A chemically modified hot-start version of the thermostable Taq DNA polymerase FIREPol®. This enzyme is activated only by pre-incubation at 95°C, preventing any unspecific polymerase activity at lower temperatures during reaction set-up.
Background Info:
Chemically modified hot-start version of the thermostable Taq DNA polymerase FIREPol®. This enzyme is activated only after heat treatment at 95°C which prevents any unspecific polymerase activity at lower temperatures during reaction set-up. HOT FIREPol® DNA polymerase is supplied with 2 reaction buffers, 25 mM MgCl? and an additive for difficult templates. HOT FIREPol® 10x Buffer B2 contains non-ionic detergent suppressing inhibitory effects of the trace of DNA extraction buffers and enhancing PCR yield and efficiency.
Product Type:
Endpoint PCR
Format:
Product kit
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
increased specificity and sensitivity reduced primer dimer formation suitable for TA cloning reaction buffer with and without detergent included Solution S included in a separate vial for GC-rich templates MgCl? included in a separate vial reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase (5 U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers. HOT FIREPol® 10x Buffer B1 (without Mg2+ and detergent): 0.7 M Tris-HCl, 0.175 M (NH4)2SO4. HOT FIREPol® 10x Buffer B2 (without Mg2+): 0.7 M Tris-HCl, 0.175 M (NH4)2SO4, 0.2% w/v Tween-20. HOT FIREPol® 10x Buffer B2 contains non-ionic detergent suppressing inhibitory effects of the trace of DNA extraction buffers and enhancing PCR yield and efficiency. 25 mM MgCl2 10x Solution S is an additive that facilitates the amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined final concentration (1x, 2x or 3x solution). 10x Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplification occurs.
Vials included in the kit:
HOT FIREPol® DNA Polymerase 1x1000 U | 200 µl 10x Solution S 1x0.5 ml 25 mM MgCl2 1x5 ml HOT FIREPol® 10x Buffer B1 1x5 ml HOT FIREPol® 10x Buffer B2 1x5 ml
A chemically modified hot-start version of the thermostable Taq DNA polymerase FIREPol®. This enzyme is activated only by pre-incubation at 95°C, preventing any unspecific polymerase activity at lower temperatures during reaction set-up.
Background Info:
Chemically modified hot-start version of the thermostable Taq DNA polymerase FIREPol®. This enzyme is activated only after heat treatment at 95°C which prevents any unspecific polymerase activity at lower temperatures during reaction set-up. HOT FIREPol® DNA polymerase is supplied with 2 reaction buffers, 25 mM MgCl? and an additive for difficult templates. HOT FIREPol® 10x Buffer B2 contains non-ionic detergent suppressing inhibitory effects of the trace of DNA extraction buffers and enhancing PCR yield and efficiency.
Product Type:
Endpoint PCR
Format:
Product kit
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
increased specificity and sensitivity reduced primer dimer formation suitable for TA cloning reaction buffer with and without detergent included Solution S included in a separate vial for GC-rich templates MgCl? included in a separate vial reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase (5 U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers. HOT FIREPol® 10x Buffer B1 (without Mg2+ and detergent): 0.7 M Tris-HCl, 0.175 M (NH4)2SO4. HOT FIREPol® 10x Buffer B2 (without Mg2+): 0.7 M Tris-HCl, 0.175 M (NH4)2SO4, 0.2% w/v Tween-20. HOT FIREPol® 10x Buffer B2 contains non-ionic detergent suppressing inhibitory effects of the trace of DNA extraction buffers and enhancing PCR yield and efficiency. 25 mM MgCl2 10x Solution S is an additive that facilitates the amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined final concentration (1x, 2x or 3x solution). 10x Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplification occurs.
Vials included in the kit:
HOT FIREPol® DNA Polymerase 1x500 U | 100 µl 10x Solution S 1x0.1 ml 25 mM MgCl2 1x2.5 ml HOT FIREPol® 10x Buffer B1 1x2.5 ml HOT FIREPol® 10x Buffer B2 1x2.5 ml
A chemically modified hot-start version of the thermostable Taq DNA polymerase FIREPol®. This enzyme is activated only by pre-incubation at 95°C, preventing any unspecific polymerase activity at lower temperatures during reaction set-up.
Background Info:
Chemically modified hot-start version of the thermostable Taq DNA polymerase FIREPol®. This enzyme is activated only after heat treatment at 95°C which prevents any unspecific polymerase activity at lower temperatures during reaction set-up. HOT FIREPol® DNA polymerase is supplied with 2 reaction buffers, 25 mM MgCl? and an additive for difficult templates. HOT FIREPol® 10x Buffer B2 contains non-ionic detergent suppressing inhibitory effects of the trace of DNA extraction buffers and enhancing PCR yield and efficiency.
Product Type:
Endpoint PCR
Format:
Product kit
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
increased specificity and sensitivity reduced primer dimer formation suitable for TA cloning reaction buffer with and without detergent included Solution S included in a separate vial for GC-rich templates MgCl? included in a separate vial reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA Polymerase (5 U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers. HOT FIREPol® 10x Buffer B1 (without Mg2+ and detergent): 0.7 M Tris-HCl, 0.175 M (NH4)2SO4. HOT FIREPol® 10x Buffer B2 (without Mg2+): 0.7 M Tris-HCl, 0.175 M (NH4)2SO4, 0.2% w/v Tween-20. HOT FIREPol® 10x Buffer B2 contains non-ionic detergent suppressing inhibitory effects of the trace of DNA extraction buffers and enhancing PCR yield and efficiency. 25 mM MgCl2 10x Solution S is an additive that facilitates the amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined final concentration (1x, 2x or 3x solution). 10x Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplification occurs.
Vials included in the kit:
HOT FIREPol® DNA Polymerase 1x100 U | 20 µl 10x Solution S 1x0.1 ml 25 mM MgCl2 1x0.5 ml HOT FIREPol® 10x Buffer B1 1x0.5 ml HOT FIREPol® 10x Buffer B2 1x0.5 ml
FIREPol® is a highly processive, thermostable Taq DNA polymerase with unique 30-day stability at room temperature.
Background Info:
A genetically modified thermostable Taq DNA polymerase that provides robust and reproducible results. FIREPol® DNA polymerase is supplied with 2 reaction buffers, 25 mM MgCl2 and an additive for difficult templates. FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency.
Product Type:
Endpoint PCR
Format:
Product kit
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
robust amplification for routine applications suitable for templates up to 5 kb suitable for TA cloning reaction buffers with and without detergent included Solution S included in a separate vial for GC-rich templates MgCl2 included in a separate vial reaction set-up and shipment without ice
Mix/Kit Components:
FIREPol® DNA Polymerase (5 U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers. FIREPol® 10x Buffer B (without Mg2+ ): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20. FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency. FIREPol® 10x Buffer BD (without Mg2+ and detergent): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4. 25 mM MgCl2 10x Solution S is an additive that facilitates the amplification of difficult templates (e.g. GC-rich DNA templates).
Vials included in the kit:
FIREPol® DNA Polymerase 1x2000 U | 400 µl 10x Reaction Buffer B 2x5 ml 10x Reaction Buffer BD 2x5 ml 10x Solution S 1x0.5 ml 25 mM MgCl2 2x5 ml
FIREPol® is a highly processive, thermostable Taq DNA polymerase with unique 30-day stability at room temperature.
Background Info:
A genetically modified thermostable Taq DNA polymerase that provides robust and reproducible results. FIREPol® DNA polymerase is supplied with 2 reaction buffers, 25 mM MgCl2 and an additive for difficult templates. FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency.
Product Type:
Endpoint PCR
Format:
Product kit
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
robust amplification for routine applications suitable for templates up to 5 kb suitable for TA cloning reaction buffers with and without detergent included Solution S included in a separate vial for GC-rich templates MgCl2 included in a separate vial reaction set-up and shipment without ice
Mix/Kit Components:
FIREPol® DNA Polymerase (5 U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers. FIREPol® 10x Buffer B (without Mg2+ ): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20. FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency. FIREPol® 10x Buffer BD (without Mg2+ and detergent): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4. 25 mM MgCl2 10x Solution S is an additive that facilitates the amplification of difficult templates (e.g. GC-rich DNA templates).
Vials included in the kit:
FIREPol® DNA Polymerase 1x1000 U | 200 µl 10x Reaction Buffer B 1x5 ml 10x Reaction Buffer BD 1x5 ml 10x Solution S 1x0.5 ml 25 mM MgCl2 1x5 ml
FIREPol® is a highly processive, thermostable Taq DNA polymerase with unique 30-day stability at room temperature.
Background Info:
A genetically modified thermostable Taq DNA polymerase that provides robust and reproducible results. FIREPol® DNA polymerase is supplied with 2 reaction buffers, 25 mM MgCl2 and an additive for difficult templates. FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency.
Product Type:
Endpoint PCR
Format:
Product kit
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
robust amplification for routine applications suitable for templates up to 5 kb suitable for TA cloning reaction buffers with and without detergent included Solution S included in a separate vial for GC-rich templates MgCl2 included in a separate vial reaction set-up and shipment without ice
Mix/Kit Components:
FIREPol® DNA Polymerase (5 U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers. FIREPol® 10x Buffer B (without Mg2+ ): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20. FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency. FIREPol® 10x Buffer BD (without Mg2+ and detergent): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4. 25 mM MgCl2 10x Solution S is an additive that facilitates the amplification of difficult templates (e.g. GC-rich DNA templates).
Vials included in the kit:
FIREPol® DNA Polymerase 1x500 U | 100 µl 10x Reaction Buffer B 1x2.5 ml 10x Reaction Buffer BD 1x2.5 ml 10x Solution S 1x0.1 ml 25 mM MgCl2 1x2.5 ml
FIREPol® is a highly processive, thermostable Taq DNA polymerase with unique 30-day stability at room temperature.
Background Info:
A genetically modified thermostable Taq DNA polymerase that provides robust and reproducible results. FIREPol® DNA polymerase is supplied with 2 reaction buffers, 25 mM MgCl2 and an additive for difficult templates. FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency.
Product Type:
Endpoint PCR
Format:
Product kit
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
robust amplification for routine applications suitable for templates up to 5 kb suitable for TA cloning reaction buffers with and without detergent included Solution S included in a separate vial for GC-rich templates MgCl2 included in a separate vial reaction set-up and shipment without ice
Mix/Kit Components:
FIREPol® DNA Polymerase (5 U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers. FIREPol® 10x Buffer B (without Mg2+ ): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20. FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency. FIREPol® 10x Buffer BD (without Mg2+ and detergent): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4. 25 mM MgCl2 10x Solution S is an additive that facilitates the amplification of difficult templates (e.g. GC-rich DNA templates).
Vials included in the kit:
FIREPol® DNA Polymerase 1x100 U | 20 µl 10x Reaction Buffer B 1x0.5 ml 10x Reaction Buffer BD 1x0.5 ml 10x Solution S 1x0.1 ml 25 mM MgCl2 1 0.5 ml
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