Major 12S seed storage protein CRC (globulin) is synthesized on the endoplasmic reticulum as precursor and then transported to storage vacuoles, where it is processed at a conserved Asn-Gly peptide bond by an asparaginyl endopeptidase to produce two mature polypeptides referred to as alpha and beta subunits that are joined together by a disulfide bond, Phosphorylated in seeds on some Tyr residues in response to abscisic acid (ABA), 12S CRC protein is cleaved to alpha and beta chains, Cellular localisation: vacuole,Alternative protein names: Cruciferin 3, Cruciferin C Legumin-type globulin Cruciferin1, Legumin-type globulin storage protein CRU1,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml in PBS, 50% glycerol, Filter sterilized, No preservative or carrier added,
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us, tech@nktscientific.com
Immunogen:
Native, 12S globulin alpaha subunit purified from Arabidopsis thaliana and excised from SDS-PAGE gel, UniProt: Q96318, TAIR: At4g28520
Applications:
ELISA (ELISA), Immunolocalisation by electron microscopy (IL), Immunohistochemistry (IHC), Western blot (WB)
assay dependent (ELISA), 1: 50 (IL by electron microscopy), 1: 100 (IHC), 1: 3000 - 1: 10 000 (WB)
Purity:
Total IgG, purified on Protein A/G
Related products:
collection of antibodies to vacuolar proteins
Molecular Weight:
58 | 30 kDa (alpha subunit)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Shirakawa et al, (2014), CONTINUOUS VASCULAR RING (COV1) is a trans-Golgi network-localized membrane protein required for Golgi morphology and vacuolar protein sorting, Plant Cell Physiol, 2014 Apr;55(4):764-72,doi: 10,1093/pcp/pct195, (Immunohistochemistry, Western blot)Li et al, MAG2 and three MAG2-INTERACTING PROTEINs form an ER-localized complex to facilitate storage protein transport in Arabidopsis thaliana, Plant J, 2013 Dec;76(5):781-91,doi: 10,1111/tpj,12347, (Immunolocalisation by electron microscopy, Western blot)
GRF (General regulatory factor 2) or 14,3,3's are 30KDa proteins involved in protein interactions with target proteins containing phosphorylated target sites, Functions of 14,3,3's include acting as adaptors or scaffolds, stimulating protein-protein interaction, altering target protein activity, causing conformational changes of target proteins, regulating subcellular localisation and also facilitating transport (for example nuclear import/export and transport in the endomembrane system), A variety of apparently unrelated biological activities, including a role in development and signal transduction - brassinosteroid mediated signaling pathway,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
This product can be sold containing proclin if requested,
Application Details:
1: 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water,
Related products:
AS08 317 | 14,3,3C | 14,3,3-like protein C, rabbit antibody specific to Hordeum vulgare Collection of antibodies to proteins involved in signal transduction
Molecular Weight:
20-28 kDa (depending upon an isoform)
Not reactive in:
This antibody does not bind to 14-3-3-like protein D of Nicotiana tabacum,
Selected references:
Gupta and Shaw (2020), Biochemical and molecular characterisations of salt tolerance components in rice varieties tolerant and sensitive to NaCl: the relevance of Na+ exclusion in salt tolerance in the species , Funct Plant Biol, 2020 Jul 30,doi: 10,1071/FP20089 Dongxu et al, (2020), Magnesium reduces cadmium accumulation by decreasing the nitrate reductase-mediated nitric oxide production in Panax notoginseng roots, Journal of Plant Physiology, Available online 7 February 2020, 153131Pertl-Obermeyer et al, (2018), Dissecting the subcellular membrane proteome reveals enrichment of H+ (co-)transporters and vesicle trafficking proteins in acidic zones of Chara internodal cells, PLoS One, 2018 Aug 29;13(8):e0201480, doi: 10,1371/journal,pone,0201480, Obroucheva (2017), Participation of Plasma Membrane H+-ATPase in Seed Germination, Internat, J, of Cell Science & Molecular Biol, Vol, 2 Issue 3, DOI : 10,19080/IJCSMB,2017,02,555589,Barkla et al, (2016), Single-cell-type quantitative proteomic and ionomic analysis of epidermal bladder cells from the halophyte model plant Mesembryanthemum crystallinum to identify salt-responsive proteins, BMC Plant Biol, 2016 May 10;16(1):110, doi: 10,1186/s12870-016-0797-1,
Special application note:
This antibody is recognizing recombiant GRF of Lilium longiflorum Lil1433_0 accession: AF191746, Lil1433_2, accession: EF397608 and recombinant GRF1,2,3,5 and 6 of Arabidopsis thaliana: GRF1 14-3-3 chi (At4g09000,1), GRF2 14-3-3 omega (At1g78300,1), GRF3 14-3-3 psi (At5g38480,1), GRF5 14-3-3 upsilon (At5g16050,1), GRF6 14-3-3 lambda (At5g10450,2), GRF8 14-3-3 kappa (At5g65430,1), GRF11 14-3-3 omicron (At1g34760,1),There is also very weak reaction to Physcomitrella patens Pp14-3-3 Pp1s 73_133V6 (closest homolog to AtGRF6) and Chlamydomonas reinhardtii Cr 14-3-3 Cre 12,g559250 (closest homolog to AtGRF6),
2b protein [Cucumber mosaic virus] is one of the first identified suppressors that could inhibit post-transcriptional gene silencing (PTGS), but with little or no effect on miRNA functions, CMV 2b protein also interferes with miRNA pathways, eliciting developmental anomalies,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
2b protein [Cucumber mosaic virus]
Immunogen:
Recombinant 2b protein [Cucumber mosaic virus] Protein accession number: NP_619631,
2S seed storage protein 3, one of major seed storage proteins is synthesized on the endoplasmic reticulum as precursor and then transported to storage vacuoles, where it is processed by an asparaginyl endopeptidase to produce two mature polypeptides referred to as large and small subunits which are linked by disulfide bonds, Subcellular localisation: vacuole,Alternative names: 2S albumin storage protein, NWMU2-2S albumin 3
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml in PBS, 50% glycerol, Filter sterilized, No preservative or carrier added,
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Capsella rubella Species of your interest not listed? Contact us, tech@nktscientific.com
Immunogen:
Conjugated peptide, derived of Arabidopsis thaliana 2S3 large subunit, UniProt: P15459, TAIR: At4g27160
Applications:
ELISA (ELISA), Immunolocalisation by electron microscopy (IL), Western blot (WB)
collection of antibodies to membrane transport system proteins
Molecular Weight:
18, 7 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Takagi et al, (2013), MAIGO5 functions in protein export from Golgi-associated endoplasmic reticulum exit sites in Arabidopsis, Plant Cell , 2013 Nov;25(11):4658-75, doi: 10,1105/tpc,113,118158, (Immunolocalisation by electron microscopy, Western blot, Arabidopsis thaliana)
2S seed storage protein 3, one of major seed storage proteins is synthesized on the endoplasmic reticulum as precursor and then transported to storage vacuoles, where it is processed by an asparaginyl endopeptidase to produce two mature polypeptides referred to as large and small subunits which are linked by disulfide bonds, Subcellular localisation: vacuole,Alternative names: 2S albumin storage protein, NWMU2-2S albumin 3
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml in PBS, 50% glycerol, Filter sterilized, No preservative or carrier added,
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us, tech@nktscientific.com
Immunogen:
Conjugated peptide, derived from N-propeptide of Arabidopsis thaliana 2S3P, UniProt: P15459, TAIR: At4g27160
Applications:
ELISA (ELISA), Immunolocalisation by electron microscopy (IL), Western blot (WB)
collection of antibodies to membrane transport system proteins
Molecular Weight:
18,7 | 17-20 kDa (2S albumin precursors)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Shirakawa et al, (2014), CONTINUOUS VASCULAR RING (COV1) is a trans-Golgi network-localized membrane protein required for Golgi morphology and vacuolar protein sorting, Plant Cell Physiol, 2014 Apr;55(4):764-72, doi: 10,1093/pcp/pct195, (Western blot, Arabidopsis thaliana) Li et al, (2006), MAIGO2 is involved in exit of seed storage proteins from the endoplasmic reticulum in Arabidopsis thaliana, Plant Cell, 2006 Dec;18(12):3535-47, doi: 10,1105/tpc,106,046151,( Immunolocalisation by electron microscopy, Western blot, Arabidopsis thaliana)
RNA polymerase I is a nuclear located DNA-dependent enzyme involved in RNA elongation and regulation of transcription, In yeast subunit A12,2 has been described as homologous to the Rpb9 subunit from polymerase II,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from the Arabidopsis thaliana A12,2 (At3g25940) protein sequence, This sequence is only weakly conserved in other eukaryotic sequences available in the databases,
This antibody is specific for A12,2 subunit of RNA polymerase I but NOT RNA polymerase II or IV from Arabidopsis thaliana
Application Details:
1 : 2000 (WB)
Purity:
Affinity purified serum in PBS, pH 7,4
Reconstitution:
For reconstitution add 143 µl of sterile water,
Related products:
Plant protein extraction buffer Secondary antibodies
Molecular Weight:
13,6 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Special application note:
This product has previously been labelled as anti-At3g25940 transcription factor S-II (TFIIS) domain-containing protein,Protocol for isolation of cytosolic and nuclear fractions can be found here,
aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity, Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii
Expected Species:
Acinetobacter baumannii, Enterobacter agglomerans, Klebsiella pneumoniae, Klebsiella oxytoca, Kluyvera ascorbata, Pseudomonas aeruginosa, Salmonella enterica subsp, enterica serovar Worthington, Staphylococcus aureus, Serratia sp,, Shigella flexneri Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from known aadA1 protein sequences
antibody collection for Arabidopsis thalianaPlant protein extraction bufferSecondary antibodies
Molecular Weight:
30 | 30 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Lauersen et a, (2018), Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii, Metab Eng, 2018 Jul 12;49:116-127, doi: 10,1016/j,ymben,2018,07,005,
Special application note:
This product can be sold containing ProClin if requested,
aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity, Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection, This antibody is directly conjugated to ALP,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid, conjugated to ALP
Storage Temp:
Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years,
Host Animal:
Rabbit
Species Reactivity:
aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii
aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity, Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection, This antibody is directly conjugated to HRP,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid, conjugated to HRP
Storage Temp:
Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years,
Host Animal:
Rabbit
Species Reactivity:
aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii
Abscisic acid (ABA) is a plant hormone involved in different physiological responses as stimulation of the closure of stomata (water stress brings about an increase in ABA synthesis), iInhibition of shoot growth, and many others, ABA shown to have both inhibitory as well as many promoting functions,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid containing 50% glycerol
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Abscisic acid (C1) in Arabidopsis thaliana, Eucalyptus globulus, Petunia hybrida L,, Pinus radiata, Populus trichocarpa
Expected Species:
Abscisic acid (C1)
Immunogen:
BSA-conjugated abscisic acid (C1) via C1 carboxyl group
The antibody will recognize either the ABA conjugated to glucose ester (ABA-GE) or the ABA precursor: abscisic acid aldehyde, ABA aldehyde is however not usually present in plant tissue similarly to ABA alcohol which is also reactive, The antibodies will predominantly recognize only ABA and its glucosylester,
Application Details:
The optimal working dilution should be determined by the investigator,
Purity:
Affinity purified serum in PBS, pH 7,4
Related products:
AS09 422 | Anti-ABA | abscisic acid (C1) (quantity 1mg), rabbit antibodiesAS09 447 | Anti-ABA | abscisic acid (C1) (quantity 5mg), rabbit antibodiescollection of antibodies to other plant hormonesSecondary antibodies
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Wojciechowska et al, (2020), Abscisic Acid and Jasmonate Metabolisms Are Jointly Regulated During Senescence in Roots and Leaves of Populus trichocarpa, Int J Mol Sci , 21 (6) Dinis et al, (2018), Kaolin modulates ABA and IAA dynamics and physiology of grapevine under Mediterranean summer stress, J Plant Physiol, 2018 Jan;220:181-192, doi: 10,1016/j,jplph,2017,11,007, Kovaleva et al, (2017), ABA and IAA control microsporogenesis in Petunia hybrida L, Protoplasma, 2017 Nov 13, doi: 10,1007/s00709-017-1185-x, Escandón et al, (2016), Integrated physiological and hormonal profile of heat-induced thermotolerance in Pinus radiata, Tree Physiol, 2016 Jan;36(1):63-77, doi: 10,1093/treephys/tpv127, Epub 2016 Jan 12,Ondzighi-Assoume et al, (2016), Environmental Nitrate Stimulates Root Tip Abscisic Acid Accumulation via Release from Inactive Stores, Plant Cell, 2016 Feb 17, pii: TPC2015-00946-RA,Jesus et al, (2015), Salicylic acid application modulates physiological and hormonal changes in Eucalyptus globulus under water deficit, Environ and Exp Botany, Volume 118, October 2015, Pages 56–66,Lacuesta et al, (2013), Immunolocalization of IAA and ABA in roots and needles of radiata pine (Pinus radiata) during drought and rewatering, Tree Physiol, May;33(5):537-49,
Special application note:
Useful references for immunolocalization work: DeWitte et al, (1999), Dynamics of cytokinins in apical shoot meristems of a day neutral tobacco during floral transition and flower formation, Plant Physiol, 119:111-121, Zavala et al, (1983), Localisation of a phytohormone using immunocytochemistry, J,Cell Biol, 97:1235-1239,
ABF3 (Abscisic acid-insensitive 5-like 6) binds to the ABA-responsive element (ABRE) and mediates stress-responsive ABA signaling,Alternative names: Abscisic acid responsive elements-binding factor 3, ABRE-binding factor 3, Dc3 promoter-binding factor 5, AtDPBF5, bZIP transcription factor 37, AtbZIP37
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
ABI1 is a key component and repressor of the abscisic acid (ABA) signaling pathway, It regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane, drought-induced resistance and rhizogenesis, response to glucose, high light stress, seed germination and inhibition of vegetative growth, Expressed in seeds and seedlings, Confined to lateral root caps and columella cells in roots, Induced by low temperature, drought, high salt, ABA and ethylene, Activates/represses SnRK2,6/SRK2E/OST1 in response to ABA-dependent stimuli, Alternative names: ABI1, ABA INSENSITIVE 1, AtABI1, Protein phosphatase 2C 56, AtPP2C56, PP2C56,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide, derived from Arabidopsis thaliana ABI1 sequence UniProt: P49597, TAIR: AT4G26080, Chosen peptide is not present in AtABI2,
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Mitula et al, (2015), Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway, Plant Cell Physiol, 2015 Dec;56(12):2351-67, doi: 10,1093/pcp/pcv146, Epub 2015 Oct 6,
Special application note:
It is of crucial importance to chose a material in which ABI1 protein is highly expressed like seeds or senescent leaf, This protein could not be detected using this antibody in plants grown under optimal conditions, The antibody detects both, recombinant and endogenous ABI1 protein,
Protein Abscisic acid-insensitive 2 (ABI2) is a represor of the ABA signaling pathway, regulating various ABA responses like: stomatal closure, osmotic water permability of the plasma membrane, high light stress, response to glucose, seed germination and inhibition of vegetative growth, Involved in acquired thermotolerance, Alternative names: ABA INSENSITIVE 2, ABI2, ATABI2, Protein phosphatase 2C 77, AtPP2C77, At5g57050, MHM17_19, MHM17,19, PP2C ABI2,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7,4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from N-terminus of Arabidopsis thaliana ABI2 sequence, UniProt: O04719, ,TAIR: AT5G57050 chosen peptide is not conserved in ABI1
ABI2 antibodies recognize recombiant StrepTag-ABI2, ABI2-GST, His-ABI2, ABI2 protein is easily degraded therefore extraction buffer needs to contain protease inhibotors, example of such inhibitor coctail can be found here,To detect endogenous ABI2 plant material needs to be subjected to stress before harvesting,
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Mitula et al, (2015), Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway, Plant Cell Physiol, 2015 Dec;56(12):2351-67, doi: 10,1093/pcp/pcv146, Epub 2015 Oct 6,
ABI4 (Abscisic acid insensitive 4) is a transcription factor involved in the regulation of gene expression by stress factors, Confers sensitivity to abscisic acid (ABA) and regulates the ABA signaling pathway during seed germination upon nitrate-mediated lateral root inhibition, in hexokinase-dependent sugar responses (including feed-back regulation of photosynthesis and mobilization of storage lipid during germination), and in response to osmotic stress mediated by NaCl, KCl or mannitol, Plays a role in sucrose sensing or signaling, especially at low fluence far red light, ABI4 gene is expressed most abundantly in developing sliliques and to a lesser degree in seedlings, Alternative names: ABSCISIC ACID INSENSITIVE 4, ABI4, GLUCOSE INSENSITIVE 6, GIN6, IMPAIRED SUCROSE INDUCTION 3, ISI3, SALOBRENO 5,SAN5, SUCROSE UNCOUPLED 6, SUN6, SUGAR INSENSITIVE 5, SIS5, ERF ABI4, Ethylene-responsive transcription factor ABI4, ERF052, At2g40220, T7M7_16, T7M7,16,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
ABI5 (abscisic acid insensitive 5) is involved in ABA-regulated gene expression during seed development and subsequent vegetative stage and acts as the major mediator of ABA repression of growth, Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters, Alternative names: ABI5, ABA INSENSITIVE 5, GIA1, GROWTH-INSENSITIVITY TO ABA 1, Dc3 promoter-binding factor 1, AtDPBF1, GROWTH-INSENSITIVITY TO ABA 1, bZIP transcription factor 39, AtbZIP39,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica oleracea Species of your interest not listed? Contact us
Immunogen:
Recombinant HIS-tagged, full length ABI5 in gel slice, of Arabidopsis thaliana UniProt: Q9SJN0-1, TAIR: At2g36270, overexpressed in E,coli
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Stone et al, (2006), KEEP ON GOING, a RING E3 ligase essential for Arabidopsis growth and development, is involved in abscisic acid signaling, Plant Cell, 2006 Dec;18(12):3415-28,
ABI5 (abscisic acid insensitive 5) is involved in ABA-regulated gene expression during seed development and subsequent vegetative stage and acts as the major mediator of ABA repression of growth, Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters, Alternative names: ABI5, ABA INSENSITIVE 5, GIA1, GROWTH-INSENSITIVITY TO ABA 1, Dc3 promoter-binding factor 1, AtDPBF1, GROWTH-INSENSITIVITY TO ABA 1, bZIP transcription factor 39, AtbZIP39,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus, Populus trichocarpa Species of your interest not listed? Contact us
ABI5 protein is present in very low levels therefore specific material should be used for analysis as well as chemiluminescence detection reagent in extreme low femtogram range, as AgriseraECLSuperBright,
Application Details:
1: 140 (IL), 1 : 200 (WB)
Purity:
Affinity purified serum in PBS, pH 7,4
Reconstitution:
For reconstitution add 100 µl of sterile water to each tube,
No confirmed exceptions from predicted reactivity are currently known,
Special application note:
MG132 is recommened to be added to extraction buffer as ABI5 is degraded by proteasome as well as homogenization with thiourea and bead beater, Protocol for protein extraction from seeds can be requested here,
ABP1 is an auxin receptor which regulates polar auxin transport, It is involved in the shade avoidance responsse, controls cell division and elongation, controls the size of a root meristem and madiates auxin responsivness, ABP1 promotes endocytosis via clathrin recruitment and restricts PIN internalization upon auxin binding via inhibition of clathrin-mediated endocytosis,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
ABP1 is an auxin receptor which regulates polar auxin transport, It is involved in the shade avoidance responsse, controls cell division and elongation, controls the size of a root meristem and madiates auxin responsivness, ABP1 promotes endocytosis via clathrin recruitment and restricts PIN internalization upon auxin binding via inhibition of clathrin-mediated endocytosis,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
AS16 3945 | Anti-ABP1 | Auxin-binding protein 1, rabbit antibodiesAS16 3946 | Anti-ABP1 | Auxin-binding protein 1 (maize), rabbit antibodiesAS16 3947 | Anti-ABP4 | Auxin-binding protein 4 (maize), rabbit antibodiesAS16 3948 | Anti-ABP1 | Auxin-binding protein 1, rabbit antibodiesAS16 3949 | Anti-ABP1 | Auxin-binding protein 1, rabbit antibodiesPlant protein extraction buffer Secondary antibodies
Molecular Weight:
18,4 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Brown & Jones (1994), Mapping the auxin-binding site of Auxin-binding protein, 1 J Biol Chem 269: 21138-21140, Jones & Herman (1993), KDEL-Containing Auxin-Binding Protein 1 is Secreted to the Plasma Membrane and Cell Wall, Plant Physiol, 101: 595-606, Jones et al, (1991) Red light-regulated growth, I, Changes in the abundance of indoleacetic acid and a 22-kilodalton auxin-binding protein in maize mesocotyl, Plant Physiol 97: 352-358
ABP1 is an auxin receptor which regulates polar auxin transport, It is involved in the shade avoidance responsse, controls cell division and elongation, controls the size of a root meristem and madiates auxin responsivness, ABP1 promotes endocytosis via clathrin recruitment and restricts PIN internalization upon auxin binding via inhibition of clathrin-mediated endocytosis,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
ACA2 (Calcium-transporting ATPase 2) is a magnesium-dependent enzyme ((EC:3,6,3,8), which catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the endoplasmic reticulum, Alternative name: Ca(2+)-ATPase isoform 2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
GST-fusion of ACA2 peptide, purified by SDS-PAGE of Arabidopsis thaliana ACA2 protein sequence, UniProt: O81108, TAIR: AT4G37640
ACA2 can be found in a good levels in: roots and flowers while the levels in leaves and siliques are very low, Harper et al, 1998,This antibody is recognizing both full length and trunctated forms,
Application Details:
1 : 10 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl, of sterile water,
Related products:
collection of antibodies for Arabidopsis thaliana
Molecular Weight:
110 | 110 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Hwang et al, (2000), Calmodulin activation of an endoplasmic reticulum-located calcium pump involves an interaction with the N-terminal autoinhibitory domain,Plant Physiol, 2000 Jan;122(1):157-68,Hwang et al, (2000), Calmodulin activation of an endoplasmic reticulum-located calcium pump involves an interaction with the N-terminal autoinhibitory domain,Plant Physiol, 2000 Jan;122(1):157-68,Harper et al, (1998), A novel calmodulin-regulated Ca2+-ATPase (ACA2) from Arabidopsis with an N-terminal autoinhibitory domain, J Biol Chem, 1998 Jan 9;273(2):1099-106, (this paper contains a blot of tissue specific expression of ACA2),Harper et al, (1998), A novel calmodulin-regulated Ca2+-ATPase (ACA2) from Arabidopsis with an N-terminal autoinhibitory domain, J Biol Chem, 1998 Jan 9;273(2):1099-106, (this paper contains a blot of tissue specific expression of ACA2)
ACCase subunit beta (acetyl-coenzyme A, carboxylase (subunit beta)) is a component of the acetyl coenzyme A carboxylase (ACC) complex, It is catalyzing carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT),
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
For reconstitution add 100 µl of sterile water,Lyophilized antibody can be stored at -20 or -80°C, Once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Aethionema grandiflorum, Barbarea verna, Brassica napus, Capsella bursa pastoris, Carica papaya, Cardamine impatiens, Crucihimalaya wallichii, Draba nemorosa, Lepidium virginicum, Lobularia maritima, Nasturtium officinale, Olimarabidopsis pumila, Pachycladon enysii, Raphanus sativus, Theobroma cacao Species of your interest not listed? Contact us
Immunogen:
Recombinant ACC of Arabidopsis thaliana UniProt: P56765, TAIR:ATCG00500
1-aminocyclopropane-1-carboxylate synthase (ACS) enzymes catalyze the conversion of S-adenosyl-L-methionine (SAM) into 1-aminocyclopropane-1-carboxylate (ACC), a direct precursor of ethylene, (EC:4,4,1,14), Synonymes: S-adenosyl-L-methionine methylthioadenosine-lyase 7,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
ACC synthase 8 belongs to a group fo enzymes which catalyze the conversion of S-adenosyl-L-methionine (SAM) into 1-aminocyclopropane-1-carboxylate (ACC), a direct precursor of ethylene, ACS8 is auxin inducible, Alternative names: 1-aminocyclopropane-1-carboxylate synthase 8, ACC synthase 8, S-adenosyl-L-methionine methylthioadenosine-lyase 8,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20°C or -80°C, avoid repeated freeze-thaw cycles, Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes,
Acd1 (accelerated cell death 1) EC=1,14,12,20 is an enzyme involved in chlorophyll breakdown, pheophorbide a oxygenase, This enzyme seems to induce cell death in Arabidopsis leaves in the dark, Synonymes:PaO, Lls1, lethal leaf-spot 1 homolog, pheide a oxygenase
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus, Solanum lycopersicum, Nicotiana tabacum Species of your interest not listed? Contact us
Immunogen:
Recombinant PaO from Arabidopsis thaliana Q9FYC2, At3g44880
This antibody works on total cell extracts and can be used as a senescence marker, Predicted size of Acd1 precursor protein is about 61 kD including the transit peptide, but it must be processed to a smaller size, Using fresh extracts is recommended to decrease possible cross-reaction with Rubisco,
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water,
Related products:
collection of antibodies to proteins involved in senescencePlant protein extraction buffer Secondary antibodies
Molecular Weight:
61 | 54 kDa
Not reactive in:
Pinus strobus
Selected references:
Kim et al, (2013), Mutation of the Arabidopsis NAC016 Transcription Factor Delays Leaf Senescence,'Plant Cell Physiol, Aug 21,Nagane et al, (2010), Involvement of AtNAP1 in thre reulation of chlorophyll degradation in Arabiopsis thaliana, Planta (4):939-949,Hirashima et al, (2009), Light-independent cell death induced by accumulation of pheophorbide a in Arabidopsis thaliana, Plant Cell Physiol, (4):719-729,
Special application note:
The protein level is moderately induced during dark-induced senescence,
Aconitase is a single subunit enzyme of the tricarboxylic acid cycle (or Krebs cycle) in the mitochondria, A cytosolic isoform is also part of the glyoxylate cycle, Aconitase catalyzes the dehydration / hydration of citrate to iso-citrate, via cis-aconitate as an intermediate, The reaction is facilitated by an iron-sulphur cluster in the active site of the enzyme, The iron-sulphur cluster is somewhat unstable, especially under oxidative stress, and loss of the cofactor leads to degradation of the protein,Alternative names: ACO, citrate hydro-lyase 1,2,3
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana ACO1,ACO2 and ACO3 isoforms, Brassica oleracea, Solanum lycopersicum
Expected Species:
Cucurbita maxima, Nicotiana tabacum, Ricinus communis, Solanum tuberosum, Vitis vinifera, Oryza sativa, Zea mays, Picea sitcHensis, Populus trichocarpa Species of your interest not listed? Contact us
Immunogen:
Arabidopsis ACO1 (AT4G35830, Q42560), codon 120 - 898 (C-terminus), was cloned in fusion with a N-terminal 6xHis tag, and over-expressed in E, coli, All recombinant protein accumulated in inclusion bodies, which were purified by centrifugation and solubilised in 6 M guanidine-HCl, The protein was refolded by dilution in 100 mM Tris-HCl 8,5, 10% (v/v) glycerol, 2 mM dithiothreitol, and concentrated prior to immunisation,
The antibody recognises all three Arabidopsis aconitase isoforms (ACO1, ACO2 and ACO3, see Bernard et al 2009), Possible differences in affinity have not been precisely quantified, Sensitivity threshold is between 2 and 10 ng for WB / ECL (see figure), Antibodies will recognize aconitase isoforms in denaturing and native gel electrophoresis,
Application Details:
1 : 5 000 -1 : 10 000 (WB), At higher concentrations the antibody binds aspecifically resulting in non-specific signals around 60 kDa, including Rubisco subunits,
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water,
Related products:
collection of antibodies to proteins located in mitochondriaPlant protein extraction bufferSecondary antibodies
Molecular Weight:
98 kDa, Note that ACO1, ACO2 and ACO3 cannot be distinguished in size by standard SDS-PAGE,
Arabidopsis expresses three highly similar aconitase isozymes (ACO1/ AT4G35830, ACO2/AT4G26970 and ACO3/AT2G05710), of which ACO1 is the cytosolic isoform, while ACO2 and ACO3 are predominantly located in the mitochondria (Arnaud et al 2007, Bernard et al 2009), The combined abundance and activity of the mitochondrial aconitases is about 3 times higher than the cytosolic pool (Bernard et al 2009),The Arabidopsis isoforms are more similar in amino acid sequence to mammalian iron-regulatory proteins (IRP-1) than to the mammalian and yeast mitochondrial aconitases,
ACO4 (1-aminocyclopropane-1-carboxylate oxidase 4) is an enzyme involved in the ethylene biosynthesis, Alternative names: ACC oxidase,Ethylene-forming enzyme,EFE,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth, Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence,
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C,Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes,
Host Animal:
Mouse
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Clonorchis sinensis, Cucurbita maxima, Cucumis sativus, Genlisea aurea, Glycine soja, Gossypium arboreum, Gossypium hirsutum, Litsea cubeba, Medicago truncatula, Narcissus tazetta var, chinensis, Phaseolus vulgaris, Platycodon grandiflorus, Paulownia tomentosa, Populus trichocarpa, Phaseolus vulgaris, Prunus avium, Ricinus communis, Solanum tuberosum, Striga asiatica, Theobroma cacao Species of your interest not listed? Contact us
Immunogen:
Full length recombinant Arabidopsis thaliana Actin-1 (ACT1) expressed in E,coli, Uniprot: P0CJ46
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Kandasamy, M,K, et al, (2012), Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists, Plant Cell, 24, 2012 May;24(5):2041-57, doi: 10,1105/tpc,111,095281Kandasamy, M,K, et al, (2001), One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation, Plant Cell, Jul;13(7):1541-54Kandasamy, M,K, et al, (1999), The late pollen-specific actins in angiosperms, Plant Journal, Jun;18(6):681-91,
Special application note:
This antibody is a total IgG fraction purified on a protein G column, It recognizes the epitope Asn79 of actins ACT1, 3, 4, 12,
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth, Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence,
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C,Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes,
Host Animal:
Mouse
Species Reactivity:
Arabidopsis thaliana, Nicotiana tabacum
Expected Species:
Actinidia deliciosa, Brachypodium sylvaticum, Brassica napus, Brassica oleracea, Cucumis sativus, Dionea muscipula, Euphorbia lathyris, Ficus microcarpa, Gentlisea aurea, Gossypium sp,, Hevea brasiliensis, Medicago sativa, Medicago truncatula, Oryza sativa subsp, japonica, Oxytropis ochrocephala, Panax notoginseng, Populus trichocarpa, Phaseolus vulgaris, Prunus avium, Striga asiatica, Theobrom cacao, Torenia fournieri, Trifolium pratense, Vaccinium ashei, Ziziphus jujuba Species of your interest not listed? Contact us
Immunogen:
Full length recombinant Arabidopsis thaliana Actin-2 (ACT2) expressed in E,coli, Uniprot: Q96292
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Kandasamy, M,K,, et al, (2012), Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists, Plant Cell May;24(5):2041-57, doi: 10,1105/tpc,111,095281,Kandasamy, M,K,, et al, (2001), One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation, Plant Cell, Jul;13(7):1541-54,Kandasamy, M,K,, et al, (1999), The late pollen-specific actins in angiosperms, Plant Journal, Jun;18(6):681-9,
Special application note:
This antibody is a total IgG fraction, purified on a protein G column, It recognizes actin isoforms ACT2, 8, and 11,
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth, Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence,
Product Type:
Antibody
Antibody Type:
Recombinant
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth, Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Chlamydomonas reinhardtii (too high background for this species)
Selected references:
Zhuang et al (2021), EGY3 mediates chloroplastic ROS homeostasis and promotes retrograde signaling in response to salt stress in Arabidopsis, Cell Rep, 2021 Jul 13;36(2):109384, doi: 10,1016/j,celrep,2021,109384, PMID: 34260941,Ngou et al, (2021) Mutual potentiation of plant immunity by cell-surface and intracellular receptors, Nature, 2021 Mar 10, doi: 10,1038/s41586-021-03315-7, Epub ahead of print, PMID: 33692545,?abuz et al, (2021) Phototropin interactions with SUMO proteins, Plant Cell Physiol, 2021 Feb 17:pcab027, doi: 10,1093/pcp/pcab027, Epub ahead of print, PMID: 33594440,Khajuria et al, (2020), Photochemical Efficiency Is Negatively Correlated With the ? 9- Tetrahydrocannabinol Content in Cannabis Sativa L, Plant Physiol Biochem, 2020 Apr 8;151:589-600, doi: 10,1016/j,plaphy,2020,04,003,Molnár et al, (2020), Nitro-oxidative Signalling Induced by Chemically Synthetized Zinc Oxide Nanoparticles (ZnO NPs) in Brassica Species, Chemosphere, 251, 126419
Special application note:
Antibody available in 3 various pack sizes: 50, 100 and 150 µl - Please inquire,This product can be sold containing ProClin if requested,
ADP-glucose pyrophosphorylase (AGPase) catalyses the first committed step in the synthesis of transient starch in chloroplasts and storage starch in amyloplasts in plants (Tetlow et al,, 2004), AGPase in higher plants is composed of two distinct subunits encoded by separate genes, forming an L2S2 heterotetramer, The large subunits (L) are modulators of allosteric activity, while the small subunits (S) are the catalytic subunits (Kim et al,, 2007), Synonymes:ADP-glucose pyrophosphorylase, ADP-glucose synthase, AGPase B, Alpha-D-glucose-1-phosphate adenyl transferase
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Cross reactivity of this antibody to Rubisco has been excluded using 2D gel electrophoresis,Antigen used to elicit this antbody has very poor conservation in large subunit of ADP-glucose pyrophosphorylase,
Application Details:
1 : 1000-1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water,
Related products:
other antibodies involved in carbohydrate metabolismPlant and algal protein extraction buffer Secondary antibodies
Molecular Weight:
49,4 | 52 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Ma et al, (2021) A plasma membrane transporter coordinates phosphate reallocation and grain filling in cereals, Nat Genet, 2021 Apr 29, doi: 10,1038/s41588-021-00855-6, Epub ahead of print, PMID: 33927398,Chang et al, (2020), Enhanced lipid productivity in AGP knockout marine microalga Tetraselmis sp, using a DNA-free CRISPR-Cas9 RNP method, Bioresource Technology,Volume 303, May 2020, 122932, Ancin et al, (2019), NTRC and Thioredoxin f Overexpression Differentially Induces Starch Accumulation in Tobacco Leaves,Plants (Basel), 2019 Nov 26;8(12), pii: E543, doi: 10,3390/plants8120543,Takahashi et al, (2019), Glutelin subtype-dependent protein localization in rice grain evidenced by immunodetection analyses, Plant Mol Biol, 2019 Mar 25, doi: 10,1007/s11103-019-00855-5,Deng et al, (2018), Comparative Proteome Analysis of Wheat Flag Leaves and Developing Grains Under Water Deficit, Front Plant Sci, 2018 Apr 10;9:425, doi: 10,3389/fpls,2018,00425, eCollection 2018,
Alcohol dehydrogenase (ADH) is an enzyme playing a crucial role in the fermentative metabolism in plants subjected to low oxygen stress, It is known to be synthesized preferentially under low oxygen conditions,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Allyl alcohol dehydrogenase of Nicotiana tabacum, accession 75206691 and in Chlamydomonas reinhardtii,
Selected references:
Ventura et al, (2020), Arabidopsis phenotyping reveals the importance of alcohol dehydrogenase and pyruvate decarboxylase for aerobic plant growth, Sci Rep, 2020 Oct 7;10(1):16669, doi: 10,1038/s41598-020-73704-x, PMID: 33028901; PMCID: PMC7542448,Gil-Monreal et al, (2019), ERF-VII transcription factors induce ethanol fermentation in response to amino acid biosynthesis-inhibiting herbicides, J Exp Bot, 2019 Aug 6, pii: erz355, doi: 10,1093/jxb/erz355,Bui et al, (2019), Conservation of ethanol fermentation and its regulation in land plants, J Exp Bot, 2019 Feb 28, pii: erz052, doi: 10,1093/jxb/erz052,De la Rosa et al, (2019), A dicistronic precursor encoding miR398 and the legume-specific miR2119 coregulates CSD1 and ADH1 mRNAs in response to water deficit, Plant Cell Environ, 2019 Jan;42(1):133-144, doi: 10,1111/pce,13209,Giuntoli et al, (2014), A trihelix DNA binding protein counterbalances hypoxia-responsive transcriptional activation in Arabidopsis, PLoS Biol, 2014 Sep 16;12(9):e1001950, doi: 10,1371/journal,pbio,1001950, eCollection 2014,
Special application note:
This product can be sold containing ProClin if requested,
AGB1 belongs to a family of proteins involved in signal transduction, They are acting like molecular switches when transmitting signals from the outside to the inside of cells,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brasica sp,, Cajanus cajan, Camelina sativa, Capsella rubella, Eutrema sp,, Cicer arietinum, Gossypium sp,, Medicago truncatula, Morus sp,, Cajanus cajan, Pisum sativum, Sesamum indicum, Solanum sp,, Tarenaya hassleriana, Theobroma cacao, Trifolium subterraneum Species of your interest not listed? Contact us
AGO10 is involved in miRNA binding, and in RNA-mediated posttranscriptional gene silencing (PTGS),
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
A, lyrata, B, napus, C, rubella, C, clementina, C, sinensis, E, salsugineum, G, arboreum, G, raimondii, N, benthamianaSpecies of your interest not listed? Contact us, tech@nktscientific.com
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AGO10 protein sequence, Uniprot: Q9XGW1, TAIR: AT5G43810
AGO expression may be cell/tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest, Seedlings can be used as a negative control, Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure,
Sun et al, (2021) The epigenetic factor FVE orchestrates cytoplasmic SGS3-DRB4-DCL4 activities to promote transgene silencing in Arabidopsis, Sci Adv, 2021 Aug 4;7(32):eabf3898, doi: 10,1126/sciadv,abf3898, PMID: 34348894; PMCID: PMC8336953,Sprunck et al, (2019), Elucidating small RNA pathways in Arabidopsis thaliana egg cells,
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC), This protein complex is responsible for the gene silencing (RNAi),
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest, Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure, Buffer for extraction of AGO proteins: Paudel et al, 2018, The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies, The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot,TCA acetone precipitation method
Brioudes et al, (2021) HASTY, the Arabidopsis EXPORTIN5 ortholog, regulates cell-to-cell and vascular microRNA movement, EMBO J, 2021 Aug 2;40(15):e107455, doi: 10,15252/embj,2020107455, Epub 2021 Jun 21, PMID: 34152631; PMCID: PMC8327949, (Immunoprecipitation)Schwenk et al, (2021) Uncovering a novel function of the CCR4-NOT complex in phytochrome A-mediated light signalling in plants, Elife, 2021 Mar 30;10:e63697, doi: 10,7554/eLife,63697, PMID: 33783355; PMCID: PMC8009681,Dunker, Lederer, and Weiberg, (2021), Plant ARGONAUTE Protein Immunopurification for Pathogen Cross Kingdom Small RNA Analysis, Bio-protocol 11(3): e3911, DOI: 10,21769/BioProtoc,3911,Dunker et al, (2020), Oomycete small RNAs bind to the plant RNA-induced silencing complex for virulence, Elife, 2020 May 22;9:e56096,doi: 10,7554/eLife,56096, Niedojad?o et al, (2020), Dynamic distribution of ARGONAUTE1 (AGO1) and ARGONAUTE4 (AGO4) in Hyacinthus orientalis L, pollen grains and pollen tubes growing in vitro, Protoplasma, 2020 Jan 8, doi: 10,1007/s00709-019-01463-2,
Special application note:
Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U,To detect AGO1 in Nicotiana benthamiana, please inquire,Recommended for detection of AGO1: extreme low femtogram range chemiluminescent detection reagent
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC), This protein complex is responsible for the gene silencing (RNAi),This antibody is directly conjugated to Alkaline Phosphatase (ALP),
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid, conjugated to ALP
Storage Temp:
Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest, Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure,The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies, The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot,TCA acetone precipitation method
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC), This protein complex is responsible for the gene silencing (RNAi),This antibody is directly conjugated to Biotin,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid, conjugated to biotin,
Storage Temp:
Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conugated N-terminal peptide of Arabidopsis thaliana AGO1 UniProt: O04379, TAIR:At1g48410,
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest, Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure,The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies, The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot,
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC), This protein complex is responsible for the gene silencing (RNAi),This antibody is directly conjugated to Horseradish Peroxidase (HRP),
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Nicotiana benthamiana
Expected Species:
Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest, Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure,The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies, The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot,
AGO2 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC), This protein complex is responsible for the gene silencing (RNAi), AGO2 is probably involved in antiviral RNA silencing,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Capsella rubella, Solanum tuberosum Species of your interest not listed? Contact us
AGO2 protein is strongly induced by stress, AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest, Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure, Antibody incubation should be done over night in 4°C, Use of material with enriched AGO2 levels is recommened,
Wang et al, (2019), The PROTEIN PHOSPHATASE4 Complex Promotes Transcription and Processing of Primary microRNAs in Arabidopsis, Plant Cell, 2019 Feb;31(2):486-501, doi: 10,1105/tpc,18,00556,Dalmadi et al, (2019), AGO-unbound cytosolic pool of mature miRNAs in plant cells reveals a novel regulatory step at AGO1 loading, Nucleic Acids Res, 2019 Aug 8, pii: gkz690, doi: 10,1093/nar/gkz690,You et al, (2019), FIERY1 promotes microRNA accumulation by suppressing rRNA-derived small interfering RNAs in Arabidopsis, Nat Commun, 2019 Sep 27;10(1):4424, doi: 10,1038/s41467-019-12379-z, (immunoprecipiation)
AGO4 (Argonaute 4) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC), This protein complex is responsible for the gene silencing (RNAi),
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tubes,
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest, Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure, Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca, 40 kDa,Binds endogenous siRNAs, preference for 24nt siRNAs with 5' A,Note that the AGO4 antibody reacts with the NEB prestained protein marker,
Application Details:
1 : 100 (ICC), 5 µg of antibody per 1 gram of a fresh tissue (IP),1 : 2000-1 : 5000 (WB)
NiedojadÃ??o et al, (2020), Dynamic distribution of ARGONAUTE1 (AGO1) and ARGONAUTE4 (AGO4) in Hyacinthus orientalis L, pollen grains and pollen tubes growing in vitro, Protoplasma, 2020 Jan 8, doi: 10,1007/s00709-019-01463-2,Sprunck et al, (2019), Elucidating small RNA pathways in Arabidopsis thaliana egg cells, http://dx,doi,org/10,1101/525956Yang et al, (2017), The developmental regulator PKL is required to maintain correct DNA methylation patterns at RNA-directed DNA methylation loci, Genome Biol, 2017 May 31;18(1):103, doi: 10,1186/s13059-017-1226-y,Li et al, (2016), Biogenesis of phased siRNAs on membrane-bound polysomes in Arabidopsis, Elife, 2016 Dec 12;5, pii: e22750, doi: 10,7554/eLife,22750,Zhai et al, (2015), A One Precursor One siRNA Model for Pol IV-Dependent siRNA Biogenesis, Cell, 2015 Oct 8;163(2):445-55, doi: 10,1016/j,cell,2015,09,032,Han et al, (2014), SUVR2 is involved in transcriptional gene silencing by associating with SNF2-related chromatin-remodeling proteins in Arabidopsis, Cell Res, 2014 Nov 25, doi: 10,1038/cr,2014,156, (coimmunoprecipitation)Zhang et al, (2013), DTF1 is a core component of RNA-directed DNA methylation and may assist in the recruitment of Pol IV, PNAS, May 14,Havecker et al, (2010) The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci, Plant Cell 22(2): 321-34,
AGO4 (Argonaute 4) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC), This protein complex is responsible for the gene silencing (RNAi),
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tubes,
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest, Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure, Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca, 40 kDa,Note that the AGO4 antibody reacts with the NEB prestained protein marker,
Application Details:
5 µg of antibody per 1 gram of a fresh tissue (IP), 1 : 100 (ICC), 1 : 4000 (WB)
Zhai et al, (2015), A One Precursor One siRNA Model for Pol IV-Dependent siRNA Biogenesis, Cell, 2015 Oct 8;163(2):445-55, doi: 10,1016/j,cell,2015,09,032,Han et al, (2014), SUVR2 is involved in transcriptional gene silencing by associating with SNF2-related chromatin-remodeling proteins in Arabidopsis, Cell Res, 2014 Nov 25, doi: 10,1038/cr,2014,156, (coimmunoprecipitation)Zhang et al, (2013), DTF1 is a core component of RNA-directed DNA methylation and may assist in the recruitment of Pol IV, PNAS, May 14,Havecker et al, (2010) The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci, Plant Cell 22(2): 321-34,
Special application note:
Binds endogenous siRNAs, preference for 24nt siRNAs with 5' A,
AGO5 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC), This protein complex is responsible for the gene silencing (RNAi), Probably involved in antiviral RNA silencing,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
peptide of Arabidopsis thaliana AGO5 UniProt: Q9SJK3, TAIR:AT2G27880
Applications:
Immunofluorescebce (IF), Immunoprecipitation (IP), Western blot (WB)
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest, Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure,
Application Details:
1 : 100 (IF), 5 ug per gram floral tissue (IP), 1 : 1500 (WB)
AGO6 (Argonaute 6) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC), This protein complex is responsible for the gene silencing (RNAi),
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana AGO6 UniProt: O48771 TAIR:At2g32940
Sprunck et al, (2019), Elucidating small RNA pathways in Arabidopsis thaliana egg cells, http://dx,doi,org/10,1101/525956 Havecker el al, (2010), The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci, Plant Cell, 2010 Feb;22(2):321-34, doi: 10,1105/tpc,109,072199,
Special application note:
There can be a reaction with NEB broad range prestained protein ladder depending upon secondary antibody used,AGO6 accumulates to lower levels in the nrpd1a mutant (Havecker at el, 2010), In addition, a non-specific band migrates to a similar location and thus protein was run on a 6% denaturing gel to obtain good separation,If other secondary antibody is used than in the figure below, cross-reacting pattern can be different,
AGO9 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC),
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AGO9 protein sequence UniProt: Q84YI4, TAIR:At5g21150,
Applications:
Immunolocalization, whole-mount (IL), Immunoprecipitation (IP), Western blot (WB)
AGO expression may be cell/tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest, Seedlings can be used as a negative control, Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure,
Application Details:
5 µg of antibody per 1 gram of a fresh tissue (IP), 1 : 10 000 (WB)
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Sprunck et al, (2019), Elucidating small RNA pathways in Arabidopsis thaliana egg cells, http://dx,doi,org/10,1101/525956Su et al, (2017), The THO Complex Non-Cell-Autonomously Represses Female Germline Specification through the TAS3-ARF3 Module, Curr Biol, 2017 Jun 5;27(11):1597-1609,e2, doi: 10,1016/j,cub,2017,05,021,Havecker et al, (2010) The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci, Plant Cell 22(2): 321-34,
AHB2 (Arabidopsis hemoglobin2) belongs to plant globin family and is a heme, iron and oxygen binding protein, Expressed in rosette leaves and young roots and induced by variety of environmental stresses, Synonymes: AHB2, ARABIDOPSIS HEMOGLOBIN 2, ARATH GLB2, ATGLB2, GLB2, HAEMOGLOBIN 2, HB2, HEMOGLOBIN 2, NON-SYMBIOTIC HAEMOGLOBIN 2, NSHB2,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Beta vulgaris, Brassica napus, Gossypium hirsutum, Solanum lycopersicum, Solanum tuberosum Species of your interest not listed? Contact us
AHK2 (Histidine kinase 2) is a cytokinin receptor, multi-pass membrane protein which transmits the stress signal to a downstream MAPK cascade, Alternative name: Authentic HIS-Kinase 2,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Nicotiana benthamiana, Vitis viniferaSpecies of your interest not listed? Contact us, tech@nktscientific.com
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AHK2 protein sequence, TAIR: AT5G35750, UniProt: Q9C5U2
AKIN10 (E,C,= 2,7,11,1) is a catalytic subunit of the putative trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants, Synonymes: AKIN alpha-2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from C-terminal part of Arabidopsis thaliana AKIN10 sequence UniProt: Q38997, TAIR: At3g01090
AKIN10 antibody works best when first the SnRK1 complex is immunorecipitated with this or another antibody and then anti-AKIN10 antibody is used to probe with, When this antibody is used against a lysate, the dominant rubisco protein can mask any immunoreactivity,
Application Details:
1: 50 (IL), 1 : 500 (WB)
Purity:
Affinity purified serum in PBS, pH 7,4
Reconstitution:
For reconstitution add 50 µl/tube of sterile water,
Related products:
AS09 460 | Anti-AKINB1 | SNF1-related protein kinase regulatory subunit beta-1, rabbit antibodiesAS09 462 | Anti-AKINB2 | SNF1-related protein kinase regulatory subunit beta-2, rabbit antibodiesAS15 2859 | Anti-AKIN ß3 | SNF1-related protein kinase regulatory subunit beta-3, rabbit antibodiesAS09 463 | Anti-AKIN beta gamma, rabbit antibodiesAS10 920 | Anti-AKIN11 | SNF1-related protein kinase catalytic subunit alpha KIN11, rabbit antibodiesAS09 613 | Anti-AKING1 | SNF1-related protein kinase regulatory subunit gamma 1, rabbit antibodiescollection of antibodies to proteins involved in signal transductionPlant protein extraction buffer
Gutierrez-Beltran et al, (2021) Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis, EMBO J, 2021 Jul 21:e105043, doi: 10,15252/embj,2020105043, Epub ahead of print, PMID: 34287990,Sun et al, (2021), Kinase SnRK1,1 Regulates nitrate channel SLAH3 Engaged in Nitrate-Dependent Alleviation of Ammonium Toxicity, Plant Physiol, 2021 Feb 9:kiab057, doi: 10,1093/plphys/kiab057, Epub ahead of print, PMID: 33560419,Belda-Palazón et al, (2020) A dual function of SnRK2 kinases in the regulation of SnRK1 and plant growth, Nat Plants, 2020 Nov;6(11):1345-1353, doi: 10,1038/s41477-020-00778-w, Epub 2020 Oct 19, PMID: 33077877,Krasnoperova et al, (2019), Potential Involvement of KIN10 and KIN11 Catalytic Subunits of the SnRK1 Protein Kinase Complexes in the Regulation of Arabidopsis ?-Tubulin, Cytology and Genetics, Volume 53, Issue 5, pp 349–356, (immunolocalization)Blanco et al, (2019), Dual and dynamic intracellular localization of Arabidopsis thaliana SnRK1,1, J Exp Bot, 2019 Apr 15;70(8):2325-2338, doi: 10,1093/jxb/erz023,
AKIN11 (E,C,= 2,7,11,1) is a catalytic subunit of the putative trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants, Synonymes: AKIN alpha-1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
AS09 460 | Anti-AKINB1 | SNF1-related protein kinase regulatory subunit beta-1, rabbit antibodiesAS09 462 | Anti-AKINB2 | SNF1-related protein kinase regulatory subunit beta-2, rabbit antibodiesAS15 2859 | Anti-AKIN ß3 | SNF1-related protein kinase regulatory subunit beta-3, rabbit antibodiesAS09 463 | Anti-AKIN beta gamma, rabbit antibodiesAS10 919 | Anti-AKIN10 SNF1-related protein kinase catalytic subunit alpha KIN10, rabbit antibodiesAS09 613 | Anti-AKING1 | SNF1-related protein kinase regulatory subunit gamma 1, rabbit antibodiescollection of antibodies to proteins involved in signal transductionPlant protein extraction buffer
Molecular Weight:
58,6 kDa
Not reactive in:
Oryza sativa, Vitis vinfera
Selected references:
Gutierrez-Beltran et al, (2021) Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis, EMBO J, 2021 Jul 21:e105043, doi: 10,15252/embj,2020105043, Epub ahead of print, PMID: 34287990,Pedrotti et al, (2018), Snf1-RELATED KINASE1-Controlled C/S1-bZIP Signaling Activates Alternative Mitochondrial Metabolic Pathways to Ensure Plant Survival in Extended Darkness, Plant Cell, 2018 Feb;30(2):495-509, doi: 10,1105/tpc,17,00414, Epub 2018 Jan 18,Emanuelle et al, (2015), SnRK1 from Arabidopsis thaliana is an atypical AMPK, Plant J, 2015 Mar 3, doi: 10,1111/tpj,12813,
AKIN beta-1 (AKINB1) is a member of SnRK1/SNF1/AMPK family in plants, It is a regulatory subunit of the putative trimeric SNF1-related protein kinase (SnRK) comples which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana AKIN beta-1 Q84VQ1, At5g21170
AS09 462 | Anti-AKINB2 | SNF1-related protein kinase regulatory subunit beta-2, rabbit antibodiesAS15 2859 | Anti-AKIN ß3 | SNF1-related protein kinase regulatory subunit beta-3, rabbit antibodiesAS09 463 | Anti-AKIN beta gamma, rabbit antibodiesAS10 919 | Anti-AKIN10 SNF1-related protein kinase catalytic subunit alpha KIN10, rabbit antibodiesAS10 920 | Anti-AKIN11 | SNF1-related protein kinase catalytic subunit alpha KIN11, rabbit antibodiesAS09 613 | Anti-AKING1 | SNF1-related protein kinase regulatory subunit gamma 1, rabbit antibodiescollection of antibodies to proteins involved in signal transductionPlant protein extraction bufferSecondary antibodies
Molecular Weight:
30,7 | 35 kDa
Not reactive in:
Zea mays
Selected references:
Belda-Palazón et al, (2020) A dual function of SnRK2 kinases in the regulation of SnRK1 and plant growth, Nat Plants, 2020 Nov;6(11):1345-1353, doi: 10,1038/s41477-020-00778-w, Epub 2020 Oct 19, PMID: 33077877,Crozet et al, (2016), SUMOylation represses SnRK1 signaling in Arabidopsis, Plant J, 2016 Jan;85(1):120-133, doi: 10,1111/tpj,13096,Emanuelle et al, (2015), SnRK1 from Arabidopsis thaliana is an atypical AMPK, Plant J, 2015 Mar 3, doi: 10,1111/tpj,12813,
AKIN beta-2 (AKINB2) is a member of SnRK1/SNF1/AMPK family in plants, It is a regulatory subunit of the putative trimeric SNF1-related protein kinase (SnRK) comples which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana AKIN beta-2 Q9SCY5
AKIN ß3 (SNF1-related protein kinase regulatory subunit beta-3) is a regulatory subunit of the probable trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brasica rapa, Capsella rubella, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us
AKING1 is a member of SnRK1/SNF1/AMPK family in plants, It is a regulatory subunit of the putative trimeric SNF1-related protein kinase (SnRK) comples which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana AKING1UniProt: Q8LBB2, TAIR: At3g48530
Emanuelle et al, (2015), SnRK1 from Arabidopsis thaliana is an atypical AMPK, Plant J, 2015 Mar 3, doi: 10,1111/tpj,12813,Ramon et al, (2013), The hybrid Four-CBS-Domain KIN?γ-subunit functions as the canonical γsubunit of the plant energy sensor SnRK1, Plant j, April 1,
AKIN beta gamma is a regulatory subunit of SNF1-related protein kinase (SnRK) complex, Involved in signal transduction cascade in carbohydrate metabolism, Alternative names: ATSNF4, homolog of yeast sucrose nonfermenting 4, KIN??, SNF4,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
This antibody is recognizing well recombinant AKIN beta gamma as well as the over-expressed protein in protoplasts, It has not been confirmed working on endogenous cell extract,
AKT1 is a highly selective inward-rectifying potassium channel located in cell membrane, that mediates potassium uptake by plant roots in response to low potassium conditions,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana AKT1 Q38998 , At2g26650
In the work of Honsbein et al, 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning, ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems, In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell),
Application Details:
1 : 50 with 125I (WB)
Purity:
Affinity purified serum in PBS pH 7,4
Reconstitution:
For reconstitution add 100 µl of sterile water,
Related products:
AS09 515 | Anti-KC1 | potassium channel KAT3, rabbit antibodiescollection of antibodies to membrane transport systemPlant protein extraction bufferSecondary antibodies
Molecular Weight:
96,9 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Safiarian et al, (2015), Lost in traffic? The K+ channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves and lily pollen, Front, Plant Sci, | doi: 10,3389/fpls,2015,00047,Honsbein et al, (2009), A tripartite SNARE-K+ channel complex mediates in channel-dependent K+ nutrition in Arabidopsis, The Plant Cell 21:2859-2877,
Special application note:
For detection images please, refer to the publication belowAntibody detects native and recombinant AKT1,
Fructose-1,6 bisphosphate aldolase (ALD) is an enzyme catalazying a key reaction of glycolysis and energy production, converting D-fructose- 1,6-bisphospate into dihydroxyacetone phosphate and D-glyceraldehyde-3-phosphate, This enzyme is present in plant and animal tissues, Plant enzyme is a class I aldolase which does not require a bivalent metal cofactor, It is located to outer mitochondrial membrane,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Glycne max, Oryza sativa, Picea sitchensis, Physcomitrella patens, Pisum sativum, Populus jackii, Spinacia oleracea, Vitis vinifera, Zea mays Species of your interest not listed? Contact us
Immunogen:
overexpressed cytosolic fructose 1,6 bisphosphate aldolase (ALD) based on the sequence from Arabidopsis thaliana Q9LF98, At3g52930
This product can be sold containing ProClin if requested,
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water,
Related products:
AS10 748 | Anti-ADH/ALDH | Alcohol/acetaldehyde dehydrogenase (bacterial/algal), rabbit antibodiesAS13 2731 | Anti-FBA | Fructose-bisphosphate aldolase class 2, rabbit antibodiesAS16 4093 | Anti- FBA | Fructose-bisphosphate aldolase (chloroplastic), rabbit antibodiescollection of antibodies to enzymes involved in carbohydrate metabolism
Molecular Weight:
38 | 38 kDa
Not reactive in:
Synechocystis sp,
Selected references:
Wang et al, (2018), iTRAQ-based quantitative proteomics analysis of an immature high-oleic acid near-isogenic line of rapeseed, Molecular Breeding January 2018, 38:2,Kamies et al, (2017), A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef, Proteomes, 2017 Nov 15;5(4), pii: E32, doi: 10,3390/proteomes5040032,Foley et al, (2017), A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate,Dev Cell, 2017 Apr 24;41(2):204-220,e5, doi: 10,1016/j,devcel,2017,03,018,Parveen et al, (2016), Chickpea Ferritin CaFer1 Participates in Oxidative Stress Response, and Promotes Growth and Development, Sci Rep, 2016 Aug 9;6:31218, doi: 10,1038/srep31218,Yam et al, (2016), Characterization of the Plasmodium Interspersed Repeats (PIR) proteins of Plasmodium chabaudi indicates functional diversity, Sci Rep, 2016 Mar 21;6:23449, doi: 10,1038/srep23449,Dixit (2015), Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves, Sci Rep, 2015 Nov 10;5:16205, doi: 10,1038/srep16205,Vera-Estrella et al, (2014), Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea, J Proteomics, 2014 Jun 2, pii: S1874-3919(14)00288-7, doi: 10,1016/j,jprot,2014,05,018,
Aleu protein may play a role in proteolysis leading to mobilization of nitrogen during senescence and starvation, Catalytic activity : Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-|-Xaa bonds) as well as an endopeptidase, Cellular localisation: vacuole, Alternative names: Senescence-associated gene product 2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml in PBS, 50% glycerol, Filter sterilized, No preservative or carrier added,
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica oleracea, Camelina sativa, Capsella rubella, Eutrema salsugineum, Raphanus sativus Species of your interest not listed? Contact us, tech@nktscientific.com
Immunogen:
Recombinant, His6-tagged, ALEU protein from Arabidopsis thaliana, UniProt: Q8H166, TAIR: At5g60360
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Takagi et al, et al, (2013), MAIGO5 functions in protein export from Golgi-associated endoplasmic reticulum exit sites in Arabidopsis, Plant Cell, 2013 Nov;25(11):4658-75, doi: 10,1105/tpc,113,118158, (Western blot, Arabidopsis thaliana)Ueda et al, (2006), AtVAM3 is required for normal specification of idioblasts, myrosin cells, Plant Cell Physiol, 2006 Jan;47(1):164-75, doi: 10,1093/pcp/pci232, (Western blot, Arabidopsis thaliana)
Alpha-mannosidase cleaves the alpha form of mannose,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in phosphate buffer saline
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Canavalia ensiformis (common jack-bean)
Immunogen:
native alpha-mannosidase purified from jack beans
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography,
Application Details:
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG
Reconstitution:
For reconstitution add 1 ml of sterile destilled water,
Related products:
AS09 453 | Anti-alpha-mannosidase (biotinylated), rabbit antibodiesAS09 650 | Anti-alpha-mannosidase (affinity purified), rabbit antibodiesPlant and algal protein extraction bufferSecondary antibodies
Not reactive in:
Arabidopsis thaliana
Special application note:
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition,
ANN-1 | Annexin-1 displays peroxidase activity and may counteract oxidative stress, Alternative names: ANNAT1, ANX23-ATH, ATOXY5, OXY5, Annexin D1, AnnAt1,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana Annexin-1, UniProt Q9SYT0, TAIR AT1G35720
Eukaryotic Translation Initiation Factor 4A-III (EIF4A3) belongs to the Asp-Glu-Ala-Asp (DEAD) box superfamily of proteins that have ATP-dependent RNA helicase activity and are involved in a number of areas of RNA biology, from transcription and translation to mRNA decay. EIF4A3 is named as such because it has homology at the amino-acid level to the translation initiation factors, EIF4A1 and EIF4A2, however it is actually a core component of the exon junction complex (EJC), which is associated with splicing and does not play an essential role in translation initiation (in contrast to EIF4A1 and EIF4A2).
EIF4A3 may have a role in parkinsons disease in relation to the deregulation of mRNA translation.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
100 µg in 100 µl PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Eukaryotic Translation Initiation Factor 4A-III (EIF4A3) belongs to the Asp-Glu-Ala-Asp (DEAD) box superfamily of proteins that have ATP-dependent RNA helicase activity and are involved in a number of areas of RNA biology, from transcription and translation to mRNA decay. EIF4A3 is named as such because it has homology at the amino-acid level to the translation initiation factors, EIF4A1 and EIF4A2, however it is actually a core component of the exon junction complex (EJC), which is associated with splicing and does not play an essential role in translation initiation (in contrast to EIF4A1 and EIF4A2).
EIF4A3 may have a role in parkinsons disease in relation to the deregulation of mRNA translation.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
50 µg in 50 µl PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
WNK1 is a cytoplasmic serine-threonine kinase involved in the regulation of electrolyte homeostasis, cell signalling, survival, and proliferation. It is part of the ERK5 MAP kinase pathway and acts as an activator of sodium-coupled chloride cotransporters such as SCNN1A, SCNN1B, SCNN1D and SGK1 and as an inhibitor of WNK4 by phosphorylation or through an interaction with the WNK1 autoinhibitory domain. WNK1 may also play a role in actin cytoskeletal reorganisation.
Defects in WNK1 are a cause of Gordon hyperkalemia-hypertension syndrome (pseudohypoaldosteronism type II - PHAII) an autosomal dominant disease characterised by severe hypertension and sensory and autonomic neuropathy type 2A (HSAN2A), a form of congenital sensory and autonomic neuropathy.
Homologs of this WNK1 have been found in Arabidopsis thaliana, C. elegans, Chlamydomonas reinhardtii and Vitis vinifera as well as in vertebrates including Danio rerio and Taeniopygia guttata.
This antibody was validated with HEK 293T cell lysate and HeLa cell lysate (1 in 1000 in 5% BSA)
Product Type:
NS Reagents Antibody
Antibody Type:
Monoclonal
Format:
100 µg in 100 µl PBS containing 0.02% sodium azide.
Mouse , Rat, Chicken, Duck, Hamster, Goat, Sheep, Cow, Dog, Cat, Pig and Horse.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
Immunogen:
A synthetic peptide from the C-terminal region of Human WNK1 - Ovalbumin Conjugated (The selected sequence is highly conserved across many species).
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Clone number:
Clone M42-P3B10
Antibody Isotype:
IgG1 kappa
Application Details:
WB 1:1000 in 5% BSA, IHC 1:50-1:500
Category:
Primary Antibodies
Other names:
WNK1, Erythrocyte 65 kDa protein, Human Accelerated Region 5, WNK Lysine Deficient Protein Kinase 1, hWNK1
Research Areas:
Cell Signalling, Neuroscience
NS Reagents Product Area:
Cell Signalling
Molecular Weight:
65kDa (Intended as a general guide and does not allow for all isoforms and species variations)
WNK1 is a cytoplasmic serine-threonine kinase involved in the regulation of electrolyte homeostasis, cell signalling, survival, and proliferation. It is part of the ERK5 MAP kinase pathway and acts as an activator of sodium-coupled chloride cotransporters such as SCNN1A, SCNN1B, SCNN1D and SGK1 and as an inhibitor of WNK4 by phosphorylation or through an interaction with the WNK1 autoinhibitory domain. WNK1 may also play a role in actin cytoskeletal reorganisation.
Defects in WNK1 are a cause of Gordon hyperkalemia-hypertension syndrome (pseudohypoaldosteronism type II - PHAII) an autosomal dominant disease characterised by severe hypertension and sensory and autonomic neuropathy type 2A (HSAN2A), a form of congenital sensory and autonomic neuropathy.
Homologs of this WNK1 have been found in Arabidopsis thaliana, C. elegans, Chlamydomonas reinhardtii and Vitis vinifera as well as in vertebrates including Danio rerio and Taeniopygia guttata.
This antibody was validated with HEK 293T cell lysate and HeLa cell lysate (1 in 1000 in 5% BSA)
Mouse , Rat, Chicken, Duck, Hamster, Goat, Sheep, Cow, Dog, Cat, Pig and Horse.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
Immunogen:
A synthetic peptide from the C-terminal region of Human WNK1 - Ovalbumin Conjugated (The selected sequence is highly conserved across many species).
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Clone number:
Clone M42-P3B10
Antibody Isotype:
IgG1 kappa
Application Details:
WB 1:1000 in 5% BSA, IHC 1:50-1:500
Category:
Primary Antibodies
Other names:
WNK1, Erythrocyte 65 kDa protein, Human Accelerated Region 5, WNK Lysine Deficient Protein Kinase 1, hWNK1
Research Areas:
Cell Signalling, Neuroscience
NS Reagents Product Area:
Cell Signalling
Molecular Weight:
65kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants, They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
According to Konert et al, (2015) AOX antibody is recognizing AOX1A and AOX1D,This product can be sold containing ProClin if requested,
Application Details:
1 : 750 (IL), 1 : 1000 for 10-20 µg of mitochondrial protein/lane detection (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water,
Related products:
AS06 152 Anti-AOX1 | alternative oxidase from Chlamydomonas reinhardtii, rabbit antibodiesAS04 054PRE | AOX1/2 | plant alternative oxidase 1 and 2, pre-immune serumAS04 054S | AOX | AOX positive control/quantitation standardPlant protein extraction bufferSecondary antibodies
Molecular Weight:
36-40 | 36-40 for Arabidopsis thaliana
Not reactive in:
Candidia albicans, Chlamydomonas reinhardtii (use an antibody to algal AOX1, AS06 152)
Selected references:
Pascual et al (2021), ACONITASE 3 is part of the ANAC017 transcription factor-dependent mitochondrial dysfunction response, Plant Physiology, 2021;, kiab225, https://doi,org/10,1093/plphys/kiab225Makino et al, (2020), Induction of Terminal Oxidases of Electron Transport Chain in Broccoli Heads Under Controlled Atmosphere Storage, Foods, 9 (4) Marchetti et al, (2020), Mitochondrial Pentatricopeptide Repeat Protein, EMB2794, Plays a Pivotal Role in NADH Dehydrogenase Subunit nad2 mRNA Maturation in Arabidopsis Thaliana, Plant Cell Physiol DOI: 10,1093/pcp/pcaa028 Garmash et al, (2020), Altered levels of AOX1a expression result in changes in metabolic pathways in Arabidopsis thaliana plants acclimated to low dose rates of ultraviolet B radiation, Plant Sci, 2020 Feb;291:110332, doi: 10,1016/j,plantsci,2019,110332,Kuang et al, (2019), Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins, Front Plant Sci, 2019 May 15;10:641, doi: 10,3389/fpls,2019,00641, eCollection 2019,
Special application note:
Mitochondrion inner membrane marker, Possibly in the inner surface of the inner mitochondrial membrane,Protocol for a plant mitochondria preparation can be found here, In protein samples which are older than few months AOX enzyme can undergo intensive dimerization, Such preparations should not be used to work with this antibody,
AP2 (floral homeotic protein APETALA 2) is a transcription factor involved in the specification of floral organ identity, establishment of floral meristem identity and controlling seed mass,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
This antibody was used in western blots on Lepidium appelianum and Lepidium campestre AP2 Protein expressed in E, coli cells, Applied dilution was 1: 6000,Contains 0,02 % sodium azide,
Wang et al, (2021), Brassinosteroids inhibit miRNA-mediated translational repression by decreasing AGO1 on the endoplasmic reticulum, J Integr Plant Biol, 2021 May 21, doi: 10,1111/jipb,13139, Epub ahead of print, PMID: 34020507,
APX plays a key role in plant antioxidant system by reducing hydrogen peroxide to water, Cellular localization includes chloroplast (tAPX and sAPX), cytosol (cAPX) and peroxisome (pAPX),
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
BSA-conjugated synthetic peptide derived from Arabidopsis thaliana tAPX (thylakoidal ascorbate peroxidase) UniProt: Q42593-1, TAIR:At1g77490 and sAPX (stromal/mitochondrial ascorbate peroxidase) UniProt: Q42592-1 TAIR: At4g08390 Five out of twelve amino acids are also identical with cAPX1 (At1g07890), cPX2 (At3g09640) and pAPX (At4g35000)
This product can be sold containing proclin if requested
Application Details:
1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water,
Related products:
AS06 180 | Anti-cAPX, rabbit antibodies
Molecular Weight:
25-38 kDa for A, thaliana
Not reactive in:
Algae, Helianthus annus, Marchantia polymorpha
Selected references:
Jedelská et al, (2021) Protein S-nitrosation differentially modulates tomato responses to infection by hemi-biotrophic oomycetes of Phytophthora spp, Hortic Res, 2021 Feb 1;8(1):34, doi: 10,1038/s41438-021-00469-3, PMID: 33518717; PMCID: PMC7848004,Tokarz et al, (2021), Stem Photosynthesis-A Key Element of Grass Pea (Lathyrus sativus L,) Acclimatisation to Salinity, Int J Mol Sci, 2021 Jan 12;22(2):685, doi: 10,3390/ijms22020685, PMID: 33445673; PMCID: PMC7828162,Tokarz et al, (2020), Can Ceylon Leadwort ( Plumbago zeylanica L,) Acclimate to Lead Toxicity?-Studies of Photosynthetic Apparatus Efficiency, Int J Mol Sci, 2020 Mar 9;21(5):1866,doi: 10,3390/ijms21051866, Molnár et al, (2020), Nitro-oxidative Signalling Induced by Chemically Synthetized Zinc Oxide Nanoparticles (ZnO NPs) in Brassica Species, Chemosphere, 251, 126419 Jedelská et al, (2019), Tomato Root Growth Inhibition by Salinity and Cadmium Is Mediated By S-Nitrosative Modifications of ROS Metabolic Enzymes Controlled by S-Nitrosoglutathione Reductase, Biomolecules, 2019 Aug 21;9(9), pii: E393, doi: 10,3390/biom9090393,
Arabinogalactans are polymers composed of arabinose and galactose monosaccharides, They exist in plants as free glycans or are attached to rhamnogalacturonan I or to protetein backbones, When attached to proteins they form arabinogalactan protein (AGP) which works as an intercellular signaling molecule, AGP also functions as a glue for sealing wounds in plants, Arabinogalactans can be used as an additive in food, and as a replacement for starch in food or pharmaceutical products,
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Cell culture supernatant, liquid
Storage Temp:
Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year, Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes,
Arabinogalactans are polymers composed of arabinose and galactose monosaccharides, They exist in plants as free glycans or are attached to rhamnogalacturonan I or to protetein backbones, When attached to proteins they form arabinogalactan protein (AGP) which works as an intercellular signaling molecule, AGP also functions as a glue for sealing wounds in plants, Arabinogalactans can be used as an additive in food, and as a replacement for starch in food or pharmaceutical products, This pectic polysaccharide antibody belongs to the arabinogalactan 3 group of antibodies, Pectin consists of polysaccharides found in the primary cell wall of most plants which binds cells together in the middle lamella,
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Cell culture supernatant, liquid
Storage Temp:
Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year, Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes,
Arabinogalactans are polymers composed of arabinose and galactose monosaccharides, They exist in plants as free glycans or are attached to rhamnogalacturonan I or to protetein backbones, When attached to proteins they form arabinogalactan protein (AGP) which works as an intercellular signaling molecule, AGP also functions as a glue for sealing wounds in plants, Arabinogalactans can be used as an additive in food, and as a replacement for starch in food or pharmaceutical products, This pectic polysaccharide antibody belongs to the arabinogalactan-4 group of antibodies, Pectin consists of polysaccharides found in the primary cell wall of most plants which binds cells together in the middle lamella,
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Cell culture supernatant, liquid
Storage Temp:
Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year, Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes,
ARC5 (Dynamin-like protein ARC5) is a probable GTPase component of both plastid and peroxisme division machinery, Required for the last steps of plastid division specifically in mesophyll-cell, when the narrow isthmus breaks, facilitating the separation of the daughter plastids, Necessary for peroxisome activities, Seems to influence stromule (stroma-filled tubular extensions of the plastid envelope membrane) length and frequency, Alternative names: Dynamin-related protein 5B Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in Tris 0,1M, glycine 0,1M, sucrose 2%
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Shelf life upon re-constitution is 6 months, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes, Store reconstituted antibodies at 4°C or in -20° for extrended periods of time,Lyophilized powder is stable for a minimum of 2 years at -20°C,
Host Animal:
Rabbit
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from internal domain of Dynamin-like protein ARC5 from Arabidopsis thaliana Uniprot: Q84N64,TAIR: AT3G19720
Antibody format is a total IgG (purified on Protein A),
Application Details:
1 : 500-1 : 5 000 (WB)
Purity:
Total IgG
Reconstitution:
For reconstitution add 50 µl of sterile water,
Related products:
collection of antibodies to proteins involved in photosynthesisPlant protein extraction buffer Secondary antibodies
Molecular Weight:
87 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Pipitone et al, (2021), A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis, Elife, 2021 Feb 25;10:e62709, doi: 10,7554/eLife,62709, PMID: 33629953; PMCID: PMC7906606,
The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport, It is a small GTPase that undergoes a GDP/GTP nucleotide exchange cycle and it is an important regulator of cellular trafficking,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
References describing immunolocalization (IF) and (IG) studies:Pimpl et al (2000), In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles, Plant Cell, 2000 Nov;12(11):2219-36,Ritzenthaler et al, (2002), Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera, Plant Cell, 2002 Jan;14(1):237-61,
Application Details:
1 : 1000 (IF), 1 : 100 (IG), 1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water,
Related products:
AS08 327 | Anti-Sec21 (gamma subunit, COP vesicles)(Golgi marker in immunolocalization and COP1 marker in western blot), rabbit antibodiesAS08 325PRE | Arf1 | ADP-ribosylation factor 1, pre-immune serumrecommended secondary antibodyPlant and algal protein extraction buffer Secondary antibodies
Molecular Weight:
21 kDa (Arabidopsis thaliana)
Not reactive in:
Microsporidia sp,
Selected references:
Narasimhan et al, (2021) Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking, Plant Physiol, 2021 Mar 18:kiab134, doi: 10,1093/plphys/kiab134, Epub ahead of print, PMID: 33734402,Hurny et al, (2020), SYNERGISTIC ON AUXIN AND CYTOKININ 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance, Nat Commun, 2020 May 1;11(1):2170, doi: 10,1038/s41467-020-15895-5, (immunolocalization)Kuang et al, (2019), Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins, Front Plant Sci, 2019 May 15;10:641, doi: 10,3389/fpls,2019,00641, eCollection 2019,Singh et al, (2018), A single class of ARF GTPase activated by several pathway-specific ARF-GEFs regulates essential membrane traffic in Arabidopsis, PLoS Genet, 2018 Nov 15;14(11):e1007795, doi: 10,1371/journal,pgen,1007795,Gonzaga Heredia-Martinez et al, (2018), Chloroplast damage induced by the inhibition of fatty acid synthesis triggers autophagy in Chlamydomonas, Plant Physiol, Sept, 2018,
Special application note:
Cellular [compartment marker] of Golgi in immunolocalization and COP1 in western blot
Actin-related proteins (ARPs) are found in the nuclei of all eukaryotic cells, but their functions are generally understood only in the context of their presence in various yeast and animal chromatin-modifying complexes, Arabidopsis thaliana ARP6 is a clear homolog of other eukaryotic ARP6s, including Saccharomyces cerevisiae ARP6, which was identified as a component of the SWR1 chromatin remodeling complex, Arabidopsis ARP6 is localized to the nucleus during interphase but dispersed away from the chromosomes during cell division, ARP6 expression was observed in all vegetative tissues as well as in a subset of reproductive tissues, Null mutations in ARP6 caused numerous defects, including altered development of the leaf, inflorescence, and flower as well as reduced female fertility and early flowering in both long- and short-day photoperiods,
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Cell culture supernatant
Storage Temp:
Store at -80°C; Avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes,
Host Animal:
Mouse
Species Reactivity:
Arabidopsis thaliana
Immunogen:
ARP6 of Arabidopsis thaliana, UniProt: Q8LGE3, TAIR: At3g33520
Applications:
ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
collection of antibodies to proteins involved in regulation of transcription
Molecular Weight:
47 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Deal et al, (2005), The nuclear actin-related protein ARP6 is a pleiotropic developmental regulator required for the maintenance of FLOWERING LOCUS C expression and repression of flowering in Arabidopsis, Plant Cell Oct;17(10):2633-46, doi:10,1105/tpc,105,035196,
Actin-related proteins (ARPs) are found in the nuclei of all eukaryotic cells, but their functions are generally understood only in the context of their presence in various yeast and animal chromatin-modifying complexes, Arabidopsis ARP8 shows 30 and 29% amino acid identity to yeast actin and Arabidopsis ACT2 in the regions of alignment, respectively, Because it is not closely related to yeast or human ARP8 and shows similar weak homology to yeast ARP8 and ARP9, the Arabidopsis ARP8 is considered a plant-specific orphan ARP,
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Cell culture supernatant
Storage Temp:
Store at -80°C; Avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes,
Host Animal:
Mouse
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us, tech@nktscientific.com
Antibody is recognizing following epitope, amino acids 447-471: SNLSIFPGPWCITRKQFRRKSRLMWThis antibody is recognizing the full-length 52 kDa recombinant ARP8 protein expressed in Escherichia coli as well as endogenous ARP8 of identical molecular weight in Arabidopsis thaliana extracts from different tissues: all vegetative and reproductive organs examined including seedlings, roots and siliques, with higher concentrations of the protein detected in developing flower buds and flowers within the inflorescence,
Clone number:
IgG1
Application Details:
assay dependent
Related products:
collection of antibodies to proteins involved in regulation of transcription
Molecular Weight:
52 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Kandasamy et al, (2008), ACTIN-RELATED PROTEIN8 encodes an F-box protein localized to the nucleolus in Arabidopsis, Plant Cell Physiol, 49(5):858-63, doi: 10,1093/pcp/pcn053,
Actin-related proteins (ARPs) are found in the nuclei of all eukaryotic cells, but their functions are generally understood only in the context of their presence in various yeast and animal chromatin-modifying complexes, Arabidopsis ARP8 shows 30 and 29% amino acid identity to yeast actin and Arabidopsis ACT2 in the regions of alignment, respectively, Because it is not closely related to yeast or human ARP8 and shows similar weak homology to yeast ARP8 and ARP9, the Arabidopsis ARP8 is considered a plant-specific orphan ARP,
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Cell culture supernatant
Storage Temp:
Store at -80°C; Avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes,
Host Animal:
Mouse
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica rapa, Camelina sativa, Capsella rubella, Eutrema salsugineum, Raphanus sativusSpecies of your interest not listed? Contact us, tech@nktscientific.com
Antibody is recognizing following epitope, amino acids 2-26: aa 2-ILKKVWG SVWNRSNSGKDLVNHQRA-26 This antibody is recognizing the full-length 52 kDa recombinant ARP8 protein expressed in Escherichia coli as well as endogenous ARP8 of identical molecular weight in Arabidopsis thaliana extracts from different tissues: all vegetative and reproductive organs examined including seedlings, roots and siliques, with higher concentrations of the protein detected in developing flower buds and flowers within the inflorescence,
Clone number:
IgG1
Application Details:
assay dependent
Related products:
collection of antibodies to proteins involved in regulation of transcription
Molecular Weight:
52 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Kandasamy et al, (2008), ACTIN-RELATED PROTEIN8 encodes an F-box protein localized to the nucleolus in Arabidopsis, Plant Cell Physiol, 49(5):858-63, doi: 10,1093/pcp/pcn053,
ASN (Glutamine-dependent asparagine synthetase) is essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem, ASN2 is expressed in leaf and ASN1 is expressed in floral organs, The amino acid sequences of Arabidopsis thaliana ASN1 and ASN2 are 76% identical, The amino acid sequences of Arabidopsis thaliana and Zea mays ASN2 are 73,6% identical,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml in PBS, 50% glycerol, Filter sterilized, No preservative or carrier added,
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
Brassica rapa, Camelina sativa, Capsella rubella, Eutrema salsugineum, Punica granatumSpecies of your interest not listed? Contact us, tech@nktscientific.com
Immunogen:
Purified full length, tag cleaved, recombinant Arabidopsis thaliana ASN2, UniProt: Q9LV77 , TAIR: AT5G65010
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
collection of antibodies to proteins involved in nitrogen metabolism
Molecular Weight:
65 | 65 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Gaufichon et al, (2017), ASN1-encoded asparagine synthetase in floral organs contributes to nitrogen filling in Arabidopsis seeds, Plant J, 2017 Aug;91(3):371-393, doi: 10,1111/tpj,13567,Gaufichon et al, (2013), Arabidopsis thaliana ASN2 encoding asparagine synthetase is involved in the control of nitrogen assimilation and export during vegetative growth, Plant Cell Environ, 2013 Feb;36(2):328-42, doi: 10,1111/j,1365-3040,2012,02576,x,
Special application note:
This antibody reacts with both isoforms: ASN1 and ASN2,
Pairing and synapsis of homologous chromosomes is required for normal chromosome segregation and the exchange of genetic material via recombination during meiosis, Synapsis is complete at pachytene following the formation of a tri-partite proteinaceous structure known as the synaptonemal complex (SC), In yeast, HOP1 is essential for formation of the SC, and localises along chromosome axes during prophase I, Homologues in Arabidopsis (AtASY1), Brassica (BoASY1) and rice (OsPAIR2) have been isolated through analysis of mutants that display decreased fertility due to severely reduced synapsis of homologous chromosomes, Analysis of these genes has indicated that they play a similar role to HOP1 in pairing and formation of the SC through localisation to axial/lateral elements of the SC,
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Mouse
Species Reactivity:
Triticum aestivum
Expected Species:
Triticum aestivum
Immunogen:
KLH-conjugated synthetic pepitde chosen from Triticum aestivum ASY1 UniProt: A7TVU8
This antibody does not work in immunolocalization,
Application Details:
1: 10 000 (ELISA), 1: 1000 (WB)
Purity:
Affinity purified
Reconstitution:
For reconstitution add 50 µl of sterile water/tube,
Related products:
antibodies to proteins involved in DNA/RNA/Cell cycle
Molecular Weight:
66,3 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Darrier et al, (2019), Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High-Resolution Microscopy, Methods Mol Biol, 2020;2061:207-215, doi: 10,1007/978-1-4939-9818-0_15,
At2g21960 is an integral component of thylakoid membrane,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Higher plants Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide, derived from Arabidopsis thaliana, UniProt: Q9SJ03, TAIR: AT2G21960
ADP ribosylation factor 2 is a GTP-binding protein which is involved in protein trafficking, ArfAlternative name: ARF1-like protein U5
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
synthetic peptide derived from Arabidopsis thaliana Q9SRC3
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel,Diluted antibody solution can be used 2 to 3 times within one month if it contains 0,1 % sodium azide as preservative and is stored at -20ºC to -80ºC,
Application Details:
1 : 8000 (ELISA), 1 : 2000 (WB)
Purity:
Serum
Related products:
collection of antibodies to plant endomembrane transport system Plant protein extraction bufferSecondary antibodies
Molecular Weight:
20,5 | 29 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Special application note:
0,1 % sodium azide is added as preservative, For antibody re-suspending information check the tube lable,Antibodies will detect target protein in a few µg of a crude preparation loaded per well, If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient,
CCaP1 acts as a mediator in response to continuous dark or gibberellic acid, Alternative name: At1g62480/T3P18_4, T3P18,4
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes,
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel,Diluted antibody solution can be used 2 to 3 times within one month if it contains 0,1 % sodium azide as preservative and is stored at -20ºC to -80ºC,
Application Details:
1 : 8000 (ELISA), 1 : 1000 (WB)
Purity:
Serum
Related products:
collection of antibodies to tonoplast proteins Plant protein extraction bufferSecondary antibodies
Molecular Weight:
16,6 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Ide et al, (2007), Transcriptional Induction of Two Genes for CCaPs, Novel Cytosolic Proteins, in Arabidopsis thaliana in the Dark, Plant Cell Physiol, 1:54-65,
Special application note:
0,1 % sodium azide is added as preservative, For antibody re-suspending information check the tube lable,Antibodies will detect target protein in a few µg of a crude preparation loaded per well, If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient,
ATG12b (Autophagy-related protein 12b) is an Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt) and autophagy vesicles formation, Alternative names: Ubiquitin-like protein ATG12B, APG12-like protein b, AtAPG12b,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
Recombinant, full length ATG12b ofArabidopsis thaliana protein sequence UniProt: Q9LVK3-1, TAIR: At3g13970
10,4 | 12 and 50 kDa in wilde type plants and 12 kDa in some autophagy deficient mutants
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Chung et al, (2010), ATG8 lipidation and ATG8-mediated autophagy in Arabidopsis require ATG12 expressed from the differentially controlled ATG12A AND ATG12B loci, Plant J, 2010 May;62(3):483-93, doi: 10,1111/j,1365-313X,2010,04166,x,
UniProt number:
Q9LVK3-1
TAIR number:
At3g13970
Research Area:
Protein Modifications/Autophagy-related and Ubiquitin-like
ATG13a (Autophagy-related protein 13a) is involved in autophagy in a nutritional condition dependent manner, The ATG1-ATG13 protein kinase complex regulates downstream events required for autophagosome enclosure and/or vacuolar delivery, Alternative names: AtAPG13a,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Noccaea caerulescens Species of your interest not listed? Contact us
Immunogen:
Full length, recombinant ATG13a of Arabidopsis thaliana protein sequence UniProt: Q9SCK0, TAIR: At3g49590 with N-terminal HIS-tag,
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Suttangkakul et al, (2011), The ATG1/ATG13 protein kinase complex is both a regulator and a target of autophagic recycling in Arabidopsis, Plant Cell, 2011 Oct;23(10):3761-79, doi: 10,1105/tpc,111,090993,
ATG16 (Autophagy-related protein 16) may play a role in autophagy process,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
N-terminal end of of ATG16 of Arabidopsis thaliana with the PB1 and ZZ domain, UniProt: Q6NNP0-1, TAIR: At5g50230
ATG1a (Serine/threonine-protein kinase ATG1a) is a serine/threonine protein kinase involved in autophagy in a nutritional condition-dependent manner, The ATG1-ATG13 protein kinase complex regulates downstream events required for autophagosome enclosure and/or vacuolar delivery, Becomes a target of autophagy under nutrient starvation and connects autophagy to plant nutritional status, Alternative names: Autophagy-related protein 1a, AtAPG1a,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Noccaea caerulescens Species of your interest not listed? Contact us
Immunogen:
Full length, N-terminally HIS-tagged ATG1a of Arabidopsis thaliana protein sequence UniProt: Q94C95-1, TAIR: At3g61960, overexpressed in E,coli and injected as a gel piece
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Suttangkakul et al, (2011), The ATG1/ATG13 protein kinase complex is both a regulator and a target of autophagic recycling in Arabidopsis, Plant Cell, 2011 Oct;23(10):3761-79, doi: 10,1105/tpc,111,090993,
ATG3 (Autophagy-related protein 3) is a E2 conjugating enzyme responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8, This step is required for the membrane association of ATG8, Alternative names: Autophagy-related E2-like conjugation enzyme ATG3, AtAPG3, Protein autophagy 3,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica campestris, Eutrema salsugineum, Helianthus annuus, Nicotiana tabacum, Noccaea caerulescens, Populus trichocarpa, Salvia splendens, Sisymbrium irio, Solanum tuberosum Species of your interest not listed? Contact us
Immunogen:
Full length recombinant ATG3 of Arabidopsis thaliana protein sequence UniProt: Q0WWQ1-1, TAIR: At5g61500, overexpressed in E,coli
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Phillips et al, (2008), The ATG12-conjugating enzyme ATG10 Is essential for autophagic vesicle formation in Arabidopsis thaliana, Genetics, 2008 Mar;178(3):1339-53, doi: 10,1534/genetics,107,086199,
ATG4 (Autophagy protein 4) is a protein with cyteine-type endopeptidase activity involved in autophagy process,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Coccomyxa subellipsoidea C-169, Tetraselmis sp, GSL018, Volvox carteri Species of your interest not listed? Contact us
Immunogen:
recombiant ATG4 of Chlamydomonas reinhardtii, Gene ID Cre12,g510100
Technical details how to work with this antibody are provided here: Pérez-Pérez et al, (2016), Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease, Plant Physiol, 2016 Dec;172(4):2219-2234,
Chen et al, (2016), The role of nitric oxide signalling in response to salt stress in Chlamydomonas reinhardtii, Planta, 2016 Sep;244(3):651-69, doi: 10,1007/s00425-016-2528-0, Epub 2016 Apr 26,Pérez-Pérez et al, (2016), Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease, Plant Physiol, 2016 Dec;172(4):2219-2234,
Special application note:
This antibody is recognizing 25 ng of recombinant CrATG4This product can be sold containing ProClin if requested,
ATG5 (Autophagy protein 5) is a protein required for autophagy, Involved in a negative feedback loop that modulates NPR1-dependent salicylic acid (SA) signaling and limits senescence and immunity-related programmed cell death (PCD) in plants and a complete proteolysis of chloroplast stroma proteins in senescent leaves, in the degradation of damaged peroxisomes, Alternative names: Protein autophagy 5, AtAPG5,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Solanum lycopersicum, Solanum tuberosum
Immunogen:
Full length, recombinant ATG5 of Arabidopsis thaliana protein sequence UniProt: Q9FFI2-1, TAIR: At5g17290, overexpressed in E,coli
38,5 | 50 kDa (in wilde type plants) and ca, 38 kDa in autophagy defective mutants
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Thompson et al, (2005), Autophagic nutrient recycling in Arabidopsis directed by the ATG8 and ATG12 conjugation pathways, Plant Physiol, 2005 Aug;138(4):2097-110,
FUNCTION: Required for autophagy, Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome, The conjugate detaches from the membrane immediately before or after autophagosome formation is completed, May play an important role in the apoptotic process, possibly within the modified cytoskeleton, Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent, Glycerol (1:1) may be added for an additional stability, Avoid repetitive freeze/thaw cycles,
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to the C-terminal of human ATG-5L, No immunogenic carrier protein was conjugated to the immunogen, Instead, Adjukine B has been used to enhance the immune response,
Applications:
Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
ATG5 (Autophagy-related protein 5) the protein forms a conjugate with ATG12 with an essential role in plant nutrient recycling, It is involved in complete proteolysis of chloroplast stroma proteins in senescent leaves and degradation of damaged peroxisomes,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Recombinant ATG5 of Arabidopsis thaliana
Immunogen:
Recombinant ATG5 of Arabidopsis thaliana, UniProt:Q9FFI2, TAIR:AT5G17290
AS14 2811 | Anti-ATG8A | Autophagy-related protein 8a, rabbit antibodiesAS12 1854 | Anti-NPR1 | Nonexpresser of PR genes 1 (Arabidopsis thaliana), rabbit antibodiesAS16 4108 | Anti-NPR1 | Nonexpresser of PR genes 1 (other species)Collection of antibodies to plant proteasomePlant and algal protein extraction bufferSecondary antibodies
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Young et al, (2019), A facile forward-genetic screen for Arabidopsis autophagy mutants reveals twenty-one loss-of-function mutations disrupting six ATG genes, Autophagy, 2019 Jun;15(6):941-959, doi: 10,1080/15548627,2019,1569915,
Special application note:
This antibody is so far recognizing recombinant ATG5 from Arabidopsis thaliana and reactivity on endogenous protein needs to be confrimed, It does not react with 6xHis-ATG7,This product can be sold containing ProClin if requested,
ATG7 (Autophagy-related protein 7) is an E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy, Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine, Acts in the senescence process, degradation of damaged peroxisomes and non-selective degradation of chlorophylls and photosynthetic proteins during stress-induced leaf yellowing,Alternative names: Ubiquitin-like modifier-activating enzyme atg7, ATG12-activating enzyme E1 atg7, AtAPG7, Protein PEROXISOME UNUSUAL POSITIONING 4,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica rapa, Capsella rubella , Citrus clementina, Noccaea caerulescens Species of your interest not listed? Contact us
Immunogen:
Recombinant ATG7 of Arabidopsis thaliana, UniProt: Q94CD5-1, TAIR: At5g45900, overexpressed in E,coli, collected in inclusion bodies, which were solubilized before immunization
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Doelling et al, (2002), The APG8/12-activating enzyme APG7 is required for proper nutrient recycling and senescence in Arabidopsis thaliana, J Biol Chem, 2002 Sep 6;277(36):33105-14,
Special application note:
Protein name was changed from APG7 to ATG7 after Doelling paper was published,
UniProt number:
Q94CD5-1
TAIR number:
At5g45900
Research Area:
Protein Modifications/Autophagy-related and Ubiquitin-like
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Actinidia chinensis var, chinensis, Cajanus cajan, Capsicum annuum, Corchorus olitorius, Cucumis melo, Glycine max, Glycine soja, Gossypium arboreum, Juglans regia, Malus domestica, Nelumbo nucifera, Nicotiana tabacum, Nicotiana benthamiana, Nicotiana sylvestris, Noccaea caerulescens, Prunus yedoensis var, nudiflora, Solanum chacoense, Solanum lycopersicum, Vigna radiataSpecies of your interest not listed? Contact us, tech@nktscientific.com
Immunogen:
Recombinant Arabidopsis thaliana ATG7 UniProt Q94CD5, TAIR AT5G45900
ATG8 (Autophagy-related protein) involved in degradation and recycling of intracellular components in a process of autophagy,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Recombinant ATG8 A-I of Arabidopsis thaliana
Immunogen:
Part of recombinant ATG8A from Arabidopsis thaliana, UniProt:Q8LEM4,TAIR:AT4G21980
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Fan et al, (2019), Dual Role for Autophagy in Lipid Metabolism in Arabidopsis, Plant Cell, 2019 Jul;31(7):1598-1613, doi: 10,1105/tpc,19,00170, (Immunogold)Dong et al, (2017), Sulfur availability regulates plant growth via glucose-TOR signaling, Nat Commun, 2017 Oct 27;8(1):1174, doi: 10,1038/s41467-017-01224-w,
Special application note:
This product can be sold containing ProClin if requested,
ATG8 (Autophagy-related protein 8) is involved in degradation and recycling of intracellular components in a process of autophagy, ATG8 is a molecular autophagy marker in Chlamydomonas reinhardtii (Pérez-Pérez et al, 2010, Plant Physiol, 152: 1874-88),
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
For Arabidopsis thaliana the signal obtained using ATG8 antibodies is cleaner in case of roots compare to leaf material, For best results please follow extraction protocol described in Álvarez et al, (2012), ATG8 signal corresponds to the two bands of 17 kDa,Preparation of a cell extract from Arabidopsis thaliana:A, Plants were first subjected to autophagy activating conditions: nutrient (nitrogen or carbon) limitation or oxidative stress in order to activate this degradative process,B, Total protein extracts can be obtained as described by Álvarez, Leaves are grinded in liquid nitrogen with a minimal volume of extraction buffer (100 mM Tris-HCl pH 8, 400 mM sucrose, 1 mM EDTA, 0,1 mM phenylmethylsulfonyl fluoride (PMSF), 10 mg/ml sodium deoxycholate, 10 µg/ml of leupeptin, 10 µg/ml of pepstatin A, 4% (v/v) protease inhibitor cocktail from Roche),C, Cell debris is removed by centrifuging at 500 g for 10 min at 4°C,Important note:It is recommendable to use bigger gels in order to get a better resolution of ATG8 bands, Midi-protean gels are better than mini-gels, There are 9 ATG8 isoforms and this antibody will likely recognizes all of them,For immunolocalization protocol, please inquire,
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Li et al, (2021) The unfolded protein response plays dual roles in rice stripe virus infection through fine-tuning the movement protein accumulation, PLoS Pathog, 2021 Mar 4;17(3):e1009370, doi: 10,1371/journal,ppat,1009370, Epub ahead of print, PMID: 33662041,Li et al, (2021) Chloroplast proteotoxic stress-induced autophagy is involved in the degradation of chloroplast proteins in Chlamydomonas reinhardtii, Plant Cell Physiol, 2021 Feb 17:pcab029, doi: 10,1093/pcp/pcab029, Epub ahead of print, PMID: 33594417,Kazibwe et al, (2020), TOR mediates the autophagy response to altered nucleotide homeostasis in a ribonuclease mutant, J Exp Bot, 2020 Sep 9;eraa410,doi: 10,1093/jxb/eraa410,Upadhyaya and Jagadeeshwar Rao (2019), Reciprocal regulation of photosynthesis and mitochondrial respiration by TOR kinase in Chlamydomonas reinhardtii, Plant Direct Volume 3, Issue 11,Shull et al, (2019), Anatase TiO2 nanoparticles induce autophagy and chloroplast degradation in thale cress (Arabidopsis thaliana), Environ Sci Technol, 2019 Jul 29, doi: 10,1021/acs,est,9b01648,
Special application note:
This product can be sold containing ProClin if requested,This antibody is recognizing 1 ng of recombinant CrATG8, Antigen used to elicit this antibody is conserved from 70-80 % in following ATG protein from Arabidopsis thaliana: ATG8a UniProt: Q8LEM4 ATG8B UniProt: Q9XEB5 ATG8c UniProt: Q8S927 ATG8d UniProt: Q9SL04, ATG8e UniProt: Q8S926 ATG8f UniProt: Q8VYK7 and conserved below 70 % in: ATG8g UniProt: Q9LZZ9 ATG8h Uniprot: Q8S92This antibody does not recognize all isoforms into the same degree,
ATG9 (Autophagy-related protein 9) is required for autophagy that plays an essential role in plant nutrient recycling, Alternative name: AtAPG9,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Plant ATG9 is most likely N-glycosylated, and glycosylated membrane proteins can migrate as more than one form in SDS-PAGE (smear); the protein most likely forms functional dimers which could be detected also after western blotting, Usage of microsomal fraction is highly recommended,
ATG9 (Autophagy-related protein 9) is required for autophagy that plays an essential role in plant nutrient recycling, Alternative name: AtAPG9,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica campestris, Brassica rapa subsp, pekinensis, Capsella rubella, Eutrema salsugineum Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana ATG9 protein sequence, N-terminal part, UniProt: Q8RUS5, TAIR: AT2G31260
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient, AtpA is the largest subunit of the membrane-extrinsic ATP synthase subcomplex,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Dicots and (including Lilium superbum) chloroplast AtpA; may cross-react with mitochondrial AtpA; Algae, Nannochloropsis gaditana, CyanobacteriaSpecies of your interest not listed? Contact us, tech@nktscientific.com
Immunogen:
Recombinant maize chloroplast AtpA P05022
Applications:
Immunogold (IG), Immunoprecipitation (IP), Western blot (WB)
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Pao et al, (2018), Lamelloplasts and minichloroplasts in Begoniaceae: iridescence and photosynthetic functioning, J Plant Res, 2018 Mar 2, doi: 10,1007/s10265-018-1020-2, (ImmunoGold)Zhang et al, (2017), Nitric oxide induces monosaccharide accumulation through enzyme S-nitrosylation, Plant Cell Environ, 2017 Sep;40(9):1834-1848, doi: 10,1111/pce,12989,Jeon et al, (2017), Functional characterization of chloroplast-targeted RbgA GTPase in higher plants, Plant Mol Biol, 2017 Nov;95(4-5):463-479, doi: 10,1007/s11103-017-0664-y,Murcha et al, (2016), Plant specific Preprotein and Amino Acid Transporter proteins are required for tRNA import into mitochondria, Plant Physiol, 2016 Oct 27, pii: pp,01519,2016,Camejo et al, (2015), Proteomic identification of mitochondrial carbonylated proteins in two maturation stages of pepper fruits, Proteomics, 2015 Aug;15(15):2634-42, doi: 10,1002/pmic,201400370,Yang et al, (2015), Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum, Protein Expr Purif, 2015 May 12, pii: S1046-5928(15)00111-4, doi: 10,1016/j,pep,2015,05,006,
Special application note:
Sequence of the protein used for eliciting this antibody is also conserved in Arabidopsis thaliana AtpA P56757
AAA2 domain is present in ATP binding proteins involved in stress response and being members of clpA/clpB family,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 23,3 µg/µl in PBS pH 8,0 + 0,02% sodium azide as preservative
Storage Temp:
Store at 2-8°C; make aliquots to avoid working with a stock, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes,
Acinetobacter baumannii, Chlamydomonas reinhardtii, , Clostridium sp,, Cyanobacteria, Glycine max, Nannochloropsis gaditana, Oryza sativa, marine Diatoms, Vitis vinifera, Yersinia sp, Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3
Applications:
Immunolocalization (IL), Immunogold (IG), Western blot (WB)
Levitan et al, (2019), Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum, Proc Natl Acad Sci U S A, 2019 Aug 27;116(35):17316-17322, doi: 10,1073/pnas,1906726116,Nelson et al, (2019), Protein lysine methylation contributes to modulating the response of sensitive and tolerant Arabidopsis species to cadmium stress, doi: 10,1111/pce,13692,Gellért et al, (2018), A single point mutation on the cucumber mosaic virus surface induces an unexpected and strong interaction with the F1 complex of the ATP synthase in Nicotiana clevelandii plants, Virus Res, 2018 Jun 2;251:47-55, doi: 10,1016/j,virusres,2018,05,005, (immunogold)Quesada et al, (2011), Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development, Plant J, Nov;68(4):738-53, doi: 10,1111/j,1365-313X,2011,04726,x, Epub 2011 Sep 13,Andersson et, al (2009), Co-localization of P-glycerate kinase, P-ribulokinase, ADP-glucose pyrophosphorylase and Rubisco activase with CF1 in pea leaf chloroplasts, Plant Science 177:136-14, (immunologold)Morash et al, (2007), Macromolecular dynamics of the photosynthetic system over a seasonal developmental progression in Spartina alterniflora, Canadian J, of Botany, 2007, 85(5): 476-483, 10,1139/B07-043,
Special application note:
The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 ATP synthase and most known bacterial F-type ATP synthases, Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein,
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Acinetobacter baumannii, Algae, Cannabis sativa, Clostridium sp,, Diatoms, Cyanobacteria, E,coli K-12, Vitis vinifera, Yersinia sp, Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1 At5g08670 as well as Chlamydomonas reinhardtii P06541 and A8IQU3
Applications:
Blue Native-PAGE (BN-PAGE), Immunofluorescence (IF), Western blot (WB)
Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well, Gel thickness was 2 mm with 4,5-16 % gradient,Antibody is recognizing mitochondrial form of AtpB Subota el, al (2011),This antibody can be used as a loading control for bacteria, Bacillus cereus,
Cecchin et al (2021) LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii, Plant J, 2021 Jul 4, doi: 10,1111/tpj,15405, Epub ahead of print, PMID: 34218480,Aihara et al, (2019), Algal photoprotection is regulated by the E3 ligase CUL4-DDB1DET1, Nat Plants, 2019 Jan;5(1):34-40, doi: 10,1038/s41477-018-0332-5,Jespersen et al, (2017), Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass, Front Plant Sci, 2017 Jul 11;8:1224, doi: 10,3389/fpls,2017,01224, eCollection 2017, (western blot, Agostis stolonifera cv, ‘Penncross’)Rurek et al, (2015), Biogenesis of mitochondria in cauliflower (Brassica oleracea var, botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure, Biochim Biophys Acta, 2015 Jan 21, pii: S0005-2728(15)00016-X, doi: 10,1016/j,bbabio,2015,01,005,Eom et al, (2014), Bacillus subtilis HJ18-4 from Traditional Fermented Soybean Food Inhibits Bacillus cereus Growth and Toxin-Related Genes, J Food Sci, 2014 Nov;79(11):M2279-87, doi: 10,1111/1750-3841,12569, Epub 2014 Oct 30,
Special application note:
This product can be sold containing proClin if requested,The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 Atp Synthase, and most known bacterial F-type Atp Synthases, Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein,
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta UniProt: P19366, TAIR: AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1, UniProt: P83483, TAIR: At5g08670 as well as Chlamydomonas reinhardtii, UniProt: P06541 and A8IQU3
Applications:
Blue Native-PAGE (BN-PAGE), Immunofluorescence (IF), Western blot (WB)
Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well, Gel thickness was 2 mm with 4,5-16 % gradient,Antibody is recognizing mitochondrial form of AtpB Subota el, al (2011),This antibody can be used as a loading control for bacteria, Bacillus cereus,
Chen, Liu & Liu (2021) Loss-Function of EGY1 Results in Photosynthesis Damage through Reducing Stability of Photosystem II in Arabidopsis thaliana, Russ J Plant Physiol (2021), https://doi,org/10,1134/S1021443721060029Curien et al, (2021) Mixotrophic growth of the extremophile galdieria sulphuraria reveals the flexibility of its carbon assimilation metabolism, New Phytol, 2021 Mar 25, doi: 10,1111/nph,17359, Epub ahead of print, PMID: 33764540,Maclean et al, (2021) Complexome profile of Toxoplasma gondii mitochondria identifies divergent subunits of respiratory chain complexes including new subunits of cytochrome bc1 complex, PLoS Pathog, 2021 Mar 2;17(3):e1009301, doi: 10,1371/journal,ppat,1009301, PMID: 33651838,Picariello et al, (2020), TIM, a Targeted Insertional Mutagenesis Method Utilizing CRISPR/Cas9 in Chlamydomonas Reinhardtii, PLoS One, 2020 May 13;15(5):e0232594, doi: 10,1371/journal,pone,0232594,Pattanaik et al, (2020), Introduction of a green algal squalene synthase enhances squalene accumulation in a strain of Synechocystis sp, PCC 6803, Metabolic Engineering Communications,Volume 10, June 2020, e00125
Special application note:
The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 Atp Synthase, and most known bacterial F-type Atp Synthases, Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein,Anti-AtpB antibody was used as a loading control in Chlamydomonas reinhardtii and Synechocystis sp, PCC6803,This product can be sold containing proclin if requested
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient, This enzyme is localised in mitochondrial inner membrane,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Brassica oleracea var, botrytis cv, 'Diadom'
Expected Species:
Chlamydomonas reinhardtii, Nicotiana tabacum, Phaeodactylum tricornutum Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from available plant, algal mitochondrial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana ATP synthase subunit beta-1 UniProt:P83483, TAIR:AT5G08670ATP synthases subunit beta-2 UniProt:P83484, TAIR: AT5G08690, ATPase subunit beta-3, UniProt: Q9C5A9, TAIR:AT5G08680, which belong to mitochondrial respiratory chain complex I,
AS05 085 | Anti-AtpB (chloroplastic+mitochondrial isoforms) rabbit antibodiesAS03 030 | Anti-AtpB (chloroplastic+mitochondrial isoforms) chicken antibodies (developed to exactly the same peptide as AS05 085)AS03 030S | ATP synthase subunit beta protein standard, for quantitation and positive control for AS05 085Collection of antibodies to mitochondrial proteinsrecommended secondary antibodyPlant and algal protein extraction buffer Secondary antibodies
Molecular Weight:
59,6 | 55 kDa
Selected references:
Wei et al, (2019), Arabidopsis mtHSC70-1 plays important roles in the establishment of COX-dependent respiration and redox homeostasis, J Exp Bot, 2019 Aug 6, pii: erz357, doi: 10,1093/jxb/erz357,
Special application note:
Lack of antibody reactivity was confirmed on chloroplast fraction,This product can be sold containing ProClin if requested,
AtPCaP1 is a protein associated with plasma membrane with ability to bind Ca, calmodulin and phosphatidylinositol phoshphates, Alternative names: At4g20260, endomembrane-associated protein,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Raphanus sativus (41 kDa), Brassica rapa (42 kDa), Brassica rapa var, glabra Regel (43 kDa), Brassica oleracea var, italica (41 kDa)
Expected Species:
Brassicaceae Species of your interest not listed? Contact us
It is confirmed that the nock-out mutant (SALK_022955, Col-0 background) has no mRNA or protein of PCaP1, In the immunoblot, crude membrane fractions prepared from wild-type and pcap1 knock-out mutant plants were examined, Therefore the antibody to PCaP1 specifically recognizes PCaP1 and can be used for immunoblotting, For application in immunocytochemistry cross reacting band in proximity of Rubisco has to be removed by incubation of an antibody with a part of memrane with background band, Protocol for preparation of crude membranes This protocol will help to prepare crude membrane fractions from very small amount of plant tissue, (1) Homogenize roots or root tips with the buffer without SDS,(2) Centrifuge the homogenate in Eppendorf tubes at 2 000 rpm for 2 min to remove cell wall, nuclei, plastids and mitochondria, (3) Centrifuge the obtained supernatant at 30 000 g for 20 min to separate soluble components, Please use adequate tubes for ultracentrifugation, (4) Suspend the obtained precipitate (crude membranes) with SDS buffer with DTT or beta-mercaptoethanol, (5) Heat the sample solution obtained the step (4) at 70 C for 5 min, (6) Apply the denatured sample to SDS-PAGE,Courtesy Prof, Msayoshi Maeshima, Nagoya University, Japan
Application Details:
1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water,
Related products:
antibodies to plasma membrane proteinsPlant protein extraction buffer Secondary antibodies
Molecular Weight:
24,5 | 36 kDa
Not reactive in:
Nicotiana benthamiana
Selected references:
Nagasaki et al, (2008) A hydrophilic cation-binding protein of Arabidopsis thaliana, AtPCaP1, is localized to plasma membrane via N-myristoylation and interacts with calmodulin and the posphatidylinositol phosphates PtdIns(3,4,5)P3 and PtdIns(3,5)P2, FEBS J, 275: 2267-2282,Ide et al, (2007) Molecular properties of a novel, hydrophilic cation-binding protein associated with the plasma membrane, J, Exp, Botany, 58: 1173-1183,
Special application note:
Serum contains less than 0,1 % of sodium azide as preservative,
ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane, F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel, CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1), CF(0) has three main subunits: a, b and c, The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex, Alternative name of gamma subunit is also: F-ATPase gamma subunit,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Apparent molecular weight of subunit gamma (and as general rule most of ATP synthase subunits) is quite different between Chlamydomonas (42 kDa) and higher plants (38 kDa in spinach), see figure in Lemaire et al, (1989),
Pipitone et al, (2021), A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis, Elife, 2021 Feb 25;10:e62709, doi: 10,7554/eLife,62709, PMID: 33629953; PMCID: PMC7906606,Storti et al, (2020), The activity of chloroplast NADH dehydrogenase-like complex influences the photosynthetic activity of the moss Physcomitrella patens, doi,org/10,1101/2020,01,29,924597Pralon et al, (2019), Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency, Commun Biol, 2019 Jun 20;2:220, doi: 10,1038/s42003-019-0477-4,Li et al, (2019), A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis, Nat Genet, 2019 Apr;51(4):627-635, doi: 10,1038/s41588-019-0370-6,Liang et al, (2018), Thylakoid-Bound Polysomes and a Dynamin-Related Protein, FZL, Mediate Critical Stages of the Linear Chloroplast Biogenesis Program in Greening Arabidopsis Cotyledons, Plant Cell, 2018 Jul;30(7):1476-1495, doi: 10,1105/tpc,17,00972, Epub 2018 Jun 7,
Special application note:
This product can be sold containing ProClin if requested,
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria, ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at at Store short-term 4°C, Long-term -20°, Repeated freezing and thawing is not recommended,
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Blair et al, (2018), The Helicobacter pylori cell shape promoting protein Csd5 interacts with the cell wall, MurF, and the bacterial cytoskeleton, Mol Microbiol, 2018 Jul 24, doi: 10,1111/mmi,14087,Fristedt et al, (2015), The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana, PLoS One, 2015 Apr 2;10(4):e0121658, doi: 10,1371/journal,pone,0121658,
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria, ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4°C, Long-term -20°, Repeated freezing and thawing is not recommended, Solution contains 0,01% sodium azide as preservative,
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Galvis et al, (2020), H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in chloroplast ATP synthase mutants, Plant Physiol, pp,01561,2019, doi: 10,1104/pp,19,01561,Koochak et al, (2019), The structural and functional domains of plant thylakoid membranes, Plant J, 2019 Feb;97(3):412-429, doi: 10,1111/tpj,14127,Lv et al, (2019), Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant, Plant Cell, 2019 Jan;31(1):210-230, doi: 10,1105/tpc,18,00813,Gao et al, (2018), A supercomplex, approximately 720 kDa and composed of both photosystem reaction centers, dissipates excess energy by PSI in green macroalgae under salt stress, Plant Cell Physiol, 2018 Oct 8, doi: 10,1093/pcp/pcy201,Koochak et al, (2018), The structural and functional domains of plant thylakoid membranes, Plant J, 2018 Oct 12, doi: 10,1111/tpj,14127, (BN-PAGE)Rantala and Tikkanen et al, (2018), Phosphorylation‐induced lateral rearrangements of thylakoid protein complexes upon light acclimation, Plant Direct Vol, 2, Issue 2,Fristedt et al, (2015), The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana, PLoS One, 2015 Apr 2;10(4):e0121658, doi: 10,1371/journal,pone,0121658, Grieco et al, (2015), Light-harvesting II antenna trimers connect energetically the entire photosynthetic machinery - including both photosystems II and I, Biochim Biophys Acta, 2015 Jun-Jul;1847(6-7):607-19, doi: 10,1016/j,bbabio,2015,03,004, Epub 2015 Apr 3,Yap at al, (2015), AEF1/MPR25 is implicated in RNA editing of plastid atpF and mitochondrial nad5 and also promotes atpF splicing in Arabidopsis and rice, Plant J, 2015 Jan 13, doi: 10,1111/tpj,12756,
Special application note:
This product can be sold containing proClin if requested,
ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane, F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel, CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1), CF(0) has three main subunits: a, b and c,Alternative names: ATPase subunit II, ATP synthase F(0) sector subunit b'
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Chlamydomonas reinhardtii
Expected Species:
Algae, Oryza sativa, Sorghum bicolor, Zea mays, Volvox carteri Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated mix of synthetic peptide derived from Arabidopsis thaliana AtpG Q0WMW8, At4g32260 and Chlamydomonas reinhardtii ATP synthase subunit b' A8J785
F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient, Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Algae, Cannabis sativa, Glycine max, Oryza sativa, Physcomitrella patens, Pisum sativum, Populus alba, Pinus thunbergii, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
Purified c subunit from Spinacia oleracea UniProt: P69447,
Note that increased incubation at 95ºC (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e,g, 5 or 6 subunits) may occur,
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Nath et al, (2016), A Nitrogen-Fixing Subunit Essential for Accumulating 4Fe-4S-Containing Photosystem I Core Proteins, Plant Physiol, 2016 Dec;172(4):2459-2470, Epub 2016 Oct 26,Lawrence et al, (2010), Recombinant production and purification of the subunit c of chloroplast ATP synthase, Protein Expression and Purification 76: 15-24,
Special application note:
This product can be sold containing proclin if requested,
F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient, Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Chlamydomonas reinhardtii
Expected Species:
Algae, Cannabis sativa, Glycine max, Hordeum vulgare, Oryza sativa, Ostreococcus tauri, Physcomitrella patens, Pinus thunbergii, Pisum sativum, Populus alba, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptides derived from AtpH subunit c of Arabidopsis thaliana UniProt: P56760, TAIR: AtCg00140 and Chlamydomonas reinhardtii UniProt: Q37304
Please note that increased incubation at 95ºC (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e,g, 5 or 6 subunits) may occur,
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Schulz et al, (2017), Molecular architecture of the N-type ATPase rotor ring from Burkholderia pseudomallei, EMBO Rep, 2017 Apr;18(4):526-535, doi: 10,15252/embr,201643374,
Special application note:
This product can be sold containing ProClin if requested,
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria, ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient, The transmembrane CF0IV subunit of appr, 25 kDa belongs to a stator part of ATP synthase and is involved in the proton translocation,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4°C, Long-term -20°, Repeated freezing and thawing is not recommended, Solution contains 0,01% sodium azide as preservative,
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Fristedt et al, (2015), The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana, PLoS One, 2015 Apr 2;10(4):e0121658, doi: 10,1371/journal,pone,0121658,
AUX1 (Auxin transporter protein 1) is a carrier protein involved in proton-driven auxin influx, Synthesized in developing leaves and transpported to tips, Involved in lateral root formation, trichoblast polarization and root hair elongation, Required for gravitropism and thigmotropism, especially in roots, by modulating responses to auxin, ethylene and cytokinins, Needed for ammonium-mediated root-growth inhibition, Alternative names: AUX1, AUXIN RESISTANT 1, WAV5, WAVY ROOTS 5, PIR1, MAP1, MODIFIER OF ARF7/NPH4 PHENOTYPES 1, Auxin influx carrier protein 1, Polar auxin transport inhibitor-resistant protein 1,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Goat
Species Reactivity:
Arabidopsis thaliana (recombinant AUX1)
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana AUX1, UniProt: Q96247, TAIR: AT2G38120
AUX1 (Auxin transporter protein 1) is a carrier protein involved in proton-driven auxin influx, Synthesized in developing leaves and transpported to tips, Involved in lateral root formation, trichoblast polarization and root hair elongation, Required for gravitropism and thigmotropism, especially in roots, by modulating responses to auxin, ethylene and cytokinins, Needed for ammonium-mediated root-growth inhibition, Alternative names: AUX1, AUXIN RESISTANT 1, WAV5, WAVY ROOTS 5, PIR1, MAP1, MODIFIER OF ARF7/NPH4 PHENOTYPES 1, Auxin influx carrier protein 1, Polar auxin transport inhibitor-resistant protein 1,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana (recombinant AUX1)
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana AUX1, UniProt: Q96247, TAIR: AT2G38120
BAK1 (Brassinosteroid insensitive 1-associated receptor kinase 1) controls expression of genes associated with innate immunity in the absence of pathogens or elicitors, Phosphorylated Brl1, Involved in programmed cell death (PCD), Subcellular localization: cell membrane, Alternative names: BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1, BAK1, BRI1-ASSOCIATED RECEPTOR KINASE, RKS10, RECEPTOR KINASES LIKE SERK 10, SERK3, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3, ELG, ELONGATED, ATSERK3, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3, ATBAK1,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Solanum lycopersicum
Expected Species:
Thelungiella halophila Species of your interest not listed? Contact us
Extra Information on CE extraction buffer: CE buffer does not need to be made freshly everytime, Aliquots can be kept at -20°C, Na2MoO4 and NaF are phosphatase inhibitors, included to prevent lose phosphorylation form our protein of interest during extraction, EDTA chelates metal ions and thus inhibits many enzymes which need metal ions as co-factors and inhibits the action of proteases, Protease inhibitor coctail is Sigma product number P 9599 which is used in dilution 1: 100,Antibody is reconizing AtBAK1-Myc in total extracts,
Katarzyna Parys et al (2021) Signatures of antagonistic pleiotropy in a bacterial flagellin epitope,Cell Host & Microbe,Volume 29, Issue 4,2021,Pages 620-634,e9,ISSN 1931-3128,https://doi,org/10,1016/j,chom,2021,02,008,(https://www,sciencedirect,com/science/article/pii/S1931312821000871)Zhang et al, (2019), An important role of L -fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity, New Phytol, 2018 Dec 15, doi: 10,1111/nph,15639,Hu et al, (2018), A group of receptor kinases are essential for CLAVATA signalling to maintain stem cell homeostasis, Nat Plants, 2018 Apr;4(4):205-211, doi: 10,1038/s41477-018-0123-z, (CoIP)Bundy et al, (2016), A mutation in the catalytic subunit of the glycosylphosphatidyl inositol transamidase disrupts growth, fertility and stomata forma formation in Arabidopsis, Plant Physiology, DOI:10,1104/pp,16,00339,Tateda et al, (2014), Salicylic Acid Regulates Arabidopsis Microbial Pattern Receptor Kinase Levels and Signaling,Plant Cell, 2014 Oct 14, pii: tpc,114,131938,
Special application note:
Antibodies bind to AtBAK1-Myc, They do not cross react with AtSOBIR,This product can be sold containing proclin if requested,
Beta amylase (EC 3,2,1,2) is an enzyme involved in the hydrolysis fo starch into sugars, Can play a minor role in the starch degradation and maltose metabolism in chloroplasts during the night,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
The mature length protein of Arabidopsis thaliana BAM1 overexpressed in E,coli, UniProt:Q9LIR6, TAIR: AT3G23920,, lacking the transit peptide that is cleaved upon entry to the chloroplast, Recombinant protein had an N-terminal S-tag,
Beta amylase (EC 3,2,1,2,) catalyzes the hydrolysis of the second alfa-1,4 glycosidic bond, Alternative names 1,4-alfa-D-glucan maltohydrolase, glycogenase,saccharogen, amylase),
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS labelled with biotin
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Kappaphycus alvarezii, Solanum tuberosum
Expected Species:
Arabidopsis thaliana, Glycine max, Physcomitrella patens, Populus trichocarpa, Ricinus communis, Vitis viniferaSpecies of your interest not listed? Contact us, tech@nktscientific.com
Immunogen:
Beta amylase isolated and purified from sweet potato UniProt:Q94EU9
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition,
Application Details:
1 : 1000-1 : 4000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG
Reconstitution:
For reconstitution add 1 ml of sterile water,
Related products:
AS09 379 | Anti-beta amylase antibodiesPlant and algal protein extraction bufferSecondary antibodies
Molecular Weight:
60 kDa
Selected references:
Usuldin et al, (2017), Molecular investigation of carrageenan production in Kappaphycus alvarezii in different culture conditions: a proteomic approach, ournal of Applied Phycology, August 2017, Volume 29, Issue 4, pp 1989–2001, (Kappaphycus alvarezii)
Special application note:
Biotin/IgG protein molar ratio (B/P) is approximately 6,6, No foreign proteins are added, Marker used for lebling is N-hydroxysuccinimidoBiotin,
BetaCA1 (Beta carbonic anhydrase 1) (chloroplastic) - is a zinc metalloenzyme that interconvert CO2 and HCO3 (-), Alternative names: ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3, ATBCA1, ATSABP3, BETA CARBONIC ANHYDRASE 1, CA1, CARBONIC ANHYDRASE 1, SABP3, SALICYLIC ACID-BINDING PROTEIN 3,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Solanum lycopersicum Q5NE20 Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide, derived in the part C-terminus of BetaCA1 of Arabidopsis thaliana, UniProt: P27140, TAIR: At3g01500
Extraction method – Grind 50 mg of leaf tissue in a sterile microcentrifuge tube using a sterile plastic pestle, Add 132µL of Protein Extraction Buffer (1x TE, 1,2 %SDS, 2,7% sucrose, 7,5 µg mL-1 bromophenol blue) to the ground leaf tissue, Vortex the sample and keep on ice for 15 mins, Centrifuge at 14,000 rpm for five minutes using a benchtop centrifuge, Collect the supernatant and in a new sterile 0,5 ml microcentrifuge tube and discard the pellet,This antibody does not recognize betaCA2,
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
DiMario et al, (2016), The Cytoplasmic Carbonic Anhydrases ?CA2 and ?CA4 Are Required for Optimal Plant Growth at Low CO2, Plant Physiol, 2016 May;171(1):280-93, doi: 10,1104/pp,15,01990,
BG1 (Beta-glucosidase 1) hydrolyzes abscisic acid glucose ester (ABA-GE) which represents the predominant form of conjugated ABA (biologically inactive), No activity with beta-D-glucopyranosyl zeatin, The hydrolysis of ABA-GE in the endoplasmic reticulum (ER) forms free ABA and contributes to increase its cellular levels under dehydration conditions, ABA-GE hydrolyzing activity is enhanced by dehydration stress-induced polymerization into higher molecular weight forms, The ABA produced by BGLU18 contributes to the initiation of intracellular signaling as well as the increase in the extracellular ABA level, Localised to ER lumen,Alternative names: AtBG1, Beta-glucosidase 18, Beta-glucosidase homolog 1,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml in PBS, 50% glycerol, Filter sterilized, No preservative or carrier added,
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us, tech@nktscientific.com
Immunogen:
Purified recombinant BG1 of Arabidopsis thaliana, residues 27-528 with a His6-thioredoxin tagged, UniProt: Q9SE50, TAIR: At1g52400
Applications:
Immunolocalisation (IL) using electron microscopy, Western blot (WB)
BIK1 (Botrytis-induced kinase 1) belongs to the protein kinase superfamily, BIK1 is a crucial component of host response signaling required to activate the resistance responses to Botrytis and A, brassicicola infection,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7,4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Noccaea caerulescensSpecies of your interest not listed? Contact us, tech@nktscientific.com
Ngou et al, (2021) Mutual potentiation of plant immunity by cell-surface and intracellular receptors, Nature, 2021 Mar 10, doi: 10,1038/s41586-021-03315-7, Epub ahead of print, PMID: 33692545,
Special application note:
This product can be sold containing ProClin if requested,
BIN2 (Brasinosteroid insensitive 2) plays an important role in regulating brassinosteroid signalling which is important for plant growth, It is also implied to mediate auxing signalling pathway, Phosphorylation of BZR1 and BZR2/BES2 by BIN2 increases degradation of these proteins by the proteasome,Alternative names:ATSK21, DWARF 12, DWF12, SHAGGY-LIKE KINASE 21, SK21, UCU1, ULTRACURVATA 1
Product Type:
Antibody
Antibody Type:
Polyclonal
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Biotin carboxyl carrier subunit of chloroplast ACCase is a biotin carboxyl-carrier subunit of the multi-enzyme plastidial acetyl-coenzyme A carboxylase complex involved in fatty acid biosynthesis process,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
For reconstitution add 100 µl of sterile water,Lyophilized antibody can be stored at -20 or -80°C, Once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER, BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER), Alternative name: AtBP2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Piea sitchensis, Populus trichocarpa, Spinacia oleracea, Zea mays Species of your interest not listed? Contact us
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel,
Application Details:
1 : 50-1 : 1000 (IF), 1 : 2000 (WB)
Purity:
Affinity purified IgY in PBS pH 7,4
Related products:
AS09 481 | Anti-BiP2 | lumenal-binding protein 2, rabbit antibodiesAS09 615 | Anti-BiP2 | lumenal-binding protein 2, goat antibodiesantibodies to plant endomembrane system proteins Plant protein extraction bufferSecondary antibodies
Molecular Weight:
73,5 | 80 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Bennett et al, (2014), Plasma Membrane-Targeted PIN Proteins Drive Shoot Development in a Moss, Curr Biol, 2014 Dec 1;24(23):2776-85, doi: 10,1016/j,cub,2014,09,054, Epub 2014 Nov 13,
Special application note:
Antibody solution contains 0,02% sodium azide as preservative,
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER, BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER), Alternative name: AtBP2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Picea sitchensis, Populus trichocarpa, Physcomitrella patens, Spinacia oleracea, Zea mays Species of your interest not listed? Contact us
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel,Antibody has a reduced reactivity to monocots in western blot,
Application Details:
1 : 2000 (WB)
Purity:
Affinity purified serum in PBS, pH 7,4
Reconstitution:
For reconstitution add 100 µl of sterile water,
Related products:
AS09 614 | Anti-BiP2 | lumenal-binding protein, 2 chicken antibodiesAS09 481 | Anti-BiP2 | lumenal-binding protein 2, rabbit antibodiesantibodies to plant endomembrane system proteins Plant protein extraction bufferSecondary antibodies
Molecular Weight:
73,5 | 80 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Narusaka et al (2016), Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1, Sci Rep, 2016 Jan 11;6:18702, doi: 10,1038/srep18702,
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER, BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER),
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid, conjugated to HRP
Storage Temp:
Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years,
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER, BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER),
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel, This antibody has so far not worked in IP,
Application Details:
1 : 8000 (ELISA), 1 : 600 (IF), 1 : 2000 (WB)
Purity:
Affinity purified serum in PBS, pH 7,4
Reconstitution:
For reconstitution add 50 µl of sterile water,
Related products:
AS09 615 | Anti-BiP2 | lumenal-binding protein 2, goat antibodiesAS09 614 | Anti-BiP2 | lumenal-binding protein 2, chicken antibodiesAS09 481PRE | BiP | lumenal-binding protein, pre-immune serumantibodies to plant endomembrane system proteins Plant protein extraction bufferSecondary antibodies
Molecular Weight:
73,5 | 80 kDa
Not reactive in:
Ostreococcus tauri
Selected references:
Gu et al, (2021) A Lipid Bodies-Associated Galactosyl Hydrolase Is Involved in Triacylglycerol Biosynthesis and Galactolipid Turnover in the Unicellular Green Alga Chlamydomonas reinhardtiiHurny et al, (2020), SYNERGISTIC ON AUXIN AND CYTOKININ 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance, Nat Commun, 2020 May 1;11(1):2170, doi: 10,1038/s41467-020-15895-5, (immunolocalization)Yang et al, (2020), PROTEIN PHOSPHATASE 95 Regulates Phosphate Homeostasis by Affecting Phosphate Transporter Trafficking in Rice, Plant Cell, 2020 Jan 9, pii: tpc,00685,2019, doi: 10,1105/tpc,19,00685,Jang et al, (2020), 1Molecules and CellsCrABCA2 Facilitates Triacylglycerol Accumulation in Chlamydomonas reinhardtii under Nitrogen Starvation, Mol Cells, 2020 Jan 31;43(1):48-57, doi: 10,14348/molcells,2019,0262,Mares et al, (2020), Hydrosoluble phylloplane components of Theobroma cacao modulate the metabolism of Moniliophthora perniciosa spores during germination,Fungal Biol, 2020 Jan;124(1):73-81, doi: 10,1016/j,funbio,2019,11,008,
Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages, This is a suitable tool for proximity labelling experiments as described in Mair et al, (2019), Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID, Elife , 2019 Sep 19;8:e47864, doi: 10,7554/eLife,47864,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
BirA (mutated/TurboID)
Immunogen:
Recombinant mutated BirA protein from E,coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177), Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag),
Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages, This is a suitable tool for proximity labelling experiments as described in Mair et al, (2019), Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID, Elife , 2019 Sep 19;8:e47864, doi: 10,7554/eLife,47864, This tag has MW of 35 kDa,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid, conjugated to ALP
Storage Temp:
Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years,
Host Animal:
Rabbit
Species Reactivity:
BirA (mutated/TurboID)
Immunogen:
Recombinant mutated BirA protein from E,coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177), Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag),
Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages, This is a suitable tool for proximity labelling experiments as described in Mair et al, (2019), Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID, Elife , 2019 Sep 19;8:e47864, doi: 10,7554/eLife,47864, This tag has MW of 35 kDa,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid, conjugated to biotin,
Storage Temp:
Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years,
Host Animal:
Rabbit
Species Reactivity:
BirA (mutated/TurboID)
Immunogen:
Recombinant mutated BirA protein from E,coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177), Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag),
Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages, This is a suitable tool for proximity labelling experiments as described in Mair et al, (2019), Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID, Elife , 2019 Sep 19;8:e47864, doi: 10,7554/eLife,47864, This tag has MW of 35 kDa,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid, conjugated to HRP
Storage Temp:
Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years,
Host Animal:
Rabbit
Species Reactivity:
BirA (mutated/TurboID)
Immunogen:
Recombinant mutated BirA protein from E,coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177), Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag),
BRI1 (Protein BRASSINOSTEROID INSENSITIVE 1) is a receptor which binds brassinolide and has a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates, Involved in a signaling cascade including expression of light- and stress-regulated genes, promotion of cell elongation, normal leaf and chloroplast senescence, and flowering, Alternative names:BRI1, BRASSINOSTEROID INSENSITIVE 1, CBB2, CABBAGE 2, DWF2, DWARF 2, BIN1, BR INSENSITIVE 1, ATBRI1, Brassinosteroid LRR receptor kinase
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7,4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus, Brassica rapaSpecies of your interest not listed? Contact us, tech@nktscientific.com
Antibody was tested on bri1-1 and bri1-5 mutants, Bri1-1 is a point mutation in the kinase domain that renders the protein non-functional and plants compensate for that by over-accumulating the non-functional receptor, Bri1-5 is a mutant in the extracellular domain and the bri1-5 protein is retained in the ER, The bri1-5 plants contain less protein than the wild type and show an intermediate brassinosteroid deficient phenotype, Also BRI1-5 migrates higher than wild type BRI1 in SDS-PAGE, because it carries ER-type high mannose N-glycans, For IP: 15 µl GFP-trap beads was used for 200 mg plant material to precipitate GFP-tagged protein followed by detection with Co-IPed BRI1 on Western with 1:5000 diluted anti-BRI1 antibody,Protein extraction has to be done efficiently as this step is crucial, recommended material to buffer ratio: 15 µl/µg or less,
Lee et al (2021), Chaperone-like protein DAY plays critical roles in photomorphogenesis, Nat Commun, 2021 Jul 7;12(1):4194, doi: 10,1038/s41467-021-24446-5, PMID: 34234144; PMCID: PMC8263706,Chen et al, (2019), BES1 is activated by EMS1-TPD1-SERK1/2-mediated signaling to control tapetum development in Arabidopsis thaliana, Nat Commun, 2019 Sep 13;10(1):4164, doi: 10,1038/s41467-019-12118-4,Hou et al, (2019), Less Conserved LRRs Is Important for BRI1 Folding, Front Plant Sci, 2019 May 21;10:634, doi: 10,3389/fpls,2019,00634, eCollection 2019,Chen et al, (2019), BZR1 Family Transcription Factors Function Redundantly and Indispensably in BR Signaling but Exhibit BRI1-Independent Function in Regulating Anther Development in Arabidopsis, Mol Plant, 2019 Jun 20, pii: S1674-2052(19)30207-2, doi: 10,1016/j,molp,2019,06,006,
Special application note:
This product can be sold containing proclin if requested,
The brassinosteroid (BR) signaling pathway is regulated by complex mechanisms which at the transcriptional level involves BRI1 negatively regulated by BZR1 and BES1, BZR1 mediates downstream brassinosteroid-regulated growth response and feedback inhibition of brassinosteroid biosynthetic genes,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
The antibody may be stored at -20°Cfor one year in its original formulation, Additionally, antibody may be stored at 2°C to 8°C for up to 1 month without detectable loss of activity, Avoid repeated freeze-thaw cycles of the diluted antibody,store at 4°C; make aliquots to avoid working with a stock, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Oryza sativa
Expected Species:
Hordeum vulgare Species of your interest not listed? Contact us
Ca2+-ATPase | calmodulin-stimulated calcium-ATPase belongs to family of cation transport ATPase (P-type),
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes,
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel,
Application Details:
1 : 8000 (ELISA), 1 : 1000 (WB)
Purity:
Serum
Related products:
collection of antibodies to tonoplast proteins Plant protein extraction bufferSecondary antibodies
Molecular Weight:
111 | 110 kDa (Raphanus sativus)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Siddiqui et al, (2020), Melatonin and calcium function synergistically to promote the resilience through ROS metabolism under arsenic-induced stress, Journal of Hazardous MaterialsVolume 398, 5 November 2020, 122882
Special application note:
0,1 % sodium azide is added as preservative, For antibody re-suspending information check the tube lable,This protein is of low abundance in plant tissues,
CAH3 - carbonic anhydrase is a zinc-containing metalloenzyme that catalyzes the reversible interconversion of CO2 and HCO3?, It plays an important role in many physiological functions that involve decarboxylation or carboxylation reactions, including both photosynthesis and respiration,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Chlamydomonas reinhardtii (only)
Immunogen:
Recombinant carbonic anhydrase of Chlamydomonas reinhardtii A8J4Z8 after cleavage of a fusion protein
Applications:
immunofluorescence (IF), Immunogold (IG), Western blot (WB)
AS11 1737 | Anti-Beta-CA1, beta-CA2 | carbonic anhydrase | mitochondrial | Chlamydomonas, rabbit antibodiesantibodies to other photosynthetic proteinsCollection of antibodies to Chlamydomonas proteinsAlgal protein extraction buffer Secondary antibodies
Molecular Weight:
33,4 | 29 kDa
Not reactive in:
Arabidopsis thaliana, Oryza sativa
Selected references:
Shukshina &Terentyev (2021) Involvement of Carbonic Anhydrase CAH3 in the Structural and Functional Stabilization of the Water-Oxidizing Complex of Photosystem II from Chlamydomonas reinhardtii, Biochemistry Moscow 86, 867–877 (2021), https://doi,org/10,1134/S0006297921070075Terentyev (2020: The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated From Wild Type and cia3 Mutant Chlamydomonas reinhardtii, Life (Basel), 2020 May 14;10(5):E63, doi: 10,3390/life10050063,Terentyev et al, (2019), Carbonic anhydrase CAH3 supports the activity of photosystem II under increased pH, Biochim Biophys Acta Bioenerg, 2019 Jul 1;1860(7):582-590, doi: 10,1016/j,bbabio,2019,06,003,Correa-Galvis et al, (2016), Photosystem II Subunit PsbS Is Involved in the Induction of LHCSR Protein-dependent Energy Dissipation in Chlamydomonas reinhardtii, J Biol Chem, 2016 Aug 12;291(33):17478-87, doi: 10,1074/jbc,M116,737312,Mitchell et al, (2014), Dynamics of carbon concentrating mechanism induction and protein re-localisation during the dark to light transition in synchronised Chlamydomonas, Plant Physiol, 2014 Aug 8, pii: pp,114,246918,
Special application note:
This antibody recognizes native and recombinant carbonic anhydrase of Chlamydomonas reinhardtii,
Calcineurine is a calcium-binding protein involved in signal transduction,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Hordeum vulgare, Populus sp,, Triticum aestivum
Expected Species:
Brassica napus, Theobroma cacao Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from known calcineurin/EF-hand motif sequences including Arabidopsis thaliana CPK21, UniProt: Q9ZSA2, TAIR: AT4G04720 and fpound in other calcium-dependent protein kinases
Ascorbate peroxidase (APX) is the enzyme catalyzing the ascorbate-dependent reduction of hydrogen peroxide, Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in fruits and photosynthetic tissues, AA is found in every compartment of the plant cell including the apoplast,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7,4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Bègue et al, (2019), CDC48 regulates ascorbate peroxidase in tobacco, J Exp Bot, 2019 Mar 1, pii: erz097, doi: 10,1093/jxb/erz097,Wang et al, (2018), Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity, Proc Natl Acad Sci U S A, 2018 Nov 16, pii: 201813058, doi: 10,1073/pnas,1813058115, Balážová et al, (2018), Zinc oxide nanoparticles phytotoxicity on halophyte from genus Salicornia, Plant Physiol Biochem, 2018 Sep;130:30-42, doi: 10,1016/j,plaphy,2018,06,013,Adhikari et al, (2018), Sulfate improves cadmium tolerance by limiting cadmium accumulation, modulation of sulfur metabolism and antioxidant defense system in maize, Environmental and Experimental Botany Volume 153, September 2018, Pages 143-162,Ferrer et al, (2018), Differential Pb tolerance in metallicolous and non-metallicolous Zygophyllum fabago populations involves the strengthening of the antioxidative pathways, Environ & Exp Botany, Vo, 150, June 2018, Pages 141-151,Aroca et al, (2015), S-sulfhydration: a new post-translational modification in plant systems, Plant Physiology March 2015 pp,00009,2015,Terrile et al, (2014), Nitric oxide-mediated cell death is triggered by chitosan in F, eumartii spores, Pest Manag Sci, 2014 Apr 25, doi: 10,1002/ps,3814,Tsaniklidis et al, (2013), L-Ascorbic acid metabolism in parthenocarpic and seeded cherry tomatoes, Plant Growth Regul,DOI 10,1007/s10725-013-9845-0, (Solanum lycopersicum, immunolocalization)
Cat2 (Catalase 2) is an enzyme which occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Catalase is an enzyme found in most living organisms which is catalazying decomposition of hydrogen peroxide to water and oxygen, In plant cells catalase is found in peroxisomes, This enzyme is involved in photorespiration and symbiotic nitrogen fixation,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
To obtain reactivity with Solanum lycopersicum urea gel needs to be apply, Please, contact us for more details,To decrease background signal this antibody needs to be incubated in PBS-T NOT TBS-T, For reference, check image in application example below,
Bapatla et al, (2021), Modulation of Photorespiratory Enzymes by Oxidative and Photo-Oxidative Stress Induced by Menadione in Leaves of Pea (Pisum sativum), Plants 10, no, 5: 987, https://doi,org/10,3390/plants10050987Wilmowicz et al (2021) EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers, International Journal of Molecular Sciences, 2021; 22(6):3001, https://doi,org/10,3390/ijms22063001Li et al, (2021) Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit, Hortic Res, 2021 Feb 1;8(1):31, doi: 10,1038/s41438-021-00470-w, PMID: 33518707; PMCID: PMC7848011,Tokarz et al, (2021), Stem Photosynthesis-A Key Element of Grass Pea (Lathyrus sativus L,) Acclimatisation to Salinity, Int J Mol Sci, 2021 Jan 12;22(2):685, doi: 10,3390/ijms22020685, PMID: 33445673; PMCID: PMC7828162,Santos et al,(2020), Diversity of banana diploid hybrids: An assessment based on a hydroponic system, Plant Breeding Volume 139, Issue 6, doi: 10,1111/pbr,12869
Special application note:
This antibody is recognizing all three isoforms of Arabidopsis thaliana catalase, Catalase-2 is a main isoform expressed in leaf tissue and localized to peroxisomes, This antibody contains 0,1 % ProClin,
CBP20 (Nuclear cap-binding protein subunit 2)is a component of the cap-binding complex (CBC), involved in various processes such as pre-mRNA splicing and RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs), Alternative names: 20 kDa nuclear cap-binding protein, NCBP 20 kDa subunit
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Glycne max, Hordeum vulgare, Lotus corniculatus, Nicotiana tabacum, Oryza sativa, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Zea mays Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide, derived with Arabidopsis thaliana CBP20 protein Q9xFD1, At5g44200
CBP80 is a component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, Alternative names: 80 kDa nuclear cap-binding protein, NCBP 80 kDa subunit, abscisic acid-hypersensitive protein 1, ABA-hypersensitive protein 1, Protein ENSALADA
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
CCA1 (Circadian clocl associated 1) encodes a transcriptional repressor that performs overlapping functions with LHY in a regulatory feedback loop that is closely associated with the circadian oscillator of Arabidopsis, Binds to the evening element in the promoter of TOC1 and represses TOC1 transcription, CCA1 and LHY colocalize in the nucleus and form heterodimers in vivo, CCA1 and LHY function synergistically in regulating circadian rhythms of Arabidopsis,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CCA1 protein sequence, UniProt:P92973, TAIR:AT2G46830
Important note about protein extractionTranscription factors are best isolated by freezing nucelar extract in liquid nitrogen after addition of extraction buffer, followed by thawing directly at 100°C heating block, not at RT, Centrifugation for 5 minutes will remove cell debris, Protein concenration is measure ad rest is frozen in liquid Nitrogen, in aliquots, Frozen samples are thawed at RT and loaded as soon as they are in liquid form, without heating, Such precautions are necessary to take to be able to detect a transcription factor using antibodies,
Application Details:
1: 500 (Co-IP), 1 : 500 (WB)
Purity:
Affinity purified serum in PBS, pH 7,4
Reconstitution:
For reconstitution add 50 µl of sterile water,
Related products:
AS13 2661 | Anti-LHY | Late elongated hypocotyl, rabbit antibodiesAS13 2646 | Anti-TOC1 | TIMING OF CAB EXPRESSION 1, rabbit antibodiesPlant protein extraction buffer Secondary antibodies
Molecular Weight:
67 | 80 kDa
Not reactive in:
Oryza sativa
Selected references:
Hung er al, (2018), The Arabidopsis LDL1/2-HDA6 histone modification complex is functionally associated with CCA1/LHY in regulation of circadian clock genes, Nucleic Acids Res, 2018 Aug 14, doi: 10,1093/nar/gky749,
Copper is an essential element for function of chloroplasts, It is a co-factor for superoxide dismutase (SOD) and for plastocyanin, Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets, In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site, The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD, The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Glycne max, Hordeum vulgare, Solanum tuberosum Species of your interest not listed? Contact us, tech@nktscientific.com
Immunogen:
The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5, fused to His-tag, Overexpressed using pET28a vector in Bl21 E, coli (Cd+) and purified using His-column, After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC,
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Speth et al, (2013), RACK1 scaffold proteins influence miRNA abundance in Arabidopsis, Plant J, Aug 13,Abdel-Ghany et al (2005), ,AtCCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7, FEBS Lett, 11:2307-12,
Programmed cell death (PCD) is a fundamentally important biological process required to maintain the integrity and homeostasis of multicellular organisms during normal development and as a response to adverse environments, Cdc2 kinase, plays a major role in driving the cell cycle, Synonymes (for Arabidopsis thaliana): CDC2A, CDKA-1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
collection of antibodies for DNA/RNA metabolism and cell cyclePlant protein extraction buffer Secondary antibodies
Molecular Weight:
34-36 (Zea mays)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Syu et al, (2014), Impacts of size and shape of silver nanoparticles on Arabidopsis plant growth and gene expression, Plant Physiology and Biochemistry 2014, In print,
CDPK (calcium-dependent protein kinase) may play a role in signal transfuction pathways,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Hordeum vulgare, Populus sp,, Triticum aestivum
Expected Species:
Fragaria ananassa, Malus domestica, Nicotiana attenuata, Oryza sativa, Vicia faba Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide conserved in isoform 1,2,3,4,7,8,10,11,14,20,25,30,32 of Arabidopsis thaliana CDPK kinases
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Siddiqui et al, (2020), Melatonin and calcium function synergistically to promote the resilience through ROS metabolism under arsenic-induced stress, Journal of Hazardous MaterialsVolume 398, 5 November 2020, 122882Ciesla et al, (2016) A Role for Barley Calcium-Dependent Protein Kinase CPK2a in the Response to Drought, Front Plant Sci, 2016 Oct 25;7:1550,
CERK1 (Chitin elicitor receptor kinase 1) is a Lysin motif (LysM) receptor kinase that functions as a cell surface receptor in chitin elicitor (chitooligosaccharides) signaling leading to innate immunity toward both biotic and abiotic stresses (e,g, tolerance to salinity, heavy-metal stresses, and Botrytis cinerea infection), Recognizes microbe-derived N-acetylglucosamine (NAG)-containing ligands, Involved in the resistance to pathogenic fungi,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
collection of antibodies to pathogen attackcollection of antibodies to hypoxiaPlant protein extraction buffer Secondary antibodies
Molecular Weight:
67 kDa
Not reactive in:
Nicotiana benthamiana, Solanum lycopersicum
Selected references:
Ngou et al, (2021) Mutual potentiation of plant immunity by cell-surface and intracellular receptors, Nature, 2021 Mar 10, doi: 10,1038/s41586-021-03315-7, Epub ahead of print, PMID: 33692545,
Special application note:
This product can be sold containing proclin if requested
CesA4 (IRX5) (EC=2,4,1,12) is a catalytic subunit of xylem-specific cellulose synthase enzyme, involved in secondary cell wall biosynthesis - cellulose synthase trerminal complexes, It interacts with CESA7 and CESA8 which is required for a functyional complex and localization in secondary cell wall deposition sites, Expressed in young plants, stems and flowers but not in leaves, roots and shoots, Amounts are increasing as stems mature, Synonymes: Protein IRREGULAR XYLEM 5, IRX5, NWS2,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Betula luminifera, Brassica napus, Capsella rubella, Eutrema salsugineum, Gossypium hirsutum, Nelumbo nucifera, Noccaea caerulescens, Vitis vinifera Species of your interest not listed? Contact us
Otulak-Kozieł et al, (2018), Plant Cell Wall Dynamics in Compatible and Incompatible Potato Response to Infection Caused by Potato Virus Y (PVYNTN), Int J Mol Sci, 2018 Mar 15;19(3), pii: E862, doi: 10,3390/ijms19030862,Tsuchiya et al, (2015), Distribution of XTH, expansin, and secondary-wall-related CesA in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum), Front Plant Sci, 2015 May 15;6:323, doi: 10,3389/fpls,2015,00323,
Special application note:
This antibody is detecting both, recombinant and edogenous CesA4 (IRX5) protein,
CesA7 (IRX3) is a catalytic subunit of xylem-specific cellulose synthase, involved in secondary cell wall biosynthesis - cellulose synthase trerminal complexes, It interacts with CESA4 and CESA8 which is required for a functyional complex and localization in secondary cell wall deposition sites, Occurs in secondary cell wall developing tissues such as xylems and interfascicular regions in young plants stems and flowers, Not expressed in leaves, roots and shoots, Synonymes: Protein IRREGULAR XYLEM 3, IRX3, Protein FRAGILE FIBER 5,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Solanum lycopersicum
Expected Species:
Brassica napus, Eucalyptus grandis, Nicotiana tabacum, Populus sp, Species of your interest not listed? Contact us
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Tsuchiya et al, (2015), Distribution of XTH, expansin, and secondary-wall-related CesA in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum), Front Plant Sci, 2015 May 15;6:323, doi: 10,3389/fpls,2015,00323,
CesA8 (IRX1) is a catalytic subunit of an enzyme involved in secondary cell wall biosynthesis - cellulose synthase trerminal complexes, It interacts with CESA4 and CESA7 which is required for a functyional complex, The protein is needed for xylem cell wall thickening, Occurs in secondary cell wall developing tissues such as xylems and interfascicular regions, Not expressed in leaves and not found in embryos, Synonymes: Protein IRREGULAR XYLEM 1, IRX1, Protein LEAF WILTING 2, LEW2 ,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Zhang et al, (2016), Golgi-localized STELLO proteins regulate the assembly and trafficking of cellulose synthase complexes in Arabidopsis, Nat Commun, 2016 Jun 9;7:11656, doi: 10,1038/ncomms11656,Tsuchiya et al, (2015), Distribution of XTH, expansin, and secondary-wall-related CesA in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum), Front Plant Sci, 2015 May 15;6:323, doi: 10,3389/fpls,2015,00323,
Special application note:
This antibody is detecting both, recombinant and edogenous CesA8 (IRX1) protein,
Fructose-1,6 bisphosphatase (FBPase) (EC=3,1,311) is one of the regulatory enzymes in the sucrose biosynthetic pathway, In non-photosynthetic tissues, it regulates the rate of gluconeogenesis, In photosynthetic tissues, two FBPase isozymes (chloroplastic and cytosolic) play key roles in carbon assimilation and metabolism, In photosynthetic tissues cFBPase (cytosolic fructose 1,6 bisphosphatase) converts triose phosphates from the chloroplast to sucrose during light hours, Alternative name: D-fructose-1,6-bisphosphate 1-phosphohydrolase
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Capsella rubella, Pisum sativum, Ricinus communis, Glycine max, Phaseolus vulgaris, Sesamum indicum, Spinacia oleracea, Populus trichocarpa, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
Overexpressed cytosolic fructose 1,6 bisphosphatase (cFBPase) derived from the sequence from Arabidopsis thaliana cFBPase UniProt: Q9MA79, TAIR: AT1G43670
This antibody does not react with chloroplastic form of FBPase,Will this antibody be good as a cytosolic (non-microsomal control) in Arabidopsis thaliana roots? Although it has never been tested there is every likelihood that cFBPase will be expressed at reasonable levels even in roots, Even though the biosynthetic flux through to Sucrose may not be high as in mesophyll cells, central metabolism will still be active in young roots and the Sucrose etc being supplied externally still needs to be utilised,
Application Details:
1 : 5 000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water
Related products:
Collection of other antibodies which can be used as markers of cytoplasmPlant protein extraction bufferSecondary antibodies
Molecular Weight:
45 | 37 kDa (Arabidopsis thaliana)
Not reactive in:
Chlamydomonas reinhardtii
Selected references:
Qian et al, (2021) OsFes1C, a potential nucleotide exchange factor for OsBiP1, is involved in the ER and salt stress responses, Plant Physiology, 2021;, kiab263, https://doi,org/10,1093/plphys/kiab263Shahbaz and Pilon (2019), Conserved Cu-MicroRNAs in Arabidopsis thaliana Function in Copper Economy under Deficiency, Plants (Basel), 2019 May 29;8(6), pii: E141, doi: 10,3390/plants8060141,Patir-Nebioglu et al, (2019), Pyrophosphate modulates plant stress responses via SUMOylation, Elife, 2019 Feb 20;8, pii: e44213, doi: 10,7554/eLife,44213,Liu et al, (2019), IMPORTIN ?4 mediates nuclear import of GRF-INTERACTING FACTORs to control ovule development in Arabidopsis, Plant Physiol, 2019 Jan 18, pii: pp,01135,2018, doi: 10,1104/pp,18,01135,Seguel et al, (2018), PROHIBITIN 3 forms complexes with ISOCHORISMATE SYNTHASE 1 to regulate stress-induced salicylic acid biosynthesis in Arabidopsis, Plant Physiol, Jan 2018, DOI:10,1104/pp,17,00941
Special application note:
Kinetic and allosteric properties of the plant cytosolic FBPase are remarkably similar to the mammalian and yeast FBPase, but differ greatly from those of the chloroplastic FBPase, The antibody could detect FBPase from the human COS-7 cell line transfected with FBP1 expressing vector,This product can be sold containing ProClin if requested,
CGL160 (Conserved in green lineage 160) is a protein encoded in the nucleus and localized in chloroplast, It interacts with components of the CF1 sub-complex, Synonymes: ATCGL160,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
part of Arabidopsis thaliana recombinant CGL160 derived from a following sequence UniProt: O82279, TAIR: At2g31040
AS08 370 | Anti-ATPase | ATP synthase, whole enzyme, rabbit antibodycollection of antibodies to GreenCut proteinsPlant and algal protein extraction buffer Secondary antibodies
Molecular Weight:
38,6 | 34 kDa (without transit peptide)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known,
Selected references:
Galvis et al, (2020), H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in chloroplast ATP synthase mutants, Plant Physiol, pp,01561,2019, doi: 10,1104/pp,19,01561,Fristedt et al, (2015), The Thylakoid Membrane Protein CGL160 Supports CF1CF0 ATP Synthase Accumulation in Arabidopsis thaliana, PLoS One, 2015 Apr 2;10(4):e0121658, doi: 10,1371/journal,pone,0121658,
CGL40 is a very conserved protein involved in photosynthesis and its function is far not identified,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Capsella rubella, Brassica pekinensis Species of your interest not listed? Contact us
Immunogen:
full length, recombinant CGL40, UniProt: Q8LFL5 , TAIR: At1g49975
CGL78 is a protein conserved in the green lineage and predicted to be located in the chloroplast, Member of the Ycf54 superfamily,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Please, omitt SDS from transfer buffer and reduce transfer time to 45 min, Nitrocellulose membrane is recommended and SDS is omitted to allow this LMW protein to bind tighter to the membrane,
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 200 µl of sterile water,
Related products:
AS06 122 | CRD1 | cyanobacterial homolog of plant CHL27 cyclasePlant and algal protein extraction buffer Secondary antibodies
Molecular Weight:
24 kDa
Not reactive in:
Hordeum vulgare
Selected references:
Hsieh et al, (2013), The Proteome of Copper, Iron, Zinc, and Manganese Micronutrient Deficiency in Chlamydomonas reinhardtii, Mol Cell Proteomics, 2013 Jan;12(1):65-86, doi: 10,1074/mcp,M112,021840, Epub 2012 Oct 13,
Special application note:
Currently this antibody has not been confirmed to detect CGL78 protein in Arabidopsis thaliana, If you are interested to use this antibody in Arabidopsis, please, contact us,
Gene At5g11840 is encoding a chloroplast localized protein, called also YCF36,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
higher plants, for specific species Species of your interest not listed? please inquire
Immunogen:
Full length, recombiant protein of Arabidopsis thaliana, UniProt: B6IDH6-1, TAIR:At5g11840
CGLD27 (CONSERVED IN THE GREEN LINEAGE AND DIATOMS 27) is required for growth in low iron conditions, Localized in chloroplasts, Mostly expressed in seeds, leaves and flowers and at a lower extent in roots,
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes,
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana (recombinant CGLD27)
Expected Species:
Chlamydomonas reinhardtiSpecies of your interest not listed? Contact us, tech@nktscientific.com
Immunogen:
thioredoxin fusion of CGLD27 of Arabidopsis thaliana, UniProt Q9FN15-1 TAIR At5g67370
