A ready-to-use 5X-concentrated solution for High Resolution Melt (HRM) analysis of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection. The mix has two versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
excellent resolution in HRM assays allows detection of DNA sequence variations contains sensitive EvaGreen® dye compatible with most cyclers different product versions: no ROX, ROX reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2, 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Bovine serum albumin (BSA) to enhance qPCR reaction
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
ROX dye: If ROX, Texas Red or similar dye is used as one of the fluorophores, ROX passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available.
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12.5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Bovine serum albumine (BSA) to enhance qPCR reaction
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Background Info:
A ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 4 targets in one reaction with enhanced amplification of GC-rich regions. It contains dUTPs to prevent carry-over contamination when used with UNG treatment, and has three versions to match different instruments and assay requirements. ROX-mix is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
analyze 1-4 targets in 1 reaction high specificity and sensitivity robust amplification of GC-rich targets contains dUTP to prevent carry-over contamination when used in combination with UNG compatible with most cyclers (see table on page 17) different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix
Purple dye: If Cy5 or similar dye is used as one of the fluorophores, Purple passive reference dye might interfere with the signal. A version without passive reference or with ROX reference dye is available
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix is optimised for sensitive detection of up to 2 targets in one reaction. It has three versions to match different instruments. ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. Capillary-mix is optimised for capillary based systems.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for assays with 1-2 targets high specificity and sensitivity compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
5x Probe qPCR buffer with 15 mM MgCl2: 1x PCR solution 3 mM MgCl2
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Internal reference based on ROX dye
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