight:
73 | 70 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Lentini et al. (2018). Early responses to cadmium exposure in barley plants: effects on biometric and physiological parameters. Acta Physiol Plant (2018) 40: 178. https://doi.org/10.1007/s11738-018-2752-2.Rurek et al. (2018). Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought Involves Impaired Coordination of Transcriptomic and Proteomic Response and Regulation of Various Multifunctional Proteins. Preprints 2018, 2018010276 (doi: 10.20944/preprints201801.0276.v1).Opalińska et al. (2017). Identification of Physiological Substrates and Binding Partners of the Plant Mitochondrial Protease FTSH4 by the Trapping Approach. Int J Mol Sci. 2017 Nov 18;18(11). pii: E2455. doi: 10.3390/ijms18112455.Murcha et al. (2016). Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria. Plant Physiol. 2016 Oct 27. pii: pp.01519.2016.
Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Lee et al (2021). Chaperone-like protein DAY plays critical roles in photomorphogenesis. Nat Commun. 2021 Jul 7;12(1):4194. doi: 10.1038/s41467-021-24446-5. PMID: 34234144; PMCID: PMC8263706.Jeran et al. (2021) The PUB4 E3 Ubiquitin Ligase Is Responsible for the Variegated Phenotype Observed upon Alteration of Chloroplast Protein Homeostasis in Arabidopsis Cotyledons. Genes (Basel). 2021 Sep 6;12(9):1387. doi: 10.3390/genes12091387. PMID: 34573369; PMCID: PMC8464772.Dogra et al. (2019). Impaired PSII proteostasis triggers an UPR-like response in the var2 mutant of Arabidopsis thaliana. J Exp Bot. 2019 Apr 16. pii: erz151. doi: 10.1093/jxb/erz151.Chen et al. (2018). TIC236 links the outer and inner membrane translocons of the chloroplast. Nature. 2018 Dec;564(7734):125-129. doi: 10.1038/s41586-018-0713-y.Lentini et al. (2018). Early responses to cadmium exposure in barley plants: effects on biometric and physiological parameters. Acta Physiol Plant (2018) 40: 178. https://doi.org/10.1007/s11738-018-2752-2.
SUMO3 (Small ubiquitin-related modifier 3) is a small polypeptide that becomes covalently attached to various intracellular proteins leading to their post-translational modification.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from SUMO3 sequence of Arabidopsis thaliana, UniProt: Q9FLP5, TAIR: AT5G55170
The antibody is recognizing recombinant SUMO3 and shows no cross reactivity to SUMO1/2
Application Details:
1 : 5000 for detection of recombinant SUMO3 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Saleh et al. (2015). Posttranslational Modifications of the Master Transcriptional Regulator NPR1 Enable Dynamic but Tight Control of Plant Immune Responses. Cell Host Microbe. 2015 Aug 12;18(2):169-82. doi: 10.1016/j.chom.2015.07.005.
DnaK2 belongs to the family of heat shock proteins 70 and functions as a chaperone involved in stress response. Dnak2 is heat induced but also present at high level under normal growth conditions.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Synechocystis PCC 6803
Expected Species:
Anabaena sp., Crocosphaera watsonii, Cyanothece sp. PCC 7424, Lyngbya sp. PCC 8106, Microcoleus chthonoplastes PCC 7420, Nodularia spumigena CCY 9414, Nostoc sp., Synechococcus sp. PCC 7942 and other cyanobacterial strains Species of your interest not listed? Contact us
Immunogen:
full length recombinant DnaK2 protein derived from Synechocystis PCC 6803 DnaK2 protein sequence UniProt: P22358
OEP 75 or Toc75; Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded -barrel (EMBO J. (1995) 14:11, 2436-2446); In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87; Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane; YaeT; outer membrane protein assembly complex, (TIGR03303); FhaC; Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum, Physcomitrium patens, Pisum sativum, Spinacia oleracea, Thellungiella salsuginea, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus sp.
Expected Species:
Aegilops tauschii, Ananas comosus, Anthurium amnicola, Capsicum annuum, Catalpa bungei, Cicer arietinum, Cucumis melo, Glycine soja, Gossypium arboreum, Medicago truncatula, Morus notabilis, Nelumbo nucifera, Nicotiana sylvestris, Noccaea caerulescens, Populus trichocarpa, Ricinus communis, Sorghum bicolor, Theobroma cacao, Zostera marina, Vigna radiata, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
Pisum sativum TOC75; Predicted POTRA Domain #3; Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. UniProt: Q43715
Applications:
Flow Cytometry (Flow cyt), Immunolocalization (IL), Western blot (WB)
88 | 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Sasaki and Yamamoto (2015). Arabidopsis LAZY1 is a peripheral membrane protein of which the carboxy-terminal fragment potentially interacts with microtubules. Copyright 2015 The Japanese Society for Plant Cell and Molecular Biology, Plant Biotechnology, 32, 1–6 (2015) DOI: 10.5511/plantbiotechnology.15.0116aVera-Estrella et al. (2014). Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea. J Proteomics. 2014 Jun 2. pii: S1874-3919(14)00288-7. doi: 10.1016/j.jprot.2014.05.018.Hsueh et al. (2014). The chloroplast outer envelope protein P39 in Arabidopsis thaliana belongs to the Omp85 protein family. Proteins. 2014 Nov 17. doi: 10.1002/prot.24725.
ClpB2 is essential for organism and can not be complemented by a mutation in ClpB1 gene. This cytoplasmic protein is not involved in thermotolerance. Giese and Vierling (2002) Changes in oligomerization are essential for the chaperone activity of a small heat shock protein in vivo and in vitro. J Biol Chem; 277(48): 46310-8.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Synechocystis PCC 6803
Expected Species:
Cyanobacteria Species of your interest not listed? Contact us
Immunogen:
recombinant slr0156 protein, derived from Synechocystis PCC 6803 strain slr0156 sequence. This protein is annotated as ClpB1 in a data base but was originally named ClpB2 according to the paper of Giese and Vierling (2002).
Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein P05067
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Human
Expected Species:
Bovine, Chicken, Dog, Porcine, Rabbit
Immunogen:
synthetic peptide chosen from human Abeta (1-42) protein, Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V-I-A
Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Human
Expected Species:
Primates, Chimpanzee, Gorilla
Immunogen:
recombinant, full length, human alpha-synuclein UniProt: P37840, epitope was mapped between amino acid 1-15
Applications:
ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
Bargar et al. (2021) Discrimination of MSA-P and MSA-C by RT-QuIC analysis of olfactory mucosa: the first assessment of assay reproducibility between two specialized laboratories. Mol Neurodegener. 2021 Dec 11;16(1):82. doi: 10.1186/s13024-021-00491-y. PMID: 34895275; PMCID: PMC8665327.Br nnstr m et al. (2014). A generic method for design of oligomer-specific antibodies. PLoS One. 2014 Mar 11;9(3):e90857. doi: 10.1371/journal.pone.0090857. eCollection 2014.
Special application note:
Immunolocalization: human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95 C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit.This antibody will recognize human SNCA monomers and multimers in Western blot and can be used to detect fibrills in a sandwich ELISA. In ELISA this antibody can be used for detection, combined with AS13 2719 as a capture antibody.
Amylin, or Islet Amyloid Polypeptide (IAPP) P10997, is a 37-residue peptide hormone secreted by pancreatic beta-cells at the same time as insulin (in a roughly 1:100 amylin:insulin ratio). Islet, or insulinoma, almyloid polypeptide (IAPP, or amylin) is commonly found in pancreatic islets of patients suffering diabetes mellitus type 2, or harboring an insulinoma. While the association of amylin with the development of type 2 diabetes has been known for some time, a direct causative role for amylin has been harder to establish. Recent results suggest that amylin, like the related beta-amyloid (Abeta) associated with Alzherimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the deleopment of type 2 diabetes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Human
Expected Species:
Primates, mouse, rat, dog, seal, Chinese hamster
Immunogen:
Synthetic peptide corresponding to the human the 37 residue IAPP also known as amylin, The IAPP/amylin peptide contains a disulphide-bridge between Cys2-Cys7 Amino acid sequence: KCNTATCATQRLANFLVHSSNNFGAILSSTNVGSNTY (disulphide link between Cys2-Cys7)
Gene Transfer Agents (GTAs) are bacteriophage-like particles which function as a means to package and transfer genomic DNA between bacterial cells.GTA-mediated gene transfer might be an important phenomenon in natural microbial communities.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Rhodobacterales Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated conserved peptide sequence found in the Gene Transfer Agent (GTA) major capsid protein (MCP) encoded in Bacteria within the Rhodobacterales order of the class alpha-Proteobacteria including Rhodobacter sphaeroides UniProt: Q3J3K4
For reconstitution add 200 l of sterile distilled water
Molecular Weight:
31.4 | 32 kDa (predicted mature capsid protein of Rhodobacter capsulatus)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Shimizu et al. (2022) Persulfide-Responsive Transcription Factor SqrR Regulates Gene Transfer and Biofilm Formation via the Metabolic Modulation of Cyclic di-GMP in Rhodobacter capsulatus, Microorganisms 10, no. 5: 908. https://doi.org/10.3390/microorganisms10050908Koppenhofer et al. (2019). Integrated Transcriptional Regulatory Network of Quorum Sensing, Replication Control, and SOS Response in Dinoroseobacter shibae. Front. Microbiol., 12 April 2019 | https://doi.org/10.3389/fmicb.2019.00803.Tomasch et al. (2018). Packaging of Dinoroseobacter shibae DNA into Gene Transfer Agent Particles Is Not Random. Genome Biol Evol. 2018 Jan 1;10(1):359-369. doi: 10.1093/gbe/evy005.Mercer and Lang (2014). Identification of a predicted partner-switching system that affects production of the gene transfer agent RcGTA and stationary phase viability in Rhodobacter capsulatus. BMC Microbiol. 2014 Mar 19;14(1):71.
Special application note:
This product can be sold containing ProClin if requested
APX plays a key role in plant antioxidant system by reducing hydrogen peroxide to water. Cellular localization includes chloroplast (tAPX and sAPX), cytosol (cAPX) and peroxisome (pAPX).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Brassica rapa subsp. oleifera Stromal APX; Glycine max, Glycine soja L-ascorbate peroxidase T, chloroplastic; Medicago truncatula thylakoid-bound APX; Mesembryanthemum crystallinum, Pisum sativum Chloroplast stromal ascorbate peroxidase 12; Solanum lycopersicum thylakoid-bound APX; Spinacia oleracea stromal APX; Theobroma cacao L-APX T isoform 3; Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
BSA-conjugated synthetic peptide derived from Arabidopsis thaliana tAPX (thylakoidal ascorbate peroxidase) UniProt: Q42593-1, TAIR:At1g77490 and sAPX (stromal/mitochondrial ascorbate peroxidase) UniProt: Q42592-1 TAIR: At4g08390 Five out of twelve amino acids are also identical with cAPX1 (At1g07890), cAPX2 (At3g09640) and pAPX (At4g35000)
This product can be sold containing proclin if requested
Application Details:
1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
25-38 kDa for A. thaliana
Not reactive in:
Algae, Helianthus annus, Marchantia polymorpha
Selected references:
Wang et al. (2022) Reciprocity between a retrograde signal and a putative metalloprotease reconfigures plastidial metabolic and structural states. Sci Adv. 2022 Jun 3;8(22):eabo0724. doi: 10.1126/sciadv.abo0724. Epub 2022 Jun 3. PMID: 35658042; PMCID: PMC9166295.Kucko et al. (2022) The acceleration of yellow lupine flower abscission by jasmonates is accompanied by lipid-related events in abscission zone cells, Plant Science, Volume 316, 2022,111173, ISSN 0168-9452, https://doi.org/10.1016/j.plantsci.2021.111173. (https://www.sciencedirect.com/science/article/pii/S0168945221003691)Jedelska et al. (2021) Protein S-nitrosation differentially modulates tomato responses to infection by hemi-biotrophic oomycetes of Phytophthora spp. Hortic Res. 2021 Feb 1;8(1):34. doi: 10.1038/s41438-021-00469-3. PMID: 33518717; PMCID: PMC7848004.Tokarz et al. (2021). Stem Photosynthesis-A Key Element of Grass Pea (Lathyrus sativus L.) Acclimatisation to Salinity. Int J Mol Sci. 2021 Jan 12;22(2):685. doi: 10.3390/ijms22020685. PMID: 33445673; PMCID: PMC7828162.Tokarz et al. (2020). Can Ceylon Leadwort ( Plumbago zeylanica L.) Acclimate to Lead Toxicity?-Studies of Photosynthetic Apparatus Efficiency. Int J Mol Sci. 2020 Mar 9;21(5):1866.doi: 10.3390/ijms21051866.
UPF0603 protein At1g54780, chloroplastic is located in thylakoid lumen. The protein seem to be required for high light tolerance of PSII. Sirpi et al. (2007) TLP18.3, a novel thylakoid lumen protein regulating photosystem II repair cycle. Biochem J; 406(3): 415-425
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Oryza sativa, Ricinus communis, Zea mays Species of your interest not listed? Contact us
Immunogen:
synthetic peptides amino acids 227-241 and amino acids 242-256 from Arabidopsis thaliana Tlp18.3 protein sequence TAIR: At1g54780)
Application note: in a gel without urea Tlp18,3 will run at 18 kDa, while in a gel with urea it runs at 25 kDa
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water
Molecular Weight:
22 | 18.5 kDa (A. thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Zienkiewicz et al. (2013).Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plan Physiol Biochem. March 16.
Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide conserved in known higher plant HSC70 proteins including three isoforms of Arabidopsis thaliana HSC70-1 UniProt: F4KCE5 , HSC70-2 UniProt: A0A178UTH3 and HSC70-3 Uniprot: O65719 as well as heat shock inducible Hsp70 of Arabidopsis thaliana TAIR: AT3g12580/T2E22_110 and At1g16030 and AT3g12580/T2E22_110
Can be sold containing 0.1% ProClin if requested This antibody can be used as a marker of cytoplasmic fraction in tomato (Anfoka et al. 2015).Applied primary antibody dilution in western blot depends upon sensitivity of detection reagents (pico or femtogram for chemiluminescent detection).Immunoprecipitation protocol using Agrisera anti-Hsp70 cytosolic antibodies, see tab: protocols.
Application Details:
1 : 3000 - 1: 10 000, 5 g protein/well (WB), 2-3 l/protein extract of concentration 3-5 mg/ml
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
70 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Chong et al. (2022) The tomato OST1-VOZ1 module regulates drought-mediated flowering. Plant Cell. 2022 Apr 26;34(5):2001-2018. doi: 10.1093/plcell/koac026. PMID: 35099557; PMCID: PMC9048945.Bychkov et al. (2022) The role of PAP4/FSD3 and PAP9/FSD2 in heat stress responses of chloroplast genes. Plant Sci. 2022 Sep;322:111359. doi: 10.1016/j.plantsci.2022.111359. Epub 2022 Jun 20. PMID: 35738478.Cvetkovska et al. (2022) A constitutive stress response is a result of low temperature growth in the Antarctic green alga Chlamydomonas sp. UWO241. Plant, Cell & Environment, 45, 156– 177. https://doi.org/10.1111/pce.14203Wang et al. (2022) 17-(Allylamino)-17-demethoxygeldanamycin treatment induces the accumulation of heat shock proteins and alleviates senescence in broccoli. Postharvest Biology and Technology,Volume 186, 2022, 111818, ISSN 0925-5214, https://doi.org/10.1016/j.postharvbio.2021.111818.Kumari et al. (2021) In-depth assembly of organ and development dissected Picrorhiza kurroa proteome map using mass spectrometry. BMC Plant Biol. 2021 Dec 22;21(1):604. doi: 10.1186/s12870-021-03394-8. PMID: 34937558; PMCID: PMC8693493.
Special application note:
This product can be sold containing ProClin if requested
Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.Source of HSP70 standard: recombinant Hsp70 of Arabidopsis thaliana UniProt: Q9LHA8, TAIR: AT3G12580
Product Type:
Antibody
Format:
Lyophilized in glycerol
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Concentration: after re-constitution with sterile milliQ water final concentration of the standard is 0.15 pmoles/ lProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:
Standard curve: 3 loads are recommended (eg 0.1, 0.2, 0.3 pmol). Adjust range to fit your samples and your experiment. For most applications a sample load of 0.2 μg of chlorophyll/well will give a HSP70 signal in this range.Positive control: a 2 μl load per well is optimal for most chemiluminescent detection systems. Higher standard concentration needs to be used to allow detection by Coomasie stains. Such gels with higher standard concentration can not be used for quantitation using chemiluminescence.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently. Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:
For reconstitution add 82 l of steril water. Please notice that this product contains glycerol and might appear as liquid but is provided lyophilized. Final standard concentration is going to be 0.15 pmol/ l
Molecular Weight:
70 kDa
Special application note:
The HSP70 protein standard can be used in a combination with Agrisera global antibiodies (AS08 371) to quantitate HSP70 from a wide range of species. Global antibodies are raised against highly conserved amino acid sequence. Quantitative western blot: detailed method description, video tutorial
Pex14p has a protein transporter activity and is involved in protein targeting into peroxisome. Submitted protein name: Genomic DNA, chromosome 5, P1 clone:MQB2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Cucumis melo, Magnaporthe oryzaeSpecies of your interest not listed? Contact us
Immunogen:
Recombinant, soluble N-terminal domain of Arabidopsis thaliana Pex14p UniProt:Q9FE40, TAIR: At5g62810) that mediates PEX5 and PEX19 binding. The transmembrane and coiled-coil domains of PEX14 were replaced with a dual StrepII-His6 tag.
Antibodies will detect Pex14 protein in both light- and dark-grown seedlings grown on petri dishes and in rosette leaves of adult plants grown in soil
Application Details:
1 : 10 000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 25 l of sterile water
Molecular Weight:
55,5 | 75-65 kDa (depending on the gel system)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Llinas et al. (2022) An Arabidopsis pre-RNA processing8a (prp8a) missense allele restores splicing of a subset of mis-spliced mRNAs. Plant Physiol. 2022 Aug 1;189(4):2175-2192. doi: 10.1093/plphys/kiac221. PMID: 35608297.Calero-Munoz et al. (2019). Cadmium induces reactive oxygen species-dependent pexophagy in Arabidopsis leaves. Plant Cell Environ. 2019 Sep;42(9):2696-2714. doi: 10.1111/pce.13597.McLoughlin et al. (2018). Maize multi-omics reveal roles for autophagic recycling in proteome remodelling and lipid turnover. Nat Plants. 2018 Dec;4(12):1056-1070. doi: 10.1038/s41477-018-0299-2.Gonzalez et al. (2018). A pex1 missense mutation improves peroxisome function in a subset of Arabidopsis pex6 mutants without restoring PEX5 recycling. Proc Natl Acad Sci U S A. 2018 Apr 3;115(14):E3163-E3172. doi: 10.1073/pnas.1721279115.Vincent et al. (2017). A genome-scale analysis of mRNAs targeting to plant mitochondria: upstream AUGs in 5' untranslated regions reduce mitochondrial association. Plant J. 2017 Dec;92(6):1132-1142. doi: 10.1111/tpj.13749.
Catalase peroxidase (EC 1.11.1.7) is a bifunctional antioxidant enzyme encoded by the katG-gene. It exhibits both, catalase and peroxidase activity which provides a protection against oxidative stress by neutralising hydrogen peroxide. The enzyme is present in a number of bacterial taxa, including cyanobacteria.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Synechocystis sp. PCC 6803
Expected Species:
Synechococcus sp. including PCC 7302 and S.elongatus (PCC 6301, PCC 7942); other bacteria Species of your interest not listed? Contact us
Immunogen:
Recombinant full length KatG protein from Synechocystis sp. PCC 6803 (accessions P73911 and sll1987) with six His-tag on the terminus.
This product can be sold containing ProClin if requested
Application Details:
1 : 3000, 10 g protein/lane (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
84.4 | 84.4 kDa for Synechocystis sp. PCC 6803
Selected references:
Hakkila et al. (2018). Group 2 Sigma Factors Are Central Regulators of Oxidative Stress Acclimation in Cyanobacteria. Plant Cell Physiol. 2018 Nov 8. doi: 10.1093/pcp/pcy221.Wenk et al. (2012). A universally conserved GTPase regulates the oxidative stress response in Escherichia coli. J Bio. Chem. Nov 8.
PBG is an enzyme involved in tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Thermosynechococcus elongatus
Expected Species:
Cyanobacteria Species of your interest not listed? Contact us
Glutamine synthetase (GlnA) is the key enzyme in the incorporation of mineral nitrogen into glutamine.This product is a recombinant GlnA protein standard, source Synechocystis strain PCC 6803.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Concentration: after adding 225 l of milliQ water final concentration of the standard is 0.20 pmol/ lProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:
Standard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2μg of chlorophyll will give a GlnA signal in this range.Positive control: a 2μl load per well is optimal for most chemiluminescent detection systems. This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:
For reconstitution add 225 l of sterile water
Molecular Weight:
in most gel systems GlnA migrates around 53 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
The GlnA protein standard can be used in combination with global anti-GlnA antibodies to quantitate GlnA from a wide range of species. Global antibodies are raised against highly conserved amino acid sequences in the GlnA protein.Quantitative western blot: detailed method description, video tutorial
D2 protein (PsbD) forms the reaction core of PSII (Photosystem II) as a heterodimer with the D1 protein (PsbA). PsbD is homologous to the D1 protein, with slightly higher molecular mass of about 39,5 kDa. Accumulation of D2 protein is an important step in the assemply of the PSII reaction centre complex.This product is a recombinant protein standard, source Synechocystis strain PCC 6803.
Product Type:
Antibody
Format:
Lyophilized in glycerol
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Concentration: after adding 225 l of milliQ water final concentration of the standard is 0.25 pmoles/ulProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:
Standard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2 μg of chlorophyll will give a PsbD signal in this range.Positive control: a 2 μl load per well is optimal for most chemiluminescent detection systems. This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:
For reconstitution add 225 l of sterile water, Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized
Molecular Weight:
In most gel systems PsbD migrates around 28-30 kDa
Selected references:
Partensky et al. (2018). Comparison of photosynthetic performances of marine picocyanobacteria with different configurations of the oxygen-evolving complex. Photosynth Res. 2018 Jun 25. doi: 10.1007/s11120-018-0539-3.Li et al. (2016). A Hard Day's Night: Diatoms Continue Recycling Photosystem II in the Dark. Front. Mar. Sci., 08 November 2016Li et al. (2014). The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytol. 2014 Sep 25. doi: 10.1111/nph.13037.
Special application note:
The PsbD protein standard can be used in combination with global anti-PsbD antibodies to quantitate PsbD from a wide range of species. Global antibodies are raised against highly conserved amino acid sequences in the PsbD protein.Quantitative western blot: detailed method description, video tutorial
Beta amylase (EC 3.2.1.2.) catalyzes the hydrolysis of the second alfa-1,4 glycosidic bond. Alternative names 1,4-alfa-D-glucan maltohydrolase, glycogenase,saccharogen. amylase).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Kappaphycus alvarezii, Solanum tuberosum
Expected Species:
Arabidopsis thaliana, Glycine max, Physcomitrium patens, Populus trichocarpa, Ricinus communis, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
Beta amylase isolated and purified from sweet potato UniProt:Q94EU9
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Application Details:
1 : 1000-1 : 4000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG in PBS labelled with biotin.
Reconstitution:
For reconstitution add 1 ml of sterile water
Molecular Weight:
60 kDa
Selected references:
Usuldin et al. (2017). Molecular investigation of carrageenan production in Kappaphycus alvarezii in different culture conditions: a proteomic approach. ournal of Applied Phycology, August 2017, Volume 29, Issue 4, pp 1989–2001. (Kappaphycus alvarezii)
Special application note:
Biotin/IgG protein molar ratio (B/P) is approximately 6,6, No foreign proteins are added, Marker used for lebling is N-hydroxysuccinimidoBiotin
Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4 C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation, It will not affect the performance of the product.
Host Animal:
Rabbit
Species Reactivity:
Cucurbita sp.
Expected Species:
Glycne max, Oryza sativa Species of your interest not listed? Contact us
IgG concentration is 10 mg/ml, Biotin/IgG protein molar ratio is approximately 5,6, No foreign proteins are added
Application Details:
1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)
Purity:
Purified IgG in PBS pH 7.2.
Reconstitution:
For reconstitution add 1 ml of sterile water, Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution, If necessary, centrifuge to remove any particulates, Prepare fresh working dilution daily
Molecular Weight:
61 | 45 kDa (Cucurbita pepo)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography, N-Hydroxysuccinimidobiotin is used for labelling of antibody
Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4 C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation, It will not affect the performance of the product.
Host Animal:
Rabbit
Species Reactivity:
Cucurbita sp.
Expected Species:
Glycne max, Oryza sativa Species of your interest not listed? Contact us
Immunogen:
ascorbate oxidase purified from Cucurbita sp.
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Lhcb5 is one of the 3 minor, highly conserved chlorophyll a/b-binding proteins associated with Photosystem II in plants and algae. As a part of the inner light-harvesting antenna it has been sugested to regulate (together with Lhcb4 and Lhcb6) the energy flow from the outer LHCII antenna to the PSII reaction center.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Chlamydomonas reinhardtii
Immunogen:
KLH-conjugated synthetic peptide derived from Chlamydomonas reinhardtii Lhcb5 protein sequence, UniProt:Q9FEK6
For optimal detection, load/well should be from 5 to 10 g of total protein. Higher protein load combined with weak blocking will contribute to detection of other bands.
Application Details:
1 : 5000-1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
30 | 26 kDa
Not reactive in:
Other algae
Selected references:
Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.Gonzaga Heredia-Martinez et al. (2018). Chloroplast damage induced by the inhibition of fatty acid synthesis triggers autophagy in Chlamydomonas. Plant Physiol, Sept. 2018.Correa-Galvis et al. (2016). Photosystem II Subunit PsbS Is Involved in the Induction of LHCSR Protein-dependent Energy Dissipation in Chlamydomonas reinhardtii. J Biol Chem. 2016 Aug 12;291(33):17478-87. doi: 10.1074/jbc.M116.737312.Muranaka et al. (2015). TEF30 interacts with photosystem II monomers and is involved in the repair of photodamaged photosystem II in Chlamydomonas reinhardtii. Plant Physiol. 2015 Dec 7. pii: pp.01458.2015.Drop et. al (2014). Consequences of state transitions on the structural and functional organization of Photosystem I in the green alga Chlamydomonas reinhardtii. Plant J. 2014 Feb 8. doi: 10.1111/tpj.12459.
Lhcbm5 is one of minor LHCII that associates with PSI in state-2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Chlamydomonas reinhardtii
Immunogen:
SDS-PAGE purified polypeptide from Chlamydomonas reinhardtii LHCII-type II-enriched fractions
For western blot detection image please refer to the article below
Application Details:
1 : 5000-1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
29 | 30 kDa
Selected references:
Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.Pinnola (2021). The rise and fall of Light-Harvesting Complex Stress-Related proteins as photoprotection agents during evolution. J Exp Bot. 2019 Oct 24;70(20):5527-5535. doi: 10.1093/jxb/erz317. PMID: 31424076.Nama et al. (2018). Non-photochemical quenching-dependent acclimation and thylakoid organization of Chlamydomonas reinhardtii to high light stress. Photosynth Res. 2018 Jul 7. doi: 10.1007/s11120-018-0551-7.Jeong et al. (2017). Deletion of the chloroplast LTD protein impedes LHCI import and PSI-LHCI assembly in Chlamydomonas reinhardtii. J Exp Bot. 2017 Dec 30. doi: 10.1093/jxb/erx457.
Special application note:
This antibody cross-reacts with three major LHCII proteins of Chlamydomonas, which are slightly smaller than Lhcam5 on SDS-gel. 6M urea SDS-PAGE is one of the best systems that separate Lhcbm5 and the other major LHCII proteins. The dilution of the antibody should be carefully determined to reduce the cross-reactions with other major LHCII proteins, we recommend for this purpose to use the dilution of 1: 10 000- 1: 50 000This product can be sold containing ProClin in requested.
RbcL antibody and protein standard: Please store at -20 °C (6 months) or -80°Cfor long term storage(years). Please avoid freezing and thawing of reconstituted antibodies, make aliquots instead. PEB extraction buffer:Stable at RT for at least 1 month. For short-term storage please stoore (1 month) at 4°Cand for long term storage (1 year) at -20 °C.
Alpha proteobacteria, Algae (brown and red), Beta-proteobacteria, Dicots, Conifers, Cryptomonad, Cyanobacteria, Gamma-proeobacteria, Liverworts, Mosses, Prochlorophytes, PelwitschiaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide was used to elicit anti-RbcL antibody. No baculovirus was used in production of this product.
1 x 50 µl of AS03 037, RbcL | Rubisco large subunit, form I and form II (amount enough for 50-100 Western blots)1 x 100 µl of AS01 017S, Rubisco protein standard (0.15 pmoles / µl, amount enough for generation of standard curve in 34 assays (standard curve: 0.0625 pmoles, 0.125 pmoles, 0.25 pmoles)2 x 2 ml of AS08 300, Protein extraction buffer (amount enough for 48 isolations of plant material, using 500 µl 1x PEB for 100 mg fresh weight) or 120 isolations of algal material (using 200 µl 1x PEB for cell amounts corresponding to 4-10 µg total chlorophyll)2 x 10 µl of AS09 602, Goat anti-rabbit IgG (H&L), HRP conjugated(amount enough for 50-100 Western blots)1 X 10 ml of AS16 ECL-N-10, AgriseraECL Bright 10 ml (5 ml of component A + 5 ml of component B, trial pack)
Quantitative western blot: detailed method description, video tutorialDiscussion over some critical aspects of quantitative western blot: Heidebrecht et al. (2009). Improved semiquantitative Western blot technique with increased quantification range. J. Immunological Methods. 35:40-48.
Defez et al. (2019). Bacterial IAA-Delivery into Medicago Root Nodules Triggers a Balanced Stimulation of C and N Metabolism Leading to a Biomass Increase. Microorganisms. 2019 Sep 29;7(10). pii: E403. doi: 10.3390/microorganisms7100403.Sorrentino et al. (2018). Performance of three cardoon cultivars in an industrial heavy metal-contaminated soil: Effects on morphology, cytology and photosynthesis. J Hazard Mater. 2018 Jun 5;351:131-137. doi: 10.1016/j.jhazmat.2018.02.044.Mota et al. (2015). Effects of heavy metals on Cyanothece sp. CCY 0110 growth, extracellular polymeric substances (EPS) production, ultrastructure and protein profiles. J Proteomics. 2015 Apr 29;120:75-94. doi: 10.1016/j.jprot.2015.03.004.Thamatrakoln et al. (2013). Death-specific protein in a marine diatom regulates photosynthetic responses to iron and light availability. Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20123-8. doi: 10.1073/pnas.1304727110. Supplemental material describes western blot quantification method.
FtsZ1 (cell division protein FtsZ homolog 1) is required for plastid cell division. Localization: pollen grain and plastids of vegetative cells. Alternative names: Chloroplast FtsZ, Protein accumulation and replication of chloroplasts 10, Protein plastid movement impaired4.FtsZ2 (cell visision protein FtsZ homolog 2) is required for plastid cell division. Present in two isoforms: FtsZ2-1 and FtsZ2-2. Alternative names: Plastid division protein FTSZ2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Hordeum vulgare
Expected Species:
Chlamydomons reinhardtii, Cucumis sativus, Gentiana lutea, Glycine max, Gossypium arobretum, Jatropha manihot, Lilium longiflorum, Lupinus angustifolius, Manihot esculenta, Marchantia aquatica, Medicago truncatula, Morus notabilis, Nannochloropsis gaditana, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, populus trichocarpa, Ricinus communis, Solanum lycopersicum, Sorgum bicolor, Theobroma cacao, Triticum uRatum, Zea mays, Yellow gentian, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
Recombinant part of Arabidopsis thalina FtsZ conserved in FtsZ1 Q42545 At5g55280 and FtsZ2 including FtsZ2-1 O82533, At2g36250 and FtsZ2-2 Q9LXJ0, At3g52750 and in a wide range of FtsZ proteins from other plant species.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots. Synonym: 9-(?-D-Ribofuranosyl)-trans-zeatin, N6-(trans-4-Hydroxy-3-methyl-2-buten-1-yl)adenosine
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
2-10 l/15 ml; Specific information about dilution is going to be included on the vial
Purity:
Total IgG. Protein G purified in PBS.
Reconstitution:
For reconstitution add 70 l of sterile water
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Ferreira et al. (2019). Enzyme-mediated metabolism in nutritive tissues of galls induced by Ditylenchus gallaeformans (Nematoda: Anguinidae). Plant Biol (Stuttg). 2019 May 18. doi: 10.1111/plb.13009.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
N6-isopentenyladenosine
Expected Species:
N6-isopentenyladenosine
Immunogen:
BSA-conjugated, via ribose, N6-isopentenyladenosine
This antibody recognize a6A but not m6A or A, which iis caused by the geometrical similarity between isopentenyl and allyl (Shu et al., 2020).
Application Details:
2-10 l/15 ml; Specific information about dilution is going to be included on the vial
Purity:
Total IgG. Protein G purified in PBS.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Shu et al. (2022) m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution, STAR Protocols, Volume 3, Issue 1, 2022, 101096, ISSN 2666-1667, https://doi.org/10.1016/j.xpro.2021.101096.Alvarez et al. (2020). Hormonal and gene dynamics in de novo shoot meristem formation during adventitious caulogenesis in cotyledons of Pinus pinea. Plant Cell Rep. 2020 Jan 28. doi: 10.1007/s00299-020-02508-0.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots. Synonym: 9-(β-D-Ribofuranosyl)-trans-zeatin, N6-(trans-4-Hydroxy-3-methyl-2-buten-1-yl)adenosine
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Dihydrozeatin riboside
Expected Species:
Dihydrozeatin riboside
Immunogen:
BSA-conjugated, via ribose, dihydrozeatin riboside
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Ortho-topolin riboside
Expected Species:
Ortho-topolin riboside
Immunogen:
BSA-conjugated, via ribose, ortho-topolin riboside
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
IAA | indole-3-acetic acid (C1')
Expected Species:
IAA | indole-3-acetic acid (C1')
Immunogen:
BSA-conjugated, via C1 carboxyl group, indole-3-acetic acid (C1')
Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Freeze upon arrival and store at -20 °C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Antibody can be used for direct detection of free IAA (see reference below)Antibody is provided in 50% glycerol. For larger quantity (1 mg)- please inquire.Steedman's wax embedding technique is recommended to be used with this antibody. The most critical issue is to keep the temperature below 37 C during the whole embedding procedure. More information can be found in Vitha et al. (2000).
Application Details:
Specific information about dilution is going to be included on the vial
Purity:
Total IgG. Protein G purified in PBS.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Nukazuka et al. (2021). A Role for Auxin in Triggering Lamina Outgrowth of Unifacial Leaves. Plant Physiol. 2021 Feb 23:kiab087. doi: 10.1093/plphys/kiab087. Epub ahead of print. PMID: 33620494.Dinis et al. (2018). Kaolin modulates ABA and IAA dynamics and physiology of grapevine under Mediterranean summer stress. J Plant Physiol. 2018 Jan;220:181-192. doi: 10.1016/j.jplph.2017.11.007.Escand n et al. (2016). Integrated physiological and hormonal profile of heat-induced thermotolerance in Pinus radiata. Tree Physiol. 2016 Jan;36(1):63-77. doi: 10.1093/treephys/tpv127. Epub 2016 Jan 12.Jesus et al. (2015). Salicylic acid application modulates physiological and hormonal changes in Eucalyptus globulus under water deficit. Environ and Exp Botany, Volume 118, October 2015, Pages 56?66.De Diego et al. (2013).Immunolocalization ofIAA andABA inroots andneedles ofradiatapine (Pinusradiata) duringdrought andrewatering. Tree Physiol., May; 33(5):537-549.
Abscisic acid (ABA) is a plant hormone involved in different physiological responses as stimulation of the closure of stomata (water stress brings about an increase in ABA synthesis), iInhibition of shoot growth, and many others. ABA shown to have both inhibitory as well as many promoting functions.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Abscisic acid (C1)
Expected Species:
Abscisic acid (C1)
Immunogen:
BSA-conjugated abscisic acid (C1) via C1 carboxyl group
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots. Synonym: 9-(β-D-Ribofuranosyl)-trans-zeatin, N6-(trans-4-Hydroxy-3-methyl-2-buten-1-yl)adenosine
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots. Synonym: 9-(β-D-Ribofuranosyl)-trans-zeatin, N6-(trans-4-Hydroxy-3-methyl-2-buten-1-yl)adenosine
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For 1 mg of antibodies For ELISA and immunoassay or For ELISA kit - please inquire, Antibodies are supplied in 50% glycerol,This antibody cannot discriminate between the zeatin ribozide and zeatin, Zeatin cross-reactivity is usually lower ca,50%,BSA can be used For blocking using this antibody
Application Details:
Specific information about dilution is going to be included on the vial
Purity:
Total IgG. Protein G purified in PBS.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Han et al. (2019). Characterization and T-DNA insertion sites identification of a multiple-branches mutant br in Betula platyphylla and Betula pendula. BMC Plant Biol. 2019 Nov 12;19(1):491. doi: 10.1186/s12870-019-2098-y..
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
N6-isopentenyladenosine
Expected Species:
N6-isopentenyladenosine
Immunogen:
BSA-conjugated, via ribose, N6-isopentenyladenosine
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
N6-isopentenyladenosine
Expected Species:
N6-isopentenyladenosine
Immunogen:
BSA-conjugated, via ribose, N6-isopentenyladenosine
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Dihydrozeatin riboside
Expected Species:
Dihydrozeatin riboside
Immunogen:
BSA-conjugated, via ribose, dihydrozeatin riboside
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Dihydrozeatin riboside
Expected Species:
Dihydrozeatin riboside
Immunogen:
BSA-conjugated, via ribose, dihydrozeatin riboside
For 1 mg of antibodies For ELISA and immunoassay or For ELISA kit - please inquire, Antibodies are supplied in 50% glycerol
Application Details:
Specific information about dilution is going to be included on the vial
Purity:
Total IgG. Protein G purified in PBS.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Alvarez et al. (2020). Hormonal and gene dynamics in de novo shoot meristem formation during adventitious caulogenesis in cotyledons of Pinus pinea. Plant Cell Rep. 2020 Jan 28. doi: 10.1007/s00299-020-02508-0.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Specific information about dilution is going to be included on the vial
Purity:
Total IgG. Protein G purified in PBS.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Alvarez et al. (2020). Hormonal and gene dynamics in de novo shoot meristem formation during adventitious caulogenesis in cotyledons of Pinus pinea. Plant Cell Rep. 2020 Jan 28. doi: 10.1007/s00299-020-02508-0.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Ortho-topolin riboside
Expected Species:
Ortho-topolin riboside
Immunogen:
BSA-conjugated, via ribose, ortho-topolin riboside
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots. Synonym: 9-(?-D-Ribofuranosyl)-trans-zeatin, N6-(trans-4-Hydroxy-3-methyl-2-buten-1-yl)adenosine
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Bowie et al. (2018). N6-Furfuryladenine is protective in Huntington's disease models by signaling huntingtin phosphorylation. Proc Natl Acad Sci U S A. 2018 Jul 24;115(30):E7081-E7090. doi: 10.1073/pnas.1801772115.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Indole 3 acetic acid (IAA) is the principal auxin in higher plants. This hormone is produced in in cells in the apex and young leaves of a plant. Plant cells synthesize IAA from tryptophan. Different effects caused by auxines include: induction of cell elongation and cell division and have a subsequent results for plant growth and development
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
indole-3-acetic acid (C1')
Expected Species:
IAA | indole-3-acetic acid (C1')
Immunogen:
BSA- conjugated, via C1 carboxyl group, indole-3-acetic acid (C1')
For 1 mg of antibodies or For ELISA kit - please inquire
Application Details:
to be determined by end user
Purity:
Total IgG. Protein G purified in PBS.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Livanos et al. (2015). Deliberate ROS production and auxin synergistically trigger the asymmetrical division generating the subsidiary cells in Zea mays stomatal complexes. Protoplasma. 2015 Aug 7.
Abscisic acid (ABA) is a plant hormone involved in different physiological responses as stimulation of the closure of stomata (water stress brings about an increase in ABA synthesis), iInhibition of shoot growth, and many others. ABA shown to have both inhibitory as well as many promoting functions.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Abscisic acid (C1) in Arabidopsis thaliana, Eucalyptus globulus, Petunia hybrida L., Pinus radiata, Populus trichocarpa
Expected Species:
Abscisic acid (C1)
Immunogen:
BSA-conjugated abscisic acid (C1) via C1 carboxyl group
The antibody will recognize either the ABA conjugated to glucose ester (ABA-GE) or the ABA precursor: abscisic acid aldehyde, ABA aldehyde is however not usually present in plant tissue similarly to ABA alcohol which is also reactive, The antibodies will predominantly recognize only ABA and its glucosylester
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Total IgG. Protein G purified in PBS with 50 % glycerol.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Wojciechowska et al. (2020). Abscisic Acid and Jasmonate Metabolisms Are Jointly Regulated During Senescence in Roots and Leaves of Populus trichocarpa. Int J Mol Sci , 21 (6) Dinis et al. (2018). Kaolin modulates ABA and IAA dynamics and physiology of grapevine under Mediterranean summer stress. J Plant Physiol. 2018 Jan;220:181-192. doi: 10.1016/j.jplph.2017.11.007. Kovaleva et al. (2017). ABA and IAA control microsporogenesis in Petunia hybrida L. Protoplasma. 2017 Nov 13. doi: 10.1007/s00709-017-1185-x. Escand n et al. (2016). Integrated physiological and hormonal profile of heat-induced thermotolerance in Pinus radiata. Tree Physiol. 2016 Jan;36(1):63-77. doi: 10.1093/treephys/tpv127. Epub 2016 Jan 12.Ondzighi-Assoume et al. (2016). Environmental Nitrate Stimulates Root Tip Abscisic Acid Accumulation via Release from Inactive Stores. Plant Cell. 2016 Feb 17. pii: TPC2015-00946-RA.Jesus et al. (2015). Salicylic acid application modulates physiological and hormonal changes in Eucalyptus globulus under water deficit. Environ and Exp Botany, Volume 118, October 2015, Pages 56–66.Lacuesta et al. (2013). Immunolocalization of IAA and ABA in roots and needles of radiata pine (Pinus radiata) during drought and rewatering. Tree Physiol. May;33(5):537-49.
Cytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling. Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Ortho-topolin riboside
Expected Species:
Ortho-topolin riboside
Immunogen:
BSA-conjugated, via ribose, ortho-topolin riboside
Beta-glucosidase (EC=3.2.1.21) is an enzyme which catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of glucose acting upon upon β1->4 bonds linking two glucose or glucose-substituted molecules.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Prunus dulcis
Immunogen:
native beta- glucosidase purified from almonds
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography
Application Details:
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG in PBS.
Reconstitution:
For reconstitution add 1 ml of sterile destilled water
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Gong et al (2022) The promising application of a beta-glucosidase inhibitor in the postharvest management of Volvariella volvacea, Postharvest Biology and Technology,Volume 185,2022,111784,ISSN 0925-5214,https://doi.org/10.1016/j.postharvbio.2021.111784.
Special application note:
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Beta-glucosidase (EC=3.2.1.21) is an enzyme which catalyses the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of glucose acting upon β1->4 bonds linking two glucose or glucose-substituted molecules.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Prunus dulcis
Immunogen:
Native beta- glucosidase purified from almonds
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry, paraffin (IHC), Western blot (WB)
Biotin/IgG protein molar ration is approximately 6,2, No foreign proteins are added
Application Details:
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG in PBS pH 7.2.
Reconstitution:
For reconstitution add 1 ml of sterile destilled water
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is labelled with biotin using N-hydroxysuccinimidobiotin, Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Beta-glucosidase (EC=3.2.1.21) is an enzyme which catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of glucose acting upon upon β1->4 bonds linking two glucose or glucose-substituted molecules.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Prunus dulcis
Immunogen:
native beta- glucosidase purified from almonds
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran
Application Details:
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Purity:
Immunogen affinity purified serum in PBS.
Reconstitution:
For reconstitution add 0,5 ml of sterile destilled water
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Alpha-mannosidase cleaves the alpha form of mannose.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Canavalia ensiformis (common jack-bean)
Immunogen:
native alpha-mannosidase purified from jack beans
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography
Application Details:
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG in PBS.
Reconstitution:
For reconstitution add 1 ml of sterile destilled water
Not reactive in:
Arabidopsis thaliana
Special application note:
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Alpha-mannosidase cleaves the alpha form of mannose.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Canavalia ensiformis (common jack-bean)
Immunogen:
Native alpha-mannosidase purified from jack beans
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Biotin/IgG protein molar ration is approximately 1,4, No foreign proteins are added
Application Details:
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG in PBS.
Reconstitution:
For reconstitution add 0,5 ml of sterile destilled water
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is labelled with biotin using N-hydroxysuccinimidobiotin, Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.Alternative names: ATPase subunit II, ATP synthase F(0) sector subunit b'
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Chlamydomonas reinhardtii
Expected Species:
Algae, Oryza sativa, Sorghum bicolor, Zea mays, Volvox carteri Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated mix of synthetic peptide derived from Arabidopsis thaliana AtpG Q0WMW8, At4g32260 and Chlamydomonas reinhardtii ATP synthase subunit b' A8J785
PEPC (phosphoenolpyruvate carboxylase), EC=4.1.1.31, belongs to an enzyme family of carboxy-lyases that is catalyzing adding fo carbon dioxide to phosphoenolpyruvate (PEP) to form oxaloacetate. Alternative names: PEPCase 1, PEPCase 3, PEPC 1, PEPC 3
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Please do not re-use this primary antibody solution. In case of cyanobacterial samples there will be no signal in your second incubation.
KLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species including Arabidopsis thaliana Q9MAH0, At1g53310 (PEPC 1), Q84VW9, At3g14940 (PEPC 3). The peptide chosen to elicit this antibody is also perfectly conserved in bacterial type of this enzyme NP_177043.2 (PEPC 4). For Zea mays, the peptide is converved in PEP1 and PEP4 isoforms.
Antibody can be also used following 2D gel electrophoresis
Application Details:
1 : 500 (IL), 1: 1000 - : 10 000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
110 | 105 kDa
Not reactive in:
Methanothermobacter thermautotrophicus
Selected references:
Durall et al. (2021). Production of succinate by engineered strains of Synechocystis PCC 6803 overexpressing phosphoenolpyruvate carboxylase and a glyoxylate shunt. Microb Cell Fact. 2021 Feb 8;20(1):39. doi: 10.1186/s12934-021-01529-y. PMID: 33557832; PMCID: PMC7871529.Wang et al. (2021). Brassinosteroids inhibit miRNA-mediated translational repression by decreasing AGO1 on the endoplasmic reticulum. J Integr Plant Biol. 2021 May 21. doi: 10.1111/jipb.13139. Epub ahead of print. PMID: 34020507.Rakhmankulova et al. (2021) Possible Activation of ?3 Photosynthesis in ?4 Halophyte Kochia prostrata Exposed to an Elevated Concentration of ??2. Russ J Plant Physiol 68, 1107–1114 (2021). https://doi.org/10.1134/S1021443721060169Durall et al. (2020). Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803. Biotechnol Biofuels. 2020 Jan 28;13:16. doi: 10.1186/s13068-020-1653-y. Kramer et al. (2020). N6?methyladenosine and RNA secondary structure affect transcript stability and protein abundance during systemic salt stress in Arabidopsis. Plant Direct . 2020 Jul 24;4(7):e00239.doi: 10.1002/pld3.239.
PEPC (phosphoenolpyruvate carboxylase), EC=4.1.1.31, belongs to an enzyme family of carboxy-lyases that is catalyzing adding fo carbon dioxide to phosphoenolpyruvate (PEP) to form oxaloacetate. Alternative names: PEPCase 1, PEPCase 3, PEPC 1, PEPC 3
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
KLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species including Arabidopsis thaliana Q9MAH0, At1g53310 (PEPC 1), Q84VW9, At3g14940 (PEPC 3). The peptide chosen to elicit this antibody is also perfectly conserved in bacterial type of this enzyme NP_177043.2 (PEPC 4)
PEPC (phosphoenolpyruvate carboxylase), EC=4.1.1.31, belongs to an enzyme family of carboxy-lyases that is catalyzing adding fo carbon dioxide to phosphoenolpyruvate (PEP) to form oxaloacetate. Alternative names: PEPCase 1, PEPCase 3, PEPC 1, PEPC 3
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
KLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species including Arabidopsis thaliana Q9MAH0, At1g53310 (PEPC 1), Q84VW9, At3g14940 (PEPC 3). The peptide chosen to elicit this antibody is also perfectly conserved in bacterial type of this enzyme NP_177043.2 (PEPC 4)
PEPC (phosphoenolpyruvate carboxylase), EC=4,1,1,31, belongs to an enzyme family of carboxy-lyases that is catalyzing adding fo carbon dioxide to phosphoenolpyruvate (PEP) to form oxaloacetate, Alternative names: PEPCase 1, PEPCase 3, PEPC 1, PEPC 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
KLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species including Arabidopsis thaliana Q9MAH0, At1g53310 (PEPC 1), Q84VW9, At3g14940 (PEPC 3). The peptide chosen to elicit this antibody is also perfectly conserved in bacterial type of this enzyme NP_177043.2 (PEPC 4). For Zea mays, the peptide is converved in PEP1 and PEP4 isoforms.
PEPC (phosphoenolpyruvate carboxylase), EC=4,1,1,31, belongs to an enzyme family of carboxy-lyases that is catalyzing adding fo carbon dioxide to phosphoenolpyruvate (PEP) to form oxaloacetate, Alternative names: PEPCase 1, PEPCase 3, PEPC 1, PEPC 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
KLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species including Arabidopsis thaliana Q9MAH0, At1g53310 (PEPC 1), Q84VW9, At3g14940 (PEPC 3). The peptide chosen to elicit this antibody is also perfectly conserved in bacterial type of this enzyme NP_177043.2 (PEPC 4). For Zea mays, the peptide is converved in PEP1 and PEP4 isoforms.
PEPC (phosphoenolpyruvate carboxylase), EC=4,1,1,31, belongs to an enzyme family of carboxy-lyases that is catalyzing adding fo carbon dioxide to phosphoenolpyruvate (PEP) to form oxaloacetate, Alternative names: PEPCase 1, PEPCase 3, PEPC 1, PEPC 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
KLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species including Arabidopsis thaliana Q9MAH0, At1g53310 (PEPC 1), Q84VW9, At3g14940 (PEPC 3). The peptide chosen to elicit this antibody is also perfectly conserved in bacterial type of this enzyme NP_177043.2 (PEPC 4). For Zea mays, the peptide is converved in PEP1 and PEP4 isoforms.
PEPC (phosphoenolpyruvate carboxylase), EC=4.1.1.31, belongs to an enzyme family of carboxy-lyases that is catalyzing adding fo carbon dioxide to phosphoenolpyruvate (PEP) to form oxaloacetate. Alternative names: PEPCase 1, PEPCase 3, PEPC 1, PEPC 3
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
KLH-conjugated synthetic peptide well conserved PEPC1 and sequences from different plant species including Arabidopsis thaliana Q9MAH0, At1g53310 (PEPC 1), Q84VW9, At3g14940 (PEPC 3). The peptide chosen to elicit this antibody is also perfectly conserved in bacterial type of this enzyme NP_177043.2 (PEPC 4)
Phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) serves as an important control element in the regulation of photosynthetic carbon metabolism in C4 and CAM plants. This is the first enzyme of the pathway, and PEPC enzymes are encoded by a small multigenic family. Several isoforms of PEPC have been characterised in maize, sorghum and sugarcane. These isoforms are involved in several functions such as the initial fixation of atmospheric CO2 (= C4 PEPC) and anaplerotic functions associated with nitrogen assimilation or amino acid biosynthesis (Lepiniec et al. 1994).
Product Type:
Antibody
Format:
Lyophilized in glycerol.
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Concentration: after re-constitution with sterile milliQ water final concentration of the standard is 0.15 pmoles/ lProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.This standard is ready-to-load and does not require any additions or heating.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:
Standard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2 μg of chlorophyll/well will give a RbcL signal in this range.Positive control: a 2 μl load per well is optimal for most chemiluminescent detection systems. Higher standard concentration needs to be used to allow detection by Coomasie stains. Such gels with higher standard concentration can not be used for quantitation using chemiluminescence.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:
For reconstitution add 60 l of steril water, Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized
Molecular Weight:
110 | 105 kDa
Special application note:
The PEPC protein standard can be used in a combination with Agrisera global PEPC antibiody to quantitate PEPC from a wide range of species. Global antibodies are raised against highly conserved amino acid sequence. Quantitative western blot: detailed method description, video tutorial
ClpB-P is involved in chloroplast biogenesis. Synonymes: ALBINO AND PALE GREEN 6, APG6, AT5G15450, CASEIN LYTIC PROTEINASE B3, CASEIN LYTIC PROTEINASE B-P, CLPB3, CLPB-P, T20K14_60, T20K14.60
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Populus trichocarpa, Ricinus communis, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from ClpB-P of Arabidopsis thaliana Q9LF37, At5g15450
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Jeran et al. (2021) The PUB4 E3 Ubiquitin Ligase Is Responsible for the Variegated Phenotype Observed upon Alteration of Chloroplast Protein Homeostasis in Arabidopsis Cotyledons. Genes (Basel). 2021 Sep 6;12(9):1387. doi: 10.3390/genes12091387. PMID: 34573369; PMCID: PMC8464772.Tieu Ngoc et al. (2020). N4-methylcytidine ribosomal RNA methylation in chloroplasts is crucial for chloroplast function, development, and abscisic acid response in Arabidopsis. J Integr Plant Biol. 2020 Sep 2. doi: 10.1111/jipb.13009. Epub ahead of print. PMID: 32876986.Han et al. (2015). A nuclear-encoded chloroplast-targeted S1 RNA-binding domain protein affects chloroplast rRNA processing and is crucial for the normal growth of Arabidopsis thaliana. Plant J. 2015 Jul;83(2):277-89. doi: 10.1111/tpj.12889. Epub 2015 Jun 15.
Special application note:
There is a cross-reacting band at ca, 95 kDa - therefore longer electrophoresis time is adviced to obtain good separation
AKIN beta-1 (AKINB1) is a member of SnRK1/SNF1/AMPK family in plants. It is a regulatory subunit of the putative trimeric SNF1-related protein kinase (SnRK) comples which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana AKIN beta-1 Q84VQ1, At5g21170
Belda-Palaz n et al. (2020) A dual function of SnRK2 kinases in the regulation of SnRK1 and plant growth. Nat Plants. 2020 Nov;6(11):1345-1353. doi: 10.1038/s41477-020-00778-w. Epub 2020 Oct 19. PMID: 33077877.Crozet et al. (2016). SUMOylation represses SnRK1 signaling in Arabidopsis. Plant J. 2016 Jan;85(1):120-133. doi: 10.1111/tpj.13096.Emanuelle et al. (2015). SnRK1 from Arabidopsis thaliana is an atypical AMPK. Plant J. 2015 Mar 3. doi: 10.1111/tpj.12813.
PsaD (PSI-D) is a core subunit of photosystem I highly conserved in all photosynthetic organisms (including bacteria with Fe-S type reaction centers). In eukaryots its encoded by 1 to 2 nuclear gene(s) and imported as a precursor into the chloroplast. In the thylakoid membrane it associates with PsaA and PsaB on the stromal site of the PSI core forming the Fd-docking site. PsaD is also required for the stable assembly of PsaC.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Alge, Dicots, Catalpa bungei, Cucumis melo, Conifers, Cyanidioschyzon merolae, Bigelowiella natans, Nannochloropsis sp. , Phaeodactylum tricornutum, Phyla dulcis, Zosteria marinaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide 100% conserved in all known plant PsaD sequences including Arabidopsis thaliana PSI-D1 UniProt:Q9S7H1 , TAIR: At4g02770 and PSI-D2 UniProt: Q9SA56 , TAIR At1g03130 as well as Physcomitrella patens. The conservation in Chlamydomonas reinhardtii is high (14 of 16 aminoacids are identical).
Applications:
Clear-native PAGE (CN-PAGE), Immunoprecipitation (IP), Western blot (WB)
This antibody is a replacement for former product, anti-PsaD AS04 046 Contains 0.1% ProClin.
Application Details:
1: 10 000 (CN-PAGE), 1 : 1000 - 1: 5 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
17.9 | 20 (for Arabidopsis thaliana)
Not reactive in:
Synechococcus elongatus sp. PCC 7942
Selected references:
Ivanov et al. (2022) The decreased PG content of pgp1 inhibits PSI photochemistry and limits reaction center and light-harvesting polypeptide accumulation in response to cold acclimation. Planta 255, 36 (2022). https://doi.org/10.1007/s00425-022-03819-0Fukura et al. (2021) Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions. J Plant Physiol. 2021 Nov;266:153535. doi: 10.1016/j.jplph.2021.153535. Epub 2021 Sep 25. PMID: 34607178.Fattore et al. (2021). Acclimation of photosynthetic apparatus in the mesophilic red alga Dixoniella giordanoi. Physiol Plant. 2021 Nov;173(3):805-817. doi: 10.1111/ppl.13489. Epub 2021 Jul 5. PMID: 34171145; PMCID: PMC8596783.Chen et al. (2021)Degradation of the photosystem II core complex is independent of chlorophyll degradation mediated by Stay-Green Mg2+ dechelatase in Arabidopsis,Plant Science,Volume 307,2021,110902,ISSN 0168-9452,https://doi.org/10.1016/j.plantsci.2021.110902. Pipitone et al. (2021). A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis. Elife. 2021 Feb 25;10:e62709. doi: 10.7554/eLife.62709. PMID: 33629953; PMCID: PMC7906606.
Special application note:
PsaD has frequently been used as a marker for intact PSI reaction centers.This product can be sold containing proclin if requested.
AKIN beta-2 (AKINB2) is a member of SnRK1/SNF1/AMPK family in plants. It is a regulatory subunit of the putative trimeric SNF1-related protein kinase (SnRK) comples which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana AKIN beta-2 Q9SCY5
V-ATPase c subunit is located in vacuole and is involved in ATP synthesis cuopled proton transport. This protein is coded by ATVHA-C3 gene. Alternative names: AT4g34720/T4L20_300
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Protein or membrane sample should be treated at 70 C for 10 min before loading on the gel
Application Details:
1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
16 | 16 kDa (Arabidopsis thaliana)
Not reactive in:
Algae
Selected references:
Vera-Estrella et al. (2017). Cadmium and zinc activate adaptive mechanisms in Nicotiana tabacum similar to those observed in metal tolerant plants. Planta. 2017 Apr 28. doi: 10.1007/s00425-017-2700-1.Barkla et al. (2016). Single-cell-type quantitative proteomic and ionomic analysis of epidermal bladder cells from the halophyte model plant Mesembryanthemum crystallinum to identify salt-responsive proteins. BMC Plant Biol. 2016 May 10;16(1):110. doi: 10.1186/s12870-016-0797-1.
Special application note:
Subunit c is one of most hydrophobic proteins (can be dissolved in organic solvent such as a mixture of chloroform/methanol solution). It is prone to aggregation even in the presence of SDS. Therefore, before loading on the gel membrane fractions should be incubated in buffer containing 2 % SDS at 60 or 70 C for 10 min or at 25 C for 30 min.
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Protein or membrane sample should be treated at 70 C for 10 min before loading on the gel, This antibody has so far not worked in IP
Application Details:
1 : 8000 (ELISA), 1 : 600 (IF), 1 : 2000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
73,5 | 80 kDa
Not reactive in:
Ostreococcus tauri
Selected references:
Gu et al. (2021) A Lipid Bodies-Associated Galactosyl Hydrolase Is Involved in Triacylglycerol Biosynthesis and Galactolipid Turnover in the Unicellular Green Alga Chlamydomonas reinhardtiiDittmer, Kleine, & Schwenkert. (2021) The TPR- and J-domain-containing proteins DJC31 and DJC62 are involved in abiotic stress responses in Arabidopsis thaliana. J Cell Sci. 2021 Oct 1;134(19):jcs259032. doi: 10.1242/jcs.259032. Epub 2021 Oct 12. PMID: 34515300.Shteinberg et al. (2021) Tomato Yellow Leaf Curl Virus (TYLCV) Promotes Plant Tolerance to Drought. Cells. 2021 Oct 25;10(11):2875. doi: 10.3390/cells10112875. PMID: 34831098; PMCID: PMC8616339.Mishra et al. (2021) Interplay between abiotic (drought) and biotic (virus) stresses in tomato plants. Mol Plant Pathol. 2021 Dec 30. doi: 10.1111/mpp.13172. Epub ahead of print. PMID: 34970822.Yang et al. (2020). PROTEIN PHOSPHATASE 95 Regulates Phosphate Homeostasis by Affecting Phosphate Transporter Trafficking in Rice. Plant Cell. 2020 Jan 9. pii: tpc.00685.2019. doi: 10.1105/tpc.19.00685.
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER, BiP protein is abundant under all growth conditions, but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER, BiP protein is abundant under all growth conditions, but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER, BiP protein is abundant under all growth conditions, but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Na(+)/H(+) exchanger 1 protein is involved in exchange of protons for cations such as sodium and potassium across membranes. Localized in tonoplast, possibly also to ER and Golgi. Alternative name: NHE-1, Na(+)/H(+) exchanger 1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Gossypium hirsutum, Populus euphratica, Ricinus communis, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NHX protein UniProt: Q68KI4, TAIR: At5g27150; chosen peptide is perfectly confirmed in AtNHX1 UniProt: Q0WVZ5, partially in AtNHX2, UniProt: Q56XP4, and not conserved in AtNHX3, UniProt: Q84WG1 and AtNHX4, UniProt:Q8S397 isoforms
Carmona-Salazar et al. (2021). Plasma and Vacuolar Membrane Sphingolipidomes: Composition and Insights on The Role of Main Molecular Species. Plant Physiol. 2021 Feb 11:kiab064. doi: 10.1093/plphys/kiab064. Epub ahead of print. PMID: 33570616.Cano-Ramirez et al. (2021) M. Plasma Membrane Fluidity: An Environment Thermal Detector in Plants. Cells. 2021 Oct 17;10(10):2778. doi: 10.3390/cells10102778. PMID: 34685758; PMCID: PMC8535034.Prinsi et al. (2020). Root Proteomic Analysis of Two Grapevine Rootstock Genotypes Showing Different Susceptibility to Salt Stress. Int J Mol Sci. 2020 Feb 6;21(3). pii: E1076. doi: 10.3390/ijms21031076.Gupta and Shaw (2020). Biochemical and molecular characterisations of salt tolerance components in rice varieties tolerant and sensitive to NaCl: the relevance of Na+ exclusion in salt tolerance in the species . Funct Plant Biol. 2020 Jul 30.doi: 10.1071/FP20089 Guo et al. (2018). Molecular Characterization of a Tonoplast Na+/H+ Antiporter from Iris Lactea. Preprints 2018, 2018090557 (doi: 10.20944/preprints201809.0557.v1).
Special application note:
Protocol for vacuolar membrane isolation can be found here.
PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across the cell membrane. Alternative names: PIP1-1, PIP1-2, PIP1-3, plasma membrane intrinsic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica sp., Jatropha curcas L. cv. Biji Jarak , Mesembryantheum crystallinum, Populus nigra, Populus trichocarpa, Raphanus sativus, Setaria viridis, Thellungiella salsuginea
Expected Species:
Brassica sp., Hordeum vulgare, Juglans regia, Nicotiana tabacum, Oryza sativa, Populus tremula, Triticum aestivum, Vicia fabaSpecies of your interest not listed? Contact us
Protein or membrane sample should be treated at 70 C for 10 min before loading on the gel.
Application Details:
1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
30,68 | 28 kDa
Not reactive in:
Fragaria sp., Spinacia oleracea
Selected references:
Chen et al. (2022) Elucidating the role of SWEET13 in phloem loading of the C4 grass Setaria viridis. Plant J. 2022 Feb;109(3):615-632. doi: 10.1111/tpj.15581. Epub 2021 Dec 12. PMID: 34780111.Jang et al. (2013). Twoaquaporins of Jatropha are regulated differentially during drought stress and subsequent recovery. J Plant Physiol. March 25.Lopez et al. (2013). Aquaporins And Leaf Hydraulics, Poplar Sheds New Light. Plant Cell Physiol. Sep 20.
Special application note:
Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient
PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across cell membrane. Alternative names: PIP1-1, PIP1-2, PIP1-3, plasma membrane intrinistic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica sp., Jatropha curcas L. cv. Biji Jarak , Mesembryantheum crystallinum, Populus nigra, Populus trichocarpa, Raphanus sativus, Thellungiella salsuginea
Expected Species:
Brassica sp., Hordeum vulgare, Juglans regia, Oryza sativa, Populus tremula, Triticum aestivum, Vicia faba Species of your interest not listed? Contact us
Protein or membrane sample should be treated at 70 C for 10 min before loading on the gel.
Application Details:
1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP.
Molecular Weight:
30,68 | 28 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient
PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across cell membrane. Alternative names: PIP1-1, PIP1-2, PIP1-3, plasma membrane intrinistic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica sp., Jatropha curcas L. cv. Biji Jarak , Mesembryantheum crystallinum, Populus nigra, Populus trichocarpa, Raphanus sativus, Thellungiella salsuginea
Expected Species:
Brassica sp., Hordeum vulgare, Juglans regia, Oryza sativa, Populus tremula, Triticum aestivum, Vicia fabaSpecies of your interest not listed? Contact us
Protein or membrane sample should be treated at 70 C for 10 min before loading on the gel.
Application Details:
1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin.
Molecular Weight:
30,68 | 28 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient
PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across the cell membrane. Alternative names: PIP1-1, PIP1-2, PIP1-3, plasma membrane intrinsic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica sp,, Jatropha curcas L, cv, Biji Jarak , Mesembryantheum crystallinum, Populus nigra, Populus trichocarpa, Raphanus sativus, Thellungiella salsuginea
Expected Species:
Brassica sp., Hordeum vulgare, Juglans regia, Nicotiana tabacum, Oryza sativa, Populus tremula, Triticum aestivum, Vicia fabaSpecies of your interest not listed? Contact us
Protein or membrane sample should be treated at 70 C for 10 min before loading on the gel.
Application Details:
To be determined by end user.
Purity:
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight 488.
Molecular Weight:
30,68 | 28 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
To be added when available. Antibody released in May 2023.
Special application note:
Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.DyLight 488 Amax = 493 nm, Emax = 519 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across the cell membrane, Alternative names: PIP1-1, PIP1-2, PIP1-3, plasma membrane intrinsic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica sp,, Jatropha curcas L, cv, Biji Jarak , Mesembryantheum crystallinum, Populus nigra, Populus trichocarpa, Raphanus sativus, Thellungiella salsuginea
Expected Species:
Brassica sp., Hordeum vulgare, Juglans regia, Nicotiana tabacum, Oryza sativa, Populus tremula, Triticum aestivum, Vicia fabaSpecies of your interest not listed? Contact us
Protein or membrane sample should be treated at 70 C for 10 min before loading on the gel,
Application Details:
To be determined by end user.
Purity:
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight 594.
Molecular Weight:
30,68 | 28 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
To be added when available. Antibody released in May 2023.
Special application note:
Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one g load per well should be sufficient.DyLight 594 has Amax = 593 nm, Emax = 618 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across the cell membrane. Alternative names: PIP1-1, PIP1-2, PIP1-3, plasma membrane intrinsic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica sp,, Jatropha curcas L, cv, Biji Jarak , Mesembryantheum crystallinum, Populus nigra, Populus trichocarpa, Raphanus sativus, Thellungiella salsuginea
Expected Species:
Brassica sp., Hordeum vulgare, Juglans regia, Nicotiana tabacum, Oryza sativa, Populus tremula, Triticum aestivum, Vicia fabaSpecies of your interest not listed? Contact us
Protein or membrane sample should be treated at 70 C for 10 min before loading on the gel.
Application Details:
To be determined by end user.
Purity:
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight 650.
Molecular Weight:
30,68 | 28 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
To be added when available. Antibody released in May 2023.
Special application note:
Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one g load per well should be sufficient.DyLight 650 has Amax = 652 nm, Emax = 672 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across cell membrane. Alternative names: PIP1-1, PIP1-2, PIP1-3, plasma membrane intrinistic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica sp., Jatropha curcas L. cv. Biji Jarak , Mesembryantheum crystallinum, Populus nigra, Populus trichocarpa, Raphanus sativus, Thellungiella salsuginea
Expected Species:
Brassica sp., Hordeum vulgare, Juglans regia, Oryza sativa, Populus tremula, Triticum aestivum, Vicia faba Species of your interest not listed? Contact us
Protein or membrane sample should be treated at 70 C for 10 min before loading on the gel.
Application Details:
1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP.
Molecular Weight:
30,68 | 28 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient
Catalase is an enzyme found in most living organisms which is catalazying decomposition of hydrogen peroxide to water and oxygen. In plant cells catalase is found in peroxisomes. This enzyme is involved in photorespiration and symbiotic nitrogen fixation.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Fragaria x ananassa,Arabidopsis thaliana, Aponogeton madagascariensis, Brassica napus, Brassica oleracea, Fragaria x ananassa, Hordeum vulgare, Lathyrus sativus, Lupinus albus, Lupinus luteus, Moniliophthora perniciosa, Musa acuminate, Musa paradisiaca L., Nicotiana bentamina, Nicotiana tabacum, Oryza sativa, Paulownia tomentosa, Picrorhiza kurroa, Pisum sativum, Plumbago zeylanica, Setaria italica L. P. Beauv, Solanum lycopersicum, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis vinifera
To obtain reactivity with Solanum lycopersicum urea gel needs to be apply. Please, contact us for more details.To decrease background signal this antibody needs to be incubated in PBS-T NOT TBS-T. For reference, check image in application example below.
Tokarz et al. (2021). Stem Photosynthesis-A Key Element of Grass Pea (Lathyrus sativus L.) Acclimatisation to Salinity. Int J Mol Sci. 2021 Jan 12;22(2):685. doi: 10.3390/ijms22020685. PMID: 33445673; PMCID: PMC7828162.Li et al. (2021) Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit. Hortic Res. 2021 Feb 1;8(1):31. doi: 10.1038/s41438-021-00470-w. PMID: 33518707; PMCID: PMC7848011.Wilmowicz et al (2021) EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers. International Journal of Molecular Sciences. 2021; 22(6):3001. https://doi.org/10.3390/ijms22063001Bapatla et al. (2021). Modulation of Photorespiratory Enzymes by Oxidative and Photo-Oxidative Stress Induced by Menadione in Leaves of Pea (Pisum sativum). Plants 10, no. 5: 987. https://doi.org/10.3390/plants10050987Adamiec et al. (2021). Fatty acid composition and cpDNA content in Arabidopsis thaliana mutants deprived of EGY1 protease. PHOTOSYNTHETICA 59 (4): 633-639, 2021, DOI 10.32615/ps.2021.053
Special application note:
This antibody is recognizing all three isoforms of Arabidopsis thaliana catalase. Catalase-2 is a main isoform expressed in leaf tissue and localized to peroxisomes. This antibody contains 0.1 % ProClin.
Lhcb4 (CP29) is a member of the family of chlorophyll a/b-binding proteins, which is conserved in higher plants and green algae. Lhcb4 is associated with Photosystem II serving both as a light-harvesting antenna protein as well as playing a role in photoprotective dissipation of excitation energy. Alternative name: CP29-like light-harvesting chlorophyll a/b binding protein chlor (IC)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Coccomyxa subellipsoidea, Ostreococcus tauri
Expected Species:
Bathycoccus prasinos, Micromonas sp., Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Lhcb4 (CP29) protein sequence from Ostreococcus tauri Q3B9U8
Lhcb5 is one of the 3 minor, highly conserved chlorophyll a/b-binding proteins associated with Photosystem II in plants and algae. As a part of the inner light-harvesting antenna it has been sugested to regulate (together with Lhcb4 and Lhcb6) the energy flow from the outer LHCII antenna to the PSII reaction center.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Coccomyxa subellipsoidea, Ostreococcus tauri
Expected Species:
Algae, Ostreococcus lucimarinus Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Lhcb4 (CP29) protein sequence from Ostreococcus taurii Q3B9U2
Iron-hydrogenase HydA2 is catalyzing reversible oxidation of molecular hydrogen. In Chlamydomonas the protein is present in low levels of 1 g/liter of culture.Synonyme: HYD1/1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Ostreococcus sp.Species of your interest not listed? Contact us
Immunogen:
Recombinant, full length Chlamydomonas reinhardtii HydA-2 Q8VZZ0
HydA1 (497aa) has a calculated MW of 53.1 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP has a calculated MW of 47.5 kDa and runs according to its size at about 48 kDa.HydA2 (505aa) has a calculated MW of 53.7 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP can only be estimated, since the cleavage site is known only from in silico analysis. It has a calculated MW of 47.3 kDa and should run in the gel also according to its size.This antibody is binding recombinant HydA1/2 protein.
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
53,7 | 48 kDa (after transit peptide is cleaved)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Jokel et al. (2020). Elimination of the flavodiiron electron sink facilitates long-term H2 photoproduction in green algae. Biotechnol Biofuels. 2019 Dec 5;12:280. doi: 10.1186/s13068-019-1618-1.Lindblad et al. (2019). CyanoFactory, a European consortium to develop technologies needed to advance cyanobacteria as chassis for production of chemicals and fuels. Algal Research, Volume 41, August 2019, 101510.Weiner at al (2018), Overcoming the expression barrier of the ferredoxin‑hydrogenase chimera in Chlamydomonas reinhardtii supports a linear increment in photosynthetic hydrogen output. Algal Research Volume 33, July 2018, Pages 310-315Wei et al. (2017). Light Intensity is Important for Hydrogen Production in NaHSO3-Treated Chlamydomonas reinhardtii. Plant Cell Physiol. 2017 Mar 1;58(3):451-457. doi: 10.1093/pcp/pcw216.Eilenberg et al. (2016). The dual effect of a ferredoxin-hydrogenase fusion protein in vivo: successful divergence of the photosynthetic electron flux towards hydrogen production and elevated oxygen tolerance. Biotechnol Biofuels. 2016 Aug 30;9(1):182. doi: 10.1186/s13068-016-0601-3. eCollection 2016.Liran et al. (2016). Microoxic Niches within the Thylakoid Stroma of Air-Grown Chlamydomonas reinhardtii Protect [FeFe]-Hydrogenase and Support Hydrogen Production under Fully Aerobic Environment. Plant Physiol. 2016 Sep;172(1):264-71. doi: 10.1104/pp.16.01063. Epub 2016 Jul 21.Reifschneider-Wegner et al. (2014). Expression of the [FeFe] hydrogenase in the chloroplast of Chlamydomonas reinhardtii. Int J of Hydrogen Energy, Volume 39, Issue 8, 6 March 2014, Pages 3657–3665.Pinto et al. (2013). Rubisco mutants of Chlamydomonas reinhardtii enhance photosynthetic hydrogen production. Appl Microbiol Biotechnol. May 7.Magneschi et al. (2012). A Mutant in the ADH1 Gene of Chlamydomonas reinhardtii Elicits Metabolic 2 Restructuring during Anaerobiosis. Plant Physiol. January 23 (ahead of print).
Special application note:
In Chlamydomonas HydA is present in low levels of 1 g/liter of culture. Therefore, an induction of cells by anaerobic adaptation or sulfur depravation (10 x higher amount than with anaerobic adaptation) is necessary for successful detection using this antibody. Methods of HydA induction are described in Hemschemeier et al. 2009.To detect HydA1/A2 in Chlamydomonas extracts amount loaded per well corresponds to 2 g of chlorophyll for sulfur deprived cells, where relatively much HydA1 is synthesized or corresponds to 2-4 g of artificially anaerobic induced cultures, where the HydA1 protein level is lower. This antibody is recognizing 1 ng of recombinant HydA protein.
KC1 is a regulatory K+ channel subunit that assembles with different inward-rectifying K+ channels to affect their activities. The protein is expressed in extremely low amounts, e.g. a few houndred per cell. Alternative names: AKT4, AtKC1, potassium channel TKC
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, At4g32650
In the work of Honsbein et al, 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning, ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems, In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell)
Application Details:
1 : 50 with 125I (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 l of sterile water
Molecular Weight:
75,5 | 75 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.
Special application note:
For detection images please, refer to the publication belowAntibody detects native and recombinant KC1
AKT1 is a highly selective inward-rectifying potassium channel located in cell membrane, that mediates potassium uptake by plant roots in response to low potassium conditions.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana AKT1 Q38998 , At2g26650
In the work of Honsbein et al, 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning, ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems, In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell)
Application Details:
1 : 50 with 125I (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 l of sterile water
Molecular Weight:
96,9 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Safiarian et al. (2015). Lost in traffic? The K+ channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves and lily pollen. Front. Plant Sci. | doi: 10.3389/fpls.2015.00047.Honsbein et al. (2009). A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis. The Plant Cell 21:2859-2877.
Special application note:
For detection images please, refer to the publication belowAntibody detects native and recombinant AKT1
Oxalate oxidase (EC=1.2.3.4) belongs to the family of oxidoreductases, and acts on the aldehyde or oxo group of donor with oxygen as acceptor. Those reactions are specific forglyoxylate and dicarboxylate metabolism. Alternative names: oxygen oxidoreductase, aero-oxalo dehydrogenase, oxalic acid oxidase.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Hordeum vulgare
Expected Species:
Triticum aestivum, Oryza sativa Species of your interest not listed? Contact us
Immunogen:
native oxalate oxidase have been purified from barley seedlings
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Application Details:
1 : 1000-1 : 4000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG in PBS, labelled with biotin.
Reconstitution:
For reconstitution please add 1 ml of sterile destilled water
Molecular Weight:
21 kDa
Not reactive in:
Arabidopsis thaliana
Special application note:
Biotin/IgG protein molar ratio (B/P) is approximately 6,6, No foreign proteins are added, Marker used for lebling is N-hydroxysuccinimidoBiotin
Aconitase is a single subunit enzyme of the tricarboxylic acid cycle (or Krebs cycle) in the mitochondria. A cytosolic isoform is also part of the glyoxylate cycle. Aconitase catalyzes the dehydration / hydration of citrate to iso-citrate, via cis-aconitate as an intermediate. The reaction is facilitated by an iron-sulphur cluster in the active site of the enzyme. The iron-sulphur cluster is somewhat unstable, especially under oxidative stress, and loss of the cofactor leads to degradation of the protein.Alternative names: ACO, citrate hydro-lyase 1,2,3
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana ACO1,ACO2 and ACO3 isoforms, Brassica oleracea, Solanum lycopersicum
Expected Species:
Cucurbita maxima, Hordeum vulgare, Nicotiana tabacum, Ricinus communis, Solanum tuberosum, Vitis vinifera, Oryza sativa, Zea mays, Picea sitcHensis, Populus trichocarpaSpecies of your interest not listed? Contact us
Immunogen:
Arabidopsis ACO1 (AT4G35830, Q42560), codon 120 – 898 (C-terminus), was cloned in fusion with a N-terminal 6xHis tag, and over-expressed in E. coli. All recombinant protein accumulated in inclusion bodies, which were purified by centrifugation and solubilised in 6 M guanidine-HCl. The protein was refolded by dilution in 100 mM Tris-HCl 8.5, 10% (v/v) glycerol, 2 mM dithiothreitol, and concentrated prior to immunisation.
The antibody recognises all three Arabidopsis aconitase isoforms (ACO1, ACO2 and ACO3, see Bernard et al 2009), Possible differences in affinity have not been precisely quantified, Sensitivity threshold is between 2 and 10 ng for WB / ECL (see figure), Antibodies will recognize aconitase isoforms in denaturing and native gel electrophoresis
Application Details:
1 : 5 000 -1 : 10 000 (WB), At higher concentrations the antibody binds aspecifically resulting in non-specific signals around 60 kDa, including Rubisco subunits
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
98 kDa, Note that ACO1, ACO2 and ACO3 cannot be distinguished in size by standard SDS-PAGE,
Not reactive in:
Chlamydomonas reinhardii, cyanobacteria
Selected references:
Pascual et al (2021). ACONITASE 3 is part of the ANAC017 transcription factor-dependent mitochondrial dysfunction response, Plant Physiology, 2021;, kiab225, https://doi.org/10.1093/plphys/kiab225Przybyla-Toscano et al. (2021) Protein lipoylation in mitochondria requires Fe-S cluster assembly factors NFU4 and NFU5. Plant Physiol. 2021 Oct 28:kiab501. doi: 10.1093/plphys/kiab501. Epub ahead of print. PMID: 34718778.Rurek et al. (2018). Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought Involves Impaired Coordination of Transcriptomic and Proteomic Response and Regulation of Various Multifunctional Proteins. Preprints 2018, 2018010276 (doi: 10.20944/preprints201801.0276.v1).Seti n et al. (2014). Root phosphoenolpyruvate carboxylase and NAD-malic enzymes activity increase the ammonium-assimilating capacity in tomato. J Plant Physiol. 171:49-63.Birke et al. (2012). Cysteine biosynthesis, in concert with a novel mechanism, contributes to sulfide detoxification in mitochondria of Arabidopsis thaliana. Biochem J. May 2, ahead of print.
Special application note:
Arabidopsis expresses three highly similar aconitase isozymes (ACO1/ AT4G35830, ACO2/AT4G26970 and ACO3/AT2G05710), of which ACO1 is the cytosolic isoform, while ACO2 and ACO3 are predominantly located in the mitochondria (Arnaud et al 2007, Bernard et al 2009), The combined abundance and activity of the mitochondrial aconitases is about 3 times higher than the cytosolic pool (Bernard et al 2009),The Arabidopsis isoforms are more similar in amino acid sequence to mammalian iron-regulatory proteins (IRP-1) than to the mammalian and yeast mitochondrial aconitases
The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Digitaria sanguinalis, Echinochloa crus-galli, Pinus strobus L.
Expected Species:
Algae, Dicots, Mosses Species of your interest not listed? Contact us
Immunogen:
BSA-conjugated synthetic peptide derived from Arabidopsis thaliana At1g29910 (Lhcb1.1), At1g29920 (Lhcb1.2), At1g29930 (Lhcb1.3, most expressed), At2g34430 (Lhcb1.4), and At2g34420 (Lhcb1.5)
This Lhcb1 antibody is directed specifically against the Arabidopsis Lhcb1 gene products, for those that would prefer higher specific activity over broader specificity offered by Agrisera older Lhcb1 antibody, AS01 004Protein is processed into mature form (Jansson 1999).
Application Details:
1 : 2500-1 : 5000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
28 | 25 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Wang et al. (2020). Post-translational coordination of chlorophyll biosynthesis and breakdown by BCMs maintains chlorophyll homeostasis during leaf development. Nat Commun. 2020; 11: 1254. Pralon et al. (2019). Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019 Jun 20;2:220. doi: 10.1038/s42003-019-0477-4. Lal et al. (2018). The Receptor-like Cytoplasmic Kinase BIK1 Localizes to the Nucleus and Regulates Defense Hormone Expression during Plant Innate Immunity. Cell Host Microbe. 2018 Apr 11;23(4):485-497.e5. doi: 10.1016/j.chom.2018.03.010.Tamburino et al. (2017). Chloroplast proteome response to drought stress and recovery in tomato (Solanum lycopersicum L.). BMC Plant Biol. 2017 Feb 10;17(1):40. doi: 10.1186/s12870-017-0971-0.Fristedt et al. (2017). PSB33 sustains photosystem II D1 protein under fluctuating light conditions. Journal of Experimental Botany doi:10.1093/jxb/erx218.Hartings et al. (2017). The DnaJ-Like Zinc-Finger Protein HCF222 Is Required for Thylakoid Membrane Biogenesis in Plants. Plant Physiol. 2017 Jul;174(3):1807-1824. doi: 10.1104/pp.17.00401.Correa-Galvis et al. (2016). PsbS interactions involved in the activation of energy dissipation in Arabidopsis. Nature Plants 2, Article number: 15225 (2016) doi:10.1038/nplants.2015.225Longoni et al. (2015). Phosphorylation of the Lhcb2 isoform of Light Harvesting Complex II is central to state transitions. Plant Physiol. 2015 Oct 5. pii: pp.01498.2015.Wientjes et al (2013). LHCII is an antenna of both photosystems after long-term acclimation. BBA, Jan 6.
Special application note:
A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467–9476
Plant MnSOD catalyzes the dismutation of superoxide to molecular oxygen and water. One subunit of the homotetramer complex binds 1 Mn cofactor. Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H,O,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD. Synonymes: MSD1, SODA, SOD3
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Gossipium mexicanum, Hordeum vulgare, Musa acuminata, Picea sitcHensis, Populus balsamifera sub. trichocarpa, Raphanus sativus, Solanum tuberosum, Triticum aestivum, Vitis vinifera, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from available MnSOD sequences in di and monocotyl plants including Arabidopsis thaliana O81235, At3g10920
Bastow et al. (2018). Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds. Plant Physiol. 2018 Jul;177(3):1267-1276. doi: 10.1104/pp.18.00478.Bal žov et al. (2018). Zinc oxide nanoparticles phytotoxicity on halophyte from genus Salicornia. Plant Physiol Biochem. 2018 Sep;130:30-42. doi: 10.1016/j.plaphy.2018.06.013Rurek et al. (2018). Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe DBal žov rought Involves Impaired Coordination of Transcriptomic and Proteomic Response and Regulation of Various Multifunctional Proteins. Preprints 2018, 2018010276 (doi: 10.20944/preprints201801.0276.v1).Schimmeyer et al. (2016). L-Galactono-1,4-lactone dehydrogenase is an assembly factor of the membrane arm of mitochondrial complex I in Arabidopsis. Plant Mol Biol. 2016 Jan;90(1-2):117-26. doi: 10.1007/s11103-015-0400-4. Epub 2015 Oct 31.Yin et al. (2016). Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.Vuleta et al. (2016). Adaptive flexibility of enzymatic antioxidants SOD, APX and CAT to high light stress: The clonal perennial monocot Iris pumila as a study case. Plant Physiol Biochem. 2016 Mar;100:166-73. doi: 10.1016/j.plaphy.2016.01.011. Epub 2016 Jan 19Dmitrović et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64.Dimkovikj and Van Hoewyk (2014). Selenite activates the alternative oxidase pathway and alters primary metabolism in Brassica napus roots: evidence of a mitochondrial stress response. BMC Plant Biol. 2014 Sep 30;14:259. doi: 10.1186/s12870-014-0259-6.Parys et al. (2014). Metabolic Responses to Lead of Metallicolous and Nonmetallicolous Populations of Armeria maritima. Arch Environ Contam Toxicol. 2014 Jul 29.Momčilović et al. (2014). Improved procedure for detection of superoxide dismutase isoforms in potato, Solanum tuberosum L. Acta Physiologiae Plantarum, August 2014, Volume 36, Issue 8, pp 2059-2066.
Special application note:
Freshly prepared reducing agent like DTT needs to be used in a sample buffer, Otherwise MnSOD will migrate at 50 kDa
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Buffer for extraction of AGO proteins: Paudel et al. 2018. The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.TCA acetone precipitation method
Meng et al. (2022) The novel activity of Argonautes in intron splicing: A transcriptome-wide survey in plants. J Plant Physiol. 2022 Jan 31;270:153632. doi: 10.1016/j.jplph.2022.153632. Epub ahead of print. PMID: 35114616.Cabezas-Fuster et al. (2022). Missplicing suppressor alleles of Arabidopsis PRE-MRNA PROCESSING FACTOR 8 increase splicing fidelity by reducing the use of novel splice sites. Nucleic Acids Res. 2022 Jun 10;50(10):5513-5527. doi: 10.1093/nar/gkac338. PMID: 35639749; PMCID: PMC9177961.Delenko et al. (2022) MicroRNA biogenesis and activity in plant cell dedifferentiation stimulated by cell wall removal. BMC Plant Biol. 2022 Jan 3;22(1):9. doi: 10.1186/s12870-021-03323-9. PMID: 34979922; PMCID: PMC8722089. (immunofluorescence)Oliver & Martinez. (2021) Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis. Plant Reprod. 2021 Nov 23. doi: 10.1007/s00497-021-00434-z. Epub ahead of print. PMID: 34812935.Dalmadi et al. (2021) Controlled RISC loading efficiency of miR168 defined by miRNA duplex structure adjusts ARGONAUTE1 homeostasis. Nucleic Acids Res. 2021 Dec 16;49(22):12912-12928. doi: 10.1093/nar/gkab1138. PMID: 34850097; PMCID: PMC8682782.
Special application note:
Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.To detect AGO1 in Nicotiana benthamiana, please inquire.Recommended for detection of AGO1: extreme low femtogram range chemiluminescent detection reagent
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).This antibody is directly conjugated to Alkaline Phosphatase (ALP).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.TCA acetone precipitation method
Application Details:
1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP.
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).This antibody is directly conjugated to Biotin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conugated N-terminal peptide of Arabidopsis thaliana AGO1 UniProt: O04379, TAIR:At1g48410.
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.
Application Details:
1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin.
Molecular Weight:
116,4 | 130 kDa
Not reactive in:
Chlamydomonas reinhardtii
Special application note:
Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.TCA acetone total protein precipitation method
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC), This protein complex is responsible for the gene silencing (RNAi).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Nicotiana benthamiana
Expected Species:
Brassica pekinensis, Capsella rubella, Glycine max, Malus domestica, Pisum sativum, Ricinus communis, Solanum tuberosum, Zea mays, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated, N-terminal peptide of Arabidopsis thaliana AGO1 O04379, At1g48410
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.
Application Details:
To be determined by end user.
Purity:
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight 488.
Molecular Weight:
116,4 | 130 kDa
Not reactive in:
Chlamydomonas reinhardtii
Selected references:
To be added when available. Antibody released in May 2023.
Special application note:
Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U,TCA acetone total protein precipitation method.DyLight 488 Amax = 493 nm, Emax = 519 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). This antibody is directly conjugated to Horseradish Peroxidase (HRP).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Nicotiana benthamiana
Expected Species:
Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated, N-terminal peptide of Arabidopsis thaliana AGO1 O04379, At1g48410
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.
Application Details:
To be determined by end user.
Purity:
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight 594.
Molecular Weight:
116,4 | 130 kDa
Not reactive in:
Chlamydomonas reinhardtii
Selected references:
To be added when available. Antibody released in May 2023.
Special application note:
Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U,TCA acetone total protein precipitation method.DyLight 594 has Amax = 593 nm, Emax = 618 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC), This protein complex is responsible for the gene silencing (RNAi),This antibody is directly conjugated to Horseradish Peroxidase (HRP).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Nicotiana benthamiana
Expected Species:
Brassica pekinensis, Capsella rubella, Glycine max, Malus domestica, Pisum sativum, Ricinus communis, Solanum tuberosum, Zea mays, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated, N-terminal peptide of Arabidopsis thaliana AGO1 O04379, At1g48410
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.
Application Details:
To be determined by end user.
Purity:
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight 650.
To be added when available. Antibody released in May 2023.
Special application note:
Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U, TCA acetone total protein precipitation method.DyLight 650 has Amax = 652 nm, Emax = 672 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).This antibody is directly conjugated to Horseradish Peroxidase (HRP).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.
Application Details:
1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP.
This blocking peptide can be used as a control to neutralize AGO1 | argonaute 1 before immunolocalization or western blot. Furter details are provided below.AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Due to its low molecular weight, this peptide is not suitable for separation on a SDS gel,
Reconstitution:
For reconstitution please add sterile water
Molecular Weight:
1716,9 Da
Special application note:
Estimation of the ratio between antibodies and peptide : In neutralisation assay use 9,16 g of argonaute 1 peptide for 10 ml of reaction volume. In case of any questions please contact support@agrisera.com
CBP20 (Nuclear cap-binding protein subunit 2)is a component of the cap-binding complex (CBC), involved in various processes such as pre-mRNA splicing and RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs). Alternative names: 20 kDa nuclear cap-binding protein, NCBP 20 kDa subunit
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Glycne max, Hordeum vulgare, Lotus corniculatus, Nicotiana tabacum, Oryza sativa, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Zea mays Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide, derived with Arabidopsis thaliana CBP20 protein Q9xFD1, At5g44200
CBP80 is a component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing. Alternative names: 80 kDa nuclear cap-binding protein, NCBP 80 kDa subunit, abscisic acid-hypersensitive protein 1, ABA-hypersensitive protein 1, Protein ENSALADA
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Serrate RNA effector molecule is required for proper processing of primary miRNAs to miRNA. Also critical for the accumulation of the trans-acting small interfering RNA (ta-siRNA).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Suggested blotting conditions: 8% gel, tank blotting, 200 mA/ 1h to nitrocellulose membrane
Application Details:
1 : 500 (IL), 1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
81 | 80 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Li et al. (2021). In vitro Reconstitution Assays of Arabidopsis 20S Proteasome. Bio-protocol 11(7): e3967. DOI: 10.21769/BioProtoc.3967.Li et al. (2020). Apple SERRATE negatively mediates drought resistance by regulating MdMYB88 and MdMYB124 and microRNA biogenesis. Hortic Res. 2020 Jul 1;7:98.doi: 10.1038/s41438-020-0320-6. (ChIP)Li et al. (2019). Global co-transcriptional splicing in Arabidopsis and the correlation with splicing regulation in mature RNAs. Mol Plant. 2019 Nov 20. pii: S1674-2052(19)30367-3. doi: 10.1016/j.molp.2019.11.003.Wang et al. (2019). The PROTEIN PHOSPHATASE4 Complex Promotes Transcription and Processing of Primary microRNAs in Arabidopsis. Plant Cell. 2019 Feb;31(2):486-501. doi: 10.1105/tpc.18.00556. de Francisco Amorim et al. (2018). The U1 snRNP Subunit LUC7 Modulates Plant Development and Stress Responses via Regulation of Alternative Splicing. Plant Cell. 2018 Nov;30(11):2838-2854. doi: 10.1105/tpc.18.00244. Epub 2018 Oct 11.
The 22 kDa PsbS protein of photosystem II functions in the regulation of photosynthetic light harvesting. Along with a low thylakoid lumen pH and the presence of de-epoxidized xanthophylls, PsbS is necessary for photoprotective thermal dissipation of excess absorbed light energy in plants, measured as non-photochemical quenching of chlorophyll fluorescence. Synonymes: NPQ4 (NONPHOTOCHEMICAL QUENCHING).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Chlamydomonas reinhardtii, Cucumis sativus, Medicago truncatula, Physcomitrium patens, Picea sitchensis, Pinus radiata, Pinus taeda, Populus balsamifera, Solanum lycopersicum, Tarenaya hassleriana, Zosteria marina, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide located in solubilized part of the protein, derived from available di- and monocot PsbS sequences, including Arabidopsis thaliana UniProt:Q9XF91, TAIR:At1g44575
This product can be sold containing proclin if requested
Application Details:
1 : 2000 - 1: 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
28 | 22 kDa for Arabidopsis thaliana
Not reactive in:
Lobosphaera incisa
Selected references:
Jiang et al. (2020). Plastid chaperone HSP90C guides precursor proteins to the SEC translocase for thylakoid transport. J Exp Bot. 2020 Aug 27;eraa399.doi: 10.1093/jxb/eraa399. Barbato et al. (2020). Higher Order Photoprotection Mutants Reveal the Importance of ?pH-dependent Photosynthesis-Control in Preventing Light Induced Damage to Both Photosystem II and Photosystem I. Sci Rep . 2020 Apr 21;10(1):6770. doi: 10.1038/s41598-020-62717-1.Nikkanen et al. (2018). Multilevel regulation of non-photochemical quenching and state transitions by chloroplast NADPH-dependent thioredoxin reductase. Physiol Plant. 2018 Dec 22. doi: 10.1111/ppl.12914.Chen et al. (2018). Exogenous melatonin enhances salt stress tolerance in maize seedlings by improving antioxidant and photosynthetic capacity. Physiol Plant. 2018 Apr 6. doi: 10.1111/ppl.12737.Glowacka et al. (2018). Photosystem II Subunit S overexpression increases the efficiency of water use in a field-grown crop. Nat Commun. 2018 Mar 6;9(1):868. doi: 10.1038/s41467-018-03231-x.
Papain (EC=3.4.22.2) is a cysteine protease which belongs to peptidase C1 family. Can cause an allergic reaction in humans. Alternative names: Papaya proteinase I, allergen= Car p 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Carica papaya
Expected Species:
Carica papaya
Immunogen:
native papain isolated and purified from Carica papaya
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography
Application Details:
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG in PBS.
Reconstitution:
For reconstitution add 1 ml of sterile destilled water
Molecular Weight:
38,9 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Papain (EC=3.4.22.2) is a cysteine protease which belongs to peptidase C1 family. Can cause an allergic reaction in humans. Alternative names: Papaya proteinase I, allergen= Car p 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Carica papaya
Expected Species:
Carica papaya
Immunogen:
Native papain isolated and purified from Carica papaya
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Biotin/IgG protein molar ration is approximately 6,2, No foreign proteins are added
Application Details:
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG in PBS.
Reconstitution:
For reconstitution add 0,5 ml of sterile destilled water
Molecular Weight:
38,9 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is labelled with biotin using N-hydroxysuccinimidobiotin, Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Papain (EC=3.4.22.2) is a cysteine protease which belongs to peptidase C1 family. Can cause an allergic reaction in humans. Alternative names: Papaya proteinase I, allergen= Car p 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Carica papaya
Expected Species:
Carica papaya
Immunogen:
native papain isolated and purified from Carica papaya
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran
Application Details:
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Purity:
Immunogen affinity purified IgG in PBS.
Reconstitution:
For reconstitution add 0,5 ml of sterile destilled water
Molecular Weight:
38,9 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Ananas comosus
Expected Species:
Ananas comosus
Immunogen:
native bromelain isolated and purified from Ananas comosus
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography
Application Details:
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG in PBS.
Reconstitution:
For reconstitution add 1 ml of sterile destilled water
Molecular Weight:
39 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Ananas comosus
Expected Species:
Ananas comosus
Immunogen:
native bromelain isolated and purified from Ananas comosus
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Biotin/IgG protein molar ration is approximately 6,2, No foreign proteins are added
Application Details:
1 : 5000-1 : 50 000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG in PBS.
Reconstitution:
For reconstitution add 1 ml of sterile destilled water
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is labelled with biotin using N-hydroxysuccinimidobiotin, Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Ananas comosus
Expected Species:
Ananas comosus
Immunogen:
native bromelain isolated and purified from Ananas comosus
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran
Application Details:
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Purity:
Immunogen affinity purified IgG in PBS.
Reconstitution:
For reconstitution add 0,5 ml of sterile destilled water
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Canavalia ensiformis
Expected Species:
Arabidopsis thaliana, Glycine max, Ricinus communis Species of your interest not listed? Contact us
Immunogen:
urease isolated and purified from Canavalia ensiformis (common jack-beans)
Applications:
ELISA (ELISA), Western blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography
Application Details:
1 : 1000-1 : 30 000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG in PBS.
Reconstitution:
For reconstitution add 1 ml of sterile destilled water
Molecular Weight:
91 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Canavalia ensiformis
Expected Species:
Arabidopsis thaliana, Glycine max, Ricinus communis Species of your interest not listed? Contact us
Immunogen:
urease isolated and purified from Canavalia ensiformis (common jack-beans)
Applications:
ELISA (ELISA), Western blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Biotin/IgG protein molar ration is approximately 6,2, No foreign proteins are added
Application Details:
1 : 1000-1 : 30 000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG in PBS.
Reconstitution:
For reconstitution add 1 ml of sterile destilled water
Molecular Weight:
91 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is labelled with biotin using N-hydroxysuccinimidobiotin, Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Peanut (Arachis hypogaea) belongs to the legume family. Dietary substances from peanuts can be a cause of allergi reaction in estimated 0.4-0.6% of population.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Soybean (Glycine max) is a plant from the legume family with a broad use in the food industry.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Wheat (Triticum aestivum) gluten consists of storage proteins known as gliadin and glutenin. Some individuals in population are gluten sensitive
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 1 g/ l
Storage Temp:
Store at 4 C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Wheat gluten
Expected Species:
Wheat gluten
Immunogen:
Triticum aestivum flour protein extract
Applications:
ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
Saccharomyces cerevisiae Rnr3 is catalyzing the biosynthesis of deoxyribonucelaotides. Alternative names: Ribonucleotide reductase large subunit 2, ribonucleotide reductase DNA damage-inducible regulatory subunit 2, ribonucleotide reductase R1 subunit 2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Saccharomyces cerevisiae
Expected Species:
Saccharomyces cerevisiae
Immunogen:
KLH-conjugated synthetic peptide derived from Saccharomyces cerevisiae Rnr3 protein sequence UniProt: P21672
Load per well was approx 3x10^6 cells (of a total extract)
Application Details:
1 : 500-1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
97,5 | 98 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Ajazi et al. (2021) Endosomal trafficking and DNA damage checkpoint kinases dictate survival to replication stress by regulating amino acid uptake and protein synthesis. Dev Cell. 2021 Sep 27;56(18):2607-2622.e6. doi: 10.1016/j.devcel.2021.08.019. Epub 2021 Sep 16. PMID: 34534458.Cerritelli et al. (2020). High density of unrepaired genomic ribonucleotides leads to Topoisomerase 1-mediated severe growth defects in absence of ribonucleotide reductase. Nucleic Acids Res Sampaio-Marques et al. (2019). ?-Synuclein toxicity in yeast and human cells is caused by cell cycle re-entry and autophagy degradation of ribonucleotide reductase 1. Aging Cell. 2019 Aug;18(4):e12922. doi: 10.1111/acel.12922.Schmidt et al. (2019). Inactivation of folylpolyglutamate synthetase Met7 results in genome instability driven by an increased dUTP/dTTP ratio. Nucleic Acids Res. 2019 Oct 24. pii: gkz1006. doi: 10.1093/nar/gkz1006.Lafuente-Barquero et al. (2017). The Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damage. PLOS Genetics, December 27, 2017, doi.org/10.1371/journal.pgen.1007136
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Algae, Chlamydomonas reinhardtii, Hordeum vulgare, Malus domestica, Mesembryanthemum sp., Oryza sativa, Petunia sp.,Phaseolus sp. , Physcomitrium patens, Pteris vittata (fern), Ricinus communis, Thellungiella sp., Zea mays, Vitis vinifera Bull frog, Chicken, Bovine, Drosophila melanogaster, Human, Mouse, Rat Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).
V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background.
Application Details:
1 : 1000-1 : 3000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 300 l of sterile water
Molecular Weight:
26 | 31 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
McLoughlin et al. (2012). TheSnf1-relatedproteinkinasesSnRK2.4 andSnRK2.10 areinvolved inmaintenance ofrootsystemarchitecture duringsaltstress. Plant J. June 2012.
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).
V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background.
Application Details:
1: 600 (IF), 1 : 1000-1 : 3000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 l of sterile water
Molecular Weight:
26 | 31 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii
Expected Species:
Acinetobacter baumannii, Enterobacter agglomerans, Klebsiella pneumoniae, Klebsiella oxytoca, Kluyvera ascorbata, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Worthington, Staphylococcus aureus, Serratia sp., Shigella flexneri Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from known aadA1 protein sequences
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Lauersen et a. (2018). Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii. Metab Eng. 2018 Jul 12;49:116-127. doi: 10.1016/j.ymben.2018.07.005.
Special application note:
This product can be sold containing ProClin if requested
aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection. This antibody is directly conjugated to ALP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii
Expected Species:
Acinetobacter baumannii, Enterobacter agglomerans, Klebsiella pneumoniae, Klebsiella oxytoca, Kluyvera ascorbata, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Worthington, Staphylococcus aureus, Serratia sp., Shigella flexneriSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from known aadA1 protein sequences
aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection. This antibody is directly conjugated to Biotin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii
Expected Species:
Acinetobacter baumannii, Enterobacter agglomerans, Klebsiella pneumoniae, Klebsiella oxytoca, Kluyvera ascorbata, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Worthington, Staphylococcus aureus, Serratia sp., Shigella flexneriSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from known aadA1 protein sequences
aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection. This antibody is directly conjugated to HRP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii
Expected Species:
Acinetobacter baumannii, Enterobacter agglomerans, Klebsiella pneumoniae, Klebsiella oxytoca, Kluyvera ascorbata, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Worthington, Staphylococcus aureus, Serratia sp., Shigella flexneriSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from known aadA1 protein sequences
F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Chlamydomonas reinhardtii
Expected Species:
Algae, Cannabis sativa, Glycine max, Hordeum vulgare, Oryza sativa, Ostreococcus tauri, Physcomitrium patens, Pinus thunbergii, Pisum sativum, Populus alba, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptides derived from AtpH subunit c of Arabidopsis thaliana UniProt: P56760, TAIR: AtCg00140 and Chlamydomonas reinhardtii UniProt: Q37304
Please note that increased incubation at 95 C (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e,g, 5 or 6 subunits) may occur
Application Details:
1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
8 kDa (for Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Schulz et al. (2017). Molecular architecture of the N-type ATPase rotor ring from Burkholderia pseudomallei. EMBO Rep. 2017 Apr;18(4):526-535. doi: 10.15252/embr.201643374.
Special application note:
This product can be sold containing ProClin if requested
Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. A killifish is a large family of various oviparous (egg-laying) cyprinodontiform fish including 1270 different species.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Chandra et al. (2012). Sustained high temperature increases the vitellogenin response to 17 alpha-ethynylestradiol in mummichog (Fundulus heteroclitus). Aquatic toxicology.
Special application note:
Chosen peptide sequence is also conserved in several fish species including: DQ202278.1 HSC70 Fundulus, DQ202279.1 HSP70-1 Fundulus, DQ202280.1 HSP70-2 Fundulus, BT059361.1 HSC70 Salmo salar (atlantic salmon), AB092839.2 HSP70 Carassius auratus (goldfish), BC056709.1 HSP70 Danio rerio (zebrafish) and other animal HSP70 proteins
Iron-hydrogenase HydA2 is catalyzing reversible oxidation of molecular hydrogen. Chlamydomonas the protein is present in low levels of 1 g/liter of culture. Synonymnes: HYD2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii, recombinant HydA2 expressed in E.coli
Expected Species:
Ostreococcus sp. Species of your interest not listed? Contact us
Immunogen:
Recombinant, full length Chlamydomonas reinhardtii HydA-2 UniProt: Q8VZZ0
HydA2 (505aa) has a calculated MW of 53.7 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP can only be estimated, since the cleavage site is known only from in silico analysis. It has a calculated MW of 47.3 kDa and should run in the gel also according to its size.HydA1 (497aa) has a calculated MW of 53.1 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP has a calculated MW of 47.5 kDa and runs according to its size at about 48 kDa
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
53,7 | 47,3 kDa (after a signal peptide is cleaved)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
In Chlamydomonas HydA is present in low levels of 1 g/liter of culture. Therefore, an induction of cells by anaerobic adaptation or sulfur depravation (10 x higher amount than with anaerobic adaptation) is necessary for successful detection using this antibody. Methods of HydA induction are described in Hemschemeier et al. 2009.To detect HydA in Chlamydomonas extracts amount loaded per well corresponds to 2 g of chlorophyll for sulfur deprived cells, where relatively much HydA1 is synthesized or corresponds to 2-4 g of artificially anaerobic induced cultures, where the HydA1 protein level is lower.This antibody is recognizing 1 ng of recombinant HydA protein.
c-Myc is derived from Myc proto-oncogene protein. A short peptide located between amino acids 408-420 is used as an epitope tag, that is easily recognized by tag specific antibodies. This is a universal detection reagent which will allow detection of any tag containing protein.This antibody is directly conjugated to Biotin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized antibodies at -20 °C and reconstituted antibodies at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
c-Myc
Expected Species:
c-Myc
Immunogen:
KLH-conjugated peptide, sequence MEQKLISEEDLNE, human c-Myc UniProt: Q6LBK7
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water, add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Based on IEP, this antibody reacts with: rabbit IgG heavy chains and light chains on all rabbit immunoglobulins
Immunogen:
Purified Rabbit IgG, whole molecule,
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Immunogen affinity purified using solid phase rabbit IgG.
Reconstitution:
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Spin centrifuge shortly to remove any particles. Prepare fresh working dilutions daily
Not reactive in:
Non-immunoglobulin rabbit serum proteins
Selected references:
Lim et al (2022). Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13(1):652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Miklankova et al. (2022) HYPK promotes the activity of the Nalfa-acetyltransferase A complex to determine proteostasis of nonAc-X2/N-degron-containing proteins. Sci Adv. 2022 Jun 17;8(24):eabn6153. doi: 10.1126/sciadv.abn6153. Epub 2022 Jun 15. PMID: 35704578; PMCID: PMC9200280.Hofmann, Wienkoop & Luthje (2022) Hypoxia-Induced Aquaporins and Regulation of Redox Homeostasis by a Trans-Plasma Membrane Electron Transport System in Maize Roots. Antioxidants (Basel). 2022 Apr 25;11(5):836. doi: 10.3390/antiox11050836. PMID: 35624700; PMCID: PMC9137787.Bychkov et al. (2022) The role of PAP4/FSD3 and PAP9/FSD2 in heat stress responses of chloroplast genes. Plant Sci. 2022 Sep;322:111359. doi: 10.1016/j.plantsci.2022.111359. Epub 2022 Jun 20. PMID: 35738478.Vitale et al. (2021) Light Spectral Composition Influences Structural and Eco-Physiological Traits of Solanum lycopersicum L. cv. 'Microtom' in Response to High-LET Ionizing Radiation. Plants (Basel). 2021 Aug 23;10(8):1752. doi: 10.3390/plants10081752. PMID: 34451797; PMCID: PMC8399554.
Special application note:
Concentration: 1.0 mg/ml.Antibody is provided in: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1% BSA (w/v), Protease IgG free, 0.1 % (v/v) ProClin 150.Affinity purified antibody is >95% pure, according to SDS-PAGE.This antibody can used on a very wide range of samples from various species including many model plants, algae, diatoms and bacteria.
Immunogen affinity purified using solid phase rabbit IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Migocka et al. (2018). Cucumber metal tolerance protein 7 (CsMTP7) is involved in the accumulation of Fe in mitochondria under Fe excess. Plant J. 2018 Jun 22. doi: 10.1111/tpj.14006. Tong et al. (2018). Delivery of siRNA in vitro and in vivo using PEI-capped porous silicon nanoparticles to silence MRP1 and inhibit proliferation in glioblastoma. J Nanobiotechnology. 2018 Apr 13;16(1):38. doi: 10.1186/s12951-018-0365-y. Nikkanen et al. (2018). Regulation of chloroplast NADH dehydrogenase-like complex by NADPH-dependent thioredoxin system. CSH, BioRixiv. doi.org/10.1101/261560. Gzyl et al. (2017). Gamma-tubulin distribution and ultrastructural changes in root cells of soybean (Glycine max L.) seedlings under cadmium stress. Environmental and Experimental Botany, Vol 143, Nov 2017, Pages 82-90. Kamies et al. (2017). A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef. Proteomes. 2017 Nov 15;5(4). pii: E32. doi: 10.3390/proteomes5040032. Niederhuber et al. (2017). Super-resolution microscopy of the -carboxysome reveals a homogenous matrix. Mol Biol Cell. 2017 Aug 9. pii: mbc.E17-01-0069. doi: 10.1091/mbc.E17-01-0069. This antibody is listed in first 7000 most published antibodies in the world by CiteAB report.
Special application note:
Concentration: 1.0 mg/ml. Antibody is provided in: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1% BSA (w/v), Protease IgG free, 0.1 % (v/v) Kathon CG. Affinity purified antibody is > 95 % pure, according to SDS-PAGE.
Goat anti-chicken IgY (H&L) is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to chicken IgY in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Chicken IgY heavy and light chains (H&L).
Immunogen:
Purified chicken IgY, whole molecule
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Antibody binds to:heavy chains on chicken IgYlight chains on all chicken immunoglobulinsNo reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.Chicken immunoglobulin is often called hen or chicken IgG, however it is derived from egg yolk, therefore IgY.
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Bindari et al. (2020). Methods to prevent PCR amplification of DNA from non-viable virus were not successful for infectious laryngotracheitis virus. PLoS One. 2020 May 22;15(5):e0232571. doi: 10.1371/journal.pone.0232571. PMID: 32442180; PMCID: PMC7244108.Levitan et al. (2019). Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17316-17322. doi: 10.1073/pnas.1906726116.Heard et al. (2015). Identification of Regulatory and Cargo Proteins of Endosomal and Secretory Pathways in Arabidopsis thaliana by Proteomic Dissection. Mol Cell Proteomics. 2015 Jul;14(7):1796-813. doi: 10.1074/mcp.M115.050286. Epub 2015 Apr 21.Huang et al. (2015). Effects of exogenous salicylic acid on the physiological characteristics of Dendrobium officinale under chilling stress. Plant Growth Regulation pp 1-10.
Special application note:
Concentration: 1.0 mg/mlThis HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative.
Donkey anti-sheep IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all sheep immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For storage at -20 °C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Donkey
Species Reactivity:
Sheep IgG heavy chains and light chains on all sheep immunoglobulins
Immunogen:
Purified sheep IgG, whole molecule
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Not reactive in:
non-immunoglobulin sheep serum proteins based in immunoelectrophoresis
Special application note:
Antibody concentration is 1 mg/ml and it has been purified by antigen-specific chromatographyHRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of ProClin 150 is used as preservative.For blocking: BSA and nonfat milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Rabbit anti-goat IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all goat immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Rabbit
Species Reactivity:
Goat IgG heavy and light chains on all goat immunoglobulins
Immunogen:
Purified goat IgG, whole molecule
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
For blocking BSA and non-fat milk is recommended to be replaced by other blocking reagents, like donkey serum or commercial formulations which are free from bovine IgG.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Not reactive in:
Non-immunoglobulin goat serum proteins based in immunoelectrophoresis
Selected references:
Sinclair et al. (2017) Etiolated Seedling Development Requires Repression of Photomorphogenesis by a Small Cell-Wall-Derived Dark Signal. Curr Biol. 2017 Nov 20;27(22):3403-3418.e7. doi: 10.1016/j.cub.2017.09.063.
Special application note:
Concentration: 1.0 mg/mlHRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free with 0.1 % (v/v) of ProClin 150 as preservative.
Rabbit anti-goat IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all goat immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized material at 2-8°C. For storage at -20 °C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Rabbit
Species Reactivity:
Goat IgG heavy and light chains (H&L)
Expected Species:
Goat IgG Heavy and Light chains (H&L)
Immunogen:
purified goat IgG, whole molecule
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to non-immunoglobulin goat serum proteins based in immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 10 % (w/v) BSA, Protease/IgG free0,1 % (v/v) of Kathon CG is used as preservative
Goat anti-chicken IgY is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to hen IgY immunoglobulin in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Chicken IgY heavy and light chains (H&L)
Expected Species:
Chicken IgY Heavy and Light chains (H&L)
Immunogen:
purified chicken IgY, whole molecule
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Concentration: 1.50 mg/ml (E 1% at 280 nm = 13.0)Antibody concentration is 1.14 mg/ml and it has been afinity purified on solid phase hen IgY (H&L)Antibody is affinity purified using solid phase Chicken IgG/Y.AP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP or ALP (Alkaline phosphatase) which binds to all rabbit immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid, clear, colorless.
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Rabbit IgG heavy and light chains (H&L)
Immunogen:
Purified Rabbit IgG, whole molecule,
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Based upon IEP, this antibody binds to: heavy chains on rabbit IgGlight chains on all rabbit immunoglobulinsNo reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Loudya et al. (2021) Cellular and transcriptomic analyses reveal two-staged chloroplast biogenesis underpinning photosynthesis build-up in the wheat leaf. Genome Biol. 2021 May 11;22(1):151. doi: 10.1186/s13059-021-02366-3. PMID: 33975629; PMCID: PMC8111775.Bapatla et al. (2021). Modulation of Photorespiratory Enzymes by Oxidative and Photo-Oxidative Stress Induced by Menadione in Leaves of Pea (Pisum sativum). Plants 10, no. 5: 987. https://doi.org/10.3390/plants10050987Szyma ?ska et al. (2019). SNF1-Related Protein Kinases SnRK2.4 and SnRK2.10 Modulate ROS Homeostasis in Plant Response to Salt Stress. Int J Mol Sci. 2019 Jan 2;20(1). pii: E143. doi: 10.3390/ijms20010143.Rozp ?dek et al. (2018). Acclimation of the photosynthetic apparatus and alterations in sugar metabolism in response to inoculation with endophytic fungi. Plant Cell Environ. 2018 Dec 5. doi: 10.1111/pce.13485.Borovik and Grabelnych (2018). Mitochondrial alternative cyanide-resistant oxidase is involved in an increase of heat stress tolerance in spring wheat. J Plant Physiol. 2018 Dec;231:310-317. doi: 10.1016/j.jplph.2018.10.007.
Special application note:
Concentration: 1.5mg/ml.Antibody has been affinity purified on solid phase rabbit IgG (H&L).AP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative
Goat anti-rabbit IgG is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to all rabbit immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store liquid material at 2-8°Cup to 6 months.
Host Animal:
Goat
Species Reactivity:
Rabbit IgG heavy and light chains (H&L)
Expected Species:
Rabbit IgG Heavy and Light chains (H&L)
Immunogen:
Purified Rabbit IgG, whole molecule
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Hanschen et al. (2018). Differences in the enzymatic hydrolysis of glucosinolates increase the defense metabolite diversity in 19 Arabidopsis thaliana accessions. Plant Physiol Biochem. 2018 Mar;124:126-135. doi: 10.1016/j.plaphy.2018.01.009.Giovanardi et al. (2018). In pea stipules a functional photosynthetic electron flow occurs despite a reduced dynamicity of LHCII association with photosystems. Biochim Biophys Acta. 2018 May 24. pii: S0005-2728(18)30129-4. doi: 10.1016/j.bbabio.2018.05.013.Krasuska et al. (2015). Switch from heterotrophy to autotrophy of apple cotyledons depends on NO signal. Planta. 2015 Jul 18.
Special application note:
Antibody has been affinity purified on solid phase rabbit IgG (H&L)AP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody has been afinity purified on solid phase rabbit IgG (H&L)Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. Contains 0.05 % (w/v) sodium azide as preservative.The linker length is 22.4 angstroms.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody concentration is 3.86 mg/ml and it has been afinity purified on solid phase hen IgY (H&L)biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. It contains 0.05 % sodium azide as preservative
No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody concentration is 3.85 mg/ml and it has been afinity purified on solid phase sheep IgG (H&L)biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. It contains 0.05 % sodium azide as preservative
AKING1 is a member of SnRK1/SNF1/AMPK family in plants. It is a regulatory subunit of the putative trimeric SNF1-related protein kinase (SnRK) comples which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana AKING1UniProt: Q8LBB2, TAIR: At3g48530
Emanuelle et al. (2015). SnRK1 from Arabidopsis thaliana is an atypical AMPK. Plant J. 2015 Mar 3. doi: 10.1111/tpj.12813.Ramon et al. (2013). The hybrid Four-CBS-Domain KINβγ-subunit functions as the canonical γsubunit of the plant energy sensor SnRK1. Plant j. April 1.
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). Alternative name: AtBP2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4 C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Piea sitchensis, Populus trichocarpa, Spinacia oleracea, Zea mays Species of your interest not listed? Contact us
Protein or membrane sample should be treated at 70 C for 10 min before loading on the gel
Application Details:
1 : 50-1 : 1000 (IF), 1 : 2000 (WB)
Purity:
Immunogen affinity purified total IgY. in PBS pH 7.4.
Molecular Weight:
73,5 | 80 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Bennett et al. (2014). Plasma Membrane-Targeted PIN Proteins Drive Shoot Development in a Moss. Curr Biol. 2014 Dec 1;24(23):2776-85. doi: 10.1016/j.cub.2014.09.054. Epub 2014 Nov 13.
Special application note:
Antibody solution contains 0,02% sodium azide as preservative
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). Alternative name: AtBP2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Picea sitchensis, Populus trichocarpa, Physcomitrium patens, Spinacia oleracea, Zea maysSpecies of your interest not listed? Contact us
Protein or membrane sample should be treated at 70 C for 10 min before loading on the gel.Antibody has a reduced reactivity to monocots in western blot.
Application Details:
1 : 2000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
73,5 | 80 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Narusaka et al (2016). Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1. Sci Rep. 2016 Jan 11;6:18702. doi: 10.1038/srep18702.
AGO4 (Argonaute 4) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tube.
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca. 40 kDa.Binds endogenous siRNAs, preference for 24nt siRNAs with 5' A.Note that the AGO4 antibody reacts with the NEB prestained protein marker.
Application Details:
1 : 100 (ICC), 5 g of antibody per 1 gram of a fresh tissue (IP),1 : 2000-1 : 5000 (WB)
Clavel et al. (2021) Atypical molecular features of RNA silencing against the phloem-restricted polerovirus TuYV. Nucleic Acids Res. 2021 Nov 8;49(19):11274-11293. doi: 10.1093/nar/gkab802. PMID: 34614168; PMCID: PMC8565345.Oliver & Martinez. (2021) Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis. Plant Reprod. 2021 Nov 23. doi: 10.1007/s00497-021-00434-z. Epub ahead of print. PMID: 34812935.Niedojad?o et al. (2020). Dynamic distribution of ARGONAUTE1 (AGO1) and ARGONAUTE4 (AGO4) in Hyacinthus orientalis L. pollen grains and pollen tubes growing in vitro. Protoplasma. 2020 Jan 8. doi: 10.1007/s00709-019-01463-2.Sprunck et al. (2019). Elucidating small RNA pathways in Arabidopsis thaliana egg cells. http://dx.doi.org/10.1101/525956Yang et al. (2017). The developmental regulator PKL is required to maintain correct DNA methylation patterns at RNA-directed DNA methylation loci. Genome Biol. 2017 May 31;18(1):103. doi: 10.1186/s13059-017-1226-y.
Goat anti-rat IgG is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store non-diluted antibody at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Rat IgG heavy and light chains (H&L) of all Rat immunoglobulins
Expected Species:
Rat IgG Heavy and Light chains (H&L) of all Rat immunoglobulins
Immunogen:
Purified Rat IgG, whole molecule
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to non-immunoglobulin rat serum, Antibody has been absorbed against mouse IgG and no reactivity is observed to human or mouse IgG based on immunoelectrophoresis
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative.
Goat anti-rat IgG is a secondary antibody conjugated to ALP (alkaline phosphatase) which binds to all rabbit immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Rat IgG heavy and light chains (H&L)
Expected Species:
Rat IgG Heavy and Light chains (H&L)
Immunogen:
Purified Rat IgG, whole molecule
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to non-immunoglobulin rat serum proteins based in immunoelectrophoresis, No reacticity to mouse and human serum proteins based on immunoelectrophoresis
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Goat anti-Chicken IgY (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Chicken IgY (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Chicken IgYDyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on chicken IgG (IgY), light chains on all chicken immunoglobulinsNo reactivity is observed to: non-immunoglobulin chicken serum proteins
Rabbit anti-goat IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Goat IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Goat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with:, heavy (γ) chains on goat IgG, light chains on all goat immunoglobulinsNo reactivity is observed to: non-immunoglobulin goat serum proteins, IgG from human, mouse, or rat
Rabbit anti-mouse IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all mouse immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Rabbit
Species Reactivity:
Mouse IgG heavy and light chains (H&L)
Expected Species:
Mouse IgG Heavy and Light chains (H&L)
Immunogen:
Purified Mouse IgG, whole molecule
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Vitale et al. (2021) Light Spectral Composition Influences Structural and Eco-Physiological Traits of Solanum lycopersicum L. cv. 'Microtom' in Response to High-LET Ionizing Radiation. Plants (Basel). 2021 Aug 23;10(8):1752. doi: 10.3390/plants10081752. PMID: 34451797; PMCID: PMC8399554.Bui et al. (2020). Differential submergence tolerance between juvenile and adult Arabidopsis plants involves the ANAC017 transcription factor. doi.org/10.1101/2020.02.12.945923 BioRxiv.Koch et al. (2019). Heat stress directly impairs gut integrity and recruits distinct immune cell populations into the bovine intestine. Proc Natl Acad Sci U S A. 2019 May 21;116(21):10333-10338. doi: 10.1073/pnas.1820130116.
Special application note:
Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative.
Rabbit anti-mouse IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all mouse immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized material at 2-8°C. For storage at -20 °C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Rabbit
Species Reactivity:
Mouse IgG heavy and light chains (H&L)
Expected Species:
Mouse IgG Heavy and Light chains (H&L)
Immunogen:
Purified Mouse IgG, whole molecule
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative.
Donkey anti-sheep IgG is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to all donkey immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Donkey
Species Reactivity:
Sheep IgG heavy and light chains (H&L)
Expected Species:
Sheep IgG Heavy and Light chains (H&L)
Immunogen:
Purified sheep IgG, whole molecule
Applications:
ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to non-immunoglobulin sheep serum proteins based in immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
APL conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Rabbit anti-mouse IgG (H&L) is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all mouse immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid, clear, colorless.
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Rabbit
Species Reactivity:
Mouse IgG heavy and light chains (H&L)
Immunogen:
Purified Mouse IgG, whole molecule,
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB)
Based on IEP, this antibody binds to: heavy chains on mouse IgGlight chains on all mouse immunoglobulinsNo reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.Concentration: 1.50 mg/ml (E 1% at 280 nm = 13.0)
Rabbit anti-goat IgG is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all goat immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Rabbit
Species Reactivity:
Goat IgG heavy and light chains (H&L)
Expected Species:
Goat IgG Heavy and Light chains (H&L)
Immunogen:
Purified Goat IgG, whole molecule
Applications:
ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to non-immunoglobulin goat serum proteins based in immunoelectrophoresis. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Donkey anti-Sheep IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Sheep IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Sheep IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG, light chains on all sheep immunoglobulinsNo reactivity is observed to: non-immunoglobulin sheep serum proteins
Goat anti-mouse IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Mouse IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase mouse IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins
Goat anti-rabbit IgG, DyLight 488 Conjugate is a secondary antibody conjugated to DyLight 488, which binds to all rabbit IgGs in immunological assays.DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis. Purity of this antibody is > 95% based on SDS-PAGE.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Kucko et al. (2022) The acceleration of yellow lupine flower abscission by jasmonates is accompanied by lipid-related events in abscission zone cells, Plant Science, Volume 316, 2022,111173, ISSN 0168-9452, https://doi.org/10.1016/j.plantsci.2021.111173. Namyslov et al. (2020). Exodermis and Endodermis Respond to Nutrient Deficiency in Nutrient-Specific and Localized Manner. Plants (Basel). 2020 Feb 6;9(2). pii: E201. doi: 10.3390/plants9020201. (immunolocalization) Fizesan et al. (2018). Responsiveness assessment of a 3D tetra-culture alveolar model exposed to diesel exhaust particulate matter. Toxicol In Vitro. 2018 Aug 3;53:67-79. doi: 10.1016/j.tiv.2018.07.019.Liu et al. (2016). Fold formation at the compartment boundary of Drosophila wing requires Yki signaling to suppress JNK dependent apoptosis. Sci Rep. 2016 Nov 29;6:38003. doi: 10.1038/srep38003.Wang et al. (2016). Complementary expression of optomotor-blind and the Iroquois complex promotes fold formation to separate wing notum and hinge territories. Dev Biol. 2016 Aug 1;416(1):225-34. doi: 10.1016/j.ydbio.2016.05.020. Epub 2016 May 19
Special application note:
Concentration: 1.0mg/mlConjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight 488 has a maximum absorbance at 493 nm; Emax = 518 nm.
Goat anti-rabbit IgG (H&L), DyLight 488 Conjugated, min. cross-reactivity to bovine, human, mouse IgG or serum proteins is a secondary antibody conjugated to DyLight 488, which binds to Rabbit IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG, light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins, IgG from human or mouse serum proteins from bovine, human or mouse
Donkey anti-Sheep IgG (H&L), DyLight 488 Conjugated, min. cross-reactivity to human or rabbit IgG is a secondary antibody conjugated to DyLight 488, which binds to Sheep IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Sheep IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG, light chains on all sheep immunoglobulinsNo reactivity is observed to: non-immunoglobulin sheep serum proteins, IgG from human or rabbit
Goat anti-mouse IgG (H&L), DyLight 488 Conjugated, min. cross-reactivity to human IgG or serum proteins is a secondary antibody conjugated to DyLight 488, which binds to Mouse IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase mouse IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Wang et al. (2016). Complementary expression of optomotor-blind and the Iroquois complex promotes fold formation to separate wing notum and hinge territories. Dev Biol. 2016 Aug 1;416(1):225-34. doi: 10.1016/j.ydbio.2016.05.020. Epub 2016 May 19Liu et al. (2016). Fold formation at the compartment boundary of Drosophila wing requires Yki signaling to suppress JNK dependent apoptosis. Sci Rep. 2016 Nov 29;6:38003. doi: 10.1038/srep38003.
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins, human IgG or serum proteins
Goat anti-Rat IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Rat IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG, light chains on all rat immunoglobulinsNo reactivity is observed to: non-immunoglobulin rat serum proteins
Goat anti-Rat IgG (H&L), DyLight 488 Conjugated, min. cross-reactivity to human, mouse lgG is a secondary antibody conjugated to DyLight 488, which binds to Rat IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG, light chains on all rat immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins, IgG from human or mouse
S-nitrosoglutathione reductase (GSNOR) is a cytoplasm localized enzyme which plays a key role in formaldehyde detoxification and is down regulated by wounding and activated by salicylic acid (SA).Alternative protein names: Alcohol dehydrogenase class-3, Alcohol dehydrogenase class-III, FALDH, FDH formaldehyde dehydrogenase, alcohol dehydrogenase III, HOT5, S-(hydroxymethyl)glutathione dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GSH-FDH
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Brassica napus, Oryza sativa, Pisum sativum, Populus balsamifera, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
Overexpressed, full length GSNOR derived from Arabidopsis thaliana Q96533 , At5g43940
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Zhang et al (2021) Induction of S-nitrosoglutathione reductase protects root growth from ammonium toxicity by regulating potassium homeostasis in Arabidopsis and rice. J Exp Bot. 2021 Mar 27:erab140. doi: 10.1093/jxb/erab140. Epub ahead of print. PMID: 33772588.Borb ly et al. (2021) The Effect of Foliar Selenium (Se) Treatment on Growth, Photosynthesis, and Oxidative-Nitrosative Signalling of Stevia rebaudiana Leaves. Antioxidants (Basel). Jan 8;10(1):E72. doi: 10.3390/antiox10010072. PMID: 33429850.Labudda et al. (2020). Cyst Nematode Infection Elicits Alteration in the Level of Reactive Nitrogen Species, Protein S-Nitrosylation and Nitration, and Nitrosoglutathione Reductase in Arabidopsis thaliana. Antioxidants (Basel) . 2020 Aug 26;9(9):E795.doi: 10.3390/antiox9090795. Moln r et al. (2020). Nitro-oxidative Signalling Induced by Chemically Synthetized Zinc Oxide Nanoparticles (ZnO NPs) in Brassica Species. Chemosphere, 251, 126419 Zhang et al. (2020). Glutathione-dependent denitrosation of GSNOR1 promotes oxidative signaling downstream of H2 O2. Plant Cell Environ. 2020 Jan 28. doi: 10.1111/pce.13727.
Special application note:
Antibody is easily detecting GSNOR in a load per well of 5 g of total Arabidopsis thaliana cell extract
Ricin is a protein found in castor bean (Ricinus communis) acting as protein synthesis inhibitor. It is a toxin involved in plant defence. A chain of ricin can inactivate few thousands of ribosomes per minute. B chain facilitates the entry of A chain into the cell.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store at -70 C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. , For storage at 4°Csodium azide can be added
Detected epitope is located on the surface of RTA subunit not accssible in intact ricin molecule, This antibody does not cross-react to whole ricin molecule
Application Details:
The optimal working dilution should be determined by the investigator, for specific application
Conjugation:
IgG1
Isotype:
IgG1
Purity:
Purified IgG in PBS pH 7.4 with mannitol.
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
29.8 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibodies have been purified by affinity chromatography on Protein G from tissue culture supernatant and lyophilized from solution containing: mannitol 1%, dextran 1%, NaCl 100mM, sodium phosphate 10 mM, pH 7.5. If required, mannitol can be removed either by dialysis or by chromatography using mini columns Sephadex G50 fine. The concentration can be measured by absorbance at 280 nm (1.35 for 1 mg/ml) or by Bradford methodRicin detection limit is 5 ng/ml, signal-to-noise ratio is 15-50 up to 100 ng/mlEpitope recognized by this antibody is located on the surface of RTA subunit and not accessible in intact ricin molecule. Suggested protocol can be found here.
Ricin is a protein found in castor bean (Ricinus communis) acting as protein synthesis inhibitor. It is a toxin involved in plant defence. A chain of ricin can inactivate few thousands of ribosomes per minute. B chain facilitates the entry of A chain into the cell.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store at -70 C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. , For storage at 4°Csodium azide can be added
The optimal working dilution should be determined by the investigator, for specific application
Conjugation:
IgG1
Isotype:
IgG1
Purity:
Purified IgG in PBS pH 7.4 with mannitol.
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
29,8 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibodies have been purified by affinity chromatography on Protein G from tissue culture supernatant.Ricin detection limit is 5 ng/ml, signal to noise ratio is 15-50 up to 100 ng/mlAs detection antibody in sandwich-type ELISA, antibody, AS09 649, which is recongnizing a complimentary epitope is recommended. This product is conjugated. Suggested protocol can be found here.
Donkey anti-sheep IgG (H&L) is a non-conjugated secondary antibody which binds to all sheep IgGs in immunological assays. Antibodies are affinity purified using solid phase sheep IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified donkey IgG.
Special application note:
This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgG Fc, is an adsorbed F(ab)'2 fragment of secondary antibody conjugated to Rhodamine (TRITC) which binds to human IgG Fc part. Antibodies are conjugated to tetramethylrhodamine-5-isothiocyanate (TRITC). Amax = 550 nm, Emax = 570 nm. Purity is >90% based on SDS-PAGE. Small amounts of intact IgG may be present.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains on human immunoglobulins or to non-immunoglobulin human serum proteins based on immunoelectrophoresis.No reactivity is observed to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgG (H&L), is an adsorbed F(ab)'2 fragment of secondary antibody conjugated to FITC (fluorescein-5-isothiocyanate). Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Rabbit anti-goat IgG Fc is a secondary antibody to goat Fc part conjugated to HRP. Antibodies are affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on goat IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed to the light chains or to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Chicken anti-mouse IgG (H&L) is an adsorbed secondary antibody which binds to all mouse IgGs,Rhodamine (TRITC) conjugated. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator, (ICC), immunohistochemistry ( IHC) (Flow cyt)
Purity:
Immunogen affinity purified chicken IgY.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis. Minimum cross-reactivity is observed with human or rabbit IgG or serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
Goat anti-human IgA + IgG + IgM is a secondary antibody, which binds to human IgA, IgG and IgM and is FITC (fluorescein-5-isothiocyanate) conjugated. Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase human IgG + IgA + IgM.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy and light chains on human IgA, IgG and IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-rat IgG (H&L), ALP (alkaline phosphatase) is a secondary antibody which binds to all rat IgGs in immunological assays. Antibodies are affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free and , 0.05 % (w/v) sodium azide as preservative.
Donkey anti-rabbit IgG (H&L) is a secondary antibody, HRP conjugated, which binds to all rabbit IgGs in immunological assays. Antibody is specially adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,mouse,rat,sheep IgG. Antibodies are affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Petersen and Andersen (2014). Simultaneous isolation of mRNA and native protein from minute samples of cells. Biotechniques. 2014 May 1;56(5):229-37. doi: 10.2144/000114165. eCollection 2014.
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Goat anti-human IgM, chain, is a F(ab)'2 fragment, of HRP conjugated secondary antibody which binds to human IgM, chain in immunological assays. Antibodies are affinity purified using solid phase human IgM.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on human IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed with IgG, F(ab)'2 fragment based on immunoelectrophoresis. No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Goat anti-rabbit IgG (H&L), F(ab)'2 fragment of FITC (fluorescein-5-isothiocyanate) conjugated secondary antibody which binds to rabbit IgG in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody purity is >90% based on SDS-PAGE and is affinity purified on solid phase rabbit IgG. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgE (ε chain), is a Rhodamine conjugated (TRITC-tetramethylrhodamine-5-isothiocyanate) secondary antibody which binds to human IgE in immunological assays. (TRITC) has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against bovine,mouse,rabbit serum and affinity purified using solid phase human IgE.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the ε chains on human IgE based on immunoelectrophoresis. Minimum cross-reactivity is observed to the light chains or non-IgE human serum proteins based on immunoelectrophoresis.Minimum cross-reactivity is observed against serum proteins from bovine, mouse or rabbit based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-rabbit IgG (H&L), HRP conjugated is a secondary antibody which binds to all rabbit IgGs in immunolgical assays. Antibodies are affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins, mouse IgG or mouse serum by immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Goat anti-human IgG (H&L) is a FITC (fluorescein-5-isothiocyanate) conjugated secondary antibody F(ab)'2 fragment, which binds to all human IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody is adsorbed to bovine,mouse,rabbit serum and its purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human, mouse, rabbit or bovine serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-rat IgG (H&L) is ALP (alkaline phosphatase) conjugated secondary antibody which reacts with all rat IgGs. Antibody is specially adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,mouse,rabbit and sheep IgG and affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, or sheep based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free and , 0.05 % (w/v) sodium azide as preservative.
Goat anti-rabbit IgG (H&L) is a F(ab)'2 fragment of ALP (alkaline phosphatase) conjugated secondary antibody which binds to all rabbit IgGs in immunological assays. Antibody is adsorbed to bovine,human, mouse IgG/serum and its purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with:heavy chains on rabbit IgG light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from bovine, goat, human, mouse or rat.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free and , 0.05 % (w/v) sodium azide as preservative.
Goat anti-rabbit IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated F(ab)'2 fragment is a secondary antibody which binds to all rabbit IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG and is affinity purified on solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Feng et al. (2020). Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway by inhibiting cytosolic ferritin and inducing chromatin autophagy. BMC Cancer. 2020 Jul 16;20(1):665.doi: 10.1186/s12885-020-07133-4.
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-rabbit IgG (H&L) is a Rhodamine (TRITC - tetramethylrhodamine-5-isothiocyanate) conjugated secondary antibody which binds to all rabbit IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody is adsorbed to bovine,chicken,goat, guinea pig, hamster, horse,human,mouse,rat,sheep IgG and affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator, (ICC), immunohistochemistry ( IHC) (Flow cyt)
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgG Fc part, biotin conjugated is a secondary antibody which binds to mous IgG Fc part in immunological assays. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
Donkey anti-goat IgG (H&L), in a non-conjugated secondary antibody which binds to all goat IgGs in immunological assays. Antibody is adsorbed against human,mouse,rabbit,rat IgG and is affinity purified using solid phase goat IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse, rabbit, or rat based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7 and 0.05 % (w/v) sodium azide as preservative. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-rat IgG (H&L), affinity purified, is unconjugated secondary antibody which binds to all rat IgGs in immunological assays. Antibody is adsorbed against human,mouse IgG and affinity purified using solid phase rat IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified chicken IgY.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis. Highly cross absorbed against mouse IgG.Minimum cross-reactivity is observed to non- immunoglobulin rat serum proteins based on immunoelectrophoresis. Minimum cross-reactivity is observed to human or mouse IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7and 0.05 % (w/v) sodium azide as preservative.
Goat anti-rat IgG (H&L), F(ab)'2 fragment, biotin conjugated, is F(ab)'2 fragment, of a biotinylated secondary antibody which binds to all rat IgGs in immunological assays. Antibody is adsorbed against human,mouse IgG and affinity purified on solid phase rat IgG. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or to human or mouse IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Donkey anti-sheep IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to all sheep IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody is adsorbed against human,mouse,rabbit IgG and affinity purified using solid phase sheep IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins and human,mouse,rabbit IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Chicken anti-goat IgG (H&L) is a biotin conjugated secondary antibody which binds to all goat IgGs in immunological assays. Antibody is adsorbed to human, mouse, rabbit IgG/serum and affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgY.
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-mouse IgG (H&L), ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to all mouse IgGs in immunological assays. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilution prior to use and then discard. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free and , 0.05 % (w/v) sodium azide as preservative.
Chicken anti-rat IgG (H&L), affinity purified, is an unconjugated secondary antibody which binds to rat IgGs in immunological assays. Antibody is adsorbed against to human,rabbit, IgG/serum and affinity purified using solid phase rat IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgY.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins and human,rabbit, IgG/serum based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7 and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgM, ( chain) is a F(ab)'2 fragment, of secondary antibody which binds to human IgM in immunological assays. Antibody is affinity purified and its purity is > 90% based on SDS-PAGE. Solution may contain small amounts of intact IgG.
Goat anti-rabbit IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which bings to all rabbit IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody is adsorbed against bovine,human,mouse IgG/serum and affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins and bovine,human,mouse IgG/serum based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Chicken anti-mouse IgG (H&L),HRP conjugated is a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are affinity purified using solid pahse mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgY.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.2)This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
Chicken anti-goat IgG (H&L) is an affinity purified secondary antibody which binds to all goat IgGs in immunological assays. Antibody is adsorbed to to human,mouse,rabbit IgG/serum and affinity purified using solid phase goat IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgY.
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7 and 0.05 % (w/v) sodium azide as preservative. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-human IgM, chain,HRP conjugated is a secondary antibody which binds to human IgM in immunological assays. Antibodies affinity purified using solid phase human IgM.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on human IgM based on immunoelectrophoresis. Minimum cross-reactivity is observed with IgG, F(ab)'2 fragment based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
Rabbit anti-mouse IgG (H&L), Rhodamine (TRITC - tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to all mouse IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody is adsorbed against human serum and affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator, (ICC), immunohistochemistry ( IHC) (Flow cyt)
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins, or human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgG + IgA + IgM, HRP conjugated is a F(ab)'2 fragment, of a secondary antibody which binds to human IgG+IgA+IgM in immunological assays. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Wielkoszynski et al. (2018). Novel diagnostic ELISA test for discrimination between infections with Yersinia enterocolitica and Yersinia pseudotuberculosis. Eur J Clin Microbiol Infect Dis. 2018 Dec;37(12):2301-2306. doi: 10.1007/s10096-018-3373-9.
Special application note:
This antibody reacts with the heavy chains on human IgG + IgA + IgM, and the light chains on all human immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
Goat anti-rat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to all rat IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody is adsorbed against human,mouse IgG and affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or to human or mouse IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-rabbit IgG (H&L), F(ab)'2 fragment, is FITC (fluorescein-5-isothiocyanate) conjugated secondary antibody which binds to all rabbit IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody is adsorbed against bovine, human,mouse IgG/serum and its purity is is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-goat IgG (H&L) is an ALP (alkaline phosphatase) conjugated secondary antibody which reacts with all goat IgGs in immunological assays. Antibody is adsorbed to human,mouse,rabbit,rat IgG and affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse, rabbit, or rat based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Rabbit anti-goat IgG (H&L),HRP conjugated is a F(ab)'2 fragment of a secondary antibody which binds to all goat IgGs in immunological assays. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Rabbit
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-goat IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to all goat IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-rabbit IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to all rabbit IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against mouse IgG/serum and affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins, mouse IgG or mouse serum based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-guinea pig IgG (H&L) is a Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated secondary antibody which binds to all guinea pig IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody is adsorbed to bovine,chicken, goat,hamster,horse, human,mouse,rabbit,rat,sheep serum and affinity purified using solid phase guinea pig IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
ImmunogenImmunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on guinea pig IgG and with the light chains on all guinea pig immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin guinea pig serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to all mouse IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against human IgG/serum and affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-guinea pig IgG (H&L) is an affinity purified unconjugated secondary antibody which binds to all guinea pig IgGs in immunological assays. Antibody is adsorbed to bovine, chicken, goat, hamster, horse, human, mouse, rabbit, rat, sheep serum and affinity purified using solid phase guinea pig IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on guinea pig IgG and with the light chains on all guinea pig immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin guinea pig serum proteins or serum from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, or sheep based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Goat anti-rabbit IgG (H&L) is a biotin conjugated secondary antibody which binds to all rabbit IgGs in immunological assays. Antibody is adsorbed against bovine,goat,human,mouse,rat IgG and affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis. Minimum cross-reactivity is observed to IgG from bovine, goat, human, mouse or rat based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Goat anti-human IgG Fc, Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated,is a secondary antibody which binds to human IgG constant part in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against human IgA+IgM and affinity purified using solid phase human IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains on human immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to human IgM or IgA based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Rabbit anti-goat IgG (H&L) is a secondary antibody F(ab)'2 fragment, which binds to all goat IgGs in immunological assays and is FITC (fluorescein-5-isothiocyanate) conjugated. Antibody is also specially adsorbed against human,mouse and rat IgG. FITC Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody purity is > 90% based on SDS-PAGE, may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse or rat based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-rabbit IgG (H&L), Rhodamine (TRITC - tetramethylrhodamine-5-isothiocyanate) conjugated, is a F(ab)'2 fragment of a secondary antibody which binds to all rabbit IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody is adsorbed against bovine,human,mouse IgG/serum. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins and bovine,human,mouse IgG/serum based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Rabbit anti-goat IgG (H&L), affinity purified, is an unconjugated secondary antibody which binds to all goat IgGs in immunological assays. Antibody is adsorbed against human,mouse,rat IgG and affinity purified using solid phase goat IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse or rat based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 0.05 % (w/v) sodium azide as preservative. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.Expiration date: November 6, 2017
Donkey anti-goat IgG (H&L), affinity purified is an unconjugated secondary antibody which binds goat IgGs in immunological assays. Antibodies are affinity purified using solid phase goat IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Chicken anti-rabbit IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to all rabbit IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgY.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Rabbit anti-goat IgG (H&L) is a HRP conjugated F(ab)'2 fragment of a secondary antibody which reactis with all goat IgGs in immunological assays. Antibody is adsorbed against human,mouse and rat IgG. Its purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse or rat based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-mouse IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibodies which binds to all mouse IgGs in immunological assays. Antibody is adsorbed against human IgG/serum and affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Chicken anti-goat IgG (H&L),HRP conjugated is a secondary antibody which binds to goat IgGs in immunological assays. Antibodies are affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-goat IgG (H&L), HRP conjugated, is a secondary antibody which reacts with all goat IgGs in immunological assays. Antibodies are adsorbed against human,mouse,rabbit,rat IgG and affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse, rabbit, or rat based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-mouse IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a F(ab)'2 fragment, of a secondary antibody which binds to mouse IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody is adsorbed against human IgG/serum. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-rat IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are adsorbed against human,mouse IgG and affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilution prior to use and then discard. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or to human or mouse IgG based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgA (alpha chain), FITC (fluorescein-5-isothiocyanate) conjugated, F(ab)'2 fragment of a secondary antibody which binds to human IgA in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the alpha chains on human IgA based on immunoelectrophoresis.Minimum cross-reactivity is observed to the light chains or non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Rabbit anti-goat IgG (H&L) is a F(ab)'2 fragment, of unconjugated, affinity purified secondary antibody which binds all goat IgGs in immunological assays. Antibody is adsorbed against human,mouse,rat IgG and its purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse or rat based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-goat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to all goat IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-Rabbit IgG (H&L), DyLight 488 Conjugated, min. cross-reactivity to human IgG and serum proteins is a secondary antibody conjugated to DyLight 488, which binds to Rabbit IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rabbit IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG, light chains on all rabbit immunoglobulinsNo reactivity is observed to: non-immunoglobulin rabbit serum proteins, mouse IgG or serum proteins
Goat anti-guinea pig IgG (H&L), DyLight 488 Conjugated, min. cross-reactivity to bovine, chicken, goat, hamster, horse, human, mouse, rabbit, rat, sheep Serum is a secondary antibody conjugated to DyLight 488, which binds to Guinea pig IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Guinea Pig IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on guinea pig IgG, light chains on all guinea pig immunoglobulinsNo reactivity is observed to: non-immunoglobulin guinea pig serum proteins, serum proteins from bovine, chicken, goat, hamster, horse, human, mouse, rabbit, rat or sheep
Goat anti-mouse IgG (H&L), biotin conjugated, F(ab)'2 fragment is a secondary antibody which binds to mouse IgGs in immunological assays. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
Donkey anti-mouse IgG (H&L) is an affinity purified using solid phase mouse IgG, secondary antibody which reacts with all mouse IgGs and is adsorbed against bovine, chicken,goat,guinea pig, hamster,horse,human, rabbit,rat and sheep IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Donkey anti-rabbit IgG (H&L) is a biotin conjugated secondary antibody which reacts with all rabbit IgGs in immunological assays. Antibody is adsorbed to chicken,goat,guinea pig,hamster,horse,human,mouse,rat and sheep IgG and affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
Goat anti-human IgG Fc, FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to human IgG Fc constant region in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against bovine,mouse,rabbit serum and affinity purified using solid phase human IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains on human immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin bovine, human, mouse or rabbit serum proteins, or mouse and rabbit IgG.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-rabbit IgG (H&L) is an ALP (alkaline phosphatase) conjugated secondary antibody which binds all rabbit IgGs in immunological assays. Antibody is adsorbed againstbovine,goat,human,mouse,rat IgG and affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Wężowicz et al. (2017). Interactions of arbuscular mycorrhizal and endophytic fungi improve seedling survival and growth in post-mining waste. Mycorrhiza. 2017 Mar 20. doi: 10.1007/s00572-017-0768-x.
Special application note:
This antibody reacts with: heavy chains on rabbit IgG light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from bovine, goat, human, mouse or rat. Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Donkey anti-mouse IgG (H&L) is aHRP conjugated secondary antibody which reacts to all mouse IgGs in immunological assays. Antibody is adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,rabbit,rat,sheep IgG and affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Goat anti-rat IgG (H&L) is a F(ab)'2 fragment, of Rhodamine (TRITC - tetramethylrhodamine-5-isothiocyanate) conjugated secondary antibody which binds to all rat IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody has been adsorbed against human and mouse IgG and its purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from human or mouse based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to human IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase human IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgM ( chain), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a F(ab)'2 fragment of a secondary antibody which binds human IgM in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody is adsorbed against human IgG or IgA and affinity purified using solid phase human IgM.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the mu chains on human IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed with IgG, F(ab)'2 fragment, IgG or IgA based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgA (alpha chain), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to human IgA in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase human IgA.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the alpha chains on human IgA based on immunoelectrophoresis.Minimum cross-reactivity is observed to the light chains or non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-goat IgG (H&L) is a FITC (fluorescein-5-isothiocyanate) conjugated secondary antibody which reacts with all goat IgGs in immunological assays. Antibody has been adsorbed against human,mouse,rabbit,rat IgG and affinity purified using solid phase goat IgG. Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse, rabbit, or rat based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-rat IgG (H&L) is a secondary, biotin conjugated antibody which reacts with all rat IgGs in immunologica assays. Antibody has been dsorbed against to bovine,chicken,goat,guinea pig,hamster,horse,human,mouse,rabbit and sheep IgG and affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
Goat anti-rat IgG (H&L),HRP conjugated, is a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are adsorbed against human,mouse IgG and affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from human or mouse.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
Goat anti-mouse IgG (H&L), biotin conjugated is a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
Goat anti-mouse IgG (H&L), DyLight 488 Conjugated, min. cross-reactivity to bovine, goat, human, rabbit, rat IgG is a secondary antibody conjugated to DyLight 488, which binds to Mouse IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase mouse IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins, IgG from bovine, goat, human, rabbit or rat
Goat anti-human IgA (alpha chain), F(ab)'2 fragment, TRITC -tetramethylrhodamine-5-isothiocyanate conjugated is a secondary antibody which binds to human IgA in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the alpha chains on human IgA based on immunoelectrophoresis.Minimum cross-reactivity is observed to the light chains or non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-sheep IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to sheep IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase sheep IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°CShelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-human IgG Fc, F(ab)'2 fragment, FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to human IgG Fc constant part in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against bovine,mouse,rabbit serum with a purify of >90% based on SDS-PAGE. Small amounts of intact IgG may be present.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains on human immunoglobulins or to non-immunoglobulin human serum proteins based on immunoelectrophoresis. No reactivity is observed to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative. 1% (w/v) BSA, Protease/IgG free.
Rabbit anti-goat IgG Fc, Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to goat IgG Fc constant part in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified on solid phase goat IgG Fc.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on goat IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or immunoglobulin light chains based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Rabbit anti-goat IgG (H&L), Rhodamine (TRITC- tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to goat IgGs in immunological assays. TRITC has Amax = 550 nm Emax = 570 nm. Antibodies are adsorbed against human,mouse,rat IgG and affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse or rat based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-rat IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to all rat IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative. 1% (w/v) BSA, Protease/IgG free.
Chicken anti-mouse IgG (H&L), biotin conjugated, is a secondary antibody which bind to all mouse IgGs in immunological assays. Antibodies are adsorbed against human, rabbit IgG/serum and affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgY.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis. Minimum cross-reactivity is observed with human or rabbit IgG or serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
Goat anti-chicken IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to chicken IgG (called also chicken IgY) in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase chicken IgY (called also chicken IgG).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on chicken IgG and with the light chains on all chicken immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin chicken serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to human IgG in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against bovine,mouse,rabbit serum and affinity purified using solid phase human IgG. (H&L)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human, mouse, rabbit or bovine serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Rabbit anti-goat IgG Fc,HRP conjugated, is a secondary antibody which binds to goat IgG Fc constant part in immunological assays. Antibodies are adsorbed against human serum and affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on goat IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or immunoglobulin light chains based on immunoelectrophoresis.Minimum cross-reactivity is observed with human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Chicken anti-rat IgG (H&L),HRP conjugated, is a secondary antibody which binds to all rat IgGs in immunological assays. Antibodies are adsorbed against to human,rabbit IgG/serum and affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins and human and rabbit IgG/serm based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
Goat anti-rat IgG (H&L), affinity purified, is a secondary antibody which binds to rat IgG in immunological assays. Antibodies are adsorbed against human,mouse IgG Antibodies and affinity purified using solid phase rat IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from human or mouse based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Donkey anti-mouse IgG (H&L), ALP (alkaline phosphatase) conjugated is a secondary antibody which reactis to all mouse IgGs in immunological assays. Antibody is adsorbed against bovine,chicken,goat, guinea pig,hamster,horse,human,rabbit,rat,sheep IgG and affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Chicken anti-goat IgG (H&L), ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to goat IgGs in immunological assays. Antibodies are affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Rabbit anti-mouse IgG (H&L), affinity purified, is a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are adsorbed against human serum and affinity purified using solid phase mouse IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins, or human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Rabbit anti-mouse IgG (H&L),HRP conjugated, is a secondary atnibody which binds to mouse IgGs in immunological assays. Antibodies are adsorbed against human serum and affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins, or human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Donkey anti-rat IgG (H&L),HRP conjugated is a secondary antibody which binds to rat IgGs in immunological assays, except rat Ig1. Antibody is adsorbed against bovine,chicken,goat,guinea pig,hamster, horse,human, mouse,rabbit,sheep IgG and affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis, except rat Ig1.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, or sheep by immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Donkey anti-Goat IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Goat IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Goat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with:, heavy chains on goat IgG, light chains on all goat immunoglobulinsNo reactivity is observed to, non-immunoglobulin goat serum proteinsBSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-guinea pig IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to all guinea pig IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody is adsorbed against bovine,chicken,goat, hamster, horse, human,mouse, rabbit,rat,sheep serum and affinity purified using solid phase guinea pig IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on guinea pig IgG and with the light chains on all guinea pig immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin guinea pig serum proteins and bovine,chicken,goat, hamster, horse, human,mouse, rabbit,rat,sheep serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, are secondary antibodies which bind to all mouse IgGs in immunological assays. (TRITC) has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against goat,human,rabbit,rat IgG and are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins amd goat,human,rabbit,rat IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-rabbit IgG (H&L), affinity purified, is a secondary antibody which binds to all rabbit IgGs in immunological assays. Antibodies are adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,mouse,rat,sheep IgG and affinity purified using solid phase rabbit IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins and bovine,chicken,goat,guinea pig,hamster,horse,human,mouse,rat,sheep IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
This product is a recombinant protein standard, source: Synechocystis strain PCC 6803.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Concentration: after adding 225 l of milliQ water final concentration of the standard is 0.15 pmoles/ lProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:
Standard curve: 3 loads are recommended (2.5 and 10 μl).For most applications a sample load of 0.2μg of chlorophyll will give a IsiA signal in this range.Positive control:a 2μl load per well is optimal for most chemiluminescent detection systems.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:
For reconstitution add 225 l of milliQ water
Molecular Weight:
27 kDa (slightly larger than native protein due to His-tag)
Selected references:
Fraser et al. (2013). Photophysiological and Photosynthetic Complex Changes during Iron Starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942.PLoS ONE 8(3): e59861. doi:10.1371/journal.pone.0059861Ryan-Keogh et al. (2012). Iron deficiency in cyanobacteria causes monomerization of photosystem I trimers and reduces the capacity for state transitions and the effective absorption cross section of photosystem I in vivo. J. of Phycology, 1:145-154.
Special application note:
The IsiA protein standard can be used in combination with anti-IsiA antibodies to quantitate IsiA from a range of cyanobacteria. Global antibodies are raised against highly conserved amino acid sequences in theIsiA protein.Quantitative western blot: detailed method description, video tutorial
Goat anti-rabbit IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to rabbit IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against bovine,goat,human,mouse,rat IgG and affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins and bovine,goat,human,mouse,rat IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgA (alpha chain), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to human IgA in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against bovine,mouse,rabbit serum and affinity purified using solid phase human IgA.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the alpha chains on human IgA based on immunoelectrophoresis.Minimum cross-reactivity is observed to the light chains or non-IgA human serum proteins and bovine,mouse,rabbit serum based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to human IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase human IgG. (H&L)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Chicken anti-mouse IgG (H&L), ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgM ( chain) HRP conjugated, is a secondary antibody which binds to human IgM in immunological assays. Antibodies are adsorbed against human IgA or IgG and affinity purified using solid phase human IgM.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with:heavy ( ) chains on human IgM based on immunoelectrophoresis,Minimum cross-reactivity is observed to: non-immunoglobulin human serum protein, light chains on all human immunoglobulins and human IgA and IgG based on immunoelectrophoresis. Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Donkey anti-rabbit IgG (H&L), ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to all rabbit IgGs in immunological assays. Antibody is adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,mouse,rat,sheep IgG and affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins and bovine,chicken,goat,guinea pig,hamster,horse,human,mouse,rat,sheep IgG, based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Goat anti-rat IgG (H&L), affinity purified is a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are affinity purified using solid phase rat IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Chicken anti-goat IgG (H&L), affinity purified is a secondary antibody which binds to goat IgGs in immunological assays. Antibodies are affinity purified using solid phase goat IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgY.
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-sheep IgG (H&L), Rhodamine (TRITC-tetramethyl rhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to sheep IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against and human,mouse,rabbit IgG and affinity purified using solid phase sheep IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins and human,mouse,rabbit IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, ,1% (w/v) BSA, Protease/IgG free, 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-human IgM ( chain), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to human IgM in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against human IgA or IgG and affinity purified.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with: heavy ( ) chains on human IgM based on immunoelectrophoresis,Minimum cross-reactivity is observed to: non-immunoglobulin human serum proteins IgG, light chains on all human immunoglobulins and human IgA or IgG.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-rat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to all rat IgGs in immunological assays. FITC Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Chicken anti-goat IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to goat IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgY.
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-rat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated are secondary antibodies which bind to rat IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. luor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative. 1% (w/v) BSA, Protease/IgG free.
Goat anti-mouse IgG (H&L), ALP (alkaline phosphatase) conjugated is a F(ab)'2 fragment of a secondary antibody which binds to mouse IgG in immunological assays. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgA (alpha chain), Rhodamine (TRITC- tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to human IgA in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase human IgA.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the alpha chains on human IgA based on immunoelectrophoresis.Minimum cross-reactivity is observed to the light chains or non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgG + IgA + IgM, affinity purified is a secondary antibody which binds human IgG and IgA and IgM in immunological assays. Antibodies are affinity purified using solid phase human IgG. + IgA + IgM.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy and light chains on human IgG + IgA + IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Chicken anti-rabbit IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to rabbit IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Antibodies are adsorbed against human,mouse IgG and affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins and human and mouse IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Rabbit anti-goat IgG Fc, biotin conjugated, is a secondary antibody which binds to goat IgG Fc part in immunological assays. Antibody is adsorbed against human serum and affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on goat IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains on goat immunoglobulins or to non-immunoglobulin goat serum proteins based on immunoelectrophoresis. Minimum cross-reactivity is observed with human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-rabbit IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to rabbbit IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against mouse IgG/serum and affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins, mouse IgG or mouse serum based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-rat IgG (H&L), HRP conjugated, is a F(ab)'2 fragment of a secondary antibody which binds to rat IgGs in immunological assasys. Antibodies are adsorbed against human,mouse IgG and affinity purified. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from human or mouse.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Goat anti-rabbit IgG (H&L), HRP conjugated, is a F(ab)'2 fragment of a secondary antibody which binds to rabbit IgGs in immunological assays. Antibodies are adsorbed against bovine,human,mouse IgG/serum and affinity purified. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Banday and Lajon (2017). Elevated systemic glutamic acid level in the non-obese diabetic mouse is Idd linked and induces beta cell apoptosis. Immunology. 2017 Feb;150(2):162-171. doi: 10.1111/imm
Special application note:
This antibody reacts with:heavy chains on rabbit IgG light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to: non-immunoglobulin rabbit serum proteins serum proteins from bovine, human or mouse IgG from human or mouse.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Donkey anti-sheep IgG (H&L), biotin conjugated, is a secondary antibody which binds to sheep IgGs in immunological assays. Antibodies are adsorbed against human,rabbit IgG and affinity purified using solid phase sheep IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins and human and rabbit IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-sheep IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to sheep IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against human,rabbit IgG and affinity purified using solid phase sheep IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins and human,rabbit IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, ,1% (w/v) BSA, Protease/IgG free, 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Rabbit anti-mouse IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to mouse IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgE (epsilon chain), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to human IgE in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase human IgE.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the epsilon chains on human IgE based on immunoelectrophoresis.Minimum cross-reactivity is observed to the light chains or non-IgE human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgA + IgG + IgM, Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to human IgG and IgA and IgM in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase human IgG. + IgA + IgM.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy and light chains on human IgA, IgG and IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-rat IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to rat IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against human,mouse IgG and affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or to human or mouse IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-rabbit IgG (H&L), biotin conjugated, is a F(ab)'2 fragment of a secondary antibody which binds to all rabbit IgGs in immunological assays. Antibodies are adsorbed against bovine,human,mouse IgG/serum and affinity purified. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis. Minimum cross-reactivity was observed with bovine, human and mouse serum or human and mouse IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
Rabbit anti-goat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to goat IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like donkey serum or commercial formulations which are free from bovine IgG.
Goat anti-mouse IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to mouse IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against bovine,human,rabbit,rat IgG and affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins and bovine,human,rabbit,rat IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a F(ab)'2 fragment,of a secondary antibody which binds to human IgGs in immunological assasy. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against bovine,mouse, rabbit serum and affinity purified. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human, mouse, rabbit or bovine serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgG (H&L), ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilution prior to use and then discard. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Donkey anti-sheep IgG (H&L),HRP conjugated, is a secondary antibody which binds to sheep IgGs in immunological assays. Antibodies are adsorbed against human,rabbit IgG and affinity purified using solid phase sheep IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins based on immunoelectrophoresis. Minimum cross-reactivity is observed with IgG from human or rabbit based on immunoelectrophoresis.Concentration: 1.0 mg/mlAntibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Chicken anti-goat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to goat IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-mouse IgM, ( chain) affinity purified is a secondary antibody which binds to mouse IgM in immunological assay. Antibodies are affinity purified using solid phase mouse IgM.
The optimal working dilution should be determined by the investigator, Antibody is suitbale for: ELISA, ICC, IHC, WB
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the mu chain on mouse IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis and and has limited reaction with purified mouse IgG based on ELISA.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 0.05 % (w/v) sodium azide as preservative. The purity > 95 % based on SD-PAGE.
Goat anti-mouse IgG (H&L), is a F(ab)'2 fragment, of affinity purified secondary antibody which binds to mouse IgGs in immunological assays. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Donkey anti-Rabbit IgG (H&L), DyLight 488 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG is a secondary antibody conjugated to DyLight 488, which binds to Rabbit IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rabbit IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG, light chains on all rabbit immunoglobulinsNo reactivity is observed to: non-immunoglobulin rabbit serum proteins, IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep
Chicken anti-mouse IgG (H&L),HRP conjugated, is a secondary antibody which binds to mouse IgGs in immunological asssyas. Antibodies are adsorbed against human,rabbit IgG/serum and affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgY.
Reconstitution:
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins and human and rabbit IgG/serum based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Donkey anti-rabbit IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which reacts to all rabbits IgGs. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody has been adsorbed to bovine,chicken,goat,guinea pig,hamster,horse,human,mouse,rat,sheep IgG and affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins and bovine,chicken,goat,guinea pig,hamster,horse, human,mouse,rat,sheep IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-sheep IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to sheep IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase sheep IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-Bovine IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Bovine IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Bovine IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on bovine IgG, light chains on all bovine immunoglobulinsNo reactivity is observed to: non-immunoglobulin bovine serum proteins
Goat anti-human IgM ( chain), FITC (fluorescein-5-isothiocyanate) conjugated are secondary antibodies which bind to human IgM in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies affinity purified using solid phase human IgM.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the mu chains on human IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed with non-immunoglobulin human serum proteins and light chains on all human immunoglobulins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-rabbit IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated are secondary antibodies which bind to rabbit IgG in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Kolbert et al. (2018). Nitro-oxidative stress correlates with Se tolerance of Astragalus species. Plant Cell Physiol. 2018 May 25. doi: 10.1093/pcp/pcy099.
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-mouse IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to all mouse IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody is adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,rabbit,rat,sheep IgG and affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins and bovine,chicken,goat,guinea pig,hamster,horse, human,rabbit,rat,sheep IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-rat IgG (H&L), Rhodamine (TRITC - tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds all rat IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody is adsorbed to bovine,chicken,goat,guinea pig,hamster,horse,human,mouse,rabbit,sheep IgG and affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, or sheep based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-sheep IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibody which binds to sheep IgGs in immunological assays. Antibodies are adsorbed against human,mouse,rabbit IgG and are affinity purified using solid phase sheep IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Chicken anti-goat IgG (H&L), biotin conjugated is a secondary antibody which binds to goat IgGs in immunological assays. Antibodies are affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgY.
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-goat IgG (H&L), ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to goat IgGs in immunological assays. Antibodies are affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-human IgA + IgG + IgM, affinity purified F(ab)'2 fragment, is a secondary antibody which binds to human IgA and IgG and IgM in immunological assays. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
This antibody reacts with the heavy and light chains on human IgA + IgG + IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, H 7, 0.05 % (w/v) sodium azide as preservative.
Goat anti-rat IgG (H&L), HRP conjugated is a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.!!AIR8!!Store liquid material at 2-8°Cupto 6 months.
Host Animal:
Goat
Species Reactivity:
Heavy chains on rat IgG and with the light chains on all rat immunoglobulins
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Not reactive in:
Non-immunoglobulin rat serum proteins
Selected references:
Saintenac et al. (2021) A wheat cysteine-rich receptor-like kinase confers broad-spectrum resistance against Septoria tritici blotch. Nat Commun. 2021 Jan 19;12(1):433. doi: 10.1038/s41467-020-20685-0. PMID: 33469010; PMCID: PMC7815785.
Special application note:
Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG as preservative.
Goat anti-rat IgG (H&L),HRP conjugated is a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are affinity purified using solid phase rat IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Goat anti-mouse IgG (H&L), HRP conjugated, is a F(ab)'2 fragment, of a secondary antibody which binds to mouse IgG in immunological assays. Antibodies are adsorbed against human IgG/serum. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Rabbit anti-mouse IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to mouse IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against human serum and affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins, or human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgG (H&L), affinity purified, is a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are adsorbed against human IgG/serum and affinity purified using solid phase mouse IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis. Minimum cross-reactivity is observed with human IgG or serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgG (H&L), ALP (alkaline phosphatase) conjugated, is a F(ab)'2 fragment of a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are adsorbed against human IgG/serum. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins and human IgG/serum based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Donkey anti-sheep IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibody which binds to sheep IgGs in immunological assays. Antibodies are adsorbed against human,rabbit IgG and are affinity purified using solid phase sheep IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins and human and rabbit IgG based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-rabbit IgG (H&L), Rhodamine (TRITC-tetramethyl rhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to rabbit IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator, (ICC), immunohistochemistry ( IHC) (Flow cyt)
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgG Fc, Rhodamine (TRITC-tetramethyl rhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to human IgG Fc constant part in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase human IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains on human immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins. Minimum cross-reactivity is observed to human IgG, F(ab)'2 fragment based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-Goat IgG (H&L), DyLight 488 Conjugated,min. cross-reactivity to human, mouse, rabbit or rat IgG is a secondary antibody conjugated to DyLight 488, which binds to Goat IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Goat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with:, heavy (γ) chains on goat IgG, light chains on all goat immunoglobulinsNo reactivity is observed to: non-immunoglobulin goat serum proteins, IgG/serum from human, mouse, rabbit or rat.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-human IgM ( chain), F(ab)'2 fragment, biotin conjugated is a secondary antibody which binds to human IgM in immunological assays. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the mu chains on human IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed with IgG, F(ab)'2 fragment based on immunoelectrophoresis. No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
Donkey anti-Mouse IgG (H&L), DyLight 488 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG is a secondary antibody conjugated to DyLight 488, which binds to Mouse IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Mouse IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Maurya et al. (2017). Hedgehog signaling regulates ciliary localization of mouse odorant receptors. Proc Natl Acad Sci U S A. 2017 Oct 31;114(44):E9386-E9394. doi: 10.1073/pnas.1708321114.
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins, IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep
Goat anti-rabbit IgG (H&L),biotin conjugated, is a secondary antibody which binds to rabbit IgGs in immunological assays. Antibody is adsorbed against bovine,human,mouse IgG/serum and are affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis. Minimum cross-reactivity was observed with bovine, human and mouse serum or human and mouse IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgG (H&L), affinity purified, is a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are adsorbed against bovine,goat,human,rabbit,rat IgG and affinity purified using solid phase mouse IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins and bovine, goat, human, rabbit and rat IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to mouse IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Rabbit anti-hamster IgG (H&L), TRITC (tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to hamster IgG in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Fluor/protein ratio is 1-2 moles TRITC per mole antibody. Antibodies are affinity purified on solid phase hamster IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on hamsterIgG and with the light chains on all hamsterimmunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin hamster serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-rat IgG (H&L), biotin conjugated is a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-rat IgG (H&L), biotin conjugated, is a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are adsorbed against human,mouse IgG and affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or to human or mouse IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-goat IgG (H&L), biotin conjugated, is a secondary antibody which binds to goat IgGs in immunological assays. Antibodies are adsorbed against human,mouse,rabbit,rat IgG and affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse, rabbit, or rat based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-rat IgG (H&L), affinity purified, is a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,mouse,rabbit,sheep IgG and are affinity purified using solid phase rat IgG. Antibody purity is > 95% based on SDS-PAGE.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, or sheep by immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative. 8-12 mg/ml
Goat anti-rat IgG (H&L), F(ab)'2 fragment, affinity purified, is a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are adsorbed against human, mouse IgG. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from human or mouse based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Goat anti-rabbit IgG (H&L), DyLight 488 Conjugated, min. cross-reactivity to bovine, goat, human, mouse, rat IgG or serum proteins is a secondary antibody conjugated to DyLight 488, which binds to Rabbit IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rabbit IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG, light chains on all rabbit immunoglobulinsNo reactivity is observed to: non-immunoglobulin rabbit serum proteins, serum proteins from bovine, goat, human, mouse or rat IgG from bovine, goat, human, mouse or rat
Goat anti-Rat IgG (H&L) - F(ab)'2 fragment, DyLight488 Conjugated, min. cross-reactivity to human, mouse lgG is a secondary antibody conjugated to DyLight 488, which binds to Rat IgG (H&L) - F(ab)'2 fragment in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG, light chains on all rat immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins, IgG from human or mouse
Donkey anti-rat IgG (H&L), affinity purified is a secondary antibody which binds to rat IgG in immunological assays. Antibodies are affinity purified using solid phase rat IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins
Goat anti-guinea pig IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to guinea pig IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase guinea pig IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on guinea pig IgG and with the light chains on all guinea pig immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin guinea pig serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgA + IgG + IgM, ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to human IgA and IgG and IgM F(ab)'2 fragment in immunological assays. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy and light on human IgA + IgG + IgM based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to mouse IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.Concentration: 1.50 mg/ml (E 1% at 280 nm = 13.0)
Chicken anti-rat IgG (H&L),HRP conjugated is a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgY.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Wilmowicz et al. (2022) Remodeling of Cell Wall Components in Root Nodules and Flower Abscission Zone under Drought in Yellow Lupine. Int J Mol Sci. 2022 Jan 31;23(3):1680. doi: 10.3390/ijms23031680. PMID: 35163603; PMCID: PMC8836056.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Donkey anti-mouse IgG (H&L), biotin conjugated is a secondary antibody which binds to mouse IgG in immunological assays. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-sheep IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody with binds to sheep IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against human,rabbit IgG and are affinity purified using solid phase sheep IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins and human,rabbit IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-mouse IgG Fc,HRP conjugated is a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on mouse IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Donkey anti-goat IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to goat IgG in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against human,mouse,rabbit,rat IgG and affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse, rabbit, or rat based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-rabbit IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to rabbit IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against bovine,human,mouse IgG/serum and are affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins and bovine,human,mouse IgG/serum based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-rabbit IgG Fc, FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which finds to rabbit IgG Fc part in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on rabbit IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains on rabbit IgG or to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Chicken anti-rat IgG (H&L), affinity purified is a secondary antibody which binds to rat IgG in immunological assays. Antibodies are affinity purified using solid phase rat IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgY.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Goat anti-rabbit IgG (H&L), affinity purified, is a secondary antibody which binds to rabbit IgG in immunological assays. Antibodies are adsorbed against bovine,human,mouse IgG/serum and affinity purified using solid phase rabbit IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins and bovine,human,mouse IgG/serum based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Goat anti-rat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, F(ab)'2 fragment of a secondary antibody which binds to rat IgG in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against human,mouse IgG. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from human or mouse based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Rabbit anti-mouse IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are adsorbed against human serum and affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse or human serum proteins based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgG Fc, , FITC-fluorescein-5-isothiocyanate conjugated is a F(ab)'2 fragment of a secondary antibody which binds to human IgG in immunological assay. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis. Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgM ( chain), ALP (alkaline phosphatase) conjugated is a F(ab)'2 fragment of a secondary antibody which binds to human IgM in immunological assays. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with:heavy ( ) chains on human IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed to: non-immunoglobulin serum proteins human IgG.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.Concentration: 1.50 mg/ml (E 1% at 280 nm = 13.0)
Chicken anti-goat IgG (H&L), HRP conjugated, is a secondary antibody which binds to goat IgGs in immunological assays. Antibodies are adsorbed against to human,mouse,rabbit IgG/serum and affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins and human,mouse,rabbit IgG/serum based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-human IgM ( chain), biotin conjugated, is a secondary antibody which binds to human IgM in immunological assays. Antibodies are adsorbed against human IgA or IgG and affinity purified using solid phase human IgM.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with:heavy ( ) chains on human IgM based on immunoelectrophoresis,Minimum cross-reactivity is observed to: non-immunoglobulin human serum proteins IgG, F(ab)'2 fragment human IgA or IgG.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Rabbit anti-goat IgG Fc, biotin conjugated is a secondary antibody which binds to goat IgG Fc constant part in immunological assays. Antibodies are affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on goat IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains on goat immunoglobulins or to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and and 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-mouse IgG (H&L), biotin conjugated, is a secondary antibody which binds to mouse IgGs in immunological asasys. Antibodies are adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,rabbit,rat,sheep IgG and affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins and bovine,chicken,goat,guinea, pig,hamster,horse,human,rabbit,rat,sheep IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and and 0.05 % (w/v) sodium azide as preservative.
Chicken anti-goat IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to goat IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed againt human,mouse,rabbit IgG/serum and affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgY.
Special application note:
Antibody reacts with heavy chains on goat IgG and light chains on all goat immunoglobulins.Minimum cross reactivity is observed to non-immunoglobulin goat serum proteins and human,mouse and rabbit IgG/serum based on immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-human IgA + IgG + IgM, biotin conjugated is a F(ab)'2 fragment of a secondary antibody which binds to human IgA and IgG and IgM. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on human IgA + IgG + IgM and with the light chains on all human immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-mouse IgG (H&L), affinity purified is a secondary antibody which binds to mouse IgG in immunological assay. Antibodies are affinity purified using solid phase mouse IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to human IgG in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against bovine,mouse,rabbit serum and affinity purified using solid phase human IgG. (H&L)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human, mouse, rabbit or bovine serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-Sheep IgG (H&L), DyLight 488 Conjugated, min. cross-reactivity to human,mouse, or rabbit IgG is a secondary antibody conjugated to DyLight 488, which binds to Sheep IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Sheep IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8 C, Product is stable for 4 weeks at 2-8°Cafter rehydration,For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity, For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol, Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol, Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG, light chains on all sheep immunoglobulinsNo reactivity is observed to: non-immunoglobulin sheep serum proteins, IgG from human, mouse or rabbit. Cross-reactivity to goat IgG has not been tested. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-rat IgG (H&L), biotin conjugated is a secondary which binds to rat IgGs in immunological assays. Antibodies are affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Selected references:
Lopez et al. (2018). Evasion of Immune Surveillance in Low Oxygen Environments Enhances Candida albicans Virulence. MBio. 2018 Nov 6;9(6). pii: e02120-18. doi: 10.1128/mBio.02120-18.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and and 0.05 % (w/v) sodium azide as preservative.
Chicken anti-goat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to goat IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against human,mouse,rabbit IgG/serum and are affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-goat IgG (H&L), HRP conjugated is a secondary antibody which binds to goat IgGs in immunological assays. Antibodies are affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.BSA and milk have to be replaced by other blocking reagents, like donkey serum or commercial formulations which are free from bovine IgG.Concentration: 1.0 mg/ml.
Donkey anti-Mouse IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Mouse IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Mouse IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins
Chicken anti-goat IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibody which binds to goat IgGs in immunological assays. Antibodies are adsorbed against human,mouse,rabbit IgG/serum and are affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins and human,mouse,rabbit IgG/serum based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-rabbit IgG (H&L), affinity purified is an unconjugated secondary antibody which binds to rabbit IgGs in immunological assays. Antibodies are adsorbed against mouse IgG/serum and affinity purified using solid phase rabbit IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed with non-immunoglobulin rabbit or mouse serum proteins or mouse IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Rabbit anti-goat IgG (H&L), affinity purified is a secondary antibody which binds to goat IgGs in immunological assays. Antibodies are affinity purified using solid phase goat IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-rat IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to rat IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator, Usually 1 : 100-1 : 500,
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis. Please include 5 % goat serum in secondary antibody diluent buffer. Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Chicken anti-rabbit IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to rabbit IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against human,mouse IgG and affinity purified.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgY.
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-guinea pig IgG (H&L), biotin conjugated, is a secondary antibody which binds to guinea pig IgGs in immunological assays. Antibodies are adsorbed against bovine,chicken,goat,hamster,horse,human,mouse,rabbit,rat,sheep serum and affinity purified using solid phase guinea pig IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on guinea pig IgG and with the light chains on all guinea pig immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin guinea pig serum proteins and bovine,chicken,goat,hamster,horse,human,mouse,rabbit,rat,sheep serum based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-rabbit IgG (H&L), TRITC (tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to rabbit IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Rabbit anti-goat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to goat IgGs in immunological assays. Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against human,mouse,rat IgG and affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or IgG from human, mouse or rat based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-rabbit IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibody which binds to rabbit IgGs in immunological assays. Antibodies are adsorbed against bovine,human,mouse IgG/serum and purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilution prior to use and then discard. Shelf life of this product is one year from date of receipt.
This antibody reacts with:heavy chains on rabbit IgG light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to: non-immunoglobulin rabbit serum proteins serum/IgG proteins from bovine, human and mouse.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgG Fc, FITC -fluorescein-5-isothiocyanate conjugated is a secondary antibody which binds to mouse IgG Fc constant part in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on mouse IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Rabbit anti-goat IgG Fc, affinity purified, is a secondary antibody which binds to goat IgG Fc constant part in immunological assays. Antibodies are adsorbed against human serum and are affinity purified using solid phase goat IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on goat IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins or immunoglobulin light chains based on immunoelectrophoresis. Minimum cross-reactivity is observed with human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-human IgA + IgG + IgM, , FITC (fluorescein-5-isothiocyanate) conjugated is a F(ab)'2 fragment of a secondary antibody which binds to human IgA and IgG and IgM in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy and light chains on human IgA, IgG and IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-mouse IgG (H&L),HRP conjugated is a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Chicken anti-rat IgG (H&L), ALP (alkaline phosphatase) conjugated, is a secondary antibody which binds to rat IgGs in immunoglogical assays. Antibodies are adfsorbed against human,rabbit IgG/serum and are affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Rabbit anti-goat IgG Fc, alkaline phosphatase (ALP) conjugated is a secondary antibody which binds to goat IgG Fc part in immunological assays. Antibodies are adsorbed against human serum and affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on goat IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed to the light chains or to non-immunoglobulin goat serum proteins based on immunoelectrophoresis. Minimum cross-reactivity is observed to human serum proteins based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-rat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to rat IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,mouse, rabbi,sheep IgG and affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Wijnker et al. (2019). The Cdk1/Cdk2 homolog CDKA;1 controls the recombination landscape in Arabidopsis. Proc Natl Acad Sci U S A. 2019 Jun 18;116(25):12534-12539. doi: 10.1073/pnas.1820753116.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, or sheep based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Chicken anti-rat IgG (H&L), biotin conjugated, is a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are adsorbed against human,rabbit IgG/serum and affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgY.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins and human,rabbit IgG/serumbased on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-chicken IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated i a secondary antibody which binds to chicken IgG (called also chicken IgY) in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase chicken IgY (called also chicken IgG). (H&L)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on chicken IgG and with the light chains on all chicken immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin chicken serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-mouse IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to mouse IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgA + IgG + IgM, ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to human IgA and IgG and IgM in immunological assays. Antibodies are affinity purified using solid phase human IgA + IgG + IgM.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy and light on human IgA + IgG + IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgM ( chain), FITC (fluorescein-5-isothiocyanate) conjugated, is a F(ab)'2 fragment of a secondary antibody which binds to human IgM in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against human IgG or IgA and affinity purified using solid phase human IgM.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the mu chains on human IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed with IgG, F(ab)'2 fragment, IgA or IgG based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgG (H&L), affinity purified is a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are adsorbed against bovine, goat, human, rabbit or rat IgG and highly adsorbed against rat IgG and affinity purified using solid phase mouse IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Highly cross absorbed against rat IgG.Minimum cross-reactivity is observed to non- immunoglobulin mouse serum proteins based on immunoelectrophoresis. Minimum cross-reactivity was observed with IgG from bovine, goat, human, Rabbit or rat based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated is a F(ab)'2 fragment of a secondary antibody which binds to mouse IgG in immunological assay. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgM ( chain), Rhodamine (TRITC -tetramethylrhodamine-5-isothiocyanate) conjugated is a secondary antibody which binds to human IgM in immunological assays. TRITC Amax = 550 nm, Emax = 570 nm. Antibodies affinity purified using solid phase human IgM.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the mu chains on human IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed with IgG, F(ab)'2 fragment, IgA or IgG based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Rabbit anti-mouse IgG (H&L), biotin conjugated, is a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are adsorbed against human serum. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins, or human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-sheep IgG (H&L), affinity purified, is a secondary antibody which binds to sheep IgGs in immunological assays. Antibodies are adsorbed against human,mouse,rabbit IgG and affinity purified using solid phase sheep IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins and human,mouse,rabbit IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-sheep IgG (H&L), affinity purified, is an unconjugated secondary antibody which binds to sheep IgGs in immunological assays. Antibodies are adsorbed against human,rabbit IgG and affinity purified using solid phase sheep IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-mouse IgG Fc, ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to mouse IgG Fc part in immunological assays. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on mouse IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins. Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a F(ab)'2 fragment of a secondary antibody which binds to human IgG in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgM, ( chain), affinity purified is a F(ab)'2 fragment of an unconjugated, secondary antibody which binds to human IgM in immunological assays. Antibodies are adsorbed against human IgA and IgG and affinity purified. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Goat anti-human IgM ( chain), ALP (alkaline phosphatase) conjugated, is a F(ab)'2 fragment of a secondary antibody which binds to human IgM in immunological assays. Antibodies are adsorbed against human IgG or IgA. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with:heavy ( ) chains on human IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed to: non-immunoglobulin serum proteins human IgG or IgA.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Donkey anti-rabbit IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to rabbit IgG in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Kozieł et al. (2017). Subcelullar localization of proteins associated with Prune dwarf virus replication. Eur. J. Pathology DOI: 10.1007/s10658-017-1215-8.
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgE (epsilon chain), FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which binds to human IgE in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase human IgE.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the epsilon chains on human IgE based on immunoelectrophoresis.No reactivity is observed to the light chains or non-IgE human serum proteins based on immunoelectrophoresis.
Donkey anti-goat IgG (H&L), biotin conjugated is a secondary antibody which binds to goat IgG in immunological assays. Antibodies are affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-human IgA + IgG + IgM,Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated is a F (ab)'2 fragment of a secondary antibody which binds to human IgA and IgG and IgM in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy and light chains on human IgG, IgA and IgM based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.
Affinity purified using solid phase human C1 inhibitor. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0).Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative. Antibody purity is > 95% based on SDS-PAGE.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen Affinity purified using solid phase human C1 Inhibitor.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
CKMM can be found in human heart and is an important marker of important serum marker myocardial infarction. Alternative names: creatine kinase M-chain, M-CK.
Affinity purified using solid phase human CKMM. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0).Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative. Antibody purity is > 95% based on SDS-PAGE.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified using solid phase human CKMM.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Affinity purified using solid phase human plasminogen. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0).Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative. Antibody purity is > 95% based on SDS-PAGE.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen Affinity purified using solid phase human Plasminogen.
Molecular Weight:
95 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Purified human IgM contains purified human IgM from normal serum from patients with myeloma which express IgM and is excellent for use as blocking reagent in immunoassays.
To be used as a blocking reagent, normal control, or standard in most immunoassay protocols, The optimal working dilution should be determined by the investig
Purity:
Immunogen affinity purified human IgM.
Special application note:
Concentration: >1.0 mg/ml (E 1% at 280 nm = 14.0)Purity of this preparation is > 95% based on SDS-PAGE. Antibody is provided as a clear, colorless liquid, 0.2 m filtered in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.05 % sodium azide as preservative, 0.2 m filtered.Based on IEP, precipitin bands occur when reacted against antibodies to: Whole human serum, human IgM, Kappa light chain.Based on IEP, this protein does not form precipitin bands against antibodies to: human IgA, IgG, IgD, IgE or lambda light chain.
Goat anti-human kappa (k) chain is a secondary antibody which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: kappa (k) light chains on human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins.
Goat anti-human kappa (k) chain is a secondary antibody conjugated to ALP, which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with:kappa (k) light chains on human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to:non-immunoglobulin human serum proteins,heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins.
Goat anti-human kappa (k) chain is a secondary antibody conjugated to biotin, which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 2000-1 : 20 000 (WB) 1 : 500 - 1 : 5 000 (IHC)
Purity:
Immunogen affinity purified goat IgG.
Selected references:
Reinhart et al. (2014). In search of expression bottlenecks in recombinant CHO cell lines-a case study. Appl Microbiol Biotechnol. 2014 Feb 21.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: kappa (k) light chains on human immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins.
Goat anti-human kappa (k) chain is a secondary antibody conjugated to FITC, which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain. Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm, Emax = 518 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
(Flow cyt) The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: kappa (k) light chains on human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins.
Goat anti-human kappa (k) chain is a secondary antibody conjugated to HRP, which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 5 000 (IHC), 1 : 200-1 : 5000 (WB)
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: kappa (k) light chains on human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins.
Goat anti-human kappa (k) chain is a secondary antibody conjugated to TRITC, which binds to human kappa (k) chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain. Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: kappa (k) light chains on human immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins.
Goat anti-human kappa (k) chain is a secondary antibody which binds to human kappa (k) chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain.
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: kappa (k) light chains on human immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to:non-immunoglobulin human serum proteins heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins, mouse serum proteins.
Goat anti-human kappa chain is a secondary antibody conjugated to biotin, which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 2000-1 : 20 000 (WB) 1 : 500-1 : 5 000 (IHC)
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: kappa light chains on human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to:non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins,mouse serum proteins.
Goat anti-human kappa (k) chain is a secondary antibody conjugated to FITC, which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain. Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with:kappa light chains on human immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins, mouse serum proteins.
Goat anti-human kappa (k) chain is a secondary antibody conjugated to HRP, which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Naderi et al. (2018). The Augmenting Effects of the tDNA Insulator on Stable Expression of Monoclonal Antibody in Chinese Hamster Ovary Cells. Monoclon Antib Immunodiagn Immunother. 2018 Nov;37(5):200-206. doi: 10.1089/mab.2018.0015.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: kappa light chains on human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to:non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins,mouse serum proteins.
Goat anti-llama IgG (H&L) is a secondary antibody which binds to llama immunoglobulins (H&L) in immunological assays. Antibody is affinity purified using solid phase Llama IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on llama IgG, light chains on all llama immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin llama serum proteins.This antibody will react with VHH of llama IgG's.
Goat anti-llama IgG (H&L) is a secondary antibody conjugated to ALP, which binds to llama immunoglobulins (H&L) in immunological assays. Antibody is affinity purified using solid phase Llama IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on llama IgG, light chains on all llama immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin llama serum proteins.This antibody will react with VHH of llama IgG's.
Goat anti-llama IgG (H&L) biotinylated is a secondary antibody conjugated to biotin, which binds to llama immunoglobulins (H&L) in immunological assays. Antibody is affinity purified using solid phase Llama IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 2000-1 : 20 000 (WB) 1 : 500-1 : 5 000 (IHC)
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with:heavy chains on llama IgG, light chains on all llama immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin llama serum proteins.This antibody will react with VHH of llama IgG's.
Goat anti-llama IgG (H&L), FITC conjugated, is a secondary antibody conjugated to FITC, which binds to llama IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase Llama IgG (H&L). Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications.
Purity:
Immunogen affinity purified goat IgG.
Selected references:
Meyer et al. (2014). Antibodies against MERS coronavirus in dromedary camels, United Arab Emirates, 2003 and 2013. Emerg Infect Dis. 2014 Apr;20(4):552-9. doi: 10.3201/eid2004.131746.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with heavy chains on llama IgG and light chains on all llama immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin llama serum proteins.This antibody will react with VHH of llama IgGs.
Goat anti-llama IgG (H&L) HRP conjugated is a secondary antibody conjugated to HRP, which binds to llama IgG (H&L) and will recognize llama VHH domain in immunological assays. Antibody is affinity purified using solid phase llama IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Heavy chains on llama IgG and light chains on all llama immunoglobulins
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Not reactive in:
Non-immunoglobulin llama serum proteins
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.The antibody is not adsorbed against other species IgGs, therefore it might react with rabbit immunoglobulins.This antibody will react with VHH of llama IgG's.
Goat anti-llama IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to llama immunoglobulins (H&L) in immunological assays. Antibody is affinity purified using solid phase llama IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with:heavy chains on llama IgG, light chains on all llama immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin llama serum proteins.This antibody will react with VHH of llama IgG's.
Goat anti-llama IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to llama IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Llama IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on llama IgG, light chains on all llama immunoglobulinsThis antibody will react with VHH of llama IgG's.No reactivity is observed to: non-immunoglobulin llama serum proteins
Goat anti-mouse IgG (H&L) is a secondary antibody which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phaseMouse IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 14.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with:heavy chains on mouse IgG, light chains on all mouse immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins,serum proteins from bovine, horse, human, pig, or rabbit.
Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to biotin, which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phaseMouse IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 2000-1 : 20 000 (WB) 1 : 500-1 : 5 000 (IHC)
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on mouse IgG, light chains on all mouse immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig, or rabbit.
Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to FITC, which binds to mouse IgG in immunological assays. Antibody is affinity purified using solid phase mouse IgG (H&L). Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on mouse IgG, light chains on all mouse immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig, or rabbit.
Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to HRP, which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phaseMouse IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Goat
Immunogen:
Purified mouse IgG (H&L) AAA51107
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Barahimipour et al. (2016). Efficient expression of nuclear transgenes in the green alga Chlamydomonas: synthesis of an HIV antigen and development of a new selectable marker. Plant Mol Biol. 2016 Mar;90(4-5):403-18. doi: 10.1007/s11103-015-0425-8. Epub 2016 Jan 8.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with:heavy chains on mouse IgG, light chains on all mouse immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig, or rabbit.
Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to HRP, which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phaseMouse IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store liquid material at 2-8°Cup to 6 months.
Host Animal:
Goat
Immunogen:
Purified mouse IgG (H&L) AAA51107
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Barahimipour et al. (2016). Efficient expression of nuclear transgenes in the green alga Chlamydomonas: synthesis of an HIV antigen and development of a new selectable marker. Plant Mol Biol. 2016 Mar;90(4-5):403-18. doi: 10.1007/s11103-015-0425-8. Epub 2016 Jan 8.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with:heavy chains on mouse IgG, light chains on all mouse immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig, or rabbit.
Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phaseMouse IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with:heavy chains on mouse IgG, light chains on all mouse immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum proteins, serum proteins from bovine, horse, human, pig, or rabbit.
Goat anti-biotin is a secondary antibody conjugated to ALP, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE, Antibody concentration is 1,50 mg/ml (E 1% at 280 nm = 13,0), Antibody is supplied in 30 mM triethanolamine, pH 7,2,5 mM magnesium chloride, 0,1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free, Contains 0,05% (w/v) sodium azide as preservative of bacterial growth
Goat anti-biotin is a secondary antibody conjugated to FITC, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin. Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE, Antibody concentration is 1,0 mg/ml (E 1% at 280 nm = 13,0), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2,1 % (w/v) B, Protease/IgG free, Contains 0,05% (w/v) sodium azide as preservative of bacterial growth
Goat anti-biotin is a secondary antibody conjugated to HRP, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE, Antibody concentration is 1,0 mg/ml, Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2,1 % (w/v) B, Protease/IgG free, Contains 0,1 % (v/v) Kathon CG as preservative of bacterial growth
Goat anti-biotin is a secondary antibody conjugated to TRITC, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin. Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE, Antibody concentration is 1,0 mg/ml (E 1% at 280 nm = 13,0), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2,1 % (w/v) B, Protease/IgG free, Contains 0,05% (w/v) sodium azide as preservative of bacterial growth
Goat anti-rabbit IgG (H&L) is a secondary antibody which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 14.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, human serum proteins.
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to ALP, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE, Antibody concentration is 1,50 mg/ml (E 1% at 280 nm = 13,0), Antibody is supplied in 30 mM triethanolamine, pH 7,2,5 mM magnesium chloride, 0,1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free, Contains 0,05% (w/v) sodium azide as preservative of bacterial growth, Based on immunoelectrophoresis,this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins, Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, human serum proteins
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to biotin, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, human serum proteins.
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Sun et al. (2019). Single-Organelle Quantification Reveals Stoichiometric and Structural Variability of Carboxysomes Dependent on the Environment. Plant Cell. 2019 Jul;31(7):1648-1664. doi: 10.1105/tpc.18.00787.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, human serum proteins.
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin rabbit serum proteins, human serum proteins.
Goat anti-rabbit IgG (H&L) is a secondary antibody which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 14.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, serum proteins from human, mouse or rat.
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to ALP, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with:heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, serum proteins from human, mouse or rat.
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to biotin, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, serum proteins from human, mouse or rat.
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to FITC, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L). Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins serum proteins from human, mouse or rat
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, serum proteins from human, mouse or rat
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, serum proteins from human, mouse or rat
Rabbit anti-bovine IgG (H&L) is a secondary antibody conjugated to FITC, which binds to bovine IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase bovine IgG (H&L). Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on bovine IgG light chains on all bovine immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin bovine serum proteins
Rabbit anti-cat IgG (H&L) is a secondary antibody which binds to cat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase cat IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on cat IgG light chains on all cat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin cat serum proteins
Rabbit anti-chicken IgG/Y (H&L) is a secondary antibody which binds to chicken IgG/Y (H&L) in immunological assays. Antibody is affinity purified using solid phase chicken IgG/Y.
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on chicken IgG (IgY), light chains on all chicken immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin chicken serum proteins
Rabbit anti-chicken IgG/Y (H&L) is a secondary antibody conjugated to FITC, which binds to chicken IgG/Y (H&L) in immunological assays. Antibody is affinity purified using solid phase chicken IgG/Y. Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on chicken IgG (IgY), light chains on all chicken immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin chicken serum proteins
Rabbit anti-chicken IgY (H&L) is a secondary antibody conjugated to HRP, which binds to in immunological assays. Antibody is affinity purified using solid phase chicken IgY.Chicken egg yolk immunoglobulin (IgY) has been also called chicken IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Rabbit
Species Reactivity:
Heavy chains on chicken IgY and light chains on all chicken immunoglobulins,
Immunogen:
Purified Chicken IgY (H&L), whole molecule
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Not reactive in:
Non-immunoglobulin chicken serum proteins
Selected references:
Li et al. (2022), The effects of Ni availability on H2 production and N2 fixation in a model unicellular diazotroph: The expression of hydrogenase and nitrogenase. Limnol Oceanogr, 67: 1566-1576. https://doi.org/10.1002/lno.12151Panayiotou et al. (2018). Viperin restricts Zika virus and tick-borne encephalitis virus replication by targeting NS3 for proteasomal degradation.J Virol. 2018 Jan 10. pii: JVI.02054-17. doi: 10.1128/JVI.02054-17.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.
Rabbit anti-chicken IgG/Y (H&L) is a secondary antibody conjugated to HRP, which binds to in immunological assays. Antibody is affinity purified using solid phase chicken IgG/Y.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized material at 2-8°C. For storage at -20 °C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 5 000 (IHC), 1 : 200-1 : 5000 (WB)
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on chicken IgG (IgY), light chains on all chicken immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin chicken serum proteins
Rabbit anti-chicken IgG/Y (H&L) is a secondary antibody conjugated to TRITC , which binds to chicken IgG/Y (H&L) in immunological assays. Antibody is affinity purified using solid phase chicken IgG/Y. Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on chicken IgG (IgY), light chains on all chicken immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin chicken serum proteins
Rabbit anti-guinea pig IgG (H&L) is a secondary antibody which binds to guinea pig IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase guinea pig IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on guinea pig, IgG light chains on all guinea pig immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin guinea pig serum proteins
Rabbit anti-guinea pig IgG (H&L) is a secondary antibody conjugated to FITC, which binds to guinea pig IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase guinea pig IgG (H&L). Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Selected references:
Sergey Mursalimov et al. (2022) Cytomixis in wheat male meiosis: influence analysis of the substitution of chromosome 1A, 2D, 5A, or 5D, Botany Letters, DOI: 10.1080/23818107.2022.2074889
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on guinea pig IgG, light chains on all guinea pig immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin guinea pig serum proteins.
Rabbit anti-guinea pig IgG (H&L) is a secondary antibody conjugated to HRP, which binds to guinea pig IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase guinea pig IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on guinea pig IgG, light chains on all guinea pig immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin guinea pig serum proteins
Rabbit anti-guinea pig IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to guinea pig IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase guinea pig IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on guinea pig IgG, light chains on all guinea pig immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin guinea pig serum proteins
Rabbit anti-human IgG (H&L) is a secondary antibody which binds to human IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgG, light chains on all human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins.
Rabbit anti-human IgG (H&L) is a secondary antibody conjugated to ALP, which binds to human IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgG, light chains on all human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins
Rabbit anti-human IgG (H&L) is a secondary antibody conjugated to biotin, which binds to human IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 2000-1 : 20 000 (WB) 1 : 500-1 : 5 000 (IHC)
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgG, light chains on all human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins
Rabbit anti-human IgG (H&L) is a secondary antibody conjugated to FITC, which binds to human IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L). Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgG, light chains on all human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins.
Rabbit anti-human IgG (H&L) is a secondary antibody conjugated to HRP, which binds to human IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgG, light chains on all human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins.
Rabbit anti-human IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to human IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgG, light chains on all human immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins
Rabbit anti-human IgM ( chain) is a secondary antibody which binds to human IgM (m chain) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgM Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgM Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins
Application Details:
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 2000-1 : 20 000 (WB) 1 : 500 - 1 : 5 000 (IHC)
Rabbit anti-human IgM ( chain) is a secondary antibody conjugated to FITC, which binds to human IgM in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L). Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy (m) chains on human IgM. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins, light chains on all human immunoglobulins
Rabbit anti-human IgM ( chain) is a secondary antibody conjugated to HRP, which binds to human IgM (heavy chain) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy ( ) chains on human IgM Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins, light chains on all human immunoglobulins
Rabbit anti-human IgM ( chain)is a secondary antibody conjugated to TRITC, which binds to human IgM in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgM. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins.
Rabbit anti-llama IgG (H&L) is a secondary antibody which binds to llama IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase llama IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on llama IgG, light chains on all llama immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin llama serum proteins.This antibody will react with VHH of llama IgG's.
Rabbit anti-llama IgG (H&L) is a secondary antibody conjugated to ALP, which binds to in immunological assays. Antibody is affinity purified using solid phase llama IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)
Purity:
Immunogen affinity purified rabbit IgG.
Selected references:
Alharbi et al. (2019). Humoral Immunogenicity and Efficacy of a Single Dose of ChAdOx1 MERS Vaccine Candidate in Dromedary Camels. Sci Rep. 2019 Nov 8;9(1):16292. doi: 10.1038/s41598-019-52730-4.Aharbi et al. (2019). Humoral Immunogenicity and Efficacy of a Single Dose of ChAdOx1 MERS Vaccine Candidate in Dromedary Camels. Sci Rep. 2019 Nov 8;9(1):16292. doi: 10.1038/s41598-019-52730-4.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on llama IgG, light chains on all llama immunoglobulins. Based on immunoelectrophoresis no reactivity is observed to: non-immunoglobulin llama serum proteins.This antibody will react with VHH of llama IgG's.
Rabbit anti-llama IgG (H&L) is a secondary antibody conjugated to biotin, which binds to llama IgG in immunological assays. Antibody is affinity purified using solid phase llama IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 2000-1 : 20 000 (WB) 1 : 500-1 : 5 000 (IHC)
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on llama IgG, light chains on all llama immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin llama serum proteins.This antibody will react with VHH of llama IgG's.
Rabbit anti-llama IgG (H&L) is a secondary antibody conjugated to FITC, which binds to llama IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase llama IgG (H&L). Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on llama IgG, light chains on all llama immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin llama serum proteins.This antibody will react with VHH of llama IgG's.
Rabbit anti-llama IgG (H&L) is a secondary antibody conjugated to HRP, which binds to llama IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase llama IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Rabbit
Immunogen:
Purified llama IgG (H&L) AAQ19986
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western Blot (WB)
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on llama IgG, light chains on all llama immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin llama serum proteins.This antibody will react with VHH of llama IgG's.
Rabbit anti-llama IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to llama IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Llama IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on llama IgG, light chains on all llama immunoglobulinsThis antibody will react with VHH of llama IgG's.No reactivity is observed to: non-immunoglobulin llama serum proteins
Rabbit anti-rat IgG (H&L) is a secondary antibody which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins.
Rabbit anti-rat IgG (H&L) is a secondary antibody conjugated to ALP, which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 2000 (IHC), 1 : 50 000-1 : 5 000 (WB)
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins.
Rabbit anti-rat IgG (H&L) is a secondary antibody conjugated biotin, which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 2000-1 : 20 000 (WB) 1 : 500-1 : 5 000 (IHC)
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins.
Rabbit anti-rat IgG (H&L) is a secondary antibody conjugated to FITC, which binds to anti-rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L). Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Selected references:
Kolbert et al. (2018). Nitro-oxidative stress correlates with Se tolerance of Astragalus species. Plant Cell Physiol. 2018 May 25. doi: 10.1093/pcp/pcy099.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins
Rabbit anti-rat IgG (H&L) is a secondary antibody conjugated to HRP, which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Quian et al. (2015). Bone Marrow-Derived Mesenchymal Stem Cells Repair Necrotic Pancreatic Tissue and Promote Angiogenesis by Secreting Cellular Growth Factors Involved in the SDF-1α/CXCR4 Axis in Rats. Stem Cells International Volume 2015 (2015), Article ID 306836.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins.
Rabbit anti-rat IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins.
Rabbit anti-rat IgG (H&L) is a secondary antibody which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins, human serum proteins
Rabbit anti-rat IgG (H&L) is a secondary antibody conjugated to biotin, which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 2000-1 : 20 000 (WB) 1 : 500-1 : 5 000 (IHC)
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins, human serum proteins
Rabbit anti-rat IgG (H&L) is a secondary antibody conjugated to HRP, which binds to rat IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rat IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 5 000 (IHC), 1 : 200-1 : 5000 (WB)
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins, human serum proteins.
Sheep anti-rabbit IgG (H&L) is a secondary antibody which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified sheep IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins.
Sheep anti-rabbit IgG (H&L) is a secondary antibody conjugated to FITC, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L). Fluorescein-5-isothiocyanate (FITC) has Amax = 494 nm; Emax = 518 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified sheep IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins.
Sheep anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 500-1 : 5 000 (IHC), 1 : 200-1 : 5000 (WB)
Purity:
Immunogen affinity purified sheep IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins.
Sheep anti-rabbit IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm; Emax = 570 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified sheep IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins.
Normal human serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
This product is prepared from a serum pool of donors.
Purity:
Serum
Reconstitution:
For reconstitution add 2,2 ml of sterile water
Special application note:
Protein concentration is 60,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Normal human serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
This product is prepared from a serum pool of donors.
Purity:
Serum
Reconstitution:
For reconstitution add 11,0 ml of sterile water
Special application note:
Protein concentration is 60,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Normal mouse serum. Lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 50,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Normal mouse serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 50,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Normal rabbit serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added.This product be used as a blocking reagent at 2-5% for primary antibodies developed in a rabbit.
Normal rabbit serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added.Rabbit serum can be used as a blocking reagent at 2-5% for primary antibodies developed in a rabbit. This product can be also used in flow cytometry. Serum has been delipidated and filtered using 0.22 m filter.
Normal goat serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 60,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Normal goat serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Pastula & Lundmark (2021). Induction of Epithelial-mesenchymal Transition in MDCK II Cells. Bio-protocol 11(3): e3903. DOI: 10.21769/BioProtoc.3903.
Special application note:
Protein concentration is 60,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Normal goat serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 60,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Normal goat serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 60,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Normal rat serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 50,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Normal rat serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 50,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Normal guinea pig serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 50,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Normal syrian hamster serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 50,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Normal syrian hamster serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 50,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Normal horse serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications. Blocking is a critical step in most immunoassays and "fills-in" the unoccupied spaces of the solid phase that are not occupied by immobilized proteins. Without blocking antibodies would bind non-specifically wich in turn could lead to false signaling and /or background issues. How to choose blocking serum? If you use a secondary from donkey e.g. donkey anti-chicken HRP (or other lable) - your blocking serum should be also coming from donkey. Serum from a species primary antibody is coming from should not be used as this will compete for binding sites with secondary antibodies. In this example serum from chicken should not be used as blocking reagent.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Usually 5 % (v/v) serum blocking buffer it is recommended and for that you need to mix 2 ml of blocking serum with 38 ml of diluent buffer (PBS with Tween 20 detergent) to obtain a total volume of 40 ml. Use immediately or store at 2-8 C or colder.
Purity:
Serum
Reconstitution:
For reconstitution add 2.2 ml of sterile water
Special application note:
Protein concentration is 60,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Normal horse serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications. Blocking is a critical step in most immunoassays and "fills-in" the unoccupied spaces of the solid phase that are not occupied by immobilized proteins. Without blocking antibodies would bind non-specifically wich in turn could lead to false signaling and /or background issues. How to choose blocking serum?If you use a secondary from donkey e.g. donkey anti-chicken HRP (or other lable) - your blocking serum should be also coming from donkey. Serum from a species primary antibody is coming from should not be used as this will compete for binding sites with secondary antibodies. In this example serum from chicken should not be used as blocking reagent.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Usually 5 % (v/v) serum blocking buffer it is recommended and for that you need to mix 2 ml of blocking serum with 38 ml of diluent buffer (PBS with Tween 20 detergent) to obtain a total volume of 40 ml, Use immediately or store at 2-8 C or colder,
Purity:
Serum
Reconstitution:
For reconstitution add 11,0 ml of sterile water
Special application note:
Protein concentration is 60,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Normal donkey serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Concentration: 60.0 mg/ml (Bradford, IgG standards)Recommended dilution: 2-5% (v/v) serum in PBS/TBS. To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.For a Western Blot 10-20 ml of blocking agent is needed, aliquote and freeze the rest of the unused blocking buffer, because there are no preservatives in the serum.Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added.
Normal donkey serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Host Animal:
Donkey
Applications:
ELISA (ELISA). Immunofluorescence (IF), Western blot (WB)
Recommended dilution: 2-5% (v/v) serum in PBS/TBS. To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.For a Western Blot 10-20 ml of blocking agent is needed, aliquote and freeze the rest of the unused blocking buffer, because there are no preservatives in the serum.Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added.As a blocking agent, this product is used to block non specific interaction with conjugates of donkey primary or secondary antibodies.
Normal pig (swine) serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 60,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Normal cat serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 60,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Normal cat serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 60.0 mg/ml (Bradford, IgG standards). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. No preservative is added. Serum may may contain anti-feline calicivirus antibodies. Not suitable as negative control within calicivirus assay.
Normal dog serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 60,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
Normal dog serum lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. This product is used as a blocking reagent or control for most immunoassay applications.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long term storage after reconstitution, prepare small aliquots and store at -20 °C. For storage at 2-8 C, add a preservative to prevent growth of bacteria. Rehydrate with 10,0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Protein concentration is 60,0 mg/ml (Bradford, IgG standards), Antibody is supplied in 10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, No preservative is added
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient. The transmembrane CF0IV subunit of appr. 25 kDa belongs to a stator part of ATP synthase and is involved in the proton translocation.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4 C, Long-term -20 . Repeated freezing and thawing is not recommended. It ontains 0,01% sodium azide.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.
Serine-threonine protein kinase with molecular weight of 56 kDa and known as TAK-kinase was found in chloroplast thylakoid membranes. It was initially suggested that TAK-kinases (TAK 1 – 3) could be involved in the phosphorylation of LHCII and photosystem II proteins.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4 C, Long-term -20 . Repeated freezing and thawing is not recommended. It contains 0.01% sodium azide.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Oryza sativa, Zea mays, Physcomitrium patens, Populus trichocarpa, Ricinus communis, Sorghum bicolor, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
Recombinant serine/threonine protein kinase from Arabidopsis thaliana TAIR:At4g02630, UniProt: O22764,
Polyphenol oxidase participates in the response of plants to wounding and herbivore attack, mediated by the octadecanoid wound-signalling pathway. Chloroplast polyphenol oxidase is a nuclear-encoded protein that is targeted to the thylakoid lumen. It was found that polyphenol oxidase is one of the most strongly phosphorylated protein in thylakoid lumen although the role of this protein modification is not known. Alternative name: catechol oxidase.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4 C, Long-term -20 . Repeated freezing and thawing is not recommended. It ontains 0,01% sodium azide.
Host Animal:
Rabbit
Species Reactivity:
Spinacia oleracea
Expected Species:
Spinacia oleracea
Immunogen:
recombinant lumenal polyphenol oxidase of Spinacia oleracea UniProt: P43310
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4 C, Long-term -20 . Repeated freezing and thawing is not recommended. It ontains 0,01% sodium azide.
Host Animal:
Rabbit
Species Reactivity:
dicots, Chlamydomonas reinhardtii, cyanobacteria including Synechocystis sp. PCC 6803.
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
isolated CF1 subunit of the chloroplast ATP synthase complex from Chlamydomonas reinhardtii
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4 C, Long-term -20 . Repeated freezing and thawing is not recommended. It ontains 0,01% sodium azide.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Ostreococcus lucimarinusm, Spinacia oleracea, Sorghum bicolor, Volvox carteri Species of your interest not listed? Contact us
Immunogen:
isolated CF1 subunit of the chloroplast ATP synthase complex from Chlamydomonas reinhardtii Q42687.1
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Perlaza (2021). Organelle Size and Quality Control in Chlamydomonas Reinhardtii. UCSF. ProQuest ID: Perlaza_ucsf_0034D_12217. Merritt ID: ark:/13030/m5257z1d. Retrieved from https://escholarship.org/uc/item/1jg3874hPerlaza et al. (2019). The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response. Elife. 2019 Oct 15;8. pii: e49577. doi: 10.7554/eLife.49577. (immunofluorescence)
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at at Store short-term 4 C, Long-term -20 . Repeated freezing and thawing is not recommended.
This product can be sold with ProClin if requested
Application Details:
1 : 2000 (WB)
Purity:
Serum
Molecular Weight:
23 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Blair et al. (2018). The Helicobacter pylori cell shape promoting protein Csd5 interacts with the cell wall, MurF, and the bacterial cytoskeleton. Mol Microbiol. 2018 Jul 24. doi: 10.1111/mmi.14087.Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.
SppA1 is a serine-type protease found in thylakoid membranes of photosynthetic organisms. This family is represented in higher plants by one component, in most cyanobacteria – by two proteins (SppA1 and SppA2). SppA1 is a light activated protease and may be involved in the acclimation of photosynthetic membranes to high light.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4 C, Long-term -20 . Repeated freezing and thawing is not recommended. It ontains 0,01% sodium azide.
Serine/threonine protein kinase (EC=2.7.11.1), known as STN8 in Arabidopsis, and its Stl1 homologue in Chlamydomonas, are STN7/Stt7-like proteins that are not required for state transitions. The Arabidopsis mutants deficient in stn8 gene demonstrated highly reduced levels of the phosphorylated proteins of the photosystem II (Bonardi et al., 2005 Varionen et al., 2005). Alternative name: Protein STATE TRANSITION 8.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Triticum aestivum
Expected Species:
Oryza sativa Species of your interest not listed? Contact us
Immunogen:
KLH-conugated synthetic peptide (amino acids 425 - 438) specific for Arabidopsis thaliana STN8 serine/threonine proteinkinase, UniProt: Q9LZV4 TAIR:At5g01920
For best results with this antibody sample buffer needs to contain 6 M urea (138mM TrisHCl pH 6.8, 6M urea, 22.2% Glycerol, 4.3% SDS).STN8 is a nuclear encoded protein which is localized in chloroplast, hence posses a chloroplastic target peptides (cTP) at the beginning of the amino acid sequence which is cut off. Accroding to TargetP (program that predicts the length of the cTP:s) the length of cTP for Stn8 is 49 amino acids. STN8 portein mobility can be also affected by urea present in the gel.Due to a high background signal with LHCII it is adviced to cut off a membrane below 30 kDa marker.
Application Details:
1 : 2000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
54,9 kDa or 46 kDa on 6 M urea gel
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Li et al. (2015). Effect of hydrogen sulfide on D1 protein in wheat under drought stress. Acta Physiologiae Plantarum November 2015, 37:225.Flood et al. (2014). Natural variation in phosphorylation of photosystem II proteins in Arabidopsis thaliana: is it caused by genetic variation in the STN kinases? Philos Trans R Soc Lond B Biol Sci. 2014 Mar 3;369(1640):20130499. doi: 10.1098/rstb.2013.0499. Print 2014.Yin et al. (2012). Photosystem II Function and Dynamics in Three Widely Used Arabidopsis thaliana Accessions. PLOS ONE, open access.
Special application note:
An extract from STN8 mutant needs to be used in pararel to determine specific band of STN8 protein on a western blot
HtrA belongs to a serine-type Deg peptidase family, widespread among bacteria and eukaryotes. This family includes three related members HtrA (DegP), HhoA (DegQ) and HhoB (DegS). HtrA is a highly hydrophilic protein and probably resides in a soluble compartment.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store short-term 4°Cand long term at -20 °C. Repeated freezing and thawing is not recommended.
Host Animal:
Rabbit
Species Reactivity:
Synechocystis sp. PCC 6803
Expected Species:
Anabaena sp. PCC 7120, Nostoc sp. Species of your interest not listed? Contact us
Immunogen:
Synthetic peptide (amino acids 159 – 172) specific for Synechocystis sp. PCC 6803 HtrA Slr1204
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4 C, Long-term -20 . Repeated freezing and thawing is not recommended. It ontains 0,01% sodium azide.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Galvis et al. (2020). H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in chloroplast ATP synthase mutants. Plant Physiol. pp.01561.2019. doi: 10.1104/pp.19.01561.Koochak et al. (2019). The structural and functional domains of plant thylakoid membranes. Plant J. 2019 Feb;97(3):412-429. doi: 10.1111/tpj.14127.Lv et al. (2019). Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. Plant Cell. 2019 Jan;31(1):210-230. doi: 10.1105/tpc.18.00813.Gao et al. (2018). A supercomplex, approximately 720 kDa and composed of both photosystem reaction centers, dissipates excess energy by PSI in green macroalgae under salt stress. Plant Cell Physiol. 2018 Oct 8. doi: 10.1093/pcp/pcy201.Koochak et al. (2018). The structural and functional domains of plant thylakoid membranes. Plant J. 2018 Oct 12. doi: 10.1111/tpj.14127. (BN-PAGE)Rantala and Tikkanen et al. (2018). Phosphorylation‐induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2.Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658. Grieco et al. (2015). Light-harvesting II antenna trimers connect energetically the entire photosynthetic machinery - including both photosystems II and I. Biochim Biophys Acta. 2015 Jun-Jul;1847(6-7):607-19. doi: 10.1016/j.bbabio.2015.03.004. Epub 2015 Apr 3.Yap at al. (2015). AEF1/MPR25 is implicated in RNA editing of plastid atpF and mitochondrial nad5 and also promotes atpF splicing in Arabidopsis and rice. Plant J. 2015 Jan 13. doi: 10.1111/tpj.12756.
Special application note:
This product can be sold containing proClin if requested
HliD (ScpE) protein is LHC-similar stress-induced protein from cyanobacteria. HliD is associated with damaged photosystem II and can serve as a temporary pigment reservoir while photosystem II components are being replaced.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store short-term 4°Cand long term at -20 °C. Repeated freezing and thawing is not recommended.
Host Animal:
Rabbit
Species Reactivity:
Synechocystis sp. PCC 6803
Expected Species:
According to sequence analysis antibody may react with homologous Hli protein(-s) from Anabaena, Thermosynechococcus, Gloeobacter, Prochlorococcus, Synechococcus and Crocosphaera.
Immunogen:
Synthetic peptide (amino acids 15 – 30) derived from Synechocystis sp. PCC 6803 HliD protein NP_440269.1
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Proctor et al. (2020) Xanthophyll carotenoids stabilise the association of cyanobacterial chlorophyll synthase with the LHC-like protein HliD. Biochem J. 2020 Oct 30;477(20):4021-4036. doi: 10.1042/BCJ20200561. PMID: 32990304.Chidgey et al. (2014). A cyanobacterial chlorophyll synthase-HliD complex associates with the Ycf39 protein and the YidC/Alb3 insertase. Plant Cell. 2014 Mar;26(3):1267-79. doi: 10.1105/tpc.114.124495.
Special application note:
Pre-immune serum is available to this product upon request
Enolase (2-phospho-D-glycerate hydrolase or phosphopyruvate dehydratase) is an essential glycolytic metalloenzyme. It is catalyzing the interconversion of 2-phosphoglycerate and phosphoenolpyruvate. Alternative name: Bifunctional enolase 2/transcriptional activator
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Helianthus annuus
Expected Species:
Brassica sp., Chlamydomonas reinhardii, Lycopersicum esculentum, Gossypium mexicanum, Nannochloropsis gaditana, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Populus balsamifera, Ricinus communis, Zea mays Species of your interest not listed? Contact us
Antibody is specific for the ENO2 isoform (cytosolic and active isoform), see data below
Application Details:
1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
47.7 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Zhang et al. (2020). A moonlighting role for enzymes of glycolysis in the co-localization of mitochondria and chloroplasts. Nat Commun. 2020 Sep 9;11(1):4509.doi: 10.1038/s41467-020-18234-w. Zhang et al. (2018). Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation. Plant Cell Environ. 2017 Sep;40(9):1834-1848. doi: 10.1111/pce.12989.Chen et al.(2009) System analysis of an Arabidopsis mutant altered in de novo fatty acid synthesis reveals diverse changes in seed composition and metabolic regulation. Plant Physiol.
Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Adhikari et al. (2018). Sulfate improves cadmium tolerance by limiting cadmium accumulation, modulation of sulfur metabolism and antioxidant defense system in maize. Environmental and Experimental Botany Volume 153, September 2018, Pages 143-162.Bastow et al. (2018). Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds. Plant Physiol. 2018 Jul;177(3):1267-1276. doi: 10.1104/pp.18.00478.Alch et al. (1998). Identification and immunolocalization of superoxide dismutase isoenzymes of olive pollen". Physiol. Plantarum 104, 772-776.
Special application note:
Total IgY concentration is 2.3 mg/ml.Reaction of the antibody to chloroplastic SOD isoform has not been determined yet.
Goat anti-guinea pig IgG is a secondary antibody conjugated to HRP which binds to all guinea pig IgGs in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Heavy chains on guinea pig IgG and light chains on all guinea pig immunoglobulins
Immunogen:
Purified Guinea pig IgG, whole molecule
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Not reactive in:
Non-immunoglobulin guinea pig serum proteins
Selected references:
Jin et al. (2019). YAP activation promotes the transdifferentiation of cardiac fibroblasts to myofibroblasts in matrix remodeling of dilated cardiomyopathy. Brazilian Journal of Medical and Biological Research (2019) 52(1): e7914.
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 10 % (w/v) BSA, Protease/IgG free with 0,1 % (v/v) of Kathon CG as preservative
Goat anti-guinea pig IgG is a secondary antibody conjugated to HRP which binds to all guinea pig IgGs in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Guinea pig IgG heavy and light chains (H&L) of all guinea pig immunoglobulins
Expected Species:
Guinea Pig IgG Heavy and Light chains (H&L) of all Guinea Pig immunoglobulins
Immunogen:
Purified Guinea pig IgG, whole molecule
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to non-immunoglobulin guinea pig serum proteins, No reactivity is observed to serum from bovine, hen, goat, hamster, horse, human, mouse, rabbit, rat or sheep based on immunoelecrophoresis
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative.
No reactivity is observed to non-immunoglobulin guinea pig serum proteins based in immunoelectrophoresis.This antibody reacts with the heavy chains on guinea pig IgG and with the light chains on all guinea pig immunoglobulins based on immunoelectrophoresis.
Goat anti-guinea pig IgG is a secondary antibody conjugated to ALP (alkaline phosphatase) which binds to all guinea pig immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilution prior to use and then discard. Shelf life of this product is one year from date of receipt.
Host Animal:
Goat
Expected Species:
Guinea Pig IgG Heavy and Light chains (H&L)
Immunogen:
Purified Guinea pig IgG, whole molecule
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to non-immunoglobulin guinea pig serum proteins based in immunoelectrophoresis. This antibody reacts with the heavy chains on guinea pig IgG and with the light chains on all guinea pig immunoglobulins based on immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Rabbit anti-goat IgG is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all donkey immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Rabbit
Species Reactivity:
Goat IgG heavy and light chains (H&L)
Expected Species:
Goat IgG Heavy and Light chains (H&L)
Immunogen:
Purified Goat IgG, whole molecule
Applications:
ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to non-immunoglobulin goat serum proteins based or IgG from human, mouse or rat based on immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
APL conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
No reactivity is observed to non-immunoglobulin goat serum proteins based in immunoelectrophoresis.No reactivity is observed to human, mouse and rat IgG based on immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservativeAntibody binds to heavy chains of goat IgG and light chains of all goat immunoglobulins based on immunoelectrophoresis,?BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG
Rabbit anti-goat IgG is a secondary antibody conjugated to HRP which binds to goat IgGs in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Rabbit
Species Reactivity:
Goat IgG heavy chains and light chains on all Goat immunoglobulins
Expected Species:
Goat IgG Heavy chains and Light chains on all Goat immunoglobulins
Immunogen:
Purified Goat IgG, whole molecule
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to non-immunoglobulin goat serum proteins. No reactivity is observed to serum from human, mouse and rat based on immunoelecrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative.
Goat anti-human IgA+IgG+IgM is a secondary antibody conjugated to HRP which binds to human immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgA+IgG+IgM heavy chains and light chains on all Human immunoglobulins
Expected Species:
Human IgA+IgG+IgM Heavy chains and Light chains on all Human immunoglobulins
Immunogen:
affinity purified human IgA+IgG+IgM
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative.
Goat anti-rat IgG is a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all donkey immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Heavy chains on Rat IgG and with the light chains on all Rat immunoglobulins based on IEP
Expected Species:
Heavy chains on Rat IgG and with the Light chains on all Rat immunoglobulins based on IEP
Immunogen:
Purified Rat IgG, whole molecule
Applications:
ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody is highly cross absorbed against mouse IgG, No reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis, No reactivity is observed to human or mouse IgG based on immunoelecrophoresis,
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Li et al. (2022), The effects of Ni availability on H2 production and N2 fixation in a model unicellular diazotroph: The expression of hydrogenase and nitrogenase. Limnol Oceanogr, 67: 1566-1576. https://doi.org/10.1002/lno.12151
Special application note:
APL conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Goat anti-human IgG Fc is a secondary antibody conjugated to HRP which binds to human Fc part of IgG in immunological assays. The antibody is absorbed agains human IgA and IgM
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG heavy chain Fc based on immunoelectrophoresis
Expected Species:
Human IgG Fc
Immunogen:
affinity purified human IgG Fc part
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative.
No reactivity is observed to non-immunoglobulin proteins from rabbit serum
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative. Clear, colorless liquid, 0.2 m filtered. ≥ 95 % purity based on SDS-PAGE.
Goat anti-rabbit IgG is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Rabbit IgG heavy and light chains (H&L)
Expected Species:
Rabbit IgG Heavy and Light chains (H&L)
Immunogen:
Purified Rabbit IgG, whole molecule (H&L chains)
Applications:
Immunohistochemistry (IHC), ELISA (ELISA), Western blot (WB)
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
No reactivity is observed to bovine/goat/human/mouse or rat IgG based on immunoelectrophoresis.No reactivity to non-immunoglobulin rabbit serum proteins.Antibody is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1 % (v/v)Kathon CG is added as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.
Goat anti-rabbit IgG is a secondary antibody conjugated to HRP which binds to rabbit IgGs in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
heavy chains of Rabbit IgG, Light chains on all Rabbit immunoglobulins
Expected Species:
Rabbit IgG Heavy and Light chains (H&L) of all Rabbit immunoglobulins
Immunogen:
purified Rabbit IgG, whole molecule
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
Based on immunoelectrophoresis no reactivity is observed to:non-immunoglobulin rabbit serum proteinsserum from bovine, human or mouseIgG from human or mouse
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Lacour et al. (2019). Decoupling light harvesting, electron transport and carbon fixation during prolonged darkness supports rapid recovery upon re-illumination in the Arctic diatom Chaetoceros neogracilis. Polar Biol (2019). https://doi.org/10.1007/s00300-019-02507-2.
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)
AGO5 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). Probably involved in antiviral RNA silencing.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
peptide of Arabidopsis thaliana AGO5 UniProt: Q9SJK3, TAIR:AT2G27880
Applications:
Immunofluorescebce (IF), Immunoprecipitation (IP), Western blot (WB)
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest, Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure
Application Details:
1 : 100 (IF), 5 ug per gram floral tissue (IP), 1 : 1500 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
111 | 111 kDa
Not reactive in:
Hordeum vulgare, Solanum lycopersicum, Zea mays
Selected references:
Oliver & Martinez. (2021) Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis. Plant Reprod. 2021 Nov 23. doi: 10.1007/s00497-021-00434-z. Epub ahead of print. PMID: 34812935.
AGO6 (Argonaute 6) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana AGO6 UniProt: O48771 TAIR:At2g32940
AGO6 expression is cell specific, Seedlings can be used as a negative control
Application Details:
1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
116,4 | 99 kDa
Not reactive in:
Zea mays
Selected references:
Oliver & Martinez. (2021) Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis. Plant Reprod. 2021 Nov 23. doi: 10.1007/s00497-021-00434-z. Epub ahead of print. PMID: 34812935.Sprunck et al. (2019). Elucidating small RNA pathways in Arabidopsis thaliana egg cells. http://dx.doi.org/10.1101/525956 Havecker el al. (2010). The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci. Plant Cell. 2010 Feb;22(2):321-34. doi: 10.1105/tpc.109.072199.
Special application note:
There can be a reaction with NEB broad range prestained protein ladder depending upon secondary antibody used.AGO6 accumulates to lower levels in the nrpd1a mutant (Havecker at el. 2010). In addition, a non-specific band migrates to a similar location and thus protein was run on a 6% denaturing gel to obtain good separation.If other secondary antibody is used than in the figure below, cross-reacting pattern can be different.
AGO9 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AGO9 protein sequence UniProt: Q84YI4, TAIR:At5g21150.
Applications:
Immunolocalization, whole-mount (IL), Immunoprecipitation (IP), Western blot (WB)
AGO expression may be cell/tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Seedlings can be used as a negative control. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.A recommended whole-mount immunolocalization protocol can be found here.
Application Details:
5 g of antibody per 1 gram of a fresh tissue (IP), 1 : 10 000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
101 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Hou et al. (2021) High-throughput single-cell transcriptomics reveals the female germline differentiation trajectory in Arabidopsis thaliana. Commun Biol. 2021 Oct 1;4(1):1149. doi: 10.1038/s42003-021-02676-z. PMID: 34599277; PMCID: PMC8486858. (immunolocalization)Oliver & Martinez. (2021) Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis. Plant Reprod. 2021 Nov 23. doi: 10.1007/s00497-021-00434-z. Epub ahead of print. PMID: 34812935.Sprunck et al. (2019). Elucidating small RNA pathways in Arabidopsis thaliana egg cells. http://dx.doi.org/10.1101/525956Su et al. (2017). The THO Complex Non-Cell-Autonomously Represses Female Germline Specification through the TAS3-ARF3 Module. Curr Biol. 2017 Jun 5;27(11):1597-1609.e2. doi: 10.1016/j.cub.2017.05.021.Havecker et al. (2010) The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci. Plant Cell 22(2): 321-34.
eEF1B-gamma1 protein belongs to a family of elongation factors, proteins which are involved in translational elongation. Alternative names: EF-1-gamma 1, eEF-1B gamma 1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Oryza sativa, Solanum tuberosum, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
Recombinant eEF1B-gamma1 protein from Arabidopsis thaliana with no affinity tag,UniProt: O04487, TAIR: At1g09640
No confirmed exceptions from predicted reactivity are currently known
Selected references:
McLoughlin et al. (2019) HSP101 Interacts with the Proteasome and Promotes the Clearance of Ubiquitylated Protein Aggregates. Plant Physiol. 2019 Aug;180(4):1829-1847. doi: 10.1104/pp.19.00263McLoughlin et al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.
Special application note:
Antibody is recognizing elongation factor 1-gamma 1 and gamma 2
eEF1B-beta1 protein belongs to a family of elongation factors, proteins which are involved in translational elongation. Alternative names: Elongation factor 1-delta 1, EF-1-delta 1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Oryza sativa, Ricinus communis, Solanum tuberosum, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
recombinant eEF1B-beta1 protein from Arabidopsis thaliana with no affinity tag, P48006, At1g30230
No confirmed exceptions from predicted reactivity are currently known
Selected references:
McLoughlin et al. (2019) HSP101 Interacts with the Proteasome and Promotes the Clearance of Ubiquitylated Protein Aggregates. Plant Physiol. 2019 Aug;180(4):1829-1847. doi: 10.1104/pp.19.00263McLoughlin et al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.
Special application note:
Antibody is recognizing both proteins: elonagtion factor beta 1 and beta 2
eEF1B-alpha1 protein belongs to a family of elongation factors, proteins which are involved in translational elongation. This protein is a component of elongation factor B-complex which is composed of 2 elongation B-alpha-(1 and 2), 2 elongation factors B-beta (1 and 2) and 2 elongation factors B-gamma (1 and 2). Alternative names:
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Glycne max, Oryza sativa, Solanum tuberosum, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
Recombinant eEF1B-alpha1 protein from Arabidopsis thaliana with no affinity tag,UniProt: Q84WM9, TAIR: At5g12110
eEF1B-alpha2 protein belongs to a family of elongation factors, proteins which are involved in translational elongation. Alternative names: Elongation factor 1-beta 2, Elongation factor 1-beta' 2, EF-1-beta' 2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Glycne max, Oryza sativa, Solanum tuberosum, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
Recombinant eEF1B-alpha2 protein from Arabidopsis thaliana with no affinity tag, UniProt: Q9SCX3,TAIR: At5g19510
No confirmed exceptions from predicted reactivity are currently known
Selected references:
McLoughlin et al. (2019) HSP101 Interacts with the Proteasome and Promotes the Clearance of Ubiquitylated Protein Aggregates. Plant Physiol. 2019 Aug;180(4):1829-1847. doi: 10.1104/pp.19.00263McLoughlin et al. (2016) Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress. Plant Physiol. 2016 Oct;172(2):1221-1236.
Special application note:
Antibody shows a very weak cross-reaction to elongation factor 1B-alpha 1
Tubulin alpha (TUA) together with beta tubulin is making up microtubules.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Brassica napus, Chlorella vulgaris, Chlorella variabilis, Cucumis sativus, Euglena gracilis, Glycine max, Micromonoas pusilla, Nannochloropsis gaditana, Ostreococcus lucimarinus, Pisum sativum, Physcomitrium patens, Picea sitchensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Triticum aestivum, Vigna radiata, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from available tubulin alpha chain sequences including Arabidopsis thaliana tubulin alpha-1-chain P11139(At1g64740), alpha-2/alpha-4 chain B9DGT7(At1g50010), alpha-5 chain B9DHQ0(At5g19780), alpha-6-chain P29511(At4g14960)Peptide used to elict this antibody is not present in tubulin beta.
Metal induced stress affected the expression of tubulin, and that therefore, this protein cannot be used as a loading control under that type of conditions. More information can be found here.
Application Details:
1 : 500 (IF), 1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
49 | 52 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Kumar et al. (2022). Proteomic dissection of rice cytoskeleton reveals the dominance of microtubule and microfilament proteins, and novel components in the cytoskeleton-bound polysome, Plant Physiology and Biochemistry, Volume 170,2022,Pages 75-86,ISSN 0981-9428, https://doi.org/10.1016/j.plaphy.2021.11.037.Gu et al. (2021) A Lipid Bodies-Associated Galactosyl Hydrolase Is Involved in Triacylglycerol Biosynthesis and Galactolipid Turnover in the Unicellular Green Alga Chlamydomonas reinhardtiiSakuraba at al. (2020). Multilayered regulation of membrane-bound ONAC054 is essential for abscisic acid-induced leaf senescence in rice. Plant Cell. 2020 Jan 6. pii: tpc.00569.2019. doi: 10.1105/tpc.19.00569.Roustan et al. (2020). Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s. Sci Rep. 2020 Feb 5;10(1):1864. doi: 10.1038/s41598-020-58740-x.Roustan et al. (2020). Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s. Sci Rep. 2020 Feb 5;10(1):1864. doi: 10.1038/s41598-020-58740-x.
Tubulin beta (TUB) together with alpha tubulin is making up microtubules.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Metal induced stress affected the expression of tubulin, and that therefore, this protein cannot be used as a loading control under that type of conditions. More information can be found here.
Application Details:
1 : 1000 (IF), 1 : 500 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
49 | 49 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Durian et al. (2019). PROTEIN PHOSPHATASE 2A-B' gamma controls Botrytis cinerea resistance and developmental leaf senescence. Plant Physiol. 2019 Oct 28. pii: pp.00893.2019. doi: 10.1104/pp.19.00893.Wang et al. (2017). The inhibition of protein translation mediated by AtGCN1 is essential for cold tolerance in Arabidopsis thaliana. Plant Cell Environ. 2017 Jan;40(1):56-68. doi: 10.1111/pce.12826.Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113
Special application note:
Please, note that tubulin beta is only detected in actively dividing meristematic cells (see immunolocalization image below). Therefore to allow detection on a western blot, analyzed material must contain enough meristematic cells with dividing activity.Signal in a western blot application in Chlamydomonas reinhardtii is obtained with a load of 100 g/well and a dilution of 1: 500.
PsbP-like protein (sll1418) is a cyanobacterial homologue of plant PsbP-like protein. It is localized in the thylakoid membrane and associated with photosystem II. Synonymes: Sll1418 protein
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Synechocystis sp. PCC 6803
Expected Species:
Anabaena variabilis, Arthrospira maxima, Lyngbya sp. PCC 8106, Microcoleus chthonoplastes, Ostreococcus lucimarinus, Trichodesmium erythraeum, Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from PsbP -like protein of Synechocystis sp. PCC 6803, UniProt: P73952
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526.Sveshnikov et al. (2007) The PsbP-like protein (sll1418) of Synechocystis sp. PCC 6803 stabilises the donor side of Photosystem II, Photosynth. Res. 93, 101-109.Ishikawa et al. . (2005) Functional analysis of the PsbP-like protein (sll1418) in Synechocystis sp. PCC 6803, Photosynth. Res. 84, 257-262.
HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C for 1 year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Antibody in concentration 1 g/ml was sufficient for detection of HDEL-containing proteins in 10 g of yeast cell lysate by colorimetric western blot. Clone 2E7. For western blot results, immunofluorescence and immunogold images please referr to Napier et al. 1992.
Application Details:
1: 50-1 : 500 (IF), 1 : 100-1, 1000 (WB)
Conjugation:
IgG2B
Isotype:
IgG2B
Purity:
Total IgG. Protein G purified in PBS pH 7.4.
Molecular Weight:
78 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Luo et al. (2006). GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol Cell Biol. 26(15): 5688-5697.Napier et al. (1992). Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. J Cell Sci. 102: 261-271.
Special application note:
Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/ml
HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C for 1 year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Antibody in concentration 1 g/ml was sufficient for detection of HDEL-containing proteins in 10 g of yeast cell lysate by colorimetric western blot. Clone 2E7.For western blot results, immunofluorescence and immunogold images please referr to Napier et al. 1992.
Application Details:
1, 50-1 : 500 (IF), 1 : 100-1, 1000 (WB)
Conjugation:
IgG2B
Isotype:
IgG2B
Purity:
Total IgG. Protein G purified in PBS pH 7.4.
Molecular Weight:
78 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Ming-fang et al. (2021) Improved quantification of immune-gold labeling and its use to compare the distribution of cellular factors among sub-chloroplast compartments,Micron,2021,103060,ISSN 0968-4328,https://doi.org/10.1016/j.micron.2021.103060.Luo et al. (2006). GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol Cell Biol. 26(15): 5688-5697.Napier et al. (1992). Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. J Cell Sci. 102: 261-271.
Special application note:
Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/ml
Alcohol dehydrogenase (ADH) is an enzyme playing a crucial role in the fermentative metabolism in plants subjected to low oxygen stress. It is known to be synthesized preferentially under low oxygen conditions.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Allyl alcohol dehydrogenase of Nicotiana tabacum, accession 75206691 and in Chlamydomonas reinhardtii.
Selected references:
Czernicka et al. (2022). Proteomic Studies of Roots in Hypoxia-Sensitive and -Tolerant Tomato Accessions Reveal Candidate Proteins Associated with Stress Priming. Cells. 2022 Jan 31;11(3):500. doi: 10.3390/cells11030500. PMID: 35159309; PMCID: PMC8834170.Ventura et al. (2020). Arabidopsis phenotyping reveals the importance of alcohol dehydrogenase and pyruvate decarboxylase for aerobic plant growth. Sci Rep. 2020 Oct 7;10(1):16669. doi: 10.1038/s41598-020-73704-x. PMID: 33028901; PMCID: PMC7542448.Gil-Monreal et al. (2019). ERF-VII transcription factors induce ethanol fermentation in response to amino acid biosynthesis-inhibiting herbicides. J Exp Bot. 2019 Aug 6. pii: erz355. doi: 10.1093/jxb/erz355.Bui et al. (2019). Conservation of ethanol fermentation and its regulation in land plants. J Exp Bot. 2019 Feb 28. pii: erz052. doi: 10.1093/jxb/erz052.De la Rosa et al. (2019), A dicistronic precursor encoding miR398 and the legume-specific miR2119 coregulates CSD1 and ADH1 mRNAs in response to water deficit. Plant Cell Environ. 2019 Jan;42(1):133-144. doi: 10.1111/pce.13209.
Special application note:
This product can be sold containing ProClin if requested
Pathogenesis-related protein 1 (PR-1), small antimicrobial protein which acts as a marker of plant immune signaling and is partially responsible for acquired pathogen resistance. Induced by INA, salicylic acid and pathogen infection.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Re-using of antibody solution is not recommended, It will contribute to incrteased background signal
Application Details:
1 : 2500 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
17.7 kDa (Arabidopsis thaliana)
Not reactive in:
Citrus sinensis,
Selected references:
Li et al. (2020). N-terminal acetylation stabilizes SIGMA FACTOR BINDING PROTEIN 1 involved in salicylic acid-primed cell death. Plant Physiol. 2020 Mar 5. pii: pp.01417.2019. doi: 10.1104/pp.19.01417.Jung et al. (2020). Pathogen-associated Molecular Pattern-triggered Immunity Involves Proteolytic Degradation of Core Nonsense-mediated mRNA Decay Factors During the Early Defense Response. Plant Cell, February 2020. Chang et al. (2019). PBS3 Protects EDS1 from Proteasome-Mediated Degradation in Plant Immunity. Mol Plant. 2019 Feb 11. pii: S1674-2052(19)30055-3. doi: 10.1016/j.molp.2019.01.023.Lv et al. (2019). Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. Plant Cell. 2019 Jan;31(1):210-230. doi: 10.1105/tpc.18.00813.Cecchini et al. (2018). Underground azelaic acid-conferred resistance to Pseudomonas syringae in Arabidopsis. Mol Plant Microbe Interact. 2018 Aug 29. doi: 10.1094/MPMI-07-18-0185-R.Chakraborty et al. (2018). Epigenetic and transcriptional control of chickpea WRKY40 promoter activity under Fusarium stress and its heterologous expression in Arabidopsis leads to enhanced resistance against bacterial pathogen. Plant Science, doi.org/10.1016/j.plantsci.2018.07.014Izquierdo et al. (2018). Arabidopsis nonresponding to oxylipins locus NOXY7 encodes a yeast GCN1 homolog that mediates noncanonical translation regulation and stress adaptation. Plant Cell Environ. 2018 Mar 2. doi: 10.1111/pce.13182.Seguel et al. (2018). PROHIBITIN 3 forms complexes with ISOCHORISMATE SYNTHASE 1 to regulate stress-induced salicylic acid biosynthesis in Arabidopsis. Plant Physiol. Jan 2018. DOI:10.1104/pp.17.00941Huh et al. (2017). Protein-protein interactions in the RPS4/RRS1 immune receptor complex. PLoS Pathog. 2017 May 5;13(5):e1006376. doi: 10.1371/journal.ppat.1006376.Zhang et al. (2017). A suite of receptor-like kinases and a putative mechano-sensitive channel are involved in autoimmunity and plasma membrane-based defenses in Arabidopsis. Mol Plant Microbe Interact. 2017 Jan 4. doi: 10.1094/MPMI-09-16-0184-R.Zhu et al. (2016). CML8, an Arabidopsis calmodulin-like protein plays a role in Pseudomonas syringae plant immunity. Plant Cell Physiol. 2016 Nov 10. pii: pcw189. [Epub ahead of print]
Special application note:
PR-1 protein is present in very low amonts in non-induced plant material.Overnight antibody incubation is not recommended.This product can be sold containing Proclin if requested.
Pathogenesis-related protein 1 (PR-1) is partially responsible for acquired pathogen resistance. Induced by INA, salicylic acid and pathogen infection.This product is a recombinant PR-1 protein, trunctated by first 26 amino acids, source: Arabidopsis thaliana, UniProt: P33154, TAIR: At2g14610
Product Type:
Antibody
Format:
Lyophilized in glycerol.
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Concentration: after adding 90 l of sterile milliQ water final concentration of the standard is 0.10 pmoles/ lProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:
Standard curve: 3 loads are recommended eg.0.5, 2 and 4μl.For most applications a sample load of 10-20 μg of protein will provide with a signal in this range.Positive control:a 2μl load per well is optimal for most chemiluminescent detection systems.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:
For reconstitution add 90 l of sterile water, Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized
Molecular Weight:
16,4 kDa
Special application note:
The PR-1 protein standard can be used in combination with anti-PR-1 antibodies to quantitate PR-1 protein. Quantitative western blot: detailed method description, video tutorialThis product can be sold containing ProClin if requested
HCP (hyper conserved protein) is perfectly conserved between Prochlorococcus and Synechococcus group. So far its exact function is not known.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Prochlorococcus MIT 9313
Expected Species:
Cyanobacteria Species of your interest not listed? Contact us
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Whidden et al. (2014). Quantitative and functional characterization of the hyper-conserved protein of prochlorococcus and marine synechococcus. PLoS One. 2014 Oct 31;9(10):e109327. doi: 10.1371/journal.pone.0109327. eCollection 2014.
GluTR (glutamyl tRNA reductase) belongs to a family of oxidoreductases and is involved in porphyrin and chlorophyll biosynthesis. This enzyme class is called L-glutamate-semialdehyde: NADP+ oxidoreductase (L-glutamyl-tRNAGlu-forming).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Agrawal et al. (2020). The Functions of Chloroplast Glutamyl-tRNA in Translation and Tetrapyrrole Biosynthesis. Plant Physiol. 2020 Feb 18. pii: pp.00009.2020. doi: 10.1104/pp.20.00009Montandon et al. (2019). In vivo trapping of proteins interacting with the chloroplast CLPC1 chaperone; potential substrates and adaptors. J Proteome Res. 2019 May 9. doi: 10.1021/acs.jproteome.9b00112.Nishimura et al. (2013). ClpS1 Is a Conserved Substrate Selector for the Chloroplast Clp Protease System in Arabidopsis. The Plant Cell June 2013.
Special application note:
Antibody reacts with recombinant GluTR isoforms: AtGluTR1, AtGluTR2 and NtGluTR1 (At - Arabidopsis thaliana, Nt - Nicotiana tabacum).
Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Amborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandis, Glycine max, Chlorella variabilis, Leucaena glauca, Lotus japonicus, Medicago tribuloides, Mimulus guttatus, Musa malaccensis, Oryza sativa, Panicum italicum, Physcomitrium patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Populus trichocarpa, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays.Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530
Applications:
Immunolocalization (IL), Immunofluorescence (IF), Western blot (WB)
Please, note that fresh samples will provide better restuls (see image below)
Application Details:
1 : 2400 (IL), 1 :400 (IF), 1 : 2000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
193 | 170 kDa (Arabidopsis thaliana)
Not reactive in:
Nicotiana benthamiana
Selected references:
Fujimoto et al. (2020) Longin R-SNARE is retrieved from the plasma membrane by ANTH domain-containing proteins in Arabidopsis. Proc Natl Acad Sci U S A. 2020 Oct 6;117(40):25150-25158. doi: 10.1073/pnas.2011152117. Epub 2020 Sep 23. PMID: 32968023; PMCID: PMC7547277.Ranjan et al. (2019). Transient Internalization and Microtubule-Dependent Trafficking of a Ciliary Signaling Receptor from the Plasma Membrane to the Cilium. Curr Biol. 2019 Aug 2. pii: S0960-9822(19)30867-X. doi: 10.1016/j.cub.2019.07.022.Wattelet-Boyer et al. (2016). Enrichment of hydroxylated C24- and C26-acyl- chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains. Nat Commun. 2016 Sep 29;7:12788. doi: 10.1038/ncomms12788.Derbyshire et al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314.Grones et al. (2015). Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. J Exp Bot. 2015 Apr 28. pii: erv177.
Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Amborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandis, Glycine max, Chlorella variabilis, Leucaena glauca, Lotus japonicus, Medicago tribuloides, Mimulus guttatus, Musa malaccensis, Oryza sativa, Panicum italicum, Physcomitrium patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530
Applications:
Immunolocalization (IL), Immunofluorescence (IF), Western blot (WB)
Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Amborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandis, Glycine max, Chlorella variabilis, Leucaena glauca, Lotus japonicus, Medicago tribuloides, Mimulus guttatus, Musa malaccensis, Oryza sativa, Panicum italicum, Physcomitrium patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530
Applications:
Immunolocalization (IL), Immunofluorescence (IF), Western blot (WB)
Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles, It consists of three clathrin heavy chains and three light chains, Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Amborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandis, Glycine max, Chlorella variabilis, Leucaena glauca, Lotus japonicus, Medicago tribuloides, Mimulus guttatus, Musa malaccensis, Oryza sativa, Panicum italicum, Physcomitrium patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Populus trichocarpa, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays.Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530
Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles, It consists of three clathrin heavy chains and three light chains, Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
Zea mays
Expected Species:
Amborella trichopoda, Arabidopsis thaliana, Brassica napus, Capsella rubella, Chlamydomonas reinhardtii, Citrus aurantium var. sinensis, Eucalyptus grandis, Glycine max, Chlorella variabilis, Leucaena glauca, Lotus japonicus, Medicago tribuloides, Mimulus guttatus, Musa malaccensis, Nicotiana tabacum, Oryza sativa, Panicum italicum, Physcomitrium patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Populus trichocarpa, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays.Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530
Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles, It consists of three clathrin heavy chains and three light chains, Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Amborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandis, Glycine max, Chlorella variabilis, Leucaena glauca, Lotus japonicus, Medicago tribuloides, Mimulus guttatus, Musa malaccensis, Oryza sativa, Panicum italicum, Physcomitrium patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Populus trichocarpa, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays.Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530
Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Amborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandis, Glycine max, Chlorella variabilis, Leucaena glauca, Lotus japonicus, Medicago tribuloides, Mimulus guttatus, Musa malaccensis, Oryza sativa, Panicum italicum, Physcomitrium patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530
Applications:
Immunolocalization (IL), Immunofluorescence (IF), Western blot (WB)
Pyruvate decarboxylase (PDC) is a homotetrameric enzyme (E.C.4.1.1.1) that catalyses the decarboxylation of pyruvic acid to acetaldehyde carbon dioxide in the cytoplasm. It is also called 2-oxo-acid carboxylase, and pyruvic decarboxylase. In anaerobic conditions, this enzyme is part of the fermentation process that occurs in yeast, especially the Saccharomyces genus, to produce ethanol by fermentation. Pyruvate decarboxylase starts this process by converting pyruvate into acetaldehyde and carbon dioxide. Pyruvate decarboxylase depends on cofactors thiamine pyrophosphate (TPP) and magnesium. This enzyme should not be mistaken for the unrelated enzyme pyruvate dehydrogenase, an oxidoreductase (EC 1.2.4.1), that catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
The PsbI protein, previously named the 4.8-kDa protein, is encoded by the plastome. PsbI is a universal component of PSII and is highly conserved (e.g. there is 71% amino acid identicality between the Arabidopsis and Synechocystis 6803 proteins). The protein contains 36 to 38 amino acids in most species, with molecular masses ranging between 4.1 and 4.5 kDa. Synonymes: PSII-I, PSII 4.8 kDa protein
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Synechocystis sp. PCC 6803
Expected Species:
Cyanobacteria Species of your interest not listed? Contact us
Loads higher than 0,5 g of chlorophyll per well are not recommended
Application Details:
1 : 5000-1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
4.3 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Dobakova et. al (2007). Role of the PsbI Protein inPhotosystem II Assembly and Repair in the Cyanobacterium Synechocystis sp. PCC 6803 Plant Physiol 145:1681-1691.
Photosystem I (PSI) of chloroplasts is a multisubunit membrane-protein complex that catalyzes the electron transfer from the reduced plastocyanin (or cytochrome c6) in the thylakoid lumen to the oxidized ferredoxin (or flavodoxin) in the chloroplast stroma. PsaB is a core protein of PSI complex. Synonymes: Photosystem I P700 chlorophyll a apoprotein A2, PSI-B
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This product can be sold containing ProClin if requested
Application Details:
1 : 1000 (BN-PAGE), (WB)
Purity:
Antigen affinity purified in PBS pH 7.4
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
82,7 | 55-60 kDa
Not reactive in:
Chlamydomonas reinhardtii, dinoflagellate
Selected references:
Shukla et al. (2020). A novel method produces native LHCII aggregates from the photosynthetic membrane revealing their role in non-photochemical quenching. J Biol Chem. 2020 Oct 20:jbc.RA120.016181. doi: 10.1074/jbc.RA120.016181. Epub ahead of print. PMID: 33082138.Grieco et al. (2020). Adjustment of photosynthetic activity to drought and fluctuating light in wheat. Plant Cell Environ. 2020 Mar 16. doi: 10.1111/pce.13756. Liu et al. (2020). Acid treatment combined with high light leads to increased removal efficiency of Ulva prolifera. Algal Research,Volume 45, January 2020, 101745Frede et al. (2019). Light quality-induced changes of carotenoid composition in pak choi Brassica rapa ssp. chinensis. J Photochem Photobiol B. 2019 Apr;193:18-30. doi: 10.1016/j.jphotobiol.2019.02.001.Lima-Melo et al. (2019). Consequences of photosystem-I damage and repair on photosynthesis and carbon use in Arabidopsis thaliana. Plant J. 2018 Nov 29. doi: 10.1111/tpj.14177.
RA - Ribulose bisphosphate carboxylase/oxygenase activase is an enzyme localized to chloroplasts which activates Rubisco by promoting ATP-dependent conformational changes. Alternative name: RuBisCo activase RCA.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
There are two forms of activase (alpha and beta) in some species (for example Arabidopsis, camelina, spinach, rice) and only one form in other species (tobacco, maize, Chlamydomonas). Alpha is about 46-47 Kda, beta is about 42 kDa. Species that have only one form have the beta form.This product can be sold containing ProClin if requested
Application Details:
1 : 5000-1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
47 and 42 kDa (maize, tobacco, Chlamydomonas)
Not reactive in:
marine picocyanobacteria
Selected references:
Amiya et al. (2021) Membrane DnaJ-Like Chaperone with Oxidizing Activity in Chlamydomonas reinhardtii. Int J Mol Sci. 2021 Jan 24;22(3):1136. doi: 10.3390/ijms22031136. PMID: 33498879; PMCID: PMC7865324.Oikawa et al. (2021) Mitochondrial movement during its association with chloroplasts in Arabidopsis thaliana. Commun Biol. 2021 Mar 5;4(1):292. doi: 10.1038/s42003-021-01833-8. PMID: 33674706.Wang et al. (2021) Insights Into the Gene Regulation in Jasmonate-Induced Whole-Plant Senescence of Tobacco Under Non-Starvation Condition. Plant Cell Physiol. 2021 Sep 15:pcab140. doi: 10.1093/pcp/pcab140. Epub ahead of print. PMID: 34523687.Trojak et al. (2021) Effects of partial replacement of red by green light in the growth spectrum on photomorphogenesis and photosynthesis in tomato plants. Photosynth Res. 2021 Sep 27. doi: 10.1007/s11120-021-00879-3. Epub ahead of print. PMID: 34580802.Yokochi et al.(2021) Oxidative regulation of chloroplast enzymes by thioredoxin and thioredoxin-like proteins in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 2021 Dec 21;118(51):e2114952118. doi: 10.1073/pnas.2114952118. PMID: 34907017; PMCID: PMC8713810.
Special application note:
This product can be sold containing ProClin if requested
Deg5 belongs to S1 chymotrypsin protease family and contains 16 protein members in Arabidopsis thaliana. Deg5 is localized to chloroplast, within the thylakoid. Alternative protein names: DegP5 or HhoA homologue or DegQ (in bacteria)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Malno et al. (2014). Thylakoid FtsH Protease Contributes to Photosystem II and Cytochrome b6f Remodeling in Chlamydomonas reinhardtii under Stress Conditions. Plant Cell, Jan 21.
The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Cucumis sativus, Glycine max, Nannochloropsis sp., Oryza sativa, Populus balsamifera, Ricinus communis, Zea mays, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide, amino acids 234-242 of Arabidopsis thaliana D1 protein UniProt: P83755, TAIR:AtCg00020
Antibody is recognizing a 23 kDa fragment in spinach and Arabidopsis thylakoidsfor usage on total cell extracts the dilution needs to be determined experimentally
Application Details:
1 : 10 000, thylakoid fraction (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
38 | 28-30 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Lu et al. (2021). Role of an ancient light-harvesting protein of PSI in light absorption and photoprotection. Nat Commun. 2021 Jan 29;12(1):679. doi: 10.1038/s41467-021-20967-1. PMID: 33514722; PMCID: PMC7846763. (blue-native PAGE)Mazur et al. (2021) The SnRK2.10 kinase mitigates the adverse effects of salinity by protecting photosynthetic machinery. Plant Physiol. 2021 Dec 4;187(4):2785-2802. doi: 10.1093/plphys/kiab438. PMID: 34632500; PMCID: PMC8644180.Rantala et al. (2020). PGR5 and NDH-1 systems do not function as protective electron acceptors but mitigate the consequences of PSI inhibition. Biochim Biophys Acta Bioenerg. 2020 Jan 11;1861(3):148154. doi: 10.1016/j.bbabio.2020.148154.Grieco et al. (2020). Adjustment of photosynthetic activity to drought and fluctuating light in wheat. Plant Cell Environ. 2020 Mar 16. doi: 10.1111/pce.13756. Rantala and Tikkanen et al. (2018). Phosphorylation?induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2.
Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine antibodies are helpful in facilitating the identification of tyrosine kinase substrates.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C for one year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Antibody reacts with phosphotyrosine and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysated, Does not cross react with phosphoserine or phosphothreonine
Immunogen:
Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin KLH with 1-ethyl-3-(3’-dimentrylaminopropyl) carbodiimide
Applications:
Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
1 g/ml of this antibody is sufficient for detection of phosphorylated tyrosine residues in 10 g of rat tissue lysate by colorimetric immunoblot analysis
Application Details:
1 : 1000 (WB)
Conjugation:
IgG1
Isotype:
IgG1
Purity:
Total IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Garton & Tonks (1999). Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST. J Biol Chem 6:3811-3818.Tiganis et al. (1999). The protein-tyrosine phosphatase TCPTP regulates epidermal growth factor receptor-mediated and phosphatidylinositol 3-kinase-dependent signaling. J Biol Chem 39: 27768-27775.(IF):Garton et al. (1996). Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST. Mol and Cell Bio 11:6408-6418.(IP):
Special application note:
Protein G purified IgG1 in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/ml
Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species. Protein tyrosine nitration results in a post-translational modification, component of nitric oxide signaling.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivitywith non-nitrated tyrosine. Not species specific.0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot..ECL.Antibody works paraffin-embedded sections.
Application Details:
1: 100 (IHC), 1: 1400 (WB), The exact and optimal working dilution should be determined by the investigator
Conjugation:
IgG2A
Isotype:
IgG2A
Purity:
Total IgG. Protein G purified, in PBS. Contains 50 % glycerol and 0.09% sodium azide.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.Antibody used in immunohistochemistry:Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387.
Special application note:
1 mg/ml of Protein G purified IgG2A in PBS pH 7,4, 0,09 % sodium azide, 50 % glycerol
Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species. Protein tyrosine nitration results in a post-translational modification, component of nitric oxide signaling.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
The antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivity with non-nitrated tyrosine. Not species specific.0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot.Antibody works paraffin-embedded sections.
Application Details:
1: 100 (IHC), 1: 1400 (WB), The exact and optimal working dilution should be determined by the investigator
Conjugation:
IgG2A
Isotype:
IgG2A
Purity:
Total IgG. Protein G purified, in PBS. Contains 50 % glycerol and 0.09% sodium azide.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.Antibody used in immunohistochemistry:Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387.
Special application note:
1 mg/ml of Protein G purified IgG2A in PBS pH 7,4, 0,09 % sodium azide, 50 % glycerol
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Bovine, avian
Expected Species:
Higher plants
Immunogen:
acetylated KLH
Applications:
ELISA (ELISA), Immunocytochmistry/Immunofluorescence (ICC/IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
1 g of this antibody is sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 g total protein and ECL detection system
Application Details:
1 : 100 (IHC), 1 : 1000 (WB)
Conjugation:
IgG1
Isotype:
IgG1
Purity:
Total IgG fraction. Protein G purified.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Vigushin & Coombes (2004). Targeted histone deacetylase inhibition for cancer therapy. Curr. Cancer Drug Targets 4: 205-218.
Special application note:
Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/mlantibody detects Proteins containing acetylated lysine residues in ELISA and WBs, Does not detect non-acetylated lysine residues
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Bovine, avian
Expected Species:
Higher plants
Immunogen:
acetylated KLH
Applications:
ELISA (ELISA), Immunocytochmistry/Immunofluorescence (ICC/IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
1 g of this antibody is sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 g total protein and ECL detection system
Application Details:
1 : 100 (IHC), 1 : 1000 (WB)
Conjugation:
IgG1
Isotype:
IgG1
Purity:
Total IgG fraction. Protein G purified.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Vigushin & Coombes (2004). Targeted histone deacetylase inhibition for cancer therapy. Curr. Cancer Drug Targets 4: 205-218.
Special application note:
Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/mlantibody detects Proteins containing acetylated lysine residues in ELISA and WBs, Does not detect non-acetylated lysine residues
Oxidative derivate of guanosine is called 8-Hydroxyguanosine (8OHdG) and is used as a popular biomarker of oxidative stress.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Recognizes markers of oxidative damage to DNA (8-hydroxy-2’-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine)
Immunogen:
8-hydroxy-guanosine-BSA and – casein conjugates
Applications:
ELISA (ELISA), Immunoaffinity chromatography (IAP), Immunohistochemistry on frozen tissue and paraffin-embedded (IHC-Fr-P)
Protocol for immunostaining using this antibody can be found here.
Application Details:
The optimal working dilution should be determined by the investigator
Conjugation:
IgG2A
Isotype:
IgG2A
Purity:
Total IgG fraction. Protein G purified.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Poborilova et al. (2015). DNA hypomethylation concomitant with the overproduction of ROS induced by naphthoquinone juglone on tobacco BY-2 suspension cells. Environmental and Experimental Botany, Volume 113, May 2015, Pages 28–39.Haigh and Drew (2015). Cavitation during the protein misfolding cyclic amplification (PMCA) method - The trigger for de novo prion generation? Biochem Biophys Res Commun. 2015 Apr 17. pii: S0006-291X(15)00726-3. doi: 10.1016/j.bbrc.2015.04.048.
Special application note:
Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 0,65 mg/ml
Oxidative derivate of guanosine is called 8-Hydroxyguanosine (8OHdG) and is used as a popular biomarker of oxidative stress.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Recognizes markers of oxidative damage to DNA (8-hydroxy-2’-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine)
Immunogen:
8-hydroxy-guanosine-BSA and – casein conjugates
Applications:
ELISA (ELISA), Immunoaffinity chromatography (IAP), Immunohistochemistry on frozen tissue and paraffin-embedded (IHC-Fr-P)
Protocol for immunostaining using this antibody can be found here.
Application Details:
The optimal working dilution should be determined by the investigator
Conjugation:
IgG2A
Isotype:
IgG2A
Purity:
Total IgG fraction. Protein G purified.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Poborilova et al. (2015). DNA hypomethylation concomitant with the overproduction of ROS induced by naphthoquinone juglone on tobacco BY-2 suspension cells. Environmental and Experimental Botany, Volume 113, May 2015, Pages 28–39.Haigh and Drew (2015). Cavitation during the protein misfolding cyclic amplification (PMCA) method - The trigger for de novo prion generation? Biochem Biophys Res Commun. 2015 Apr 17. pii: S0006-291X(15)00726-3. doi: 10.1016/j.bbrc.2015.04.048.
Special application note:
Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 0,65 mg/ml
Tic40 is a component of the inner envelope membrane import complex (TIC) of plant chloroplasts. Tic40 has been proposed to function as a co-chaperone in the stromal chaperone complex that facilitates protein translocation across the inner membrane. Tic40 can be used as a cellular [compartment marker] for chloroplast inner envelope membrane.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Specific fluorescence in ICC has been observed for interphase nuclei as well as around centromer region (where Ser10 of histone H3 is phosphorylated) in mitotic chromosomes
Application Details:
1 : 100-1 : 500 (ICC), 2 l of antibody/500 l solution (ChIp-qPCR), 1: 500 (IF), 1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
15 | 17 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Farago et al. (2022) Small paraquat resistance proteins modulate paraquat and ABA responses and confer drought tolerance to overexpressing Arabidopsis plants. Plant Cell Environ. 2022 Jul;45(7):1985-2003. doi: 10.1111/pce.14338. Epub 2022 Apr 29. PMID: 35486392; PMCID: PMC9324991.Margaritopoulou et al (2021) Enriched HeK4me3 marks at Pm-0 resistance-related genes prime courgette against Podosphaera xanthii. Plant Physiol. 2021 Sep 21:kiab453. doi: 10.1093/plphys/kiab453. Epub ahead of print. Erratum in: Plant Physiol. 2021 Nov 11;: PMID: 34597395.Perlaza (2021). Organelle Size and Quality Control in Chlamydomonas Reinhardtii. UCSF. ProQuest ID: Perlaza_ucsf_0034D_12217. Merritt ID: ark:/13030/m5257z1d. Retrieved from https://escholarship.org/uc/item/1jg3874hSun et al. (2021) The epigenetic factor FVE orchestrates cytoplasmic SGS3-DRB4-DCL4 activities to promote transgene silencing in Arabidopsis. Sci Adv. 2021 Aug 4;7(32):eabf3898. doi: 10.1126/sciadv.abf3898. PMID: 34348894; PMCID: PMC8336953.Chen et al. (2019). Phalaenopsis LEAFY COTYLEDON1-Induced Somatic Embryonic Structures Are Morphologically Distinct From Protocorm-Like Bodies. Front Plant Sci. 2019 Nov 29;10:1594. doi: 10.3389/fpls.2019.01594. PMID: 31850050; PMCID: PMC6896055.
Special application note:
Cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii
Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Histone 3 (H3) located in nuclei, incorporated into chromatin, Present in nucleosome together with H2A, H2B and H4.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight 488.
Molecular Weight:
15 | 17 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
To be added when available. Antibody released in May 2023.
Special application note:
Cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii.DyLight 488 Amax = 493 nm, Emax = 519 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
Histone 3 (H3) located in nuclei, incorporated into chromatin, Present in nucleosome together with H2A, H2B and H4.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight 594.
Molecular Weight:
15 | 17 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
To be added when available. Antibody released in May 2023.
Special application note:
Cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii.DyLight 594 has Amax = 593 nm, Emax = 618 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
Histone 3 (H3) located in nuclei, incorporated into chromatin, Present in nucleosome together with H2A, H2B and H4.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight 650.
Molecular Weight:
15 | 17 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
To be added when available. Antibody released in May 2023.
Special application note:
Cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii.DyLight 650 has Amax = 652 nm, Emax = 672 nm. DyLight is a registered trademark of Thermofisher Inc., and its subsidiaries.
DEG15 is a peroxisomal Deg-protease with endopeptidase activity. This protease cleaves specifically substrates with Cys in the P1 and P2 position and acts as peroxisomal processing peptidase. Alternative names: At1g28320/F3H9_2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Micromonas sp., Oryza sativa, Populus balsamifera, Solanum lycopersicum, Sorghum vulgare, Ricinus communis, Vitis vinifera, Zea mays Species of your interest not listed? Contact us
Immunogen:
synthetic peptide derived from Arabidopsis thaliana DEG15, Q8VZD4 (At1g28320)
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Schuhmann et al. (2008). The DEG15 serine protease cleaves peroxisomal targeting signal 2-containing proteins in Arabidopsis.Plant Physiol. 4: 1847-1856.Helm et al. (2007). Dual specificities of the glyoxysomal/peroxisomal processing protease Deg15 in higher plants.PNAS 27: 11501-11506.
α-Amylases are hydrolytic enzymes responsible for the mobilization of the starch into metabolizable sugars. This process provides the energy for the growth of roots and shoots and is crucial during germination of cereal seeds.These enzymes are coded by a multigene family and even thought other amylolytic enzyme participate in the process of starch breakdown, the contribution of α-amylase is the prerequisite for the initiation of this process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Oryza sativa, Panicum virgatum
Expected Species:
Cereals, Hordeum vulgare, Kalanchoe laxifloraSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known Oryza sativa P17654
Ye et al. (2018). Natural variation in the promoter of rice calcineurin B-like protein10 (OsCBL10) affects flooding tolerance during seed germination among rice subspecies. Plant J. 2018 May;94(4):612-625. doi: 10.1111/tpj.13881.
Special application note:
Antibody will detect all alpha amylase isoforms from rice, barley and other cereals
FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules. Synonymes: sifB, SulB.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Vedyaykin et al. (2020). SulA is able to block cell division in Escherichia coli by a mechanism different from sequestration. Biochem Biophys Res Commun . DOI: 10.1016/j.bbrc.2020.03.012 Ranjit et al. (2020). Chlamydial MreB Directs Cell Division and Peptidoglycan Synthesis in Escherichia coli in the Absence of FtsZ Activity. mBio. 2020 Feb 18;11(1). pii: e03222-19. doi: 10.1128/mBio.03222-19. (Immunofluorescence)Sekar et al. (2018). Synthesis and degradation of FtsZ quantitatively predict the first cell division in starved bacteria. Mol Syst Biol. 2018 Nov 5;14(11):e8623. doi: 10.15252/msb.20188623.M ckl et al. (2018). Filamentation and restoration of normal growth in Escherichia coli using a combined CRISPRi sgRNA/antisense RNA approach. PLoS One. 2018 Sep 11;13(9):e0198058. doi: 10.1371/journal.pone.0198058. eCollection 2018.Pende et al. (2014). Size-independent symmetric division in extraordinarily long cells. Nat Commun. 2014 Sep 15;5:4803. doi: 10.1038/ncomms5803.S derstr m et al. (2014). Disassembly of the divisome in Escherichia coli: Evidence that FtsZ dissociates before compartmentalisation. Mol Microbiol. 2014 Feb 7. doi: 10.1111/mmi.12534. (western blot and immunofluorescence)
α-Amylases are hydrolytic enzymes responsible for the mobilization of the starch into metabolizable sugars. This process provides the energy for the growth of roots and shoots and is crucial during germination of cereal seeds.These enzymes are coded by a multigene family and even thought other amylolytic enzyme participate in the process of starch breakdown, the contribution of α-amylase is the prerequisite for the initiation of this process. Ramy3D is one of the alpha amylases genes in the rice multigene family (Huang et al. Nucleic Acid Research 1990).Synonymes:1,4-alpha-D-glucan glucanohydrolase
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Oryza sativa
Immunogen:
KLH-conjugated synthetic peptide derived from known Oryza sativa P27933
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Ye et al. (2018). Natural variation in the promoter of rice calcineurin B-like protein10 (OsCBL10) affects flooding tolerance during seed germination among rice subspecies. Plant J. 2018 May;94(4):612-625. doi: 10.1111/tpj.13881.Ho et al. (2017). A calcineurin B-like protein participates in low oxygen signalling in rice. CSIRO PUBLISHING Functional Plant Biology.
Variant histone H2A may replace conventional H2A in a subset of nucleosomes. Synonymes: H2A.F/Z 3
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Bieluszewski et al. (2022) NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis. Nat Commun. 2022 Jan 12;13(1):277. doi: 10.1038/s41467-021-27882-5. PMID: 35022409; PMCID: PMC8755797.Bieluszewski et al. (2022) NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis. Nat Commun. 2022 Jan 12;13(1):277. doi: 10.1038/s41467-021-27882-5. PMID: 35022409; PMCID: PMC8755797.Kralemann et al. (2020). Removal of H2Aub1 by ubiquitin-specific proteases 12 and 13 is required for stable Polycomb-mediated gene repression in Arabidopsis. Genome Biol. 2020 Jun 16;21(1):144.doi: 10.1186/s13059-020-02062-8. G mez-Zambrano et al. (2019). The repressive role of Arabidopsis H2A.Z in transcriptional regulation depends on AtBMI1 activity. Nat Commun. 2019 Jun 27;10(1):2828. doi: 10.1038/s41467-019-10773-1. G mez-Zambrano et al. (2018). Arabidopsis SWC4 Binds DNA and Recruits the SWR1 Complex to Modulate Histone H2A.Z Deposition at Key Regulatory Genes. Mol Plant. 2018 Mar 29. pii: S1674-2052(18)30122-9. doi: 10.1016/j.molp.2018.03.014.
PHOT1 | phototropin-1 is a blue-light photoreceptor which containst light activated serine-threonine kinase domain. Is required for stomatal opening, chloroplast movements, leaf flattening and phototropism. Undergoes blue-light-dependent autophosphorylation. Alternative names: NPH1, RPT1, JK224, Non-phototropic hypocotyl protein 1, Root phototropism protein 1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana PHOT1 O48963, At3g45780
Information about phot1 mutant, first named nph1: Liscum &. Briggs (1995). Mutations in the NPH1 Locus of Arabidopsis Disrupt the Perception of Phototropic Stimuli. The Plant Cell, Vol. 7, 473-485.Huala et al. (1997). Arabidopsis NPH1: A Protein Kinase with a Putative Redox-Sensing Domain. Science 19: Vol. 278 no. 5346 pp. 2120-2123.Lehmann et al. (2011). Transitions of gene expression induced by short-term blue light. Plant Biology Volume 13, Issue 2, pages 349–361. Seeds of this mutant are available at uNASC.
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
111 | 132 kDa
Not reactive in:
Cuscuta campestris, Oryza sativa
Selected references:
Labuz et al. (2021) Phototropin interactions with SUMO proteins. Plant Cell Physiol. 2021 Feb 17:pcab027. doi: 10.1093/pcp/pcab027. Epub ahead of print. PMID: 33594440.Krzeszowiec et al. (2020). Chloroplasts in C3 grasses move in response to blue-light. Plant Cell Rep . 2020 Oct;39(10):1331-1343.doi: 10.1007/s00299-020-02567-3. Epub 2020 Jul 13.Labuz et al. (2015). The impact of temperature on blue light induced chloroplast movements in Arabidopsis thaliana. Plant Science, doi:10.1016/j.plantsci.2015.07.013.Eckstein et al. (2015). Auxin and chloroplast movements. Physiol Plant. 2015 Oct 15. doi: 10.1111/ppl.12396.
Special application note:
This product can be sold containing ProClin if requested
PHOT2 | phototropin-2 is a membrane-bound serine/threonine kinase that functions as blue light photoreceptor in redundancy with PHOT1. Involved in processed like stomatal opening, chloroplast movement and phototropism. Alternative names: NPL1, K21L19.6, AtKin7, Defective in chloroplast avoidance protein 1Non-phototropic hypocotyl 1-like protein 1, NPH1-like protein 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana PHOT2 P93025, At5g58140
Labuz et al. (2021) Phototropin interactions with SUMO proteins. Plant Cell Physiol. 2021 Feb 17:pcab027. doi: 10.1093/pcp/pcab027. Epub ahead of print. PMID: 33594440.Krzeszowiec et al. (2020). Chloroplasts in C3 grasses move in response to blue-light. Plant Cell Rep . 2020 Oct;39(10):1331-1343.doi: 10.1007/s00299-020-02567-3. Epub 2020 Jul 13.Labuz et al. (2015). The impact of temperature on blue light induced chloroplast movements in Arabidopsis thaliana. Plant Science, doi:10.1016/j.plantsci.2015.07.013.Aggarwal et al. (2014). Blue-light-activated phototropin2 trafficking from the cytoplasm to Golgi/post-Golgi vesicles. J Exp Bot. 2014 May 12.
Special application note:
This product can be sold containing ProClin if requested
GDP-L-Galactose Phosphorylase is a histidine triad (HIT) enzyme of the Smirnoff-Wheeler pathway of ascorbic acid synthesis in plants. Encoded by VTC2 gene. The enzyme catalyzes the conversion of GDP-L-galactose to L-galactose 1-phosphate in a reaction that consumes inorganic phosphate and produces GDP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica oleracea var. italica, Brassica rapa var. komatsuna, Citrus limon, Nicotiana tabacum, Spinacia oleracea, Zea mays, Chlamydomonas reinhardtii
Expected Species:
Manihot esculenta, Sorghum bicolor, Oryza sativa, Physcomitrium patensSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjuated synthetic peptide derived from known GDP-L-Galactose Phosphorylase sequences, including Arabidopsis thaliana Q8LKQ7 (At4g26850) and Chlamydomonas reinhardtii
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
Based on IEP, no reactivity is observed to non-immunoglobulin chicken serum proteins
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Concentration: > 4.5 mg/ml (E 1% at 280 nm = 14.0)
No reactivity is observed to non-immunoglobulin proteins from guinea pig serum
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.
Goat anti-human IgA heavy (alpha chain) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgA alpha chains in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgA heavy (alpha chains)
Expected Species:
Human IgA Heavy (alpha chains)
Immunogen:
purified human IgA, alpha chain
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
No confirmed exceptions from predicted reactivity are currently known
Special application note:
AP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Goat anti-human IgA (α chain), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Human IgA (α chain) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Human IgADyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (α) chains on human IgA (α chain)No reactivity is observed to: non-immunoglobulin human serum proteins, light chains on all human immunoglobulins
Goat anti-human IgA heavy (alpha chain) is a secondary antibody conjugated to HRP which binds to human IgA heavy (alpha chain) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgA heavy (alpha chain)
Expected Species:
Human IgA Heavy (alpha chain)
Immunogen:
purified human IgA, heavy (alpha chain)
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to the light chains or non-immunoglobulin human serum proteins
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
No reactivity is observed to the light chains or non-immunoglobulin human serum proteins based on immunoelectrophoresis.No reactivity to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.
Goat anti-human IgA heavy (alpha chain) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgA heavy, alpha chains in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgA, heavy alpha chains
Expected Species:
Human IgA, Heavy alpha chains
Immunogen:
purified human IgA, alpha chain
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to light chains or non-IgA human serum proteins based in immunoelectrophoresisno reactivity to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis
No confirmed exceptions from predicted reactivity are currently known
Special application note:
AP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Goat anti-human IgA heavy (alpha chain) is a secondary antibody conjugated to biotin which binds to human IgA heavy alpha chains in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. Shelf life is 1 year from date of receipt.
No reactivity is observed to light chains or non-IgA human serum proteins based on immunoelectrophoresis.No reactivity to bovine, mouse, rabbit serum based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative
Goat anti-human IgA heavy (alpha chain) is a secondary antibody conjugated to HRP which binds to human IgA heavy (alpha chain) in immunological assays. It is especially adsorbed against bovine/mouse/rabbit serum and affinity purified.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgA heavy chain (alpha)
Expected Species:
Human IgA Heavy chain (alpha)
Immunogen:
purified human IgA, heavy (alpha chain)
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to the light chains or non-immunoglobulin human serum proteins.No reactivity to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Goat anti-human IgA heavy (alpha chain), F(ab)'2 is a secondary antibody which binds to human IgA heavy (alpha chain), F(ab)'2 fragment in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life is one year from date of receipt.
No reactivity is observed to the light chains or non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody reacts with the alpha chains on human IgA based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 3 mg/ml.Antibody purity ≥90% based on SDS-PAGE. May contain small amounts of intact IgG.
Goat anti-human IgA (alpha chain) is a secondary antibody conjugated to biotin which binds to human IgA heavy (alpha chains) F(ab)'2 fragments in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. Shelf life is 1 year from date of receipt.
No reactivity is observed to other immunoglobulins or non-immunoglobulin human serum proteins based on immunoelectrophoresis
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative,Purity is ‰ 90% based on SDS-PAGE, May contain small amounts of intact IgG
Goat anti-human IgA heavy (alpha chain) is a secondary antibody conjugated to HRP which binds to human IgA heavy (alpha chain) F(ab)'2 fragments in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgA heavy chain (alpha) F(ab)'2 fragment
Expected Species:
Human IgA Heavy chain (alpha) F(ab)'2 fragment
Immunogen:
purified human IgA, heavy (alpha chain)
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to the light chains or non-immunoglobulin human serum proteins
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 0,55 ml of sterile water
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidasePurity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.
Alcohol dehydrogenase (E.C.:1.1.1.1) is an important enzyme for plants and microbes. In microalgae and bacteria the conversion of Acetyl-CoA to ethanol under conditions of oxygen deprivation is catalyzed by the dual function enzyme alcohol/acetaldehyde dehydrogenase (ADH/ALDH; E.C.:1.1.1.1 /1.2.1.10). This reaction results in NAD+ recycling and allows glycolysis to proceed.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Kurylo et al. (2018). Endogenous rRNA Sequence Variation Can Regulate Stress Response Gene Expression and Phenotype. Cell Rep. 2018 Oct 2;25(1):236-248.e6. doi: 10.1016/j.celrep.2018.08.093.Laurenceau et al. (2015). Conserved Streptococcus pneumoniae Spirosomes Suggest a Single Type of Transformation Pilus in Competence. PLoS Pathog. 2015 Apr 15;11(4):e1004835. doi: 10.1371/journal.ppat.1004835.Kukuczka et al. (2014). Proton Gradient Regulation5-Like1-Mediated Cyclic Electron Flow Is Crucial for Acclimation to Anoxia and Complementary to Nonphotochemical Quenching in Stress Adaptation. Plant Physiol. 2014 Jun 19;165(4):1604-1617.
Special application note:
Selected peptide is well conserved in Escherichia coli ADHE (P0A9Q7), most of the microbial dual function aldehyde/alcohol dehydrogenases (ADHE) and Iron-containing alcohol dehydrogenases are also conserved in a peptide used to elicit ADH antibody
No reactivity is observed to the light chains or non-immunoglobulin human serum proteins based on immunoelectrophoresis
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.
Goat anti-human IgE (epsilon chain) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgE (epsilon chain) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity, Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol, Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human Human IgE (epsilon chain)
Expected Species:
Human Human IgE (epsilon chain)
Immunogen:
purified human IgE
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Goat anti-human IgE heavy (epsilon chain) is a secondary antibody conjugated to HRP which binds to human IgE heavy (epsilon chain) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgE heavy (epsilon chain)
Expected Species:
Human IgE Heavy (epsilon chain)
Immunogen:
purified human IgE
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to the light chains or non-immunoglobulin human serum proteins
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidaseThe amount of cross-reactivity to human IgG/M/A has been tested, and it is very low. During manufacturing of this product, cross-reactivity to other IgG is removed. Please see the percentage of measured cross-reactivity to other human immunoglobulins below: Human IgG: 0.12 % Human IgA: 0.09 % Human IgM: 0.17 %
No reactivity is observed to the light chains or non-immunoglobulin human serum proteins based on immunoelectrophoresis.No reactivity to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.
Goat anti-human IgE heavy (epsilon chain) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgE heavy (epsilon chain) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgE heavy (epsilon chain)
Expected Species:
Human IgE Heavy (epsilon chain)
Immunogen:
purified human IgE
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to light chains or non-IgE human serum proteins based in immunoelectrophoresisno reactivity to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Goat anti-human IgE heavy (epsilon chain) is a secondary antibody conjugated to biotin which binds to human IgE heavy (epsilon chain) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. Shelf life is 1 year from date of receipt.
No reactivity is observed to light chains or non-IgE human serum proteins based on immunoelectrophoresis.No reactivity to bovine, mouse, rabbit serum based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative
Goat anti-human IgE (ε chain), DyLight 488 Conjugated, min. cross-reactivity to bovine, mouse, rabbit serum is a secondary antibody conjugated to DyLight 488, which binds to Human IgE (ε chain) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Human IgEDyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on human IgE (ε chain)No reactivity is observed to: non-immunoglobulin human serum proteins, light chains on all human immunoglobulins, serum proteins from bovine, mouse or rabbit
Goat anti-human IgE heavy (epsilon chain) is a secondary antibody conjugated to HRP which binds to human IgE heavy (epsilon chain) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgE heavy (epsilon chain)
Expected Species:
Human IgE Heavy (epsilon chain)
Immunogen:
purified human IgE
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
No reactivity is observed to the light chains or non-immunoglobulin human serum proteins.No reactivity to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 7.49 mg/ml.
Goat anti-human IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG (H&L)
Expected Species:
Human IgG (H&L)
Immunogen:
Purified human IgG
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with alpha (heavy chains) on human IgG and all light chains on human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins based in immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative
Goat anti-human IgG (H&L) is a secondary antibody conjugated to HRP which binds to human IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Heavy chains on human IgG and light chanins on all human immunoglobulins
Immunogen:
Purified human IgG, whole molecule
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Not reactive in:
Non immunoglobulin human serum proteins
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 10 % (w/v) BSA, Protease/IgG free with 0,1 % (v/v) of ProClin 150 is used as preservative
Goat anti-human IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Human IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Human IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on human IgG (γ chain), light chains on all human immunoglobulinsNo reactivity is observed to: non-immunoglobulin human serum proteins
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum or bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.
Goat anti-human IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG (H&L)
Expected Species:
Human IgG (H&L)
Immunogen:
Purified human IgG
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy (gamma) chains on human IgG and all lights chains of human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins and serum proteins from mouse, rabbit or bovine based on immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins or serum from bovine, mouse, rabbit based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative
Goat anti-human IgG (H&L) is a secondary antibody conjugated to HRP which binds to human IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG (H&L)
Expected Species:
Human IgG (H&L)
Immunogen:
Purified human IgG
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins or bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 3 mg/ml.Antibody purity ≥90% based on SDS-PAGE. May contain small amounts of intact IgG.
Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG (H&L)
Expected Species:
Human IgG (H&L)
Immunogen:
Purified human IgG
Applications:
ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the alpha chains on human IgG based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.Purity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.
This antibdy reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to human IgG (H&L), F(ab)'2 fragment in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG (H&L), F(ab)'2 fragment
Expected Species:
Human IgG (H&L), F(ab)'2 fragment
Immunogen:
purified human IgG (H&L)
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 0,55 ml of sterile water
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidasePurity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum or bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 3 mg/ml.
Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L), F(ab)'2 fragment in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG (H&L), F(ab)'2 fragment
Expected Species:
Human IgG (H&L), F(ab)'2 fragment
Immunogen:
Purified human IgG (H&L)
Applications:
ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy (gamma) chains on human IgG and light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins and serum from mouse, rabbit of bovine serum proteins based on immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.Purity ≥90% based on SDS-PAGE. May contain small amounts of intact IgG.
Goat anti-human IgG (H&L), F(ab)'2 fragment, is a secondary antibody conjugated to biotin which binds to human IgG (H&L), F(ab)'2 fragment in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. Shelf life is 1 year from date of receipt.
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins or serum proteins from bovine, mouse, rabbit based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative,Purity ‰ 90 % based on SDS-PAGE, May contain small amounts of intact IgG
Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to human IgG (H&L), F(ab)'2 fragment in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG (H&L), F(ab)'2 fragment
Expected Species:
Human IgG (H&L), F(ab)'2 fragment
Immunogen:
purified human IgG (H&L)
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins or bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidasePurity ≥90 % based on SDS-PAGE. May contain small amounts of intact IgG
Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life is one year from date of receipt.
The optimal working dilution should be determined by the investigator, Antibody is sutitable for all immunoassay applications
Purity:
Immunogen affinity purified goat IgG.
Special application note:
Concentration: > 4.5 mg/ml (E 1% at 280 nm = 13.0)Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of sodium azide as preservative.Antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed to light chains on human immunoglobulins and non-immunoglobulin human serum proteins or to human IgG F(ab)'2 fragment based on immunoelectrophoresis.
Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG Fc (two heavy chains with constant domains)
Expected Species:
Human IgG Fc (two Heavy chains with constant domains)
Immunogen:
Purified human IgG
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunoglobulins and non-immunoglobulin human serum proteins and human IgG F(ab)'2 fragment based in immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to biotin which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. Shelf life is 1 year from date of receipt.
Host Animal:
Goat
Species Reactivity:
Human IgG Fc (two heavy chains with constant domains)
Expected Species:
Human IgG Fc (two Heavy chains with constant domains)
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed to immunoglobulin light chains, non-immunoglobulin human serum proteins or IgG F(ab)'2 based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative
Goat anti-human IgG Fc, DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Human IgG Fc in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Human IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on human IgGNo reactivity is observed to: non-immunoglobulin human serum proteins, light chains on all human immunoglobulins
Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to HRP which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG Fc (two heavy chains with constant domains)
Expected Species:
Human IgG Fc (two Heavy chains with constant domains)
Immunogen:
Purified human IgG
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed to the light chains on human immunoglobulins or non-immunoglobulin human serum proteins or human IgG F(ab)'2 fragment based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life is one year from date of receipt.
Host Animal:
Goat
Species Reactivity:
Human IgG Fc (two heavy chains with constant domains)
Expected Species:
Human IgG Fc (two Heavy chains with constant domains)
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunoglobulins ornon-immunoglobulin human serum or bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.This antibody has been absorbed against solid phase normal bovine, mouse and rabbit serum in addition to mouse and rabbit IgG.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.
Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG Fc (two heavy chains with constant domains)
Expected Species:
Human IgG Fc (two Heavy chains with constant domains)
Immunogen:
Purified human IgG
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy (gamma) chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunoglobulins, human IgG F(ab)' fragment or non-immunoglobulin human serum or bovine, mouse or rabbit serum proteins based on immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to biotin which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. Shelf life is 1 year from date of receipt.
Host Animal:
Goat
Species Reactivity:
Human IgG Fc (two heavy chains with constant domains)
Expected Species:
Human IgG Fc (two Heavy chains with constant domains)
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed to the light chains on human immunoglobulins, non-immunoglobulin human serum proteins or serum from bovine, mouse, rabbit based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative
Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to HRP which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG Fc (two heavy chains with constant domains)
Expected Species:
Human IgG Fc (two Heavy chains with constant domains)
Immunogen:
Purified human IgG
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunglobulins or non-immunoglobulin human serum proteins or bovine, mouse or rabbit serum proteins or IgG F(ab)' fragment based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Goat anti-human IgG Fc (two heavy chains with constant domains)is a secondary antibody which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG Fc (two heavy chains with constant domains)
Expected Species:
Human IgG Fc (two Heavy chains with constant domains),Human IgG (H&L)
Antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed to light chains on human immunoglobulins and to human IgA and IgM based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.
Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG Fc (two heavy chains with constant domains)
Expected Species:
Human IgG Fc (two Heavy chains with constant domains)
Immunogen:
Purified human IgG
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunoglobulins and to human IgA or IgM based on immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to biotin which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. Shelf life is 1 year from date of receipt.
Host Animal:
Goat
Species Reactivity:
Human IgG Fc (two heavy chains with constant domains)
Expected Species:
Human IgG Fc (two Heavy chains with constant domains)
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed to immunoglobulin light chains, and human IgA and IgM based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative
Goat anti-human IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to HRP which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG Fc (two heavy chains with constant domains)
Expected Species:
Human IgG Fc (two Heavy chains with constant domains)
Immunogen:
Purified human IgG
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed to the light chains on human immunoglobulins or human IgA and IgM based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunoglobulins and non-immunoglobulin human serum proteins and human IgG F(ab)'2 fragment based in immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative,Purity is ‰ 90% based on SDS-PAGE, May contain small amounts of intact IgG
Antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 0,05 % (w/v) of Sodium azide as preservative
Goat anti-human IgG Fc (two heavy chains with constant domains) F(ab)'2 fragment is a secondary antibody which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life is one year from date of receipt.
Host Animal:
Goat
Species Reactivity:
Human IgG Fc (two heavy chains with constant domains)
Expected Species:
Human IgG Fc (two Heavy chains with constant domains)
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunoglobulins or non-immunoglobulin human serum or bovine, mouse or rabbit serum proteins or human F(ab)'2 fragment based on immunoelectrophoresis.This antibody has been absorbed against solid phase normal bovine, mouse and rabbit serum in addition to mouse and rabbit IgG.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 3 mg/ml.
Goat anti-human IgG Fc (two heavy chains with constant domains) F(ab)'2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG Fc (two heavy chains with constant domains)
Expected Species:
Human IgG Fc (two Heavy chains with constant domains)
Immunogen:
Purified human IgG
Applications:
ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy (gamma) chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunoglobulins, or non-immunoglobulin human serum or bovine, mouse or rabbit serum proteins or mouse and rabbit IgG based on immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Goat anti-human IgG Fc (two heavy chains with constant domains) F(ab)'2 fragment is a secondary antibody conjugated to biotin which binds to human IgG Fc (two heavy chains with constant domains) F(ab)'2 fragment in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. Shelf life is 1 year from date of receipt.
Host Animal:
Goat
Species Reactivity:
Human IgG Fc (two heavy chains with constant domains) F(ab)'2 fragment
Expected Species:
Human IgG Fc (two Heavy chains with constant domains) F(ab)'2 fragment
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed to the light chains on human immunoglobulins, non-immunoglobulin human serum proteins or serum from bovine, mouse, rabbit based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative
Goat anti-human IgG Fc (two heavy chains with constant domains) F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to human IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Human IgG Fc (two heavy chains with constant domains)
Expected Species:
Human IgG Fc (two Heavy chains with constant domains)
Immunogen:
Purified human IgG
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.No reactivity is observed with the light chains on human immunglobulins or non-immunoglobulin human serum proteins or bovine, mouse or rabbit serum proteins or IgG F(ab)' fragment based on immunoelectrophoresis.The antibody ahs been absorbed against solid phase normal bovine, mouse and rabbit serum in addition to mouse and rabbit IgG.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 0,55 ml of sterile water
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified chicken IgY.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 0,05 % (w/v) of Sodium azide as preservative
Goat anti-human IgM (μ or mu chain)is a secondary antibody which binds to human IgM (μ or mu chain) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Antibody reacts with the human IgM (μ or mu chain) based on immunoelectrophoresis.No reactivity is observed with IgG F(ab)'2 fragment or non-immunoglobulin human serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.
Antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 0,05 % (w/v) of Sodium azide as preservative
Goat anti-human IgM (μ chain) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgM (μ chain) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life is one year from date of receipt.
This antibody reacts with human IgM (μ chain) based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins or human IgG or IgA immunoglobulins based in immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.
Goat anti-human IgM (μ chain), no reactivity to human IgA+IgG is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgM (μ chain) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilution prior to use and then discard. Shelf life of this product is one year from date of receipt.
Host Animal:
Goat
Species Reactivity:
human IgM (? chain)
Immunogen:
Purified human IgM
Applications:
ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the human IgM (μ chain) based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins and to human IgA or IgG based on immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified chicken IgY.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.
Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Chicken
Species Reactivity:
Rabbit IgG (H&L)
Expected Species:
Rabbit IgG (H&L)
Immunogen:
Purified Rabbit IgG
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with alpha (heavy chains) on rabbit IgG and all light chains on rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
Chicken anti-Rabbit IgG (H&L) - DyLight 488 Conjugate is a secondary antibody conjugated to DyLight 488, which binds to Rabbit IgG (H&L) in immunological assays.DyLight 488 Amax = 493 nm, Emax = 519 nm. Antibodies are purified using solid phase Rabbit IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Kovaleva et al. (2017). Regulation of Petunia Pollen Tube Growth by Phytohormones: Identification of Their Potential Targets. DOI:10.17265/2161-6256/2016.04.004. (immunolocalization)
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG, light chains on all rabbit immunoglobulinsNo reactivity is observed to: non-immunoglobulin rabbit serum proteins
Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store non-diluted antibody at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Chicken
Species Reactivity:
Rabbit IgG (H&L)
Expected Species:
Rabbit IgG (H&L)
Immunogen:
Purified Rabbit IgG
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified chicken IgY.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Levitan et al. (2019). Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17316-17322. doi: 10.1073/pnas.1906726116.Gao et al. (2018). Cisgenic overexpression of cytosolic glutamine synthetase improves nitrogen utilization efficiency in barley and prevents grain protein decline under elevated CO2. Plant Biotech. J. 10.1111/pbi.13046
Special application note:
Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.2)HRP-conjugate is supplied in PBS, 1% BSA and 0.1% proclin 150.0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Chicken anti-rabbit IgG (H&L)is a secondary antibody which binds to rabbit IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum and human or mouse serum or IgG based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified chicken IgY.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.
Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Chicken
Species Reactivity:
Rabbit IgG (H&L)
Expected Species:
Rabbit IgG (H&L)
Immunogen:
Purified Rabbit IgG
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on rabbit IgG and the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins or to human or mouse serum or IgG based on immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
This antibody reacts with the heavy chains on rabbit IgG and the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum or human and mouse serum or IgG based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified chicken IgY.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative
Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Chicken
Species Reactivity:
Rabbit IgG (H&L)
Expected Species:
Rabbit IgG (H&L)
Immunogen:
Purified Rabbit IgG
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins and human and mouse serum and IgG on immunoelectrophoresis.This antibody was absorbed against solid phase human and mouse serum and IgG.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified chicken IgY.
Reconstitution:
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified donkey IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Antibody concentration is 4.5 mg/ml.
Donkey anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Donkey
Species Reactivity:
Rabbit IgG (H&L)
Expected Species:
Rabbit IgG (H&L)
Immunogen:
Purified Rabbit IgG
Applications:
ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with alpha (heavy chains) on rabbit IgG and all light chains on rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Kay et al. (2014). Elevations in Th2-initiating cytokines (IL-33, IL-25, TSLP) in lesional skin from Chronic Spontaneous ("Idiopathic") Urticaria. Br J Dermatol. 2014 Dec 18. doi: 10.1111/bjd.13621.
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified donkey IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative
Donkey anti-Rabbit IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Rabbit IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rabbit IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG, light chains on all rabbit immunoglobulinsNo reactivity is observed to: non-immunoglobulin rabbit serum proteins
Donkey anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store non-diluted antibody at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Donkey
Species Reactivity:
Rabbit IgG (H&L)
Expected Species:
Rabbit IgG (H&L)
Immunogen:
Purified Rabbit IgG
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified donkey IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Goat anti-rabbit IgG (H&L), F(ab)'2 fragment is a secondary antibody which binds to rabbit IgG in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.05 % (w/v) of Sodium azide as preservative.Purity is ≥ 90 % based on SDS-PAGE. May contain small amounts of intact IgG.
Sml1 is a ribonucleotide reductase inhibitor involved in regulation of dNTP production. It is regulated by Mec1p and Rad53p during DNA damage and S phase. Synonymes: Yml058w.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Saccharomyces cerevisiae
Expected Species:
Saccharomyces cerevisiae
Immunogen:
KLH-conjugated synthetic peptide derived from known S.cerevisie Sml1 sequence. Gene ID: 854945
Cell preparation for western blot: cells were harvested by centrifugation (4000 x g , 6 min, 4 C), Supernatant was discarded and cells were resuspended in 500 μl cold TCA buffer (20 mM Tris, pH 8, 50 mM ammonium acetate, 2 mM EDTA, 1 tablet/10 ml of Complete Mini Protease inhibitor cocktail with EDTA (Roche Diagnostics GmbH)), 500 μl 0,5 mm Zirconia/Silica Beads (BioSpec Products, Inc, 11079105z) and 500 μl cold 20 % TCA was added, Samples were vigorously vortexed twice for 30 sec (kept on ice in between), 750 μl from the liquid phase was transferred into a fresh Eppendorf tube, Samples were centrifuged for 10 min (20000 x g, 4 C), The pellet was resuspended in 300 μl TCA-Laemmli buffer and boiled for 10 min at 100 C
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
11,83 | 11-12 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Cerritelli et al. (2020). High density of unrepaired genomic ribonucleotides leads to Topoisomerase 1-mediated severe growth defects in absence of ribonucleotide reductase. Nucleic Acids Res Corcoles-Saez et al. (2019). Essential Function of Mec1, the Budding Yeast ATM/ATR Checkpoint-Response Kinase, in Protein Homeostasis. Dev Cell. 2018 Aug 20;46(4):495-503.e2. doi: 10.1016/j.devcel.2018.07.011.Garbacz et al. (2019). The absence of the catalytic domains of Saccharomyces cerevisiae DNA polymerase ϵ strongly reduces DNA replication fidelity. Nucleic Acids Res. 2019 Jan 30. doi: 10.1093/nar/gkz048.Golla et al. (2017). A systematic assessment of chemical, genetic, and epigenetic factors influencing the activity of anticancer drug KP1019 (FFC14A). Oncotarget. 2017 Sep 30;8(58):98426-98454. doi: 10.18632/oncotarget.21416.Dmowski et al. (2017). Mutations in the Non-Catalytic Subunit Dpb2 of DNA Polymerase Epsilon Affect the Nrm1 Branch of the DNA Replication Checkpoint. PLoS Genet. 2017 Jan 20;13(1):e1006572. doi: 10.1371/journal.pgen.1006572.Mertz et al. (2015). Colon cancer-associated mutator DNA polymerase δ variant causes expansion of dNTP pools increasing its own infidelity. Proc Natl Acad Sci U S A. 2015 May 12;112(19):E2467-76. doi: 10.1073/pnas.1422934112. Epub 2015 Mar 31.Singh et al. (2014). Anti-cancer drug KP1019 modulates epigenetics and induces DNA damage response in Saccharomyces cerevisiae. FEBS Lett. 2014 Feb 20. pii: S0014-5793(14)00137-9. doi: 10.1016/j.febslet.2014.02.017.Azad et al. (2013). Depletion of Cellular Iron by Curcumin Leads to Alteration in Histone Acetylation and Degradation of Sml1p in Saccharomyces cerevisiae. PLoS One, March 8.Poli et al. (2012).dNTP pools determine fork progression and origin usage under replication stress. The EMBO J. January 2012, 1-12.
Goat anti-rabbit IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to rabbit IgG (H&L) immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Rabbit IgG (H&L)
Expected Species:
Rabbit IgG (H&L)
Immunogen:
Purified Rabbit IgG
Applications:
ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known
Special application note:
ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.Purity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.
This antibdy reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative,Purity is ‰ 90% based on SDS-PAGE, May contain small amounts of intact IgG
Goat anti-rabbit IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Rabbit IgG (H&L)
Expected Species:
Rabbit IgG (H&L)
Immunogen:
Purified Rabbit IgG (H&L)
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Linster et al. (2015). Downregulation of N-terminal acetylation triggers ABA-mediated drought responses in Arabidopsis. Nat Commun. 2015 Jul 17;6:7640. doi: 10.1038/ncomms8640.
Special application note:
Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidasePurity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.
Goat anti-rabbit IgG Fc (two heavy chains with constant domains)is a secondary antibody which binds to rabbit IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life is one year from date of receipt.
This antibody reacts with the heavy chains on rabbit IgG based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins and with the light chains of rabbit IgG based in immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Antibody is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.005 % sodium azide is added as preservative.Antibody concentration is 4.5 mg/ml.
Goat anti-rabbit IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to biotin which binds to rabbit IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. Shelf life is 1 year from date of receipt.
Host Animal:
Goat
Species Reactivity:
rabbit IgG Fc (two heavy chains with constant domains)
Expected Species:
Rabbit IgG Fc (two Heavy chains with constant domains)
This antibody reacts with the heavy chains on rabbit IgG based on immunoelectrophoresis.No reactivity is observed to immunoglobulin light chains or non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Biotin conjugate is supplied in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, Contains 0,05 % (w/v) sodium azide as preservative
Goat anti-rabbit IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to HRP which binds to rabbit IgG Fc (two heavy chains with constant domains) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
rabbit IgG Fc (two heavy chains with constant domains)
Expected Species:
Rabbit IgG Fc (two Heavy chains with constant domains)
Immunogen:
Purified Rabbit IgG
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on rabbit IgG based on immunoelectrophoresis.No reactivity is observed to the light chains on rabbit immunoglobulins or non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.This antibody will cross react with the heavy chain on other species IgG, but not with IgM or IgA.
Application Details:
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase
Bovine IgG fraction contains total bovine IgG from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Bovine immunoglobulin fraction is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. and 0.005 % sodium azide is added as preservative.Concentration is 4.5 mg/ml.
Purified bovine IgG contains Protein A purified bovine IgG from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Bovine IgG is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.005 % sodium azide is added as preservative.Concentration is 4.5 mg/ml.
Chicken IgY immunoglobulin fraction contains chicken immunoglobulin Y from non immunized animals and is excellent for use as blocking reagent in immunoassays like ELISA and Western blot.
Total IgY purified by PEG precipitation in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2.
Special application note:
Chicken IgY is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.05 % sodium azide is added as preservative.Concentration is >4.5 mg/ml (E 1% at 280 nm = 13.2). Purity > 80% based on SDS-PAGE.
Horse Ig fraction contains total horse immunoglobulins from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Purified horse IgG contains Protein A purified horse IgG from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Total IgG. Protein A purified, in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Contains 0.05 % sodium azide.
Special application note:
Horse IgG is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.005 % sodium azide is added as preservative.Concentration: > 4.5 mg/ml (E 1% at 280 nm = 13.0)
Guinea pig Ig fraction contains total guinea pig immunoglobulins from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Ig fraction obtained by a proprietary 2-step procedure, in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Contains 0.05 % sodium azide.
Special application note:
Guinea pig immunoglobulin fraction is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.005 % sodium azide is added as preservative.Concentration is 4.5 mg/ml.
Purified guinea pig IgG contains Protein A purified guinea pig IgG from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Goat IgG fraction contains total goat Ig from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Ig fraction obtained by a proprietary 2-step procedure, in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Contains 0.05 % sodium azide.
Special application note:
Goat immunoglobulin fraction is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.005 % sodium azide is added as preservative.Concentration is 4.5 mg/ml.
Purified goat IgG contains Protein G purified goatIgG from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Hamster IgG fraction contains total hamster Ig from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Total IgG. Protein A purified in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Contains 0.05 % sodium azide.
Special application note:
Human IgG is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.005 % sodium azide is added as preservative.Concentration is 4.5 mg/ml.
Purified IgG, Fc fragment in 50mM TRIS, pH 8.0, 0.2 M NaCl and 0.05 % sodium azide.
Special application note:
Concentration: > 1.0 mg/ml (E 1% at 280 nm = 13.0)Human IgG is provided in 50 mM Tris, 200 mM sodium chloride, pH 8 0.05 % sodium azide is added as preservative.Purity >95 % by SDS-PAGE. Expected MW of human Fc fragment is 26 kDa. On SDS-PAGE performed in reduced conditions it migrates below 31 kDa.Donor serum was tested by approved methods and found negative for antibodies to HIV 1/2, HCV, HBsAg, HIV-1 RNA, HCV and HBc.
Purified human IgG, F(ab)'2 fragment contains Protein A purified human IgG from normal serum and is excellent for use as blocking reagent in immunoassays.
Purified IgG, F(ab)'2 fragment in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2.
Special application note:
Human IgG, F(ab)'2 fragment is provided in 10 mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 0.05 % sodium azide is added as preservative.Purity >95 % by SDS-PAGE.Donor serum was tested by approved methods and found negative for antibodies to HIV 1/2, HCV, HBsAg, HIV-1 RNA, HCV and HBc.
Mouse Ig fraction contains total mouse Ig from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Ig fraction obtained by a proprietary 2-step procedure, in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Contains 0.05 % sodium azide.
Special application note:
Mouse immunoglobulin fraction is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.005 % sodium azide is added as preservative.Concentration is 4.5 mg/ml.
Purified mouse IgG contains Protein A purified mouse IgG from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Total IgG. Protein A purified in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Contains 0.05 % sodium azide.
Special application note:
Mouse IgG is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.05 % sodium azide is added as preservative.This immunoglobulin preparation can be used as a negative control for immunoprecipitation (IP).Antibody will react with all IgG subclasses.
Rabbit IgG fraction contains total rabbit Ig from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Ig fraction obtained by a proprietary 2-step procedure, in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Contains 0.05 % sodium azide.
Special application note:
Rabbit immunoglobulin fraction is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.005 % sodium azide is added as preservative.Concentration is 4.5 mg/ml.Rabbit serum can be used as a blocking reagent at 2-5% for primary antibodies developed in a rabbit.
Purified rabbit IgG contains Protein A purified rabbit IgG from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
The optimal dilution has to be determined by end user.
Purity:
Total IgG. Protein A purified IgG in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Contains 0.05 % sodium azide.
Special application note:
Rabbit IgG is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.05 % sodium azide is added as preservative. Concentration is 11.4 mg/ml. Purity > 95 % based on SDS-PAGEAntibody is suitable as a blocking reagent or a control.
Purified rabbit IgG contains Protein A purified rabbit IgG from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
The optimal dilution has to be determined by end user.
Purity:
Total IgG. Protein A purified IgG in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Contains 0.05 % sodium azide.
Special application note:
Rabbit IgG is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.05 % sodium azide is added as preservative.Purity > 95 % based on SDS-PAGE
Purified rabbit IgG contains Protein A purified rabbit IgG from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
The optimal dilution has to be determined by end user.
Purity:
Total IgG. Protein A purified IgG in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Contains 0.05 % sodium azide.
Special application note:
Rabbit IgG is provided in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. 0.05 % sodium azide is added as preservative.Purity > 95 % based on SDS-PAGE
AKIN11 (E.C.= 2.7.11.1) is a catalytic subunit of the putative trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. Synonymes: AKIN alpha-1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Gutierrez-Beltran et al. (2021) Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis. EMBO J. 2021 Jul 21:e105043. doi: 10.15252/embj.2020105043. Epub ahead of print. PMID: 34287990.Pedrotti et al. (2018). Snf1-RELATED KINASE1-Controlled C/S1-bZIP Signaling Activates Alternative Mitochondrial Metabolic Pathways to Ensure Plant Survival in Extended Darkness. Plant Cell. 2018 Feb;30(2):495-509. doi: 10.1105/tpc.17.00414. Epub 2018 Jan 18.Emanuelle et al. (2015). SnRK1 from Arabidopsis thaliana is an atypical AMPK. Plant J. 2015 Mar 3. doi: 10.1111/tpj.12813.
Purified rabbit IgG, F(ab)'2 fragment contains Protein A purified rabbit IgG from normal serum and is excellent for use as blocking reagent in immunoassays.
Purified IgG, F(ab)'2 fragment in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Contains 0.05 % sodium azide.
Special application note:
Rabbit IgG, F(ab)'2 fragment is provided in 10 mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 0.05 % sodium azide is added as preservative.Concentration is 4.5 mg/ml.Purity >95 % by SDS-PAGE.
Sheep Ig fraction contains total sheep Ig from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Ig fraction obtained by a proprietary 2-step procedure, in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Contains 0.05 % sodium azide.
Special application note:
Sheep immunoglobulin fraction is provided in 10 mM sodium phosphate, 150 M sodium chloride, pH 7.2. 0.05 % sodium azide is added as preservative.Concentration is 4.5 mg/ml.
Purified sheep IgG contains Protein G purified sheep IgG from normal serum, e.g. serum of non immunized animals and is excellent for use as blocking reagent in immunoassays.
Soluble oligomeric assemblies of the Amyloid-β peptide are today anticipated to be the direct cause regarding the Alzheimer pathology. As a consequence, oligomeric Aβ-assemblies constitute a very interesting therapeutic target. Identification of Aβ-oligomers is however, technically challenging due to there labile nature and low abundance. Abeta oligomer-specific OMAB antibody is based on the IgM isotype and represents a new concept of Aβ-oligomer binders using a combination of high avidity and very low monovalent affinity. This combination creates a selectivity of the antibody towards the oligomeric fraction and minimizes reactivity towards monomeric species.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human Abeta oligomers only
Expected Species:
Rat
Immunogen:
partly aggregated, recombinant peptide corresponding to the human Abeta (1-40/42), Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V, The epitope is 3-8, Molecular weight of immunogen is 4,5 kDa,
OMAB antibody is a versatile tool within research of Alzheimer’s disease, A sandwhich ELISA illustrates its potential regarding its high selectivity towards A? oligomers
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Pang et al (2021) An App knock-in rat model for Alzheimer's disease exhibiting A? and tau pathologies, neuronal death and cognitive impairments. Cell Res. 2021 Nov 17. doi: 10.1038/s41422-021-00582-x. Epub ahead of print. PMID: 34789895.Oh et al. (2020). Associative Interactions among Zinc, Apolipoprotein E, and Amyloid-? in the Amyloid Pathology. Int J Mol Sci. 2020 Jan 25;21(3). pii: E802. doi: 10.3390/ijms21030802.Henning-Knechtel et al. (2020). Designed Cell-Penetrating Peptide Inhibitors of Amyloid-beta Aggregation and Cytotoxicity. Cell Reports Physical Science,Volume 1, Issue 2, 26Zhang et al. (2019). Brains of rhesus monkeys display A? deposits and glial pathology while lacking A? dimers and other Alzheimer's pathologies. Aging Cell. 2019 Jun 4:e12978. doi: 10.1111/acel.12978.Kumar et al. (2018). Peptidomimetic-Based Multidomain Targeting Offers Critical Evaluation of A? Structure and Toxic Function. J Am Chem Soc. 2018 May 30;140(21):6562-6574. doi: 10.1021/jacs.7b13401.
Special application note:
OMAB antibody has been purified by by ion-exchange chromatography and is supplied in PBS without any additives as carrier proteins or sodium azide.Binding of OMAB antibody and Abeta oligomers at RT takes about 15 min.Fibrils are inaccessible for OMAB antibodies therefore if a discrimination between fibrils and oligomers is to be achieved, dot blot can be used. Start with antigen concentration of 500 ng/dot followed by 2X dilution steps. Blocking: non-fat milk and washes with 0.3 % Tween 20 in TBS pH 7.4.
Soluble oligomeric assemblies of the Amyloid-β peptide are today anticipated to be the direct cause regarding the Alzheimer pathology. As a consequence, oligomeric Aβ-assemblies constitute a very interesting therapeutic target. Identification of Aβ-oligomers is however, technically challenging due to there labile nature and low abundance. Abeta oligomer-specific OMAB antibody is based on the IgM isotype and represents a new concept of Aβ-oligomer binders using a combination of high avidity and very low monovalent affinity. This combination creates a selectivity of the antibody towards the oligomeric fraction and minimizes reactivity towards monomeric species.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Human Abeta oligomers only
Expected Species:
Rat
Immunogen:
partly aggregated, recombinant peptide corresponding to the human Abeta (1-40), Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V
OMAB antibody is a versatile tool within research of Alzheimer’s disease, A sandwhich ELISA illustrates its potential regarding its high selectivity towards A? oligomers
Application Details:
Coating antibody at 2 g/ml (ELISA), 1 : 500 (IHC)
Conjugation:
IgM
Isotype:
IgM
Purity:
Affinity purified in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 l of sterile water
Molecular Weight:
4,5 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Richman et al. (2013). In Vitro and Mechanistic Studies of an Anti-Amyloidogenic Self-Assembled Cyclic D,L-#-Peptide Architecture. J. Americal Chemical Societ, Jan 19.Lindhagen-Persson et al. (2010). Amyloid-β Oligomer Specificity Mediated by the IgM Isotype – Implications for a Specific Protective Mechanism Exerted by Endogenous Auto-Antibodies. PLoS ONE.
Special application note:
OMAB antibody has been purified by by ion-exchange chromatography and is supplied in PBS without any additives as carrier proteins or sodium azide. Binding of OMAB antibody and Abeta oligomers at RT takes about 15 min.Fibrils are inaccessible for OMAB antibodies therefore if a discrimination between fibrils and oligomers is to be achieved, dot blot can be used. Start with antigen concentration of 500 ng/dot followed by 2X dilution steps. Blocking: non-fat milk and washes with 0.3 % Tween 20 in TBS pH 7.4.
EF1A (elongation factor 1-alpha) belongs to the GTP-binding elongation factor family and is localized in the cytoplasm. It is an essential enzyme in elongation phase of protein synthesis. There are four genes encoding EF1A in Arabidopsis, sequences of genes 1,2 and 3 are identical. Synonymes:eEF-1A.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
This product can be sold containing ProClin if requested.Following protein resolubilization with urea/thiourea/CHAPS signal strength of recognized band is decreased. Recommended protein extraction conditions are without urea.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
49,5 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Pan et al. (2021) Post-Golgi Trafficking of Rice Storage Proteins Requires the Small GTPase Rab7 Activation Complex MON1-CCZ1, Plant Physiology, 2021;, kiab175, https://doi.org/10.1093/plphys/kiab175Ren et al. (2020). GPA5 Encodes a Rab5a Effector Required for Post-Golgi Trafficking of Rice Storage Proteins. Plant Cell. 2020 Jan 16. pii: tpc.00863.2019. doi: 10.1105/tpc.19.00863.Djuki? et al. (2019). Expression of protein synthesis elongation factors in winter wheat and oat in response to heat stress. Journal of Plant Physiology Volume 240, September 2019, 153015.Foley et al. (2017). A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate.Dev Cell. 2017 Apr 24;41(2):204-220.e5. doi: 10.1016/j.devcel.2017.03.018.
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KLH-conjugated synthetic peptide derived from known sequences of DSP in diatoms, conserved in Dsp1 and Dsp2, UniProt: B6RD07 of Thalassiosira pseudonana and Skeletonema costatum and Dsp1 in Fragilariopsis cylindrus and Phaeodactylum tricornutum
Thamatrakoln et al. (2013). Death-specific protein in a marine diatom regulates photosynthetic responses to iron and light availability. PNAS 2013 Dec 10;110(50):20123-8. doi: 10.1073/pnas.1304727110. Epub 2013 Nov 25.
CGL78 is a protein conserved in the green lineage and predicted to be located in the chloroplast. Member of the Ycf54 superfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Please, omitt SDS from transfer buffer and reduce transfer time to 45 min, Nitrocellulose membrane is recommended and SDS is omitted to allow this LMW protein to bind tighter to the membrane
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
24 kDa
Not reactive in:
Hordeum vulgare
Selected references:
Hsieh et al. (2013). The Proteome of Copper, Iron, Zinc, and Manganese Micronutrient Deficiency in Chlamydomonas reinhardtii. Mol Cell Proteomics. 2013 Jan;12(1):65-86. doi: 10.1074/mcp.M112.021840. Epub 2012 Oct 13.
Special application note:
Currently this antibody has not been confirmed to detect CGL78 protein in Arabidopsis thaliana. If you are interested to use this antibody in Arabidopsis, please, contact us.
COX11 (cytochrome c oxidase assembly protein) , involved in respiratory chain complex IV assembly.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardii
Expected Species:
Physcomitrium patensSpecies of your interest not listed? Contact us
Immunogen:
Recombinant fragment of Chlamydomonas reinhardtii COX11 UniProt: A8JCS0
PsaC is a conserved, chloroplast-encoded, Fe-S binding protein of approximately 10kDa, present in all known Photosystem I complexes. It is located on the stromal side of the thylacoid membranes. PsaC coordinates the Fe–S clusters FA and FB through two cysteine-rich domains.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
In some species minor cross reactions with some larger proteins are seen. These may contain related iron-sulfur binding motifs. Therefore size verification of the reacting band is required. Due to the small size of the protein, care should be taken to differentiate between chemiluminescent signal from PsaC and non-specific signals from chlotophylls or lipids if pigment is retained near the bottom of the blot.For the most optimal results use:thylakoid membranes or PSI particles, solubilized in a SDS sample buffer (final concentrations: 63 mM Tris HCl, 10% glycerol, 2% SDS, 0.0025% bromophenol blue) with 2.5% beta-mercaptoethanol at 85C for 2 minutes. The samples were spun softly, then the supernatant loaded. This product can be sold containing ProClin if requested.
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
9 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022). https://doi.org/10.1038/s41586-022-04662-9Ye et al. (2022) Effect of increased CO2 on iron-light-CO2 co-limitation of growth in a marine diatom, ASLO, Limnol. Oceanogr. 2022, 172-176Rogowski et al. (2021) Light as a substrate: migration of LHCII antennas in extended Michaelis-Menten model for PSI kinetics. J Photochem Photobiol B. 2021 Dec;225:112336. doi: 10.1016/j.jphotobiol.2021.112336. Epub 2021 Oct 19. PMID: 34736069.Levitan et al. (2019). Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17316-17322. doi: 10.1073/pnas.1906726116.Zavrel et al. (2019). Quantitative insights into the cyanobacterial cell economy. Elife. 2019 Feb 4;8. pii: e42508. doi: 10.7554/eLife.42508.
Special application note:
Peptide target used to elicit this antibody is well conserved in all photoautotrophs except some cyanobacteria, some red algae and Cyanophora paradoxa, which contain a conserved substitution of a valine to an isoleucine. The performance of the antibodies has been confirmed against taxa containing both the valine and isoleucine variants.Example of a simulataneous western blot detection with RbcL, PsbA and PsaC antibodies. More information about quantitative western blot using PsaC antibody can be found here.
Goat anti-human IgA (alpha chain) is a FITC conjugated secondary antibody which binds to human IgA (alpha chain). Antibody is specially adsorbed against bovine, mouse, rabbit serum. FITC, fluorescein-5-isothiocyanate, has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase human IgA.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the alpha chains on human IgA based on immunoelectrophoresis.Minimum cross-reactivity is observed to the light chains or non-IgA human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Rabbit anti-goat IgG (H&L), is a Rhodamine (TRITC) conjugated secondary antibody which binds to goat IgG (H&L) in immunological assays. Rhodamine - Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase goat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator, (ICC), immunohistochemistry ( IHC) (Flow cyt)
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Donkey anti-Rat IgG (H&L), DyLight 488 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rabbit or sheep IgG is a secondary antibody conjugated to DyLight 488, which binds to Rat IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG, light chains on all rat immunoglobulinsNo reactivity is observed to: non-immunoglobulin rat serum proteins, IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit or sheep
Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary, Rhodamine (TRITC-rhodamine - Tetramethylrhodamine-5-isothiocyanate) conjugated antibody, which is reacting against human IgG (H&L), F(ab)'2 fragment. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-rat IgG (H&L), is a secondary HRP (horse radish peroxidase) conjugated antibody which binds to rat IgGs in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Bui et al. (2020). Differential submergence tolerance between juvenile and adult Arabidopsis plants involves the ANAC017 transcription factor. doi.org/10.1101/2020.02.12.945923 BioRxiv.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins by immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Goat anti-mouse IgG (H&L) is a secondary, ALP (alkaline phosphatase) conjugated antibody which reacts with all mouse IgGs in immunological assays. Antibody is specially adsorbed against bovine,goat,human,rabbit and rat IgG and affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.
Donkey anti-Rat IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Rat IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rat IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Calvo-Polanco et al. (2014). Mild Salt Stress Conditions Induce Different Responses in Root Hydraulic Conductivity of Phaseolus vulgaris Over-Time. PLoS One. 2014 Mar 4;9(3):e90631. doi: 10.1371/journal.pone.0090631. eCollection 2014.
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG, light chains on all rat immunoglobulinsNo reactivity is observed to: non-immunoglobulin rat serum proteins
Donkey anti-mouse IgG (H&L) is a Rhodamine (TRITC) conjugated secondary antibody, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, sheep IgG. Rhodamine - Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-sheep IgG (H&L), is a biotin conjugated secondary antibody which is reacting with sheep IgG (H&L) in immunological assays. Antibody is specially adsorbed against human, mouse, rabbit IgG. Antibodies are affinity purified using solid phase sheep IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified donkey IgG.
Special application note:
This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins based on immunoelectrophoresis. Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Rabbit anti-mouse IgG (H&L) is a secondary antibody conjugated to Rhodamine (TRITC-rhodamine - Tetramethylrhodamine-5-isothiocyanate) which is reacting with mouse IgG (H&L) in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator, (ICC), immunohistochemistry ( IHC) (Flow cyt)
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgG Fc is a secondary antibody conjugated to Rhodamine (TRITC) which is reacting with human IgG Fc in immunological assays. Antibody is specially adsorbed against bovine, mouse, rabbit serum. Rhodamine (TRITC-rhodamine - Tetramethylrhodamine-5-isothiocyanate) has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase human IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified goat IgG.
Special application note:
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains on human immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin bovine, human, mouse or rabbit serum proteins, or mouse and rabbit IgG.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgM, chain, F(ab)'2 fragment is a HRP conjugated secondary antibody which is reacting with human IgM, chain, F(ab)'2. Min. cross-reactivity to human IgG or IgA. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on human IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed with IgG or IgA immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Rabbit anti-goat IgG (H&L) F(ab)'2 fragment is an unconjugated secondary antibody which is reacting with goat IgG (H&L) F(ab)'2 in immunological assays and is affinity purified. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-mouse IgG (H&L), F(ab)'2 fragment, is an unconjugated secondary antibody which is reacting with mouse IgG (H&L), F(ab)'2 fragment. Antibody is affinity purified. Min. cross-reactivity to human IgG/serum. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgG (H&L) is a FITC conjugated secondary antibody which is reacting with all mouse IgG (H&L) in immunological assays. Min. cross-reactivity to human IgG/serum. FITC (fluorescein-5-isothiocyanate) has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgG (H&L) is a secondary antibody HRP conjugated which binds to all mouse IgGs in immunological assays. It is specially adsorbed against bovine,goat,human,rabbit,rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator, Suggested starting dilution: 1 : 20-1 : 2000 depending upon the application (immunolocalization or western blot)
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins as well as bovine, goat, human, rabbit or rat IgG.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)
Goat anti-rabbit IgG (H&L) is a Rhodamine (TRITC - tetramethylrhodamine-5-isothiocyanate) conjugated secondary antibody, which binds to all rabbit IgGs in immunological assays. It has been specially adsorbed against bovine,goat,human,mouse and rat IgG. (TRITC) has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase rabbit IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator, (ICC), immunohistochemistry ( IHC) (Flow cyt)
Purity:
Immunogen affinity purified IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgG (H&L), F(ab)'2 fragment is a FITC conjugated secondary antibody which is reacting with mouse IgG (H&L), F(ab)'2 fragment. FITC (fluorescein-5-isothiocyanate) has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgM, chain is a HRP conjugated secondary antibody which binds to mouse IgM, chain in immunological assays. It has been specially adsorbed against human IgG/serum. Antibodies are affinity purified using solid phase mouse IgM.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on mouse IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins or to human IgG or serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Goat anti-mouse IgG Fc, (γ chain), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Mouse IgG Fc, (γ chain) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Mouse IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgGNo reactivity is observed to: non-immunoglobulin human serum proteins, light chains on all mouse immunoglobulins, F(ab)'2 fragment of mouse IgG
Goat anti-human IgM ( chain) is an ALP (alkaline phosphatase) conjugated secondary antibody, which binds to human IgM ( chain). Antibodies are affinity purified using solid phase human IgM.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with:heavy ( ) chains on human IgM based on immunoelectrophoresis, Minimum cross-reactivity is observed to: non-immunoglobulin serum proteins human IgG Antibody is supplied in 30 mM triethanolamine, pH 7,2, 5 mM magnesium chloride, 0,1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0,05 % (w/v) sodium azide as preservative
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
This antibody reacts with the heavy chains on hamsterIgG and with the light chains on all hamster immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin hamster serum proteins based on immunoelectrophoresis. Minimum cross-reactivity is observed to human serum based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Goat anti-mouse IgG Υ is a Rhodamine (TRITC) conjugated secondary antibody which binds to mouse IgG (heavy chain). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm, Emax = 570 nm. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on mouse IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-human IgG Fc is a FITC conjugated secondary antibody which binds to human IgG upsilon in immunological assays. FITC (fluorescein-5-isothiocyanate) has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase human IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on human IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains on human immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins. Minimum cross-reactivity is observed to human IgG, F(ab)'2 fragment based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Goat anti-human IgM ( chain), F(ab)'2 fragment is a biotin conjugated secondary antibody, which binds to human IgM ( chain), F(ab)'2 fragment in immunological assays. Min. cross-reactivity to human IgG or IgA. Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on human IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed with IgG or IgA immunoglobulins based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Goat anti-mouse IgG (H&L), F(ab)'2 fragment is a biotin conjugated secondary antibody which binds to mouse IgG (H&L) in immunological assays. Min. cross-reactivity to human IgG/serum. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins. No reactivity is observed with human IgG or serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Goat anti-rabbit IgG (H&L), F(ab)'2 fragment is an unconjugated secondary antibody which binds to rabbit IgG (H&L), F(ab)'2 fragment in immunological assays. The antibody is affinity purified, min. cross-reactivity to bovine, human, mouse IgG/serum. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins or to IgG from bovine, goat, human, mouse or rat based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified donkey IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgG Fc (heavy chain) is an unconjugated secondary antibody which binds to mouse IgG Fc. Antibodies are affinity purified using solid phase mouse IgG.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed with the light chains based on immunoelectrophoresis. Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative.
Goat anti-mouse IgG (H&L), is a secondary antibody biotin conjugated, which binds to all mouse IgGs in immunological assays. It is especially adsorbed against bovine,goat,human,rabbit,rat IgG and affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins. Minimum cross-reactivity was observed with IgG from bovine, goat, human, rabbit or rat based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative.
Goat anti-human IgM ( chain), F(ab)'2 fragment is a FITC conjugated secondary antibody which binds to human IgM ( chain), F(ab)'2 fragment in immunological assays. Fluorescein-5-isothiocyanate has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are affinity purified using solid phase human IgM.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the chains on human IgM based on immunoelectrophoresis.Minimum cross-reactivity is observed with IgG, F(ab)'2 fragment based on immunoelectrophoresis. No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Donkey anti-sheep IgG (H&L) is a HRP conjugated secondary antibody which binds to sheep IgG (H&L). It is especially adsorbed against human, mouse, rabbit IgG. Antibodies are affinity purified using solid phase sheep IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy chains on sheep IgG and with the light chains on all sheep immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin sheep serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-mouse IgG (H&L) is a biotinylated secondary antibody which binds to mouse IgG (H&L). Min. cross-reactivity to human IgG/serum. Antibodies are affinity purified using solid phase mouse IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Needs to be determined by end user for all immunoassay applications
Purity:
Immunogen affinity purified goat IgG.
Special application note:
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis, No reactivity is observed to non-immunoglobulin mouse serum proteins and human IgG or serum proteins based on immunoelectrophoresis
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
This antibody reacts with the heavy chains on goat IgG based on immunoelectrophoresis.Minimum cross-reactivity is observed to the light chains or to non- immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 0.05 % (w/v) sodium azide as preservative. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Rabbit anti-goat IgG (H&L), F(ab)'2 fragment is a biotinylated secondary antibody which binds to goat IgG (H&L), F(ab)'2 fragment Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Goat anti-mouse IgG (H&L), F(ab)'2 fragment is a HRP conjugated secondary antibody which binds to mouse IgG (H&L), F(ab)'2 fragment. Antibody purity is >90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store non-diluted antibody at 2-8°C. For storage at -20 °C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Rabbit anti-mouse IgG (H&L), DyLight 488 Conjugated is a secondary antibody conjugated to DyLight 488, which binds to Mouse IgG (H&L) in immunological assays.DyLight 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Mouse IgG (H&L)DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins
The retinoblastoma protein Rb is considered to be a key regulator of G1/S phase transition by blocking S phase entry and cell growth. Plant retinoblastoma-related (RBR) proteins share a homology with the human tumour suppressor retinoblastoma (pRb) protein. RBR protein functions are controlled by phosphorylation and protein-protein interactions. Short name: AtRBR
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C. Upon arrival Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Arabidopsis thaliana, Medicago sativa
Expected Species:
Camelia sinensis, Chenopodium rubrum, Cocos nucifera, Hordeum vulgare, Oryza sativa, Pisum sativum, Populus tremula, Scutelaria baicalensis, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
Recombinant C-terminal fragment consisting of 236 amino acids of Arabidopsis thaliana retinoblastoma protein UniProt: Q9LKZ3, lTAIR: At3g12280
Applications:
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western blot (WB)
This antibody is not suitable for immunolocalization.Methanol concentration in a transfer buffer can be considerably reduced or for a better transfer of high MW proteins (even with PVDF membrane).For immunoprecipitation start with 2 l and titrate it depending upon your experimental conditions. Please note that you work with a total IgY fraction, which means that it will contain between 40-60 g of total IgY (directed not only against retinoblastoma) therefore all of this IgY needs to be captured by the anti-IgY matrix.As control pre-serum for IP this product can be used, total, pre-immune IgY.
Application Details:
2 l (IP), 1 : 2000 (WB)
Purity:
Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
Molecular Weight:
112 kDa
Not reactive in:
Chlamydomonas reinhardtii
Selected references:
Leviczky et al. (2019). E2FA and E2FB transcription factors coordinate cell proliferation with seed maturation. Development. 2019 Nov 26;146(22). pii: dev179333. doi: 10.1242/dev.179333.Horvath et al. (2017). Arabidopsis RETINOBLASTOMA RELATED directly regulates DNA damage responses through functions beyond cell cycle control. EMBO J. 2017 May 2;36(9):1261-1278. doi: 10.15252/embj.201694561. Epub 2017 Mar 20.Cheng et al. (2013). Down-regulation of multiple CDK inhibitor ICK/KRP genes up-regulates E2F pathway and increases cell proliferation, organ and seed sizes in Arabidopsis. Plant j. May 7. brah m et al. (2011). Immunodetection of retinoblastoma-related protein and its phosphorylated form in interphase and mitotic alfalfa cells. J Exp Bot 62(6):2155-2168.
HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family and is involved in protein complex oligomerization and protein folding. Alternative names: AtHsp18.5, 18.5 kDa heat shock protein.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Hordeum vulgare
Expected Species:
Glycne max, Medicago truncatula, Medicago sativa, Pisum sativum, Ricinus communis, Rosa chinensis, Zea maysSpecies of your interest not listed? Contact us
Hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38 C for 3 hours at high humidity.Please, note that longer transfer time might result in losing a signal for this protein.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
18,5 kDa
Not reactive in:
Prosopis cineraria
Selected references:
Sadura et al. (2020). HSP Transcript and Protein Accumulation in Brassinosteroid Barley Mutants Acclimated to Low and High Temperatures . Int J Mol Sci . 2020 Mar 10;21(5):1889.doi: 10.3390/ijms21051889.
Special application note:
As hsp18,5 is a low abundancy protein, please, make sure that the plants are heated to the right temperature, Normally I heat stress the seedling on a sealed agar plate for 2 hours, This assures that the humidity around the plant is very high, Low humidity can allow the plant to cool down through transpiration, If a plant is in soil you can keep the leaf or even a whole plant over a wet filter paper and seal the plate very well during the treatment, Heat stressing plants in microphage tubes does not work that well
HSP90-2 (heat shock protein 90-2) is a constitutive isoform of HSP90, expressed in all tissues and abundant in root apical meristem, pollen and tapetum. Alternative names: ATHSP90.2, EARLY-RESPONSIVE TO DEHYDRATION 8, ERD8, HEAT SHOCK PROTEIN 90.2, HEAT SHOCK PROTEIN 81-2, HEAT SHOCK PROTEIN 81.2, HEAT SHOCK PROTEIN 90.2, HSP81-2, HSP81.2, HSP90.2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Sorghum bicolor
Expected Species:
Glycne max, Hordeum vulgare, Micromonas pulsilla, Nicotiana benthamina, Nicotiana tabacum, Ostreococcus lucimarinus, Oryza sativa, Physcomitrium patens, Populus balsamifera, Ricinus communis, Solanum tuberosum, Triticum aestivum, Zea mays, Vitis viniferaSpecies of your interest not listed? Contact us
Immunogen:
Full length recombinant HSP90-2 of Arabidopsis thaliana, UniProt: F4K6B6-1, TAIR: AT5G56030
This product can be sold containing ProClin if requested
Application Details:
1 : 3000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water
Molecular Weight:
80.6 | 95 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Gao et al. (2021) Identification of a bacterial-type ATP-binding cassette transporter implicated in aluminum tolerance in sweet sorghum (Sorghum bicolor L.). Plant Signal Behav. 2021 Jul 3;16(7):1916211. doi: 10.1080/15592324.2021.1916211. Epub 2021 May 26. PMID: 34034635; PMCID: PMC8205057.Barghetti et al. (2017). Heat-shock protein 40 is the key farnesylation target in meristem size control, abscisic acid signaling, and drought resistance. Genes Dev. 2017 Nov 15;31(22):2282-2295. doi: 10.1101/gad.301242.117.He et a. (2012).SpecificMissenseAlleles of theArabidopsisJasmonicAcidCo-ReceptorCOI1RegulateInnateImmuneReceptorAccumulation andFunction. PloS Genetics, Open Access.
Special application note:
Antibody reacts more with constitutive isofrom Hsp90-2 than heat inducible Hsp90-1
DELLA protein RGA is a putative transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. It is a member of VHIID/DELLA regulatory family and is the most sensitive to GA application, compare to other DELLA proteins.Involved in fruit and flower development. Synonymes: GAI-related sequence, GRAS family protein 10, AtGRAS-10, Repressor on the ga1-3 mutant, Restoration of growth on ammonia protein 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide chosen from RGA of Arabidopsis thaliana UniProt: Q9SLH3,TAIR: At2g01570
RGA protein is prone to degradation therefore caution has to be taken during protein extraction
Application Details:
1 : 1000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
64 | 64 kDa
Not reactive in:
Brassica napus, Populus sp., Rosa chinensis, Triticum aestivum
Selected references:
Dong et al. (2021). An HB40 - Jungbrunnen1 - GA 2-OXIDASE regulatory module for gibberellin homeostasis in ArabidopsisYan et al. (2020). FKF1 F-box Protein Promotes Flowering in Part by Negatively Regulating DELLA Protein Stability Under Long-Day Photoperiod in Arabidopsis. J Integr Plant Biol . 2020 May 19. doi: 10.1111/jipb.12971.Ferrero et al. (2019). Class I TCP transcription factors target the gibberellin biosynthesis gene GA20ox1 and the growth promoting genes HBI1 and PRE6 during thermomorphogenic growth in Arabidopsis. Plant Cell Physiol. 2019 Jul 11. pii: pcz137. doi: 10.1093/pcp/pcz137.Lorrai et al. (2018). Abscisic acid inhibits hypocotyl elongation acting on gibberellins, DELLA proteins and auxin. AoB Plants. 2018 Oct 5;10(5):ply061. doi: 10.1093/aobpla/ply061.Chahtane et al. (2018). The plant pathogen Pseudomonas aeruginosa triggers a DELLA-dependent seed germination arrest in Arabidopsis. Elife. 2018 Aug 28;7. pii: e37082. doi: 10.7554/eLife.37082.
GAI is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Less sensitive to GA. Synonymes:GRAS family protein 3, AtGRAS-3, Gibberelic acid-insensitive mutant protein, Restoration of growth on ammonia protein 2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
KLH-conugated synthetic peptide, chosen from Arabidopsis thaliana GAI protein sequence UniProt: Q9LQT8, TAIR: At1g14920
Homogenization with thiourea and bead beater. Protocol for protein extraction from seeds can be requested here. GAI protein is present in very low levels therefore specific material should be used for analysis as well as chemiluminescence detection reagent in extreme low femtogram range, as AgriseraECLSuperBright.
Application Details:
1 : 1000 (WB) on transfected Arabidopsis thaliana protoplasts
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 25 l of sterile water
Molecular Weight:
58,9 kDa
Not reactive in:
Brassica napus, Populus sp., Solanum lycopersicum
Selected references:
Gorshkova & Pojidaeva (2021). Members of the Universal Stress Protein Family are Indirectly Involved in Gibberellin-Dependent Regulation of Germination and Post-Germination Growth. Russ J Plant Physiol 68, 451 ??462 (2021). https://doi.org/10.1134/S1021443721030055Shahnejat-Bushehri et al. (2016). Arabidopsis NAC transcription factor JUB1 regulates GA/BR metabolism and signalling. NATURE PLANTS 2: Article number: 16013, 2016.
DMPO (5,5-dimethly-1-proline N-oxide) is a compound used in spin trapping. It is a method employed in chemistry as an analytical technique for detection and identification of short-lived free radicals. DMPO adducts can be generated with protein and DNA radicals.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C for one year, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
DMPO (species independent)
Expected Species:
DMPO (species independent)
Immunogen:
5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to ovalbumin
Applications:
ELISA (ELISA), Immunocytochemistry (ICC), Immuno-spin trapping (IT), Immunoprecipitation (IP), Western blot (WB)
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Chatterjee et al. (2009). Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice. Free Radic. Med. and Biol. 46:454-461.
Special application note:
Antibody is purified on Protein G and present in TBS pH 7.4 with 0.05 % sodium azide as preservative and 50 % glycerol.DMPO antibody in dilution of 1: 1000 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colormetric immunoblot analysis using goat anti-mouse IgG HRP conjugated secondary antibody.
EF1A (elongation factor 1-alpha) belongs to the GTP-binding elongation factor family and is localized in the cytoplasm. It is an essential enzyme in elongation phase of protein synthesis. There are four genes encoding EF1A in Arabidopsis, sequences of genes 1,2 and 3 are identical. Synonymes:eEF-1A.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Brassica napus, Nicotiana benthamiana, Picea sitchensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Zea maysSpecies of your interest not listed? Contact us
Immunogen:
Full length, recombinant EF1A of Arabidopsis thaliana UniProt: P0DH99-1, TAIR: AT1G07940
Markovi? et al. (2021)Correlation of elongation factor 1A accumulation with photosynthetic pigment content and yield in winter wheat varieties under heat stress conditions,Plant Physiology and Biochemistry,Volume 166,2021,Pages 572-581, ISSN 0981-9428,https://doi.org/10.1016/j.plaphy.2021.06.035.(https://www.sciencedirect.com/science/article/pii/S098194282100348X)Djuki? et al. (2019). Resolving subcellular plant metabolism. Plant J. 2019 Jul 30. doi: 10.1111/tpj.14472.Zhen et al. (2018). 2D-DIGE comparative proteomic analysis of developing wheat grains under high-nitrogen fertilization revealed key differentially accumulated proteins that promote storage protein and starch biosyntheses. Anal Bioanal Chem. 2018 Jul 30. doi: 10.1007/s00216-018-1230-4.Wang et al. (2016). GOLGI TRANSPORT 1B Regulates Protein Export from the Endoplasmic Reticulum in Rice Endosperm Cells. Plant Cell. 2016 Nov;28(11):2850-2865. (Oryza sativa, western blot)
Ergotamine is a secondary metabolite (natural product) and the principal alkaloid produced by the ergot fungus, Claviceps purpurea, and related fungi in the family Clavicipitaceae. It possesses structural similarity to several neurotransmitters, and has biological activity as a vasoconstrictor. It is used medicinally for treatment of acute migraine attacks (sometimes in combination with caffeine), and to induce childbirth and prevent post-partum haemorrhage.
Deoxynivalenol (DON) is a mycotoxin occuring in grains such as barley, maize, oats, rye, and wheat. It occurs less often in rice, sorghum, and triticale. The plant pathogens Fusarium graminearum (Gibberella zeae) and F. culmorum, which are causing Gibberella ear rot in maize and Fusarium head blight in wheat, are associated with the occurence of Deoxynivalenol. DON is a type B trichothecene, an epoxy-sesquiterpeneoid.Alternative name: Vomitoxin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cup to one month or in aliquots at -20 °C for long time storage. Avoid repeated freezing and thawing.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Ivanova et al. (2017). Role of P-glycoprotein in deoxynivalenol-mediated in vitro toxicity. Toxicol Lett. 2017 Nov 23;284:21-28. doi: 10.1016/j.toxlet.2017.11.021.
Special application note:
Antibodies are present in phosphate buffered saline, pH 7.2, 0.05% sodium azide as preservative
Aflatoxins are naturally occurring mycotoxins that are produced by many species of Aspergillus. Aflatoxins are toxic and among the most carcinogenic substances known. Crops which are frequently affected by Asperiillus include cereals (maize, sorghum, pearl millet, rice, wheat), oilseeds (peanut, soybean, sunflower, cotton), spices (chile peppers, black pepper, coriander, turmeric, ginger), and tree nuts (almond, pistachio, walnut, coconut, brazil nut).
Ochratoxin A, a toxin produced by Aspergillus ochraceus and Penicillium verrucosum, is one of the most abundant food-contaminating mycotoxins in the world. The toxin has been found in the tissues and organs of animals, including human blood and breast milk. Ochratoxin A can cause immunosuppression and immunotoxicity in animals.
Urtica dioica agglutinin (UDA), a monomeric lectin extracted from stinging nettle rhizomes, is specific for saccharides containing N-acetylglucosamine (GlcNAc). The lectin behaves as a superantigen for murine T cells, inducing the exclusive proliferation of lymphocytes. UDA is unique among known T cell superantigens because it can be presented by major histocompatibility complex (MHC) molecules of both class I and II. Beside that, Urtica lectin extracts are widely used for treatment of benign prostate hyperplasia.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
4°C up to one month or in aliquots at -20 °C for long time storage. Avoid repeated freezing and thawing.
Beta-CA1, beta-CA2 is a low-CO2-induced mitochondrial carbonic anhydrase. that catalyze the reversible interconversion of carbon dioxide and water to bicarbonate and protons. Localised into mitochondrial stroma.Alternative name: MtCA.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Chlamydomonas reinhardtii
Immunogen:
recombinant Chlamydomonas reinhardtii mitochondrial CA, as described in Villand et al. 1997. Accession number Q39590 and Q39589
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Burlacot et al. (2022) Alternative photosynthesis pathways drive the algal CO2-concentrating mechanism. Nature 605, 366–371 (2022). https://doi.org/10.1038/s41586-022-04662-9Kuken et al. (2018). Effects of microcompartmentation on flux distribution and metabolic pools in Chlamydomonas reinhardtii chloroplasts. Elife. 2018 Oct 11;7. pii: e37960. doi: 10.7554/eLife.37960.Muranaka et al. (2015). TEF30 interacts with photosystem II monomers and is involved in the repair of photodamaged photosystem II in Chlamydomonas reinhardtii. Plant Physiol. 2015 Dec 7. pii: pp.01458.2015.Tirumani et al. (2014). Regulation of CCM genes in Chlamydomonas reinhardtii during conditions of light-dark cycles in synchronous cultures. Plant Mol Biol. 2014 Mar 4.Renberg et al. (2010). A Metabolomic Approach to Study Major Metabolite Changes during Acclimation to Limiting CO2 in Chlamydomonas reinhardtii. Plant physiol. 154: 187-196.
Special application note:
Antibody is recognizing both isoforms, beta- CA1 and beta-CA2 and can be used as mitochondrial marker for low carbon dioxide grown cells of Chlamydomonas reinhardtii
Rpl1 (50S ribosomal protein L1) belongs to the ribosomal protein L1P family. This protein binds directly to 23S rRNA, acts also as a translational repressor protein.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Linhartov et al. (2014). Accumulation of the Type IV prepilin triggers degradation of SecY and YidC and inhibits synthesis of Photosystem II proteins in the cyanobacterium Synechocystis PCC 6803. Mol Microbiol. 2014 Jul 24. doi: 10.1111/mmi.12730.
ADP-glucose pyrophosphorylase (AGPase) catalyses the first committed step in the synthesis of transient starch in chloroplasts and storage starch in amyloplasts in plants (Tetlow et al., 2004). AGPase in higher plants is composed of two distinct subunits encoded by separate genes, forming an L2S2 heterotetramer. The large subunits (L) are modulators of allosteric activity, while the small subunits (S) are the catalytic subunits (Kim et al., 2007). Synonymes:ADP-glucose pyrophosphorylase, ADP-glucose synthase, AGPase B, Alpha-D-glucose-1-phosphate adenyl transferase
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Cross reactivity of this antibody to Rubisco has been excluded using 2D gel electrophoresis.Antigen used to elicit this antbody has very poor conservation in large subunit of ADP-glucose pyrophosphorylase.
Application Details:
1 : 1000-1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
49,4 | 52 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Lim et al (2022). Arabidopsis guard cell chloroplasts import cytosolic ATP for starch turnover and stomatal opening. Nat Commun. 2022 Feb 3;13(1):652. doi: 10.1038/s41467-022-28263-2. PMID: 35115512; PMCID: PMC8814037.Ma et al. (2021) A plasma membrane transporter coordinates phosphate reallocation and grain filling in cereals. Nat Genet. 2021 Apr 29. doi: 10.1038/s41588-021-00855-6. Epub ahead of print. PMID: 33927398.Chang et al. (2020). Enhanced lipid productivity in AGP knockout marine microalga Tetraselmis sp. using a DNA-free CRISPR-Cas9 RNP method. Bioresource Technology.Volume 303, May 2020, 122932. Ancin et al. (2019). NTRC and Thioredoxin f Overexpression Differentially Induces Starch Accumulation in Tobacco Leaves.Plants (Basel). 2019 Nov 26;8(12). pii: E543. doi: 10.3390/plants8120543.Takahashi et al. (2019). Glutelin subtype-dependent protein localization in rice grain evidenced by immunodetection analyses. Plant Mol Biol. 2019 Mar 25. doi: 10.1007/s11103-019-00855-5.
Rabbit anti-hamster IgG (H&L) is a secondary antibody which binds to hamster IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase hamster IgG (H&L).
This antibody is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is > 4.5 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on hamster, IgG light chains on all hamster immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin hamster serum proteins
Rabbit anti-hamster IgG (H&L) is a secondary antibody conjugated to ALP, which binds to hamster IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase hamster IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified rabbit IgG.
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on hamster IgG, light chains on allhamster immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin hamster serum proteins.
Rabbit anti-hamster IgG (H&L) is a secondary antibody conjugated to HRP, which binds tohamster IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase hamster IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator,
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on hamster IgG, light chains on all hamster immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin hamster serum proteins
DHAR1 ( Dehydroascorbate Reductase 1) the protein is induced by jasmonic acid and oxidative chemical stresses and is a key component of the ascorbate recycling system. Involved in redox homeostasis under biotic and abiotic inducers. Localized in mitochondria. Synonymes: glutathione-dependent dehydroascorbate reductase 1, chloride intracellular channel homolog 1, CLIC homolog 1, glutathione-dependent dehydroascorbate reductase 1, AtDHAR1, GSH-dependent dehydroascorbate reductase 1, mtDHAR, AT1G19570.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Nicotiana tabacum, Populus trichocarpa, Ricinus communis, Solanum tuberosum, Triticum aestivum, Zinnia elegans Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known DHAR1 sequence of Arabidopsis thaliana Q9FWR4, At1g19570
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Szymańska et al. (2019). SNF1-Related Protein Kinases SnRK2.4 and SnRK2.10 Modulate ROS Homeostasis in Plant Response to Salt Stress. Int J Mol Sci. 2019 Jan 2;20(1). pii: E143. doi: 10.3390/ijms20010143.Witzel et al. (2017). Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome. J Proteomics. 2017 Oct 3;169:215-224. doi: 10.1016/j.jprot.2017.04.008.Wang et al. (2014). Proteomic analysis of salt-responsive proteins in the leaves of mangrove Kandelia candel during short-term stress. PLoS One. 2014 Jan 8;9(1):e83141. doi: 10.1371/journal.pone.0083141. eCollection 2014.
DHAR2 ( Dehydroascorbate Reductase 2) the protein is induced by jasmonic acid and oxidative chemical stresses and is a key component of the ascorbate recycling system. Involved in redox homeostasis under biotic and abiotic inducers. Localized in cytoplasm. Synonymes: chloride intracellular channel homolog 2, CLIC homolog 2, glutathione-dependent dehydroascorbate reductase 2, DHAR2, CytDHAR, GSH-dependent dehydroascorbate reductase 2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Ricinus communis, Populus trichocarpa Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known DHAR1 sequence of Arabidopsis thaliana Q9FRL8, At1g75270
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Grefen et al. (2009). The determination of protein-protein interactions by the mating-based split-ubiquitin system (mbSUS). Methods Mol Biol 479:217-233.
AAA2 domain is present in ATP binding proteins involved in stress response and being members of clpA/clpB family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Protease Do-like 7 (DegP7) EC=3.4.21 is a serine/cysteine protease involved in photoiunhibition process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica oleracea
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana DegP7 UniProt: Q8RY22-1 , TAIR: At3g03380
Antibody will provide a good signal on enriched nuclei fractions but not on total cell extracts
Application Details:
1 : 500 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Molecular Weight:
119,8 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Schuhmann et al. (2011). A new principle of oligomerization of plant DEG7 protease based on interactions of degenerated protease domains. Biochem. J. 435:167-174.
Ferredoxins are soluble, iron-sulfur containg proteins that function as electron donors in many metabolic pathways. Ferredoxin is also a target for thioredoxin action.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Terrauchi et al. (2009). Pattern of expression and substrate specificity of chloroplast ferredoxins from Chlamydomonas reinhardtii. J Biol Chem 284 (38):25867-25878.
Special application note:
This product can be sold containing ProClin if requested
WUSCHEL is a transcription factor which plays a central role during early embryogenesis, oogenesis, flowering.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana WUSCHEL protein sequence, UniProt: Q9SB92, TAIR:At2g17950
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Dory et al. (2016). Kinase-Associated Phosphoisoform Assay: a novel candidate-based method to detect specific kinase-substrate phosphorylation interactions in vivo. BMC Plant Biol. 2016 Sep 21;16(1):204.
Homeobox protein SHOOT MERISTEMLESS is a protein required for shoot apical meristem (SAM) formation during embryogenesis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus
Immunogen:
KLH-conjugated peptide chosen from Arabidopsis thaliana STM protein sequence, UniProt: Q38874,TAIR: At1g62360
Sub1C is a protein which is involved in rice tolerance to submergence.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Oryza sativa
Expected Species:
Oryza sativa
Immunogen:
KLH-conjugated synthetic peptide derived from Oryza sativa Sub1C. Chosen peptide is convered in all 6 isoforms (C-1 to C-6).
His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20 °C.
Host Animal:
Mouse
Species Reactivity:
6xHis
Expected Species:
6xHis
Immunogen:
KLH-conjugated synthetic peptide 6xHis
Applications:
Dot Blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunlocalization (IL), Western blot (WB)
Antibody is present in 10 mM PBS, pH 7.2His-tag (6,8,10xHis) needs to be properly exposed to allow detection. To prevent target protein folding, extraction should be performed with 6 to 8 M urea or using TCA-acetone precipitation method.
Application Details:
1 : 1000 (WB)
Conjugation:
IgG2b
Isotype:
IgG2b
Purity:
Total IgG fraction. Protein A purified.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
De Brasi-Velasco et al. (2021). Autophagy Is Involved in the Viability of Overexpressing Thioredoxin o1 Tobacco BY-2 Cells under Oxidative Conditions. Antioxidants. 2021; 10(12):1884. https://doi.org/10.3390/antiox10121884Tan et al. (2020). Salicylic Acid Targets Protein Phosphatase 2A to Attenuate Growth in Plants. Curr Biol. 2020 Feb 3;30(3):381-395.e8. doi: 10.1016/j.cub.2019.11.058.L pez-Vidal et al. (2020). Is Autophagy Involved in Pepper Fruit Ripening? Cells, 9 (1) , DOI: 10.3390/cells9010106 H ggmark-M nberg et al. (2016). Autoantibody targets in vaccine-associated narcolepsy. Autoimmunity. 2016 Sep;49(6):421-433. Epub 2016 May 20.
Special application note:
Working dilution for ELISA, IL and IP needs to be determined experimentally
Goat anti-mouse IgG (H&L) is a secondary antibody conjugated to HRP, which binds to mouse IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase mouse IgG (H&L).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Heavy chains on mouse IgG and light chains on all mouse immunoglobulins
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily.
Not reactive in:
Non-immunoglobulin mouse serum proteins
Selected references:
Kasari et al. (2019). A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling. Nucleic Acids Res. 2019 Jul 12. pii: gkz600. doi: 10.1093/nar/gkz600.Li and Bock (2018). Replication of bacterial plasmids in the nucleus of the red alga Porphyridium purpureum. Nat Commun. 2018 Aug 27;9(1):3451. doi: 10.1038/s41467-018-05651-1.Shin et al. (2017), Complementation of a mutation in CpSRP43 causing partial truncation of light-harvesting chlorophyll antenna in Chlorella vulgaris. Sci Rep. 2017 Dec 20;7(1):17929. doi: 10.1038/s41598-017-18221-0.Dmitrović et al. (2015). Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro. Acta Physiologiae Plantarum, February 2015, 37:64.
Special application note:
Concentration: 1.0 mg/ml. Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) ProClin 150 as preservative of bacterial growth.The antibody will detect all isotypes of mouse IgG.
YFP (Yellow Fluorescent Protein) is a genetic mutant of green fluorescent protein (GFP). YFP has an excitation peak at 514 nm and emission peak at 527 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
C-YFP tagged proteins from Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from C-terminal of YFP protein. This peptide is conserved in pGWB541 Vector.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Li et al. (2021) Two ubiquitin-associated ER proteins interact with COPT copper transporters and modulate their accumulation, Plant Physiology, 2021;, kiab381, https://doi.org/10.1093/plphys/kiab381Lung et al. (2021) Oxylipin signaling in salt-stressed soybean is modulated by ligand-dependent interaction of Class II acyl-CoA-binding proteins with lipoxygenase. Plant Cell. 2021 Dec 17:koab306. doi: 10.1093/plcell/koab306. Epub ahead of print. PMID: 34919703.
YFP (Yellow Fluorescent Protein) is a genetic mutant of green fluorescent protein (GFP). YFP has an excitation peak at 514 nm and emission peak at 527 nm.This antibody is directly conjugated to ALP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
C-YFP tagged proteins from Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from C-terminal of YFP protein. This peptide is conserved in pGWB541 Vector.
YFP (Yellow Fluorescent Protein) is a genetic mutant of green fluorescent protein (GFP). YFP has an excitation peak at 514 nm and emission peak at 527 nm.This antibody is directly conjugated to Biotin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
C-YFP tagged proteins from Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from C-terminal of YFP protein. This peptide is conserved in pGWB541 Vector.
YFP (Yellow Fluorescent Protein) is a genetic mutant of green fluorescent protein (GFP), YFP has an excitation peak at 514 nm and emission peak at 527 nm. This antibody is directly conjugated to HRP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
C-YFP tagged proteins from Arabidopsis thaliana
Expected Species:
C-YFP tagged proteins
Immunogen:
KLH-conjugated synthetic peptide derived from C-terminal of YFP protein. This peptide is conserved in pGWB541 Vector.
YFP (Yellow Fluorescent Protein) is a genetic mutant of green fluorescent protein (GFP), YFP has an excitation peak at 514 nm and emission peak at 527 nm. This antibody is directly conjugated to HRP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
C-YFP tagged proteins from Arabidopsis thaliana
Expected Species:
C-YFP tagged proteins
Immunogen:
KLH-conjugated synthetic peptide derived from C-terminal of YFP protein. This peptide is conserved in pGWB541 Vector.
YFP (Yellow Fluorescent Protein) is a genetic mutant of green fluorescent protein (GFP), YFP has an excitation peak at 514 nm and emission peak at 527 nm.This antibody is directly conjugated to HRP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS pH 7,4.
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Store in provided dark tube and avoid direct light exposure. Shortly spin the tube before use.
Host Animal:
Rabbit
Species Reactivity:
C-YFP tagged proteins from Arabidopsis thaliana
Expected Species:
C-YFP tagged proteins
Immunogen:
KLH-conjugated synthetic peptide derived from C-terminal of YFP protein. This peptide is conserved in pGWB541 Vector.
YFP (Yellow Fluorescent Protein) is a genetic mutant of green fluorescent protein (GFP). YFP has an excitation peak at 514 nm and emission peak at 527 nm.This antibody is directly conjugated to HRP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°Cfor 12-18 months. A preservative may be added for long time storage up to 2 years. Avoid sodium azide.
Host Animal:
Rabbit
Species Reactivity:
C-YFP tagged proteins from Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from C-terminal of YFP protein. This peptide is conserved in pGWB541 Vector.
YFP (Yellow Fluorescent Protein) is a genetic mutant of green fluorescent protein (GFP). YFP has an excitation peak at 514 nm and emission peak at 527 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
N-YFP tagged proteins from Arabidopsis thaliana, Escherichia coli, Nicotiana tabacum
Immunogen:
KLH-conjugated synthetic peptide derived from N-terminal of YFP protein.
YFP molecular weight is 27 kDa, however detected band is going to have MW increased by a fused protein
Application Details:
1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Li et al. (2021) Two ubiquitin-associated ER proteins interact with COPT copper transporters and modulate their accumulation, Plant Physiology, 2021;, kiab381, https://doi.org/10.1093/plphys/kiab381Lung et al. (2021) Oxylipin signaling in salt-stressed soybean is modulated by ligand-dependent interaction of Class II acyl-CoA-binding proteins with lipoxygenase. Plant Cell. 2021 Dec 17:koab306. doi: 10.1093/plcell/koab306. Epub ahead of print. PMID: 34919703.Schultz-Larsen et al. (2018). The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity. Cell Rep. 2018 Nov 27;25(9):2329-2338.e5. doi: 10.1016/j.celrep.2018.11.011.
IRT1 is a high afinity iron transported that plays a key role in the uptake of iron (in rhizosphere across the plasma membrane in the root epidermal layer) as well as mediates the heavy metals uptake under iron-defficiency. Is a principal regulator of iron homeaostasis in plants. Synonymes:Fe(2+) transport protein 1, Fe(II) transport protein 1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, This antibody can be stored in a solution containg 50 % glycerol, final concentration, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Brassica napus, Brassica oleracea, Camelina sativa, Capsella rubella, Noccaea caerulescens, Thlaspi cerulescensSpecies of your interest not listed? Contact us
Gautam et al. (2021) IRONMAN Tunes Responses to Iron Deficiency in Concert with Environmental pH. bioRxiv 2021.02.16.431461; doi: https://doi.org/10.1101/2021.02.16.431461Ivanov et al. (2014). SORTING NEXIN1 Is Required for Modulating the Trafficking and Stability of the Arabidopsis IRON-REGULATED TRANSPORTER1. Plant Cell. 2014 Mar 4.Selote et al. (2014). Iron-binding E3 ligase mediates iron response in plants by targeting bHLH transcription factors. Plant Physiol. 2014 Dec 1. pii: pp.114.250837.
Goat anti-rabbit IgG (H&L), DyLight 550 conjugated, is a secondary antibody which binds to rabbit IgGs in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Fluor to protein ratio is 2.47 moles DyLight 550 per mole antibody. Antibodies are adsorbed against bovine,goat,human,mouse,rat IgG and are affinity purified using solid phase rabbit IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
This antibody reacts with the heavy (gamma) chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rabbit serum proteins and bovine,goat,human,mouse,rat IgG based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Acd1 (accelerated cell death 1) EC=1.14.12.20 is an enzyme involved in chlorophyll breakdown, pheophorbide a oxygenase. This enzyme seems to induce cell death in Arabidopsis leaves in the dark. Synonymes:PaO, Lls1, lethal leaf-spot 1 homolog, pheide a oxygenase
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus, Solanum lycopersicum, Nicotiana tabacum Species of your interest not listed? Contact us
Immunogen:
Recombinant PaO from Arabidopsis thaliana Q9FYC2, At3g44880
This antibody works on total cell extracts and can be used as a senescence marker. Predicted size of Acd1 precursor protein is about 61 kD including the transit peptide, but it must be processed to a smaller size. Using fresh extracts is recommended to decrease possible cross-reaction with Rubisco.
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
61 | 54 kDa
Not reactive in:
Pinus strobus
Selected references:
Fukura et al. (2021) Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions. J Plant Physiol. 2021 Nov;266:153535. doi: 10.1016/j.jplph.2021.153535. Epub 2021 Sep 25. PMID: 34607178.Kim et al. (2013). Mutation of the Arabidopsis NAC016 Transcription Factor Delays Leaf Senescence.'Plant Cell Physiol. Aug 21.Nagane et al. (2010). Involvement of AtNAP1 in thre reulation of chlorophyll degradation in Arabiopsis thaliana. Planta (4):939-949.Hirashima et al. (2009). Light-independent cell death induced by accumulation of pheophorbide a in Arabidopsis thaliana. Plant Cell Physiol. (4):719-729.
Special application note:
The protein level is moderately induced during dark-induced senescence
The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Algae (brown and red), Conifers, Cyanobacteria, Brassica napus, Diatoms, Glycine max, Manihot esculenta, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Pisum sativum, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from N-terminal of available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others
This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel
Application Details:
1 : 1000-1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
38 | 28-30 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Chen et al. (2021)Degradation of the photosystem II core complex is independent of chlorophyll degradation mediated by Stay-Green Mg2+ dechelatase in Arabidopsis,Plant Science,Volume 307,2021,110902,ISSN 0168-9452,https://doi.org/10.1016/j.plantsci.2021.110902. Fukura et al. (2021) Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions. J Plant Physiol. 2021 Nov;266:153535. doi: 10.1016/j.jplph.2021.153535. Epub 2021 Sep 25. PMID: 34607178.Terentyev (2020: The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated From Wild Type and cia3 Mutant Chlamydomonas reinhardtii. Life (Basel). 2020 May 14;10(5):E63. doi: 10.3390/life10050063..G recka et al. (2019). Photosystem II 22kDa protein level a prerequisite for excess light-inducible memory, cross-tolerance to UV-C, and regulation of electrical signalling. Plant Cell Environ. 2019 Nov 23. doi: 10.1111/pce.13686.Liu et al. (2018). Effects of PSII Manganese-Stabilizing Protein Succinylation on Photosynthesis in the Model Cyanobacterium Synechococcus sp. PCC 7002. Plant Cell Physiol. 2018 Jul 1;59(7):1466-1482. doi: 10.1093/pcp/pcy080.
Special application note:
Peptide target used for antibody production comes from Helix 1 of PSII, lumenal exposed loop. Antibodies are going to recognize the target in a wide range of species.This product can be sold containing ProClin if requested.
PsbC (CP43) acts as an antenna to the PSII core and its presence seem to be also necessary for maintaining water splitting activity. This protein is more weakly associated with the PSII reaction centre and can be removed from the isolated core.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
In C4 plants like Echinochloa crus-galli and Zea mays antibody detects 2 bands.
Application Details:
1 : 3 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
45 | 43 kDa
Not reactive in:
diatoms
Selected references:
Beckova et al. (2022). Photosystem II antenna modules CP43 and CP47 do not form a stable 'no reaction centre complex' in the cyanobacterium Synechocystis sp. PCC 6803. Photosynth Res. 2022 Jan 11. doi: 10.1007/s11120-022-00896-w. Epub ahead of print. PMID: 35015206.Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii. Plant J. 2021 Jul 4. doi: 10.1111/tpj.15405. Epub ahead of print. PMID: 34218480.Okegawa et al (2021) Maintaining the Chloroplast Redox Balance Through the PGR5-Dependent Pathway and the Trx System is Required for Light-Dependent Activation of Photosynthetic Reactions. Plant Cell Physiol. 2021 Oct 8:pcab148. doi: 10.1093/pcp/pcab148. Epub ahead of print. PMID: 34623443.Sakuraba at al. (2020). Multilayered regulation of membrane-bound ONAC054 is essential for abscisic acid-induced leaf senescence in rice. Plant Cell. 2020 Jan 6. pii: tpc.00569.2019. doi: 10.1105/tpc.19.00569.Dong et al. (2020). Plastid ribosomal protein LPE2 is involved in photosynthesis and the response to C/N balance in Arabidopsis thaliana. J Integr Plant Biol. 2020 Jan 15. doi: 10.1111/jipb.12907.
FtsH belong to a family of ATP dependent peptidases. Localized in a chloroplast are following isoforms: FTSH1 (synonymes AAA, FTSH, FTSH Protease 1), Ftsh2 (VAR2, VARIEGATED 2), FtsH5 (VAR1, VARIEGATED 1), FtsH6 (FTSH PROTEASE 6), FtsH7, FtsH8. FtsH9.Localized in mitochondria are following isoforms: FtsH3 (FTSH Protease 3), FtsH4, FtsH10, FtsH11.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Diatoms including Phaeodactylum tricornutum, dicots, Chlamydomonas reinhardtii, Monocots; Nannochloropsis gaditana, Solanum lycopersicumSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide dereived from sequences of all known FtsH isoforms of Arabidopsis thaliana: FtsH1 Q39102 (At1g50250), FtsH2 O80860 (At2g30950), FtsH3 Q84WU8 (At2g29080), FtsH4 O80983 (At2g26140), FtsH5 Q9FH02 (At5g42270), FtsH6 Q1PDW5 (At5g15250), FtsH7 Q9SD67 (At3g47060), FtsH8 Q8W585 (At1g06430), FtsH9 Q9FIM2 (At5g58870), FtsH10 Q8VZI8 (At1g07510), FtsH11 Q9FGM0 (At5g53170) as well as 4 FtsH isoforms of Synechocystis sp. PCC6803NP_440330.1, NP_442160.1, NP_440797.1, NP_440525.1
For detection on a diatom samples, load of 5-10 g/well is required as well as usage of enchanced chemiluminescence.The antibody also detects the chloroplast-encoded FtsH from Thalassiosira pseudonana (diatom) along with 3 related isoforms encode in the nuclear genome of Thalassiosira pseudonana.For detection in BlueNative, recommened dilution is 1: 500.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Dogra et al. (2019). Oxidative post-translational modification of EXECUTER1 is required for singlet oxygen sensing in plastids. Nat Commun. 2019 Jun 27;10(1):2834. doi: 10.1038/s41467-019-10760-6. Li et al. (2016). A Hard Day's Night: Diatoms Continue Recycling Photosystem II in the Dark. Front. Mar. Sci., 08 November 2016Tietz et al. (2015). Functional Implications of Photosystem II Crystal Formation in Photosynthetic Membranes. J Biol Chem. 2015 Apr 20. pii: jbc.M114.619841.Campbell et al. (2013). Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana. Photosynth. Res. March 16.
FtsH belong to a family of ATP dependent peptidases. Localized in a chloroplast are following isoforms: FTSH1 (synonymes AAA, FTSH, FTSH Protease 1), Ftsh2 (VAR2, VARIEGATED 2), FtsH5 (VAR1, VARIEGATED 1), FtsH6 (FTSH PROTEASE 6), FtsH7, FtsH8. FtsH9.Localized in mitochondria are following isoforms: FtsH3 (FTSH Protease 3), FtsH4, FtsH10, FtsH11.
Product Type:
Antibody
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Concentration: after adding 225 l of sterile milliQ water final concentration of the standard is 0.1 pmoles/ lProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap. This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra careto mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing,
Application Details:
Standard curve: 3 loads are recommended (0.5, 2 and 4μl).For most applications a sample load of 0.2μg of chlorophyll will give a FtsH2 signal in this range.Positive control: load per well: a 2 μl load is optimal for most chemiluminescent detection systems.Standard curve: 3 loads are recommended (0.5, 2 and 4 l).For most applications a sample load of 0.2 g of chlorophyll will give a FtsH2 signal in this range.Positive control: a 2 l load per well is optimal for most chemiluminescent detection systems.Non-disulphie dependent dimers and complexes can be also detected using standard western blot methods withmore sensitive detection reagents as ECL Advance or West Pico when loading per well more standard than recommended. They have not been included in the standard calibration.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:
For reconstitution add 225 l of sterile water
Molecular Weight:
75 kDa
Selected references:
Li et al. (2016). A Hard Day's Night: Diatoms Continue Recycling Photosystem II in the Dark. Front. Mar. Sci., 08 November 2016
Special application note:
The FtsH2 protein standard can be used in combination with anti-FtsH2 antibodies AS11 1789 to quantitate FtsH2 from a range of cyanobacteria. Global antibodies are raised against highly conserved amino acid sequences in the FtsH protein.Quantitative western blot: detailed method description, video tutorial
HDT1 (HD2A) is probably mediating in the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in cell cycle progression, transcriptional regulation and developmental events. This protein is also involved in rRNA gene silencing in nucleolar dominance. Synonymes:HD-tuins protein 1, Histone deacetylase 2a.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Expected Species:
Arabidopsis thaliana
Immunogen:
Recombinant part of Arabidopsis thaliana HDT1 Q9FVE6, At3g44750
Derbyshire et al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314.
Special application note:
Antibody is present in PBS + 50 % glycerol and 0,01 % of sodium azide as preservative of bacterial growth
Hydroxypyruvate reductase (HPR) belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family (oxidoreductases) and is involved in glycine, serine and theronine and glyoxylate and dicarboxylate metabolism. Synonymes: HPR, beta-hydroxypyruvate reductase, NADH:hydroxypyruvate reductase, D-glycerate dehydrogenase.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Pisum sativum
Expected Species:
Chlamydomonas reinhardtii, Chlorella sp. ,Cucumis sativus, Glycine max, Oryza sativa, Populus albaxtremula, Ricinus communis, VolvoxSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known plant HRP sequences, including Arabidopsis thaliana UniProt: Q9C9W5,TAIR: At1g68010
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Bapatla et al. (2021). Modulation of Photorespiratory Enzymes by Oxidative and Photo-Oxidative Stress Induced by Menadione in Leaves of Pea (Pisum sativum). Plants 10, no. 5: 987. https://doi.org/10.3390/plants10050987Korotaeva et al. (2018). Effect of Heat Hardening on Expression of Genes phb3 and phb4 and Accumulation of Phb Proteins in Green Leaves of Arabidopsis thaliana. Russian Journal of Plant Physiology, 65(5), 688-696, 2018 https://doi.org/10.1134/s1021443718040039Farmer et al. (2013).Disrupting Autophagy Restores Peroxisome Function to an Arabidopsis lon2 Mutant and Reveals a Role for the LON2 Protease in Peroxisomal Matrix Protein Degradation. Plant Cell, Oct 31.
PEL1 (Pelota) is required for normal chromosome segregation during cell division and genomic stability. Synonymes: Eukaryotic release factor 1 (ERF1) family protein, Putative pelota (PEL1) protein
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Oryza sativa, Ostreococcus tauri, Ricinus communis, Populus canadensis, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known plant Pelota sequences including Arabidopsis thaliana UniProt: Q9ZT87 TAIR: At4g27650
Jasmonic acid (JA) is a member of the jasmonate class of plant hormones and is derived from the fatty acid linolenic acid, biosynthesized from linolenic acid by the octadecanoid pathway. Its main function is regulation of plant response to abiotic and biotic stresses as well as plant growth and development.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Populus trichocarpa, Solanum lycopersicum
Expected Species:
Higher Plants Species of your interest not listed? Contact us
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Wojciechowska et al. (2020). Abscisic Acid and Jasmonate Metabolisms Are Jointly Regulated During Senescence in Roots and Leaves of Populus trichocarpa. Int J Mol Sci , 21 (6)
Special application note:
This product can be sold containing ProClin if requested
ACC (1-Aminocyclopropane-1-carboxylic acid) plays an important role in the biosynthesis of the plant hormone ethylene. It is synthesized by the enzyme ACC synthase ( EC 4.4.1.14) from methionine and converted to ethylene by ACC oxidase (EC 1.14.17.4).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Details of used immunogold protocol are provided under an image
Application Details:
1 : 100 (IG)
Purity:
Serum
Reconstitution:
For reconstitution add 200 l of sterile water
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Wilmowicz et al. (2019). Abscisic acid and ethylene in the control of nodule-specific response on drought in yellow lupine. Environmental and Experimental Botany Available online 2 October 2019, 103900.Serova et al. (2018). Early nodule senescence is activated in symbiotic mutants of pea (Pisum sativum L.) forming ineffective nodules blocked at different nodule developmental stages. Protoplasma. 2018 Apr 3. doi: 10.1007/s00709-018-1246-9.
Histone 1 (H1) is a protein located in nuclei, incorporated into chromatin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Rutowicz et al. (2019). Linker histones are fine-scale chromatin architects modulating developmental decisions in Arabidopsis. Genome Biol. 2019 Aug 7;20(1):157. doi: 10.1186/s13059-019-1767-3. (western blot, immunolocalization)Benoit et al. (2018). Replication-coupled histone H3.1 deposition determines nucleosome composition and heterochromatin dynamics during Arabidopsis seedling development. New Phytol. 2018 Jun 13. doi: 10.1111/nph.15248.Wollmann et al. (2017). The histone H3 variant H3.3 regulates gene body DNA methylation in Arabidopsis thaliana. Genome Biol. 2017 May 18;18(1):94. doi: 10.1186/s13059-017-1221-3.She and Baroux (2015). Chromatin dynamics in Pollen Mother Cells underpin a common scenario at the somatic-to-reproductive fate transition of both the male and female lineages in Arabidopsis. Front. Plant Sci. | doi: 10.3389/fpls.2015.00294.She et al. (2013). Chromatin reprogramming during the somatic to-reproductive cell fate transition in plants. Development Oct;140(19):4008-19. doi: 10.1242/dev.095034. Epub 2013 Sep 4. (Arabidopsis thaliana, immunostaining)
DELLA protein RGL2 is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Involved in the biosynthesis of abscisic acid (ABA), delays flowering and leaf production, reulates floral development, petal, stamen and anther development. Predominantly expressed in germinating seeds, flowers and siliques. Synonymes: GRAS family protein 15, AtGRAS-15, Scarecrow-like protein 19, AtSCL19.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
As this protein is expressed in tissue in very low levels, please load at least 50 g/well.Recommended extraction and denaturation protocol: Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 l of 2.5 x Laemli for 80-120 mg of homogenized material) and boiled for 2 min. at 95 C in 2,5x Laemmli Buffer (with 60 Mm DTT final concentration) otherwise RGA protein will degrade.
DNA-directed RNA polymerase II subunit 1 (EC=2.7.7.6) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This enzyme is the central component of the basal RNA polymerase II transcription machinery. Synonymes:DNA-directed RNA polymerase III largest subunit,DNA-directed RNA polymerase II subunit RPB1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Amygdalus persica, Asparagus officinalis, Brassica rapa, Capsella rubella, Glycine max, Hordeum vulgare, Solanum lycopersicum, Oryza glaberrima, Panicum italicum, Persea americana, Pisum sativum, Ricinus communis, Solanum tuberosum, Sorghum vulgare, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known higher plant polymerase II sequences, including Arabidopsis thaliana P18616, At4g35800
This antibody is not suitable for western blot in denatured conditions
Application Details:
1,2 or 5 g (IP)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
204 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Wang et al. (2020). Close arrangement of CARK3 and PMEIL affects ABA-mediated pollen sterility in Arabidopsis thaliana. Plant Cell Environ. 2020 Aug 20.doi: 10.1111/pce.13871. Godoy Herz et al. (2019). Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation. Mol Cell. 2019 Jan 15. pii: S1097-2765(18)31035-9. doi: 10.1016/j.molcel.2018.12.005.Dolata et al. (2015). NTR1 is required for transcription elongation checkpoints at alternative exons in Arabidopsis. EMBO J. 2015 Jan 7. pii: e201489478.
Special application note:
Purified IgG was lyophilized from PBS pH 7,4 + 0,02 % sodium azide, Therefore only distilled water is required for its re-constitution
CDPKs are large group of kinases found/existing? only in plants and protists. They are involved in decoding Ca2+ signals in many stress conditions. CDPKs are composed of 4 domains: N-terminal variable domain, kinase domain, autoinhibitory domain and calmodulin-resembling domain (Calcium binding domain) - EF-hand domain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Hordeum vulgare, Populus sp., Triticum aestivum
Expected Species:
Brassica napus, Nicotiana tabacum, Oryza sativa, Solanum lycopersicum, Solanum tuberosum Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Calcium-dependent protein kinase EF domain including Arabidopsis thalaiana TAIR: At4g35310 and At5g04870
RACK1A is a major component of RACK1 regulatory proteins playing a major role in multiple signal transduction pathways. Synonymes: Receptor for activated C kinase 1A, WD-40 repeat auxin-dependent protein ARCA.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Thellungiella salsuginea Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RACK1A protein sequence UniProt: O24456, TAIR: At1g18080
γ-glutamyl-cysteine is a precursor of glutathione and is synthesized by linkage of glutamate and cysteine by γ-glutamylcysteine synthetase. Synonymes:gamma-glutamyl-cysteine, γ-glutamyl-cysteine. Alternative names: γ-EC, γ-Glu-Cys.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Cucurbita pepo L. subsp. peop var. styriaca Greb, Nicotiana tabacum cv. samsun
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
γ-glutamyl-cysteine (gamma-EC) linked by glutaraldehyde
Immunogold labeling for electron microscopyBlock ultrathin sections (80nm) prepared for immunogold labeling with 2% bovine serum albumine (BSA) in phosphate buffered saline (PBS, pH 7.2). Then treat the sections with the primary antibody (anti-γ-glutamylcysteine rabbit polyclonal IgG) diluted 1:50 in PBS containing 1% BSA for 2 h at room temperature. After a short rinse in PBS (3 X 5 min), incubate samples with a gold-conjugated secondary antibody (goat anti-rabbit IgG; eg. 10nm) diluted 1:50 in PBS including 1% BSA for 90 min at room temperature. After a short wash in PBS (2 X 5 min), and distilled water (3 X 5 min) observe grids under a transmission electron microscope.
Application Details:
1 : 50 (ICC), (IG)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 100 l of sterile water per tube
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Koffler et al. (2011). Subcellular distribution of glutathione precusors in Arabidopsis thaliana. Journal of Integrative Plant Biology. 53: 930-941.
Goat anti-rabbit IgG, DyLight 350 Conjugate is a secondary antibody conjugated toDyLight 350, which binds to all rabbit IgGs in immunological assays.DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis. Purity of this antibody is > 95% based on SDS-PAGE.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Conjugate is present in 10 mM Sodium Phosphate,0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight 350 has a maximum absorbance at 353 nm; Emax = 432 nm.
Goat anti-rabbit IgG, DyLight 550 Conjugate is a secondary antibody conjugated toDyLight 550, which binds to all rabbit IgGs in immunological assays.DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.Purity of this antibody is > 95% based on SDS-PAGE.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight 550 (Ex = 550 nm; Em = 576 nm)
Goat anti-rabbit IgG, DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all rabbit IgGs in immunological assays.DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.Purity of this antibody is 95% based on SDS-PAGE.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Chong et al. (2015). Active fungal GH115 α-glucuronidase produced in Arabidopsis thaliana affects only the UX1-reactive glucuronate decorations on native glucuronoxylans. BMC Biotechnol. 2015 Jun 18;15:56. doi: 10.1186/s12896-015-0154-8.
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight 594 (Ex = 593 nm; Em = 618 nm)
Goat anti-rabbit IgG, DyLight 633 Conjugate is a secondary antibody conjugated to DyLight 633, which binds to all rabbit IgGs in immunological assays.DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis. Purity of this antibody is > 95% based on SDS-PAGE.
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight 633 (Ex = 638 nm; Em = 658 nm)
Goat anti-chicken IgY, DyLight 350 Conjugate is a secondary antibody conjugated to DyLight 350, which binds to hen immunoglobulins in immunological assays.DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis this antibody reacts with heavy chains on chicken IgG and light chains on all chicken immunoglobulins.No reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.
Application Details:
1 : 50 -1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC)
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight 350 (Ex = 353 nm; Em = 432 nm).
Goat anti-chicken IgY, DyLight 550 Conjugate is a secondary antibody conjugated to DyLight 550, which binds to hen immunoglobulins in immunological assays. DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis this antibody reacts with heavy chains on chicken IgG and light chains on all chicken immunoglobulins.No reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.
Application Details:
1 : 50 -1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC)
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight 550 (Ex = 550 nm; Em = 576 nm).
Goat anti-chicken IgY, DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to hen immunoglobulins in immunological assays. DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis this antibody reacts with heavy chains on chicken IgG and light chains on all chicken immunoglobulins.No reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.
Application Details:
1 : 50-1 : 5 000 (ICC), 1 : 20 -1 : 2000 (IHC)
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1 ml of sterile water, Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution, If necessary, centrifuge to remove any particulates, Prepare fresh working dilution daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight 594 (Ex = 593 nm; Em = 618 nm).
Goat anti-chicken IgY, DyLight 633 Conjugate is a secondary antibody conjugated to DyLight 633, which binds to hen immunoglobulins in immunological assays. DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis this antibody reacts with heavy chains on chicken IgG and light chains on all chicken immunoglobulins.No reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.
Application Details:
1 : 50 -1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC)
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight 633 (Ex = 638 nm; Em = 658 nm).
Goat anti-chicken IgY, DyLight 650 Conjugate is a secondary antibody conjugated to DyLight 650, which binds to hen immunoglobulins in immunological assays. DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis this antibody reacts with heavy chains on chicken IgY and light chains on all chicken immunoglobulins.No reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.
Application Details:
1 : 50 -1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC)
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight 650 (Ex = 652 nm; Em = 672 nm).Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)
Goat anti-chicken IgY, DyLight 800 Conjugate is a secondary antibody conjugated to DyLight 800, which binds to hen immunoglobulins in immunological assays. DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis this antibody reacts with heavy chains on chicken IgY and light chains on all chicken immunoglobulins.No reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.
Application Details:
1 : 50 -1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC)
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight 800 (Ex = 777 nm; Em = 794 nm).
Patatin is the most abundant protein in potato (Solanum tuberosum L.) tubers, about 60 % of a total protein. It serves as a storage protein. Mature tuber patatin variants are dimers of 40-42 kDa subunits without disulphide bridges.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Solanum tuberosum
Expected Species:
Solanum tuberosum
Immunogen:
KLH-conjugated synthetic peptide derived from a C-terminal part of 36 known isoforms of patatin from Solanum tuberosum including: Q3YJS9, Q3YJT0, Q42502, Q3YJT2
Applications:
Immunoprecipitation (IP), Immunolocalization (IL), Western blot (WB)
Patatin is the most abundant protein in potato (Solanum tuberosum L.) tubers, about 60 % of a total protein. It serves as a storage protein. Mature tuber patatin variants are dimers of 40-42 kDa subunits without disulphide bridges.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Solanum tuberosum
Expected Species:
Solanum tuberosum
Immunogen:
KLH-conjugated synthetic peptide derived from a C-terminal part of 36 known isoforms of patatin from Solanum tuberosum including: Q3YJS9, Q3YJT0, Q42502, Q3YJT2
Applications:
Immunoprecipitation (IP), Immunolocalization (IL), Western blot (WB)
COG0523 (Zinc defficiency induced protein) is a putative metal chaperone from G3E family of GTPases which acts in metal trafficking.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Emiliania huxleyi Species of your interest not listed? Contact us
Immunogen:
Recombinant ZCP2 protein of Chlamydomonas reinhardtii, ID 536252, UniProt: A0A059VIF5
This antibody can be used as a marker of zinc homeostasis in Chlamydomonas reinhardtii.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
70 | 100 kDa (probably due to glycosylation)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Hsieh et al. (2013). The Proteome of Copper, Iron, Zinc, and Manganese Micronutrient Deficiency in Chlamydomonas reinhardtii. Mol Cell Proteomics. 2013 Jan;12(1):65-86. doi: 10.1074/mcp.M112.021840. Epub 2012 Oct 13.
V-PPase (pyrophosphate-energized vacuolar membrane proton pump 1), EC=3.6.1.1, acts to establish proton gradient of often higher magnitude than H+-ATPase. Is involved in auxin transport and NaCl and drought tolerance. Alternative names: pyrophosphate-energized inorganic pyrophosphatase 1, H(+)-PPase 1, vacuolar proton pyrophosphatase 1, vacuolar proton pyrophosphatase 3
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Protein or membrane sample should be treated at 70 C for 10 min before loading on the gel
Application Details:
1 : 8000 (ELISA), 1 : 100 (IL), 1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
80.8 | 73 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Hofmann, Wienkoop & Luthje (2022) Hypoxia-Induced Aquaporins and Regulation of Redox Homeostasis by a Trans-Plasma Membrane Electron Transport System in Maize Roots. Antioxidants (Basel). 2022 Apr 25;11(5):836. doi: 10.3390/antiox11050836. PMID: 35624700; PMCID: PMC9137787.Prinsi et al. (2020). Root Proteomic Analysis of Two Grapevine Rootstock Genotypes Showing Different Susceptibility to Salt Stress. Int J Mol Sci. 2020 Feb 6;21(3). pii: E1076. doi: 10.3390/ijms21031076.Patir-Nebioglu et al. (2019). Pyrophosphate modulates plant stress responses via SUMOylation. Elife. 2019 Feb 20;8. pii: e44213. doi: 10.7554/eLife.44213.Migocka et al. (2015). Cucumber Metal Transport Protein CsMTP9 is a plasma membrane H+ -coupled antiporter involved in the Mn2+ and Cd2+ efflux from root cells. Plant J. 2015 Oct 20. doi: 10.1111/tpj.13056.Migocka et al. (2014). Molecular and biochemical properties of two P 1B2 -ATPases, CsHMA3 and CsHMA4, from cucumber. Plant Cell Environ. 2014 Sep 11. doi: 10.1111/pce.12447.
Special application note:
Antibodies will detect target protein in 1 g of a crude membrane preparation loaded per well, If purified preparations of vacuolar and plasma membranes are used, less than g load per well should be sufficient
UCP (uncoupling protein) is an inner membrane mitochondrial protein that can dissipate the proton gradien before it can be used to provide the energy for oxidative phosphorylation. Synonymes: AtUCP, Uncoupling protein 2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Czobor et al. (2019). Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst. PLoS One. 2019 Jan 10;14(1):e0210592. doi: 10.1371/journal.pone.0210592.Tak č et al. (2018). Shot-Gun Proteomic Analysis on Roots of Arabidopsis pldα1 Mutants Suggesting the Involvement of PLDα1 in Mitochondrial Protein Import, Vesicular Trafficking and Glucosinolate Biosynthesis. Int J Mol Sci. 2018 Dec 26;20(1). pii: E82. doi: 10.3390/ijms20010082. (immunolocalization)Garmash et al. (2017). Expression profiles of genes for mitochondrial respiratory energy-dissipating systems and antioxidant enzymes in wheat leaves during de-etiolation. J Plant Physiol. 2017 Aug;215:110-121. doi: 10.1016/j.jplph.2017.05.023.Florez-Sarasa et al. (2016). Impaired cyclic electron flow around Photosystem I disturbs high-light respiratory metabolism. Plant Physiol. 2016 Oct 19. pii: pp.01025.2016.Liu et al. (2015). Silencing of mitochondrial uncoupling protein gene aggravates chilling stress by altering mitochondrial respiration and electron transport in tomato. Acta Physiologiae Plantarum November 2015, 37:223.Long et al. (2015). Contributions of photosynthetic and non-photosynthetic cell types to leaf respiration in Vicia faba L. and their responses to growth temperature. Plant Cell Environ. 2015 Apr 1. doi: 10.1111/pce.12544.Grabelnych et al. (2014). Mitochondrial Energy Dissipating Systems (Alternative Oxidase, Uncoupling Proteins, and External NADH Dehydrogenase) Are Involved in Development of Frost Resistance of Winter Wheat Seedlings. ISSN 0006 2979, Biochemistry (Moscow), 2014, Vol. 79, No. 6, pp. 506 519. Pleiades Publishing, Ltd., 2014.Barreto et al. (2014). Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response. BMC Plant Biol. 2014 May 28;14(1):144.
Special application note:
Peptide used to elicit this antibody is conserved in both isoforms, UCP1 and UCP2 of Arabidopsis thaliana.
Halorhodopsin (HR) is a hyperpolarizing light-driven ion pump from the halophilic archaea bacterium Natronomonas pharaonis. HR uses the energy of yellow light (excitation maximum near 580 nm) to mediate primarily chloride but also bromide, iodide, and nitrate import into the cell against their electrochemical gradients.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Natronomonas pharaonis
Expected Species:
Natronomonas pharaonis Species of your interest not listed? Contact us
Immunofluorescence was performed on mouse brain slices (data not shown)
Application Details:
1 : 200 (IF), 1 : 1000-1 : 20 000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
30,9 | 30 kDa
Not reactive in:
Halobacterium salinarum (HsHR and HsBR)
Selected references:
Alfonsa et al. (2015) The contribution of raised intraneuronal chloride to epileptic network activity. J Neurosci. 2015 May 20;35(20):7715-26. doi: 10.1523/JNEUROSCI.4105-14.2015.
PSB33 or TEF5 is a Rieske (2Fe-2S) domain-containing protein located in chloroplast thylakoid membrane. The protein has oxireductase activity and is involved in oxidation reaction.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Oryza sativa
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
part of Arabidopsis thaliana recombinant TEF5 protein, corresponding to epitopes 61-242, UniProt: Q9C9I7 , TAIR: At1g71500
This product can be sold with ProClin if requested
Application Details:
1 : 4000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
31 | 25 kDa (without transit peptide)
Not reactive in:
Chlamydomonas reinhardii
Selected references:
Kato et al. (2017). Deficiency of the Stroma-Lamellar Protein LIL8/PSB33 Affects Energy Transfer Around PSI in Arabidopsis. Plant Cell Physiol. 2017 Nov 1;58(11):2026-2039. doi: 10.1093/pcp/pcx124.Fristedt et al. (2017). PSB33 sustains photosystem II D1 protein under fluctuating light conditions. Journal of Experimental Botany doi:10.1093/jxb/erx218.Dixit (2015). Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves. Sci Rep. 2015 Nov 10;5:16205. doi: 10.1038/srep16205.Fristedt at al. (2014). PSB33, a protein conserved in the plastid lineage, is associated with the chloroplast thylakoid membrane and provides stability to Photosystem II supercomplexes in Arabidopsis. Plant Physiol. Dec, 2014, open access.
CGL160 (conserved in green lineage 160) is a protein encoded in the nucleus and localized in chloroplast. It interacts with components of the CF1 sub-complex and is required for the efficient incorporation of the c-subunit into the cpATPase. Synonymes: ATCGL160.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
part of Arabidopsis thaliana recombinant CGL160 derived from a following sequence UniProt: O82279, TAIR: At2g31040
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Galvis et al. (2020). H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in chloroplast ATP synthase mutants. Plant Physiol. pp.01561.2019. doi: 10.1104/pp.19.01561.Fristedt et al. (2015). The Thylakoid Membrane Protein CGL160 Supports CF1CF0 ATP Synthase Accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.
NPR1 (regulatory protein NPR1) is a key positive regulator of the SA-dependent signaling pathway that negatively regulates JA-dependent signaling pathway. Controls the onset of systemic acquired resistance (SAR). Subcellular localization: cytoplasm, nucleaus (following induction by salicylic acid treatment or after pathogen infection. Alternative names: BTB/POZ domain-containing protein NPR1, Non-inducible immunity protein 1, Nim1, Salicylic acid insensitive 1, Sai1, ATNPR1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide, chosen from NPR1 sequence of Arabidopsis thaliana, TAIR: AT1G64280, UniProt: P93002
Please note that depending upon detection system you are using, longer exposure time may be required with this antibody, Chemiluminescent detection reagent in extreme femtogram range is necessary
Arenas-Alfonseca et al. (2021) Arabidopsis beta-cyanoalanine synthase mutation overcomes NADPH oxidase action in response to pathogens. J Exp Bot. 2021 Mar 26:erab137. doi: 10.1093/jxb/erab137. Epub ahead of print. PMID: 33770168.Nomoto et al. (2021) Suppression of MYC transcription activators by the immune cofactor NPR1 fine-tunes plant immune responses. Cell Rep. 2021 Dec 14;37(11):110125. doi: 10.1016/j.celrep.2021.110125. PMID: 34910911.Lei et al. (2020). Construction of gold-siRNANPR1 nanoparticles for effective and quick silencing of NPR1 in Arabidopsis thaliana. DOI: 10.1039/D0RA02156C (Paper) RSC Adv., 2020, 10, 19300-19308
Special application note:
For successful detection using NPR1 antibody please follow protocol suggested below. NPR1 protein readily oligomerizes, in addition to any naturally occurring oligomer, during extraction. Therefore 50 mM DTT has to be used as well as denaturation at 75 C for 15 minutes. Engogenous NPR1 level is very low, thereofre SA treatment is absolutely necessary for good detection.This antibody is recognizing NPR1-GFP in the 35S overexpression line.
NPR1 (regulatory protein NPR1) is a key positive regulator of the SA-dependent signaling pathway that negatively regulates JA-dependent signaling pathway. Controls the onset of systemic acquired resistance (SAR). Subcellular localization: cytoplasm, nucleaus (following induction by salicylic acid treatment or after pathogen infection. Alternative names: BTB/POZ domain-containing protein NPR1, Non-inducible immunity protein 1, Nim1, Salicylic acid insensitive 1, Sai1, ATNPR1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide, chosen from NPR1 sequence of Arabidopsis thaliana, TAIR: AT1G64280, UniProt: P93002
For successful detection using NPR1 antibody please follow protocol suggested below. NPR1 protein readily oligomerizes, in addition to any naturally occurring oligomer, during extraction. Therefore 50 mM DTT has to be used as well as denaturation at 75 C for 15 minutes. Engogenous NPR1 level is very low, thereofre SA treatment is absolutely necessary for good detection.This antibody is recognizing NPR1-GFP in the 35S overexpression line.
Ribosomal S6 kinase 1/2 (S6K1/2) involved in TOR signaling pathway, in osmotic stress response. Activated by PDK1 and repressed during osmotic stress. Expressed in all tissues, especially during high metabolic activity in growing buds, root tips, leaf margins and germinating seeds. Alternative names: AtPK1/AtPK6 S6K1), AtPK2/AtPK19 (S6K2).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Physcomitrella patens, Thelungiella halophilaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide, derived from Arabidopsis thaliana S6K1: UniProt: P42818, TAIR: AT3G08730 and S6K2: UniProt: Q39030, TAIR: AT3G08720. Due to high amino acid homology, chosen peptide is conserved in both proteins: S6K1 and S6K2.
Keeping the samples at 4 C all times is of crucial importance, Laucs buffer was used in example below since this is the one which was used routinely by this test laboratory for the work with kinases, Some unspecific bands can be seen, depending upon western blot protocol which is used, Therefore please consider to use a negative control together with your samples
Gonz lez-L pez et al. (2021). Growth promotion in Arabidopsis thaliana by bacterial cyclodipeptides involves the TOR/S6K pathway activation. Journal of Plant Physiology. Volume 257, 2021, 153343,ISSN 0176-1617, https://doi.org/10.1016/j.jplph.2020.153343.Salazar-Diaz et al. (2021) TOR senses and regulates spermidine metabolism during seedling establishment and growth in maize and Arabidopsis. iScience. 2021 Oct 12;24(11):103260. doi: 10.1016/j.isci.2021.103260. PMID: 34765910; PMCID: PMC8571727.Angelos & Brandizzi (2021). The UPR regulator IRE1 promotes balanced organ development by restricting TOR-dependent control of cellular differentiation in Arabidopsis. Plant J. 2021 Dec 11. doi: 10.1111/tpj.15629. Epub ahead of print. PMID: 34902186.Kazibwe et al. (2020). TOR mediates the autophagy response to altered nucleotide homeostasis in a ribonuclease mutant. J Exp Bot. 2020 Sep 9;eraa410.doi: 10.1093/jxb/eraa410.Dong et al. (2019). The Arabidopsis THADA homologue modulates TOR activity and cold acclimation. Plant Biol (Stuttg). 2019 Jan;21 Suppl 1:77-83. doi: 10.1111/plb.12893.
Special application note:
So far in applied conditions and extracts endogenous S6K1/2 protein is detectable as a very weak band
SOC1 (Suppressor of constans overexpression 1) is a transcription activator active in flowering time control. Located in nucleus and cytoplasm. Widely expressed but not found in the apical meristem of short-day grown plants in vegetative stage. Alternative names: AGAMOUS-LIKE 25, AGL25, FLC, FLF, FLOWERING LOCUS C, FLOWERING LOCUS F.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica sp., Cardamine sylvatica, Sinapsis juncea Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptidederived from Arabidpsis thaliana SOC1. UniProt: O64645,TAIR: AT2G45660
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Cuerda-Gil et al. (2022) A plant tethering system for the functional study of protein-RNA interactions in vivo. Plant Methods. 2022 Jun 4;18(1):75. doi: 10.1186/s13007-022-00907-w. PMID: 35658900; PMCID: PMC9166424.
FLS2 (Flagellin-sensitive 2) is a pattern-recognition receptor and a single-pass membrane protein. Alternative names: FLAGELLIN-SENSITIVE 2, FLAGELLIN-SENSING 2, FLS2, LRR receptor-like serine/threonine-protein kinase FLS2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Capsella rubella, Glycine max, Hordeum vulgare, Populus trichocarpa, Solanum lycopersivum, Vitis viniferaSpecies of your interest not listed? Contact us
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Kalischuk et al. (2022) Amplification of cell signaling and disease resistance by an immunity receptor Ve1Ve2 heterocomplex in plants. Commun Biol. 2022 May 25;5(1):497. doi: 10.1038/s42003-022-03439-0. PMID: 35614138; PMCID: PMC9132969.Wang et al. (2021) Arabidopsis PUB2 and PUB4 connect signaling components of pattern-triggered immunity. New Phytol. 2021 Dec 17. doi: 10.1111/nph.17922. Epub ahead of print. PMID: 34918346.Ngou et al. (2021) Mutual potentiation of plant immunity by cell-surface and intracellular receptors. Nature. 2021 Mar 10. doi: 10.1038/s41586-021-03315-7. Epub ahead of print. PMID: 33692545.Hilleary et al. (2020). Tonoplast-localized Ca2+ pumps regulate Ca2+ signals during pattern-triggered immunity in Arabidopsis thaliana. Proc Natl Acad Sci U S A . 2020 Aug 4;117(31):18849-18857.doi: 10.1073/pnas.2004183117. Zhang et al. (2019). An important role of L -fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity. New Phytol. 2018 Dec 15. doi: 10.1111/nph.15639.
BAK1 (Brassinosteroid insensitive 1-associated receptor kinase 1) controls expression of genes associated with innate immunity in the absence of pathogens or elicitors. Phosphorylated Brl1. Involved in programmed cell death (PCD). Subcellular localization: cell membrane. Alternative names: BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1, BAK1, BRI1-ASSOCIATED RECEPTOR KINASE, RKS10, RECEPTOR KINASES LIKE SERK 10, SERK3, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3, ELG, ELONGATED, ATSERK3, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3, ATBAK1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Solanum lycopersicum
Expected Species:
Thelungiella halophila Species of your interest not listed? Contact us
Extra Information on CE extraction buffer: CE buffer does not need to be made freshly everytime. Aliquots can be kept at -20 °C. Na2MoO4 and NaF are phosphatase inhibitors, included to prevent lose phosphorylation form our protein of interest during extraction. EDTA chelates metal ions and thus inhibits many enzymes which need metal ions as co-factors and inhibits the action of proteases. Protease inhibitor coctail is Sigma product number P 9599 which is used in dilution 1: 100.Antibody is reconizing AtBAK1-Myc in total extracts.
Xu et al. (2022) The Phloem Intercalated With Xylem-Correlated 3 Receptor-Like Kinase Constitutively Interacts With Brassinosteroid Insensitive 1-Associated Receptor Kinase 1 and Is Involved in Vascular Development in Arabidopsis. Front Plant Sci. 2022 Jan 11;12:706633. doi: 10.3389/fpls.2021.706633. PMID: 35087541; PMCID: PMC8786740.Kalischuk et al. (2022) Amplification of cell signaling and disease resistance by an immunity receptor Ve1Ve2 heterocomplex in plants. Commun Biol. 2022 May 25;5(1):497. doi: 10.1038/s42003-022-03439-0. PMID: 35614138; PMCID: PMC9132969.Katarzyna Parys et al (2021) Signatures of antagonistic pleiotropy in a bacterial flagellin epitope,Cell Host & Microbe,Volume 29, Issue 4,2021,Pages 620-634.e9,ISSN 1931-3128,https://doi.org/10.1016/j.chom.2021.02.008.(https://www.sciencedirect.com/science/article/pii/S1931312821000871)Zhang et al. (2019). An important role of L -fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity. New Phytol. 2018 Dec 15. doi: 10.1111/nph.15639.Hu et al. (2018). A group of receptor kinases are essential for CLAVATA signalling to maintain stem cell homeostasis. Nat Plants. 2018 Apr;4(4):205-211. doi: 10.1038/s41477-018-0123-z. (CoIP)
Special application note:
Antibodies bind to AtBAK1-Myc. They do not cross react with AtSOBIR.This product can be sold containing proclin if requested.
BRI1 (Protein BRASSINOSTEROID INSENSITIVE 1) is a receptor which binds brassinolide and has a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates. Involved in a signaling cascade including expression of light- and stress-regulated genes, promotion of cell elongation, normal leaf and chloroplast senescence, and flowering. Alternative names:BRI1, BRASSINOSTEROID INSENSITIVE 1, CBB2, CABBAGE 2, DWF2, DWARF 2, BIN1, BR INSENSITIVE 1, ATBRI1, Brassinosteroid LRR receptor kinase
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus, Brassica rapaSpecies of your interest not listed? Contact us
Antibody was tested on bri1-1 and bri1-5 mutants. Bri1-1 is a point mutation in the kinase domain that renders the protein non-functional and plants compensate for that by over-accumulating the non-functional receptor. Bri1-5 is a mutant in the extracellular domain and the bri1-5 protein is retained in the ER. The bri1-5 plants contain less protein than the wild type and show an intermediate brassinosteroid deficient phenotype. Also BRI1-5 migrates higher than wild type BRI1 in SDS-PAGE, because it carries ER-type high mannose N-glycans. For IP: 15 l GFP-trap beads was used for 200 mg plant material to precipitate GFP-tagged protein followed by detection with Co-IPed BRI1 on Western with 1:5000 diluted anti-BRI1 antibody.Protein extraction has to be done efficiently as this step is crucial, recommended material to buffer ratio: 15 l/ g or less.
Lee et al (2021). Chaperone-like protein DAY plays critical roles in photomorphogenesis. Nat Commun. 2021 Jul 7;12(1):4194. doi: 10.1038/s41467-021-24446-5. PMID: 34234144; PMCID: PMC8263706.Chen et al. (2019). BES1 is activated by EMS1-TPD1-SERK1/2-mediated signaling to control tapetum development in Arabidopsis thaliana. Nat Commun. 2019 Sep 13;10(1):4164. doi: 10.1038/s41467-019-12118-4.Hou et al. (2019). Less Conserved LRRs Is Important for BRI1 Folding. Front Plant Sci. 2019 May 21;10:634. doi: 10.3389/fpls.2019.00634. eCollection 2019.Chen et al. (2019). BZR1 Family Transcription Factors Function Redundantly and Indispensably in BR Signaling but Exhibit BRI1-Independent Function in Regulating Anther Development in Arabidopsis. Mol Plant. 2019 Jun 20. pii: S1674-2052(19)30207-2. doi: 10.1016/j.molp.2019.06.006.
Special application note:
This product can be sold containing proclin if requested
ABI1 is a key component and repressor of the abscisic acid (ABA) signaling pathway. It regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane, drought-induced resistance and rhizogenesis, response to glucose, high light stress, seed germination and inhibition of vegetative growth. Expressed in seeds and seedlings. Confined to lateral root caps and columella cells in roots. Induced by low temperature, drought, high salt, ABA and ethylene. Activates/represses SnRK2.6/SRK2E/OST1 in response to ABA-dependent stimuli. Alternative names: ABI1, ABA INSENSITIVE 1, AtABI1, Protein phosphatase 2C 56, AtPP2C56, PP2C56.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide, derived from Arabidopsis thaliana ABI1 sequence UniProt: P49597, TAIR: AT4G26080. Chosen peptide is not present in AtABI2.
Important note: blocking with more than 3 % skimmed milk will result in lack of signal for this antibody
Application Details:
5 g (IP for a 200 ul of a cell extract), 3 g (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
47,5 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Mitula et al. (2015). Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway. Plant Cell Physiol. 2015 Dec;56(12):2351-67. doi: 10.1093/pcp/pcv146. Epub 2015 Oct 6.
Special application note:
It is of crucial importance to chose a material in which ABI1 protein is highly expressed like seeds or senescent leaf. This protein could not be detected using this antibody in plants grown under optimal (non stressed) conditions, The antibody detects both, recombinant and endogenous ABI1 proteins.
ABI5 (abscisic acid insensitive 5) is involved in ABA-regulated gene expression during seed development and subsequent vegetative stage and acts as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Alternative names: ABI5, ABA INSENSITIVE 5, GIA1, GROWTH-INSENSITIVITY TO ABA 1, Dc3 promoter-binding factor 1, AtDPBF1, GROWTH-INSENSITIVITY TO ABA 1, bZIP transcription factor 39, AtbZIP39.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus, Populus trichocarpaSpecies of your interest not listed? Contact us
ABI5 protein is present in very low levels therefore specific material should be used for analysis as well as chemiluminescence detection reagent in extreme low femtogram range, as AgriseraECLSuperBright.
Application Details:
1: 140 (IL), 1 : 200 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water to each tube
Molecular Weight:
47 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Special application note:
MG132 is recommened to be added to extraction buffer as ABI5 is degraded by proteasome as well as homogenization with thiourea and bead beater. Protocol for protein extraction from seeds can be requested here.
GI (GIGANTEA) is involved in several developmental processes including regulation of circadian rhythm and photoperiodic flowering, phytochrome B signaling, circadian clock, carbohydrate metabolism and cold stress response.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica campestris, Chrysanthemum morifolium, Dimocarpus longan, Festuca patensis, Gentiana triflora, Glycine soja, Hordeum vulgare, Liriodendron tulipifera, Lolium perenne, Lotus japonicus, Medicago truncatula, Plantago major, Populus balsamifera, Prunus dulcius, Ricinus communis, Secale cereale, Theobroma cacao, Triticum aestivumSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana GI, UniProt:Q9SQI2,TAIR: AT1G22770
EIN2 (Ethylene-insensitive protein 2) is a central factor in signaling pathways regulated by ethylene including various processes like: plant defense and development, senescence, nucleotide sugar flux and tropisms. Alternative names: EIN2, ETHYLENE INSENSITIVE 2, PIR2, CKR1, CYTOKININ RESISTANT 1, ERA3, ENHANCED RESPONSE TO ABA3, ORE3, ORESARA 3, ORE2, ORESARA 2, ATEIN2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brachypodium distachyon, Cucumis sativus, Glycine max, Hordeum vulgare, Medicago truncatula, Nicotiana tabacum, Solanum lycopersicum, Oryza sativa, Phtheirospermum japonicum, Populus trichocarpa, Ricinus communis, Solanum lycopresicum, Sorghum bicolor, Triticum aestivum, , Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide chosen from EIN2 of Arabidopsis thaliana, UniProt: Q9S814, TAIR: AT5G03280Chosen peptide is not to be found in EIN3.
HY5 (Protein long hypocotyl 5) is a light-regulated transcription factor that promotes photomorphogenesis in light. Involved in the blue light specific pathway. Heterodimer. Expressed in root, hypocotyl, cotyledon, leaf, stem and floral organs. Localized in nucleus. Alternative names: Protein LONG HYPOCOTYL 5, bZIP transcription factor 56, AtbZIP56.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica pekinensisSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide, derived from Arabidopsis thaliana HY5 protein sequence, UniProt:O24646 , TAIR: AT5G11260. Chosen peptide is not conserved in HY5 protein sequence.
This antibody detects recombinant HY5 in Nicotiana benthamiana.Extraction and loading buffer with 6-8 M urea buffer needs to be used when working with endogenous extract to allow detection with this antibody or TCA/acetone protein extraction or as described in Mechin et al. (2007).
Cazzonelli et al. (2019). A cis-carotene derived apocarotenoid regulates etioplast and chloroplast development. https://doi.org/10.1101/528331Lee et al. (2017). The F-box protein FKF1 inhibits dimerization of COP1 in the control of photoperiodic flowering. Nat Commun. 2017 Dec 22;8(1):2259. doi: 10.1038/s41467-017-02476-2.Sinclair et al. (2017) Etiolated Seedling Development Requires Repression of Photomorphogenesis by a Small Cell-Wall-Derived Dark Signal. Curr Biol. 2017 Nov 20;27(22):3403-3418.e7. doi: 10.1016/j.cub.2017.09.063.
MYC2 (Transcription factor MYC2) acts as a transcriptional activator for light, abscisic acid (ABA) and jasmonic acid (JA) signaling pathways. It is involved in the regulation of ABA-inducible genes under drought stress conditions.Alternative names: ATMYC2, JAI1, JASMONATE INSENSITIVE 1, JIN1, Basic helix-loop-helix protein 6, AtbHLH6, bHLH transcription factor bHLH006, R-homologous Arabidopsis protein 1, RAP-1, Transcription factor EN 38, ZBF1, Z-box binding factor 1 protein, RD22BP1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica oleraceaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide, derived from MYC2 sequence of Arabidopsis thaliana UniProt: Q39204, TAIR: AT1G32640. This peptide is not conserved in MYC1, MYC3 and MYC4.
Load per well: 0.5 -1 g of purified, recombinant MYC2 protein or 10-20 g for non-treated material from plants overexpressing MYC2 or 1-5 g for a treated material from plants overexpressing MYC2. Nuclear extract is recommended for endogenous samples.
Protein Abscisic acid-insensitive 2 (ABI2) is a represor of the ABA signaling pathway, regulating various ABA responses like: stomatal closure, osmotic water permability of the plasma membrane, high light stress, response to glucose, seed germination and inhibition of vegetative growth. Involved in acquired thermotolerance. Alternative names: ABA INSENSITIVE 2, ABI2, ATABI2, Protein phosphatase 2C 77, AtPP2C77, At5g57050, MHM17_19, MHM17.19, PP2C ABI2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from N-terminus of Arabidopsis thaliana ABI2 sequence, UniProt: O04719, ,TAIR: AT5G57050 chosen peptide is not conserved in ABI1
ABI2 antibodies recognize recombiant StrepTag-ABI2, ABI2-GST, His-ABI2. ABI2 protein is easily degraded therefore extraction buffer needs to contain protease inhibotors, example of such inhibitor coctail can be found here.To detect endogenous ABI2 plant material needs to be subjected to stress before harvesting.
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 l of sterile water
Molecular Weight:
46 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Mitula et al. (2015). Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway. Plant Cell Physiol. 2015 Dec;56(12):2351-67. doi: 10.1093/pcp/pcv146. Epub 2015 Oct 6.
NtcA acts as a transcriptional activator of genes subjected to nitrogen control.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Synechocystis sp. PCC6803
Expected Species:
Anabaena sp., Gleobacter sp., marine Synechococcus strains, Trichodesmium sp. Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known cyanobacterial NtcA protein sequences including UniProt: P33779
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Ge at al. (2017). Translating Divergent Environmental Stresses into a Common Proteome Response through the Histidine Kinase 33 (Hik33) in a Model Cyanobacterium. Mol Cell Proteomics. 2017 Jul;16(7):1258-1274. doi: 10.1074/mcp.M116.068080.
DGAT2A (Acyl-CoA: Diacylglycerol acyltransferase) is an enzyme which exhibits transferase activity, transferring acyl groups. EC=2.7.8.2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Chlamydomonas reinhardtii
Immunogen:
recombinant CrDGAT2A, overexpressed in E.coli, missing transmembrane domains, PID 536226, also annotated as DGTT1 UniProt: A8JGY1
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Liu et al. (2016). A simple and reproducible non-radiolabeled in vitro assay for recombinant acyltransferases involved in triacylglycerol biosynthesis. J Appl Phycol (2016). doi:10.1007/s10811-016-0949-6.Wase et al. (2015). Phenotypic screening identifies Brefeldin A/Ascotoxin as an inducer of lipid storage in the algae Chlamydomonas reinhardtii. Algal Research, Volume 11, September 2015, Pages 74–84.
PDAT1 is an enzyme phospholipid: diacylglycerol acyltransferase, which catalyzes TAG (triglycerols) synthesis. It has a strong lipase activity with a broad substrate specificity.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Chlamydomonas reinhardtii
Immunogen:
recombinant CrPDAT1 without transmembrane domains, overexpressed in E.coli,
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Yoon et al (2012). Phospholipid:Diacylglycerol Acyltransferase Is a Multifunctional Enzyme Involved in Membrane Lipid Turnover and Degradation While Synthesizing Triacylglycerol in the Unicellular Green Microalga Chlamydomonas reinhardtii. Plant Cell, Oct 2012.
Donkey anti-Goat IgG (H&L) - DyLight 550 Conjugated is a secondary antibody conjugated to DyLight 550, which binds to all goat IgGs in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase goat IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy chains on goat IgG light chains on all goat immunoglobulins.No reactivity is observed to: non-immunoglobulin goat serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Goat IgG (H&L) - DyLight 550 Conjugated, min cross reactivity to human, mouse, rabbit or rat IgG is a secondary antibody conjugated to DyLight 550, which binds to all goat IgGs in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase goat IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on goat IgG light chains on all goat immunoglobulins.No reactivity is observed to: non-immunoglobulin goat serum proteins IgG/serum from human, mouse, rabbit or rat.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Rabbit anti-goat IgG (H&L) - DyLight 550 Conjugated, min cross reactivity to human, mouse, rat lgG is a secondary antibody conjugated to DyLight 550, which binds to all goat IgGs in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase goat IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on goat IgG light chains on all goat immunoglobulin.No reactivity is observed to: non-immunoglobulin goat serum proteins IgG from human, mouse, or rat.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-guinea pig IgG (H&L) - DyLight 550 Conjugate is a secondary antibody conjugated to DyLight 550, which binds to all guinea pig IgG in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase guinea pig IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on guinea pig IgG light chains on all guinea pig immunoglobulins.No reactivity is observed to: non-immunoglobulin guinea pig serum proteins
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-human IgA (α chain) - DyLight 550 Conjugate, min cross reactivity to bovine, mouse, rabbit serum is a secondary antibody conjugated to DyLight 550, which binds to all human IgA (α chain) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase human IgA.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (α) chains on human IgA (α chain)No reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins serum proteins from bovine, mouse or rabbit.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-human IgG (H&L) - DyLight 550 Conjugated is a secondary antibody conjugated to DyLight 550, which binds to all human IgG in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase human IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on human IgG ( chain) light chains on all human immunoglobulins.No reactivity is observed to: non-immunoglobulin human serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-human IgG Fc - DyLight 550 Conjugated is a secondary antibody conjugated to DyLight 550, which binds to all human IgG, Fc in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase human IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on human IgG.No reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-human IgM ( chain) - DyLight 550 Conjugated, min. cross-reactivity to human IgA + IgG is a secondary antibody conjugated to DyLight 550, which binds to all human IgM ( chain) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase human IgM.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy ( ) chains on human IgM.No reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins human IgA + IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Rabbit anti-llama IgG (H&L) - DyLight 550 Conjugate is a secondary antibody conjugated to DyLight 550, which binds to all llama IgG in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase llama IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on llama IgG light chains on all llama immunoglobulins.This antibody will react with VHH of llama IgG's.No reactivity is observed to: non-immunoglobulin llama serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications (ELISA, Flow cytometry, Immunofluorescence, Immunolocalization)
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Chicken anti-Mouse IgG (H&L) - DyLight 550 Conjugate is a secondary antibody conjugated to DyLight 550, which binds to all mouse IgG in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase mouse IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.,Chicken anti-Mouse IgG (H&L) - DyLight 550 Conjugate is a secondary antibody conjugated to DyLight 550, which binds to all mouse IgG in immunological assays.DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase mouse IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications,1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified chicken IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Chicken anti-Mouse IgG (H&L) - DyLight 550 Conjugated, min. cross reactivity to human or rabbit IgG and serum proteins is a secondary antibody conjugated to DyLight 550, which binds to all mouse IgG in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase mouse IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep.
Application Details:
1 : 20-1 : 2000 for most applications,1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified chicken IgG.
Reconstitution:
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Mouse IgG (H&L) - DyLight 550 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG is a secondary antibody conjugated to DyLight 550, which binds to all mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase mouse IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8 C, Product is stable for 4 weeks at 2-8°Cafter rehydration,For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity, For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol, Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol, Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-mouse IgG (H&L) - DyLight 550 Conjugated is a secondary antibody conjugated to DyLight 550, which binds to all mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase mouse IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-mouse IgG (H&L) - DyLight 550 Conjugated, min. cross-reactivity to human IgG or serum proteins is a secondary antibody conjugated to DyLight 550, which binds to all mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase mouse IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins human IgG or serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-mouse IgG (H&L) - DyLight 550 Conjugated, min. cross-reactivity to bovine, goat, human, rabbit, rat IgG is a secondary antibody conjugated to DyLight 550, which binds to all mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase mouse IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins IgG from bovine, goat, human, rabbit or rat.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-mouse IgG (H&L) - DyLight 550 Conjugated, min. cross-reactivity to bovine, goat, human, rabbit, rat IgG (highly absorbed against rat IgG) is a secondary antibody conjugated to DyLight 550, which binds to all mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase mouse IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins IgG from bovine, goat, human, rabbit or rat.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-mouse IgG (H&L) - F(ab)'2 fragment, DyLight 550 Conjugate, min. cross-reactivity to bovine, horse, human, pig or rabbit serum proteins is a secondary antibody conjugated to DyLight 550, which binds to all mouse IgG (H&L), F(ab)'2 fragment in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase mouse IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins serum proteins from bovine, horse, human, pig, or rabbit.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Rabbit anti-mouse IgG (H&L) - DyLight 550 Conjugate, min. cross-reactivity to human serum is a secondary antibody conjugated to DyLight 550, which binds to all mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase mouse IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins human serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Chicken anti-Rabbit IgG (H&L) - Affinity Pure, DyLight 550 Conjugate is a secondary antibody conjugated to DyLight 550, which binds to all rabbit IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified chicken IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rabbit IgG (H&L) - Affinity Pure, DyLight 550 Conjugate is a secondary antibody conjugated to DyLight 550, which binds to all rabbit IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rabbit IgG (H&L) - DyLight 550 Conjugated, min. cross-reactivity to human IgG and serum proteins is a secondary antibody conjugated to DyLight 550, which binds to all rabbit IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins mouse IgG or serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rabbit IgG (H&L) - DyLight 550 Conjugate, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG is a secondary antibody conjugated to DyLight 550, which binds to all rabbit IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-rabbit IgG (H&L) - DyLight 550 Conjugate, min. cross-reactivity to bovine, human, mouse IgG or serum proteins is a secondary antibody conjugated to DyLight 550, which binds to all rabbit IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from human or mouse serum proteins from bovine, human or mouse.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-rabbit IgG (H&L) - F(ab)'2 fragment, DyLight 550 Conjugate, min. cross-reactivity to bovine, human, or mouse lgG or serum proteins is a secondary antibody conjugated to DyLight 550, which binds to all rabbit IgG (H&L), F(ab)'2 fragment in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from bovine, human or mouse serum proteins from bovine, human or mouse.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Provost et al. (2019). Sci Rep. 2019 Nov 29;9(1):17967. doi: 10.1038/s41598-019-53528-0.
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-rabbit IgG Fc - Affinity Pure, DyLight 550 Conjugate is a secondary antibody conjugated to DyLight 550, which binds to all rabbit IgG (H&L), Fc in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG.No reactivity is observed to: non-immunoglobulin rabbit serum proteins light chains on all rabbit immunoglobulins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Chicken anti-Rat IgG (H&L) - DyLight 550 Conjugate, min. cross-reactivity to human or rabbit IgG or serum proteins is a secondary antibody conjugated to DyLight 550, which binds to all rat IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rat IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG light chains on all rat immunoglobulins.No reactivity is observed to: non-immunoglobulin rat serum proteins IgG from human or rabbit serum proteins from human or rabbit.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified chicken IgG.
Reconstitution:
For reconstitution add 0.55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rat IgG (H&L) - Affinity Pure, DyLight 550 Conjugate is a secondary antibody conjugated to DyLight 550, which binds to all rat IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rat IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG light chains on all rat immunoglobulins.No reactivity is observed to: non-immunoglobulin rat serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rat IgG (H&L) - DyLight 550 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG is a secondary antibody conjugated to DyLight 550, which binds to all rat IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rat IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG light chains on all rat immunoglobulins.No reactivity is observed to: non-immunoglobulin rat serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit or sheep.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-Rat IgG (H&L) - Affinity Pure, DyLight 550 Conjugate is a secondary antibody conjugated to DyLight 550, which binds to all rat IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rat IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG light chains on all rat immunoglobulins.No reactivity is observed to: non-immunoglobulin rat serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-Rat IgG (H&L), F(ab)'2 fragment - DyLight 550 Conjugated, min. cross-reactivity to human, mouse lgG is a secondary antibody conjugated to DyLight 550, which binds to all rat IgG (H&L), F(ab) fragment in immunological assays.DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rat IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG light chains on all rat immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins IgG from human or mouse.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Sheep IgG (H&L) - DyLight 550 Conjugated, min. cross reactivity to human or rabbit IgG is a secondary antibody conjugated to DyLight 550, which binds to all Sheep IgG (H&L) in immunological assays. DyLight 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase Sheep IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG light chains on all sheep immunoglobulins.No reactivity is observed to: non-immunoglobulin sheep serum proteins IgG from human or rabbit.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Goat IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all goat IgGs in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase goat IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy chains on goat IgG light chains on all goat immunoglobulins.No reactivity is observed to: non-immunoglobulin goat serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Selected references:
Ainla et al. (2013). Lab on a Biomembrane: rapid prototyping and manipulation of 2D fluidic lipid bilayers circuits. Sci Rep. 2013 Sep 25;3:2743. doi: 10.1038/srep02743.
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Goat IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to human, mouse, rabbit or rat IgG is a secondary antibody conjugated to DyLight 594, which binds to all goat IgGs in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase goat IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on goat IgG light chains on all goat immunoglobulins.No reactivity is observed to: non-immunoglobulin goat serum proteins IgG/serum from human, mouse, rabbit or rat.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Rabbit anti-goat IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all goat IgGs in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase goat IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on goat IgG light chains on all goat immunoglobulins.No reactivity is observed to: non-immunoglobulin goat serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-guinea pig IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all guinea pig IgG in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase guinea pig IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on guinea pig IgG light chains on all guinea pig immunoglobulins.No reactivity is observed to: non-immunoglobulin guinea pig serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-guinea pig IgG (H&L) , DyLight 594 Conjugated, min. cross reactivity to bovine, chicken, goat, hamster, horse, human, mouse, rabbit, rat, sheep Serum is a secondary antibody conjugated to DyLight 594, which binds to all guinea pig IgG in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase guinea pig IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on guinea pig IgG light chains on all guinea pig immunoglobulins.No reactivity is observed to: non-immunoglobulin guinea pig serum proteins serum proteins from bovine, chicken, goat, hamster, horse, human, mouse, rabbit, rat or sheep.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-human IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all human IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase human IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on human IgG (γchain) light chains on all human immunoglobulins.No reactivity is observed to: non-immunoglobulin human serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-human IgG Fc , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all human IgG Fc in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase human IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on human IgG.No reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-human IgM ( chain) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all human IgM ( chain) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase human IgM.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy ( ) chains on human IgM.No reactivity is observed to: non-immunoglobulin human serum proteins light chains on all human immunoglobulins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rabbit IgG (H&L) - Affinity Pure, DyLight 633 Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG is a secondary antibody conjugated to DyLight 633, which binds to rabbit IgG (H&L) in immunological assays.DyLight 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
immunolocalization Donkey anti-rabbit conjugated to DY Light 633 in the absence of primary antibody show very little background label (right image) and binds to primary antibodies (anti-Arf1 and anti-Sec21p; two left images). The top images show only the antibody label in red and bottom images show overlay of antibody label in red with DAPI in blue and DIC/Nomarski image in grey. The material has been prepared using whole mount immunolabelling procedure of 5 day old Arabidopsis thaliana seedlings (Fischer, U. et al. (2006). Curr. Biol. 16, 2143–2149). Antibody dilutions: rabbit anti-sec21p (Agrisera, AS08 327) 1:1000, rabbit anti-Arf1 (Agrisera, AS08 325) 1:1000 and Agrisera Donkey anti-rabbit DY Light 633 1:100. DNA was stained with DAPI. Courtesy: Dr. Anna Gustavsson and Prof. Markus Grebe, Ume Plant Science Centre
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, no reactivity is observed to:non-immunoglobulin rabbit serum proteinsIgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat.Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.
Goat anti-human kappa light chain , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all human kappa light chain in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase human kappa light chain.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: kappa light chains on human immunoglobulins.No reactivity is observed to: non-immunoglobulin human serum proteins heavy chains on human immunoglobulins lambda light chains on human immunoglobulins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Rabbit anti-human IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all human IgG in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase human IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on human IgG light chains on all human immunoglobulins.No reactivity is observed to: non-immunoglobulin human serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Rabbit anti-llama IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all llama IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase llama IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on llama IgG light chains on all llama immunoglobulins.This antibody will react with VHH of llama IgG's.No reactivity is observed to: non-immunoglobulin llama serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified rabbit IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Mouse IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase mouse IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Mouse IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG is a secondary antibody conjugated to DyLight 594, which binds to all mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase mouse IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-mouse IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to al mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase mouse IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-mouse IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to human IgG or serum proteins is a secondary antibody conjugated to DyLight 594, which binds to al mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase mouse IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins human IgG or serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-mouse IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to bovine, horse, human, pig or rabbit serum proteins is a secondary antibody conjugated to DyLight 594, which binds to all mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase mouse IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins serum proteins from bovine, horse, human, pig, or rabbit.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-mouse IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to bovine, goat, human, rabbit, rat IgG (highly absorbed against rat IgG) is a secondary antibody conjugated to DyLight 594, which binds to all mouse IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase mouse IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG light chains on all mouse immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins IgG from bovine, goat, human, rabbit or rat.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Chicken anti-Rabbit IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all rabbit IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified chicken IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rabbit IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all rabbit IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rabbit IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to human IgG and serum proteins is a secondary antibody conjugated to DyLight 594, which binds to all rabbit IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins mouse IgG or serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rabbit IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG is a secondary antibody conjugated to DyLight 594, which binds to all rabbit IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat or sheep.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-rabbit IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to bovine, human, mouse IgG or serum proteins is a secondary antibody conjugated to DyLight 594, which binds to all rabbit IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from human or mouse serum proteins from bovine, human or mouse.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-rabbit IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to bovine, goat, human, mouse, rat IgG or serum proteins is a secondary antibody conjugated to DyLight 594, which binds to all rabbit IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins serum proteins from bovine, goat, human, mouse or rat IgG from bovine, goat, human, mouse or rat.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-rabbit IgG (H&L) - F(ab)'2 fragment, DyLight 594 Conjugated, min. cross-reactivity to bovine, human, or mouse lgG or serum proteins is a secondary antibody conjugated to DyLight 594, which binds to all rabbit IgG (H&L), F(ab)'2 fragment in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.No reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from bovine, human or mouse serum proteins from bovine, human or mouse.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-rabbit IgG Fc , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all rabbit IgG Fc in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rabbit IgG Fc.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rabbit IgG.No reactivity is observed to: non-immunoglobulin rabbit serum proteins light chains on all rabbit immunoglobulins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Rat IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rabbit or sheep IgG is a secondary antibody conjugated to DyLight 594, which binds to rat IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rat IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG light chains on all rat immunoglobulins.No reactivity is observed to: non-immunoglobulin rat serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit or sheep.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Goat anti-Rat IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all rat IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rat IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG light chains on all rat immunoglobulins.No reactivity is observed to: non-immunoglobulin rat serum proteins.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified goat IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Sheep IgG (H&L) , DyLight 594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all sheep IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase sheep IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG light chains on all sheep immunoglobulins.No reactivity is observed to: non-immunoglobulin sheep serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Sheep IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to human or rabbit IgG is a secondary antibody conjugated to DyLight 594, which binds to all sheep IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase sheep IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG light chains on all sheep immunoglobulins.No reactivity is observed to: non-immunoglobulin sheep serum proteins IgG from human or rabbit.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
Donkey anti-Sheep IgG (H&L) , DyLight 594 Conjugated, min. cross-reactivity to human,mouse, or rabbit IgG is a secondary antibody conjugated to DyLight 594, which binds to all sheep IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase sheep IgG.DyLight is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°Cafter rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20 °C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20 °C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG light chains on all sheep immunoglobulins.No reactivity is observed to: non-immunoglobulin sheep serum proteins IgG from human, mouse or rabbit.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Immunogen affinity purified donkey IgG.
Reconstitution:
For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
DCL3 (EC=3.1.26) is a ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs). Synonymes: Endoribonuclease Dicer homolog 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
LSD1 (Lesion simulating disease 1) monitors a superoxide-dependent signal and negatively regulates a plant cell death pathway. contains zinc-finger motifs. LSD1 negatively regulates a basal defense pathway that can act upstream or independently of both NIM1/NPR1 function and SA accumulation. Alternative names: CHILLING SENSITIVE 4, CHS4.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4 C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica olereacea, Pisum sativum Species of your interest not listed? Contact us
SRP43 (Signal recognition peptide 43) is a part of the chloroplast signal recognition particle pathway, required for post-translational targeting of proteins into the thylakoid membrane but seems to be dispensable for co-translational targeting with a translating ribosome present. The protein acts as a highly specific chaperone for LHCPs, preventing aggregation and being able to dissolve aggregates. Alternative names: Chloroplast signal recognition peptide 43, CPSRP43, CAO, CHAOS
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
no confirmed exceptions from predicted reactivity are currently known
Selected references:
To be added when available, antibody released in June 2023.
UniProt number:
O22265
TAIR number:
AT2G47450
Research area:
Arabidopsis thaliana antibodies
Code:
Photo20
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