Ultra fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification. Suitable for UNG treatment to prevent cross-contamination.
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) analyze 1-5 targets in 1 reaction mix with dUTP and UNG available to prevent carry-over contamination compatible with most cyclers different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start, and faster extension rates compared to the wild-type Taq DNA polymerase. qPCR buffer: Includes 16.5 mM MgCl2 (1x PCR solution 3.3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR
Ultra-fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification.
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) analyze 1-5 targets in 1 reaction mix with dUTP and UNG available to prevent carry-over contamination compatible with most cyclers different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start, and faster extension rates compared to the wild-type Taq DNA polymerase. qPCR buffer: Includes 15 mM MgCl2 (1x PCR solution 3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), additives that maximize efficiency of PCR Reference dye: ROX dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR
Ultra-fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification.
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) analyze 1-5 targets in 1 reaction mix with dUTP and UNG available to prevent carry-over contamination compatible with most cyclers different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
ROX dye: If ROX, Texas Red or similar dye is used as one of the fluorophores, ROX passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available.
Ultra fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification. Suitable for UNG treatment to prevent cross-contamination.
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) analyze 1-5 targets in 1 reaction mix with dUTP and UNG available to prevent carry-over contamination compatible with most cyclers different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase. qPCR buffer: Includes 16.5 mM MgCl2 (1x PCR solution 3.3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR
Extremely fast and highly sensitive dye-based qPCR that includes novel in-silico designed SolisFAST® DNA Polymerase Mix for most demanding applications
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using SolisGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases. It has two versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low ROX level for signal normalization. SolisGreen® qPCR mixes are suitable for commercial and diagnostic applications and require no additional licensing.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) bright and sensitive SolisGreen® dye suitable for commercial applications compatible with most cyclers, except high ROX different product versions: no ROX and ROX reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.
qPCR buffer: Includes 7.5 mM MgCl2 (1X PCR solution 1.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), stabilizers and enhancers that maximize efficiency of PCR reaction
Fluorescent dye: SolisGreen® is a nucleic acid dye detected in the FAM or SYBR® Green I channel
Extremely fast and highly sensitive dye-based qPCR that includes novel in-silico designed SolisFAST® DNA Polymerase Mix for most demanding applications
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using SolisGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases. It has two versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low ROX level for signal normalization. SolisGreen® qPCR mixes are suitable for commercial and diagnostic applications and require no additional licensing.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) bright and sensitive SolisGreen® dye suitable for commercial applications compatible with most cyclers, except high ROX different product versions: no ROX and ROX reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.
qPCR buffer: Includes 7.5 mM MgCl2 (1X PCR solution 1.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), stabilizers and enhancers that maximize efficiency of PCR reaction
Reference dye: ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR
Fluorescent dye: SolisGreen® is a nucleic acid dye detected in the FAM or SYBR® Green I channel
Cookies:
X
We use cookies to help personalise and improve your web experience.
By using our website you consent to our use of cookies, some of which may have already been set on your device.
View our Cookie Policy to learn more.
