Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.Source of HSP70 standard: recombinant Hsp70 of Arabidopsis thaliana UniProt: Q9LHA8, TAIR: AT3G12580
Product Type:
Antibody
Format:
Lyophilized in glycerol
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Concentration: after re-constitution with sterile milliQ water final concentration of the standard is 0.15 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Application Details:
Standard curve: 3 loads are recommended (eg 0.1, 0.2, 0.3 pmol). Adjust range to fit your samples and your experiment. For most applications a sample load of 0.2 μg of chlorophyll/well will give a HSP70 signal in this range.Positive control: a 2 μl load per well is optimal for most chemiluminescent detection systems. Higher standard concentration needs to be used to allow detection by Coomasie stains. Such gels with higher standard concentration can not be used for quantitation using chemiluminescence.This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently. Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
Reconstitution:
For reconstitution add 82 µl of steril water. Please notice that this product contains glycerol and might appear as liquid but is provided lyophilized.Final standard concentration is going to be 0.15 pmol/µl.
Related products:
Primary antibody matching HSP70 standard: AS08 371 | anti-HSP70 | heat shock protein 70 cytoplasmic, rabbit antibodycollection of other protein standardscollection of matching global antibodiesPlant and algal protein extraction buffer | AgriseraSuperDeal
Molecular Weight:
70 kDa
Special application note:
The HSP70 protein standard can be used in a combination with Agrisera global antibiodies (AS08 371) to quantitate HSP70 from a wide range of species. Global antibodies are raised against highly conserved amino acid sequence. Quantitative western blot: detailed method description, video tutorial
Thioredoxin-like protein HCF164, chloroplastic is involved in maintaining cell redox homeostasis. The portein displayes disulfide reductase activity that is involved in the biogenesis of the plastid cytochrome b6f complex. Protein is located in the thylakoid membrane with the C-terminal hydrophilic portion, containing the thioredoxin like domain, extending into the thylakoid lumen.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Lyophilized antibody can be stored at -20°C for up to 3 years. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Aegilops tauschii, Anthurium amnicola, Cajanus cajan, Cephalotus follicularis, Cicer arietinum, Corchorus olitorius, Cucumis melo, Dichanthelium oligosanthes, Glycine soja, Gossypium hirsutum, Medicago truncatula, Morus notabilis, Musa acuminata, Nelumbo nucifera, Nicotiana sylvestris, Nicotiana tabacum, Oryza sativa subsp. japonica, Saccharum hybrid cultivar R570, Solanum chacoense , Theobroma cacao, Vigna radiata var. radiata, Zea mays Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from Arabidopsis thaliana HCF164 protein sequence, UniProt: A0A178V430,, TAIR: AT4G37200
STN7 (serine/threonine protein kinase) (EC=2.7.11.1) in Arabidopsis thaliana, and its Stt7 homologue in Chlamydomonas reinhardtii, is required for LHCII phosphorylation and for state transitions. The loss of this thylakoid-associated kinase blocked stn7 (stt7)-deficient mutants in state 1. Alternative names: Protein STATE TRANSITION 7, Stt7 homolog.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Nicotiana tabacum
Expected Species:
Anthurium amnicola, Arabidopsis alpina, Capsicum annuum, Dichanthelium oligosanthes, Glycine soja, Gossypium hirsutum, Hordeum vulgare, Medicago truncatula, Morus notabilis, Nelumbo nucifera, Nicotiana sylvestris, Noccaea caerulescens, Vigna radiata var. Radiata Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide specific for Arabidopsis thaliana STN7 serine/threonine kinase, UniProt: Q9S713, TAIR: At1g68830
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Rudenko et al. (2019). The role of carbonic anhydrase �±-CA4 in the adaptive reactions of photosynthetic apparatus: the study with �±-CA4 knockout plants. Protoplasma (2019). https://doi.org/10.1007/s00709-019-01456-1Pralon et al. (2019). Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019 Jun 20;2:220. doi: 10.1038/s42003-019-0477-4. Ancin et al. (2019). Overexpression of thioredoxin m in tobacco chloroplasts inhibits the protein kinase STN7 and alters photosynthetic performance. J Exp Bot. 2019 Feb 5;70(3):1005-1016. doi: 10.1093/jxb/ery415.Nikkanen et al. (2018). Multilevel regulation of non-photochemical quenching and state transitions by chloroplast NADPH-dependent thioredoxin reductase. Physiol Plant. 2018 Dec 22. doi: 10.1111/ppl.12914.Ancin et al. (2018). Thioredoxin m overexpression in tobacco chloroplasts inhibits the protein kinase STN7 and alters photosynthetic performance. J Exp Bot. 2018 Nov 21. doi: 10.1093/jxb/ery415.
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
STO1 (Stomatin-like protein 1) involved in mitochondrial respiratory chain supercomplex assembly.Protein is expressed in: cotyledon, guard cell, hypocotyl, plant callus, pollen, rosette leaf, vascular leaf. Alternative names: ATSLP1, SLP1, SPFH/Band 7/PHB domain-containing membrane-associated protein family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Noccaea caerulescens Species of your interest not listed? Contact us
Serine hydroxymethyltransferase (SHMT) is part of the mitochondrial enzyme complex.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Chlamydomonas reinhardii, Citrus unshiu, Glycine max, Hordeum vulgare, Medicago truncatula, Populus balsamifera, Ricinus communis, Solanum tuberosum, Vitis vinifera Species of your interest not listed? Contact us
This antibody can be used on total leaf extracts and isolated mitochondria
Application Details:
1: 1000 to 1 : 5 000 (WB)
Purity:
Total IgG
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
AS05 074 | Anti-GDC-H rabbit antibodies, marker of mitochondrial matrixAS06 203A | Anti- Idh | isocytrate dehydrogenase rabbit antibodies, marker of mitochondrial matrixAS07 212 | Anti-VDAC1 marker for mitochondrial outer membrane, rabbit antibodiesPlant protein extraction buffer Secondary antibodies
Molecular Weight:
53 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Rurek et al. (2018). Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought. Impaired Coordination of Selected Transcript and Proteomic Responses, and Regulation of Various Multifunctional Proteins, Int. J. Mol. Sci. 2018, 19(4), 1130; doi:10.3390/ijms19041130 (Special issue: Plant Mitochondria)Rurek et al. (2018). Cold and Heat Stress Diversely Alter Both Cauliflower Respiration and Distinct Mitochondrial Proteins Including OXPHOS Components and Matrix Enzymes, Int. J. Mol. Sci. 2018, 19(3), 877; doi:10.3390/ijms19030877 (Special issue: Plant Mitochondria)Yin et al. (2016). Comprehensive Mitochondrial Metabolic Shift during the Critical Node of Seed Ageing in Rice. PLoS One. 2016 Apr 28;11(4):e0148013. doi: 10.1371/journal.pone.0148013. eCollection 2016.Long et al. (2015). Contributions of photosynthetic and non-photosynthetic cell types to leaf respiration in Vicia faba L. and their responses to growth temperature. Plant Cell Environ. 2015 Apr 1. doi: 10.1111/pce.12544.Wei et al. (2013). Folate polyglutamylation eliminates dependence of activity on enzyme concentration in mitochondrial serine hydroxymethyltransferases from Arabidopsis thaliana. Arch Biochem Biophys 1;536(1):87-96.Camejo et al. (2012).Salinity-inducedchanges inS-nitrosylation ofpeamitochondrialproteins. J. Proteomics Dec.11.
NPR1 (regulatory protein NPR1) is a key positive regulator of the SA-dependent signaling pathway that negatively regulates JA-dependent signaling pathway. Controls the onset of systemic acquired resistance (SAR). Subcellular localization: cytoplasm, nucleaus (following induction by salicylic acid treatment or after pathogen infection. Alternative names: BTB/POZ domain-containing protein NPR1, Non-inducible immunity protein 1, Nim1, Salicylic acid insensitive 1, Sai1, ATNPR1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide, chosen from NPR1 sequence of Arabidopsis thaliana, TAIR: AT1G64280, UniProt: P93002
For successful detection using NPR1 antibody please follow protocol suggested below. NPR1 protein readily oligomerizes, in addition to any naturally occurring oligomer, during extraction. Therefore 50 mM DTT has to be used as well as denaturation at 75°C for 15 minutes. Engogenous NPR1 level is very low, thereofre SA treatment is absolutely necessary for good detection.This antibody is recognizing NPR1-GFP in the 35S overexpression line.
The major light-harvesting antenna complex II (LHCII) in photsynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. While Lhcb1 and Lhcb2 are quite similar and regularily present in multiple gene-copies, the Lhcb3 protein differs in pigment-composition and molecular size and often is coded by only a single gene. Lhcb3 seems not to be present in the mobile LHCII trimers involved in state 1-state 2 transitions. A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467–9476.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Cucumis melo, Dicots, Gymnosperms, Mosses Species of your interest not listed? Contact us
Immunogen:
BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana Lhcb3 UniProt: Q9S7M0,TAIR:AT5G54270. This sequence is highly conserved even in Ginko biloba and one of the major LHCII-forms of Physcomitrella patens.
Protein is processed into mature form (Jansson 1999).
Application Details:
1 : 2000 (WB)
Purity:
Total IgG
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
AS01 002PRE | Lhcb3 | LHCII type III chlorophyll a/b-binding protein, pre-immune serumAS01 003 | Anti-Lhcb2 | LHCII type II chlorophyll a/b-binding protein, rabbit antibodiesAS01 004 | Anti-Lhcb1 | LHCII type I chlorophyll a/b-binding protein, rabbit antibodiesPlant protein extraction buffer | AgriseraSuperDeal
Molecular Weight:
28.7 | 26 kDa for Arabidopsis thaliana
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Furukawa et al. (2019). Formation of a PSI–PSII megacomplex containing LHCSR and PsbS in the moss Physcomitrella patens. J Plant Res https://doi.org/10.1007/s10265-019-01138-2.Lv et al. (2019). Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. Plant Cell. 2019 Jan;31(1):210-230. doi: 10.1105/tpc.18.00813.Rogowski et al. (2019). Photosynthesis and organization of maize mesophyll and bundle sheath thylakoids of plants grown in various light intensities. Environmental and Experimental Botany Volume 162, June 2019, Pages 72-86.Mao et al. (2018). Comparison on Photosynthesis and Antioxidant Defense Systems in Wheat with Different Ploidy Levels and Octoploid Triticale. Int J Mol Sci. 2018 Oct 2;19(10). pii: E3006. doi: 10.3390/ijms19103006.Rantala and Tikkanen et al. (2018). Phosphorylation‐induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2.Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782.Shin et al. (2017), Complementation of a mutation in CpSRP43 causing partial truncation of light-harvesting chlorophyll antenna in Chlorella vulgaris. Sci Rep. 2017 Dec 20;7(1):17929. doi:10.1038/s41598-017-18221-0.Tyuereva et al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot)Yang-Er Chen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.Rozpądek et al. (2015). The fungal endophyte Epichloë typhina improves photosynthesis efficiency of its host orchard grass (Dactylis glomerata). Planta. 2015 Jun 10.Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675.Yao et al. (2015). Ultraviolet-B protection of ascorbate and tocopherol in plants related with their function on the stability on carotenoid and phenylpropanoid compounds. Plant Physiology and Biochemistry Volume 90, May 2015, Pages 23–31.Kunugi et al. (2016). Evolution of Green Plants Accompanied Changes in Light-Harvesting Systems. Plant Cell Physiol. 2016 Apr 6. pii: pcw071. Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.Wientjes et al (2013). LHCII is an antenna of both photosystems after long-term acclimation. BBA, Jan 6.Rudowska et al. (2012). Chloroplast biogenesis - correlation between structure and function. BBA, available on line, March 2012.
Special application note:
Antibody format is a total IgG fraction, which means that it is a pool of polyclonal antibodies obtained by purification of serum on Protein G, not on a specific antigen column.
The light-harvesting protein Lhca1 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca1 is imported as a precursor from the cytosol into the chloroplast. Upon insertion into the thylakoid membrane Lhca1 forms a heterodimer (LHCI-730) with Lhca4 that associates with the PSI core close to PsaG and PsaF.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065–3073
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4.
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
BSA-conjugated synthetic peptide derived from the Lhca1 protein of Arabidopsis thaliana UniProt: Q01667, TAIR: At3g54890. This sequence is highly conserved in Lhca1 proteins of angiosperms (monocots and dicots) and gymnosperms.
Protein is processed into mature form (Jansson 1999).
Application Details:
1 : 2000-1 : 5000 (WB)
Purity:
Total IgG
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
AS01 011 | A set of 10 plant anti-Lhca and anti-Lhcb antibodiesAS01 011 Chlamydomonas | A set of anti-Lhc antibodies for ChlamydmonasAvailable antibodies against pigment-binding proteins- LHCrecommended secondary antibodyPlant protein extraction buffer | AgriseraSuperDeal
Molecular Weight:
25.99 | 22 kDa for Arabidopsis thaliana
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Zhu et al. (2020). A NAC transcription factor and its interaction protein hinder abscisic acid biosynthesis by synergistically repressing NCED5 in Citrus reticulata. J Exp Bot. 2020 Jun 22;71(12):3613-3625.doi: 10.1093/jxb/eraa118. Forlani et al. (2020. HEBE, a novel positive regulator of senescence in Solanum lycopersicum. Sci Rep. 2020 Jul 3;10(1):11021.doi: 10.1038/s41598-020-67937-z. Wang et al. (2020). Post-translational coordination of chlorophyll biosynthesis and breakdown by BCMs maintains chlorophyll homeostasis during leaf development. Nat Commun. 2020; 11: 1254. Chen et al. (2019). Effects of Stripe Rust Infection on the Levels of Redox Balance and Photosynthetic Capacities in Wheat. Int J Mol Sci. 2019 Dec 31;21(1). pii: E268. doi: 10.3390/ijms21010268.Krupinska et al. (2019). The nucleoid-associated protein WHIRLY1 is required for the coordinate assembly of plastid and nucleus-encoded proteins during chloroplast development. Planta. 2019 Jan 11. doi: 10.1007/s00425-018-03085-z.Mao et al. (2018). Comparison on Photosynthesis and Antioxidant Defense Systems in Wheat with Different Ploidy Levels and Octoploid Triticale. Int J Mol Sci. 2018 Oct 2;19(10). pii: E3006. doi: 10.3390/ijms19103006.Yoshida et al. (2018). Thioredoxin-like2/2-Cys peroxiredoxin redox cascade supports oxidative thiol modulation in chloroplasts. Proc Natl Acad Sci U S A. 2018 Aug 13. pii: 201808284. doi: 10.1073/pnas.1808284115.Li et al. (2018). Modulating plant growth-metabolism coordination for sustainable agriculture. Nature. 2018 Aug 15. doi: 10.1038/s41586-018-0415-5.Zhu et al. (2018). A comprehensive proteomic analysis of elaioplasts from citrus fruits reveals insights into elaioplast biogenesis and function. Hortic Res. 2018 Feb 7;5:6. doi: 10.1038/s41438-017-0014-x.Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782. Schöttler et al. (2017). The plastid-encoded PsaI subunit stabilizes photosystem I during leaf senescence in tobacco. J Exp Bot. 2017 Feb 1;68(5):1137-1155. doi: 10.1093/jxb/erx009.Yang et al. (2017). Tetratricopeptide repeat protein Pyg7 is essential for photosystem I assembly by interacting with PsaC in Arabidopsis. Plant J. 2017 Jun 21. doi: 10.1111/tpj.13618.Tyuereva et al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot)Mazur et al. (2016). Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. BMC Plant Biol. 2016 Sep 2;16(1):191. doi: 10.1186/s12870-016-0883-4.Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658. Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.Saito et al. (2014). Fe deficiency induces phosphorylation and translocation of Lhcb1 in barley thylakoid membranes. FEBS Lett. 2014 May 8. pii: S0014-5793(14)00317-2. doi: 10.1016/j.febslet.2014.04.031.
Special application note:
Antibody format is a total IgG fraction, which means that it is a pool of polyclonal antibodies obtained by purification of serum on Protein G, not on a specific antigen column.This product can be sold containing ProClin if requested.
The light-harvesting protein Lhca2 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca2 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca2 forms a heterodimer (LHCI-680) with Lhca3 that associates with the PSI core close to PsaF and PsaK.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065–3073.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4.
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Arachis hypogaea, Bryopsis corticulans, Colobanthus quitensis Kunt Bartl, Chlamydomonas reinhardti (one Lhca-type), Citrus reticulata, Chromochloris zofingiensis, Cytisus cantabricus (Wilk.) Rchb. F., Hieracium pilosella L, Hordeum vulgare, Lasallia hispanica, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Physcomitrella patens, Pinus banksiana (the higher of the two bands detected at 24 and 30 kDa is not considered to be specific to any Lhc protein), Posidonia oceanica, Prasinoderma sp., Pyramimonas sp. Spinacia oleracea, Syntrichia muralis (Hedw.) Raab, Triticum aestivum, Triticale, Zea mays
Expected Species:
Dicots, Gymnosperms
Immunogen:
BSA-conjugated synthetic peptide derived from the Lhca2 protein of Arabidopsis thaliana UniProt: Q9SYW8, Q8LCQ4, TAIR: At3g61470. This sequence is highly conserved in Lhca2 proteins of angiosperms (monocots and dicots) and gymnosperms as well as in At1g19150. This gene codes for the very low expressed Lhca6 protein which also has been denoted as Lhca2*1.
AS01 011 | A set of 10 plant anti-Lhca and anti-Lhcb antibodies (rabbit antibodies)AS01 011 Chlamydomonas | A set of anti-Lhc antibodies for Chlamydmonas (rabbit antibodies)Available antibodies against pigment-binding proteins- LHCPlant protein extraction buffer | AgriseraSuperDeal
Molecular Weight:
27.7 | 24 kDa for Arabidopsis thaliana
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Zhu et al. (2020). A NAC transcription factor and its interaction protein hinder abscisic acid biosynthesis by synergistically repressing NCED5 in Citrus reticulata. J Exp Bot. 2020 Jun 22;71(12):3613-3625.doi: 10.1093/jxb/eraa118. Their et al. (2020). VIPP2 interacts with VIPP1 and HSP22E/F at chloroplast membranes and modulates a retrograde signal for HSP22E/F gene expression. Plant Cell Environ. 2020 Jan 29. doi: 10.1111/pce.13732.Vojta and Fulgosi (2019). Topology of TROL protein in thylakoid membranes of Arabidopsis thaliana. Physiol Plant. 2019 Jan 20. doi: 10.1111/ppl.12927.Roth et al. (2019). Regulation of Oxygenic Photosynthesis during Trophic Transitions in the Green Alga Chromochloris zofingiensis. Plant Cell. 2019 Feb 20. pii: tpc.00742.2018. doi: 10.1105/tpc.18.00742.Mao et al. (2018). Comparison on Photosynthesis and Antioxidant Defense Systems in Wheat with Different Ploidy Levels and Octoploid Triticale. Int J Mol Sci. 2018 Oct 2;19(10). pii: E3006. doi: 10.3390/ijms19103006.Kim et al. (2018). The rice zebra3 (z3) mutation disrupts citrate distribution and produces transverse dark-green/green variegation in mature leaves. Rice (N Y). 2018 Jan 5;11(1):1. doi: 10.1186/s12284-017-0196-8.Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782.Schöttler et al. (2017). The plastid-encoded PsaI subunit stabilizes photosystem I during leaf senescence in tobacco. J Exp Bot. 2017 Feb 1;68(5):1137-1155. doi: 10.1093/jxb/erx009.Chen et al. (2017). Comparison of Photosynthetic Characteristics and Antioxidant Systems in Different Wheat Strains. J Plant Growth Regul.Yang et al. (2017). Tetratricopeptide repeat protein Pyg7 is essential for photosystem I assembly by interacting with PsaC in Arabidopsis. Plant J. 2017 Jun 21. doi: 10.1111/tpj.13618.Míguez et al. (2017). Diversity of winter photoinhibitory responses: A case study in co-occurring lichens, mosses, herbs and woody plants from subalpine environments. Physiol Plant. 2017 Feb 14. doi: 10.1111/ppl.12551.Hu et al. (2017). The SUFBC2 D Complex is Required for the Biogenesis of All Major Classes of Plastid Fe-S Proteins. Plant J. 2017 Jan 19. doi: 10.1111/tpj.13483.Kunugi et al. (2016). Evolution of Green Plants Accompanied Changes in Light-Harvesting Systems. Plant Cell Physiol. 2016 Jun;57(6):1231-43. doi: 10.1093/pcp/pcw071. Epub 2016 Apr 6.Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.
Special application note:
Antibody format is a total IgG fraction, which means that it is a pool of polyclonal antibodies obtained by purification of serum on Protein G, not on a specific antigen column.
The light-harvesting protein Lhca3 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca3 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca3 forms a heterodimer (LHCI-680) with Lhca2 that associates with the PSI core close to PsaF and PsaK.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065–3073.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
BSA-conjugated synthetic peptide derived from the Lhca3 protein sequence from Arabidopsis thaliana UniProt: Q9SY97, TAIR: At1g61520. This sequence is highly conserved in Lhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms.
Protein is processed into mature form (Jansson 1999).
Application Details:
1 : 2000-1 : 5000 (WB)
Purity:
Total IgG
Reconstitution:
For reconstitution add 100 µl of sterile water
Related products:
AS01 011 | A set of 10 plant anti-Lhca and anti-Lhcb antibodiesAS01 011 Chlamydomonas | A set of anti-Lhc antibodies for ChlamydmonasAvailable antibodies against pigment-binding proteins- LHCPlant protein extraction buffer | AgriseraSuperDeal
Molecular Weight:
29 | 25 kDa for Arabidopsis thaliana (due to a transit peptide being cleaved off)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Zhu et al. (2020). A NAC transcription factor and its interaction protein hinder abscisic acid biosynthesis by synergistically repressing NCED5 in Citrus reticulata. J Exp Bot. 2020 Jun 22;71(12):3613-3625.doi: 10.1093/jxb/eraa118. Chen et al. (2019). Effects of Stripe Rust Infection on the Levels of Redox Balance and Photosynthetic Capacities in Wheat. Int J Mol Sci. 2019 Dec 31;21(1). pii: E268. doi: 10.3390/ijms21010268.Mao et al. (2018). Comparison on Photosynthesis and Antioxidant Defense Systems in Wheat with Different Ploidy Levels and Octoploid Triticale. Int J Mol Sci. 2018 Oct 2;19(10). pii: E3006. doi: 10.3390/ijms19103006.Li et al. (2018). Modulating plant growth-metabolism coordination for sustainable agriculture. Nature. 2018 Aug 15. doi: 10.1038/s41586-018-0415-5.Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782.Schöttler et al. (2017). The plastid-encoded PsaI subunit stabilizes photosystem I during leaf senescence in tobacco. J Exp Bot. 2017 Feb 1;68(5):1137-1155. doi: 10.1093/jxb/erx009.Chen et al. (2017). Comparison of Photosynthetic Characteristics and Antioxidant Systems in Different Wheat Strains. J Plant Growth Regul.Tyuereva et al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot)Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675.Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.
The light-harvesting protein Lhca4 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca4 is imported as a precursor from the cytosol into the chloroplast. Upon insertion into the thylakoid membrane Lhca4 forms a heterodimer (LHCI-730) with Lhca1 that associates with the PSI core close to PsaG and PsaF.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065–3073.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
BSA-conjugated synthetic peptide derived from the Lhca4 protein ofArabidopsis thaliana UniProt: P27521, TAIR: At3g47470. This sequence is highly conserved in Lhca4 proteins of angiosperms (monocots and dicots) and gymnosperms.
Protein is processed into mature form (Jansson 1999).
Application Details:
1 : 2000-1 : 5000 (WB)
Purity:
Total IgG
Reconstitution:
For reconstitution add 100 µl of sterile water
Related products:
AS01 011 | A set of 10 plant anti-Lhca and anti-Lhcb antibodiesAS01 011 Chlamydomonas | A set of anti-Lhc antibodies for ChlamydmonasAvailable antibodies against pigment-binding proteins- LHCrecommended secondary antibodyPlant protein extraction bufferSecondary antibodies | AgriseraSuperDeal
Molecular Weight:
27.7 | 21 kDa for Arabidopsis thaliana
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Zhu et al. (2020). A NAC transcription factor and its interaction protein hinder abscisic acid biosynthesis by synergistically repressing NCED5 in Citrus reticulata. J Exp Bot. 2020 Jun 22;71(12):3613-3625.doi: 10.1093/jxb/eraa118. Forlani et al. (2020. HEBE, a novel positive regulator of senescence in Solanum lycopersicum. Sci Rep. 2020 Jul 3;10(1):11021.doi: 10.1038/s41598-020-67937-z. Chen et al. (2019). Effects of Stripe Rust Infection on the Levels of Redox Balance and Photosynthetic Capacities in Wheat. Int J Mol Sci. 2019 Dec 31;21(1). pii: E268. doi: 10.3390/ijms21010268.Mao et al. (2018). Comparison on Photosynthesis and Antioxidant Defense Systems in Wheat with Different Ploidy Levels and Octoploid Triticale. Int J Mol Sci. 2018 Oct 2;19(10). pii: E3006. doi: 10.3390/ijms19103006.Li et al. (2018). Modulating plant growth-metabolism coordination for sustainable agriculture. Nature. 2018 Aug 15. doi: 10.1038/s41586-018-0415-5.Zhu et al. (2018). A comprehensive proteomic analysis of elaioplasts from citrus fruits reveals insights into elaioplast biogenesis and function. Hortic Res. 2018 Feb 7;5:6. doi: 10.1038/s41438-017-0014-x.Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782.Schöttler et al. (2017). The plastid-encoded PsaI subunit stabilizes photosystem I during leaf senescence in tobacco. J Exp Bot. 2017 Feb 1;68(5):1137-1155. doi: 10.1093/jxb/erx009.Tyuereva et al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot)Yang-Er Chen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.Nath et al. (2016). A Nitrogen-Fixing Subunit Essential for Accumulating 4Fe-4S-Containing Photosystem I Core Proteins. Plant Physiol. 2016 Dec;172(4):2459-2470.Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658. Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675.Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.
Lhcb4 (CP29) is one of the 3 minor chlrorophyll a/b-binding proteins associated with Photosystem II in plants and algae. Lhcb4 has been suggested to act in the regulation of the chl a excited state concentration of PSII because of its ability of sensing lumenal pH resulting in reversible phosphorylation. In Arabidopsis thaliana 2 genes code for two isoforms Lhcb4.1 and Lhcb4.2. A third isoform (Lhcb4.3, At2g40100), probably only present in dicots, has found to be differently regulated and therefore has been denoted as Lhcb8.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Catalpa bungei, Cucumis sativus, Populus, gymnosperms and microalgae Ostrecococcus tauri; the target sequence is only weakly conserved in Physcomitrella patensSpecies of your interest not listed? Contact us
Immunogen:
BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhb4 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana (Lhcb4.1 At5g01530 and Lhcb4.2 At3g08940 and Lhcb4.3 At2G40100).
AS06 117 | Anti-Lhcb4 | CP29 (Lhcb4) homolog, (Chlamydomonas), rabbit antibodiesLHC antibodies against pigment-binding proteinsPSII antibodies against Photosystem II proteinsAS04 045PRE Lhcb4 | CP29 chlorophyll a/b binding protein of plant PSII, pre-immune serumPlant protein extraction bufferSecondary antibodies | AgriseraSuperDeal
Molecular Weight:
31.9 | 29 kDa for Arabidopsis thaliana
Not reactive in:
Chlamydomonas reinhardtii (please use AS06 117 for this organism)
Selected references:
Grieco et al. (2020). Adjustment of photosynthetic activity to drought and fluctuating light in wheat. Plant Cell Environ. 2020 Mar 16. doi: 10.1111/pce.13756. Grimmer et al. (2020). Mild Proteasomal Stress Improves Photosynthetic Performance in Arabidopsis Chloroplasts. Nat Commun , 11 (1), 1662 Chen et al. (2019). Effects of Stripe Rust Infection on the Levels of Redox Balance and Photosynthetic Capacities in Wheat. Int J Mol Sci. 2019 Dec 31;21(1). pii: E268. doi: 10.3390/ijms21010268.Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658. Lv et al. (2019). Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. Plant Cell. 2019 Jan;31(1):210-230. doi: 10.1105/tpc.18.00813.Rogowski et al. (2019). Photosynthesis and organization of maize mesophyll and bundle sheath thylakoids of plants grown in various light intensities. Environmental and Experimental Botany Volume 162, June 2019, Pages 72-86.Lee et al. (2018). Prolines in Transit Peptides Are Crucial for Efficient Preprotein Translocation into Chloroplasts. Plant Physiol. 2018 Jan;176(1):663-677. doi: 10.1104/pp.17.01553. Epub 2017 Nov 20.Mao et al. (2018). Comparison on Photosynthesis and Antioxidant Defense Systems in Wheat with Different Ploidy Levels and Octoploid Triticale. Int J Mol Sci. 2018 Oct 2;19(10). pii: E3006. doi: 10.3390/ijms19103006.Geem at al. (2018). Jasmonic acid-inducible TSA1 facilitates ER body formation. Plant J. 2018 Sep 28. doi: 10.1111/tpj.14112.Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782.Kim et al. (2018). The rice zebra3 (z3) mutation disrupts citrate distribution and produces transverse dark-green/green variegation in mature leaves. Rice (N Y). 2018 Jan 5;11(1):1. doi: 10.1186/s12284-017-0196-8.Schöttler et al. (2017). The plastid-encoded PsaI subunit stabilizes photosystem I during leaf senescence in tobacco. J Exp Bot.Ã? 2017 Feb 1;68(5):1137-1155. doi: 10.1093/jxb/erx009.Tyuereva et al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot)Yang-Er Chen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.Betterle et al. (2016). The STN8 kinase-PBCP phosphatase system is responsible for high-light-induced reversible phosphorylation of the PSII inner antenna subunit CP29 in rice. Plant J. 2016 Nov 4. doi: 10.1111/tpj.13412. [Epub ahead of print]Pavlovič et al. (2016). A carnivorous sundew plant prefers protein over chitin as a source of nitrogen from its traps. Plant Physiol Biochem. 2016 Mar 5;104:11-16. doi: 10.1016/j.plaphy.2016.03.008Kim et al. (2015). Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015 Apr 16;6:6843. doi: 10.1038/ncomms7843.Sun et al. (2014). Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes. Biochim Biophys Acta. 2014 Nov 22. pii: S0005-2728(14)00650-1. doi: 10.1016/j.bbabio.2014.11.005.Grimmer et al. (2014). The RNA-binding protein RNP29 is an unusual Toc159 transport substrate. Front. Plant Sci. | doi: 10.3389/fpls.2014.00258
Special application note:
An overview about the different Lhc-protein types in plants can be found in Klimmek et al. (2006) Abundantly and rarely expressed Lhc protein genes exhibit distinct regulation patterns in plants. Plant Physiol 140: 793-804.Lhcb4 protein is processed into mature form (Jansson 1999).
Goat anti-rabbit IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Rabbit IgG (H&L)
Expected Species:
Rabbit IgG (H&L)
Immunogen:
Purified Rabbit IgG (H&L)
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.
Application Details:
The optimal working dilution should be determined by the investigator.
Purity:
Affinity purified goat IgG, F(ab)'2 fragment
Reconstitution:
For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Related products:
other antibodies to rabbit IgG HRP conjugated
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Linster et al. (2015). Downregulation of N-terminal acetylation triggers ABA-mediated drought responses in Arabidopsis. Nat Commun. 2015 Jul 17;6:7640. doi: 10.1038/ncomms8640.
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidasePurity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.
In plants the outer light-harvesting antenna of the photosystems (PSI and PSII) is composed of pigment-binding proteins belonging to the Lhc family. Of the 10 main conserved subtypes Lhca1-4 are exclusively associated with PSI while Lhcb3-6 are only found with PSII. Lhcb1 and Lhcb2 are found in the mobile trimers of LHCII associating with both photosystems in variable ratios (state1-state2 transitions). All Lhc polypeptides bind an individual set of chlorophyll a, chlorophyll b, and xanthophyll molecules in a structural and dynamic mutual arrangement which ensures high efficiency of energy transfer processes involved in light-harvesting and photoprotection.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Hordeum vulgare, Picea abies, Picea glauca, Pinus strobus, Spinacia oleracea, Drosera capensis. These antibodies have been shown to be reactive in all dicots, monocots, and gymnosperms
Immunogen:
BSA or KLH-conjugated synthetic peptides derived from conserved regions of plant Lhca1-4 and Lhcb1-6 protein sequences.
For reconstitution add sterile water according to label on each tube.
Related products:
Other antibodies to pigment-binding proteinsPlant protein extraction buffer
Molecular Weight:
20 - 29 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Merry et al. (2017). A comparison of pine and spruce in recovery from winter stress; changes in recovery kinetics, and the abundance and phosphorylation status of photosynthetic proteins during winter. Tree Physiol. 2017 Sep 1;37(9):1239-1250. doi: 10.1093/treephys/tpx065.Li et al. (2017). NYEs/SGRs-mediated chlorophyll degradation is critical for detoxification during seed maturation in Arabidopsis. Plant J. 2017 Nov;92(4):650-661. doi: 10.1111/tpj.13710. Epub 2017 Oct 20.Yoshida et al. (2016). Hisabori T1.Two distinct redox cascades cooperatively regulate chloroplast functions and sustain plant viability. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3967-76. doi: 10.1073/pnas.1604101113. Epub 2016 Jun 22.Pavlovič et al. (2016). A carnivorous sundew plant prefers protein over chitin as a source of nitrogen from its traps. Plant Physiol Biochem. 2016 Mar 5;104:11-16. doi: 10.1016/j.plaphy.2016.03.008Xu et al. (2011). Light-harvesting chlorophyll a/b-binding proteins are required for stomatal response to abscisic acid in Arabidopsis. J. Ex. Bot. Dec 5 (ahead of print).Kang et al. (2010). Evaluation of light-harvesting complex proteins as senescence-related protein markers in detached rice leaves. Photosynthetica 47, 4:638-640.
Special application note:
This set contains the following antibodies:Product number:Product name: AS01 005AS01 006AS01 007AS01 008AS01 004AS01 003AS01 002AS04 045AS01 009AS01 010Anti-Lhca1Anti-Lhca2Anti-Lhca3Anti-Lhca4Anti-LhcbAnti-Lhcb2Anti-Lhcb3Anti-Lhcb4Anti-Lhcb5Anti-Lhcb6
Donkey anti-rat IgG (H&L), is a secondary HRP (horse radish peroxidase) conjugated antibody which binds to rat IgGs in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
The optimal working dilution should be determined by the investigator.
Purity:
Affinity purified donkey IgG
Reconstitution:
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Related products:
other HRP conjugated secondary antibodies against rat IgG (H&L)
Selected references:
Bui et al. (2020). Differential submergence tolerance between juvenile and adult Arabidopsis plants involves the ANAC017 transcription factor. doi.org/10.1101/2020.02.12.945923 BioRxiv.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins by immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.
Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more. Alternative name: phosphatidylcholine phosphatido-hydrolase.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in phosphate buffer saline
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Brassica oleracea
Expected Species:
Arabidopsis thaliana, Cucumis melo, Ricinus communis, Populus balsamifera Species of your interest not listed? Contact us
Immunogen:
phospholipase D isolated and purified from Brassica oleracea
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.
Application Details:
1 : 100-1 : 1000 (ELISA), (IF), (IHC), (WB)
Purity:
Affinity purified IgG
Reconstitution:
For reconstitution add 0.5 ml of sterile destilled water.
Related products:
AS09 556 | Anti-PLD phospholipase D, rabbit antibodiesAS09 557 | Anti-PLD phospholipase D (biotinylated), rabbit antibodiesPlant protein extraction bufferSecondary antibodies
Molecular Weight:
92 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in phosphate buffer saline
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Canavalia ensiformis
Expected Species:
Arabidopsis thaliana, Glycine max, Ricinus communis Species of your interest not listed? Contact us
Immunogen:
urease isolated and purified from Canavalia ensiformis (common jack-beans)
Applications:
ELISA (ELISA), Western blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Goat anti-rabbit IgG, DyLight® 594 Conjugate is a secondary antibody conjugated to DyLight® 594, which binds to all rabbit IgGs in immunological assays.DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.Purity of this antibody is 95% based on SDS-PAGE.
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Related products:
other anti-rabbit DyLight® conjugated secondary antibodies Plant and algal protein extraction buffer
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Chong et al. (2015). Active fungal GH115 α-glucuronidase produced in Arabidopsis thaliana affects only the UX1-reactive glucuronate decorations on native glucuronoxylans. BMC Biotechnol. 2015 Jun 18;15:56. doi: 10.1186/s12896-015-0154-8.
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.DyLight® 594 (Ex = 593 nm; Em = 618 nm)
Donkey anti-rat IgG (H&L), FITC (fluorescein-5-isothiocyanate) conjugated, is a secondary antibody which binds to rat IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm.Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibodies are adsorbed against bovine,chicken,goat,guinea pig,hamster,horse,human,mouse, rabbi,sheep IgG and affinity purified using solid phase rat IgG.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C.Shelf life of this product is one year from date of receipt.
other FITC conjugated secondary antibodies to rat IgG
Selected references:
Wijnker et al. (2019). The Cdk1/Cdk2 homolog CDKA;1 controls the recombination landscape in Arabidopsis. Proc Natl Acad Sci U S A. 2019 Jun 18;116(25):12534-12539. doi: 10.1073/pnas.1820753116.
Special application note:
This antibody reacts with the heavy chains on rat IgG and with the light chains on all rat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin rat serum proteins or IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, or sheep based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7, 1% (w/v) BSA, Protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Based on IEP, this antibody Reacts with: Rabbit IgG heavy chainslight chains on all Rabbit immunoglobulins
Immunogen:
Purified Rabbit IgG, whole molecule.
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Long et al. (2020). FOXO3 is targeted by miR-223-3p and promotes osteogenic differentiation of bone marrow mesenchymal stem cells by enhancing autophagy. Hum Cell. 2020 Sep 13.doi: 10.1007/s13577-020-00421-y. Khajuria et al. (2020). Photochemical Efficiency Is Negatively Correlated With the Δ 9- Tetrahydrocannabinol Content in Cannabis Sativa L. Plant Physiol Biochem. 2020 Apr 8;151:589-600. doi: 10.1016/j.plaphy.2020.04.003.Ignatov et al. (2020). An mRNA-mRNA Interaction Couples Expression of a Virulence Factor and Its Chaperone in Listeria Monocytogenes. Cell Rep, 30 (12), 4027-4040.e7 Hu et al. (2019). Photoprotection capacity of microalgae improved by regulating the antenna size of light-harvesting complexes. J Appl Phycol (2019). doi.org/10.1007/s10811-019-01969-5Schober et al. (2019). Organelle Studies and Proteome Analyses on Mitochondria and Plastids Fractions from the Diatom Thalassiosira pseudonana. Plant Cell Physiol. 2019 Jun 10. pii: pcz097. doi: 10.1093/pcp/pcz097.Réthoré et al. (2019). Arabidopsis seedlings display a remarkable resilience under severe mineral starvation using their metabolic plasticity to remain self-sufficient for weeks. Plant J. 2019 Mar 22. doi: 10.1111/tpj.14325. Contreras et al. (2019). UV-B shock induces photoprotective flavonoids but not antioxidant activity in Antarctic Colobanthus quitensis (Kunth) Bartl. Environmental and Experimental Botany, Volume 159, March 2019, Pages 179-190. Nikkanen et al. (2018). Multilevel regulation of non-photochemical quenching and state transitions by chloroplast NADPH-dependent thioredoxin reductase. Physiol Plant. 2018 Dec 22. doi: 10.1111/ppl.12914.Migocka et al. (2018). Cucumber metal tolerance protein 7 (CsMTP7) is involved in the accumulation of Fe in mitochondria under Fe excess. Plant J. 2018 Jun 22. doi: 10.1111/tpj.14006. Tong et al. (2018). Delivery of siRNA in vitro and in vivo using PEI-capped porous silicon nanoparticles to silence MRP1 and inhibit proliferation in glioblastoma. J Nanobiotechnology. 2018 Apr 13;16(1):38. doi: 10.1186/s12951-018-0365-y. Nikkanen et al. (2018). Regulation of chloroplast NADH dehydrogenase-like complex by NADPH-dependent thioredoxin system. CSH, BioRixiv. doi.org/10.1101/261560. Sinclair et al. (2017) Etiolated Seedling Development Requires Repression of Photomorphogenesis by a Small Cell-Wall-Derived Dark Signal. Curr Biol. 2017 Nov 20;27(22):3403-3418.e7. doi: 10.1016/j.cub.2017.09.063.Gzyl et al. (2017). Gamma-tubulin distribution and ultrastructural changes in root cells of soybean (Glycine max L.) seedlings under cadmium stress. Environmental and Experimental Botany, Vol 143, Nov 2017, Pages 82-90. Kamies et al. (2017). A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef. Proteomes. 2017 Nov 15;5(4). pii: E32. doi: 10.3390/proteomes5040032. Niederhuber et al. (2017). Super-resolution microscopy of the ß-carboxysome reveals a homogenous matrix. Mol Biol Cell. 2017 Aug 9. pii: mbc.E17-01-0069. doi: 10.1091/mbc.E17-01-0069. Tyuereva et al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot) Romanowska et al. (2017). Differences in photosynthetic responses of NADP-ME type C4 species to high light. Planta. 2017 Mar;245(3):641-657. doi: 10.1007/s00425-016-2632-1. Epub 2016 Dec 18.Good et al. (2016). Attenuating Listeria monocytogenes Virulence by Targeting the Regulatory Protein PrfA. Cell Chem Biol. 2016 Mar 17;23(3):404-14. doi: 10.1016/j.chembiol.2016.02.013.Datta et al. (2016). Glutathione Regulates 1-Aminocyclopropane-1-Carboxylate Synthase Transcription via WRKY33 and 1-Aminocyclopropane-1-Carboxylate Oxidase by Modulating Messenger RNA Stability to Induce Ethylene Synthesis during Stress. Plant Physiol. 2015 Dec;169(4):2963-81. doi: 10.1104/pp.15.01543. Epub 2015 Oct 13.Barahimipour et al. (2016). Efficient expression of nuclear transgenes in the green alga Chlamydomonas: synthesis of an HIV antigen and development of a new selectable marker. Plant Mol Biol. 2016 Mar;90(4-5):403-18. doi: 10.1007/s11103-015-0425-8. Epub 2016 Jan 8.CiteAB has listed this antibody as one of the 7000 most published antibodies in the world.
Special application note:
Antibody is provided in: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1% BSA (w/v), Protease IgG free, 0.1 % (v/v) Kathon CG.Affinity purified antibody is > 95 % pure, according to SDS-PAGE.This antibody is used on a very wide range of samples from various species including many model plants, algae and diatoms.
Goat anti-chicken IgY (H&L) is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to chicken IgY in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Chicken IgY heavy and light chains (H&L).
Immunogen:
Purified chicken IgY, whole molecule.
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Antibody binds to:heavy chains on chicken IgYlight chains on all chicken immunoglobulinsNo reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis.Chicken immunoglobulin is often called hen or chicken IgG, however it is derived from egg yolk, therefore IgY.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Levitan et al. (2019). Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17316-17322. doi: 10.1073/pnas.1906726116.Heard et al. (2015). Identification of Regulatory and Cargo Proteins of Endosomal and Secretory Pathways in Arabidopsis thaliana by Proteomic Dissection. Mol Cell Proteomics. 2015 Jul;14(7):1796-813. doi: 10.1074/mcp.M115.050286. Epub 2015 Apr 21.Huang et al. (2015). Effects of exogenous salicylic acid on the physiological characteristics of Dendrobium officinale under chilling stress. Plant Growth Regulation pp 1-10.
Special application note:
This HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative.
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to AP or ALP (Alkaline phosphatase) which binds to all rabbit immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid, clear, colorless.
Storage Temp:
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Goat
Species Reactivity:
Rabbit IgG heavy and light chains (H&L).
Immunogen:
Purified Rabbit IgG, whole molecule.
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Based upon IEP, this antibody binds to: heavy chains on rabbit IgGlight chains on all rabbit immunoglobulinsNo reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Szymańska et al. (2019). SNF1-Related Protein Kinases SnRK2.4 and SnRK2.10 Modulate ROS Homeostasis in Plant Response to Salt Stress. Int J Mol Sci. 2019 Jan 2;20(1). pii: E143. doi: 10.3390/ijms20010143.Rozpądek et al. (2018). Acclimation of the photosynthetic apparatus and alterations in sugar metabolism in response to inoculation with endophytic fungi. Plant Cell Environ. 2018 Dec 5. doi: 10.1111/pce.13485.Borovik and Grabelnych (2018). Mitochondrial alternative cyanide-resistant oxidase is involved in an increase of heat stress tolerance in spring wheat. J Plant Physiol. 2018 Dec;231:310-317. doi: 10.1016/j.jplph.2018.10.007.Aswani et al. (2018). Oxidative stress induced in chloroplasts or mitochondria promotes proline accumulation in leaves of pea (Pisum sativum): another example of chloroplast-mitochondria interactions. Protoplasma. 2018 Sep 11. doi: 10.1007/s00709-018-1306-1.Giovanardi et al. (2018). In pea stipules a functional photosynthetic electron flow occurs despite a reduced dynamicity of LHCII association with photosystems. Biochim Biophys Acta. 2018 May 24. pii: S0005-2728(18)30129-4. doi: 10.1016/j.bbabio.2018.05.013.Hanschen et al. (2018). Differences in the enzymatic hydrolysis of glucosinolates increase the defense metabolite diversity in 19 Arabidopsis thaliana accessions. Plant Physiol Biochem. 2018 Mar;124:126-135. doi: 10.1016/j.plaphy.2018.01.009.Krasuska et al. (2015). Switch from heterotrophy to autotrophy of apple cotyledons depends on NO signal. Planta. 2015 Jul 18.
Special application note:
Antibody has been affinity purified on solid phase rabbit IgG (H&L).AP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative
AGO4 (Argonaute 4) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tubes.
AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca. 40 kDa.Note that the AGO4 antibody reacts with the NEB prestained protein marker.
Application Details:
5 µg of antibody per 1 gram of a fresh tissue (IP), 1 : 100 (ICC), 1 : 4000 (WB)
Zhai et al. (2015). A One Precursor One siRNA Model for Pol IV-Dependent siRNA Biogenesis. Cell. 2015 Oct 8;163(2):445-55. doi: 10.1016/j.cell.2015.09.032.Han et al. (2014). SUVR2 is involved in transcriptional gene silencing by associating with SNF2-related chromatin-remodeling proteins in Arabidopsis. Cell Res. 2014 Nov 25. doi: 10.1038/cr.2014.156. (coimmunoprecipitation)Zhang et al. (2013). DTF1 is a core component of RNA-directed DNA methylation and may assist in the recruitment of Pol IV. PNAS, May 14.Havecker et al. (2010) The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci. Plant Cell 22(2): 321-34.
Special application note:
Binds endogenous siRNAs, preference for 24nt siRNAs with 5' A.
NPR1 (regulatory protein NPR1) is a key positive regulator of the SA-dependent signaling pathway that negatively regulates JA-dependent signaling pathway. Controls the onset of systemic acquired resistance (SAR). Subcellular localization: cytoplasm, nucleaus (following induction by salicylic acid treatment or after pathogen infection. Alternative names: BTB/POZ domain-containing protein NPR1, Non-inducible immunity protein 1, Nim1, Salicylic acid insensitive 1, Sai1, ATNPR1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated peptide, chosen from NPR1 sequence of Arabidopsis thaliana, TAIR: AT1G64280, UniProt: P93002
For successful detection using NPR1 antibody please follow protocol suggested below. NPR1 protein readily oligomerizes, in addition to any naturally occurring oligomer, during extraction. Therefore 50 mM DTT has to be used as well as denaturation at 75°C for 15 minutes. Engogenous NPR1 level is very low, thereofre SA treatment is absolutely necessary for good detection.This antibody is recognizing NPR1-GFP in the 35S overexpression line.
Rabbit anti-mouse IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all mouse immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Host Animal:
Rabbit
Species Reactivity:
Mouse IgG heavy and light chains (H&L)
Expected Species:
Mouse IgG Heavy and Light chains (H&L)
Immunogen:
Purified Mouse IgG, whole molecule
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Bui et al. (2020). Differential submergence tolerance between juvenile and adult Arabidopsis plants involves the ANAC017 transcription factor. doi.org/10.1101/2020.02.12.945923 BioRxiv.Koch et al. (2019). Heat stress directly impairs gut integrity and recruits distinct immune cell populations into the bovine intestine. Proc Natl Acad Sci U S A. 2019 May 21;116(21):10333-10338. doi: 10.1073/pnas.1820130116.
Special application note:
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative.
Donkey anti-Rabbit IgG (H&L) - Affinity Pure, DyLight®633 Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human,mouse, rat or sheep IgG is a secondary antibody conjugated to DyLight® 633, which binds to rabbit IgG (H&L) in immunological assays.DyLight® 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are affinity purified using solid phase rabbit IgG (H&L).DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
immunolocalization Donkey anti-rabbit conjugated to DY Light 633 in the absence of primary antibody show very little background label (right image) and binds to primary antibodies (anti-Arf1 and anti-Sec21p; two left images). The top images show only the antibody label in red and bottom images show overlay of antibody label in red with DAPI in blue and DIC/Nomarski image in grey. The material has been prepared using whole mount immunolabelling procedure of 5 day old Arabidopsis thaliana seedlings (Fischer, U. et al. (2006). Curr. Biol. 16, 2143–2149). Antibody dilutions: rabbit anti-sec21p (Agrisera, AS08 327) 1:1000, rabbit anti-Arf1 (Agrisera, AS08 325) 1:1000 and Agrisera Donkey anti-rabbit DY Light 633 1:100. DNA was stained with DAPI. Courtesy: Dr. Anna Gustavsson and Prof. Markus Grebe, Umeå Plant Science Centre
Application Details:
1 : 20-1 : 2000 for most applications
Purity:
Affinity purified donkey IgG
Reconstitution:
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Related products:
other dylight anti-rabbit secondary antibodies
Special application note:
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, no reactivity is observed to:non-immunoglobulin rabbit serum proteinsIgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat.Based on immunoelectrophoresis, this antibody reacts with: (γ) chains on rabbit IgG light chains on all rabbit immunoglobulins.
Galactomannans are polysaccharides composed of a mannose backbone with side groups of galactose. They are used in food industry as stabilizers which increase water viscosity.Galactomannan is a component of the cell wall of the mold Aspergillus, and is released during its growth. Therefore, detection of this compound in blood is clinically used to diagnose aspergillosis infections in humans.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Cell culture supernatant, liquid
Storage Temp:
Antibody can be stored up to 1 month at 4°C, and over 1 month at -80°C. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
Arabidopsis thaliana, Cyamopsis tetragonoloba
Expected Species:
Guar and locust bean glycan group of galactomannan-1
Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more. Alternative name: phosphatidylcholine phosphatido-hydrolase.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in phosphate buffer saline
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Brassica oleracea
Expected Species:
Arabidopsis thaliana, Cucumis melo, Ricinus communis, Populus balsamifera Species of your interest not listed? Contact us
Immunogen:
phospholipase D isolated and purified from Brassica oleracea
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.
Application Details:
1 : 1000-1 : 20 000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG
Reconstitution:
For reconstitution add 0.5 ml of sterile destilled water.
Related products:
AS09 556 | Anti-PLD phospholipase D, rabbit antibodiesAS09 558 | Anti-PLD phospholipase D (affinity purified), rabbit antibodiesPlant protein extraction buffer
Molecular Weight:
92 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
V-ATPase subunit A is a catalytic subunit of V1 complex of vacuolar ATPase. This enzyme (EC=3.6.3.14) is involved in acidification process of various compartements of eucaryotic cell. This protein is coded by VHA-A gene. Alternative names: Vacuolar proton pump subunit alpha, vacuolar H(+)-ATPase subunit A, V-ATPase 69 kDa subunit
Product Type:
Antibody
Antibody Type:
Monoclonal, clone 7A5
Format:
Liquid
Storage Temp:
Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
No confirmed exceptions from predicted reactivity are currently known
Selected references:
Ward et al. (1992). Dissociation and Reassembly of the Vacuolar H-ATPase Complex from Oat Roots. Plant Physiol . 1992 May;99(1):161-9. doi: 10.1104/pp.99.1.161.
CNX (calnexin homolog) is a calcium-binding protein involved in protein folding. It interacts with newly synthesized glycoproteins in the endoplasmic reticulum.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid cell culture supernatant.
Storage Temp:
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Oxalate oxidase (EC=1.2.3.4) belongs to the family of oxidoreductases, and acts on the aldehyde or oxo group of donor with oxygen as acceptor. Those reactions are specific forglyoxylate and dicarboxylate metabolism. Alternative names: oxygen oxidoreductase, aero-oxalo dehydrogenase, oxalic acid oxidase.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in phosphate buffer saline, labelled with biotin
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Hordeum vulgare
Expected Species:
Triticum aestivum, Oryza sativa Species of your interest not listed? Contact us
Immunogen:
native oxalate oxidase have been purified from barley seedlings
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Application Details:
1 : 1000-1 : 4000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG
Reconstitution:
For reconstitution please add 1 ml of sterile destilled water.
Related products:
Plant protein extraction bufferSecondary antibodies
Molecular Weight:
21 kDa
Not reactive in:
Arabidopsis thaliana
Special application note:
Biotin/IgG protein molar ratio (B/P) is approximately 6.6. No foreign proteins are added. Marker used for lebling is N-hydroxysuccinimidoBiotin.
Beta amylase (EC 3.2.1.2.) catalyzes the hydrolysis of the second alfa-1,4 glycosidic bond. Alternative names 1,4-alfa-D-glucan maltohydrolase, glycogenase,saccharogen. amylase).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS labelled with biotin
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Kappaphycus alvarezii, Solanum tuberosum
Expected Species:
Arabidopsis thaliana, Glycine max, Physcomitrella patens, Populus trichocarpa, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
Beta amylase isolated and purified from sweet potato UniProt:Q94EU9
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Application Details:
1 : 1000-1 : 4000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG
Reconstitution:
For reconstitution add 1 ml of sterile water.
Related products:
AS09 379 | Anti-beta amylase antibodiesPlant and algal protein extraction bufferSecondary antibodies
Molecular Weight:
60 kDa
Selected references:
Usuldin et al. (2017). Molecular investigation of carrageenan production in Kappaphycus alvarezii in different culture conditions: a proteomic approach. ournal of Applied Phycology, August 2017, Volume 29, Issue 4, pp 1989–2001. (Kappaphycus alvarezii)
Special application note:
Biotin/IgG protein molar ratio (B/P) is approximately 6.6. No foreign proteins are added. Marker used for lebling is N-hydroxysuccinimidoBiotin.
UniProt number:
Q94EU9
Agrisera
AS09 409
1 x 50 µl of AS03 037, 2 x 2 ml of AS08 300, 5 x 10 µl of AS09 602, 1 X 10 ml of AS16 ECL-N-10
The Rubisco quantitation kit is suitable for quantitation of Rubisco in plant and algal samples using quantitative immunoblotting. It contains anti-RbcL antibody, Rubisco protein standard and the Protein Extraction Buffer for quantitative protein extraction. This kit is a good alternative to quantification of Rubisco by ELISA as it allows separation of Rubisco from a total complex cell protein mixture, which can be troublesome in case of ELISA method.
Product Type:
Antibody
Antibody Type:
Polyclonal
Storage Temp:
RbcL antibody and protein standard: Please store at -20°C (6 month) or -80°C for long term storage(years). Please, avoid freezing and thawing of reconstituted antibodies, make aliquots instead.PEB extraction buffer:Stable at RT for at least 1 month. For short-term storage please stoore (1 month) at 4°C and for long term storage (1 year) at -20°C.
Alpha proteobacteria, Algae (brown and red), Beta-proteobacteria, Dicots, Conifers, Cryptomonad, Cyanobacteria, Gamma-proeobacteria, Liverworts, Mosses, Prochlorophytes, PelwitschiaSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide was used to elicit anti-RbcL antibody. No baculovirus was used in production of this product.
1 x 50 µl of AS03 037, RbcL | Rubisco large subunit, form I and form II (amount enough for 50-100 Western blots)1 x 100 µl of AS01 017S, Rubisco protein standard (0.15 pmoles / µl, amount enough for generation of standard curve in 34 assays (standard curve: 0.0625 pmoles, 0.125 pmoles, 0.25 pmoles)2 x 2 ml of AS08 300, Protein extraction buffer (amount enough for 48 isolations of plant material, using 500 µl 1x PEB for 100 mg fresh weight) or 120 isolations of algal material (using 200 µl 1x PEB for cell amounts corresponding to 4-10 µg total chlorophyll)5 x 10 µl of AS09 602, Goat anti-rabbit IgG (H&L), HRP conjugated(amount enough for 50-100 Western blots)1 X 10 ml of AS16 ECL-N-10, AgriseraECL Bright 10 ml (5 ml of component A + 5 ml of component B, trial pack)
Quantitative western blot: detailed method description, video tutorialDiscussion over some critical aspects of quantitative western blot: Heidebrecht et al. (2009). Improved semiquantitative Western blot technique with increased quantification range. J. Immunological Methods. 35:40-48.
Application Details:
1 : 5000-1 : 10000 (WB)
Related products:
Rubisco/Carbon metabolism antibody collectionAS15 2994 | Rubisco ELISA quantitation kit AS15 2955 | Anti-RbcL II | Rubisco large subunit, form II (50 µl), rabbit antibodiesAS15 2955S | RbcL II | Rubisco form II positive control/quantitation standardAS01 017 | Anti-RbcL | Rubisco large subunit, form I, chicken antibodiesAS01 017S | Rubisco protein standard for quantitative western blot or positive controlAS03 037PRE | Rubisco large subunit, pre-immune serumAS07 218 | Anti-Rubisco | 557 kDa hexadecamer, rabbit antibodies, to a whole protein AS07 259 | Anti-RbcS | Rubisco small subunit (SSU), rabbit antibodiesmatching secondary antibody
Defez et al. (2019). Bacterial IAA-Delivery into Medicago Root Nodules Triggers a Balanced Stimulation of C and N Metabolism Leading to a Biomass Increase. Microorganisms. 2019 Sep 29;7(10). pii: E403. doi: 10.3390/microorganisms7100403.Sorrentino et al. (2018). Performance of three cardoon cultivars in an industrial heavy metal-contaminated soil: Effects on morphology, cytology and photosynthesis. J Hazard Mater. 2018 Jun 5;351:131-137. doi: 10.1016/j.jhazmat.2018.02.044.Mota et al. (2015). Effects of heavy metals on Cyanothece sp. CCY 0110 growth, extracellular polymeric substances (EPS) production, ultrastructure and protein profiles. J Proteomics. 2015 Apr 29;120:75-94. doi: 10.1016/j.jprot.2015.03.004.Thamatrakoln et al. (2013). Death-specific protein in a marine diatom regulates photosynthetic responses to iron and light availability. Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20123-8. doi: 10.1073/pnas.1304727110. Supplemental material describes western blot quantification method.
SUMO1 - Small Ubiquitin-like Modifier ubiquitin like protein binds in reversible way to various protein targets and plays a role as a signaling regulator.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Glycine max, Nicotiana tabacum, Picea sitchensis, Pisum sativum, Populus trichocarpa, Solanum lycopersicum, Zea mays Species of your interest not listed? Contact us
Immunogen:
Recombinant proSUMO1 from Arabidopsis thaliana Q547B9, At4g26840 with a his tag
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Colignon et al. (2019). Dual coordination of the SUMOylation and phosphorylation pathways during the response to heat stress in Solanum tuberosum. Environmental and Experimental Botany Volume 162, June 2019, Pages 192-200.Rosa et al. (2018). Insights into the transcriptional and post-transcriptional regulation of the rice SUMOylation machinery and into the role of two rice SUMO proteases. BMC Plant Biol. 2018 Dec 12;18(1):349. doi: 10.1186/s12870-018-1547-3.Guo et al. (2017). Sumoylation stabilizes RACK1B and enhance its interaction with RAP2.6 in the abscisic acid response. Sci Rep. 2017 Mar 8;7:44090. doi: 10.1038/srep44090.Tomanov et al. (2014). Arabidopsis PIAL1 and 2 Promote SUMO Chain Formation as E4-Type SUMO Ligases and Are Involved in Stress Responses and Sulfur Metabolism. Plant Cell. 2014 Nov;26(11):4547-60. doi: 10.1105/tpc.114.131300.Liu et al. (2014). SUMO E3 ligase AtMMS21 is required for normal meiosis and gametophyte development in Arabidopsis. BMC Plant Biol. 2014 Jun 3;14:153. doi: 10.1186/1471-2229-14-153.Kong et al. (2014). Quantitative proteomics analysis reveals that the nuclear cap-binding complex proteins Arabidopsis CBP20 and CBP80 modulate the salt stress response. J Proteome Res. 2014 Apr 1.
Alpha-mannosidase cleaves the alpha form of mannose.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in phosphate buffer saline
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Canavalia ensiformis (common jack-bean)
Immunogen:
native alpha-mannosidase purified from jack beans
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
Application Details:
1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG
Reconstitution:
For reconstitution add 1 ml of sterile destilled water.
Related products:
AS09 453 | Anti-alpha-mannosidase (biotinylated), rabbit antibodiesAS09 650 | Anti-alpha-mannosidase (affinity purified), rabbit antibodiesPlant and algal protein extraction bufferSecondary antibodies
Not reactive in:
Arabidopsis thaliana
Special application note:
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Glyoxylate reductase (EC=1.1.1.26) is an enzyme from oxidoreductase family and participates in glyoxylate and dicarboxylate metabolism. Alternative names: 3-hydroxybutyrate dehydrogenase/ phosphogluconate dehydrogenase (decarboxylating).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in phosphate buffer saline
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Spinacia oleracea
Immunogen:
native glyoxylate reductase purified from spinach
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more. Alternative name: phosphatidylcholine phosphatido-hydrolase.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in phosphate buffer saline
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Brassica oleracea
Expected Species:
Arabidopsis thaliana, Cucumis melo, Ricinus communis, Populus balsamifera, Zea mays Species of your interest not listed? Contact us
Immunogen:
phospholipase D isolated and purified from Brassica oleracea
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
Application Details:
1 : 1000-1 : 7 000 (ELISA), (IF), (IHC), (WB)
Purity:
Purified IgG
Reconstitution:
For reconstitution add 1 ml of sterile destilled water.
Related products:
AS09 557 | Anti-PLD phospholipase D (biotinylated), rabbit anitbodiesAS09 558 | Anti-PLD phospholipase D (affinity purified), rabbit antibodiesAS12 2364 | Anti-PLDA1/2 | phospholipase D alpha 1/2, rabbit antibodies with a broader species reactivityPlant protein extraction bufferSecondary antibodies
Molecular Weight:
92 kDa
Not reactive in:
Corynebacterium pseudotuberculosis
Special application note:
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in phosphate buffer saline
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Canavalia ensiformis
Expected Species:
Arabidopsis thaliana, Glycine max, Ricinus communis Species of your interest not listed? Contact us
Immunogen:
urease isolated and purified from Canavalia ensiformis (common jack-beans)
Applications:
ELISA (ELISA), Western blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in phosphate buffer saline
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Canavalia ensiformis
Expected Species:
Arabidopsis thaliana, Glycine max, Ricinus communis Species of your interest not listed? Contact us
Immunogen:
urease isolated and purified from Canavalia ensiformis (common jack-beans)
Applications:
ELISA (ELISA), Western blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. Methylation of histone H3K4 is associated with activation of gene transcription.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Chlorophylls are one of the most abundant classes of natural pigments and have an essential role in radiant energy absorptions during photosynthesis in bacteria, algae, and higher plants. Chlorophylls occur as noncovalently bound components of pigment-protein complexes, chloroplast-localized light-harvesting antennas LHC and photosynthetic reaction centers of PSI and PSII. Upon illumination the arrest in chlorophyll biosynthesis is ended and the etiolated parts of the plant and enzymatic photoreduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide), catalysed by POR enzyme.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
AS05 067 | Anti-POR | protochlorophilide oxidoreductase rabbit antibodies, larger product sizeAS08 300 | protein extraction buffer for plant and algal samplesPlant protein extraction buffer Secondary antibodies
Molecular Weight:
36-37 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Liu et al. (2018). AhGLK1 affects chlorophyll biosynthesis and photosynthesis in peanut leaves during recovery from drought. Sci Rep. 2018 Feb 2;8(1):2250. doi: 10.1038/s41598-018-20542-7.Yang et al. (2018). Effect of interactions between light intensity and red-to- far-red ratio on the photosynthesis of soybean leaves under shade condition. photosynthesis of soybean leaves under shade condition. Environ. Exp. Bot. Volume 150, June 2018, Pages 79-87.Sakuraba et al. (2013). The rice faded green leaf locus encodes protochlorophyllideoxidoreductase B and is essential for chlorophyll synthesis under high light conditions. Plant J.Yuan et al. (2012).Assembly of NADPH:protochlorophyllideoxido reductase complex is needed for effective greening of barley seedlings. J. Plant Physiol. June 13.
ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Acinetobacter baumannii, Algae, Cannabis sativa, Clostridium sp., Diatoms, Cyanobacteria, E.coli K-12, Vitis vinifera, Yersinia sp. Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from available plant, algal (chloroplastic and mitochondrial) and bacterial sequences of beta subunits of F-type ATP synthases, including Arabidopsis thaliana chloroplastic ATP synthase subunit beta AtCg00480 and Arabidopsis thaliana mitochondrial ATP synthase subunit beta-1 At5g08670 as well as Chlamydomonas reinhardtii P06541 and A8IQU3
Applications:
Blue Native-PAGE (BN-PAGE), Immunofluorescence (IF), Western blot (WB)
Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria, Bacillus cereus.
Aihara et al. (2019). Algal photoprotection is regulated by the E3 ligase CUL4-DDB1DET1. Nat Plants. 2019 Jan;5(1):34-40. doi: 10.1038/s41477-018-0332-5.Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)Rurek et al. (2015). Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. Biochim Biophys Acta. 2015 Jan 21. pii: S0005-2728(15)00016-X. doi: 10.1016/j.bbabio.2015.01.005.Eom et al. (2014). Bacillus subtilis HJ18-4 from Traditional Fermented Soybean Food Inhibits Bacillus cereus Growth and Toxin-Related Genes. J Food Sci. 2014 Nov;79(11):M2279-87. doi: 10.1111/1750-3841.12569. Epub 2014 Oct 30.Lintala et al. (2013). Arabidopsis tic62 trol mutant lacking thylakoid bound ferredoxin-NADP+ oxidoreductase shows distinct metabolic phenotype. Mol Plant Sep 16.Teng et al. (2013). Mitochondrial Genes of Dinoflagellates Are Transcribed by a Nuclear-Encoded Single-Subunit RNA Polymerase. PLOS ONE, June 2013. (immuofluorescence)Rasala et al. (2013). Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii. Plant J. March 23.Heinnickel et al. (2013). Novel thylakoid membrane greencut protein cpld38 impacts accumulation of the cytochrome b6f complex and associated regulatory processes. J. Biol. Chem. Jan 9.
Special application note:
This product can be sold containing proClin if requested.The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 Atp Synthase, and most known bacterial F-type Atp Synthases. Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein.
Goat anti-rabbit IgG is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to all rabbit immunoglobulins in immunological assays.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store liquid material at 2-8°C upto 6 month.
Host Animal:
Goat
Species Reactivity:
Rabbit IgG heavy and light chains (H&L)
Expected Species:
Rabbit IgG Heavy and Light chains (H&L)
Immunogen:
Purified Rabbit IgG, whole molecule
Applications:
ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Hanschen et al. (2018). Differences in the enzymatic hydrolysis of glucosinolates increase the defense metabolite diversity in 19 Arabidopsis thaliana accessions. Plant Physiol Biochem. 2018 Mar;124:126-135. doi: 10.1016/j.plaphy.2018.01.009.Giovanardi et al. (2018). In pea stipules a functional photosynthetic electron flow occurs despite a reduced dynamicity of LHCII association with photosystems. Biochim Biophys Acta. 2018 May 24. pii: S0005-2728(18)30129-4. doi: 10.1016/j.bbabio.2018.05.013.Krasuska et al. (2015). Switch from heterotrophy to autotrophy of apple cotyledons depends on NO signal. Planta. 2015 Jul 18.
Special application note:
Antibody has been affinity purified on solid phase rabbit IgG (H&L)AP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative
40S ribosomal protein S14-1 is localized in cytoplasm and belongs to ribosomal protein S11P family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
20 µg of total protein is needed for detection of S14 in Arabidopsis thaliana
Application Details:
1 : 1000 (WB)
Purity:
Affinity purified serum in PBS pH 7.4
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
AS13 2650 | anti-L13-1 | 60S ribosomal protein L13-1, rabbit antibodies AS09 478| anti-L13-1 | 60S ribosomal protein L13-1, rabbit antibodies AS08 331| anti-L-30 | 50S ribosomal protein L30, rabbit antibodiesAS11 1738| anti-Rpl1 | 50S ribosomal protein L1, rabbit antibodies AS15 2876 | anti-RPL2 | ribosomal protein L2 (chloroplastic), rabbit antibodies AS14 2780 | anti-RPL33 | 50S ribosomal protein L33, chloroplastic, rabbit antibodies AS14 2781 | anti-RPL36 | 50S ribosomal protein L36, chloroplastic, rabbit antibodiesAS12 2115 | anti-RPL37 | ribosomal protein L37, cytoplasmic, rabbit antibodiesAS15 2875 | anti-RPS1 | ribosomal protein S1 (chloroplastic), rabbit antibodiesAS12 2114 | anti-RPS12 | ribosomal protein S12, chloroplastic, rabbit antibodiesAS14 2779 | anti-RPS15 | 30S ribosomal protein S15, chloroplastic, rabbit antibodiesAS08 309 | anti-S1 | anti-30S ribosomal protein S1, rabbit antibodiesAS15 3068 | anti-S4 | mitochondrial ribosomal small subunit protein S4, rabbit antibodiesAS15 3067 | anti-S10 | mitochondrial ribosomal small subunit protein S10, rabbit antibodiesAS15 3069 | anti-L16 mitochondrial ribosomal large subunit protein L16, rabbit antibodiesPlant and algal protein extraction bufferSecondary antibodies | AgriseraSuperDeal
Molecular Weight:
16 | 15 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Pereira Firmino et al. (2020). Separation and Paired Proteome Profiling of Plant Chloroplast and Cytoplasmic Ribosomes. Plants (Basel) . 2020 Jul 14;9(7):892.doi: 10.3390/plants9070892. Ma et al. (2020). An ortholog of the Vasa intronic gene is required for small RNA-mediated translation repression in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 2020 Jan 7;117(1):761-770. doi: 10.1073/pnas.1908356117.Shinozaki et al. (2020). Autophagy Increases Zinc Bioavailability to Avoid Light-Mediated ROS Production under Zn Deficiency. Plant Physiol. 2020 Jan 15. pii: pp.01522.2019. doi: 10.1104/pp.19.01522.Wegener et al. (2019). Magnetic Tracking of Protein Synthesis in Microfluidic Environments-Challenges and Perspectives. Nanomaterials (Basel). 2019 Apr 9;9(4). pii: E585. doi: 10.3390/nano9040585.Liu et al. (2018). Transcriptomics analyses reveal the molecular roadmap and long noncoding RNA landscape of sperm cell lineage development. Plant J. 2018 Jul 26. doi: 10.1111/tpj.14041.Linster et al. (2015). Downregulation of N-terminal acetylation triggers ABA-mediated drought responses in Arabidopsis. Nat Commun. 2015 Jul 17;6:7640. doi: 10.1038/ncomms8640.
1-aminocyclopropane-1-carboxylate synthase (ACS) enzymes catalyze the conversion of S-adenosyl-L-methionine (SAM) into 1-aminocyclopropane-1-carboxylate (ACC), a direct precursor of ethylene. (EC:4.4.1.14). Synonymes: S-adenosyl-L-methionine methylthioadenosine-lyase 7.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
AKIN10 (E.C.= 2.7.11.1) is a catalytic subunit of the putative trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. Synonymes: AKIN alpha-2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from C-terminal part of Arabidopsis thaliana AKIN10 sequence UniProt: Q38997, TAIR: At3g01090
AKIN10 antibody works best when first the SnRK1 complex is immunorecipitated with this or another antibody and then anti-AKIN10 antibody is used to probe with. When this antibody is used against a lysate, the dominant rubisco protein can mask any immunoreactivity.
Application Details:
1: 50 (IL), 1 : 500 (WB)
Purity:
Affinity purified serum in PBS, pH 7.4
Reconstitution:
For reconstitution add 50 µl/tube of sterile water.
Related products:
AS09 460 | Anti-AKINB1 | SNF1-related protein kinase regulatory subunit beta-1, rabbit antibodiesAS09 462 | Anti-AKINB2 | SNF1-related protein kinase regulatory subunit beta-2, rabbit antibodiesAS15 2859 | Anti-AKIN ß3 | SNF1-related protein kinase regulatory subunit beta-3, rabbit antibodiesAS09 463 | Anti-AKIN beta gamma, rabbit antibodiesAS10 920 | Anti-AKIN11 | SNF1-related protein kinase catalytic subunit alpha KIN11, rabbit antibodiesAS09 613 | Anti-AKING1 | SNF1-related protein kinase regulatory subunit gamma 1, rabbit antibodiescollection of antibodies to proteins involved in signal transductionPlant protein extraction buffer | AgriseraSuperDeal
Krasnoperova et al. (2019). Potential Involvement of KIN10 and KIN11 Catalytic Subunits of the SnRK1 Protein Kinase Complexes in the Regulation of Arabidopsis γ-Tubulin. Cytology and Genetics, Volume 53, Issue 5, pp 349–356. (immunolocalization)Blanco et al. (2019). Dual and dynamic intracellular localization of Arabidopsis thaliana SnRK1.1. J Exp Bot. 2019 Apr 15;70(8):2325-2338. doi: 10.1093/jxb/erz023.Frank et al. (2018). Circadian Entrainment in Arabidopsis by the Sugar-Responsive Transcription Factor bZIP63. Curr Biol. 2018 Aug 20;28(16):2597-2606.e6. doi: 10.1016/j.cub.2018.05.092.Chan et al. (2017). SnRK1 phosphorylation of FUSCA3 positively regulates embryogenesis, seed yield, and plant growth at high temperature in Arabidopsis. Journal of Experimental Botany, erx233, https://doi.org/10.1093/jxb/erx233.Nukarinen et al. (2016). Quantitative phosphoproteomics reveals the role of the AMPK plant ortholog SnRK1 as a metabolic master regulator under energy deprivation. Sci Rep. 2016 Aug 22;6:31697. doi: 10.1038/srep31697.Castro et al. (2015). SIZ1-Dependent Post-Translational Modification by SUMO Modulates Sugar Signalling and Metabolism in Arabidopsis thaliana. Plant Cell Physiol. 2015 Oct 14. pii: pcv149.Emanuelle et al. (2015). SnRK1 from Arabidopsis thaliana is an atypical AMPK. Plant J. 2015 Mar 3. doi: 10.1111/tpj.12813.Rodrigues et al. (2013). ABI1 and PP2CA Phosphatases Are Negative Regulators of Snf1-Related Protein Kinase1 Signaling in Arabidopsis. Plant Cell, Oct 31.
HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
depends on the MW of the protein which is HA-tagged
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Hwang et al. (2019) Arabidopsis ABF3 and ABF4 Transcription Factors Act with the NF-YC Complex to Regulate SOC1 Expression and Mediate Drought-Accelerated Flowering. Mol Plant. 2019 Apr 1;12(4):489-505. doi: 10.1016/j.molp.2019.01.002
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Clonorchis sinensis, Cucurbita maxima, Cucumis sativus, Genlisea aurea, Glycine soja, Gossypium arboreum, Gossypium hirsutum, Litsea cubeba, Medicago truncatula, Narcissus tazetta var. chinensis, Phaseolus vulgaris, Platycodon grandiflorus, Paulownia tomentosa, Populus trichocarpa, Phaseolus vulgaris, Prunus avium, Ricinus communis, Solanum tuberosum, Striga asiatica, Theobroma cacao Species of your interest not listed? Contact us
Immunogen:
Full length recombinant Arabidopsis thaliana Actin-1 (ACT1) expressed in E.coli, Uniprot: P0CJ46
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Kandasamy, M.K. et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell. 24, 2012 May;24(5):2041-57. doi: 10.1105/tpc.111.095281Kandasamy, M.K. et al. (2001). One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell. Jul;13(7):1541-54Kandasamy, M.K. et al. (1999). The late pollen-specific actins in angiosperms. Plant Journal. Jun;18(6):681-91.
Special application note:
This antibody is a total IgG fraction purified on a protein G column. It recognizes the epitope Asn79 of actins ACT1, 3, 4, 12.
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
Arabidopsis thaliana, Nicotiana tabacum
Expected Species:
Actinidia deliciosa, Brachypodium sylvaticum, Brassica napus, Brassica oleracea, Cucumis sativus, Dionea muscipula, Euphorbia lathyris, Ficus microcarpa, Gentlisea aurea, Gossypium sp., Hevea brasiliensis, Medicago sativa, Medicago truncatula, Oryza sativa subsp. japonica, Oxytropis ochrocephala, Panax notoginseng, Populus trichocarpa, Phaseolus vulgaris, Prunus avium, Striga asiatica, Theobrom cacao, Torenia fournieri, Trifolium pratense, Vaccinium ashei, Ziziphus jujuba Species of your interest not listed? Contact us
Immunogen:
Full length recombinant Arabidopsis thaliana Actin-2 (ACT2) expressed in E.coli, Uniprot: Q96292
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Kandasamy, M.K., et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell May;24(5):2041-57. doi: 10.1105/tpc.111.095281.Kandasamy, M.K., et al. (2001). One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell. Jul;13(7):1541-54.Kandasamy, M.K., et al. (1999). The late pollen-specific actins in angiosperms. Plant Journal. Jun;18(6):681-9.
Special application note:
This antibody is a total IgG fraction, purified on a protein G column. It recognizes actin isoforms ACT2, 8, and 11.
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Sultan et al. (2017). The Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria. Plant Cell. 2016 Nov;28(11):2805-2829.Kandasamy, M.K. et al. (2012). Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell. 24, 2012 May;24(5):2041-57. doi: 10.1105/tpc.111.095281
Special application note:
This antibody is purified on a protein G column. It recognizes Arabidopsis actins ACT1, 2, 3, 4, 7, 8, 11, 12 and Dictyostelium actin
Profilin is an actin monomer-binding protein, involved in the organization and reconstruction of the actin cytoskeleton in eukaryotes. Profilin at low concentrations enhances the actin polymerization, and at high concentrations it prevents it. Porfilin 1,2 and 3 are constitutive, while profilin 4 and 5 are pollen-specific. Alternative names: PRF, PFN
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid in 0.1M PBS, pH 7.4, with 0.15M Sodium Chloride, and 0.05% Sodium Azide
Storage Temp:
Store at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Profilin is an actin monomer-binding protein, involved in the organization and reconstruction of the actin cytoskeleton in eukaryotes. Profilin also interacts with membrane polyphospho-inositides, the Arp 2/3 complex, short proline rich stretches, and other ligands.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid in 0.1M PBS, pH 7.4, with 0.15M Sodium Chloride, and 0.05% Sodium Azide
Storage Temp:
Store at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
Arabidopsis thaliana
Immunogen:
Profilin 4 (PRF4) from Arabidopsis thaliana, UniProt: Q38905, TAIR: AT4G29340
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Kandasamy, M.K., et al. (2002). Plant profilin isovariants are distinctly regulated in vegetative and reproductive tissues. Cell Motil Cytoskeleton 52(1):22-32. McKinney, E.C., et al. (2001) Small changes in the regulation of one Arabidopsis profilin isovariant, PRF1, alter seedling development. Plant Cell 13(5): 1179-91.
Special application note:
Exact working dilution needs to be determined by end user.
LacI (Lactose operon repressor) is a DNA-binding protein which represses the expression of genes encoding proteins responsible for lactose metabolism in bacteria. When lactose in absent from the bacterial medium these genes are repressed and when it becomes available it is converted to allolactose which inhibits LacI binding to DNA, thus allowing lactose metabolism.
Store at -20°C. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
Arabidopsis thaliana, Escherichia coli
Expected Species:
Actinopolyspora erythraea, Chlamydia trachomatis, Methanosarcina mazei, Methyloglobulus morosus KOM1, Pseudomonas sp. 1-7, Shigella sonnei, Shinella sp. Species of your interest not listed? Contact us
Immunogen:
Recombinant LacI-NLS-6xHis (Lactose operon repressor + C-terminal eukaryotic nuclear localization signal: SSVVHPKKKRKV (NLS) + 6xHis-tag) overexpressed in in E. coli
SAM (S-adenosylmethionin synthetase) is an enzyme which catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps. It is a donor of methyl groups in methylation reactions, and is involved in a number of processes such as: response to cadmium ions and salt stress, as well as, ethylene and lignin biosynthesis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Malus domestica
Expected Species:
Beta vulgaris, Brassica sp., Citrus sp., Coffea canephora, Caomelina sativa, Capsella rubella, Cucumis melo, Cucumis sativus, Genlisea aurea, Gentiana triflora, Guzmania wittmacki, Gossypium raimondii, Eucalyptus grandis, Eutrema salsugineum, Ipomoea batatas, Jatropha curcas, Musa acuminata, Nicotiana sp., Oryza brachyantha, Phoenix dactylifera, Populus sp., Prunus sp., Ricinus communis, Sesamum indicum, Setaria italica, Solanum pennellii, Solanum tuberosum, Spinacia oleracea, Tarenaya hassleriana, Theobroma cacao, Zea mays Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana SAM1-4, UniProt: P23686, P17562, Q9SJL8, Q9LUT2, TAIR:At1g02500, At4g01850, At3g17390, At2g36880
RpoB (RNA polymerase beta subunit (chloroplast)) is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonuceloside triphosphates as substrates.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Alloteropsis semialata, Cannabis sativa, Coleataenia prionitis, Digitaria exilis, Echinochloa crus-galli var. crus-galli , Eragrostis tef, Microlaena stipoides, Miscanthus sacchariflorus, Oryza sativa, Phragmites australis, Potamophila parviflora, Rhynchoryza subulata, Saccharum officinarum, Setaria italica, Sorghum bicolor, Stipa lipskyi, Sporobolus michauxianus, Triticum aestivum Species of your interest not listed? Contact us
Immunogen:
His-tagged, highly conserved fragment of Zea mays RpoB gi|540067377|gb|AGV02730.1| RNA polymerase beta subunit (chloroplast) [Zea mays subsp. mays], UniProt: A0A059Q6W3
RpoC1 (RNA polymerase beta' subunit (chloroplast)) is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Early light-induced proteins (ELIPs) are light stress-induced proteins related to the chlorophyll a/b binding protein family from higher plants and green algae located in the thylakoid membranes and involved in photosynthesis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Min. 30 µg of total protein has to be loaded per lane. Please note that level of Elip proteins varies considerably between different plant species and therefore protein load/well has to be adjusted.
Application Details:
1 : 500-1 : 1000 (WB)
Purity:
Affinity purified serum in PBS pH 7.4
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
AS03 036 | Anti-Elip2 | early light inducible protein 2, rabbit antibodiescollection of antibodies to proteins involved in photosynthesisPlant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Digitaria sanguinalis, Echinochloa crus-galli, Pinus strobus L.
Expected Species:
Algae, Dicots, Mosses Species of your interest not listed? Contact us
Immunogen:
BSA-conjugated synthetic peptide derived from Arabidopsis thaliana At1g29910 (Lhcb1.1), At1g29920 (Lhcb1.2), At1g29930 (Lhcb1.3, most expressed), At2g34430 (Lhcb1.4), and At2g34420 (Lhcb1.5)
This Lhcb1 antibody is directed specifically against the Arabidopsis Lhcb1 gene products, for those that would prefer higher specific activity over broader specificity offered by Agrisera older Lhcb1 antibody, AS01 004Protein is processed into mature form (Jansson 1999).
Application Details:
1 : 2500-1 : 5000 (WB)
Purity:
Affinity purified serum in PBS pH 7.4
Reconstitution:
For reconstitution add 100 µl of sterile water
Related products:
AS01 002 Anti-Lhcb3 | LHCII type III chlorophyll a/b-binding protein, rabbit antibodiesAS01 003 Anti-Lhcb2 | LHCII type II chlorophyll a/b-binding protein, rabbit antibodiesAS01 011 Set of 10 plant anti-Lhca and anti-Lhcb antibodiesLHC available antibodies against pigment-binding proteinsPlant protein extraction bufferSecondary antibodies | AgriseraSuperDeal
Molecular Weight:
28 | 25 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Wang et al. (2020). Post-translational coordination of chlorophyll biosynthesis and breakdown by BCMs maintains chlorophyll homeostasis during leaf development. Nat Commun. 2020; 11: 1254. Pralon et al. (2019). Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019 Jun 20;2:220. doi: 10.1038/s42003-019-0477-4. Lal et al. (2018). The Receptor-like Cytoplasmic Kinase BIK1 Localizes to the Nucleus and Regulates Defense Hormone Expression during Plant Innate Immunity. Cell Host Microbe. 2018 Apr 11;23(4):485-497.e5. doi: 10.1016/j.chom.2018.03.010.Tamburino et al. (2017). Chloroplast proteome response to drought stress and recovery in tomato (Solanum lycopersicum L.). BMC Plant Biol. 2017 Feb 10;17(1):40. doi: 10.1186/s12870-017-0971-0.Fristedt et al. (2017). PSB33 sustains photosystem II D1 protein under fluctuating light conditions. Journal of Experimental Botany doi:10.1093/jxb/erx218.Hartings et al. (2017). The DnaJ-Like Zinc-Finger Protein HCF222 Is Required for Thylakoid Membrane Biogenesis in Plants. Plant Physiol. 2017 Jul;174(3):1807-1824. doi: 10.1104/pp.17.00401.Correa-Galvis et al. (2016). PsbS interactions involved in the activation of energy dissipation in Arabidopsis. Nature Plants 2, Article number: 15225 (2016) doi:10.1038/nplants.2015.225Longoni et al. (2015). Phosphorylation of the Lhcb2 isoform of Light Harvesting Complex II is central to state transitions. Plant Physiol. 2015 Oct 5. pii: pp.01498.2015.Wientjes et al (2013). LHCII is an antenna of both photosystems after long-term acclimation. BBA, Jan 6.
Special application note:
A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467–9476
Profilin is an actin monomer-binding protein, involved in the organization and reconstruction of the actin cytoskeleton in eukaryotes. Profilin also interacts with membrane polyphospho-inositides, the Arp 2/3 complex, short proline rich stretches, and other ligands.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid in 0.1M PBS, pH 7.4, with 0.15M Sodium Chloride, and 0.05% Sodium Azide
Storage Temp:
Store at -20°C.Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Plant and algal protein extraction bufferSecondary antibodies
Molecular Weight:
14 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Kandasamy, M.K., et al. (2002). Plant profilin isovariants are distinctly regulated in vegetative and reproductive tissues. Cell Motil Cytoskeleton 52(1):22-32.McKinney, E.C., et al. (2001) Small changes in the regulation of one Arabidopsis profilin isovariant, PRF1, alter seedling development. Plant Cell 13(5): 1179-91.
Special application note:
Exact working dilution needs to be determined by end user.
Mouse anti-HA is a primary antibody which binds to HA and is directly conjugated to Alkaline Phosphatase . This is of advantage to shorten assay time when no need to use a secondary antibody to HA.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
The optimal working dilution should be determined by the investigator.
Purity:
Protein A purified
Related products:
AS15 2924 | Anti-HA, conjugated to HRP, mouse antibodiesAS15 2922 | Anti-HA, conjugated to Biotin, mouse antibodiesAS15 2919 | Anti-HA, conjugated to DyLight®488, mouse antibodiesAS15 2920 | Anti-HA, conjugated to DyLight® 550, mouse antibodiesAS15 2925 | Anti-HA, conjugated to DyLight® 650, mouse antibodiesAS15 2923 | Anti-HA, conjugated to Europium 1024, mouse antibodiesAS15 2918 | Anti-HA, conjugated to SureLight® R-Phycoerythrin, mouse antibodiesother tag antibodies
Selected references:
Wang et al. (2017). The inhibition of protein translation mediated by AtGCN1 is essential for cold tolerance in Arabidopsis thaliana. Plant Cell Environ. 2017 Jan;40(1):56-68. doi: 10.1111/pce.12826.
Special application note:
Antibody is provided in: 25 mM Tris, 150 mM Sodium Chloride, 5 mM Magnesium Chloride, 0.12 mM Zinc Chloride, pH 7.4 with 2 mM Sodium Azide.
AGO9 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AGO9 protein sequence UniProt: Q84YI4, TAIR:At5g21150.
Applications:
Immunolocalization, whole-mount (IL), Immunoprecipitation (IP), Western blot (WB)
AGO expression may be cell/tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Seedlings can be used as a negative control. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.
Application Details:
5 µg of antibody per 1 gram of a fresh tissue (IP), 1 : 10 000 (WB)
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Sprunck et al. (2019). Elucidating small RNA pathways in Arabidopsis thaliana egg cells. http://dx.doi.org/10.1101/525956Su et al. (2017). The THO Complex Non-Cell-Autonomously Represses Female Germline Specification through the TAS3-ARF3 Module. Curr Biol. 2017 Jun 5;27(11):1597-1609.e2. doi: 10.1016/j.cub.2017.05.021.Havecker et al. (2010) The RNA-directed DNA methylation Arabidopsis Argonautes functionally diverge based on expression and interaction with target loci. Plant Cell 22(2): 321-34.
Tubulin beta (TUB) together with alpha tubulin is making up microtubules.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
AS10 680 | Anti-tubulin alpha chain, rabbit antibodiesPlant and algal protein extraction buffer | AgriseraSuperDeal | Loading15
Molecular Weight:
49 | 49 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113
Special application note:
Please, note that tubulin beta is only detected in actively dividing meristematic cells (see immunolocalization image below). Therefore to allow detection on a western blot, analyzed material must contain enough meristematic cells with dividing activity.Signal in a western blot application in Chlamydomonas reinhardtii is obtained with a load of 100 µg/well and a dilution of 1: 500.
Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Amborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandis, Glycine max, Chlorella variabilis, Leucaena glauca, Lotus japonicus, Medicago tribuloides, Mimulus guttatus, Musa malaccensis, Oryza sativa, Panicum italicum, Physcomitrella patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Populus trichocarpa, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530
Applications:
Immunolocalization (IL), Immunofluorescence (IF), Western blot (WB)
Ranjan et al. (2019). Transient Internalization and Microtubule-Dependent Trafficking of a Ciliary Signaling Receptor from the Plasma Membrane to the Cilium. Curr Biol. 2019 Aug 2. pii: S0960-9822(19)30867-X. doi: 10.1016/j.cub.2019.07.022.Wattelet-Boyer et al. (2016). Enrichment of hydroxylated C24- and C26-acyl- chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains. Nat Commun. 2016 Sep 29;7:12788. doi: 10.1038/ncomms12788.Derbyshire et al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314.Grones et al. (2015). Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. J Exp Bot. 2015 Apr 28. pii: erv177.McLoughlin et al. (2013). Identification of novel candidate phosphatidic acid binding proteins involved in the salt stress response of Arabidopsis thaliana roots. Biochem J. Jan 17.
RCD1 (Radical cell death 1) functions as a regulator of oxidative-stress hormonal and developmental responses. Alternative name: Inactive poly [ADP-ribose] polymerase RCD1.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RCD1 protein sequence UniProt: Q8RY59, TAIR: AT1G32230
Applications:
Western blot (WB) following immunoprecipitation (IP)
Protein has three splice variants: 63.8 + 65.6 + 65.7 kDPeptide used to elicit this antibody was designed for detection of all (3) RCD1 splice variants but NOT the close relative SRO1.
Application Details:
1 : 1000 (WB) on samples following IP
Purity:
Affinity purified serum in PBS, pH 7.4
Reconstitution:
For reconstitution add 50 µl of sterile water.
Related products:
collection of antibodies to proteins involved in oxidative stress Plant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
65 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
HDT1 (HD2A) is probably mediating in the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in cell cycle progression, transcriptional regulation and developmental events. This protein is also involved in rRNA gene silencing in nucleolar dominance. Synonymes:HD-tuins protein 1, Histone deacetylase 2a.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Expected Species:
Arabidopsis thaliana
Immunogen:
Recombinant part of Arabidopsis thaliana HDT1 Q9FVE6, At3g44750
AS11 1753 | histone deacetylase| HDT3collection of antibodies for developmental biologyPlant protein extraction buffer Secondary antibodies
Molecular Weight:
26.4 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Derbyshire et al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314.
Special application note:
Antibody is present in PBS + 50 % glycerol and 0.01 % of sodium azide as preservative of bacterial growth
Aleu protein may play a role in proteolysis leading to mobilization of nitrogen during senescence and starvation. Catalytic activity : Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-|-Xaa bonds) as well as an endopeptidase. Cellular localisation: vacuole. Alternative names: Senescence-associated gene product 2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier protein.
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica oleracea, Camelina sativa, Capsella rubella, Eutrema salsugineum, Raphanus sativus Species of your interest not listed? Contact us
Immunogen:
Recombinant, His6-tagged, ALEU protein from Arabidopsis thaliana, UniProt: Q8H166, TAIR: At5g60360
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Takagi et al. et al. (2013). MAIGO5 functions in protein export from Golgi-associated endoplasmic reticulum exit sites in Arabidopsis. Plant Cell. 2013 Nov;25(11):4658-75. doi: 10.1105/tpc.113.118158. (Western blot, Arabidopsis thaliana)Ueda et al. (2006). AtVAM3 is required for normal specification of idioblasts, myrosin cells. Plant Cell Physiol. 2006 Jan;47(1):164-75. doi: 10.1093/pcp/pci232. (Western blot, Arabidopsis thaliana)
DEG15 is a peroxisomal Deg-protease with endopeptidase activity. This protease cleaves specifically substrates with Cys in the P1 and P2 position and acts as peroxisomal processing peptidase. Alternative names: At1g28320/F3H9_2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Micromonas sp., Oryza sativa, Populus balsamifera, Solanum lycopersicum, Sorghum vulgare, Ricinus communis, Vitis vinifera, Zea mays Species of your interest not listed? Contact us
Immunogen:
synthetic peptide derived from Arabidopsis thaliana DEG15, Q8VZD4 (At1g28320)
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Schuhmann et al. (2008). The DEG15 serine protease cleaves peroxisomal targeting signal 2-containing proteins in Arabidopsis.Plant Physiol. 4: 1847-1856.Helm et al. (2007). Dual specificities of the glyoxysomal/peroxisomal processing protease Deg15 in higher plants.PNAS 27: 11501-11506.
Vacuolar-sorting receptor (VSR) involved in clathrin-coated vesicles sorting from Golgi apparatus to vacuoles. Required for the sorting of 12S globulin, 2S albumin and maybe other seed storage proteins to protein storage vacuoles (PSVs) in seeds. May also be implicated in targeting N-terminal propeptide containing proteins to lytic vacuoles. Subcellular localisation: Golgi apparatus membrane.Alternative names: BP80-like protein b, Epidermal growth factor receptor-like protein 1, AtELP, AtELP1, Spot 3 protein
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier protein.
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Nicotiana tabacum
Expected Species:
Brassica rapa, Capsella rubella, Camelina sativa, Eutrema salsugineum, Raphanus sativus, Tarenaya hassleriana Species of your interest not listed? Contact us
Immunogen:
Recombinant, His6-tagged, VSR1 from Arabidopsis thaliana, UniProt: P93026, TAIR: At3g52850
Higher apparent molecular weight of VSR1 is due to three determined glycosylations and several more predicted.
Application Details:
1: 500 (IF), 1: 1000-1: 5000 (WB)
Purity:
Total IgG, purified on Protein A
Related products:
collection of antibodies to membrane transport system
Molecular Weight:
68 | 80 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Fuji et al. (2016). The Adaptor Complex AP-4 Regulates Vacuolar Protein Sorting at the trans-Golgi Network by Interacting with VACUOLAR SORTING RECEPTOR1. Plant Physiol. 2016 Jan;170(1):211-9. doi: 10.1104/pp.15.00869. (Western blot, Arabidopsis thaliana) Kunieda et al. (2013). Spatiotemporal secretion of PEROXIDASE36 is required for seed coat mucilage extrusion in Arabidopsis. Plant Cell. 2013 Apr;25(4):1355-67. doi: 10.1105/tpc.113.110072. (Western blot, Arabidopsis thaliana)Yamada et al. (2005). Endosomal proteases facilitate the fusion of endosomes with vacuoles at the final step of the endocytotic pathway. Plant J. 2005 Mar;41(6):888-98. doi: 10.1111/j.1365-313X.2005.02349.x. (Immunofluorescence, Nicotiana tabacum)
Delta VPE (Vacuplar-Processing Enzyme delta-isozyme) is Asparagine specific endopeptidase that may be involved in processing of proteins targeted to vacuoles (By similarity). The enzyme is probably involved in post-translational proteolysis of seed storage proteins in the protein storage vacuole of developing seeds (PubMed:12417707, PubMed:14688293). Exhibits a caspase-1-like activity in extracellular granules. At the early stage of seed development, required for the formation of the seed coat, by regulating cell death of specific cell layers in inner integument (Nakaune et al. 2005).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier protein.
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Camelina sativa, Capsella rubella, Eutrema salsugineum, Raphanus sativus Species of your interest not listed? Contact us
Immunogen:
Recombinant, His6-tagged, delta-VPE from Arabidopsis thaliana, UniProt: Q9LJX8, TAIR: At3g20210
Applications:
Immunofluorescence (IF), Immunolocalisation (IL), Western blot (WB)
Subcellular localisation is seed specific and restricted to developing seeds at 7 days after anthesis. Delta-VPE is detected at lower levels in flowers siliques, specifically in seed coats.
Application Details:
1: 500 (IF), 1: 5000 (IL), 1: 5000 (WB)
Purity:
Total IgG, purified on Protein A
Related products:
collection of antibodies to membrane transport system
Molecular Weight:
52 kDa (inactive precursor) | 37-38 kDa (mature, active form)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Kunieda et al. (2013). Spatiotemporal secretion of PEROXIDASE36 is required for seed coat mucilage extrusion in Arabidopsis. Plant Cell . 2013 Apr;25(4):1355-67.doi: 10.1105/tpc.113.110072. (Western blot, Arabidopsis thaliana)Kunieda et al. (2008). NAC family proteins NARS1/NAC2 and NARS2/NAM in the outer integument regulate embryogenesis in Arabidopsis. Plant Cell. 2008 Oct;20(10):2631-42.doi: 10.1105/tpc.108.060160. (Western blot, Arabidopsis thaliana)
PYK10 is the main component of ER bodies. It has hydrolase activity, hydrolyzing O-glycosyl compounds It may produce defense compounds when plants are damaged by insects or wounding.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier protein.
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Conjugated peptide, derived from Arabidopsis thaliana internal part of PYK10, UniProt: A0A178VCN3, TAIR: At3g08880.
Applications:
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Yamada et al. (2013). Identification of Two Novel Endoplasmic Reticulum Body-Specific Integral Membrane Proteins. Plant Physiol. 2013 Jan;161(1):108-20. doi: 10.1104/pp.112.207654. (Western blot, Arabidopsis thaliana) Nagano et al. (2005). Activation of an ER-body-localized ß-Glucosidase via a Cytosolic Binding Partner in Damaged Tissues of Arabidopsis thaliana. Plant Cell Physiol. 2005 Jul;46(7):1140-8. doi: 10.1093/pcp/pci126. (Immunoprecipiation, Western blot, Arabidopsis thaliana)Matshushima et al. (2003). A novel ER-derived compartment, the ER body, selectively accumulates a beta-glucosidase with an ER-retention signal in Arabidopsis. Plant J . 2003 Feb;33(3):493-502. doi: 10.1046/j.1365-313x.2003.01636.x. Immunofluorescence, Immunohistochemistry, Western blot, Arabidopsis thaliana)
PYK10 is the main component of ER bodies. It has hydrolase activity, hydrolyzing O-glycosyl compounds It may produce defense compounds when plants are damaged by insects or wounding. Cellular localisation: ER bodies.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier protein.
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Conjugated peptide, derived from Arabidopsis thaliana C-terminal of PYK10, UniProt: A0A178VCN3, TAIR: At3g08880.
N-terminal signal peptide including 24 amino acis and ER retention signal is removed from the mature protein.
Application Details:
1:500-1:1000 (IHC), 1: 5000- 1: 20 000 (WB)
Purity:
Total IgG, purified on Protein A
Related products:
endomembrane system antibody collection
Molecular Weight:
59.7 | 56 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Matsushima et al. (2003). A novel ER-derived compartment, the ER body, selectively accumulates a beta-glucosidase with an ER-retention signal in Arabidopsis. Plant J. 2003 Feb;33(3):493-502. doi: 10.1046/j.1365-313x.2003.01636.x.
SMT1 (EC=2.1.1.41) is an enzyme involved in steroid biosynthesis localized specific to the Endoplasmic Reticulum (ER), Boutte et al., 2009 . It is catalyzing the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol. The protein is highly expressed in vascular tissue, mature leaves and in regions undergoing cellular expansion. Alternative names: Cycloartenol-C-24-methyltransferase, 24-sterol C-methyltransferase 1, Protein STEROL METHYLTRANSFERASE 1, Protein CEPHALOPOD
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Amborella trichopoda, Brassica napus, Brassica rapa, Capsella rubella, Citrus clementina, Eutrema salsugineum, Glycine max, Glycine soja, Gossypium mexicanum, Medicago truncatula, Populus trichopocarpa, Prunus persica, Ricinus communis, Theobroma cacao, Vitis vinifera Species of your interest not listed? Contact us
SMT1 antibody characterization in Western Blot and immunofluorescence labeling: Boutté Y et al. (2010). Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis. EMBO J. 29, 5465-5458.
Application Details:
1 : 50-1 : 100 (IL), 1 : 500-1 : 1000 (WB)
Purity:
Affinity purified IgG
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
AS09 481 | Anti-BiP2 | luminal-binding protein 2, rabbit antibodiesAntibodies to other proteins of endomembrane system | AgriseraSuperDeal
Collins et al. (2020). EPSIN1 Modulates the Plasma Membrane Abundance of FLAGELLIN SENSING2 for Effective Immune Responses . Plant Physiol. 2020 Feb 24. pii: pp.01172.2019. doi: 10.1104/pp.19.01172Yang et al. (2016). Arabidopsis PROTEASOME REGULATOR1 is required for auxin-mediated suppression of proteasome activity and regulates auxin signalling. Nat Commun. 2016 Apr 25;7:11388. doi: 10.1038/ncomms11388.LaMontagne et al. (2016). Isolation of Microsomal Membrane Proteins from Arabidopsis thaliana. Curr. Protoc. Plant Biol. 1:217-234. doi: 10.1002/cppb.20020.Yoshimoto et al. (2014). Quality control of plant peroxisomes in organ specific manner via autophagy. J cell Science, August 1, 2014, 127 (15).
Special application note:
SMT1 is an integral membrane protein of ER (Boutte et al., 2009) while BiP is a membrane associated protein.Endogenous SMT1 is expressed at very low levels in root epidermis and root cap as compare to cortex and endodermis and therefore this can contribute to SMT1 detection problems.
Caleosin3 encodes a calcium binding protein whose mRNA is induced upon treatment with NaCl, ABA and in response to desiccation. mRNA expression under drought conditions is apparent particularly in leaves and flowers. Isoform of caleosin with a role as a peroxygenase involved in oxylipin metabolism during biotic and abiotic stress. Involved in the production of 2-hydroxyoctadecatrienoic acid. The peroxygenase has a narrow substrate specificity thus acting as a fatty acid hydroperoxide reductase in vivo. Protective role to fungus pathogen has been indicaed. Expression is very low in young leaves and high in senescent leaves.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier protein.
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus, Camelina sativa, Capsella rubella, Raphanus sativus Species of your interest not listed? Contact us
Immunogen:
BSA-conjugated peptide, derived from N-terminus of Arabidopsis thaliana Caleosin 3, UniProt: O22788, TAIR: At2g33380
collection of antibodies to proteins involved in drought response
Molecular Weight:
26.6 | 30 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Shimada et al. (2014). Leaf oil body functions as a subcellular factory for the production of a phytoalexin in Arabidopsis. Plant Physiol. 2014 Jan;164(1):105-18. doi: 10.1104/pp.113.230185.Shimada et al. (2010). A rapid and non-destructive screenable marker, FAST, for identifying transformed seeds of Arabidopsis thaliana. Plant J. 2010 Feb 1;61(3):519-28. doi: 10.1111/j.1365-313X.2009.04060.x
UCP (uncoupling protein) is an inner membrane mitochondrial protein that can dissipate the proton gradien before it can be used to provide the energy for oxidative phosphorylation. Synonymes: AtUCP, Uncoupling protein 2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). Alternative name: AtBP2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Piea sitchensis, Populus trichocarpa, Spinacia oleracea, Zea mays Species of your interest not listed? Contact us
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Application Details:
1 : 50-1 : 1000 (IF), 1 : 2000 (WB)
Purity:
Affinity purified IgY in PBS pH 7.4
Related products:
AS09 481 | Anti-BiP2 | lumenal-binding protein 2, rabbit antibodiesAS09 615 | Anti-BiP2 | lumenal-binding protein 2, goat antibodiesantibodies to plant endomembrane system proteins Plant protein extraction bufferSecondary antibodies
Molecular Weight:
73.5 | 80 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Bennett et al. (2014). Plasma Membrane-Targeted PIN Proteins Drive Shoot Development in a Moss. Curr Biol. 2014 Dec 1;24(23):2776-85. doi: 10.1016/j.cub.2014.09.054. Epub 2014 Nov 13.
Special application note:
Antibody solution contains 0.02% sodium azide as preservative.
MEB1 (Membrane protein of ER body 1) displays iron ion transmembrane transporter activity and may sequester excess cytosolic iron and manganese into endoplasmic reticulum to reduce metal ion toxicity. Not essential for the accumulation of ER body components.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
Purified recombinant MEB1 of Arabidopsis thaliana, residues 271-502 with a His tag, UniProt: Q8W4P8, TAIR: AT4G27860
Applications:
ELISA (ELISA), Immunoprecipitation (IP), Western blot (WB)
BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). Alternative name: AtBP2
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Picea sitchensis, Populus trichocarpa, Physcomitrella patens, Spinacia oleracea, Zea mays Species of your interest not listed? Contact us
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Antibody has a reduced reactivity to monocots in western blot.
Application Details:
1 : 2000 (WB)
Purity:
Affinity purified serum in PBS, pH 7.4
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
AS09 614 | Anti-BiP2 | lumenal-binding protein, 2 chicken antibodiesAS09 481 | Anti-BiP2 | lumenal-binding protein 2, rabbit antibodiesantibodies to plant endomembrane system proteins Plant protein extraction bufferSecondary antibodies
Molecular Weight:
73.5 | 80 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Narusaka et al (2016). Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1. Sci Rep. 2016 Jan 11;6:18702. doi: 10.1038/srep18702.
Glutamine synthetase plays a role in the flow of nitrogen into nitrogenous organic compounds. There are five root types of GS isoproteis (GS1-1~ GS1-5) and one chloroplastic GS isoprotein (GS2/GLN2) are known. The sequence identity between GS1-1 and GS2 is 65%.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Spinacia oleracea, Zea mays
Expected Species:
Brachypodium distachyon, Cucurbita moschata, Glycine max, Medicago truncatula, Oryza sativa, Panicum hallii, Pontederia crassipes, Populus tremula x Populus alba, Prunus persica, Raphanus sativus, Saccharum officinarum, Setaria italica, Sorghum bicolor, Triticum aestivumSpecies of your interest not listed? Contact us
Immunogen:
Purified full length, tag cleaved, recombinant Zea mays GS-1 (Glutamine synthetase, root isozyme 1) UniProt: P38559
No confirmed exceptions from predicted reactivity are currently known.
Application Details:
assay dependent (ELISA), 1: 1000 - 1: 5000 (WB)
Purity:
Total IgG, purified on Protein A/G mix
Related products:
collection of antibodies to proteins involved in nitrogen metabolism
Molecular Weight:
39 kDa | 43 kDa (chloroplast isoform)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Kimata-Ariga and Hase (2014). Multiple complexes of nitrogen assimilatory enzymes in spinach chloroplasts: possible mechanisms for the regulation of enzyme function. PLoS One . 2014 Oct 1;9(10):e108965. doi: 10.1371/journal.pone.0108965. Sakaibara et al. (1996). Molecular identification and characterization of cytosolic isoforms of glutamine synthetase in maize roots. J Biol Chem. 1996 Nov 22;271(47):29561-8. doi: 10.1074/jbc.271.47.29561.
Special application note:
This antibody reactis with all glutamine synthetase isotypes from leaf and root.
Glutamine oxoglutarate aminotransferase (GOGAT) is an enzyme involved in synthesis of glutamate from glutamine and alpha-ketoglutarate. GOGAT has two forms in plants: ferredoxin-dependent GOGAT (Fd-GOGAT) and NADH-dependent GOGAT (NADH-GOGAT). 95% of GOGAT found in plants is the Fd-GOGAT type. Fd-GOGAT is encoded by two genes, glu1 and glu2 in Arabidopsis. Fd-GOGAT (both forms) is highly conserved among plants, red algae, and cyanobacteria. Ferredoxin-dependent glutamate synthase, chloroplastic (Fd-GOGAT) is involved in glutamate biosynthesis in leaf. This protein required for the reassimilation of ammonium ions generated during photorespiration. Gene name is GlsF.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Ariga and Hase (2014). Multiple complexes of nitrogen assimilatory enzymes in spinach chloroplasts: possible mechanisms for the regulation of enzyme function. PLoS One. Oct 1;9(10):e108965. doi: 10.1371/journal.pone.0108965.Sakaibara et al. (1991). Molecular cloning and characterization of complementary DNA encoding for ferredoxin-dependent glutamate synthase in maize leaf. J Biol Chem. Feb 5;266(4):2028-35.
Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Higher plants also possess genes for significantly different, as yet uncharacterized Fd proteins, with extended C termini (FdCs). Whether these FdC proteins function as photosynthetic electron transfer proteins is not known. It has been suggested that FdC1 has a specific function in conditions of acceptor limitation at PSI, and channels electrons away from NADP(+) photoreduction.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
Brassica rapa, Cannabis sativa, Theobroma cacao Species of your interest not listed? Contact us
Immunogen:
Purified full length, tag cleaved, recombinant Arabidopsis thaliana Ferredoxin C-1, UniProt: O23344 , TAIR: At4g14890
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Voss et al. (2011). FdC1, a Novel Ferredoxin Protein Capable of Alternative Electron Partitioning, Increases in Conditions of Acceptor Limitation at Photosystem I. J Biol Chem. 2011 Jan 7;286(1):50-9. doi: 10.1074/jbc.M110.161562.
Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Occupies a key position both for transferring the photoreducing power to Fd-NADP+ oxidoreductase (FNR), hence the formation of NADPH, and for mediating the cyclic electron flow around photosystem I (PSI).
Product Type:
Antibody
Antibody Type:
Polyclonal
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
plant ferredoxin isoformsSpecies of your interest not listed? Contact us
Immunogen:
Native, chromafography purified ferredoxin mixture: Fd1, Fd2, Fd3 and Fd4 from Zea mays, UniProt: P27787 (Fd1), P27787(Fd2), P27788 (Fd3), accesion number not known for Fd4
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Hase et al. (1991). Molecular Cloning and Differential Expression of the Maize Ferredoxin Gene Family. Plant Physiol. 96(1):77-83. doi: 10.1104/pp.96.1.77.
Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Fd3 is non-photosynthetic Fd expressed more in root than in leaf. Fd3 is localised in chloroplast and plastids.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 1 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
Brachypodium distachyon, Dichanthelium oligosanthes, Hordeum vulgare, Oryza sativa, Panicum hallii, Saccharum sp. , Setaria italicaSpecies of your interest not listed? Contact us
Immunogen:
Purified full length, tag cleaved, recombinant maize Fd3, UniProt: P27788
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Hanke and Hase (2008). Variable Photosynthetic Roles of Two Leaf-Type Ferredoxins in Arabidopsis, as Revealed by RNA Interference. Photochem Photobiol. 84(6):1302-9. doi: 10.1111/j.1751-1097.2008.00411.x. Hanke et al. (2003). A Post Genomic Characterization of Arabidopsis Ferredoxins. Plant Physiol. 134(1):255-64. doi: 10.1104/pp.103.032755. Matsumura et al. (1997). A Nitrate-Inducible Ferredoxin in Maize Roots. Genomic Organization and Differential Expression of Two Nonphotosynthetic Ferredoxin Isoproteins. Plant Physiol. 114(2):653-60. doi: 10.1104/pp.114.2.653.
Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. Occupies a key position both for transferring the photoreducing power to Fd-NADP+ oxidoreductase (FNR), hence the formation of NADPH, and for mediating the cyclic electron flow around photosystem I (PSI). Fd2 is most abundant Fd isoprotein expressed in plant leaves.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 1 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
AS20 4434 | Anti-Fd1 | Ferredoxin 1 (chloroplastic), rabbit antibodiescollection of antibodies involved in photosynthesis
Molecular Weight:
15 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Ramirez et al. (2013). Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiency.Hanke et al. (2004). A post genomic characterizationof Arabidopsis ferredoxins. Plant Physiol. 2004 Jan;134(1):255-64. Epub 2003 Dec 18 (Western blot, Arabidopsis thaliana)
Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. It occupies a key position both for transferring the photoreducing power to Fd-NADP+ oxidoreductase (FNR), hence the formation of NADPH, and for mediating the cyclic electron flow around photosystem I (PSI).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 1 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
Brassica napus, Brassica oleracea, Eutrema salsugineum, Raphanus sativusSpecies of your interest not listed? Contact us
Immunogen:
Purified full length, tag cleaved, recombinant maize Fd1, UniProt: P27787
collection of antibodies involved in photosynthesis
Molecular Weight:
12 | 15 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Hanke and Hase (2008). Variable Photosynthetic Roles of Two Leaf-Type Ferredoxins in Arabidopsis, as Revealed by RNA Interference. Photochem Photobiol. 84(6):1302-9. doi: 10.1111/j.1751-1097.2008.00411.x.Kimata and Hase (1989). Localization of Ferredoxin Isoproteins in Mesophyll and Bundle Sheath Cells in Maize Leaf. Plant Physiol. 89(4):1193-7. doi: 10.1104/pp.89.4.1193.
ABI5 (abscisic acid insensitive 5) is involved in ABA-regulated gene expression during seed development and subsequent vegetative stage and acts as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Alternative names: ABI5, ABA INSENSITIVE 5, GIA1, GROWTH-INSENSITIVITY TO ABA 1, Dc3 promoter-binding factor 1, AtDPBF1, GROWTH-INSENSITIVITY TO ABA 1, bZIP transcription factor 39, AtbZIP39.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica napus, Populus trichocarpa Species of your interest not listed? Contact us
ABI5 protein is present in very low levels therefore specific material should be used for analysis as well as chemiluminescence detection reagent in extreme low femtogram range, as AgriseraECLSuperBright.
Application Details:
1: 140 (IL), 1 : 200 (WB)
Purity:
Affinity purified serum in PBS, pH 7.4
Reconstitution:
For reconstitution add 100 µl of sterile water to each tube.
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
MG132 is recommened to be added to extraction buffer as ABI5 is degraded by proteasome as well as homogenization with thiourea and bead beater. Protocol for protein extraction from seeds can be requested here.
Ferredoxin-NADP reductase (FNR) isoproteins of plant roots play a key role in redox homeostasis of NADPH / NADP + and donation of reducing equivalence to ferredoxin. R-FNR2 is major form of R-FNR and involved in reduction and detoxication of nitrite in root.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 1 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Antibody will recognize two root FNR proteins (R-FNR1 and R-FNR2) of Arabidopsis thaliana and Zea mays as well as leaf FNRs.This antibody can be used as a marker of plastids localised in roots.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Hachiya et al. (2016). Arabidopsis Root-Type Ferredoxin:NADP(H) Oxidoreductase 2 Is Involved in Detoxification of Nitrite in Roots . Plant Cell Physiol. 57(11):2440-2450. doi: 10.1093/pcp/pcw158.Hachiya et al. (2016). Arabidopsis Root-Type Ferredoxin:NADP(H) Oxidoreductase 2 Is Involved in Detoxification of Nitrite in Roots. Plant Cell Physiol. 57(11):2440-2450. doi: 10.1093/pcp/pcw158.Onda et al. (2000). Differential Interaction of Maize Root ferredoxin:NADP(+) Oxidoreductase With Photosynthetic and Non-Photosynthetic Ferredoxin Isoproteins. Plant Physiol. 123(3):1037-45. doi: 10.1104/pp.123.3.1037. Onda et al. (2000). Differential Interaction of Maize Root ferredoxin:NADP(+) Oxidoreductase With Photosynthetic and Non-Photosynthetic Ferredoxin Isoproteins. Plant Physiol. 123(3):1037-45. doi: 10.1104/pp.123.3.1037.
FNR3 | Ferredoxin NADP Reductase, isoprotein 3 (leaf) (L-FNR1) plays a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power. Localized in chloroplast.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 1 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
Dichanthelium oligosanthes, Panicum hallii, Sorghum bicolor Species of your interest not listed? Contact us
Immunogen:
Purified full length, tag cleaved, recombinant maize leaf FNR3, UniProt: B4FUM2
This antibody is also detecting other maize L-FNRs: FNR2 and FNR1 in Zea mays and Arabidopsis thaliana leaf extracts, in the order of reactivity in each species.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Okutani et al. (2005). Three Maize Leaf ferredoxin:NADPH Oxidoreductases Vary in Subchloroplast Location, Expression, and Interaction With Ferredoxin. Plant Physiol . 2005 Nov;139(3):1451-9. doi: 10.1104/pp.105.070813.Okutani et al. (2005). Three Maize Leaf ferredoxin:NADPH Oxidoreductases Vary in Subchloroplast Location, Expression, and Interaction With Ferredoxin. Plant Physiol. 2005 Nov;139(3):1451-9. doi: 10.1104/pp.105.070813.
FNR2 | Ferredoxin NADP Reductase, isoprotein 2 (leaf) plays a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power.Alternative names: FNR
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 1 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Storage Temp:
Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Zea mays
Expected Species:
Dichanthelium oligosanthes, Sorghum bicolor Species of your interest not listed? Contact us
Immunogen:
Purified full length, tag cleaved, recombinant maize leaf FNR2, UniProt: Q9SLP5, sharing homology with Arabidopsis thaliana FNR2, UniProt: Q8W493
Assimilatory nitrate reductase (NR), (EC.1.6.6.1) catalyses the reduction of nitrate to nitrite in the cytoplasm. Plants contain 2 forms of NR: NADH-NR (most common form in plants and algae, predominantly found in green tissues) and NAD(P)H-NR (uses NADH or NADPH as the electron donor, constitutively expressed in plants at a low level). NADH-NR is a homodimer of two identical subunits (100-115 kDa each, hold together by a Mo-cofactor) each of them coded by up to three genes (NR1-3, NIA1-NIA3).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
KLH-conjugated synthetic peptide derived from conserved domain in NADH-NR protein sequences including A.thaliana NR1 P11832, At1g77760 and NR2 P11035, At1g37130
ECL based detection systems are adviced to use since to low signal intensity can be obtaied with BCIP/NBT system.In Chlamydmonas reinhardtii anti-NR antibody is also reacting with L-Aminoacid Oxidase (a nitrogen scavenging enzyme induced during nitrogen starvation).
Zhang et al. (2020). Hydrogen sulfide and rhizobia synergistically regulate nitrogen (N) assimilation and remobilization during N deficiency-induced senescence in soybean. Plant Cell Environ. 2020 Feb 3. doi: 10.1111/pce.13736.Dongxu et al. (2020). Magnesium reduces cadmium accumulation by decreasing the nitrate reductase-mediated nitric oxide production in Panax notoginseng roots. Journal of Plant Physiology. Available online 7 February 2020, 153131Jayawardena et al. (2016). Elevated CO2 plus chronic warming reduces nitrogen uptake and levels or activities of nitrogen -uptake and -assimilatory proteins in tomato roots. Physiol Plant. 2016 Nov 28. doi: 10.1111/ppl.12532. [Epub ahead of print]Chen et al. (2016). The role of nitric oxide signalling in response to salt stress in Chlamydomonas reinhardtii. Planta. 2016 Sep;244(3):651-69. doi: 10.1007/s00425-016-2528-0. Epub 2016 Apr 26.Cheng et al. (2015). Quantitative proteomics analysis reveals that S-nitrosoglutathione reductase (GSNOR) and nitric oxide signaling enhance poplar defense against chilling stress. Planta. 2015 Aug 2.Zhang et al. (2014). Heterologous expression of AtPAP2 in transgenic potato influences carbon metabolism and tuber development. FEBS Lett. 2014 Aug 27. pii: S0014-5793(14)00621-8. doi: 10.1016/j.febslet.2014.08.019.Beyzaei et al. (2014). Response of Nitrate Reductase to Exogenous Application of 5-Aminolevulinic Acid in Barley Plants. J. Plant Growth Regulation, April 2014.Frada et al. (2013). Quantum requirements for growth and fatty acid biosynthesis in the marine diatom Phaeodactylum tricornutum (Bacilloriophyceae) in nitrogen replete and limited conditions. J. Phycology. Diatom growth and lipid efficiency
Tubulin gamma chain is essential for the control of microtubular network. It is found at microtubule organizing centers (MTOC) such as the spindle poles
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at 4°C; Do not freeze. Do not exceed exipry date is provided on the tube. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
human, protozoa
Expected Species:
PlantsSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449. UniProt: P23258The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.
This antibody recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope which this antibody is recognizing is conserved in Arabidopsis thaliana Tubulin gamma-1 chain, UniProt: P38557, Gene ID: At3g61650 and Tubulin gamma-2 chain, UniProt: P38558, Gene ID: At5g05620Recommended secondary antibody: goat anti-mouse IgG1 AS16 3715
Clone number:
IgG1
Application Details:
2-8 µg/ml (ICC), 1 µg/ml (WB)
Purity:
Purified by Protein A affinity chromatography in PBS with 15 mM sodium azide.
Related products:
Collection of antibodies to cytoskeleton proteinsAS10 681 | Anti-tubulin beta chain, rabbit antibodies
Molecular Weight:
51 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Together with gamma –glutamyl Cys sythetase (γ-ECS) and glutathione synthetase, phytochelatin synthetase (PS), is an enzyme involved in, and required for, synthetizing phytochelatins (PC). In certain plants, deficiency of such enzymes leads to low tolerance to metal contamination while mutants over-expressing them are highly tolerant to metal contamination and may be used in phyto-remediation of heavy metals.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Protein G purified immunoglobulin in PBS pH 7.4, 150 mM NaCl, 0.05 % sodium azide
Storage Temp:
Store at -20°C. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
Schizosaccharomyces pombe
Immunogen:
N-terminal peptide conjugated to MAP (Multiple Antigenc Peptide)
Applications:
ELISA (ELISA), Immunoflourescence (IF), Western blot (WB)
This antibody recognized yeast PS overexpressed in Arabidopsis thaliana.
Clone number:
IgG2a
Application Details:
1: 1000 (ELISA), (WB)
Related products:
collection of antibodies to heavy metal stress
Molecular Weight:
57 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity known at the moment.
Selected references:
Chen et al (2005). Overexpression of phytochelatin synthase in Arabidopsis leads to enhanced arsenic tolerance and cadmium hypersensitivity. Plant Cell Physiol. 2004 Dec;45(12):1787-97. Erratum in: Plant Cell Physiol. 46(2):387.
Glutamate dehydrogenase 1 (EC=1.4.1.3) is an enzyme localized in mitochondrial matrix which plays a key role in nitrogen and glutamate metabolism.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
AS16 4034 | Anti-GDH2 | Glutamate dehydrogenase 2, rabbit antibodiesAntibodies to various proteins involved in nitrogen metabolismPlant protein extraction buffer
Molecular Weight:
44 kDa
Not reactive in:
Algae
Selected references:
Sarasketa et al. (2014). Exploring ammonium tolerance in a large panel of Arabidopsis thaliana natural accessions. J Exp Bot. 2014 Sep 9. pii: eru342.Tsilikochrisos et al. (2014). Glutamate dehydrogenase is differentially regulated in seeded and parthenocarpic tomato fruits during crop development and postharvest storage. Scientia Horticulturae 181 (2015) 34–42. (immunolocalization)
CAH3 - carbonic anhydrase is a zinc-containing metalloenzyme that catalyzes the reversible interconversion of CO2 and HCO3?. It plays an important role in many physiological functions that involve decarboxylation or carboxylation reactions, including both photosynthesis and respiration.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Chlamydomonas reinhardtii
Expected Species:
Chlamydomonas reinhardtii (only)
Immunogen:
Recombinant carbonic anhydrase of Chlamydomonas reinhardtii A8J4Z8 after cleavage of a fusion protein
Applications:
immunofluorescence (IF), Immunogold (IG), Western blot (WB)
AS11 1737 | Anti-Beta-CA1, beta-CA2 | carbonic anhydrase | mitochondrial | Chlamydomonas, rabbit antibodiesantibodies to other photosynthetic proteinsCollection of antibodies to Chlamydomonas proteinsAlgal protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
33.4 | 29 kDa
Not reactive in:
Arabidopsis thaliana, Oryza sativa
Selected references:
Terentyev (2020: The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated From Wild Type and cia3 Mutant Chlamydomonas reinhardtii. Life (Basel). 2020 May 14;10(5):E63. doi: 10.3390/life10050063..Terentyev et al. (2019). Carbonic anhydrase CAH3 supports the activity of photosystem II under increased pH. Biochim Biophys Acta Bioenerg. 2019 Jul 1;1860(7):582-590. doi: 10.1016/j.bbabio.2019.06.003.Correa-Galvis et al. (2016). Photosystem II Subunit PsbS Is Involved in the Induction of LHCSR Protein-dependent Energy Dissipation in Chlamydomonas reinhardtii. J Biol Chem. 2016 Aug 12;291(33):17478-87. doi: 10.1074/jbc.M116.737312.Mitchell et al. (2014). Dynamics of carbon concentrating mechanism induction and protein re-localisation during the dark to light transition in synchronised Chlamydomonas. Plant Physiol. 2014 Aug 8. pii: pp.114.246918.Tirumani et al. (2014). Regulation of CCM genes in Chlamydomonas reinhardtii during conditions of light-dark cycles in synchronous cultures. Plant Mol Biol. 2014 Mar 4.Shutova et al. (2008). The photosystem II-associated Cah3 in Chlamydomonas enhances the O2evolution rate by proton removal. EMBO J. 27:782-791.
Special application note:
This antibody recognizes native and recombinant carbonic anhydrase of Chlamydomonas reinhardtii.
AtPCaP1 is a protein associated with plasma membrane with ability to bind Ca, calmodulin and phosphatidylinositol phoshphates. Alternative names: At4g20260, endomembrane-associated protein.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Raphanus sativus (41 kDa), Brassica rapa (42 kDa), Brassica rapa var. glabra Regel (43 kDa), Brassica oleracea var. italica (41 kDa)
Expected Species:
Brassicaceae Species of your interest not listed? Contact us
It is confirmed that the nock-out mutant (SALK_022955, Col-0 background) has no mRNA or protein of PCaP1. In the immunoblot, crude membrane fractions prepared from wild-type and pcap1 knock-out mutant plants were examined. Therefore the antibody to PCaP1 specifically recognizes PCaP1 and can be used for immunoblotting. For application in immunocytochemistry cross reacting band in proximity of Rubisco has to be removed by incubation of an antibody with a part of memrane with background band. Protocol for preparation of crude membranes This protocol will help to prepare crude membrane fractions from very small amount of plant tissue. (1) Homogenize roots or root tips with the buffer without SDS.(2) Centrifuge the homogenate in Eppendorf tubes at 2 000 rpm for 2 min to remove cell wall, nuclei, plastids and mitochondria. (3) Centrifuge the obtained supernatant at 30 000 g for 20 min to separate soluble components. Please use adequate tubes for ultracentrifugation. (4) Suspend the obtained precipitate (crude membranes) with SDS buffer with DTT or beta-mercaptoethanol. (5) Heat the sample solution obtained the step (4) at 70 C for 5 min. (6) Apply the denatured sample to SDS-PAGE.Courtesy Prof. Msayoshi Maeshima, Nagoya University, Japan
Application Details:
1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
antibodies to plasma membrane proteinsPlant protein extraction buffer Secondary antibodies
Molecular Weight:
24.5 | 36 kDa
Not reactive in:
Nicotiana benthamiana
Selected references:
Nagasaki et al. (2008) A hydrophilic cation-binding protein of Arabidopsis thaliana, AtPCaP1, is localized to plasma membrane via N-myristoylation and interacts with calmodulin and the posphatidylinositol phosphates PtdIns(3,4,5)P3 and PtdIns(3,5)P2. FEBS J. 275: 2267-2282.Ide et al. (2007) Molecular properties of a novel, hydrophilic cation-binding protein associated with the plasma membrane. J. Exp. Botany, 58: 1173-1183.
Special application note:
Serum contains less than 0.1 % of sodium azide as preservative.
Histone H3-like centromeric protein HTR12 replaces formal H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. It is required for the assembly of kinetochore proteins, mitotic progression and chromosome segregation. Localized at centromeres in both m itotic and meiotic cells. Synonymes: CENH3
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Store reconstituted antibodies at 4°C. Shelf life is 6 months from a date of receipt.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Crucihimalaya himalaica, Turritis glabra Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from N-terminal domain of Arabidopsis thaliana HTR12 AT1G01370 (Q8RVQ9)
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane. F-type ATPases have two components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.Alternative names: ATPase subunit II, ATP synthase F(0) sector subunit b'
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Chlamydomonas reinhardtii
Expected Species:
Algae, Oryza sativa, Sorghum bicolor, Zea mays, Volvox carteri Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated mix of synthetic peptide derived from Arabidopsis thaliana AtpG Q0WMW8, At4g32260 and Chlamydomonas reinhardtii ATP synthase subunit b' A8J785
The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Good signal is obtained with this antibody with a load from 0.5 chlorophyll µg/well.
Application Details:
1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
AS14 2824 | Anti-PsbO1 | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodies AS14 2825 | Anti-PsbO2 | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodiesAS06 142-33 | Anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodiesAS06 167 | Anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodiesAS08 305 | Anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodiesAS06 142-16 | Anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodiesPlant protein extraction buffer | AgriseraSuperDeal
Toubiana et al. (2020). Correlation-based Network Analysis Combined With Machine Learning Techniques Highlight the Role of the GABA Shunt in Brachypodium Sylvaticum Freezing Tolerance. Sci Rep , 10 (1), 4489Wang et al. (2019). YR36/WKS1-mediated Phosphorylation of PsbO, an Extrinsic Member of Photosystem II, Inhibits Photosynthesis and Confers Stripe Rust Resistance in Wheat. Mol Plant. 2019 Oct 14. pii: S1674-2052(19)30330-2. doi: 10.1016/j.molp.2019.10.005.An et al. (2019). Protein cross-interactions for efficient photosynthesis in the cassava cultivar SC205 relative to its wild species. J Agric Food Chem. 2019 Jul 19. doi: 10.1021/acs.jafc.9b00046.Rozpądek et al. (2018). Acclimation of the photosynthetic apparatus and alterations in sugar metabolism in response to inoculation with endophytic fungi. Plant Cell Environ. 2018 Dec 5. doi: 10.1111/pce.13485.Li et al. (2018). Comparative proteomic analysis of key proteins during abscisic acid-hydrogen peroxide-induced adventitious rooting in cucumber (Cucumis sativus L.) under drought stress. Journal of Plant Physiology Volume 229, October 2018, Pages 185-194.Mazur et al. (2016). Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. BMC Plant Biol. 2016 Sep 2;16(1):191. doi: 10.1186/s12870-016-0883-4.Albanese et al.(2016). Isolation of novel PSII-LHCII megacomplexes from pea plants characterized by a combination of proteomics and electron microscopy. Photosynth Res. 2016 Jan 9.Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658. Hu et al. (2015). Site-specific Nitrosoproteomic Identification of Endogenously S-Nitrosylated Proteins in Arabidopsis. Plant Physiol. 2015 Feb 19. pii: pp.00026.2015.Casanova-Sáez et al. (2014). Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development. J Exp Bot. 2014 Mar 24.Albus et al. (2012). LCAA, a novel factor required for Mg protoporphyrin monomethylester cyclase accumulation and feedback-control of aminolevulinic acid biosynthesis in tobacco. Plant Physiol. Oct 19.
Special application note:
Loading based on 50-100 ng of chlorophyll is enough to obtain good signal with this antibody
V-ATPase subunit a is coded by VHA-A2 gene. It has hydrogen ion transmembrane transporter activity. Alternative names: At2g21410/F3K23.17, putative vacuolar proton-ATPase subunit, V-ATPase subunit a (100 kDa subunit), VHA-a
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Chlamydomonas reainhardtii, Physcomitrella patens, Populus balsamifera, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-cougted synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit a, Q9SJT7, At2g21410
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Application Details:
1 : 8000 (ELISA), 1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to vacuolar proteins AS09 467 | Anti-V-ATPase subunit A | vacuolar H+-ATPase, rabbit antibodiesAS09 503 | Anti-V-ATPase, B | vacuolar ATP synthase subunit beta, rabbit antibodiesAS09 468 | Anti-V-ATPase subunit c | vacuolar H+-ATPase, subunit c (16 kDa), rabbit antibodiesAS09 497 | Anti- V-ATPase subunit D |V-type proton ATPase subunit D, rabbit antibodiesAS07 213 | Anti-V-ATPase subunit E of tonoplast H+ATPase, rabbit antibodiesAS09 499 | Anti-V-ATPase subunit H |V-type proton ATPase subunit H, rabbit antibodiesPlant protein extraction bufferSecondary antibodies
Molecular Weight:
93 | 100 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Xing et al. (2016). Proteome Profile of Starch Granules Purified from Rice (Oryza sativa) Endosperm. PLoS One. 2016 Dec 19;11(12):e0168467. doi: 10.1371/journal.pone.0168467.Migocka et al. (2013). NO3 (-)/H(+) Antiport in the Tonoplast of Cucumber Root Cells Is Stimulated by Nitrate Supply: Evidence for a Reversible Nitrate-Induced Phosphorylation of Vacuolar NO3 (-)/H(+) Antiport.PLoS One. 2013 Sep 11;8(9):e73972. doi: 10.1371/journal.pone.0073972.Fumiyoshi et al. (2005). Novel type aquaporin SIPs, are mainly localized the ER membrane and show cell-specific expression in Arabidopsis thaliana. FEBS Lett. 579: 5814-58200.Yoshihiro et al. (2004). Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. Plant Cell Physiol. 45: 1749-1758.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar membranes are used, one µg load per well should be sufficient.Protocol of isolation of plant vacuolar membranes can be found here.
V-ATPase subunit A is a catalytic subunit of V1 complex of vacuolar ATPase. This enzyme (EC=3.6.3.14) is involved in acidification process of various compartements of eucaryotic cell. This protein is coded by VHA-A gene. Alternative names: Vacuolar proton pump subunit alpha, vacuolar H(+)-ATPase subunit A, V-ATPase 69 kDa subunit
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Application Details:
1 : 8000 (ELISA), 1 : 2000 (WB)
Purity:
Ammonium sulfate purified IgG
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
Collection of antibodies to other vacuolar membrane proteinsPlant protein extraction bufferSecondary antibodies
Molecular Weight:
68.8 | 70 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Vera-Estrella et al. (2017). Cadmium and zinc activate adaptive mechanisms in Nicotiana tabacum similar to those observed in metal tolerant plants. Planta. 2017 Apr 28. doi: 10.1007/s00425-017-2700-1.Barkla et al. (2016). Single-cell-type quantitative proteomic and ionomic analysis of epidermal bladder cells from the halophyte model plant Mesembryanthemum crystallinum to identify salt-responsive proteins. BMC Plant Biol. 2016 May 10;16(1):110. doi: 10.1186/s12870-016-0797-1.Yoshihiro et al. (2006) Immunochemical analysis of aquaporin isoforms in Arabidopsis suspension-cultured cells. Cells. Biosci.Biotechnol. Biochem. 70: 980-987.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube label.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for isolation of plant vacuolar membranes can be found here.
Plasma membrane aquaporin, PIP2;7 is water channel protein required for water transport across cell membrane. Alternative names: plasma membrane intrinsic protein 2-7, AtPIP2;7, plasma membrane intrinsic protein 3, salt stress-induced major intrinsic protein, PIP3a
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Jatropha curcas L. cv. Biji Jarak
Expected Species:
Brassica oleracea, Juglans regia, Populus tremulax Populus tremuloides, Spinacia oleracea, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PIP2;7,P93004, At4g35100
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Theoretically this antibody can also react with PIP2;8 but it has not been confirmed experimentally.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Pou et al. (2016). Salinity-mediated transcriptional and post-translational regulation of the Arabidopsis aquaporin PIP2;7. Plant Mol Biol. 2016 Sep 26.Hachez et al. (2014). The Arabidopsis Abiotic Stress-Induced TSPO-Related Protein Reduces Cell-Surface Expression of the Aquaporin PIP2;7 through Protein-Protein Interactions and Autophagic Degradation. Plant Cell. 2014 Dec;26(12):4974-90. doi: 10.1105/tpc.114.134080. Epub 2014 Dec 23.Jang et al. (2013). Twoaquaporins of Jatropha are regulated differentially during drought stress and subsequent recovery. J Plant Physiol. March 25.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of plasma membranes are used, one µg load per well should be sufficient.This antibody has a potential to work in immunolocalization studies, as it is recognizing N-terminall part of the sequence, which is exposed to the cytoplasm.
PDR8 protein is a key factor which controls the extent of cell death in defence response. Alternative names: ABC transporter ABCG.36, AtABCG36, pleiotropic drug resistance protein 8, protein PENETRATION 3
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PDR8, Q9XIE2, At1g59870
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Protocol for preparation of plant plasma membranes can be found under "more information" tab. Additoinal 160 kDa cross-reacting band is detected when working with chloroplast fractions.
Application Details:
1 : 8000 (ELISA), 1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to plant plasma membrane proteinsPlant protein extraction bufferSecondary antibodies
Molecular Weight:
165 |150 kDa (Arabidopsis thaliana)
Not reactive in:
Triticum sp.
Selected references:
Campe at al. (2015). ABC transporter PEN3/PDR8/ABCG36 interacts with calmodulin that, like PEN3, is required for Arabidopsis nonhost resistance. New Phytol. 2015 Aug 28. doi: 10.1111/nph.13582.Yoshihiro et al. (2006). Loss of AtPDR8, a plasma membrane ABC transporter of Arabidopsis thaliana, causes hypersensitive cell death upon pathogen infection. Plant Cell Physiol. 47:309-318.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
MRP1 (EC= 3.6.3.44) is a pump for glutathione S-conjugates. Alternative names: ABC transporter ABCC.1, multidrug resistance-associated protein 1, glutathione S-conjugate-transporting ATPase 1, ATP-energized glutathione S-conjugate pump 1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Oryza sativa, Populus balsamifera, Physcomitrella patens, Triticum aestivum, Vitis vinifera Species of your interest not listed? Contact us
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Application Details:
1 : 8000 (ELISA), 1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to tonoplast proteinsPlant protein extraction bufferSecondary antibodies
Molecular Weight:
181.9 |180 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Geisler et al., (2004). Arabidopsis Immunophilin-like TWD1 Functionally Interacts with Vacuolar ABC Transporters.Mol. Biol. Cell. 15, 3393-3405.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
Nitrate transporter functions in transporter activity in response to jasmonic acid stimulus and response to wounding. This protein is coded by ntp2 gene.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Application Details:
1 : 8000 (ELISA), 1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to other proteins localized in tonoplastPlant protein extraction bufferSecondary antibodies
Molecular Weight:
63.5 | 70 kDa (Arabidopsis thaliana)
Not reactive in:
Solanum tuberosum
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.This protein is present in plant tissues in very low levels.
Glutamate receptor 1.3 might function as non-selective cation channel.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Raphanus sativus
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana UniProt: Q9FH75,TAIR: At5g48410
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Application Details:
1 : 8000 (ELISA), 1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to tonoplast proteins Plant protein extraction bufferSecondary antibodies
Molecular Weight:
97 | 100 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.This protein is expressed in low levels in plant tissues.
ADP ribosylation factor 2 is a GTP-binding protein which is involved in protein trafficking. ArfAlternative name: ARF1-like protein U5
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Arabidopsis thaliana
Immunogen:
synthetic peptide derived from Arabidopsis thaliana Q9SRC3
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Application Details:
1 : 8000 (ELISA), 1 : 2000 (WB)
Purity:
Serum
Related products:
collection of antibodies to plant endomembrane transport system Plant protein extraction bufferSecondary antibodies
Molecular Weight:
20.5 | 29 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
Glutathione S-transferase PM24 (EC=2.5.1.18) is an enzyme which performs conjugation of reduced glutathione to a range of exogenous and endogenous hydrophobic compunds. Alternative name: 24 kDa auxin-binding protein, glutathione S-transferse PM24
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufacture by Operon Biotechnologies.
Application Details:
1 : 8000 (ELISA), 1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
antibodies to plant endomembrane proteinsPlant protein extraction bufferSecondary antibodies
Molecular Weight:
24 | 26 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Sun et a. (2019). Comparative Transcriptome Analysis of the Molecular Mechanism of the Hairy Roots of Brassica campestris L. in Response to Cadmium Stress. Int J Mol Sci. 2019 Dec 26;21(1). pii: E180. doi: 10.3390/ijms21010180.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
PTOX (plastid terminal oxidase) is a component of electron transfer chain responsible for desaturation of phytoene, which prevents the generation of reactive oxygen species. It is involved in the differentiation of plastids: chloroplasts, amyloplasts, and etioplasts. PTOX is expressed ubiquitously in plant tissues and is located in the lumen.Synonymes: IM, IM1, immutants, AOX4, alternative oxidase 4, ubiquinol oxidase 4, chloroplastic/chromoplastic
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
In most plants it is a minor polypeptide and consequently enrichment by analyzing membrane fractions for example is recommended.
Application Details:
1 : 4000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
AS10 1625 | Anti-FNR | Ferredoxin-NADP+-oxidoreductase (higher plants), rabbit antibodies Other antibodies to proteins involved in photosynthetic electron transferSecondary antibodies | AgriseraSuperDeal
Molecular Weight:
30 | 37-41 kDa (Arabidopsis thaliana)
Not reactive in:
Phaeodactylum tricornutum
Selected references:
Pralon et al. (2020). Mutation of the Atypical Kinase ABC1K3 Partially Rescues the PROTON GRADIENT REGULATION 6 Phenotype in Arabidopsis thaliana. Front. Plant Sci., 25 March 2020Bolte et al. (2020). Dynamics of the localization of the plastid terminal oxidase PTOX inside the chloroplast. J Exp Bot. 2020 Feb 15. pii: eraa074. doi: 10.1093/jxb/eraa074.Cournac et al. (2000b). Flexibility in photosynthetic electron transport: a newly identified chloroplast oxidase involved in chlororespiration. Philos Trans R Soc Lond B Biol Sci. 2000 Oct 29;355(1402):1447-54Cournac et al. (2000a). Electron flow between photosystem II and oxygen in chloroplasts of photosystem I-deficient algae is mediated by a quinol oxidase involved in chlororespiration. J Biol Chem. 2000 Jun 9;275(23):17256-62.Josse et al. (2000). A Plastid Terminal Oxidase Associated with Carotenoid Desaturation during Chromoplast Differentiation. Plant Physiol. 2000 Aug;123(4):1427-36
Special application note:
This product can be sold containing proclin if requested.
TIP1;1 is a water channel protein needed for the transport of water, diffusion of amino acids and peptides. Alternative name: Tonoplast intrinsic protein 1-1, gamma-tonoplast intrinsic protein, Gamma-TIP, aquaporin TIP, Tonoplast intrinsic protein, root-specific RB7
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Brassica oleracea, Gossypium mexicanum, Oryza sativa, Pisum sativum, Picea abies, Popoulus trichocarpa, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana UniProt: P25818,TAIR:At2g36830
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Application Details:
1 : 8000 (ELISA), 1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to tonoplast proteins AS09 493 | Anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma), rabbit antibodiesAS09 494 | Anti-TIP2;1 (delta) | Delta-VM23, rabbit antibdiesAS09 510 | Anti-TIP2;1 | tonoplast intrinistic protein 2-1, rabbit antibodiesAS09 511 | Anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal), rabbit antibodiesPlant protein extraction bufferSecondary antibodies
Molecular Weight:
25.6 | 24 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Kwon et al. (2018). AtCAP2 is crucial for lytic vacuole biogenesis during germination by positively regulating vacuolar protein trafficking. Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):E1675-E1683. doi: 10.1073/pnas.1717204115.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.TIP1;1 usually shows a faint band of dimeric form at 42 kDa in addition to the major monomoer band of 23 kDa even in the presence of SDS.
CCaP1 acts as a mediator in response to continuous dark or gibberellic acid. Alternative name: At1g62480/T3P18_4, T3P18.4
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Application Details:
1 : 8000 (ELISA), 1 : 1000 (WB)
Purity:
Serum
Related products:
collection of antibodies to tonoplast proteins Plant protein extraction bufferSecondary antibodies
Molecular Weight:
16.6 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Ide et al. (2007). Transcriptional Induction of Two Genes for CCaPs, Novel Cytosolic Proteins, in Arabidopsis thaliana in the Dark. Plant Cell Physiol. 1:54-65.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
Coproporphyrinogen III oxidase is an enzyme in the biosynthesis of tetrapyrroles. This isoform is encoded by a single nuclear gene in Chlamydomonas reinhardtii. The abundance of the protein increases in copper deficient cells. The protein is localized to the chloroplast.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Physcomitrella patens
Expected Species:
Arabidopsis thaliana, Zea mays Species of your interest not listed? Contact us
Immunogen:
residues 32-366 from mature coproporphyrinogen III oxidase, isoform CPX1 of Chlamydomonas reinhardtii fused to TrxA Q9S7V1
AS05 081 | Anti-CPX2 | coproporphyrinogen III oxidase, isoform 2, rabbit antibodiesCollection of antibodies to Chlamydomonas proteinsAlgal protein extraction buffer Secondary antibodies
Molecular Weight:
41.4 | 38 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).Quinn et al. (1999) Induction of Coproporphyrinogen Oxidase in Chlamydomonas Chloroplasts Occurs via Transcriptional Regulation of Cpx1 Mediated by Copper-Response Elements and Increased Translation from a Copper-Deficiency-Specific Form of the Transcript. J. Biol. Chem. 274:14444-14454.
MTP1 is mediating zinc accumulation in roots and resistance to zinc. Is involved in sequestering of zinc from cytoplasm into vacuole in conditions of its excess. Alternative names: Zinc transporter ZAT-1, ZAT1p, 43.8 | metal tolerance protein 1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Nicotiana tabacum
Expected Species:
Brassica sp. , Noccaea fendleri, Thlaspi caerulescens Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana MTP1 UniProt:Q9ZT63, TAIR:At2g46800
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Application Details:
1 : 8000 (ELISA), 1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to plant tonoplast membrane proteinsAS09 474 | Anti-AtMTP4 | metal tolerance protein B, rabbit antibodiesPlant protein extraction bufferSecondary antibodies
Molecular Weight:
43.8 | 43 kDa (Arabidopsis thaliana)
Not reactive in:
Members of Solanaceae
Selected references:
Vera-Estrella et al. (2017). Cadmium and zinc activate adaptive mechanisms in Nicotiana tabacum similar to those observed in metal tolerant plants. Planta. 2017 Apr 28. doi: 10.1007/s00425-017-2700-1.Kawachi et al. (2008). Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn(2+)/H(+) antiporter of Arabidopsis thaliana, stimulates the transport activity. J.Biol. Chem. 13:8374-8383.Kobae et al. (2004). Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis. Plant Cell Physiol. 12:1749-1758.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.MTP1 protein is of low abundance in plant tissues.
Ca2+-ATPase | calmodulin-stimulated calcium-ATPase belongs to family of cation transport ATPase (P-type).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Application Details:
1 : 8000 (ELISA), 1 : 1000 (WB)
Purity:
Serum
Related products:
collection of antibodies to tonoplast proteins Plant protein extraction bufferSecondary antibodies
Molecular Weight:
111 | 110 kDa (Raphanus sativus)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Siddiqui et al. (2020). Melatonin and calcium function synergistically to promote the resilience through ROS metabolism under arsenic-induced stress. Journal of Hazardous Materials Volume 398, 5 November 2020, 122882
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.This protein is of low abundance in plant tissues.
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient. The transmembrane CF0IV subunit of appr. 25 kDa belongs to a stator part of ATP synthase and is involved in the proton translocation.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4°C. Long-term -20°. Repeated freezing and thawing is not recommended. Solution contains 0.01% sodium azide as preservative.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.
The retinoblastoma protein Rb is considered to be a key regulator of G1/S phase transition by blocking S phase entry and cell growth. Plant retinoblastoma-related (RBR) proteins share a homology with the human tumour suppressor retinoblastoma (pRb) protein. RBR protein functions are controlled by phosphorylation and protein-protein interactions. Short name: AtRBR
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in PBS, pH 8.5 with 0.02 % sodium azide
Storage Temp:
Store at 4°C. Upon arrival please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Chicken
Species Reactivity:
Arabidopsis thaliana, Medicago sativa
Expected Species:
Camelia sinensis, Chenopodium rubrum, Cocos nucifera, Hordeum vulgare, Oryza sativa, Pisum sativum, Populus tremula, Scutelaria baicalensis, Zea mays Species of your interest not listed? Contact us
Immunogen:
Recombinant C-terminal fragment consisting of 236 amino acids of Arabidopsis thaliana retinoblastoma protein UniProt: Q9LKZ3, lTAIR: At3g12280
Applications:
Chromatin IP (ChIP), Immunoprecipitation (IP), Western blot (WB)
This antibody is not suitable for immunolocalization.Methanol concentration in a transfer buffer can be considerably reduced or for a better transfer of high MW proteins (even with PVDF membrane).For immunoprecipitation start with 2 µl and titrate it depending upon your experimental conditions. Please note that you work with a total IgY fraction, which means that it will contain between 40-60 µg of total IgY (directed not only against retinoblastoma) therefore all of this IgY needs to be captured by the anti-IgY matrix.As control pre-serum for IP this product can be used, total, pre-immune IgY.
Application Details:
2µl (IP), 1 : 2000 (WB)
Purity:
Total IgY
Related products:
Collection of antibodies for plant developmental biologyPlant protein extraction buffer Secondary antibodies
Molecular Weight:
112 kDa
Not reactive in:
Chlamydomonas reinhardtii
Selected references:
Leviczky et al. (2019). E2FA and E2FB transcription factors coordinate cell proliferation with seed maturation. Development. 2019 Nov 26;146(22). pii: dev179333. doi: 10.1242/dev.179333.Horvath et al. (2017). Arabidopsis RETINOBLASTOMA RELATED directly regulates DNA damage responses through functions beyond cell cycle control. EMBO J. 2017 May 2;36(9):1261-1278. doi: 10.15252/embj.201694561. Epub 2017 Mar 20.Cheng et al. (2013). Down-regulation of multiple CDK inhibitor ICK/KRP genes up-regulates E2F pathway and increases cell proliferation, organ and seed sizes in Arabidopsis. Plant j. May 7.Ábrahám et al. (2011). Immunodetection of retinoblastoma-related protein and its phosphorylated form in interphase and mitotic alfalfa cells. J Exp Bot 62(6):2155-2168.
PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across cell membrane.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Peptide used to elicit this antibody is also conserved in Arabidopsis thaliana PIP2;1,PIP2;2, PIP2;3 and this antibody reacted also with PIP2;2 in experiments with knock out plants.
Markus G. et al., (2004). Arabidopsis Immunophilin-like TWD1 Functionally Interacts with Vacuolar ABC Transporters.Mol. Biol. Cell. 15, 3393-3405.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.
PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across cell membrane. PIP1;2 is also designated as PIP1B.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.For long time storage over 1 year please dilute the antibody solution 10 fold with 10 mM Tris-buffered saline containing 10% glycerol. Glycerol may suppress denaturation of protein.
Lupinus sp. , Hordeum vulgare, Solanu lycopersicum, Ricinus communis, Oryza sativa, Populus trichocarpa Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from N terminus of Raphanus sativus PAQ1O80368. Chosen peptide is conserved in PIP1;1, PIP1;2, PIP1;3 N-terminus of Raphanus sativus and in all 5 isoforms of Arabidopsis thaliana coded by:AT3G61430.1 (PIP1;1), AT2G45960.3 (PIP1;2), AT1G01620.1 (PIP1;3), AT4G00430.1 (PIP1;4), AT4G23400.1 (PIP1;5)
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Antibody reacts also with PIP2 isoforms in Zea mays.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Patankar et al. (2019). Functional Characterization of Date Palm Aquaporin Gene PdPIP1;2 Confers Drought and Salinity Tolerance to Yeast and Arabidopsis. Genes (Basel). 2019 May 22;10(5). pii: E390. doi: 10.3390/genes10050390.Fernández-San Millán et al. (2018). Physiological Performance of Transplastomic Tobacco Plants Overexpressing Aquaporin AQP1 into Chloroplast Membranes. J Exp. Bot. ery148, https://doi.org/10.1093/jxb/ery148.Pengelly et al. (2014). Transplastomic integration of a cyanobacterial bicarbonate transporter into tobacco chloroplasts. J Exp. Botany, doi:10.1093/jxb/eru156.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
Cyclophilin (CYP37) represents the homologous component of the AtCYP38. The first complex immunophilin protein identified from higher plants. The AtCYP38 was initially designated as TLP40 (thylakoid lumen protein). Alternative names: rotamase CYP37, TLP38, p38.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store short-term 4°C. Long term -20°C.. Repeated freezing and thawing is not recommended. Solution contains 0.01% sodium azide.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Ricinus communis Species of your interest not listed? Contact us
Immunogen:
Synthetic peptide (amino acids 277 – 290) specific for chloroplast cyclophilin from Arabidopsis thaliana (At3g15520) (P82869)
HSP90-2 (heat shock protein 90-2) is a constitutive isoform of HSP90, expressed in all tissues and abundant in root apical meristem, pollen and tapetum. Alternative names: ATHSP90.2, EARLY-RESPONSIVE TO DEHYDRATION 8, ERD8, HEAT SHOCK PROTEIN 90.2, HEAT SHOCK PROTEIN 81-2, HEAT SHOCK PROTEIN 81.2, HEAT SHOCK PROTEIN 90.2, HSP81-2, HSP81.2, HSP90.2.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Glycne max, Hordeum vulgare, Micromonas pulsilla, Nicotiana benthamina, Nicotiana tabacum, Ostreococcus lucimarinus, Oryza sativa, Physcomitrella patens, Populus balsamifera, Ricinus communis, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
Full length recombinant HSP90-2 of Arabidopsis thaliana, UniProt: F4K6B6-1, TAIR: AT5G56030
This product can be sold containing ProClin if requested.
Application Details:
1 : 3000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water
Related products:
collection of antibodies to plant HSP proteinsPlant protein extraction bufferSecondary antibodies | AgriseraSuperDeal
Molecular Weight:
80.6 | 95 kDa (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Barghetti et al. (2017). Heat-shock protein 40 is the key farnesylation target in meristem size control, abscisic acid signaling, and drought resistance. Genes Dev. 2017 Nov 15;31(22):2282-2295. doi: 10.1101/gad.301242.117.He et a. (2012).SpecificMissenseAlleles of theArabidopsisJasmonicAcidCo-ReceptorCOI1RegulateInnateImmuneReceptorAccumulation andFunction. PloS Genetics, Open Access.
Special application note:
Antibody reacts more with constitutive isofrom Hsp90-2 than heat inducible Hsp90-1.
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Antiboy will weakly react with PIP2;1 and theoretically with PIP2;3, however this protein is very scarcely expressed in plant tissues. The mRNA content is 1 % of that for PIP2;2, therefore reactivity to PIP2;3 was neglegible in our experiments.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.
Application Details:
1 : 8000 (ELISA), 1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to plasma membrane proteins AS09 488 | Anti-PIP2;1 | aquaporin PIP2;1, rabbit antibodiesAS09 489 | Anti-PIP1;1, PIP1;2, PIP1;3 | aquaporins, rabbit antibodiesAS09 491 | Anti-PIP2;1,PIP2;2,PIP2;3 |plasma membrane intrinistic protein 2-1,2-2, 2-3, rabbit antibodiesPlant protein extraction bufferSecondary antibodies
Molecular Weight:
30 | 28 kDa (Raphanus sativus)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
PIP2;2 is a plasma membrane aquaporin. Alternative names of isoforms: aquaporin PIP2-1, plasma membrane intrinsic protein 2a, PIP2a, aquaporin PIP2-2, plasma membrane intrinsic protein 2b, PIP2b, TMP2b, Aquaporin PIP2-3, plasma membrane intrinsic protein 2c, PIP2c, TMP2C, RD28-PIP, water stress-induced tonoplast intrinsic protein, (WSII-TIP)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Brassica napus, Cucumis sativus, Glycine hispida, Gossypium hirsutum, Hedychium coronarium, Mimosa saman, Nicotiana glauca, Petunia hybrida, Pisum sativum, Ricinus communis, Populus tremula x Populus tremloides, Physcomitrella patens Species of your interest not listed? Contact us
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Hyun-Sung et al. (2019). NaCl-induced CsRCI2E and CsRCI2F interact with aquaporin CsPIP2;1 to reduce water transport in Camelina sativa L. Biochemical and Biophysical Research Communications,Available online 4 April 2019.Chowanski et al. (2015). Cold induced changes in lipid, protein and carbohydrate levels in the tropical insect Gromphadorhina coquereliana. Comp Biochem Physiol A Mol Integr Physiol. 2015 May;183:57-63. doi: 10.1016/j.cbpa.2015.01.007. Epub 2015 Jan 23.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at at Store short-term 4°C. Long-term -20°. Repeated freezing and thawing is not recommended.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Blair et al. (2018). The Helicobacter pylori cell shape promoting protein Csd5 interacts with the cell wall, MurF, and the bacterial cytoskeleton. Mol Microbiol. 2018 Jul 24. doi: 10.1111/mmi.14087.Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.
TIPs belong to MIP/aquaporin protein family. Alternative names: gamma-tonoplast intrinsic protein, gamma-TIP, aquaporin TIP, tonoplast intrinsic protein, root-specific RB7, gamma-tonoplast intrinsic protein 2, gamma-TIP2, salt stress-induced tonoplast intrinsic protein
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Raphanus sativus
Expected Species:
Brassica napus Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide conserved in Raphanus sativus TIP1;1 and TIP1;2 (protein accesion number available in Suga et al. (2001).
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Suga et al. (2001). Specificity of the accumulation of mRNAs and proteins of the plasma membrane and tonoplast aquaporins in radish organs. Planta 212:294-304.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
Ribosomal protein S1, chloroplastic, is a protein involved in the initiation complex formation via a strong mRNA-binding activity. It has a poly(A)-binding activity, this might play a significant role as a control element in chloroplast mRNA translation.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
For reconstitution add 100 µl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Capsella rubella, Citrus sinensis, Cucumis sativus, Brassica napus, Gossypium arboreum, Populus trichocarpa, Ricinus communis, Solanum tuberosum, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
Recombinant S1 protein from spinach Spinacia oleracea, UniProt: P29344
AS13 2650 | Anti-L13-1 | 60S ribosomal protein L13-1, rabbit antibodies AS09 478| Anti-L13-1 | 60S ribosomal protein L13-1, rabbit antibodies AS08 331| Anti-L-30 | 50S ribosomal protein L30, rabbit antibodiesAS11 1738| Anti-Rpl1 | 50S ribosomal protein L1, rabbit antibodies AS15 2876 | Anti-RPL2 | ribosomal protein L2 (chloroplastic), rabbit antibodies AS14 2780 | Anti-RPL33 | 50S ribosomal protein L33, chloroplastic, rabbit antibodies AS14 2781 | Anti-RPL36 | 50S ribosomal protein L36, chloroplastic, rabbit antibodiesAS12 2115 | Anti-RPL37 | ribosomal protein L37, cytoplasmic, rabbit antibodiesAS15 2875 | Anti-RPS1 | ribosomal protein S1 (chloroplastic), rabbit antibodiesAS12 2114 | Anti-RPS12 | ribosomal protein S12, (chloroplastic), rabbit antibodiesAS14 2779 | Anti-RPS15 | 30S ribosomal protein S15, (chloroplastic), rabbit antibodiesAS10 719 | Anti-Rps2 | anti-ribosomal subunit 2 (cytoplasmic), rabbit antibodiesAS16 3832 | Anti-RPS2 | Ribosomal protein S2 (mitochondrial), rabbit antibodiesAS08 309 | Anti-S1 | anti-30S ribosomal protein S1, rabbit antibodiesAS12 2111 | Anti-S14 | 40S ribosomal protein S14-1 , rabbit antibodiesAS15 3068 | Anti-S4 | mitochondrial ribosomal small subunit protein S4, rabbit antibodiesAS15 3067 | Anti-S10 | mitochondrial ribosomal small subunit protein S10, rabbit antibodiesAS15 3069 | Anti-L16 mitochondrial ribosomal large subunit protein L16, rabbit antibodiesSecondary antibodies
Molecular Weight:
45.1 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Wang et al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 .Swift et al. (2020). Functional Analysis of PSRP1, the Chloroplast Homolog of a Cyanobacterial Ribosome Hibernation Factor. Plants (Basel). 2020 Feb 6;9(2). pii: E209. doi: 10.3390/plants9020209.Swift et al. (2020). Functional Analysis of PSRP1, the Chloroplast Homolog of a Cyanobacterial Ribosome Hibernation Factor. Plants (Basel). 2020 Feb 6;9(2). pii: E209. doi: 10.3390/plants9020209.
TIPs belong to MIP/aquaporin protein family. Alternative names: Aquaporin TIP1-1,gamma-tonoplast intrinsic protein, gamma-TIP, aquaporin TIP, gamma-tonoplast intrinsic protein, gamma-TIP, aquaporin TIP, tonoplast intrinsic protein, root-specific RB7, gamma-tonoplast intrinsic protein 2, gamma-TIP2, salt stress-induced tonoplast intrinsic protein
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Raphanus sativus
Expected Species:
Brassica napus, Gossypium hirsutum, Hordeum vulgare, Oryza sativa, Populus trichocarpa, Ricinus communisSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide conserved in Raphanus sativus TIP1;1 and TIP1;2 (protein accesion number available in Suga et al. 2001). Peptide is also conserved in Arabidopsis thaliana TIP1-1 P25818, At2g36830, TIP1-2 Q41963, At3g26520
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.
Application Details:
1 : 8000 (ELISA), 1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to tonoplast proteins AS09 492 | Anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma), rabbit anitbodiesAS09 494 | Anti-TIP2;1 (delta) | Delta-VM23, rabbit anitbodiesAS09 510 | Anti-TIP2;1 | tonoplast intrinistic protein 2-1, rabbit anitbodiesAS09 511 | Anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal), rabbit anitbodiesAS09 493P | TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma) | blocking peptidePlant protein extraction bufferSecondary antibodies
Molecular Weight:
25.8 | 23 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Mao and Sun (2015). Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3. J Exp Bot. 2015 May 26. pii: erv244.Suga et al. (2001). Specificity of the accumulation of mRNAs and proteins of the plasma membrane and tonoplast aquaporings in radish organs. Planta 212:294-304.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
Ribosomal protein L2, chloroplastic, is one of the primary proteins involved in rRNA-binding and is a constituent of the large subunit of the ribosomal complex.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
For reconstitution add 100 µl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Brassica napus, Cannabis sativa, Capsella rubella, Citrus sinensis, Cucumis sativus, Gossypium arboreum, Populus trichocarpa, Solanum lycopersicum, Solanum tuberosum, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
Full length, recombinant RPL2 protein from Arabidopsis thaliana, Uniprot: P56791, TAIR:ATCG00830
The RPL2 protein is a small protein that should be best analysed using 14% SDS PAGE as described by Schägger (2006, Nature Protocols, 1, 16-20).Following sucrose gradient centrifugation of maize chloroplasts this antibody detects two bands that are very similar in size.
Application Details:
1 : 3000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to proteins involved in photosynthesisAS13 2650 | Anti-L13-1 | 60S ribosomal protein L13-1, rabbit antibodies AS09 478| Anti-L13-1 | 60S ribosomal protein L13-1, rabbit antibodies AS08 331| Anti-L-30 | 50S ribosomal protein L30, rabbit antibodiesAS11 1738| Anti-Rpl1 | 50S ribosomal protein L1, rabbit antibodies AS14 2780 | Anti-RPL33 | 50S ribosomal protein L33, chloroplastic, rabbit antibodies AS14 2781 | Anti-RPL36 | 50S ribosomal protein L36, chloroplastic, rabbit antibodiesAS12 2115 | Anti-RPL37 | ribosomal protein L37, cytoplasmic, rabbit antibodiesAS15 2875 | Anti-RPS1 | ribosomal protein S1 (chloroplastic), rabbit antibodiesAS12 2114 | Anti-RPS12 | ribosomal protein S12, chloroplastic, rabbit antibodiesAS14 2779 | Anti-RPS15 | 30S ribosomal protein S15, chloroplastic, rabbit antibodiesAS10 719 | Anti-Rps2 | anti-ribosomal subunit 2 (cytoplasmic), rabbit antibodiesAS08 309 | Anti-S1 | anti-30S ribosomal protein S1, rabbit antibodiesAS12 2111 | Anti-S14 | 40S ribosomal protein S14-1 , rabbit antibodiesAS09 477 | Anti-S14 | 40S ribosomal protein S14-1, rabbit antibodiesAS15 3068 | Anti-S4 | mitochondrial ribosomal small subunit protein S4, rabbit antibodiesAS15 3067 | Anti-S10 | mitochondrial ribosomal small subunit protein S10, rabbit antibodiesAS15 3069 | Anti-L16 mitochondrial ribosomal large subunit protein L16, rabbit antibodiesSecondary antibodies
Molecular Weight:
29.9 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Wang et al. (2020) Rerouting of ribosomal proteins into splicing in plant organelles. BioRxiv, DOI: 10.1101/2020.03.03.974766 .Jeon et al. (2017). Functional characterization of chloroplast-targeted RbgA GTPase in higher plants. Plant Mol Biol. 2017 Nov;95(4-5):463-479. doi: 10.1007/s11103-017-0664-y.
Ribosomal protein S7, chloroplastic, is a protein that is a part of the small subunit of the ribosomal complex. It is one of the primary rRNA binding proteins.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
For reconstitution add 100 µl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Brassica napus, Cannabis sativa, Capsella rubella, Citrus sinensis, Cucumis sativus, Gossypium arboreum, Ricinus communis, Populus trichocarpa, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
full length, recombinant S7 protein from Arabidopsis thaliana, UniProt: P61841, TAIR: ATCG00900
TIPs belong to MIP/aquaporin protein family. Alternative name of radish TIP2;1 (delata) is delta-VM23.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Raphanus sativus
Expected Species:
Raphanus sativus
Immunogen:
KLH-synthetic peptide conserved in Raphanus sativus TIP2;1 (delta) O82142
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.
Application Details:
1 : 8000 (ELISA), 1 : 1000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to tonoplast proteins AS09 492 | Anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma), rabbit antibodiesAS09 493 | Anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma), rabbit antibodiesAS09 510 | Anti-TIP2;1 | tonoplast intrinistic protein 2-1, rabbit antibodiesAS09 511 | Anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal), rabbit antibodiesPlant protein extraction buffer
Molecular Weight:
24.9 | 23 kDa
Not reactive in:
Arabidopsis thaliana, Pisum sativum
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.42 kDa dimer is also detected by those antibodies.
Ketol-acid reductoisomerase is a catalytic enzyme in the biosynthetic pathway of amino acid biosynthesis. This protein catalyzes the reductive isomerization of 2-acetolactate to 2,3-dihy-droxyisovalerate.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
For reconstitution add 100 µl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Brassica napus, Capsella rubella, Citrus sinensis, Cucumis sativus, Gossypium arboreum, Populus trichocarpa, Solanum lycopersicum, Solanum tuberosum, Ricinus communis, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
Full length, recombinant protein KARI protein from Spinacia oleracea, UniProt: Q01292, TAIR:AT3G58610
SIP protein functions as a water channel to facilitate the transport of water across cell membrane. It is mainly localized to ER. Alternative name: small basic intrinistic protein 1-1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Application Details:
1 : 8000 (ELISA), 1 : 1000 (WB)
Purity:
Serum
Related products:
AS09 496 | Anti-AtSIP1;1, rabbit antibodiescollection of antibodies to endomembrane system Plant protein extraction buffer
Molecular Weight:
25.7 | 25 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Ishikawa et al. (2005). Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana. FEBS Lett. 579:5814-5820.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Anti-SIP1;1 antibodies will not recognize SIP2;1 protein.
ACCase subunit beta (acetyl-coenzyme A, carboxylase (subunit beta)) is a component of the acetyl coenzyme A carboxylase (ACC) complex. It is catalyzing carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
For reconstitution add 100 µl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Aethionema grandiflorum, Barbarea verna, Brassica napus, Capsella bursa pastoris, Carica papaya, Cardamine impatiens, Crucihimalaya wallichii, Draba nemorosa, Lepidium virginicum, Lobularia maritima, Nasturtium officinale, Olimarabidopsis pumila, Pachycladon enysii, Raphanus sativus, Theobroma cacao Species of your interest not listed? Contact us
Immunogen:
Recombinant ACC of Arabidopsis thaliana UniProt: P56765, TAIR:ATCG00500
SIP protein functions as a water channel to facilitate the transport of water across cell membrane. It is mainly localized to ER. Alternative name: small basic intrinistic protein 2-1
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Application Details:
1 : 8000 (ELISA), 1 : 1000 (WB)
Purity:
Serum
Related products:
AS09 495 | Anti-AtSIP1;1 | aquaporin SIP1-1, rabbit antibodiescollection of antibodies to endomembrane system Plant protein extraction buffer
Molecular Weight:
25.9 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Barghetti et al. (2017). Heat-shock protein 40 is the key farnesylation target in meristem size control, abscisic acid signaling, and drought resistance. Genes Dev. 2017 Nov 15;31(22):2282-2295. doi: 10.1101/gad.301242.117.Ishikawa et al. (2005). Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana. FEBS Lett. 579:5814-5820.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
Biotin carboxyl carrier subunit of chloroplast ACCase is a biotin carboxyl-carrier subunit of the multi-enzyme plastidial acetyl-coenzyme A carboxylase complex involved in fatty acid biosynthesis process.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
For reconstitution add 100 µl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
V-ATPase is an enzyme responsible for acidification of intracellular compartments. Subunit D is the peripheral subunit of V1 complex. Alternative name: Vacuolar proton pump subunit D, vacuolar H(+)-ATPase subunit D
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Sorghum bicolor, Zea mays Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana V-ATPase subunit D UniProt: Q9XGM1, TAIR: At3g58730
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Application Details:
1 : 8000 (ELISA), 1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to vacuolar proteins AS09 466 | Anti-V-ATPase subunit a | vacuolar H+ ATPase, subunit a, rabbit antibodiesAS09 467 | Anti-V-ATPase subunit A | vacuolar H+-ATPase, rabbit antibodiesAS09 503 | Anti-V-ATPase, B | vacuolar ATP synthase subunit beta, rabbit antibodiesAS09 468 | Anti-V-ATPase subunit c | vacuolar H+-ATPase, subunit c (16 kDa) , rabbit antibodiesASS09 497 | Anti- V-ATPase subunit D |V-type proton ATPase subunit D, rabbit antibodiesAS07 213 | Anti-V-ATPase subunit E of tonoplast H+ATPase, rabbit antibodiesAS09 499 | Anti-V-ATPase subunit H |V-type proton ATPase subunit H, rabbit antibodiesPlant protein extraction buffer
Molecular Weight:
29 | 29 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
The chloroplast ATP synthase belongs to the family of F1-type ATPases, which are also present in bacteria and mitochondria. ATP synthase generates ATP from ADP and inorganic phosphate using energy derived from a trans-thylakoidal electrochemical proton gradient.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at short-term 4°C. Long-term -20°. Repeated freezing and thawing is not recommended. Solution contains 0.01% sodium azide as preservative.
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Galvis et al. (2020). H+ transport by K+ EXCHANGE ANTIPORTER3 promotes photosynthesis and growth in chloroplast ATP synthase mutants. Plant Physiol. pp.01561.2019. doi: 10.1104/pp.19.01561.Koochak et al. (2019). The structural and functional domains of plant thylakoid membranes. Plant J. 2019 Feb;97(3):412-429. doi: 10.1111/tpj.14127.Lv et al. (2019). Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. Plant Cell. 2019 Jan;31(1):210-230. doi: 10.1105/tpc.18.00813.Gao et al. (2018). A supercomplex, approximately 720 kDa and composed of both photosystem reaction centers, dissipates excess energy by PSI in green macroalgae under salt stress. Plant Cell Physiol. 2018 Oct 8. doi: 10.1093/pcp/pcy201.Koochak et al. (2018). The structural and functional domains of plant thylakoid membranes. Plant J. 2018 Oct 12. doi: 10.1111/tpj.14127. (BN-PAGE)Rantala and Tikkanen et al. (2018). Phosphorylation‐induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2.Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658. Grieco et al. (2015). Light-harvesting II antenna trimers connect energetically the entire photosynthetic machinery - including both photosystems II and I. Biochim Biophys Acta. 2015 Jun-Jul;1847(6-7):607-19. doi: 10.1016/j.bbabio.2015.03.004. Epub 2015 Apr 3.Yap at al. (2015). AEF1/MPR25 is implicated in RNA editing of plastid atpF and mitochondrial nad5 and also promotes atpF splicing in Arabidopsis and rice. Plant J. 2015 Jan 13. doi: 10.1111/tpj.12756.
Special application note:
This product can be sold containing proClin if requested.
V-ATPase is an enzyme responsible for acidification of intracellular compartments. Subunit H is part of the peripheral V1 complex. Alternative name: vacuolar proton pump subunit H, vacuolar H(+)-ATPase subunit H
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Expected Species:
Chlamydomonas reinhardtii, Hordeum vulgare, Oryza sativa, Picea sitcHensis, Populus balsamifera subsp. trichocarpa, Spinacia oleracea Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana V-ATPase subunit H UniProt: Q9LX65, TAIR: At3g42050
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Application Details:
1 : 8000 (ELISA), 1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to vacuolar proteins AS09 466 | Anti-V-ATPase subunit a | vacuolar H+ ATPase, subunit a, rabbit antibodiesAS09 467 | Anti-V-ATPase subunit A | vacuolar H+-ATPase, rabbit antibodiesAS09 503 | Anti-V-ATPase, B | vacuolar ATP synthase subunit beta, rabbit antibodiesAS09 468 | Anti-V-ATPase subunit c | vacuolar H+-ATPase, subunit c (16 kDa) , rabbit antibodiesASS09 497 | Anti- V-ATPase subunit D |V-type proton ATPase subunit D, rabbit antibodiesAS07 213 | Anti-V-ATPase subunit E of tonoplast H+ATPase, rabbit antibodiesPlant protein extraction buffer
Molecular Weight:
50 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
Isocitrate lyase (EC=4.1.3.1), localized in glyoxysome is an enzyme involved in mobilization of storage lipis during plant seedling growth. Catalalyzed reaction is a conversion of isocitrate to succinate and glyoxylate. Alternative names of this enzyme: isocitrase, isocitritase, isocitratase, glyoxylate-lyase, isocitrate glyoxylatelyase.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Ricinus communis
Expected Species:
Arabidopsis thaliana, Cucumis sativus, Gossypium mexicanum, Oryza sativa, Populus balsamifera subsp. trichocarpa, Sorghum bicolor Species of your interest not listed? Contact us
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.ICL protein is most abundant in young cotyledons in germinating seedlings, not in rosette leaves or shoots.
Application Details:
1 : 8000 (ELISA), 1 : 2000 (WB)
Purity:
Ammonium sulfate purified IgG
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to proteins involved in carbohydrate metabolism Plant protein extraction bufferSecondary antibodies
Molecular Weight:
64.7 | 65 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Réthoré et al. (2019). Arabidopsis seedlings display a remarkable resilience under severe mineral starvation using their metabolic plasticity to remain self-sufficient for weeks. Plant J. 2019 Mar 22. doi: 10.1111/tpj.14325.Maeshima et al. (1988). Evidence for no proteolytic processing during transport of isocitrate lyase into glyoxysomes in castor bean endosperm. Plant Cell Physiol. 29:381-384.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.
V-ATPase subunit A is a catalytic subunit of V1 complex of vacuolar ATPase. This enzyme (EC=3.6.3.14) is involved in acidification process of various compartements of eucaryotic cell. This protein is coded by VHA-A gene. Alternative names: Vacuolar proton pump subunit alpha, vacuolar H(+)-ATPase subunit A, V-ATPase 69 kDa subunit
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Arabidopsis thaliana, Cucumis sativus, Gossypium mexicanum, Hordeum vulgare, Populus trichocarpa, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
Purified native V-ATPase subunit A from Ricinus communis, UniProt: B9RHV0
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.
Application Details:
1 : 8000 (ELISA), 1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to vacuolar proteins AS09 467 | Anti-V-ATPase subunit A | vacuolar H+-ATPase, rabbit antibodiesAS09 503 | Anti-V-ATPase, B | vacuolar ATP synthase subunit beta, rabbit antibodiesAS09 468 | Anti-V-ATPase subunit c | vacuolar H+-ATPase, subunit c (16 kDa), rabbit antibodiesASS09 497 | Anti- V-ATPase subunit D |V-type proton ATPase subunit D, rabbit antibodiesAS07 213 | Anti-V-ATPase subunit E of tonoplast H+ATPase, rabbit antibodiesAS09 499 | Anti-V-ATPase subunit H |V-type proton ATPase subunit H, rabbit antibodiesPlant protein extraction buffer
Molecular Weight:
63 | 68 kDa (Ricinus communis)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Kawamura et al. (2000). Tissue specificity of E subunit isoforms of plant vacuolar H(+)-ATPase and existence of isotype enzymes. J.Biol. Chem. 275:6515-6522.Nakanishi & Maeshima (1998). Molecular cloning of vacuolar H(+)-pyrophosphatase and its developmental expression in growing hypocotyl of mung bean. Plant Physiol. 116:589-597.Smart et al. (1998). Genes involved in osmoregulation during turgor-driven cell expansion of developing cotton fibers are differentially regulated. Plant Physiol. 116:1539-1549.Matsuura-Eno et al. (1992). Mechanism of the Decline in Vacuolar H -ATPase Activity in Mung Bean Hypocotyls during Chilling. Plant Physiology 100:718-722.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
Chitinase 3 is a protein that hydrolyzes chitin and has a great impact in the defense against fungal pathogens that contains chitin. Alternative names: Basic endochitinase 1, Class I chitinase c, OsChia1c, Pathogenesis related (PR)-3, chitinase 3.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
The antibody may be stored at -20°C for one year in its original formulation. Additionally, antibody may be stored at 2°C to 8°C for up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody.Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Oryza sativa
Expected Species:
Musa paradisiaca, Zea mays Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Oryza sativa OsCht3 protein sequence, UniProt:P24626 CHI3_ORYSJ
AS10 687 | Anti-PR-1 | Pathogenesis-related protein 1, rabbit antibodies AS07 208 | Anti-PR-2 | GLU I | class I beta-1,3-glucanase, rabbit antibodies for other species, not Arabidopsis thaliana AS12 2366 | Anti-PR-2 | pathogenesis-related protein 2, rabbit antibody for Arabidopsis thaliana PR-2 protein detection AS07 207 | Anti-PR-3 | CHN | class I chitinase, rabbit antibodiesAS12 2369 | Anti-PR-4 | Pathogenesis-related protein 4, rabbit antibodiesAS12 2373 | Anti-PR-5 | Pathogenesis-related protein 5, rabbit antibodiescollection of antibodies to other proteins involved in a response to pathogen attackSecondary antibodies
Molecular Weight:
33.68 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Chitinase 8 is involved in the hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins. Alternative names: Chitinase 8, Class II chitinase a, OsChia2a, Pathogenesis related (PR)-3 chitinase 8.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
The antibody may be stored at -20°C for one year in its original formulation. Additionally, antibody may be stored at 2°C to 8°C for up to 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles of the diluted antibody.Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Oryza sativa
Expected Species:
Hordeum vulgare, Triticum aestivum Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide derived from Oryza sativa OsCht3 protein sequence, UniProt: Q7XCK6, CHI8_ORYSJ
AS10 687 | Anti-PR-1 | Pathogenesis-related protein 1, rabbit antibodies AS07 208 | Anti-PR-2 | GLU I | class I beta-1,3-glucanase, rabbit antibodies for other species, not Arabidopsis thaliana AS12 2366 | Anti-PR-2 | pathogenesis-related protein 2, rabbit antibody for Arabidopsis thaliana PR-2 protein detection AS07 207 |Anti-PR-3 | CHN | class I chitinase, rabbit antibodiesAS12 2369 | Anti-PR-4 | Pathogenesis-related protein 4, rabbit antibodiesAS12 2373 | Anti-PR-5 | Pathogenesis-related protein 5, rabbit antibodiesAS15 2888 | Anti-CHIT3 | Chitinase 3, rabbit antibodiescollection of antibodies to other proteins involved in a response to pathogen attackSecondary antibodies
Molecular Weight:
27.55 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
The PsbH protein was originally named 10- or 9-kDa phosphoprotein in higher plant chloroplasts. It is encoded by the plastome in algae and higher plants. PsbH is also present in cyanobacteria, where it exhibits 56% amino acid identity with the corresponding protein from Arabidopsis. The protein contains 63–90 amino acids, depending on the species, with molecular masses between 7.0 and 9.9 kDa.PsbH is an intrinsic membrane protein with a single transmembrane helix and its N-terminal region has been suggested to be exposed to the stromal side of the thylakoid membrane. Presence of PsbH already present in etiolated tissue can indicate that the protein may be involved in early stages of PSII assembly.Obtained biochemical data from PSII complexes isolated from spinach suggest that PsbH, together with other PSII phosphoproteins, may be required for D1 protein turnover by regulating dimeric and monomeric PSII transition through their phosphorylation and dephosphorylation.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Cannabis sativa, Glycine max, Nicotiana tabacum, Oryza sativa, Picea sitchensis, Pisum sativum, Populus deltoides, Solanum lycopersicumSpecies of your interest not listed? Contact us
Immunogen:
KLH-conjugated synthetic peptide chosen from PsbH protein of Arabidopsis thaliana P56780, AtCg00710
LMW proteins can sometimes interfere with chlorophyll, but most chlorophyll can be removed by precipitating sample in acetone before loading on a gel.Protocol: Add acetone to final concentration of 80% ice-cold acetone. Leave 10 minutes. Spin. Rresuspend pellet in solubilisation buffer and load on a gel.
Application Details:
1 : 5000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to PSII proteins Plant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Molecular Weight:
7.7 | 4 (Arabidopsis thaliana)
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Hackett et al. (2017). An Organelle RNA Recognition Motif Protein Is Required for Photosystem II Subunit psbF Transcript Editing. Plant Physiol. 2017 Apr;173(4):2278-2293. doi: 10.1104/pp.16.01623.Levey et al. (2014). Expression of a nuclear-encoded psbH gene complements the plastidic RNA processing defect in the PSII mutant hcf107 in Arabidopsis thaliana. Plant J. 2014 Oct;80(2):292-304. doi: 10.1111/tpj.12632. Epub 2014 Sep 8.Verhoeven et al. (2009). Seasonal changes in abundance and phosphorylation status of photosynthetic proteins in eastern white pine and balsam fir. Tree Physiol. 29:361-374.
Special application note:
This product can be sold containing ProClin if requested
PIP2;3 is an aquaporin which facilitate the transport of water and small neutral molecules across cell membrane. Alternative names: plasma membrane intrinsic protein 2-3, OsPIP2;3
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Oryza sativa, Zea mays
Expected Species:
Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from Oryza sativa PIP2;3, UniProt: Q7XUA6
Antibody works very well on samples from roots. Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.
Application Details:
1 : 8000 (ELISA), 1 : 100 (IG), 1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to plasma membrane proteins AS09 505 | Anti-PIP1s | aquaporin, rabbit antibodiesAS09 507 | Anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1, rabbit antibodiesAS09 508 | Anti-PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5, rabbit antibodiesPlant protein extraction buffer
Molecular Weight:
31.9 | 26-29 kDa
Not reactive in:
Arabidopsis thaliana, Hordeum vulgare
Selected references:
Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.For immunogold technique, secondary antibodies conjugated with coloidal gold, 10 nm particle size were used.
PIP2;5 protein is an aquaporins which facilitates the transport of water and small neutral molecules across cell membrane.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Oryza sativa
Expected Species:
Sorghum bicolor, Zea mays Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from Oryza sativa PIP2-5 UniProt: Q8GRI8
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.
Application Details:
1 : 8000 (ELISA), 1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to plasma membrane proteins AS09 504 | Anti-PIP1;3 | aquaporin, plasma membrane intrinistic protein 1-3, rabbit antibodiesAS09 505 | Anti-PIP1s | aquaporin, rabbit antibodiesAS09 506 | Anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1, rabbit antibodiesAS09 507 | Anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1, rabbit antibodiesPlant protein extraction buffer
Molecular Weight:
29.7 | 26-29 kDa
Not reactive in:
Arabidopsis thaliana, Hordeum vulgare
Selected references:
Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
TIP2-1 belongs to aquaporin protein family and facilitates transport of water and small neutral solutes across tonoplast membrane.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Oryza sativa, Populus trichocarpa x deltoides, clone H11-11
Expected Species:
Gladiolus hybrid, Hordeum vulgare, Triticum aestivum, Zea mays Species of your interest not listed? Contact us
Immunogen:
KLH-cionjugated peptide derived from Oryza sativa TIP2-1, UniProt: Q7XA61
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.
collection of antibodies to tonoplast proteins AS09 492 | Anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma), rabbit antibodiesAS09 493 | Anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma), rabbit antibodiesAS09 494 | Anti-TIP2;1 (delta) | Delta-VM23, rabbit antibodiesAS09 510 | Anti-TIP2;1 | tonoplast intrinistic protein 2-1, rabbit antibodiesAS09 510 | Anti-TIP2;2 | tonoplast intrinistic protein 2-2. rabbit antibodiesAS09 511 | Anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal), rabbit antibodiesPlant protein extraction buffer
Molecular Weight:
24.8 | 20-24 kDa
Not reactive in:
Arabidopsis thaliana
Selected references:
Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
PsbP - 23 kDa extrinsic protein of photosystem II (PSII). Processing of the protein results in a protein with molecular mass of around 20 kDa. PsbP is required to optimize water splitting process in PSII, by probaböy by optimisation of calcium and Cl- levels. The protein is found in higher plants and algae but is not conserved in cyanobacteria. Synonymes:Oxygen-evolving enhancer protein 2-1, chloroplastic, OEE2, 23 kDa subunit of oxygen evolving system of photosystem II, OEC 23 kDa subunit, OEC23, 23 kDa thylakoid membrane protein
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
If you work with Chlamydomonas reinhardii, please use following PsbP antibody: AS06 142-23
Application Details:
1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
AS05 092 | Anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodiesAS06 142-33 | Anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodiesAS08 305 | Anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodiesAS06 142-16 | Anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodiesPlant protein extraction buffer Secondary antibodies | AgriseraSuperDeal
Pavlovič et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.Albanese et al. (2016). Isolation of novel PSII-LHCII megacomplexes from pea plants characterized by a combination of proteomics and electron microscopy. Photosynth Res. 2016 Jan 9.Grassl et al. (2012). Early events in plastid protein degradation in stay-green Arabidopsis reveal differential regulation beyond the retention of LHCII and chlorophyll. J. Proteome Res. October 2.Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).
Special application note:
This product can be sold containing ProClin if requested.
TIP2-1 belongs to aquaporin protein family and facilitates transport of water and small neutral solutes across tonoplast membrane.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Oryza sativa
Expected Species:
Arabidopsis thaliana, Hordeum vulgare, Populus trichocarpa, Ricinus communis, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from N-terminus of Oryza sativa TIP2-2, UniProt: Q5Z6F0
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.
Application Details:
1 : 8000 (ELISA), 1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to tonoplast proteins AS09 492 | Anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma), rabbit antibodiesAS09 493 | Anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma), rabbit antibodiesAS09 494 | Anti-TIP2;1 (delta) | Delta-VM23, rabbit antibodiesAS09 510 | Anti-TIP2;1 | tonoplast intrinistic protein 2-1, rabbit antibodiesAS09 511 | Anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal), rabbit antibodiesPlant protein extraction buffer
Molecular Weight:
25 | 20-24 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities. Plant Cell Physiol. 2008 Jan;49(1):30-9. Epub 2007 Nov 23.
Special application note:
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.
Lysine-tRNA ligase is located in chloroplast and involved in circadian rhytm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from Lysine-tRNA ligase protein sequence of Arabidopsis thaliana UniProt: Q39101, TAIR: AT3G01060
This protein is present in very low levels, therefore western blot conditions need to be adjusted by higher protein load/well and usage of a very sensitive detection system.
Application Details:
1 : 500 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
Photosynthetic antibody collectionPlant protein extraction buffer
Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid in 10mM Tris, 150 mM NaCl, 0.02% sodium azide
Storage Temp:
Store at 4°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Helianthus annuus , Nicotiana tabacum, Musa acuminata
Selected references:
Adhikari et al. (2018). Sulfate improves cadmium tolerance by limiting cadmium accumulation, modulation of sulfur metabolism and antioxidant defense system in maize. Environmental and Experimental Botany Volume 153, September 2018, Pages 143-162.Bastow et al. (2018). Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds. Plant Physiol. 2018 Jul;177(3):1267-1276. doi: 10.1104/pp.18.00478.Alché et al. (1998). Identification and immunolocalization of superoxide dismutase isoenzymes of olive pollen". Physiol. Plantarum 104, 772-776.
Special application note:
Total IgY concentration is 2.3 mg/ml.Reaction of the antibody to chloroplastic SOD isoform has not been determined yet.
Aconitase is a single subunit enzyme of the tricarboxylic acid cycle (or Krebs cycle) in the mitochondria. A cytosolic isoform is also part of the glyoxylate cycle. Aconitase catalyzes the dehydration / hydration of citrate to iso-citrate, via cis-aconitate as an intermediate. The reaction is facilitated by an iron-sulphur cluster in the active site of the enzyme. The iron-sulphur cluster is somewhat unstable, especially under oxidative stress, and loss of the cofactor leads to degradation of the protein.Alternative names: ACO, citrate hydro-lyase 1,2,3
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Host Animal:
Rabbit
Species Reactivity:
Arabidopsis thaliana ACO1,ACO2 and ACO3 isoforms, Brassica oleracea, Solanum lycopersicum
Expected Species:
Cucurbita maxima, Nicotiana tabacum, Ricinus communis, Solanum tuberosum, Vitis vinifera, Oryza sativa, Zea mays, Picea sitcHensis, Populus trichocarpa Species of your interest not listed? Contact us
Immunogen:
Arabidopsis ACO1 (AT4G35830, Q42560), codon 120 – 898 (C-terminus), was cloned in fusion with a N-terminal 6xHis tag, and over-expressed in E. coli. All recombinant protein accumulated in inclusion bodies, which were purified by centrifugation and solubilised in 6 M guanidine-HCl. The protein was refolded by dilution in 100 mM Tris-HCl 8.5, 10% (v/v) glycerol, 2 mM dithiothreitol, and concentrated prior to immunisation.
The antibody recognises all three Arabidopsis aconitase isoforms (ACO1, ACO2 and ACO3, see Bernard et al 2009). Possible differences in affinity have not been precisely quantified. Sensitivity threshold is between 2 and 10 ng for WB / ECL (see figure). Antibodies will recognize aconitase isoforms in denaturing and native gel electrophoresis.
Application Details:
1 : 5 000 -1 : 10 000 (WB). At higher concentrations the antibody binds aspecifically resulting in non-specific signals around 60 kDa, including Rubisco subunits.
Purity:
Serum
Reconstitution:
For reconstitution add 100 µl of sterile water.
Related products:
collection of antibodies to proteins located in mitochondriaPlant protein extraction bufferSecondary antibodies | AgriseraSuperDeal
Molecular Weight:
98 kDa. Note that ACO1, ACO2 and ACO3 cannot be distinguished in size by standard SDS-PAGE.
Not reactive in:
Chlamydomonas reinhardii, cyanobacteria
Selected references:
Rurek et al. (2018). Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought Involves Impaired Coordination of Transcriptomic and Proteomic Response and Regulation of Various Multifunctional Proteins. Preprints 2018, 2018010276 (doi: 10.20944/preprints201801.0276.v1).Setién et al. (2014). Root phosphoenolpyruvate carboxylase and NAD-malic enzymes activity increase the ammonium-assimilating capacity in tomato. J Plant Physiol. 171:49-63.Birke et al. (2012). Cysteine biosynthesis, in concert with a novel mechanism, contributes to sulfide detoxification in mitochondria of Arabidopsis thaliana. Biochem J. May 2, ahead of print.
Special application note:
Arabidopsis expresses three highly similar aconitase isozymes (ACO1/ AT4G35830, ACO2/AT4G26970 and ACO3/AT2G05710), of which ACO1 is the cytosolic isoform, while ACO2 and ACO3 are predominantly located in the mitochondria (Arnaud et al 2007, Bernard et al 2009). The combined abundance and activity of the mitochondrial aconitases is about 3 times higher than the cytosolic pool (Bernard et al 2009).The Arabidopsis isoforms are more similar in amino acid sequence to mammalian iron-regulatory proteins (IRP-1) than to the mammalian and yeast mitochondrial aconitases.
Ascorbate peroxidase (APX) is the enzyme catalyzing the ascorbate-dependent reduction of hydrogen peroxide. Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in fruits and photosynthetic tissues. AA is found in every compartment of the plant cell including the apoplast.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized in PBS pH 7.4
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Brassica. juncea, Citrus sinensis, Fragaria ananassa, Gossypium hirsutum, Solanum tuberosum, Sorghum bicolor, Pinus pinaster, Vitis vinifera Species of your interest not listed? Contact us
Immunogen:
KLH-conjugated peptide derived from N-terminal of Zea mays cytosolic APX UniProt: Q41772
Applications:
Immunoprecipitation (IP), Immunolocalization (IL), Western blot (WB)
Bègue et al. (2019). CDC48 regulates ascorbate peroxidase in tobacco. J Exp Bot. 2019 Mar 1. pii: erz097. doi: 10.1093/jxb/erz097.Wang et al. (2018). Resistance protein Pit interacts with the GEF OsSPK1 to activate OsRac1 and trigger rice immunity. Proc Natl Acad Sci U S A. 2018 Nov 16. pii: 201813058. doi: 10.1073/pnas.1813058115. Balážová et al. (2018). Zinc oxide nanoparticles phytotoxicity on halophyte from genus Salicornia. Plant Physiol Biochem. 2018 Sep;130:30-42. doi: 10.1016/j.plaphy.2018.06.013.Adhikari et al. (2018). Sulfate improves cadmium tolerance by limiting cadmium accumulation, modulation of sulfur metabolism and antioxidant defense system in maize. Environmental and Experimental Botany Volume 153, September 2018, Pages 143-162.Ferrer et al. (2018). Differential Pb tolerance in metallicolous and non-metallicolous Zygophyllum fabago populations involves the strengthening of the antioxidative pathways. Environ & Exp Botany, Vo. 150, June 2018, Pages 141-151.Aroca et al. (2015). S-sulfhydration: a new post-translational modification in plant systems. Plant Physiology March 2015 pp.00009.2015.Terrile et al. (2014). Nitric oxide-mediated cell death is triggered by chitosan in F. eumartii spores. Pest Manag Sci. 2014 Apr 25. doi: 10.1002/ps.3814.Tsaniklidis et al. (2013). L-Ascorbic acid metabolism in parthenocarpic and seeded cherry tomatoes. Plant Growth Regul,DOI 10.1007/s10725-013-9845-0. (Solanum lycopersicum, immunolocalization)
Glutathione reductase (GR, EC 1.6.4.2) is an important enzyme for plant protection against environmental stress. It functions in plant defense reactions in the conversion of glutathione disulphide to reduced glutathione (GSH).
