CD38 is a widely expressed 45 kDa transmembrane glycoprotein with a short cytoplasmic tail (aa 121), a transmembrane domain (aa 2242) and an extracellular domain (aa 43300) [1]. It is a multifunctional ectoenzyme catalysing multiple reactions and also has a role in signal transduction and calcium signaling.
In the brain CD38 is expressed in neurons, astrocytes and microglial cells and expression was found to increase under neuroinflammatory conditions indicating that it may have a role in the regulation of neuroinflammation. In Alzheimers disease, CD38 immunoreactivity is seen in intracellular tangles and neuropil threads [2].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
100 µg in 100 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
A synthetic peptide from the C-terminal region of human CD38
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
[1] Malavasi, F.; Deaglio, S.; Funaro, A.; Ferrero, E.; Horenstein, A.L.; Ortolan, E.; Vaisitti, T.; Aydin, S. Evolution and Function of the ADP Ribosyl Cyclase/CD38 Gene Family in Physiology and Pathology. Physiol. Rev. 2008, 88, 841886. [2] Otsuka K., Mizuguchi M., Aizawa T., Haga S., Sato M., Inoya H., Namba Y., Machinami R. Immunoreactivity in Alzheimers neurofibrillary tangles (abstract) Brain Pathol. 1994;4:558.
CD38 is a widely expressed 45 kDa transmembrane glycoprotein with a short cytoplasmic tail (aa 121), a transmembrane domain (aa 2242) and an extracellular domain (aa 43300) [1]. It is a multifunctional ectoenzyme catalysing multiple reactions and also has a role in signal transduction and calcium signaling.
In the brain CD38 is expressed in neurons, astrocytes and microglial cells and expression was found to increase under neuroinflammatory conditions indicating that it may have a role in the regulation of neuroinflammation. In Alzheimers disease, CD38 immunoreactivity is seen in intracellular tangles and neuropil threads [2].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
50 µg in 50 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
A synthetic peptide from the C-terminal region of human CD38
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
[1] Malavasi, F.; Deaglio, S.; Funaro, A.; Ferrero, E.; Horenstein, A.L.; Ortolan, E.; Vaisitti, T.; Aydin, S. Evolution and Function of the ADP Ribosyl Cyclase/CD38 Gene Family in Physiology and Pathology. Physiol. Rev. 2008, 88, 841886. [2] Otsuka K., Mizuguchi M., Aizawa T., Haga S., Sato M., Inoya H., Namba Y., Machinami R. Immunoreactivity in Alzheimers neurofibrillary tangles (abstract) Brain Pathol. 1994;4:558.
Lysosomal acid glucosylceramidase (GBA or Glucocerebrosidase) is the lysosomal hydrolase that hydrolyzes glucosylceramide (GC) and glucosylsphingosine (GS) to ceramide and sphingosine. It is a 536-amino-acid membrane-associated protein with a 39-amino-acid leader sequence that is cleaved to produce a 497-amino-acid mature protein.
Mutations in the GBA gene cause Gaucher disease, a lysosomal storage disease characterised by an accumulation of glucocerebrosides. Patients with Gaucher disease and heterozygous carriers are at increased risk of developing Parkinson's disease and Dementia with Lewy Bodies.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
100 µg in 100 µl PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Lysosomal acid glucosylceramidase (GBA or Glucocerebrosidase) is the lysosomal hydrolase that hydrolyzes glucosylceramide (GC) and glucosylsphingosine (GS) to ceramide and sphingosine. It is a 536-amino-acid membrane-associated protein with a 39-amino-acid leader sequence that is cleaved to produce a 497-amino-acid mature protein.
Mutations in the GBA gene cause Gaucher disease, a lysosomal storage disease characterised by an accumulation of glucocerebrosides. Patients with Gaucher disease and heterozygous carriers are at increased risk of developing Parkinson's disease and Dementia with Lewy Bodies.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
50 µg in 50 µl PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Histamine is an important messenger molecule and neuromodulator with four known G protein-coupled receptors (GPCRs), H1, H2, H3 and H4. Histamine receptor H3 (HRH3) is a presynaptic autoreceptor that regulates histamine release from histaminergic neurons via negative feedback [1] as well as a heteroreceptor that regulates the release of other neurotransmitters [2,3,4]. It is also expressed postsynaptically in the modulation of dopamine signalling.
HRH3 is expressed predominantly in the cortex, thalamus, hypothalamus, hippocampus, amygdala and basal ganglia. Dysregulated central histaminergic signalling has been associated with a number of psychiatric disorders including sleep disorders, ADHD, Alzheimer's disease, Parkinson's disease and schizophrenia.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
100 µg in 100 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Guinae Pig, Cat
Immunogen:
A synthetic peptide from the C-terminal region of human HRH3
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
[1] Morisset S, Rouleau A, Ligneau X, Gbahou F, Tardivel-Lacombe J, Stark H, Schunack W, Ganellin CR, Schwartz JC, Arrang JM. High constitutive activity of native H3 receptors regulates histamine neurons in brain. Nature. 2000; 408:860-4. [2] Schlicker E, Betz R, Gothert M. Histamine H3 receptor-mediated inhibition of serotonin release in the rat brain cortex. Naunyn Schmiedebergs Arch Pharmacol. 1988; 337:588-90. [3] Schlicker E, Fink K, Hinterthaner M, Gothert M. Inhibition of noradrenaline release in the rat brain cortex via presynaptic H3 receptors. Naunyn Schmiedebergs Arch Pharmacol. 1989; 340:633-8. [4] Schlicker E, Fink K, Detzner M, Gothert M. Histamine inhibits dopamine release in the mouse striatum via presynaptic H3 receptors. J Neural Transm Gen Sect. 1993; 93:1-10.
Histamine is an important messenger molecule and neuromodulator with four known G protein-coupled receptors (GPCRs), H1, H2, H3 and H4. Histamine receptor H3 (HRH3) is a presynaptic autoreceptor that regulates histamine release from histaminergic neurons via negative feedback [1] as well as a heteroreceptor that regulates the release of other neurotransmitters [2,3,4]. It is also expressed postsynaptically in the modulation of dopamine signalling.
HRH3 is expressed predominantly in the cortex, thalamus, hypothalamus, hippocampus, amygdala and basal ganglia. Dysregulated central histaminergic signalling has been associated with a number of psychiatric disorders including sleep disorders, ADHD, Alzheimer's disease, Parkinson's disease and schizophrenia.
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
50 µg in 50 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Guinae Pig, Cat
Immunogen:
A synthetic peptide from the C-terminal region of human HRH3
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Antibody Isotype:
IgG
Application Details:
WB 1:2000-5000. IHC 1:10-100.
Category:
Primary Antibodies
Other names:
HH3R, G-protein coupled receptor 97, GPCR97, H3R
Research Areas:
Neuroscience
NS Reagents Product Area:
Neuroscience
Molecular Weight:
49kDa
Subcellular location:
Plasma Membrane
Purification:
Affinity purification
References:
[1] Morisset S, Rouleau A, Ligneau X, Gbahou F, Tardivel-Lacombe J, Stark H, Schunack W, Ganellin CR, Schwartz JC, Arrang JM. High constitutive activity of native H3 receptors regulates histamine neurons in brain. Nature. 2000; 408:860-4. [2] Schlicker E, Betz R, Gothert M. Histamine H3 receptor-mediated inhibition of serotonin release in the rat brain cortex. Naunyn Schmiedebergs Arch Pharmacol. 1988; 337:588-90. [3] Schlicker E, Fink K, Hinterthaner M, Gothert M. Inhibition of noradrenaline release in the rat brain cortex via presynaptic H3 receptors. Naunyn Schmiedebergs Arch Pharmacol. 1989; 340:633-8. [4] Schlicker E, Fink K, Detzner M, Gothert M. Histamine inhibits dopamine release in the mouse striatum via presynaptic H3 receptors. J Neural Transm Gen Sect. 1993; 93:1-10.
Phospholipase C-gamma2 (PLCG2) PLCG2 is an enzyme mainly expressed in immune cells (including microglia) which are involved in innate immunity. It is involved in the transmembrane transduction of immune signals that determine the fate and function of various immune cell types.
PLCG2 has higher expression levels in pathologically afected brain regions in Alzheimers disease indicating that the immune system may play a key role in the development of Alzheimers disease and a polymorphism in phospholipase C-gamma 2 (PLCG2) has been reported to be protective against late onset Alzheimers disease (LOAD) [1].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
100 µg in 100 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Cat, Dog, Chimpanzee, Pig, Bovine
Immunogen:
Partial length recombinant human PLCG2 from the N-terminal region
If you would like further information regarding the immunogen used in the production of this antibody or have a query about if this antibody will bind to your protein/species please contact us and we can do the analysis for you.
[1] Sims R, van der Lee SJ, Naj AC, Bellenguez C, Badarinarayan N, Jakobsdottir J et al (2017) Rare coding variants in PLCG2, ABI3, and TREM2 implicate microglial-mediated innate immunity in Alzheimers disease. Nat Genet 49:13731384.
Phospholipase C-gamma2 (PLCG2) PLCG2 is an enzyme mainly expressed in immune cells (including microglia) which are involved in innate immunity. It is involved in the transmembrane transduction of immune signals that determine the fate and function of various immune cell types.
PLCG2 has higher expression levels in pathologically afected brain regions in Alzheimers disease indicating that the immune system may play a key role in the development of Alzheimers disease and a polymorphism in phospholipase C-gamma 2 (PLCG2) has been reported to be protective against late onset Alzheimers disease (LOAD) [1].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal Antibody
Format:
50 µg in 50 µl Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
Storage Temp:
Store at -20°C. Avoid freeze / thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Expected Species:
Cat, Dog, Chimpanzee, Pig, Bovine
Immunogen:
Partial length recombinant human PLCG2 from the N-terminal region
If you would like further information regarding the immunogen used in the production of this antibody or have a query about if this antibody will bind to your protein/species please contact us and we can do the analysis for you.
[1] Sims R, van der Lee SJ, Naj AC, Bellenguez C, Badarinarayan N, Jakobsdottir J et al (2017) Rare coding variants in PLCG2, ABI3, and TREM2 implicate microglial-mediated innate immunity in Alzheimers disease. Nat Genet 49:13731384.
Superoxide dismutase 1 (SOD1) is one of three superoxide dismutases with SOD1 being the principal cytoplasmic superoxide dismutase in humans. It binds copper and zinc ions for its activity and stability and plays a major role in redox potential regulation. It catalyses the transformation of the superoxide anion (O2?) into hydrogen peroxide. SOD1 variants are a common cause of familial amyotrophic lateral sclerosis (ALS).
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
100 µg in 100 µl PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Antibody Isotype:
IgG
Application Details:
WB 1:500-2000. IHC 1:50-200.
Category:
Primary Antibodies
Other names:
ALS, ALS1, HEL-S-44, IPOA, SOD, hSod1, homodimer
Research Areas:
Neuroscience
NS Reagents Product Area:
Neuroscience
Molecular Weight:
16kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Superoxide dismutase 1 (SOD1) is one of three superoxide dismutases with SOD1 being the principal cytoplasmic superoxide dismutase in humans. It binds copper and zinc ions for its activity and stability and plays a major role in redox potential regulation. It catalyses the transformation of the superoxide anion (O2?) into hydrogen peroxide. SOD1 variants are a common cause of familial amyotrophic lateral sclerosis (ALS).
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
50 µg in 50 µl PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
Antibody Isotype:
IgG
Application Details:
WB 1:500-2000. IHC 1:50-200.
Category:
Primary Antibodies
Other names:
ALS, ALS1, HEL-S-44, IPOA, SOD, hSod1, homodimer
Research Areas:
Neuroscience
NS Reagents Product Area:
Neuroscience
Molecular Weight:
16kDa (Intended as a general guide and does not allow for all isoforms and species variations)
TREM2 (Triggering receptor expressed on myeloid cells 2) is a cell surface transmembrane glycoprotein with an immunoglobulin like extracellular domain and a cytoplasmic tail [1]. It is expressed in myeloid cells including dendritic cells, granulocytes, and tissue-specific macrophages such as osteoclasts. In the brain, TREM2 is only expressed by microglia and in the central nervous system expression of TREM2 varies with higher expression in the hippocampus, spinal cord and white matter.
TREM2 plays a complex role in neuroinflammation with expression of TREM2 being upregulated in pathological conditions such as Parkinson's disease, Amyotrophic lateral sclerosis (ALS), stroke, traumatic brain injury and Alzheimer's Disease [2]. TREM2 has also been shown to have a role in potential mechanisms linking urban air pollution to Alzheimer's Disease through its involvement in the regulation of neuroinflammation [3].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
100 µg in 100 µl PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
Immunogen:
KLH conjugated synthetic peptide from the N-terminal region of human TREM2
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
TREM-2, TREM2a, TREM2b, TREM2c, Trggering receptor expressed on myeloid cells 2, Trggering receptor expressed on myeloid cells 2a, Triggering receptor expressed on monocytes 2
Research Areas:
Neuroscience
NS Reagents Product Area:
Neuroscience
Molecular Weight:
25kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Subcellular location:
Cell membrane, Secreted
Purification:
Affinity purification
References:
[1] Bouchon A, Dietrich J, Colonna M. Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes. J Immunol. 2000;164:49915.
[2] Gratuze, M., Leyns, C.E.G. & Holtzman, D.M. New insights into the role of TREM2 in Alzheimers disease. Mol Neurodegeneration 13, 66 (2018).
[3] Hendrik J. Greve, Christen L. Mumaw, Evan J. Messenger, Prasada R. S. Kodavanti, Joyce L. Royland, Urmila P. Kodavanti and Michelle L. Block. Diesel exhaust impairs TREM2 to dysregulate neuroinflammation. J Neuroinflammation. 2020; 17: 351.
TREM2 (Triggering receptor expressed on myeloid cells 2) is a cell surface transmembrane glycoprotein with an immunoglobulin like extracellular domain and a cytoplasmic tail [1]. It is expressed in myeloid cells including dendritic cells, granulocytes, and tissue-specific macrophages such as osteoclasts. In the brain, TREM2 is only expressed by microglia and in the central nervous system expression of TREM2 varies with higher expression in the hippocampus, spinal cord and white matter.
TREM2 plays a complex role in neuroinflammation with expression of TREM2 being upregulated in pathological conditions such as Parkinson's disease, Amyotrophic lateral sclerosis (ALS), stroke, traumatic brain injury and Alzheimer's Disease [2]. TREM2 has also been shown to have a role in potential mechanisms linking urban air pollution to Alzheimer's Disease through its involvement in the regulation of neuroinflammation [3].
Product Type:
NS Reagents Antibody
Antibody Type:
Polyclonal
Format:
50 µg in 50 µl PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
If you would like us to check if this antibody is likely to bind to this protein from a different species please contact us. We are happy to check for you.
Immunogen:
KLH conjugated synthetic peptide from the N-terminal region of human TREM2
If you would like further information regarding the immunogen used in the production of this antibody or have a query about whether this antibody will bind to your protein/species please contact us and we can do the analysis for you.
TREM-2, TREM2a, TREM2b, TREM2c, Trggering receptor expressed on myeloid cells 2, Trggering receptor expressed on myeloid cells 2a, Triggering receptor expressed on monocytes 2
Research Areas:
Neuroscience
NS Reagents Product Area:
Neuroscience
Molecular Weight:
25kDa (Intended as a general guide and does not allow for all isoforms and species variations)
Subcellular location:
Cell membrane, Secreted
Purification:
Affinity purification
References:
[1] Bouchon A, Dietrich J, Colonna M. Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes. J Immunol. 2000;164:49915.
[2] Gratuze, M., Leyns, C.E.G. & Holtzman, D.M. New insights into the role of TREM2 in Alzheimers disease. Mol Neurodegeneration 13, 66 (2018).
[3] Hendrik J. Greve, Christen L. Mumaw, Evan J. Messenger, Prasada R. S. Kodavanti, Joyce L. Royland, Urmila P. Kodavanti and Michelle L. Block. Diesel exhaust impairs TREM2 to dysregulate neuroinflammation. J Neuroinflammation. 2020; 17: 351.
The aqua mounting medium is a widely used for coverslipping from aqueous solutions and is non-fluorescing and has an antifade component to increase the viewing time of the specimen. Use Aqua-Poly/Mount with most fluorescent dyes and stains including DAB, Alkaline Phosphatase, Fast Red, AEC (aminoethylcarbazole) and a variety of other chromogens to enhance and retain fluorescent intensity. The mounting medium (water based) can be used for frozen sections, fat stains, chromogens for immunohistochemistry and in situ hybridization as well as other applications requiring a water-soluble mounting medium.
Principle of method:
Coverslipping from aqueous solutions
Reagents provided:
50 ml Mounting medium (water based), ready-to-use.
Storage and handling:
Store at room temperature
Procedure:
1. Prepare slides as required. 2. Prior to coverslipping rinse the slides in distilled or deionized water. 3. Place the bottle upside down with the end cap attached in a container before use. This will help clear of tiny bubbles. 4. Blot excess water from the slide without drying the tissue specimen. The tissue must be moist prior to mounting. 5. Apply enough drops of aqua mounting medium on the tissue section so that the tissue is completely covered. 6. Carefully lower the coverslip at an angle while gently applying pressure to force any excess medium and air bubbles away from the tissue and out from under the coverslip. 7. Slides can be dried at room temperature or at 4°C or in an oven but the temperature hight is depending what type of staining is used. 8. Allow the aqua mounting medium to dry before examine under the microscope.
The fragments in our 100bp ladder range from 100-1,500 base pairs, with the 500 and 1,500 base pair bands having increased intensity to act as key reference points. There are 11 bands in total.
The expected mass of DNA in each band (based on a total of 0.5 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE.
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 100bp ladder is provided ready to use with orange G & xylene cyanol FF included as tracking dyes.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
The fragments in our 100bp ladder range from 100-1,500 base pairs, with the 500 and 1,500 base pair bands having increased intensity to act as key reference points. There are 11 bands in total.
The expected mass of DNA in each band (based on a total of 0.5 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE. .
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 100bp ladder is provided ready to use with orange G & xylene cyanol FF included as tracking dyes.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
The fragments in our 1Kbp ladder range from 250-10,000 base pairs, with the 1K and 3K base pair bands having increased intensity to act as key reference points. There are 13 bands in total.
The expected mass of DNA in each band (based on a total of 0.5 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE.
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 1Kbp ladder is provided ready to use with bromophenol blue & xylene cyanol FF included as tracking dyes.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
The fragments in our 1Kbp ladder range from 250-10,000 base pairs, with the 1K and 3K base pair bands having increased intensity to act as key reference points. There are 13 bands in total.
The expected mass of DNA in each band (based on a total of 0.5 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE. .
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 1Kbp ladder is provided ready to use with bromophenol blue & xylene cyanol FF included as tracking dyes.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
The fragments in our 50bp ladder range from 50-1,500 base pairs, with the 200, 500 and 1,200 base pair bands having increased intensity to act as key reference points. There are 17 bands in total.
The expected mass of DNA in each band (based on a total of 0.56 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE. .
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 50bp ladder is provided ready to use with orange G as the tracking dye.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
The fragments in our 50bp ladder range from 50-1,500 base pairs, with the 200, 500 and 1,200 base pair bands having increased intensity to act as key reference points. There are 17 bands in total.
The expected mass of DNA in each band (based on a total of 0.56 ug in a 5ul load) is provided for approximating the mass of DNA in sample bands of a similar size.
Our DNA ladders are a combination of a plasmid digested DNA and PCR products, providing precise fragments.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE. .
Arrange for our DNA ladders to be in your stores and receive 5 vials of any of our DNA ladders for free. Email us for more details - tech@nktscientific.com.
Background Info:
Our 50bp ladder is provided ready to use with orange G as the tracking dye.
Product Type:
NS Reagents DNA Ladder
Format:
Buffer: 10mM Tris-HCl (pH 8.0) and 10mM EDTA
Storage Temp:
Store at 25°C, for 6 months or 4°C for a year. For long-term storage, store at -20°C.
The NSR Prestained Protein Ladder is a three colour protein standard with 13 prestained proteins covering molecular weights from 3.5 to 245 kilodaltons. It has been designed for use in most general applications for a protein ladder with a broad range of protein sizes, key reference bands and excellent Western transfer efficiency (tested with PVDF, nylon and nitrocellulose membranes).
Background Info:
Most of the proteins are covalently coupled with a blue chromophore apart from two reference bands, one stained red at 75 kDa and one stained green at 25 kDa for easy reading of the ladder and to aid in the monitoring of protein separation.
The NSR Prestained Protein Ladder is supplied ready to use in gel loading buffer.
PLEASE E-MAIL US TO REQUEST A FREE SAMPLE.
Arrange for our Protein ladder to be in your stores and receive a free vial. Email us for more details - tech@nktscientific.com.
Product Type:
NS Reagents Protein Ladder
Format:
Buffer: 20 mM Tris-phosphate, pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, 15 % (v/v) Glycerol.
Storage Temp:
Store at 25°C, for 2 weeks or 4°C for 3 months. For long-term storage, store at -20°C.
The shelf life is 1 year from receipt when stored frozen, however the NSR Prestained Protein Ladder should be stable for many years if stored frozen and excessive freeze thaw cycles are avoided.
Concentration: 0.1~0.4 mg/ml of each protein (set to give similar size bands for each protein).
Application Details:
2.5µl - 10µl per lane (recommended)
Category:
Protein Ladders
Other names:
Protein Marker, Protein Gel Marker
Research Areas:
Molecular Biology
NS Reagents Product Area:
Molecular Biology
Release Date:
Jan-20
Cookies:
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