p75NTR (CD271) was originally discovered as a low affinity nerve growth factor receptor (NGFR). Later it was found that it was the receptor for all neurotrophins, including NGF, BDNF, NT3 and NT4/5. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. Recent research proposes the extracellular domain of p75NTR as a biomarker for monitoring the progression of motor neuron disease (MND), also known as Amyotrophic Lateral Sclerosis (ALS) or Lou Gehrig's Disease. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid antibody (1 mg/mL) in PBS, pH 7.2-7.6, without preservative. Typical Fluorophore/Protein (F/P) - ratio is 3-10.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.
Applications:
FC,ICC
Clone number:
8J2
Antibody Isotype:
IgG2a
Application Details:
Immunocytochemistry: 1-5 µg/mL. Light fixation only, or unfixed works best. Epitope is sensitive to fixation. Flow Cytometry: 5-20 µg/mL. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Matusica D, Fenech MP, Rogers ML, Rush RA. (2008) Characterisation and use of the NSC-34 cell liner for study of nerurotrophin receptor trafficking. <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/17896795?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum"> J. Neurosci. Res. 86(3) pp. 553-65. Huh CY, Danik M, Manseau F, Trudeau LE, Williams S. (2008) Chronic exposure to nerve growth factor increases acetylcholine and glutamate release from cholinergic neurons of the rat medial septum and diagonal band of Boca via mechanisms mediated bu p75NTR.<a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/18256260?ordinalpos=3&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum"> J. Neurosci. 28(6) pp. 1404-9. Lagares A, Li HY, Zhou XF, Avendano C. (2007) Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio-neuronal precursor maturation. <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/17652585?ordinalpos=6&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum"> J. Neurosci. 27(30) pp. 7939-53.
Specificity:
Human, reacts with human, mouse and rat. Cross-reactivity with other species not tested but expected.This antibody is specific for NGFR/p75NTR as demonstRated by western blotting and immunprecipitation. The antibody recognizes extracellular p75NTR under non-reducing conditions.
Purification:
Protein A purified IgG was labelled with FITC and free dye removed by gel filtration.
p75NTR (CD271) was originally discovered as a low affinity nerve growth factor receptor (NGFR). Later it was found that it was the receptor for all neurotrophins, including NGF, BDNF, NT3 and NT4/5. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. Recent research proposes the extracellular domain of p75NTR as a biomarker for monitoring the progression of motor neuron disease (MND), also known as Amyotrophic Lateral Sclerosis (ALS) or Lou Gehrig's Disease. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid antibody (1 mg/mL) in PBS, pH 7.2-7.6, without preservative. Typical Fluorophore/Protein (F/P) - ratio is 3-10.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.
Applications:
FC,ICC
Clone number:
8J2
Antibody Isotype:
IgG2a
Application Details:
Immunocytochemistry: 1-5 µg/mL. Light fixation only, or unfixed works best. Epitope is sensitive to fixation. Flow Cytometry: 5-20 µg/mL. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Matusica D, Fenech MP, Rogers ML, Rush RA. (2008) Characterisation and use of the NSC-34 cell liner for study of nerurotrophin receptor trafficking. <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/17896795?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum"> J. Neurosci. Res. 86(3) pp. 553-65. Huh CY, Danik M, Manseau F, Trudeau LE, Williams S. (2008) Chronic exposure to nerve growth factor increases acetylcholine and glutamate release from cholinergic neurons of the rat medial septum and diagonal band of Boca via mechanisms mediated bu p75NTR.<a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/18256260?ordinalpos=3&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum"> J. Neurosci. 28(6) pp. 1404-9. Lagares A, Li HY, Zhou XF, Avendano C. (2007) Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio-neuronal precursor maturation. <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/17652585?ordinalpos=6&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum"> J. Neurosci. 27(30) pp. 7939-53.
Specificity:
Human, reacts with human, mouse and rat. Cross-reactivity with other species not tested but expected.This antibody is specific for NGFR/p75NTR as demonstRated by western blotting and immunprecipitation. The antibody recognizes extracellular p75NTR under non-reducing conditions.
Purification:
Protein A purified IgG was labelled with ATTO 488 and free dye removed by gel filtration.
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display sevvere movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Chicken,Human,Rat
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human (aa: 139-149), mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Zouh et al. (1996) Mol. Neurobio. 13(3): 185-97 Rush et al. (1997) Clin. Exp. Pharmacol. 24: 549-55 Tafreshi et al. (1998) Neurosci. 83(2): 373-80
Specificity:
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide (C-ELLDEDQKVRPNEE) as a part of human BDNF precursor protein (aa: 69-82) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC, WB. 1-5 µg/mL is recommended for both applications, Flow Cytometry (2?g/10^6 cells). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A Acheson et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7700353'>(1995) Nat. 74: 450-3 Q Yan et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8083736'>(1994) J. Neurosci. 14(9): 5281-91 XF Zhou et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8895863'>(1996) Neurosci. 74: 945-53 XF Zhou, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9454633'>(1998) Exp. Neurol. 149: 237-42 B Mellstrom et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15581398'>(2004) Crit Rev Neurobiol 16, 43-9 I Tapia-Arancibia et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15571756'>(2004) Front Neuroendocrinol 25, 77-107 S Pezet, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12589922'> (2002) Brain Res Brain Res Rev 40, 240-9 Barde Y. A. et al (1989) EMBO J. 1: 549 Conner J et al. (1997) J. Neurosci. 17: 2295 L Ulman et al http://www.ncbi.nlm.nih.gov/pubmed/18971468'> (2008) J Neurosci. Oct 29;28(44)
Specificity:
Used in western blot, this antiserum detects a 35 kDa band corresponding to the molecular weight of proBDNF. No cross reactivity with other proneurotrophins was detected. This antibody is known to react with human, mouse and rat proBDNF and also expected to recognise other mammalian proBDNF.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human mature BDNF expressed in E.coli
Applications:
ELISA,WB
Clone number:
4C8
Antibody Isotype:
IgG
Application Details:
ELISA: 50-100 ng/mL Western Blotting (denaturing and reducing): 0.2 to 2 µg/mL. M-1744-100 antibody detects 14 kDa mature BDNF monomer in human serum human and acid treated human and rodent whole tissue homogenates. In simple Tris-homogenate human brain lysates, M-1744-100 detects mature BDNF at 14kDa as well as an uncharacterized band at ~50kDa. No proBDNF band is detected in our samples, although M-1744-100 will detect proBDNF in purified form on blots. Additionally, M-1744-100 detects an additional band 18 kDa BDNF isoform human serum. For cell lysates, some caution is advised as in our hands M-1744-100 fails to detect mature BDNF on SH-SY5Y or C6 cell lysates but does detect mature BDNF in neuronal cell culture lysates. The reason for these differences has not been characterized. For detection of mature BDNF in cell lysates such as SH-SY5Y we recommend affinity-purified rabbit polyclonal antibody to rhBDNF (R-1707-100), or rabbit polyclonal antibody to BDNF peptide 1-10 (R-083-100, whole serum; R-066-500, IgG). Immunofluorescence: 1 to 4 µg/mL, 4% formaldehyde fixation and permeabilization. Staining can be weak until optimized. M-1744-100 is not recommended for Flow Cytometry at this time. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human mature BDNF expressed in E.coli
Applications:
ELISA,ICC,WB
Clone number:
3C11
Antibody Isotype:
IgG
Application Details:
ELISA (50-100 ng/mL), WB (0.2 to 2 µg/mL), IF (2 to 20 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display sevvere movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Chicken,Human,Rat
Immunogen:
Recombinant human NT3
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA, WB, dot blot, inhibition of biological activity. A dilution of 1:200 to 1:2000 is recommended for IHC, ELISA and western blot. For inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Zouh et al. (1996) Mol. Neurobio. 13(3): 185-97 Rush et al. (1997) Clin. Exp. Pharmacol. 24: 549-55 Tafreshi et al. (1998) Neurosci. 83(2): 373-80
Specificity:
A cross reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by 1-site ELISA. This antiserum is known to react with rat, chicken and human NT3.
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display sevvere movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Chicken,Human,Rat
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human, mouse and rat NT3 protein conjugated to BSA
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Zouh et al. (1996) Mol. Neurobio. 13(3): 185-97 Rush et al. (1997) Clin. Exp. Pharmacol. 24: 549-55 Tafreshi et al. (1998) Neurosci. 83(2): 373-80
Specificity:
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display sevvere movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Chicken,Human,Rat
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human, mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A dilution of 1:500 to 1:2000 is recommended for IHC, western blot. For inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Zouh et al. (1996) Mol. Neurobio. 13(3): 185-97 Rush et al. (1997) Clin. Exp. Pharmacol. 24: 549-55 Tafreshi et al. (1998) Neurosci. 83(2): 373-80
Specificity:
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Ebendal, T. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2468788'> (1989) J Neurosci Res 22, 223-240. Zhou, X. F. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7815824'> (1994) J Neurosci Methods 54, 95-102. Angeletti, P. U. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=4880217'> (1968) Adv Enzymol Relat Areas Mol Biol 31, 51-7 Hesse K. et al. http://www.ncbi.nlm.nih.gov/pubmed/9291146'> (1997) Neurosci Lett. Aug 8;231(2):83- Miao J et al. http://www.ncbi.nlm.nih.gov/pubmed/23000062'> (2012) Neurosci Res. Dec;74(3-4):269-7
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Ebendal, T. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2468788'> (1989) J Neurosci Res 22, 223-240. Zhou, X. F. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7815824'> (1994) J Neurosci Methods 54, 95-102. Angeletti, P. U. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=4880217'> (1968) Adv Enzymol Relat Areas Mol Biol 31, 51-7 Hesse K. et al. http://www.ncbi.nlm.nih.gov/pubmed/9291146'> (1997) Neurosci Lett. Aug 8;231(2):83- Miao J et al. http://www.ncbi.nlm.nih.gov/pubmed/23000062'> (2012) Neurosci Res. Dec;74(3-4):269-7
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A dilution of 1:1000-1:5000 is recommended for IHC, western blot and immunoblot; 1:15000 for ELISA; for inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Ebendal, T. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2468788'> (1989) J Neurosci Res 22, 223-240. Zhou, X. F. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation_uids=7815824'> (1994) J Neurosci Methods 54, 95-102. Angeletti, P. U. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=4880217'> (1968) Adv Enzymol Relat Areas Mol Biol 31, 51-7 Hesse K. et al. http://www.ncbi.nlm.nih.gov/pubmed/9291146'> (1997) Neurosci Lett. Aug 8;231(2):83- Miao J et al. http://www.ncbi.nlm.nih.gov/pubmed/23000062'> (2012) Neurosci Res. Dec;74(3-4):269-7
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
Sheep anti-Myelin basic protein (MBP) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of approximately 80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP). MBP is an 'intrinsically unstructured' protein with a high proportion (approximately 75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system. A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational. Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic. The message for myelin basic protein is selectively translocated to the ends of the cell processes. Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model. FUNCTION: Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity). SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Myelin membrane; peripheral membrane protein; cytoplasmic side. Cytoplasmic side of myelin. TISSUE SPECIFICITY: Found in both the central and the peripheral nervous system. PTM: At least 5 charge isomers; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4 and C5 (the least cationic form); are produced as a result of optional posttranslational modifications such as phosphorylation of serine or threonine residues, deamidation of glutamine or asparagine residues, citrullination and methylation of arginine residues. C1 and C2 are unphosphorylated, C3 and C4 are monophosphorylated and C5 is phosphorylated at two positions. SIMILARITY: Belongs to the myelin basic protein family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Guinea Pig,Human,Rat
Immunogen:
A synthetic peptide (YG SLPQKSQRSQ DENPVV, aa: 68-86) as part of guinea pig MBP protein conjugated to KLH
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1:4000 is recommended. Immunostaining for MBP of abnormal appearing oligodendrocytic process and cell bodies in demyelinating areas. This antibody recognises only areas of myelin degeneration when tested in injured spinal cord and lesioned sciatic nerves. It also stains discrete white matter in the brain of multiple system atrophy. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Myelin Basic Protein
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Schwartz, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12440382'>Prog Brain Res 137, 401-6 (2002) Hauben, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve=PubMed&dopt=Citation&list_uids=11518733'>J Clin Invest 108, 591-9 (Aug, 2001) Yoles, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11356861'>J Neurosci 21, 3740-8 (Jun 1, 2001) Hauben, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10964948'>J Neurosci 20, 6421-30 (Sep 1, 2000) Harauz, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15219899'>Nature 389, 783-4 (1997). Micron 35, 503-42 (2004) Givogri, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10650873'>J Neurosci Res 59, 153-9 (Jan 15, 2000) Kim, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9251242'>Int J Biochem Cell Biol 29, 743-51 (May, 1997) Kalwy, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7522797'>Mol Membr Biol 11, 67-78 (Apr-Jun, 1994) Wajgt, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6198864'>Acta Neurol Scand 68, 337-43 (Nov, 1983) Day, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2418054'>J Neuroimmunol 10, 289-312 (Feb, 1986) 1Mikoshiba, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1709079'>Comp Biochem Physiol C 98, 51-61 (1991) 1Brophy, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7509522'>Trends Neurosci 16, 515-21 (Dec, 1993) 1Matsuo, A. et al. (1997) Am. J. Pathol. 150(4): 1253-1266
Specificity:
This antiserum recognizes MBP in demyelinated nerve tissues. Immunohistochemical analysis of lesioned rat spinal cord indictaes a high level of specificity for this antiserum. This antiserum reacts with human and rat MBP.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human BDNF
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, Inhibition of biological activity in vitro/in vivo. Recommended to be used at a dilution of 1:200-2000 for immunohistochemistry on Zamboni's fixed frozen tissue; not recommended for formalin fixed paraffin embedded tissues. 1:10 to 1:50 for inhibition of biological activity in vitro. Use neat for in vivo studies at 5-10 µL/g body weight. Whole serum format will caused immune responses, purified format is preferred for most in vivo work. Not recommended for western blots. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A Acheson et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7700353'>(1995) Nat. 74: 450-3 Q Yan et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8083736'>(1994) J. Neurosci. 14(9): 5281-91 XF Zhou et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8895863'>(1996) Neurosci. 74: 945-53 XF Zhou, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9454633'>(1998) Exp. Neurol. 149: 237-42 B Mellstrom et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15581398'>(2004) Crit Rev Neurobiol 16, 43-9 I Tapia-Arancibia et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15571756'>(2004) Front Neuroendocrinol 25, 77-107 S Pezet, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12589922'> (2002) Brain Res Brain Res Rev 40, 240-9 JA Coull et al http://www.ncbi.nlm.nih.gov/pubmed/16355225'> (2005) Nature. Dec 15;438(7070):1017-21. C Gomes et al http://www.ncbi.nlm.nih.gov/pubmed/23363775'> (2013) J Neuroinflammation. Jan 30;10:16.
Specificity:
A cross reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by one site ELISA. This antiserum recognises BDNF from rat, mouse and human.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
Recombinant human BDNF
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, Inhibition of biological activity in vitro/in vivo, ELISA. Recommended to be used at an amount of 1-10 µg/mL for immunohistochemistry on Zamboni's fixed, frozen tissue. Not recommended for paraffin embedded tissues. Primary use is for biological activity in vitro and in vivo. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antibody does not react to BDNF in western blot, thus western blot is not a recommended application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Vissio P.G. et al (2008) Brain-derived neurotrophic factor (BDNF)-like immunoreactivity localization in the retina and brain of Cichlasoma dimerus (Teleostei, Perciformes) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18343472'>Tissue Cell. 2008 Aug;40(4):261-70. A Acheson et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7700353'>(1995) Nat. 74: 450-3 Q Yan et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8083736'>(1994) J. Neurosci. 14(9): 5281-91 XF Zhou et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8895863'>(1996) Neurosci. 74: 945-53 XF Zhou, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9454633'>(1998) Exp. Neurol. 149: 237-42 B Mellstrom et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15581398'>(2004) Crit Rev Neurobiol 16, 43-9 I Tapia-Arancibia et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15571756'>(2004) Front Neuroendocrinol 25, 77-107 S Pezet, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12589922'> (2002) Brain Res Brain Res Rev 40, 240-9 JA Coull et al http://www.ncbi.nlm.nih.gov/pubmed/16355225'> (2005) Nature. Dec 15;438(7070):1017-21. C Gomes et al http://www.ncbi.nlm.nih.gov/pubmed/23363775'> (2013) J Neuroinflammation. Jan 30;10:16.
Specificity:
A cross reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by one site ELISA. Known to react with BDNF from rat and human.
Fibroblast growth factors (FGFs), a heparin binding growth factor, exhibit widespread mitogenic and neurotrophic activities in a variety of different cells including mesenchymal, neuroectodermal and endothelial cells. aFGF (FGF-1) and bFGF (FGF-2) are present in relatively high levels in CNS. aFGF is expressed by a subset of neuronal populations, while bFGF is expressed by astrocytes, both lack signal peptides. Human bFGF is a 17.2 kDa protein containing 155 amino acid residues. FUNCTION: The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. SUBUNIT: Monomer. Interacts with CSPG4 and FGFBP1. Found in a complex with FGFBP1, FGF1 and FGF2. MISCELLANEOUS: This protein binds heparin more strongly than does aFGF. SIMILARITY: Belongs to the heparin-binding growth factors family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human basic FGF
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC (frozen), WB. Recommended to be used at a dilution of 1: 1000 to 1:2000 for both applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Abraham, et al. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2425435'>(1986) Science. 233(4763):545-8 Kurokawa, et al. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2435575'>(1987) FEBS Lett. 213(1):189-94 Bieger, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7891078'>(1995) J Neurochem 64, 1521-7 Takayama, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=758495'>(1995)Nat Med 1, 53-8. Eckenstein http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7852998'> (1994) J Neurobiol 25, 1467-80. (1994) Abraham, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3780670'> (1986) Embo J 5, 2523-8 English, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7750657'> (1995)Dev Biol 169, 57-6
Specificity:
A high level of specificity for bFGF was shown by immunohistochemistry for this antiserum. This antibody is known to react with human, mouse and rat basic FGF.
DBH is an oxireductase belonging to the copper type II ascorbate-dependent monooxygenase family. DBH exists as a homotetramer composed of two non-covalently bound disulfide-linked dimers. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It binds 2 copper ions and 1 PQQ per subunit . Depending on the presence of a signal peptide, DBH can exist in both soluble and membrane-bound forms.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Bovine,Guinea Pig,Human,Rat
Immunogen:
Native enzyme prepared from Bovine adrenal glands. Purity was greater than 95% by PAGE.
Applications:
IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC (frozen), Western Blot, radioimmunoassay and inhibition of enzymatic activity. Recommended to be used at a dilution of 1:500 to 1: 2000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rush R. A. et al (1980) Gut Rev clin Lab Sci 12: 241 Verndell I. M. et al (1982) J Histochem Cytochem 30: 682
Specificity:
This antiserum has been extensively characterised in several laboratories. It reacts with a single band on western blot of bovine adrenal homogenates and stains only cells known to contain DBH, such as sympathetic neurons, adrenal medullary cells and central adrenergic neurons. Staining is abolished by preincubation with the enzyme. This antibody is known to react with bovine, human, guinea pig and rat DBH.
Rabbit anti-Myelin basic protein (MBP) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of approximately 80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP). MBP is an 'intrinsically unstructured' protein with a high proportion (approximately 75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system. A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational. Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic. The message for myelin basic protein is selectively translocated to the ends of the cell processes. Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model. FUNCTION: Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity). SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Myelin membrane; peripheral membrane protein; cytoplasmic side. Cytoplasmic side of myelin. TISSUE SPECIFICITY: Found in both the central and the peripheral nervous system.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Guinea Pig,Human,Rat
Immunogen:
A synthetic peptide (YG SLPQKSQRSQ DENPVV, aa: 68-86) as part of guinea pig MBP protein conjugated to KLH
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1:4000 is recommended. Immunostaining for MBP of abnormal appearing oligodendrocytic process and cell bodies in demyelinating areas. This antibody recognises only areas of myelin degeneration when tested in injured spinal cord and lesioned sciatic nerves. It also stains discrete white matter in the brain of multiple system atrophy. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Myelin Basic Protein
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Schwartz, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12440382'>Prog Brain Res 137, 401-6 (2002) Hauben, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve=PubMed&dopt=Citation&list_uids=11518733'>J Clin Invest 108, 591-9 (Aug, 2001) Yoles, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11356861'>J Neurosci 21, 3740-8 (Jun 1, 2001) Hauben, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10964948'>J Neurosci 20, 6421-30 (Sep 1, 2000) Harauz, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15219899'>Nature 389, 783-4 (1997). Micron 35, 503-42 (2004) Givogri, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10650873'>J Neurosci Res 59, 153-9 (Jan 15, 2000) Kim, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9251242'>Int J Biochem Cell Biol 29, 743-51 (May, 1997) Kalwy, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7522797'>Mol Membr Biol 11, 67-78 (Apr-Jun, 1994) Wajgt, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6198864'>Acta Neurol Scand 68, 337-43 (Nov, 1983) Day, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2418054'>J Neuroimmunol 10, 289-312 (Feb, 1986) 1Mikoshiba, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1709079'>Comp Biochem Physiol C 98, 51-61 (1991) 1Brophy, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7509522'>Trends Neurosci 16, 515-21 (Dec, 1993) 1Matsuo, A. et al. (1997) Am. J. Pathol. 150(4): 1253-1266
Specificity:
This antiserum recognizes MBP in demyelinated nerve tissues. Immunohistochemical analysis of lesioned rat spinal cord indictaes a high level of specificity for this antiserum. This antiserum reacts with human and rat MBP.
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Primate,Rat
Immunogen:
Recombinant human NT4
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA (1 site), Western Blot, ICC, inhibition of biological activity in vitro/in vivo. Recommended to be used at a concentration of 1-10 µg/mL for immunohistochemistry, ELISA, ICC and Western blot and inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). Note that the concentration of NT4 is generally low in most tissues nevertheless, neonatal testes of rat can be used as a good positive control. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Lodouichi (2000) J. Neuroscie. 20(6): 2155 Zhang et al. (1999) J Neurosci. Meths. 89 (1): 69
Specificity:
Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by dot blot. Known to react with NT4 from rat and human, mouse and monkey.
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Primate,Rat
Immunogen:
Recombinant human NT4
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA (1 site), Western Blot, inhibition of biological activity in vitro/in vivo. Recommended to be used at a dilution of 1:500 to 1:2000 for immunohistochemistry, ELISA and Western blot. 1:10 to 1:50 for inhibition of biological activity in vitro. Use neat for in vivo studies at 5-10 µL/g body weight. Note that the concentration of NT4 is generally low in most tissues nevertheless, neonatal testes of rat can be used as a good positive control. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Lodouichi (2000) J. Neuroscie. 20(6): 2155 Zhang et al. (1999) J Neurosci. Meths. 89 (1): 69
Specificity:
Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by 1-site ELISA. Known to react with NT4 from rat and human, mouse and monkey.
FUNCTION: Seems to promotes the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS with no preservatives.
Host Animal:
Rabbit
Species Reactivity:
Chicken,Human,Rat
Immunogen:
Recombinant human NT3
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Zhou et al. (1996) Mol. Neurobio. 13(3): 185-97 Rush et al. (1997) Clin. Exp. Pharmacol. 24: 549-55 Tafreshi et al. (1998) Neurosci. 83(2): 373-80
Specificity:
A cross reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
FUNCTION: Seems to promotes the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Chicken,Human,Rat
Immunogen:
Recombinant human NT3
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A dilution of 1:500 to 1:1000 is recommended for IHC, ELISA and western blot. For inhibition of biological activity: 1:10-50 for in vitro, 2-10 µL/g body weight for in vivo. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Zouh et al. (1996) Mol. Neurobio. 13(3): 185-97 Rush et al. (1997) Clin. Exp. Pharmacol. 24: 549-55 Tafreshi et al. (1998) Neurosci. 83(2): 373-80
Specificity:
A cross reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
FUNCTION: Seems to promote the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Chicken,Human,Rat
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human (aa: 139-149), mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A dilution of 1:500 to 1:2000 is recommended for IHC, western blot. For inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Zouh et al. (1996) Mol. Neurobio. 13(3): 185-97 Rush et al. (1997) Clin. Exp. Pharmacol. 24: 549-55 Tafreshi et al. (1998) Neurosci. 83(2): 373-80
Specificity:
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
FUNCTION: Seems to promotes the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Chicken,Human,Rat
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human (aa: 139-149), mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Zouh et al. (1996) Mol. Neurobio. 13(3): 185-97 Rush et al. (1997) Clin. Exp. Pharmacol. 24: 549-55 Tafreshi et al. (1998) Neurosci. 83(2): 373-80
Specificity:
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.2-7.6 without preservatives
Host Animal:
Rabbit
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antibody was tested on cultured sensory neurons supported by 100 ng/mL of purified mouse beta NGF. Be advised that 2 µg/mL will neutralize 100 ng/mL of mouse NGF. The higher 10 µg/mL is only recommended if the concentration of NGF being used is higher than 100 ng/mL such as the 200 or 500ng/mL that is occasionally used in some culture systems. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Cassina P. et al (2005) Astrocyte activation by fibroblast growth factor-1 and motor neuron apoptosis: implications for amyotrophic lateral sclerosis. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15773903'> J Neurochem. 2005 Apr;93(1):38-46. Zhou, X. F. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7815824'> (1994) J Neurosci Methods 54, 95-102. Angeletti, P. U. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=4880217'> (1968) Adv Enzymol Relat Areas Mol Biol 31, 51-7 Ebendal, T. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2468788'> (1989) J Neurosci Res 22, 223-240. Hesse K. et al. http://www.ncbi.nlm.nih.gov/pubmed/9291146'> (1997) Neurosci Lett. Aug 8;231(2):83- Miao J et al. http://www.ncbi.nlm.nih.gov/pubmed/23000062'> (2012) Neurosci Res. Dec;74(3-4):269-7
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. DOMAIN: Death domain is responsible for interaction with RANBP9. PTM: N- and O-glycosylated. PTM: O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. PTM: Phosphorylated on serine residues. SIMILARITY: Contains 1 death domain. SIMILARITY: Contains 4 TNFR-Cys repeats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
Extra cellular domain of human p75NTR
Applications:
ICC,IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC, immunofluorescence. Recommended to be used at a dilution of 1:500 to 1:2000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human BDNF
Applications:
IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA (1 site), Western Blot, inhibition of biological activity in vitro/in vivo. Recommended to be used at a dilution of 1:1000 for immunohistochemistry, ELISA and Western blot. 1:10 to 1:50 for inhibition of biological activity in vitro. Use neat for in vivo studies at 5-10 uL/g body weight. This antiserum stains cell bodies and some nerve terminals in the dorsal horn of the rat spinal cord, however, does not stain finest nerve terminals. Western Blotting: Antibody does detect BDNF forms in tissue lysates but there are multiple bands present, many of which are uncharacterized. The antibody detects 14 kDa (mature BDNF), 32 kDa (proBDNF) and a 18 kDa BDNF isoform (see blot examples). In cell lysates, only 18 kDa and 32 kDa BDNF are detected. The reason for these differences has not been characterized. Alternative antibodies for Western Blotting are: R-017-500 (IgG-purified form of R-088-100 for tissue homogenate analysis); R-1707-100 (cell lysates and tissue homogenates), R-083-100/R-066-500 (cell lysates, tissue homogenates and human serum); M-1744-50/100 (human serum and tissue homogenates). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A Acheson et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7700353'>(1995) Nat. 74: 450-3 Q Yan et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8083736'>(1994) J. Neurosci. 14(9): 5281-91 XF Zhou et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8895863'>(1996) Neurosci. 74: 945-53 XF Zhou, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9454633'>(1998) Exp. Neurol. 149: 237-42 B Mellstrom et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15581398'>(2004) Crit Rev Neurobiol 16, 43-9 I Tapia-Arancibia et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15571756'>(2004) Front Neuroendocrinol 25, 77-107 S Pezet, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12589922'> (2002) Brain Res Brain Res Rev 40, 240-9 Barde Y. A. et al (1989) EMBO J. 1: 549 Conner J et al. (1997) J. Neurosci. 17: 2295 JA Coull et al http://www.ncbi.nlm.nih.gov/pubmed/16355225'> (2005) Nature. Dec 15;438(7070):1017-21. 1C Gomes et al http://www.ncbi.nlm.nih.gov/pubmed/23363775'> (2013) J Neuroinflammation. Jan 30;10:16.
Specificity:
Less than 0.1% cross-reactivity against NGF, NT3 and NT4/5 by dot blot. Known to react with BDNF from rat, mouse and human. Expected to react with BDNF from other species due to amino acid sequence homology.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide (C-ELLDEDQKVRPNEE) as a part of human BDNF precursor protein (aa: 69-82) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:1000 to 1:5000 is recommended for both applications. ICC: 1:500 to 1:2000, antibody works on 4% formaldehyde fixed cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A Acheson et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7700353'>(1995) Nat. 74: 450-3 Q Yan et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8083736'>(1994) J. Neurosci. 14(9): 5281-91 XF Zhou et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8895863'>(1996) Neurosci. 74: 945-53 XF Zhou, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9454633'>(1998) Exp. Neurol. 149: 237-42 B Mellstrom et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15581398'>(2004) Crit Rev Neurobiol 16, 43-9 I Tapia-Arancibia et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15571756'>(2004) Front Neuroendocrinol 25, 77-107 S Pezet, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12589922'> (2002) Brain Res Brain Res Rev 40, 240-9 Barde Y. A. et al (1989) EMBO J. 1: 549 Conner J et al. (1997) J. Neurosci. 17: 2295 L Ulman, et al http://www.ncbi.nlm.nih.gov/pubmed/18971468'> (2008) J Neurosci. 2008 Oct 29;28(44):11263-8.
Specificity:
Used in western blot, this antiserum detects a 35 kDa band corresponding to the molecular weight of proBDNF. No cross reactivity with other proneurotrophins was detected. This antiserum is known to react with human, mouse and rat proBDNF and also expected to recognise other mammalian proBDNF.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A dilution of 1:1000-1:5000 is recommended for IHC, western blot and immunoblot; 1:15000 for ELISA; for inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Ebendal, T. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2468788'> (1989) J Neurosci Res 22, 223-240. Zhou, X. F. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation_uids=7815824'> (1994) J Neurosci Methods 54, 95-102. Angeletti, P. U. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=4880217'> (1968) Adv Enzymol Relat Areas Mol Biol 31, 51-7 Hesse K. et al. http://www.ncbi.nlm.nih.gov/pubmed/9291146'> (1997) Neurosci Lett. Aug 8;231(2):83- Miao J et al. http://www.ncbi.nlm.nih.gov/pubmed/23000062'> (2012) Neurosci Res. Dec;74(3-4):269-7
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF bot not bovine NGF.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst primates.
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting: This antibody detects multiple BDNF isoforms (14 kDa mature BDNF, 18 kDa isoform, 28 kDa BDNF dimer/truncated BDNF, 32 kDa proBDNF monomer) depending on sample application (human serum, cell lysate, tissue homogenate). Antibody also detects BDNF under non-reducing conditions (McLean NA, 2014). ELISA: Detection only, 1:1000-1:5000 recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A Acheson et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7700353'>(1995) Nat. 74: 450-3 Q Yan et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8083736'>(1994) J. Neurosci. 14(9): 5281-91 XF Zhou et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8895863'>(1996) Neurosci. 74: 945-53 XF Zhou, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9454633'>(1998) Exp. Neurol. 149: 237-42 B Mellstrom et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15581398'>(2004) Crit Rev Neurobiol 16, 43-9 I Tapia-Arancibia et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15571756'>(2004) Front Neuroendocrinol 25, 77-107 S Pezet, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12589922'> (2002) Brain Res Brain Res Rev 40, 240-9 Barde Y. A. et al (1989) EMBO J. 1: 549 Conner J et al. (1997) J. Neurosci. 17: 2295 JA Coull et al http://www.ncbi.nlm.nih.gov/pubmed/16355225'> (2005) Nature. Dec 15;438(7070):1017-21. 1C Gomes et al http://www.ncbi.nlm.nih.gov/pubmed/23363775'> (2013) J Neuroinflammation. Jan 30;10:16.
Specificity:
Less than 0.1% cross reactivity with mouse NGF, recombinant human NT3 and NT4/5 has been recorded by dot blot analysis. This antiserum is known to recognise rat, mouse and human BDNF, and is expected to react with BDNF from other species due to amino acid sequence homology.
Rabbit anti-Vanilloid receptor-like protein 1 (VRL-1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
TISSUE SPECIFICITY: Ubiquitously expressed. Expressed in dorsal root ganglia, trigeminal ganglia, spinal chord (Lissauer's tract, dorsal horn and dorsal columns) (at protein level). PTM: N-glycosylated. PTM: Phosphorylated by PKA. SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (C-KNSASEEDHLPLQVLQSP) of rat VRL-1 protein (aa: 744-761) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, Immunofluorescence, Western blot. Recommended to be used at a dilution of 1: 1000 to 1: 2000 for immunohistochemistry and Western blot, for Immunofluorescence at a dilution of 1:50 to 1: 200 in free-floating sections or paraffin embedded sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
osm-9-like TRP channel 2; OTRPC2
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Caterina, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10201375'>Nature 398, 436-41 (Apr 1, 1999). Jahnel, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=14622291'>Eur J Biochem 270, 4264-71 (Nov, 2003). Montell, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11864597'>Mol Cell 9, 229-31 (Feb, 2002).
Specificity:
Immunohistochemical analysis in rat dorsal root ganglia and spinal cord indicates a high level of specificity for this antiserum. Specificity was also shown by Western blot. This antibody is known to react with rat and human VRL-1.
Rabbit anti-Vanilloid receptor-like protein 1 (VRL-1) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen.
Background Info:
TISSUE SPECIFICITY: Ubiquitously expressed. Expressed in dorsal root ganglia, trigeminal ganglia, spinal chord (Lissauer's tract, dorsal horn and dorsal columns) (at protein level). PTM: N-glycosylated. PTM: Phosphorylated by PKA. SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4, containing 0.02% sodium azide as preservative.
Host Animal:
Rabbit
Species Reactivity:
Human,Rat
Immunogen:
A synthetic peptide (C-KNSASEEDHLPLQVLQSP) as part of rat VRL-1 protein (aa: 744-761) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC, Immunofluorescence, Western blot and Immunoblot. Recommended to be used at a concentration of 0.5-1 µg for these applications. This antiserum works superbly for immunohistochemistry on free-floating or paraffin embedded sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
osm-9-like TRP channel 2; OTRPC2
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Caterina, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10201375'>Nature 398, 436-41 (Apr 1, 1999). Jahnel, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=14622291'>Eur J Biochem 270, 4264-71 (Nov, 2003). Montell, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11864597'>Mol Cell 9, 229-31 (Feb, 2002).
Specificity:
Immunohistochemical analysis in rat dorsal root ganglia and spinal cord indicates a high level of specificity for this antiserum. Specificity was also shown by Western blot. This antibody is known to react with rat and human VRL-1.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer, and Parkinson disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst mammalian species.
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting: A concentration of 1-10 µg/mL is recommended for this application. In Western Blotting, this antibody detects multiple BDNF isoforms (14 kDa mature BDNF, 18 kDa isoform, 28 kDa BDNF dimer/truncated BDNF, 32 kDa proBDNF monomer) depending on sample application (human serum, cell lysate, tissue homogenate). IHC: Antibody works well in immunohistochemistry with the proper fixation, pretreatments and dilution. Formal fixed, paraffin embedded tissue is not recommend. Recommended fixation is Zamboni fixative or light 4% PFA fixation on fixed, frozen tissue. Recommended dilution is 1-10 µg/mL for immunohistochemistry at 4 degrees centigrade for 2-48 hours. ELISA: 1-10 µg/mL capture/detection. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A Acheson et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7700353'>(1995) Nat. 74: 450-3 Q Yan et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8083736>(1994) J. Neurosci. 14(9): 5281-91 XF Zhou et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8895863>(1996) Neurosci. 74: 945-53 XF Zhou, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9454633>(1998) Exp. Neurol. 149: 237-42 B Mellstrom et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15581398>(2004) Crit Rev Neurobiol 16, 43-9 I Tapia-Arancibia et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15571756>(2004) Front Neuroendocrinol 25, 77-107 S Pezet, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12589922> (2002) Brain Res Brain Res Rev 40, 240-9 Barde Y. A. et al (1989) EMBO J. 1: 549 Conner J et al. (1997) J. Neurosci. 17: 2295 JA Coull et al http://www.ncbi.nlm.nih.gov/pubmed/16355225> (2005) Nature. Dec 15;438(7070):1017-21. C Gomes et al http://www.ncbi.nlm.nih.gov/pubmed/23363775'> (2013) J Neuroinflammation. Jan 30;10:16.
Specificity:
Less than 0.1% cross reactivity with mouse NGF, recombinant human NT3 and NT4/5 has been recorded by dot blot analysis. This antiserum is known to recognise rat, human and human BDNF, and is expected to react with BDNF from other species due to amino acid sequence homology.
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Guinea Pig,Mouse,Pig,Rabbit,Rat
Immunogen:
A synthetic peptide (GLFSSYRLPGHTQDTLVAQKSS) as a part of porcine ChAT protein (aa: 167-188) conjugated to KLH
Applications:
ELISA,ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
Mixed
Application Details:
Immunohistochemistry/cytochemistry on 4% PFA or formalin fixed frozen sections; paraffin sections can be more difficult and require more extensive antigen recovery methods. 1:400-1:2000 depending up detection and incubation times ELISA: direct antigen ELISA This antiserum will superbly stain both cell bodies and nerve terminal, and works particularly well in enteric and peripheral neurons. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Benecke et al. (1993). J. Neurochemistry. 61: 804-811
Specificity:
This antiserum stains cholinergic neurons in guinea-pig and rabbit. This antiserum is known to react with ChAT of origin guinea-pig, mouse, rat and rabbit.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human BDNF
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA (1 site), Western Blot, immunoblot, inhibition of biological activity in vitro/in vivo. Recommended to be used at an amount of 1-10 µg/mL for immunohistochemistry, Western blot or immunoblot, 0.1 µg/mL for ELISA and for inhibition of biological activity in vitro 1-10 µg/mL. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum stains cell bodies and some nerve terminals in the dorsal horn of the rat spinal cord, however, does not stain finest nerve terminals. Western blot: 1-10 µg/mL. Tissue homogenate is a recommended sample application for Western Blotting. The antibody detects 14 kDa (mature BDNF), 32 kDa (proBDNF) and a 18 kDa BDNF isoform, however numerous other non-characterized bands may also be present. In cell lysates, only 18 kDa and 32 kDa BDNF are detected. Alternative antibodies for Western Blotting are: R-1707-100 (cell lysates and tissue homogenates), R-083-100/R-066-500 (cell lysates, tissue homogenates and human serum); M-1744-50/100 (human serum and tissue homogenates). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A Acheson et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7700353'>(1995) Nat. 74: 450-3 Q Yan et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8083736'>(1994) J. Neurosci. 14(9): 5281-91 XF Zhou et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8895863'>(1996) Neurosci. 74: 945-53 XF Zhou, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9454633'>(1998) Exp. Neurol. 149: 237-42 B Mellstrom et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15581398'>(2004) Crit Rev Neurobiol 16, 43-9 I Tapia-Arancibia et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15571756'>(2004) Front Neuroendocrinol 25, 77-107 S Pezet, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12589922'> (2002) Brain Res Brain Res Rev 40, 240-9 Barde Y. A. et al (1989) EMBO J. 1: 549 Conner J et al. (1997) J. Neurosci. 17: 2295 JA Coull et al http://www.ncbi.nlm.nih.gov/pubmed/16355225'> (2005) Nature. Dec 15;438(7070):1017-21. C Gomes et al http://www.ncbi.nlm.nih.gov/pubmed/23363775'> (2013) J Neuroinflammation. Jan 30;10:16.
Specificity:
Less than 0.1% cross-reactivity against NGF, recombinant NT3 and NT4 by dot blot. This antiserum is known to react with BDNF from rat, mouse and human. Expected to react with BDNF of other species due to amino acid sequence homology.
Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released. This antibody is raised in sheep to detect the prodomain of NGF not the mature peptide.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
The recombinant prodomain fragment of human nerve growth factor
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, Immunofluorescence, ELISA, Western Blot, biological neutralization of proNGF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
pro-brain nerve growth factor; proNGF; NGF;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Srinivasan B et al. J Biol Chem. 2004 Oct 1;279(40):41839-4 Yune TY et al. J Neurosci. 2007 Jul 18;27(29):7751-61.
Specificity:
The specificity of this antibody has been confirmed by WB. It does NOT crossreact with proBDNF, proNT-3 or mature NGF. Confirmed to react with purified human proNGF and crossreact with mouse and rat proNGF
Brain derived neurotrophic factor (BDNF) is synthesized as a precursor (proBDNF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proBDNF is synthesized in neurons and glia (eg., microglia), transported anterogradely and retrogradely and may be released in an activity dependent manner. This antibody is raised in sheep to detect the prodomain of BDNF and not the mature peptide.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
The recombinant prodomain fragment of human brain-derived neurotrophic factor
Applications:
ELISA,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
ELISA, Western Blot, biological neutralization of proBDNF, Immunocytochemistry/Immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Proform brain derived neurotrophic factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Fan, YJ. et al http://www.ncbi.nlm.nih.gov/pubmed/18430030?ordinalpos=6&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum'> (2008) Eur J Neurosci 27(9):2380-90. Ulman, L. et al http://www.ncbi.nlm.nih.gov/pubmed/18971468'> (2008) J Neurosci. Oct 29;28(44):11263-8.
Specificity:
Confirmed to react with purified human proBDNF, crossreact with mouse and rat proBDNF Cross reactivity with other species than human, mouse and rat has not yet been tested
FUNCTION: Seems to promotes the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Chicken,Human,Rat
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human, mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Zouh et al. (1996) Mol. Neurobio. 13(3): 185-97 Rush et al. (1997) Clin. Exp. Pharmacol. 24: 549-55 Tafreshi et al. (1998) Neurosci. 83(2): 373-80
Specificity:
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. This antiserum is known to react with rat, chicken and human NT3.
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Primate,Rat
Immunogen:
Recombinant human NT4
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA (1 site), Western Blot, inhibition of biological activity in vitro/in vivo. Recommended to be used at a concentration of 2-10 µg/mL for immunohistochemistry, ELISA and Western blot and inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). Note that the concentration of NT4 is generally low in most tissues nevertheless, neonatal testes of rat can be used as a good positive control. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Lodouichi (2000) J. Neuroscie. 20(6): 2155 Zhang et al. (1999) J Neurosci. Meths. 89 (1): 69
Specificity:
Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by dot blot. Known to react with NT4 from rat and human, mouse and monkey.
FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Primate,Rat
Immunogen:
Recombinant human NT4
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA (1 site), Western Blot, dot blot, inhibition of biological activity in vitro/in vivo. Recommended to be used at a dilution of 1:500 to 1:2000 for immunohistochemistry, ELISA and Western blot. 1:10 to 1:50 for inhibition of biological activity in vitro. Use neat for in vivo studies at 5-10 µL/g body weight. Note that the concentration of NT4 is generally low in most tissues nevertheless, neonatal testes of rat can be used as a good positive control. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Lodouichi (2000) J. Neuroscie. 20(6): 2155 Zhang et al. (1999) J Neurosci. Meths. 89 (1): 69
Specificity:
Less than 1% cross-reactivity against NGF, recombinant human BDNF and 5% to NT3 has been shown by 1-site ELISA. Known to react with NT4 from rat and human, mouse and monkey.
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display sevvere movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Chicken,Human,Rat
Immunogen:
Recombinant human NT3
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. This antiserum will neutralise NT3 but not other neurotrophins. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Zouh et al. (1996) Mol. Neurobio. 13(3): 185-97 Rush et al. (1997) Clin. Exp. Pharmacol. 24: 549-55 Tafreshi et al. (1998) Neurosci. 83(2): 373-80
Specificity:
A cross reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by 1-site ELISA. This antiserum is known to react with rat, chicken and human NT3.
The protein named TrkB (also named Neurotrophic tyrosine kinase receptor type 2 (NTRK2), GP145-TrkB or Tropomyosin-related kinase B is a receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems and is important in the regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. TrkB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. TrkB is the primary receptor for BDNF (brain-derived neurotrophic factor. TrkB also binds NT4 and NT3 but less efficiently. Upon ligand-binding, the receptor undergoes homodimerization, autophosphorylation and activation. TrkB activation recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that each regulate distinct overlapping signaling cascades within cells. Through SHC1, FRS2, SH2B1, SH2B2, these activate the GRB2-Ras-MAPK cascade that regulates, for instance, neuronal differentiation including neurite outgrowth. These same effectors also control the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. TrkB, via activation of PLCG1 and the downstream protein kinase C-regulated pathways, also controls synaptic plasticity, and thus plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. One such consequence is that PLCG1 activation via TrkB is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. (Reference: www.uniprot.org)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, PBS, pH 7.2-7.6, 0.1% trehalose, without preservatives.
Western Blotting (0.5 - 2 µg/mL). Cell lysates or membrane preparations prepared from isolated brain or spinal cord tissues are recommended. This antibody works on skim milk blocked membranes, however, best results are obtained with a equal mixture of 2.5% skim milk / 2.5% highly purified BSA as blocking reagent and antibody diluent. Immunohistochemistry (1 - 5 µg/mL). Antibody has been shown to work on PFA fixed, frozen sections. TBS is preferred for buffer preparation. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Detects full-length TrkB phosphorylated at amino acid S479 in humans (S478 in mouse and rat).
Positive Control:
Retinoic acid- and BDNF-treated NSC34 cells
References:
Lai K-O. et al. (2012), Nature Neuroscience 15, 1506-1515, doi:10.1038/nn.3237. Zhao L. et al. (2009), Journal of Cell Science 122, 3123-3136, doi: 10.1242/jcs.047712.
Specificity:
Human TrkB (pS478). Antibody has been shown to be specific for TrkB phosphorylated on serine 478 by phospho-peptide absorption dot blots, and on cell lysates from cell lines induced with retinoic acid and BDNF. Antibody detects a clear band in retinoic acid (RA) and BDNF-treated NSC34 cell lysates at ~120 kDa only, demonstrating that the phosphorylated TrkB receptor is being detected. Additional non-specific bands at lower molecular weight may be observed in lysates and homogenates with the antibody and these bands have not been characterized.
Product Validation Info:
Antibody is specific for TrkB serine phosphorylation at amino acid 478/479 (rodents/human). Lambda-phosphatase treatment obliterates positive staining.
The protein named TrkB (also named Neurotrophic tyrosine kinase receptor type 2 (NTRK2), GP145-TrkB or Tropomyosin-related kinase B is a receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems and is important in the regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. TrkB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. TrkB is the primary receptor for BDNF (brain-derived neurotrophic factor. TrkB also binds NT4 and NT3 but less efficiently. Upon ligand-binding, the receptor undergoes homodimerization, autophosphorylation and activation. TrkB activation recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that each regulate distinct overlapping signaling cascades within cells. Through SHC1, FRS2, SH2B1, SH2B2, these activate the GRB2-Ras-MAPK cascade that regulates, for instance, neuronal differentiation including neurite outgrowth. These same effectors also control the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. TrkB, via activation of PLCG1 and the downstream protein kinase C-regulated pathways, also controls synaptic plasticity, and thus plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. One such consequence is that PLCG1 activation via TrkB is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. (Reference: www.uniprot.org)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, PBS, pH 7.2-7.6, 0.1% trehalose, without preservatives.
Western Blotting (0.5 2 µg/mL). High skim milk concentration (5%) causes suppression of pY817 signal, suggesting that 5% skim milk should be avoided as blocking buffer and antibody diluent. Strongest signal is obtained in 5% BSA blocking buffer, however, many non-specific bands are present. An equal mixture of skim milk and BSA (2.5% each) appears to provide the best compromise between signal and noise. However, optimization of blocking condition is recommended for each particular sample, with excess of BSA over skim milk likely to be beneficial for best results. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Detects full-length TrkB phosphorylated at amino acid Y817 in humans (Y816 in mouse and rat).
Positive Control:
Retinoic acid- and BDNF-treated NSC34 cells
Specificity:
Human TrkB (pY817). Antibody has been shown to be specific for TrkB phosphorylated on tyrosine 817 by phospho-peptide absorption dot blots, and on cell lysates from cell lines induced with retinoic acid and BDNF. Antibody detects a clear band in retinoic acid (RA) and BDNF-treated NSC34 cell lysates at ~120 kDa only, demonstrating that the phosphorylated TrkB receptor is being detected. While not fully tested, this antibody may detect phosphorylated TrkA (pY791/794, human/rodent) and TrkC (pY834/820/859, human/mouse/rat) due to high degree of amino acid homology surrounding the phosphorylation site.
Product Validation Info:
Antibody detects a clear band in retinoic acid (RA) and BDNF-treated NSC34 cell lysates at ~120 kDa only
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.05% sodium azide.
Host Animal:
Rabbit
Species Reactivity:
Human,Other Mammals (Predicted)
Immunogen:
Antibody was raised against a GST-tagged rhBDNF fusion protein and expressed in and purified from E. coli.
Applications:
ELISA
Antibody Isotype:
IgG
Application Details:
Western Blotting (denaturing and reducing): 0.2 to 1 µg/mL. Antibody detects 14 kDa mature BDNF monomer and 32 kDa proBDNF monomer in cell lysate and tissue homonenate. Antibody has only been tested on cell lysate and tissue homogenate of human origin. Acid-treated samples may give cleaner blots, and enhance signals for BDNF. R-1707-100 is not recommended for human serum samples. For human serum analysis, we recommend mouse monoclonal antibody to rhBDNF (M-1744-50/100), or rabbit polyclonal antibody to BDNF peptide 1-10 (R-083-100, whole serum; R-066-500, IgG). Flow Cytometry: ~2 µg per 10^6 cells, methanol fixation. Note: R-1707-100 cannot be used to distinguish the flow cytometry signal originating from mature BDNF versus proBDNF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Brain-derived neurotrophic factor; Abrineurin
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human, rat and mouse BDNF. Expected to detect BDNF from other species due to sequence homology. No cross-reactivity with other neurotrophins.
Rabbit anti-Tyrosine Hydroxylase (TH) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. FUNCTION: Plays an important role in the physiology of adrenergic neurons. CATALYTIC ACTIVITY: L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin. COFACTOR: Fe(2+) ion. ENZYME REGULATION: Phosphorylation leads to an increase in the catalytic activity. PATHWAY: Catecholamine biosynthesis; first step. SUBUNIT: Homotetramer. PTM: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation. SIMILARITY: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Guinea Pig,Human,Mouse,Rat
Immunogen:
A synthetic peptide (PRFIGRRQSLIEDARK) as part of human Tyrosine Hydroxylase (63-78) conjugated to KLH has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from TH protein in rat (31-47).
Applications:
IHC-Frozen
Antibody Isotype:
IgG
Application Details:
IHC. A concentration of 4-10 µg/mL is recommended for this application. This is a superb antibody for detection of tyrosine hydroxylase containing neurons exhibiting an intense labelling with a negligible background. This antiserum has proven extremely useful for staining of catecholaminergic neurons. It stains nicely and intensely dendritic processes and fine nerve terminals. We recommend mouse or rat brain containing catecholaminergic neurons as a positive control for this antibody, for example brain stem or striatum. Western blotting: A concentration of 5-15 µg/mL is recommended.Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Mallett, J. Trends in Pharmacological Science. 17(4): 129-135, 1996. Haavik, J. et al. Mol. Neurobiology 16(3) :285-309, 199 Lewis DA, et al, Neuroscience 54: 477, 1993 Kumer S.C. et al. Journal of Neurochemistry, 67(2) :443-462, 199 Haycock, J. Anal. Biochemistry 181: 259-266, 198 Haycock, J. Anal. Biochemistry 208: 397-399, 199 Renfroe, J.B., et al. Brain Res. Bull. 13: 109-126, 198 Xu, Z et a.l Neurosci. 82(3): 727, 1998
Specificity:
IHC on brain shows a pattern of staining specific for TH containing neurons. This antibody is known to react with rat, mouse and guinea pig. Cross reactivity with other species has not yet been tested.
Rabbit anti-Leptin Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Leptin is secreted by white adipocytes and functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. Leptin has a variety of other roles including endocrine functions, regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing (Ref Entrez Gene).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
Recombinant rat Leptin protein
Applications:
ELISA,WB
Antibody Isotype:
Mixed
Application Details:
WB and ELISA. Suggested dilution of 1:1,000-1:4,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Rabbit anti-Leptin Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Leptin is secreted by white adipocytes and functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. Leptin has a variety of other roles including endocrine functions, regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing (Ref Entrez Gene).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant human Leptin protein
Applications:
ELISA,WB
Antibody Isotype:
Mixed
Application Details:
WB and ELISA. Suggested dilution of 1:1,000-1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Rabbit anti-Tyrosine Kinase Receptor A (TrkA) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, ELISA.
Background Info:
TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Cell membrane; single-pass type I membrane protein. Endocytosed to the endosomes upon treatment of cells with NGF. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. Both isoforms have similar biological properties. TISSUE SPECIFICITY: Isoform TrkA-II is primarily expressed in neuronal cells. Isoform TrkA-I is found in non-neuronal tissues. Mutations in TrkA have been associated with congenital insensitivity to pain, anhidrosis, self-mutalating behaviour, mental retardation and cancer.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Extracellular domain of glycosylated human TrkA protein produced in mammalian cells was used as the immunogen.
Applications:
ELISA,IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC (1:1000 dilution), Western Blotting (denaturing and non-reducing SDS-PAGE, 1:1000-1:2500 dilution), 1-site ELISA (1:5000 dilution). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Zhang FX, et al. (2005) Brain Res 16; 1062 (1-2) pp. 92-100. Gearhart DA, et al. (2006) J Neurosci Methods. 150(2) pp. 159-173.
Specificity:
Specificity was demonstrated by immunohistochemistry. When used for immunohistochemistry in rat dorsal root ganglia, staining is restricted to the known distribution of TrkA, that is in small, nociceptive neurons. Reacts with human, rat and mouse TrkA. Other species not yet tested.
Rabbit anti-Tyrosine Kinase Receptor C (TrkC) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
TrkC is a member of the neurotrophic tyrosine receptor kinase family. TrkC is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC is the receptor for neurotrophin-3 (NT-3). Signalling through TrkC leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 4 named isoforms produced by alternative splicing. Additional isoforms seem to exist. Mutations in TrkC have been associated with medulloblastomas, secretory breast carcinomas and other cancers.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Extracellular domain of glycosylated human TrkC protein produced in CHO cells was used as the immunogen.
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1:3000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Zhang FX, et al. (2005) Brain Res. 1062 (1-2) pp. 92-100.
Specificity:
Specificity was demonstrated by immunohistochemistry. This antibody was used to stain cryostat sections of the rat peripheral sensory ganglia. Reacts with human, rat and mouse TrkC. Other species have not yet been tested.
Rabbit anti-Somatostatin Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Somatostatin is secreted by neuroendocrine neurons in hypothalamus and interacts with somatostatin receptors inhibiting secretion of somatotropin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with BSA
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic Somatostatin peptide (103-116) conjugated to BSA.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded
Antibody Isotype:
IgG
Application Details:
A dilution of 5-10 µg/mL is recommended for immunohistochemistry using formalin fixed and paraffin embedded tissues and for 4% paraformaldehyde fixed frozen tissues. A dilution of 5-15 µg/mL is recommended for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rabbit anti-Tyrosine Kinase Receptor B (TrkB) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, ELISA.
Background Info:
TrkB is a member of the neurotrophic tyrosine receptor kinase family. It is a membrane-bound receptor and upon neurotrophin binding, it phosphorylates itself as well as MAPK pathways members. TrkB is the receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). It is Involved in the development and/or maintenance of the nervous system. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 4 named isoforms produced by alternative splicing. Additional isoforms seem to exist. TISSUE SPECIFICITY: The different forms are differentially expressed in various cell types. SIMILARITY: Belongs to the Tyr protein kinase family. Insulin receptor subfamily. SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SIMILARITY: Contains 2 LRR (leucine-rich) repeats. SIMILARITY: Contains 1 protein kinase domain. Mutations in the TrkB gene have been associated with obesity and mood disorders.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
Extracellular domain of glycosylated mouse TrkB protein produced in CHO cells was used as the immunogen. As shown for similar antisera, it is anticipated that this antibody will block the TrkB receptor binding of corresponding neurotrophin ligand.
Applications:
ELISA,IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IF (1:1000-1:3000), 1-site ELISA (1:10,000 dilution). A dilution of 1:1000 to 1:3000 o/n is recommended for IF. 4% PFA frozen sections tested. Not yet tested on paraffin embedded tissues. Use triton X-100 permeabilizaiton with frozen sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Desmet CJ, Peeper DS (2006) Cell Mol Life Sci. 63(7-8) pp. 755-9 Mizoguchi Y et al., J Immunol. 2009 Dec 15;183(12):7778-8 Spencer-Segal JL et al. J Neurosci. 2011 May 4;31(18):6780-90. Nakajima K et al. Glia. 1998 Nov;24(3):272-8
Specificity:
Specificity was demonstrated by immunohistochemistry. This antibody was used to stain cryostat sections of the rat peripheral sensory ganglia. Reacts with rat and mouse TrkB. Other species have not yet been tested.
Rabbit anti-Neuron specific enolase (NSE) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Enolase is a metalloenzyme that catayzes the reaction between 2-phospho-D-glycerate and phosphoenolpyruvate during glycolysis. Mammalian enolase is composed of 3 subunits; alpha, beta and gamma (Neuron-specific enolase). These subunits can form homodimers or heterodimers. The alpha/gamma heterodimer and the gamma/gamma homodimer are found primarily in neurons.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human Neuron Specific Enolase (NSE) expressed in and purified from E.coli
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:1,000 - 1:2,000 is recommended for WB. A dilution of 1:500 is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rabbit anti-Myristoylated alanine rich C kinase substrate (MARCKS) Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Myristoylated alanine-rich C-kinase substrate (MARCKS) is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. Ref: SWISSPROT.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant full length MARCKS expressed and purified from E. coli
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunocytochemistry (IC). A dilution of 1:10,000 - 20,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Myristoylated alanine-rich C-kinase substrate; MARCKS; Protein kinase C substrate 80 kDa protein;Marcks; Macs;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB. Does not react with rodent protein.
Purification:
Whole serum
Target:
Myristoylated alanine rich C kinase substrate (MARCKS)
Rabbit anti-Glial Fibrillary Acidic Protein (GFAP) Polyclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
Background Info:
GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Bovine,Horse,Human,Mouse,Pig,Rat
Immunogen:
Recombinant full length human GFAP isotype 1 expressed in and purified from E. coli.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:5,000 is recommended for WB, and 1:1,000 - 1:5,000 for ICC and IHC. A dilution of 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme-linked methods is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Astrocyte; Glial fibrillary acidic protein; GFAP;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Reeves S.A, et al. Proc. Natl. Acad. Sci. U.S.A. 86:5178-5182(1989). Brenner M, et al. Brain Res. Mol. Brain Res. 7:277-286(1990). Isaacs A, et al. Genomics 51:152-154(1998). Ota T, et al. Nat. Genet. 36:40-45(2004). Nielsen A.L, et al. J. Biol. Chem. 277:29983-29991(2002). Singh R, et al. Genomics 82:185-193(2003). Brenner M, et al. Nat. Genet. 27:117-120(2001). Brockmann K, et al. Eur. Neurol. 50:100-105(2003). Stumpf E, et al. Arch. Neurol. 60:1307-1312(2003). Sawaishi Y, et al. Neurology 58:1541-1543(2002). Aoki Y, et al. Neurosci. Lett. 312:71-74(2001).
Rabbit anti-Tyrosine Kinase Receptor B (TrkB) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
FUNCTION: Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures (By similarity). Binds APS. Interacts with SQSTM1. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. Additional isoforms seem to exist. TISSUE SPECIFICITY: The different forms are differentially expressed in various cell types. Isoform T2 is primarily expressed in neurons. PTM: Ligand-mediated auto-phosphorylation. SIMILARITY: Belongs to the Tyr protein kinase family. Insulin receptor subfamily. SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SIMILARITY: Contains 2 LRR (leucine-rich) repeats. SIMILARITY: Contains 1 protein kinase domain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide (AFPRLEPNSIDPEN(C)) as part of rat TrkB protein (aa: 54-67) conjugated to KLH
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1:3000 is recommended for this application. For WB, a 1:500 to 1:1000 dilution is recommended. The sequence of the immunogen and the conjugate are identical to the one used by Yan et al (see the Refs) and the antiserum appears to have similar characteristics. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Yan Q et al. J Comp Neurol 378:135-57 (1997). Stoilov, P., et al., Biochem. Biophys. Res. Commun. 290(3):1054-1065 (2002). Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Nakagawara, A., et al., Genomics 25(2):538-546 (1995). Haniu, M., et al., Arch. Biochem. Biophys. 322(1):256-264 (1995). Allen, S.J., et al., Neuroscience 60(3):825-834 (1994). Mizoguchi Y et al., J Immunol. 2009 Dec 15;183(12):7778-8 Spencer-Segal JL et al. J Neurosci. 2011 May 4;31(18):6780-90. Nakajima K et al. Glia. 1998 Nov;24(3):272-8
Specificity:
Sequence was chosen to avoid cross reactivity with TrkA and TrkC. This antiserum recognises human, rat and mouse TrkB. Cross reactivity with other species has not yet been tested.
Rabbit anti-Tyrosine Hydroxylase (TH) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. FUNCTION: Plays an important role in the physiology of adrenergic neurons. CATALYTIC ACTIVITY: L-tyrosine + tetrahydrobiopterin + O2 = 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin. COFACTOR: Fe(2+) ion. ENZYME REGULATION: Phosphorylation leads to an increase in the catalytic activity. PATHWAY: Catecholamine biosynthesis; first step. SUBUNIT: Homotetramer. PTM: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation. SIMILARITY: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. The presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Guinea Pig,Mouse,Rat
Immunogen:
A synthetic peptide (PRFIGRRQSLIEDARK) as part of human Tyrosine Hydroxylase (63-78) conjugated to KLH has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from TH protein in rat (31-47).
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
Immunohistochemistry (IHC): 1:2,000 to 1:5,000, dilutions of up to 1:100,000 have been reported. This is a superb antibody for detection of tyrosine hydroxylase (TH) containing neurons exhibiting an intense labelling with a negligible background. This antiserum has proven extremely useful for staining of catecholaminergic neurons. It stains nicely and intensely dendritic processes and fine nerve terminals. We recommend mouse or rat brain containing catecholaminergic neurons as a positive control for this antibody, for example brain stem or striatum. Western blotting (WB) : 1:100 to 1:500. Antibody has been tested on RIPA-extracted PC12 cell lysate and shown to be specific for TH (~60 kDa). Tissue homogenates show a higher level of non-specific binding and presence of uncharacterized bands. Affinity-purified anti-TH antibody (R-148-50) is recommended for tissue homogenates. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Mallett, J. Trends in Pharmacological Science. 17(4): 129-135, 1996. Haavik, J. et al. Mol. Neurobiology 16(3) :285-309, 199 Lewis DA, et al, Neuroscience 54: 477, 1993 Kumer S.C. et al. Journal of Neurochemistry, 67(2) :443-462, 199 Haycock, J. Anal. Biochemistry 181: 259-266, 198 Haycock, J. Anal. Biochemistry 208: 397-399, 199 Renfroe, J.B., et al. Brain Res. Bull. 13: 109-126, 198 Xu, Z et a.l Neurosci. 82(3): 727, 1998
Specificity:
IHC on brain shows a pattern of staining specific for TH containing neurons. This antibody is known to react with rat, mouse and guinea pig. Cross reactivity with other species has not yet been tested.
Mouse anti-Tyrosine Kinase Receptor C (TrkC) Monoclonal Antibody (Unconjugated), suitable for FC, ICC.
Background Info:
Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation (Ref: uniprot.org).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS, pH 7.2-7.6 with 0.1% trehalose and contains no preservatives.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic peptide immunogen, KEPFPESTDNFI, cooresponding to amino acids 397-408 of human TrkC ECD.
Applications:
FC,ICC
Clone number:
BS337
Antibody Isotype:
IgG2b, kappa
Application Details:
Flow Cytometry (5-10 µg/mL): Tested on human and rodent cell lines. Cell staining can be performed under native conditions on ice, or on fixed cells with up to 4% formaldehyde. Immunocytochemistry (1-2 µg/mL): Tested on fixed (4% formaldehyde) human cells. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Mouse anti-Tyrosine Kinase Receptor B (TrkB) Monoclonal Antibody (Unconjugated), suitable for FC.
Background Info:
The protein named TrkB (also named Neurotrophic tyrosine kinase receptor type 2 (NTRK2), GP145-TrkB or Tropomyosin-related kinase B is a receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems and is important in the regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. TrkB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. TrkB is the primary receptor for BDNF (brain-derived neurotrophic factor. TrkB also binds NT4 and NT3 but less efficiently. Upon ligand-binding, the receptor undergoes homodimerization, autophosphorylation and activation. TrkB activation recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that each regulate distinct overlapping signaling cascades within cells. Through SHC1, FRS2, SH2B1, SH2B2, these activate the GRB2-Ras-MAPK cascade that regulates, for instance, neuronal differentiation including neurite outgrowth. These same effectors also control the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. TrkB, via activation of PLCG1 and the downstream protein kinase C-regulated pathways, also controls synaptic plasticity, and thus plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. One such consequence is that PLCG1 activation via TrkB is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. (Reference: www.uniprot.org)
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS, pH 7.2-7.6 with 0.1% trehalose.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic peptide immunogen, ESSKNIPLANLQ. Extracellular domain of TrkB (amino acids 179-190 of human TrkB).
Applications:
FC
Clone number:
BS379
Antibody Isotype:
IgG2b, kappa
Application Details:
Flow Cytometry (5-10 µg/mL): Tested on human and rodent cell lines. Cell staining can be performed under native conditions on ice, or on fixed cells with up to 4% formaldehyde. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
GFAP isolated biochemically from pig spinal cord was used as the immunogen.
Applications:
IHC-Frozen,WB
Clone number:
2A5
Antibody Isotype:
IgG1
Application Details:
Western Blot (1:1,000-1:2,000): tested on rat, mouse brain and spinal cord, human recombinant protein, pig brain. Immunohistochemistry (1:500-1:1,000): tested on rat cerebellum section. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Glial fibrillary acidic protein; GFAP
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody is specific for GFAP as demonstrated by western blotting and immunohistochemistry.
Mouse anti-RNA-binding protein Musashi homolog 1 Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC, ELISA.
Background Info:
Musashi 1 is an RNA binding protein that regulates the expression of target mRNAs at the translational level. It contains two conserved tandem RNA recognition motifs. Musashi 1 binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. It may play a role in the proliferation and maintenance of stem cells in the central nervous system. It is detected in fetal kidney, brain, liver and lung and adult brain and pancreas. It is also detected in hepatoma cell lines.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.4.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Full length recombinant human Musashi 1 protein (1-87) with a GST tag.
Applications:
ELISA,ICC,IHC-Frozen,WB
Clone number:
3F2
Antibody Isotype:
IgG2a
Application Details:
This antibody is recommended for WB, IHC, sandwich ELISA and immunofluorescence. The recommended dilution for this antibody is 3 µg/mL for IHC and 10 µg/mL for immunofluorescence. The antibody has been used successfully on formalin fixed, paraffin embedded tissue. It is necessary to treat the paraffin sections by placing them in 1X Citrate Buffer (pH 6.0) then placing in a Pressure Cooker at 125ºC for 4 minutes then at 90ºC for 45 min. The tissue may also be treated by placing it in 1X Citrate Buffer (pH 6.0) then microwaving at 750W for 20 minutes. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
RNA-binding protein Musashi homolog 1; Musashi1; MSI1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen.
Mouse anti-Tyrosine Kinase Receptor A (TrkA) Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
TrkA is a member of the neurotrophic tyrosine kinase receptor family. It is a membrane-bound receptor that upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). TrkA leads to cell differentiations and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Cell membrane; single-pass type I membrane protein. Endocytosed to the endosomes upon treatment of cells with NGF. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. Both isoforms have similar biological properties. TISSUE SPECIFICITY: Isoform TrkA-II is primarily expressed in neuronal cells. Isoform TrkA-I is found in non-neuronal tissues. Mutations in TrkA have been associated with congenital insensitivity to pain, anhidrosis, self-mutalating behaviour, mental retardation and cancer (Reference: www.uniprot.com).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS, pH 7.4, containing 3% trehalose without preservatives.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide from the intracellular cytoplasmic domain of human TrkA (C-HGPDAKLLAGGE, aa: 604-615) has been used as the immunogen.
Applications:
ICC,WB
Clone number:
BS292
Antibody Isotype:
IgG3, kappa
Application Details:
WB: Western blotting: 1-3 µg/mL, antibody is suitable for reduced (with DTT or beta-mercaptoethanol) and non-reduced samples. Denatured but not reduced samples provides cleaner blot signals as demonstrated in our photographs. Immunocytochemistry: 1-2 µg/mL. Antibody works on 4% formaldehyde-fixed cells. Note that cells require a permeabilization step, because the antibody detects a cytoplasmic epitope of TrkA. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Cabrera N. et al., TrkA receptor ectodomain cleavage generates a tyrosine-phosphorylated cell-associated frangment. J Cell Biol, 1996, Vol.132(3), pp.427-436. Rossi F.M. et al., Expression of the nerve growth factor receptors TrkA and p75NTR in the visual cortex of the rat: development and regulation by the cholinergic input. J Neurosci, Februar 1, 2002, 22(3):912-919.
Specificity:
TrkA. Does not cross-react with TrkB or TrkC. Reacts with human TrkA. Known to cross-react with TrkA from rat and mouse. Expected to cross-react with other mammalian species based on peptide antigen sequence similarity.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Goat
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB and direct ELISA. A dilution range of 1:1000 - 1:5000 is recommended for IHC and WB. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor; Beta-NGF; NGF; NGFB;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Ebendal, T. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2468788'> (1989) J Neurosci Res 22, 223-240. Zhou, X. F. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation_uids=7815824'> (1994) J Neurosci Methods 54, 95-102. Angeletti, P. U. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=4880217'> (1968) Adv Enzymol Relat Areas Mol Biol 31, 51-75. Hesse K. et al. http://www.ncbi.nlm.nih.gov/pubmed/9291146'> (1997) Neurosci Lett. Aug 8;231(2):83-6. 5. Miao J et al. http://www.ncbi.nlm.nih.gov/pubmed/23000062'> (2012) Neurosci Res. Dec;74(3-4):269-76.
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF. Other species have not yet been tested.
Chicken anti-Nestin Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity). Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from a solution containing PBS buffer pH 7.2-7.6, without preservatives.
Host Animal:
Chicken
Species Reactivity:
Human,Rat
Immunogen:
Part of recombinant human protein (amino acids 315-630).
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:2,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NES;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human,reacts with human and rat. Other species not tested.
Transforming growth factor beta-1 (TGFB1) is a multi-functional cytokine with roles in proliferation, differentiation and other functions in many cell types. The secreted TGFB1 protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide (Ref: SWISS-Prot).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Pig,Rat
Immunogen:
A peptide from human and mouse Transforming growth factor beta-1 (372-386 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
TGFB1; TGFB; TGF-beta-1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
TGF beta 1, cross reactivity to other species is expected
Fatty acid-binding protein, adipocyte (ALBP) is a lipid transport protein which binds long chain fatty acids and other hydrophobic ligands and delivers them to their receptors in the nucleus. ALBP is found in the cytoplasm and nucleus of adipocytes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two human Fatty acid-binding protein, adipocyte peptide peptides (71-82 aa, 97-111 aa).
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
WB and ELISA. Suggested dilution of 1:1,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Fatty acid-binding protein, adipocyte (ALBP) is a lipid transport protein which binds long chain fatty acids and other hydrophobic ligands and delivers them to their receptors in the nucleus. ALBP is found in the cytoplasm and nucleus of adipocytes.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Human Fatty acid-binding protein, adipocyte peptide (97-111 aa).
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Adiponectin Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Adiponectin is synthesized by adipocytes and is involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities (ref: SWISSPROT).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide from human Adiponectin (230-244 aa).
Applications:
WB
Antibody Isotype:
IgY
Application Details:
WB. Suggested dilution of 1:2,000-1:5,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Rabbit anti-Microtubule-associated protein Tau (MAPT) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
FUNCTION: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. SUBCELLULAR LOCATION: Cytoplasm; cytosol. Cell membrane. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components. ALTERNATIVE PRODUCTS: 8 named isoforms produced by alternative splicing. Additional isoforms seem to exist. Isoforms differ from each other by the presence or absence of up to 5 of the 15 exons. One of these optional exons contains the additional tau/MAP repeat. TISSUE SPECIFICITY: Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system. DEVELOPMENTAL STAGE: Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain. DOMAIN: The tau/MAP repeat binds to tubulin. In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments and straight filaments, mainly composed of hyperphosphorylated forms of Microtubule-associated protein Tau. Defects in Microtubule-associated protein Tau are a cause of frontotemporal dementia and parkinsonism linked to chromosome 17, as well as a number of other neurodegenerative diseases.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Pig
Immunogen:
Recombinant human Microtubule-associated protein Tau, purified from E.coli.
Applications:
ELISA,WB
Antibody Isotype:
Mixed
Application Details:
WB and direct ELISA (Human). For WB a dilution of 1:500 is recommended. This antibody has been shown to detect the purified recombinant Tau expressed in E.Coli as well as a number of Tau isoforms in porcine cytosol. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; MAPT; MTBT1; TAU
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Goedert M, et al. (1988) Proc. Natl. Acad. Sci. U.S.A. 85:4051-4055. Goedert M, et al. (1989) Neuron 3:519-526. Lee G, et al. (1989) Neuron 2:1615-1624. Andreadis A, et al. (1992) Biochemistry 31:10626-10633. Jakes R, et al. (1991) EMBO J. 10:2725-2729. Cripps D, et al. (2006) J. Biol. Chem. 281:10825-10838. Maas T, et al. (2000) J. Biol. Chem. 275:15733-15740. Nacharaju P, et al. (1997) J. Neurochem. 69:1709-1719. Wintjens R, et al. (2001) J. Biol. Chem. 276:25150-25156. Oliva R, et al. (2004) Ann. Neurol. 55:448-44
Specificity:
Specificity was demonstrated by western blot. 20ng recombinant Tau is easily detected with smaller fragments likely representing degradation products from the purified protein, which has been expressed in E. Coli. This antiserum is known to react with human and pig Microtubule-associated Tau proteins. Other species have not yet been tested.
Rabbit anti-Nestin Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity). Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
Part of recombinant human protein (amino acids 315-630).
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human,reacts with Human. Other species not tested.
Chicken anti-Leptin Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Leptin is secreted by white adipocytes and functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. Leptin also has several endocrine functions and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing (Ref: Entrez Gene).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human
Immunogen:
Recombinant human Leptin protein
Applications:
ELISA,WB
Antibody Isotype:
IgY
Application Details:
ELISA and WB. Suggested dilution of 1:1,000-1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Chicken anti-Microtubule-associated protein 2 (MAP2) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ~280 kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with 5% trehalose
Host Animal:
Chicken
Species Reactivity:
Bovine,Human,Mouse,Rat
Immunogen:
Bovine MAP2 isolated from brain by the GTP microtubule cycling method.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). Suggested dilutions are: 1:10,00-1:20,000 (WB), 1:5,000-1:10,000 (ICC, IHC). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Microtubule-associated protein 2; MAP-2; MAP2;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by IC. Hu, Rat, Ms, Bov
Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Alpha-internexin is a ~66 kDa Class IV intermediate filament subunit expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with 5% trehalose
Host Animal:
Chicken
Species Reactivity:
Cat,Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant rat alpha-internexin expressed and purified from E. coli
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:5,000 - 10,000 is recommended for WB. A dilution of 1:500-1,000 is recommended for ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB. This antibody is specific for the 64-66 kDa alpha-internexin protein. Molecular weight will depend on species. Human, Rat, Mouse, Feline. It is predicted to react with other mammals.
Chicken anti-Glial fibrillary acidic protein (GFAP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded, IHC-Frozen, ICC.
Background Info:
Glial fibrillary acidic protein (GFAP) is approx. 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with 5% trehalose. The IgY preparation is at a concentration of ~25 mg/mL total protein.
Host Animal:
Chicken
Species Reactivity:
Cat,Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant GFAP (expressed in E.coli) and native bovine GFAP
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). WB: A dilution of 1:5,000 is recommended. ICC: A dilution of 1:1,000-1:5,000 using fluorescent secondary antibodies or peroxidase or other enzyme-linked methods is recommended on 4% PFA fixed cells in culture, with 3hr-o/n incubation of primary antibody. IHC: 4% PFA frozen tissues, permeabilized. IHC (paraffin-embedded): capable, HEIR treatment typically necessary. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Astrocyte; Glial fibrillary acidic protein; GFAP;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Brenner M. et al (2001) Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/11138011?"> Nat Genet. 2001 Jan;27(1):117-20.
Specificity:
The specificity of this antibody has been confirmed by WB. Human, Rat, Mouse, Feline. Predicted to react with other mammals.
Mouse anti-14-3-3 protein eta Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
14.3.3 protein eta or 14.3.3 binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (Ref SwissProt). 14.3.3 protein eta is widely expressed as both homodimers and heterodimers and are concentrated in the nervous system. High concentrations of 14.3.3 protein eta have been linked to Creutzfeld Jacob Disease, Parkinson's Disease and early-onset schizopherenia.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized with 5% trehalose
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Full length recombinant 14.3.3 protein ETA expressed in and purified from E. coli.
Applications:
ICC,IHC-Frozen,WB
Clone number:
3G12
Antibody Isotype:
IgG
Application Details:
WB, ICC, IHC. Suggested dilution of 1:500-1:1,000 for IHC and ICC. Suggested dilution of 1:1,000-1:5,000 for WB. A suitable control tissue is rat spinal cord or peripheral nerve homogenate.
Alternative Names:
14.3.3 ; Protein AS1; YWHAH; YWHA1; tyrosine 3-monooxygenase; tryptophan 5-monooxygenase activation protein 1;
Mouse anti-Nestin Monoclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells. The molecular weight of human Nestin as determined by SDS-PAGE mobility is about 240 kDa. However the real molecular weight is considerably less than this, at 177 kDa, the disparity being likely due to the highly charged region of the C-terminal segment. Nestin is relatively poorly conserved in protein sequence across species boundaries, so that the mouse and human proteins have an overall identity of only 62%. As a result antibodies to the human protein often fail to recognize the rodent homologue and vice versa. However this antibody stains both rodent and human Nestin. Antibodies to Nestin are widely used to identify neural stem cells.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS buffer pH 7.2-7.6 with 0.1% trehalose and 5 mM sodium azide as preservative.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Partial segment (region 317-630 aa) of human Nestin expressed in E.coli
Applications:
ICC,WB
Clone number:
4D11
Antibody Isotype:
IgG1, kappa
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Flow Cytometry. Suggested dilution for WB is 1:1,000-5,000 and 1:250-500 for IC. Use 2 ug/10^6 cells for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Nestin; NES;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody is specific for the 240 kDa Nestin protein by WB on developing rat brain (P18) homogenate. A much weaker band at approx. 90 kDa may also be seen. This is suggested to be a breakdown product of the 240 kDa band. Human, Rodent
Mouse anti-Glial fibrillary acidic protein (GFAP) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS, pH 7.4, containing 3% trehalose and 0.05% sodium azide.
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:5,000 is recommended for WB. Human GFAP has a predicted length of 432 residues and a MW of 50 kDa. A dilution of 1:500-1:1,000 is recommended for ICC/IHC. This antibody works well on frozen sections, cells in tissue culture and on formalin fixed histological sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Astrocyte; Glial fibrillary acidic protein; GFAP;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Reeves S.A, et al. Proc. Natl. Acad. Sci. U.S.A. 86:5178-5182(1989). 2. Brenner M, et al. Brain Res. Mol. Brain Res. 7:277-286(1990). Isaacs A, et al. Genomics 51:152-154(1998). Ota T, et al. Nat. Genet. 36:40-45(2004). Nielsen A.L, et al. J. Biol. Chem. 277:29983-29991(2002). Singh R, et al. Genomics 82:185-193(2003). Brenner M, et al. Nat. Genet. 27:117-120(2001). Brockmann K, et al. Eur. Neurol. 50:100-105(2003). Stumpf E, et al. Arch. Neurol. 60:1307-1312(2003). Sawaishi Y, et al. Neurology 58:1541-1543(2002). Aoki Y, et al. Neurosci. Lett. 312:71-74(2001).
Specificity:
The specificity of this antibody has been confirmed by WB. Human, Rat, Mouse, Bovine, Porcine. Predicted to react with other mammalian and avian species.
Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) due to its molecular mass and its ability to bind at low affinity not only NGF (see 162030), but also other neurotrophins, including brain-derived neurotrophic factor (BDNF; 113505), neurotrophin-3 (NTF3; 162660), and neurotrophin-4/5 (NTF5; 162662). At the time of its discovery, NGFR was considered a unique type of protein. Subsequently, however, a large superfamily of tumor necrosis factor receptors were found to share the overall structure of NGFR (4 extracellular ligand-binding, cysteine-rich repeats, or CRs, and signaling through association with, or disassociation from, cytoplasmic interactors). The identification of this superfamily helped elucidate some of the biologic functions of NGFR, including its ultimate involvement in the nuclear factor kappa-B (NFKB; see 164011) and apoptosis pathways. As a monomer, NGFR binds NGF with low affinity. Higher affinity binding is achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA (NTRK1; 191315), TRKB (NTRK2; 600456), and TRKC (NTRK3; 191316). TRKA, TRKB, and TRKC are specific for or 'preferred by' NGF, NTF5 and BDNF, and NTF3, respectively (Ip et al., 1993). NTF3 also binds to TRKA and TRKB, but with significantly lower affinity
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid (1 mg/mL) in PBS, pH 7.2-7.6 without preservatives. Typical Fluorophore/Protein (F/P) - ratio is 3-10.
Host Animal:
Mouse
Species Reactivity:
Cat,Dog,Human,Pig,Rabbit,Sheep
Immunogen:
The p75NTR antibody was derived from immunization of mice with human WM245 melanoma cells.
Applications:
ICC
Clone number:
ME20.4
Antibody Isotype:
IgG1, kappa
Application Details:
This antibody is recommended for use in immunohistochemistry, immunofluorescence, flow cytometry and NGF receptor p75 dynamics. For immunohistochemistry a concentration of 2 µg/mL is recommended. Not appropriate for Western Blots. For FACS a concentration of 20 µg/mL is recommended and for 1 site ELISA at least a 1 in 5000 dilution. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Walker BR et al. (2007) http://www.ncbi.nlm.nih.gov/pubmed/16971000?ordinalpos=24&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum'> Behav Brain Res 176:109-20 Ross A.H. et al. (1984) Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies. http://www.ncbi.nlm.nih.gov/pubmed/6093111?ordinalpos=24&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum'> Proc Natl Acad Sci U S A. 1984 Nov;81(21):6681-5. ORIGINAL PAPER
Specificity:
This antibody recognises p75NTR (low affinity neurotrophin receptor) Reacts with human, cat, dog, pig, rabbit and sheep. Does not react with rat or mouse.
Purification:
Protein G purified IgG was labelled with ATTO 488 and free dye removed by gel filtration.
Monoclonal antibody MC192 against the rat low affinity nerve growth factor receptor (p75NTR) is derived from the fusion of Sp2/0-Ag 14 myeloma cells with mouse immune splenocytes. MC192 monoclonal antibody was originally generated by Chandlers et al. p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. MC192 has a potential use as the ligand for gene delivery into p75NTR-expressing rat cells via a receptor-mediated mechanism. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1 (By similarity).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Host Animal:
Mouse
Species Reactivity:
Rat
Immunogen:
Rat p75NTR
Applications:
FC,ICC,IHC-Frozen,IHC-Paraffin-embedded
Clone number:
MC192
Antibody Isotype:
IgG1
Application Details:
IF: live or lightly fixed cells or tissues (acetone or 4% PFA): 2-5µg/mL. Not suitable for western blots; not suitable for IH on formalin fixed tissues. FACS (20µg/mL) is recommended, unfixed cells.
Chandler, C. E. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0006327698'> (1984) J Biol Chem 259, 6882-6889 Lagares A et al (2007) http://www.jneurosci.org/cgi/search?sendit=Search&pubdate_year=&volume=&firstpage=&author1=lagares=&title=&andorexacttitle=and&titleabstract=&andorexacttitleabs=and&fulltext=&andorexactfulltext=and&fmonth=Jan&fyear=1981&tmonth=Jan&tyear=2008&fdatedef=1+January+1981&tdatedef=16+January+2008&flag=&RESULTFORMAT=1&hits=10&hitsbrief=25&sortspec=relevance&sortspecbrief=relevance' > J of neurosci 27(30), 7939-7953
Specificity:
MC192 recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat and does not react with human or mouse NGFR. Reacts with rat. Does not react with mouse or human NGFR
Purification:
Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The IgG was then conjugated to ATTO 488 (ATTO TEC) and purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
Mouse anti-p75 neurotrophin receptor (p75NTR) Monoclonal Antibody (FITC), suitable for IHC-Frozen, ICC, FC.
Background Info:
Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) due to its molecular mass and its ability to bind at low affinity not only NGF (see 162030), but also other neurotrophins, including brain-derived neurotrophic factor (BDNF; 113505), neurotrophin-3 (NTF3; 162660), and neurotrophin-4/5 (NTF5; 162662). At the time of its discovery, NGFR was considered a unique type of protein. Subsequently, however, a large superfamily of tumor necrosis factor receptors were found to share the overall structure of NGFR (4 extracellular ligand-binding, cysteine-rich repeats, or CRs, and signaling through association with, or disassociation from, cytoplasmic interactors). The identification of this superfamily helped elucidate some of the biologic functions of NGFR, including its ultimate involvement in the nuclear factor kappa-B (NFKB; see 164011) and apoptosis pathways. As a monomer, NGFR binds NGF with low affinity. Higher affinity binding is achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA (NTRK1; 191315), TRKB (NTRK2; 600456), and TRKC (NTRK3; 191316). TRKA, TRKB, and TRKC are specific for or 'preferred by' NGF, NTF5 and BDNF, and NTF3, respectively. NTF3 also binds to TRKA and TRKB, but with significantly lower affinity.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid. 10mM Tris, 50mM NaCl
Host Animal:
Mouse
Species Reactivity:
Cat,Dog,Human,Pig,Rabbit,Sheep
Immunogen:
The p75NTR antibody was derived from immunization of mice with human WM245 melanoma cells.
Applications:
FC,ICC,IHC-Frozen
Clone number:
ME20.4
Antibody Isotype:
IgG1
Application Details:
This antibody is recommended for use in immunohistochemistry, immunofluorescence, flow cytometry and NGF receptor p75 dynamics. For immunohistochemistry a concentration of 2 µg/mL is recommended. Not appropriate for Western Blots. For FACS a concentration of 20 µg/mL is recommended and for 1 site ELISA at least a 1 in 5000 dilution. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Walker BR et al. (2007) http://www.ncbi.nlm.nih.gov/pubmed/16971000?ordinalpos=24&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum'> Behav Brain Res 176:109-20 Ross A.H. et al. (1984) Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies. http://www.ncbi.nlm.nih.gov/pubmed/6093111?ordinalpos=24&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum'> Proc Natl Acad Sci U S A. 1984 Nov;81(21):6681-5. ORIGINAL PAPER
Specificity:
This antibody recognises p75NTR (low affinity neurotrophin receptor) Reacts with human, cat, dog, pig, rabbit and sheep. Does not react with rat or mouse.
Purification:
Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The antibody was then conjugated to Fluorescein isomer 1 (FITC, Sigma). A minimum fluorescein: protein ratio of 3:1 is guaranteed. The conjugate was purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
Mouse anti-p75 neurotrophin receptor (p75NTR) Monoclonal Antibody (FITC), suitable for IHC-Frozen, ICC, FC.
Background Info:
Monoclonal antibody MC192 against the rat low affinity nerve growth factor receptor (p75NTR) is derived from the fusion of Sp2/0-Ag 14 myeloma cells with mouse immune splenocytes. MC192 monoclonal antibody was originally generated by Chandlers et al. p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. MC192 has a potential use as the ligand for gene delivery into p75NTR-expressing rat cells via a receptor-mediated mechanism. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Host Animal:
Mouse
Species Reactivity:
Rat
Immunogen:
Rat p75NTR
Applications:
FC,ICC,IHC-Frozen
Clone number:
MC192
Antibody Isotype:
IgG1
Application Details:
Immunohistochemistry, immunofluorescence, flow cytometry, NGF receptor p75 dynamics, retrograde transport studies, study of intracellular trafficking. Suggested working dilutions: For immunohistochemistry a concentration of 1-2 µg/mL is recommended. The antibody is not appropriate for Western Blots. The recommended concentration for FACS is 20 µg/mL and at least 1 in 5000 dilution is recommended for 1-site ELISA. Optimal working dilution should be determined by the end user. MC192 is not suitable as a blocking agent, although it has been incorrectly used for this purpose in many published works. The antibody was generated specifically by screening for monoclonals that had the ability to ENHANCE the binding of NGF, the natural ligand for p75. Therefore, this antibody is particularly unusual. The full details can be found in the original paper, which is listed on our datasheet (see Chandler et al, 1984). Biosensis recommends optimal dilutions/concentrations should be determined by the end user. The FITC version of MC192 is primarily targeted for FACS or IF applications on live or lightly fixed cells. Antibody will not work in traditional formalin fixed tissues.
Chandler, C. E. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0006327698'> (1984) J Biol Chem 259, 6882-6889 2. Lagares A et al (2007) http://www.jneurosci.org/cgi/search?sendit=Search&pubdate_year=&volume=&firstpage=&author1=lagares&author2=&title=&andorexacttitle=and&titleabstract=&andorexacttitleabs=and&fulltext=&andorexactfulltext=and&fmonth=Jan&fyear=1981&tmonth=Jan&tyear=2008&fdatedef=1+January+1981&tdatedef=16+January+2008&flag=&RESULTFORMAT=1&hits=10&hitsbrief=25&sortspec=relevance&sortspecbrief=relevance' > J of neurosci 27(30), 7939-7953
Specificity:
MC192 recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat. Reacts with rat. Does not react with mouse or human p75 NGFR
Purification:
Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The antibody was then conjugated to Fluorescein isomer 1 (FITC, Sigma). A minimum fluorescein: protein ratio of 3:1 is guaranteed. The conjugate was purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
Mouse anti-p75 neurotrophin receptor (p75NTR) Monoclonal Antibody (Unconjugated), suitable for IHC-Frozen, FC.
Background Info:
Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) due to its molecular mass and its ability to bind at low affinity not only NGF (see 162030), but also other neurotrophins, including brain-derived neurotrophic factor (BDNF; 113505), neurotrophin-3 (NTF3; 162660), and neurotrophin-4/5 (NTF5; 162662). At the time of its discovery, NGFR was considered a unique type of protein. Subsequently, however, a large superfamily of tumor necrosis factor receptors were found to share the overall structure of NGFR (4 extracellular ligand-binding, cysteine-rich repeats, or CRs, and signaling through association with, or disassociation from, cytoplasmic interactors). The identification of this superfamily helped elucidate some of the biologic functions of NGFR, including its ultimate involvement in the nuclear factor kappa-B (NFKB; see 164011) and apoptosis pathways. As a monomer, NGFR binds NGF with low affinity. Higher affinity binding is achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA (NTRK1; 191315), TRKB (NTRK2; 600456), and TRKC (NTRK3; 191316). TRKA, TRKB, and TRKC are specific for or 'preferred by' NGF, NTF5 and BDNF, and NTF3, respectively (Ip et al., 1993). NTF3 also binds to TRKA and TRKB, but with significantly lower affinity
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Host Animal:
Mouse
Species Reactivity:
Cat,Dog,Human,Pig,Rabbit,Sheep
Immunogen:
The p75NTR antibody was derived from immunization of mice with human WM245 melanoma cells.
Applications:
FC,IHC-Frozen
Clone number:
ME20.4
Antibody Isotype:
IgG1
Application Details:
Immunohistochemistry, immunofluorescence, flow cytometry. Suggested working dilutions: For Immunohistochemistry a concentration of 2 µg/mL is recommended. Antibody not appropriate for Western Blot. For FACS a concentration of 20 µg/mL is recommended. At least 1 in 5000 dilution is recommended for 1 site ELISAs. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Walker BR et al. (2007) http://www.ncbi.nlm.nih.gov/pubmed/16971000?ordinalpos=24&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum'> Behav Brain Res 176:109-20 Ross A.H. et al. (1984) Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies. http://www.ncbi.nlm.nih.gov/pubmed/6093111?ordinalpos=24&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum'> Proc Natl Acad Sci U S A. 1984 Nov;81(21):6681-5. ORIGINAL PAPER
Specificity:
This antibody recognises p75NTR (low affinity neurotrophin receptor) Reacts with human, cat, dog, pig, rabbit and sheep. Does not react with rat or mouse.
Purification:
Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS and then lyophilized. Purity was analysed using electrophoresis, 4-12% Bis Tris Gel (Invitrogen).
Monoclonal antibody MC192 against the rat low affinity nerve growth factor receptor (p75NTR) is derived from the fusion of Sp2/0-Ag 14 myeloma cells with mouse immune splenocytes. MC192 monoclonal antibody was originally generated by Chandlers et al. p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. MC192 recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat. MC192 antibody may be used for immunocytochemical localisation of rat cells expressing p75NTR, ELISA and western blot. This antibody has also been used for the construction of the MC192-saporin immunotoxin for specific elimination of neuronal populations in basal forebrain cholinergic neurons to generate an animal model for Alzheimer's disease. Using Flow Cytometry, this antibody has frequently been employed for panning to isolate p75NTR-expressing rat cells. MC192 has a potential use as the ligand for gene delivery into p75NTR-expressing rat cells via a receptor-mediated mechanism. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1 (By similarity). Interacts with BEX1. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. DOMAIN: Death domain is responsible for interaction with RANBP9. PTM: N- and O-glycosylated. PTM: Phosphorylated on serine residues. SIMILARITY: Contains 1 death domain. SIMILARITY: Contains 4 TNFR-Cys repeats.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Host Animal:
Mouse
Species Reactivity:
Rat
Immunogen:
NGF receptor
Applications:
ELISA,IHC-Frozen,WB
Clone number:
MC192
Antibody Isotype:
IgG1
Application Details:
IH (lightly fixed), ELISA, WB, Flow Cytometry (2 ug per 10^6 cells) IP (non-reducing conditions only!; do not use reducing agents such as DTT or beta-mercaptoethanol), Traditional formalin fixed paraffin embedded immunohistochemistry is NOT recommended with MC192. Motor neuron isolation, Gene/Toxin Delivery to rat sensory/motor neurons. A working solution of 1-2 µg/mL was determined by immunohistochemical staining on 4% paraformaldehyde fixed, or alcohol fixed rat spinal cord and brain. For non-denatured WB, 1-5 µg/mL was found to be suitable with suitable controls (PC12 lysate). ELISA: detection only, 1-5 µg/mL has been suggested in literature.Immunoprecipitation: 5 µg/mL, > 0.5% triton X-100 buffer/500 ug/lysate; PC12 positive control strong suggested. MC192 is not suitable as a blocking agent, although it has been incorrectly used for this purpose in many published works. The antibody was generated specifically by screening for monoclonals that had the ability to ENHANCE the binding of NGF, the natural ligand for p75. Therefore, this antibody is particularly unusual. The full details can be found in the original paper, which is listed on our datasheet (see Chandler et al, 1984). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Vilar M. et al (2009) Activation of the p75 neurotrophin receptor through conformational rearrangement of disulphide-linked receptor dimers http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=19376068'> Neuron. 2009 Apr 16;62(1):72-83 Luther, J.A. and Birren, S.J. (2009) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=19403809'> J. Neurosci 29, 5411-24 Lagares, A. et al. (2007) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=17652585'> J. Neurosci 27, 7939-53 Kruger, G. M. et al. (2002) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12194866'> Neuron 35, 657-69 Tuffereau, C. et al (1998) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0009857182'> Embo J 17, 7250-9 Zhou, X. F., and Rush, R. A. (1996) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0008841920' > J Comp Neurol 372, 37-48 Huber, J., Dittrich, F., and Phelan, P. (1993) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8281921' > Eur J Biochem 218, 1031-1039 Rabizadeh S. et al (1993) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8332899'> Science. Jul 16;261(5119):345-8. Stemple, D. L. & Anderson, D. J. (1992) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1458542'> Cell 71, 973-85 Urschel, B. A. & Hulsebosch, C. E. (1992) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0001424101'> Brain Res Dev Brain Res 69, 261-70 Birren SJ et al (1992) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1321502 '> Science. Jul 17;257(5068):395-7. Yan, Q., and Johnson, E. M., Jr. (1988) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2845023' > J Neurosci. 1988 Sep;8(9):3481-98. Johnson, D. et al. (1986) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3022937'> Cell 47, 545-54 Chandler, C. E. et al (1984) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=0006327698'> (1984) J Biol Chem 259, 6882-6889
Specificity:
MC192 is specific only for RAT NGFR, no reactivity to Human or Mouse NGFR has been reported This monoclonal antibody has been tested for immunohistochemical localisation of p75NTR-expressing rat cells in the spinal cord and brain. This monoclonal antibody does not cross react with p75NTR-expressing cells in other species.
p75NTR (CD271) was originally discovered as a low affinity nerve growth factor receptor (NGFR). Later it was found that it was the receptor for all neurotrophins, including NGF, BDNF, NT3 and NT4/5. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. Recent research proposes the extracellular domain of p75NTR as a biomarker for monitoring the progression of motor neuron disease (MND), also known as Amyotrophic Lateral Sclerosis (ALS) or Lou Gehrig's Disease. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.
Applications:
FC,ICC,IHC-Frozen,Immunopanning,IP,WB
Clone number:
8J2
Antibody Isotype:
IgG2a
Application Details:
Flow Cytometry: 5-20 µg/mL. Western Blotting: 0.5-2.0 µg/mL, non-reducing conditions only (no DTT or beta-mercaptoethanol). Immunoprecipitation: lysate dependent. 10 ug per 200-500 ug total protein. Immunopanning: 1-5 µg/mL. Immunocytochemistry: 1-5 µg/mL. Staining is strongest in non-fixed cells, light fixation is tolerable. Immunohistochemistry: fresh, acetone fixed sections only, epitope is fixation sensitive. Not suitable in formalin-fixed, paraffin (FFPE) embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Matusica D et al. (2008). Characterisation and use of the NSC-34 cell liner for study of nerurotrophin receptor trafficking. <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/17896795?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum"> J. Neurosci. Res. 86(3) pp. 553-65. Huh CY et al. (2008). Chronic exposure to nerve growth factor increases acetylcholine and glutamate release from cholinergic neurons of the rat medial septum and diagonal band of Boca via mechanisms mediated bu p75NTR.<a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/18256260?ordinalpos=3&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum"> J. Neurosci. 28(6) pp. 1404-9. Lagares A et al. (2007). Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio-neuronal precursor maturation. <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/17652585?ordinalpos=6&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum"> J. Neurosci. 27(30) pp. 7939-53. DiStefano & Johnson (1988). Identification of a truncated form of the nerve growth factor receptor. <a class="newA" target="_blank" href="https://www.ncbi.nlm.nih.gov/pubmed/2829174"> Proc Natl Acad Sci U S A. 1988 Jan;85(1):270-4.
Specificity:
Human, reacts with human, mouse and rat. Cross-reactivity with other species not tested but expected.This antibody is specific for NGFR/p75NTR as demonstRated by western blotting and immunprecipitation. The antibody recognizes extracellular p75NTR under non-reducing conditions.
Mouse anti-Semaphorin-5A Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Members of the semaphorin protein family, such as Semaphorin 5A, are involved in axonal guidance during neural development. Semaphorin 5A acts as a bifunctional guidance cue, exerting both attractive and inhibitory effects on developing axons of the fasciculus retroflexus (FR), a diencephalon fiber tract associated with limbic function. Neuronal responses to Semaophorin 5A are dependent on the bound proteoglycan and the biologic context. Mammalian plexin B3 is a high-affinity Semaphorin 5A receptor.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 50% glycerol
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human Semaphorin 5A (393-499) with a GST tag.
Applications:
ELISA,WB
Antibody Isotype:
Mixed
Application Details:
This antibody is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Semaphorin F; Sema F; SEMA5A; SEMAF
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Mouse anti-Neurotrophin-3 (NT-3) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
NT3 is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It seems to promote the survival of visceral and proprioceptive sensory neurons. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. NT3 is a secreted protein that is expressed in brain and peripheral tissues.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 50% glycerol
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human NT3 (19-128) with a GST tag.
Applications:
ELISA,WB
Antibody Isotype:
Mixed
Application Details:
This antibody is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Mouse anti-Nuclear receptor subfamily 2 group E member 1 (TLX) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Nuclear receptor TLX is an orphan receptor that binds DNA as a monomer and shares a target gene specificity that is unique among the nuclear receptor superfamily. It is thought to be required for brain development and also involved in the regulation of retinal development. It is a brain-specific protein that is present in all brain section tested with highest levels in the caudate nucleus and hippocampus.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Partial recombinant human Nuclear receptor TLX [248-342] with a GST tag.
Applications:
ELISA,WB
Clone number:
1C4
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for WB, ELISA and Flow Cytometry (~2 µg per 10^6 cells). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Nuclear receptor subfamily 2 group E member 1; Protein tailless homolog; TII; hTII; NR2E1; TLX
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by direct ELISA against the antigen. Human, mouse and rat. Other species have not been tested.
Purification:
Protein G purified immunoglobulin
Target:
Nuclear receptor subfamily 2 group E member 1 (TLX)
Mouse anti-Semaphorin-6C Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Semaphorin 6C is a transmembrane protein that shows growth cone collapsing activity on dorsal root ganglion neurons in vitro. It may be a stop signal for the dorsal root ganglion neurons, and possibly other neurons, in their target areas. It may also be involved in the maintenance and remodeling of neuronal connections.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 50% glycerol
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human Semaphorin 6C (27-126) with a GST tag.
Applications:
ELISA,WB
Antibody Isotype:
Mixed
Application Details:
This antibody is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Semaphorin Y; Sema Y; SEMA6C; KIAA1869; SEMAY
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Mouse anti-Dihydropyrimidinase-related protein 4 (DRP-4) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
DRP-4 belongs to the hydantoinase/dihydropyrimidase subfamily. It is required for signalling by class 3 semaphorins and remodelling of the cytoskeleton. It also plays a role in axon guidance, neuronal growth cone collapse and cell migration. It exists as homotetramer and a heterotetramer. Antibodies against post-translationally modified DPR-4 are present in sera from patients with paraneoplastic neurological diseases.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 50% glycerol
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human DRP-4 (461-559) with a GST tag.
Applications:
ELISA,WB
Antibody Isotype:
Mixed
Application Details:
This antibody is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Dihydropyrimidinase-relate protein 3; Collapsin response mediator protein 3; CRMP-3; UNC33-like phosphoprotein 4; ULIP4 protein; DPYSL4; CRMP3; ULIP4
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Mouse anti-Slit homolog 2 protein (Slit-2) Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Slit-2 is thought to act as molecular guidance cue in cellular migration, and its function appears to be mediated by interaction with roundabout homolog (ROBO) receptors. It is a secreted protein known for its role of repulsion in axon guidance and neuronal migration that can also inhibit leukocyte chemotaxis induced by chemotactic factors.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 50% glycerol
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human Slit-2 (1361-1459) with a GST tag.
Applications:
ELISA,WB
Antibody Isotype:
Mixed
Application Details:
This antibody is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Slit homolog 2 protein; SLIT2; SLIL3
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
It has been demonstrated that TrpC1 and TrpC5 are subunits of a heteromeric neuronal channel in mammalian brain. TrpC5 is thought to form a receptor-activatd non-selective permeant cation channel. It is probably operated by a phosphatidylinositol second messenger system activated by receptor kinases or G-protein coupled receptors. It has also been shown to be calcium-selective and it may be also activated by intracellular calcium store depletion. TrpC5 protein was shown to be present in hippocampal neuronal growth cones, neuronal cell bodies, areas consistent with new synapse formation, and cytoplasmic transport vesicles along neuronal processes. TrpC5 is expressed in brain with higher levels in fetal brain. Influxes of calcium via voltage-gated channels play a role in neuronal outgrowth and it was suggested that TrpC5 is a candidate for the regulation of calcium waves.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. Storage buffer: 50% glycerol
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human TrpC5 [534-603] with a GST tag.
Applications:
ELISA,WB
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Short transient receptor potential channel 5; Htrp-5; Htrp5; TRPC5; TRP5
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by ELISA against the antigen. Human. Other species have not been tested.
Purification:
Purified
Target:
Short transient receptor potential channel 5 (TrpC5)
Mouse anti-Myelin-associated oligodendrocyte basic protein (MOBP) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
MOBP may play a role in compacting or stabilising the myelin sheath, possibly by binding the negatively charged acidid phospholipids of the cytoplasmic membrane. It is present in the major dense line of CNS myelin. There are 3 named isoforms produced by alternative splicing. MBP is abundantly expressed specifically in oligodendrocytes at the mRNA level. It is expressed exclusively in the CNS myelin.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human MOBP, isoform 3 [1-82], with a GST tag. Isotype is IgM Kappa.
Applications:
ELISA,WB
Clone number:
1H3
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Myelin-associated oligodendrocyte basic protein
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by WB and ELISA against the antigen. Human. Other species have not been tested.
Purification:
Ascites fluid
Target:
Myelin-associated oligodendrocyte basic protein (MOBP)
Mouse anti-Semaphorin-4A Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Semaphorin 4A is a transmembrane protein that inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Defects in Semaphorin 4A cause retinitis pigmentosa which leads to degeneration of retinal photoreceptor cells. Defects in Semaphorin 4A are also the cause of cone-rod dystrophy type 10.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 50% glycerol
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human Semaphorin 4A (33-132) with a GST tag.
Applications:
ELISA,WB
Antibody Isotype:
Mixed
Application Details:
This antibody is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Semaphorin B; Sema B; SEMA4A; SEMAB; SEMB
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Mouse anti-Semaphorin-4G Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. There are eight classes of Semaphorins. Semaphorin 4G is a transmembrane protein that may play a role in axon guidance. It belongs to the semaphorin family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 50% glycerol
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human Semaphorin 4G (24-115) with a GST tag.
Applications:
ELISA,WB
Antibody Isotype:
Mixed
Application Details:
This antibody is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
SEMA4G; KIAA1619
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Protein phosphatase 1, regulatory subunit 9A is 132 amino acid protein with an estimated molecular weight of ~148 kDa. It is thought to be involved in neurite formation.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Partial recombinant human Protein phosphatase 1, regulatory subunit 9A [1090-1188) with a GST tag.
Applications:
ELISA,WB
Clone number:
6A10
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Mouse anti-Semaphorin-3A Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Semaphorin-3A is a secreted protein that induces the collapse and paralysis of neuronal growth cones. It could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. It Binds to the complex neuropilin-1/plexin-1. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.4
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human Semaphorin 3A (672-770) with a GST tag.
Applications:
ELISA,WB
Clone number:
5G9
Antibody Isotype:
IgG2a, kappa
Application Details:
This antibody is recommended for WB, and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Semaphorin III; Sema III; SEMA3A; SEMAD
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Mouse anti-Neuropilin-1 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
The antibody is made against the membrane-bound isoform 1 of Neuropilin-1. It is a receptor involved in the development of the cardiovascular systerm, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It binds both vascular endothelial growth factor (VEGF) and semaphorin family members. Isoform 1 is expressed by blood vessels of different tissues. In the developing embryo it is predominantly found in the nervous system. In adult tissues, it is highly expressed in heart and placenta, moderately expressed in lung, liver and skeletal muscle, kidney and pancreas, and low in adult brain.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.4
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human Neuropilin-1 (22-131) with a GST tag.
Applications:
ELISA,WB
Clone number:
1B3
Antibody Isotype:
IgG2a, kappa
Application Details:
This antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Mouse anti-Ectoderm-heural cortex protein 1 (ENC-1) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
ENC-1 is an actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. It is a substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target protein. It is detected in fetal brain tissue, moderate expression in fetal heart, lung and kidney. It is highly exprssed in adult brain, especially in the hippocampus and amygdala, and spinal cord. It is dramatically up-regulated upon neuronal differentiation.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human ENC-1 (amino acids 17-98) with a GST tag.
Applications:
ELISA,WB
Clone number:
3B1
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Ectodermal-neural cortex protein 1; p53-induced gene 10 protein; Nuclear matrix protein NRP/B; ENC1; NRPB; PIG10
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Mouse anti-Protein S100-A1 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Protein S100-A1 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Protein S100-A1 exists as a dimer of either two alpha chains, two beta chains or one alpha and one beta chain. It weakly binds calcium but binds zinc very tightly and distinct binding sites with different affinities exist for both ions on each monomer. This protein is highly prevalent in heart and to a lesser extent in skeletal muscle and brain.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human Protein S100-A1 (amino acids 1-75) with a GST tag.
Applications:
ELISA,WB
Clone number:
1D5
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for WB and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
S100 calcium-binding protein A1 S-100 protein alpha subunit; S-100 protein alpha chain; Roundabout homolog 1; S100A1; S100A
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Roundabout homolog 1 belongs to the neural adhesion molecule (NCAM) family of receptors. It is a receptor for SLIT1 and SLIT2 which are thought to act as a molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. It may be required for lung development. There are three named isoforms. It is widely expressed, with the exception of kidney. Defects in Roundabout homolog 1 may be a cause of breast and lung cancer.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human Roundabout homolog 1 (amino acids 491-589) with a GST tag.
Applications:
ELISA,WB
Clone number:
2G6
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for WB and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
H-Robo-1; Deleted in U twenty twenty; ROBO1; DUTT1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Mouse anti-Paired box protein Pax-5 Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA.
Background Info:
Paired box protein Pax-5 is a member of the paired box (PAX) family of transcription factors. This family has a highly conserved DNA-binding motif known as the paired box. Paired box protein Pax-5 may play an important role in B-cell differentiation as well as neural development and spermatogenesis. It is involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene. It localises to the nucleus and is expressed at early B-cell differentiation in the developing CNA and in adult testis. A defect in Paired box protein Pax-5 is a cause of acute lymphoblastic leukemia.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human Paired box protein Pax-5 (192-302) with a GST tag.
Applications:
ELISA,IHC-Frozen,WB
Clone number:
8F9
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for WB, IHC and sandwich ELISA. The recommended dilution for this antibody is 1 µg/mL for IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
B-cell-specific transcription factor; BSAP; PAX5
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Mouse anti-Tyrosine Kinase Receptor B (TrkB) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Tyrosine kinase receptor type B (TrkB) is a member of the neurotrophic tyrosine receptor kinase family. It is a membrane-bound receptor and upon neurotrophin binding, it phosphorylates itself as well as MAPK pathways members. TrkB is the receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5, but not nerve growth factor (NGF). It is involved in the development and/or maintenance of the nervous system. It exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. It is a tran-membrane protein. There are 3 named isoforms produced by alternative splicing. Additional isoforms seem to exist. The different forms are differentially expressed in various cell types including neurons and microglia.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant protein of human Tyrosine kinase receptor type B (isoform TrkB-T1) with a GST tag.
Applications:
ELISA,WB
Clone number:
4D3-F10
Antibody Isotype:
IgG2a, kappa
Application Details:
This antibody is recommended for WB, sandwich ELISA and Flow Cytometry. Use 2 ?g/10^6 cells for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Mizoguchi Y et al., J Immunol. 2009 Dec 15;183(12):7778-86. Spencer-Segal JL et al. J Neurosci. 2011 May 4;31(18):6780-90. Nakajima K et al. Glia. 1998 Nov;24(3):272-89.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Mouse anti-Neurogenin-1 (NGN-1) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Neurogenin-1 appears to mediate neuronal differentiation. It requires dimerisation with another basic-helix-loop-helix protein for efficient DNA binding. Its expression is restricted to the embryonic nervous system.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human Neurogenin-1 (82-164) with a GST tag.
Applications:
ELISA,WB
Clone number:
4A2
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for WB, sandwich ELISA and immunofluorescence. The recommended dilution for this antibody is 50 µg/mL for immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Mouse anti-Neurofilament heavy polypeptide (NF-H) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Neurofilaments contain three intermediate filament proteins: L, M and H, which are involved in the maintenance of the neuronal caliber. NF-H has an important function in mature axons. NF-H is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Defects in NF-H are a cause of amyotrophic lateral sclerosis (ALS) which is a neurodegenerative disorder affecting upper and lower motor neurons and results in fatal paralysis.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human NF-H protein (263-364) with a GST tag.
Applications:
ELISA,WB
Clone number:
2.00E+08
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for WB and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; NEFH; KIAA0845; NFH
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Purification:
Protein G purified immunoglobulin
Target:
Neurofilament heavy polypeptide (NF-H)
Accession Number:
P12036
Research Areas:
Antibodies & Conjugates/Biosensis Stem Cells/Antibody to Neurofilament
LIMK-1 belongs to a small subfamily of LIM proteins with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 regulates actin filament dynamics. It phoshorylates and inactivates the actin binding/ depolymerising factor cofilin, consequently stabilising the actin cytoskeleton. It may also be involved in brain development. LIMK-1 localises to the cytoplasm and is highly expressed in both the adult and fetal nervous system. It is expressed to a lesser extent in heart and skeletal muscle. LIMK-1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant protein of human LIMK-1 (aa 548 to 648) with a GST tag.
Applications:
ELISA,WB
Clone number:
1A8
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for WB, IHC and sandwich ELISA. For IHC, a concentration of 3 µg/mL is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
LIM domain kinase 1; LIMK1; LIMK
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Mouse anti-LIM homeobox protein 1 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
LIM homeobox protein 1 is a potential transcription factor that may play a role in differentiation and development of neural and lymphoid cells. It contains 1 homeobox DNA-binding domain and 2 LIM zinc-binding domains. The latter have a negative regulatory function and disruption of the LIM domains results in an activated form. It localises to the nucleus and is expressed in the brain, thymus and tonsils. It is also expressed in samples from patients with chronic myeloid leukemia and in 58% of acute myeloid leukemia cell lines.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant protein of human LIM homeobox protein 1 (aa 1 to 101) with a GST tag.
Applications:
ELISA,WB
Clone number:
3G3
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
LIM/homeobox protein Lhx1; Homeobox protein 1; hLim-1; LHX1; LIM-1; LIM1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Transcription factor HES-1 is a transcriptional repressor of genes that require a basic helix-loop-helix protein for their transcription. It needs to form a complex with a corepressor protein (Groucho) to repress transcription. Transcription factor HES-1 has a particular type of basic domain which contains a helix-interrupting proline, that binds to the N-box (CACNAG) rather than the canonical E-box (CANNTG). It also consists of a C-terminal WRPW motif that is necessary for the interaction with Groucho. It localises to the nucleus.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.3
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant protein of human Transcription factor HES-1 (aa 36 to 143) with a GST tag.
Applications:
ELISA,WB
Clone number:
4D9
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Hairy and enhancer of split 1; Hairy-like; HHL; Hairy homolog; HES1; HL; HRY
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Mouse anti-Homeobox protein GBX-2 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Homeobox protein GBX-2 may act as a transcription factor for pluripotency and differentiation in the embryo. It localises to the nucleus. The unprocessed precursor is 348 amino acids long and has an estimated molecular weight of 37 kDa.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant protein of human Homeobox protein GBX-2 (aa 114 to 182) with a GST tag.
Applications:
ELISA,WB
Clone number:
2A4
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for WB and ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Gastrulation and brain-specific homeobox protein 2; GBX2
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Mouse anti-Ephrin-A51 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Ephrin-A5 is a member of the ephrin (EPH) gene family. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Ephrin-A5 prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human,Mouse
Immunogen:
Partial recombinant protein of human Ephrin-A5 (aa 114 to 204) with a GST tag.
Applications:
ELISA,WB
Clone number:
1F12
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Protein kinase minibrain homolog phosphorylates serine, threonine and tyrosine residues in its sequence as well as in exogenous substrates. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. There are 5 named isoforms produced by alternative splicing. It is expressed ubiquitously with highest levels in skeletal muscle, testis, fetal lung and kidney, and in the developing central nervous system. It localises to the nucleus speckle. This protein is overexpressed 1.5-fold in fetal Down syndrome brain and considered a strong candidate for learning defects associated with Down syndrome. It belongs to the protein kinase superfamily, CMGC ser/Thr protein kinase family and MNB/DYRK subfamilly.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Partial recombinant protein of human protein kinase minibrain homolog (aa 674 to 763) with a GST tag.
Applications:
ELISA,WB
Clone number:
7D10
Antibody Isotype:
IgG
Application Details:
Direct and sandwich ELISA and WB are recommended for this antibody. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity has been confirmed by WB and direct ELISA against the antigen. This antibody cross reacts with mouse and rat protein kinase minibrain homolog proteins.
Mouse anti-Chrondroitin sulfate proteoglycan 5/Neuroglycan C Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Chondrotin sulfate proteoglycan 5 may act as a growth and differentiation factor in neuritogenesis. It may also induce ERBB3 activation. It is expressed in the brain only and localises to the cell membrane; endoplasmic reticulum and golgi membranes; and in neurons to the synaptic junctions. This protein is N-glycosylated, O-glycosylated and phosphorylated. There are three isoforms produced by alternative splicing. A number of forms with varying molecular weights have been observed for this protein. This is possibly the result of differences in glycosylation, phosphorylation and protein cleavage.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human chondrotin sulfate proteoglycan 5 (amino acids 445 to 539 of isoform 2) with a GST tag
Applications:
ELISA,WB
Clone number:
5C11
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for WB and direct ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity has been shown by WB and ELISA against the GST tagged partial recombinant chondroitin sulfate proteoglycan 5 protein. No other species have been tested
Mouse anti-Cysteini-rich motor neuron 1 protein (CRIM1) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ELISA.
Background Info:
CRIM1 may participate in CNS development by interacting with growth factors involved in motor neuron differentiation and survival. It also has a potential role in capillary formation and maintenance during angiogenesis. It also affects BMP processing and delivery to the cell surface. CRIM1 interacts with BMP4 and BMP7. It localises to the cell membrane and is expressed in a number of tissues including the pancreas, kidney, skeletal muscle, lung, placenta, brain, heart, spleen, liver, small intestine and blood vessels. The unprocessed precursor has a length of 1036 amino acids and a predicted molecular weight of 113.7KDa. CRIM1 contains four antistatin-like domains, one IGFBP N-terminal domain and six VWFC domains. It is also N-glycosylated.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
A partial recombinant human CRIM1 protein (amino acids 36 to 145) with a GST tag.
Applications:
ELISA,IHC-Frozen,WB
Clone number:
6.00E+04
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for IHC at a dilution of 3 µg/mL. It is also recommended for sandwich ELISA with a detection limit for the recombinant GST tagged CRIM1 protein of 0.03 ng/mL. This antibody is useful for WB analysis. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Cysteine-rich motor neuron 1 protein; cysteine-rich repeat-containing protein S52; CRIM1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB and ELISA against the antigen. No other species have been tested.
Epidermal growth factor-like 1 belongs to the G-protein coupled receptor 2 family and the LN-TM7 subfamily. The unprocessed precursor has a length of 3312 amino acids and a predicted molecular weight of 358.3 KDa. It contains nine cadherin domains, eight EGF-like domains, one GPS domains, one laminin EGF-like domains and two laminin G-like domains. It is a transmembrane protein and is thought to have an important role in cell to cell signaling during nervous system formation.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
A partial recombinant protein of human Epidermal growth factor-like 1 (amino acids 71 to 180) with a GST tag.
Applications:
ELISA,WB
Clone number:
2F7
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for WB and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody was demonstrated by WB and ELISA against the GST tagged recombinant Epidermal growth factor-like 1 protein. This antibody has not been tested against other species.
Mouse anti-Calcium/calmodulin-dependent protein kinase 1 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
CaM kinase I belongs to a calcium-triggered signalling cascade involved in trasncriptional regulation, hormone production, translational regulation, regulation of actin filament organisation and neurite outgrowth. CaM kinase I is involved in calcium-dependent activation of the ERK pathway. It phosphorylates proteins with the substrate consensus sequence [MVLIF}-x-R-x(2)-[ST]-x(3)-[MVLIF]. It is activated by calcium ions or calmodulin. It reaches its maximum catalytic activity when phosphorylated by CAMKK1 or CAMKK2. CaM kinase I has a length of 370 amino acids (unprocessed precursor) and a predicted molecular weight of 41.3KDa. It is a monomer that exists in many tissues and is located within the cytoplasm or nucleus. It belongs to the protein kinase superfamily, the CAMK Ser/Thr protein kinase family and the CaMK subfamily.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant protein of human CaM kinase 1 (amino acids 271 to 370) with a GST tag.
Applications:
ELISA,WB
Clone number:
3G1
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for WB and both direct and sandwich ELISA. This antibody has a detection limit of 0.3ng/mL for recombinant GST tagged CaM kinase 1 when used in sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Calcium/calmodulin-dependent protein kinase type 1; CaM-KI; CaM kinase I alpha; CAMKI-alpha; CAMK1
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB and ELISA against the recombinant GST tagged CaM Kinase 1 protein. No other species have been tested yet.
Mouse anti-Fetal brain protein 239FB (239FB) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
The unprocessed precursor is 294 amino acids in length and has a predicted molecular weight of 33.6KDa. It belongs to the UPF0046 familly and is predominantly expressed in fetal brain.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
This antibody was raised agaisnt recombinant human 239FB protein (amino acids 1 to 294) with a GST tag.
Applications:
ELISA,WB
Clone number:
2G1-1B7
Antibody Isotype:
IgG
Application Details:
This antibody is recommended for use in WB and direct ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Metallophosphoesterase domain-containing protein 2; Fetal brain protein 239; MPPED2; C11orf8; FAM1B
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed in WB against the recombinant protein. Note the GST tag was used as a negative control. This antibody has not yet been tested against other species.
Mouse anti-Semaphorin-6A Polyclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
The transmembrane semaphorin Semaphorin 6A is expressed in developing neural tissue and is required for proper development of the thalamocortical neuron projection. Semaphorin 6A belongs to a subfamily characterized by an extracellular semaphorin domain, a transmembrane domain, and a long cytoplasmic tail. Members of this class can repel sympathetic and dorsal root ganglion axons in vitro, consistent with a traditional role as guidance signals. It was also suggested that Semaphorin 6A has a role in retrograde signaling.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 50% glycerol
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human Semaphorin 6A (21-120) with a GST tag.
Applications:
ELISA,WB
Antibody Isotype:
Mixed
Application Details:
This antibody is recommended for WB, ELISA and Flow Cytometry. Use 1:100 fold dilution for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human mature BDNF expressed in E.coli
Applications:
ELISA,ICC,WB
Clone number:
3C11
Antibody Isotype:
IgG
Application Details:
ELISA (50-100 ng/mL), WB (0.2 to 2 µg/mL), IF (2 to 20 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human mature BDNF expressed in E.coli
Applications:
ELISA,WB
Clone number:
4C8
Antibody Isotype:
IgG
Application Details:
ELISA: 50-100 ng/mL Western Blotting (denaturing and reducing): 0.2 to 2 µg/mL. M-1744-100 antibody detects 14 kDa mature BDNF monomer in human serum human and acid treated human and rodent whole tissue homogenates. In simple Tris-homogenate human brain lysates, M-1744-100 detects mature BDNF at 14kDa as well as an uncharacterized band at ~50kDa. No proBDNF band is detected in our samples, although M-1744-100 will detect proBDNF in purified form on blots. Additionally, M-1744-100 detects an additional band 18 kDa BDNF isoform human serum. For cell lysates, some caution is advised as in our hands M-1744-100 fails to detect mature BDNF on SH-SY5Y or C6 cell lysates but does detect mature BDNF in neuronal cell culture lysates. The reason for these differences has not been characterized. For detection of mature BDNF in cell lysates such as SH-SY5Y we recommend affinity-purified rabbit polyclonal antibody to rhBDNF (R-1707-100), or rabbit polyclonal antibody to BDNF peptide 1-10 (R-083-100, whole serum; R-066-500, IgG). Immunofluorescence: 1 to 4 µg/mL, 4% formaldehyde fixation and permeabilization. Staining can be weak until optimized. M-1744-100 is not recommended for Flow Cytometry at this time. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Mouse anti-Elastin Monoclonal Antibody (Unconjugated), suitable for IHC-Frozen, IHC-Paraffin-embedded.
Background Info:
Elastin is a major structural protein of tissues such as aorta and nuchal ligament. Elastin is formed through the cross-linking of its soluble precursor tropoelastin. At least 11 isoforms of human elastin are produced by alternative splicing.
Bovine alpha elastin. This antibody recognizes insoluble elastin, alpha elastin and tropoelastin.
Applications:
IHC-Frozen,IHC-Paraffin-embedded
Clone number:
IL-4
Antibody Isotype:
IgG
Application Details:
Immunohistochemistry (IHC-P). A concentration of 0.25-0.50 µg/mL is recommended to detect elastin in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Mouse anti-Desmin Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Background Info:
Desmin are class-III intermediate filaments expressed in smooth, cardiac and heart muscles. Desmin homopolymers form a stable fibrous network connecting myofibrils to each other and to the plasma membrane.
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 2.0 µg/mL is recommended for WB. Human Desmin has a predicted length of 470 residues and MW of 54 kDa. A concentration of 2.0-4.0 µg/mL is recommended to detect Desmin in formalin fixed and paraffin embedded tissues. Heat retrieval is necessary. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
DES; Desmin; CSM1; CSM2; CMD1I;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
Vascular endothelial growth factor (VEGF) receptor 2 is a type III receptor tyrosine kinase. As a receptor for VEGF, it mediates VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting.
Recombinant human extracellular domain of Vascular endothelial growth factor receptor 2
Applications:
IHC-Frozen,IHC-Paraffin-embedded,WB
Clone number:
FLK-6
Antibody Isotype:
IgG
Application Details:
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human VEGF receptor 2 has a predicted length of 1,356 residues and MW of 152 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin/acetone fixed frozen tissues.
Integrin alpha-6/beta-1 (ITGA6:ITGB1) is a receptor for laminin on platelets (By similarity). Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-6/beta-4 (ITGA6:ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464). Source: uniprot.org. In new work published by L.Barbar [PPR83158] the authors demonstrate that CD49f is an effective marker effective marker for sorting functional human astrocytes from mixed cultures.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
E.coli-derived human Integrin alpha 6/ITGA6 (CD49f) recombinant protein (amino acids Q189-K917).
Applications:
ELISA,WB
Antibody Isotype:
IgG
Application Details:
Western blotting (0.1-0.5 µg/mL) and direct ELISA (0.1-0.5 µg/mL). Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Integrin alpha-6; CD49 antigen-like family member F; VLA-6; CD_antigen: CD49f
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Rabbit IgG polyclonal antibody for Integrin alpha 6/ITGA6 detection. Tested in WB, and direct ELISA against Human, Mouse, and Rat extracts.
Tropomyosins are dimeric coiled coil proteins associated with the actin filaments of myofibrils and stress fibres. In vertebrates, at least 4 known genes code for diverse Tropomyosin isoforms that are expressed in a tissue-specific manner.
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Chicken Tropomyosin alpha-1 (isoform #1) has a predicted length of 284 residues and a MW of 33 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin/acetone fixed frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rabbit anti-Tyrosine Kinase Receptor C (TrkC) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation (Ref: uniprot.org).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from a solution containing 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic peptide immunogen, QLWQEQGEAKLNSQN from the ECD of human TrkC, amino acids 172-186.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (0.1 - 0.5 µg/mL): Tested on RIPA lysates from human and rodent tissue and cell lysates. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human TrkC, and Mouse and rat TrkC. Species cross-reactivity determined by Western Blotting. No Cross reactivity by WB seen with TrkA or TrkB receptors.
Actinin is a F-actin cross linking protein and is thought to anchor actin to a variety of intracellular structures. At least 4 isoforms of alpha actinin have been found. This antibody reacts specifically with alpha actinin isoform #1.
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 3.5 µg/mL is recommended for WB. The predicted length of human actinin (isoform #1) is 892 residues and a MW of 103 kDa. A concentration of 3.5-7.0 µg/mL is recommended to detect alpha actinin in formalin fixed and paraffin embedded tissues. Antigen retrieval is required. Also the recommended titre for formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
ACTN1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
Mouse anti-Synaptophysin Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Background Info:
Synaptophysin is a 38 kDa glycoprotein present in the membranes of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions and endocrine cells. It is also expressed by neuroendocrine cells, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms.
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Rat synaptophysin has a predicted length of 307 residues and a MW of 38 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect synaptophysin in formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Syp; p38; SVP38; SP; SYN; SYP1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Rat
Proliferating cell nuclear antigen (PCNA) belongs to the DNA sliding clamp family. PCNA helps increase the processivity of DNA polymerase during leading strand synthesis in DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway.
Protein A-PCNA fusion obtained from rat liver tissue.
Applications:
IHC-Frozen,IHC-Paraffin-embedded,WB
Clone number:
IML-83
Antibody Isotype:
IgG
Application Details:
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 2.0 µg/mL is recommended for WB. Human PCNA has a predicted length of 261 residues and MW of 29 kDa. A concentration of 0.4-1.0 µg/mL is recommended to detect PCNA in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Antigen retrieval may improve staining. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
PCNA; Cyclin;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat
Mouse anti-Neurofilament heavy polypeptide (NF-H) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Background Info:
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. This antibody recognises both phosphorylated and dephosphorylated forms of NF200/NF-H.
C-terminal segment of enzymatically dephosphorylated pig Neurofilament Heavy (NF200).
Applications:
IHC-Frozen,IHC-Paraffin-embedded,WB
Clone number:
G2/NF200/N52
Antibody Isotype:
IgG
Application Details:
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.50 µg/mL is recommended for WB. Human NF200 has a predicted length of 1026 residues and MW of 112 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect NF200 in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
Mouse anti-Glial fibrillary acidic protein (GFAP) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Background Info:
GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas. At least 3 isoforms are produced from alternate splicing. These isoforms differ in the C-terminal region which is encoded by alternative exons. This antibody detects all three isoforms.
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human GFAP has a predicted length of 432 residues and MW of 50 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect GFAP in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Astrocyte; Glial fibrillary acidic protein;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Reeves S.A, et al. Proc. Natl. Acad. Sci. U.S.A. 86:5178-5182(1989). Brenner M, et al. Brain Res. Mol. Brain Res. 7:277-286(1990). Isaacs A, et al. Genomics 51:152-154(1998). Ota T, et al. Nat. Genet. 36:40-45(2004). Nielsen A.L, et al. J. Biol. Chem. 277:29983-29991(2002). Singh R, et al. Genomics 82:185-193(2003). Brenner M, et al. Nat. Genet. 27:117-120(2001). Brockmann K, et al. Eur. Neurol. 50:100-105(2003). Stumpf E, et al. Arch. Neurol. 60:1307-1312(2003). Sawaishi Y, et al. Neurology 58:1541-1543(2002). Aoki Y, et al. Neurosci. Lett. 312:71-74(2001).
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
Mouse anti-Growth-associated protein 43 (GAP-43) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Background Info:
GAP-43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is also a major component of neuronal growth cones. GAP-43 is used to specifically label injured neurons and to score neuronal regeneration. This antibody reacts with the three known isoforms and to both kinase C phosphorylated and dephosphorylated forms of GAP-43.
GAP-43 protein from neonatal rat forebrain membranes
Applications:
IHC-Frozen,IHC-Paraffin-embedded,WB
Clone number:
GAP-8A12
Antibody Isotype:
IgG
Application Details:
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human GAP-43 has a predicted length of 238 residues and MW of 25 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect GAP-43 in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neuromodulin; GAP43; PP46; p57; B-50; F1; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; chicken; snake
Caldesmon is an actin and myosin binding protein. It plays a major role in the regulation of smooth muscle and non-muscle contractile events. At least 5 isoforms are known from alternate splicing. High molecular weight h-caldesmon (isoform 1) of 120-150 kDa is predominantly expressed in smooth muscles. Lower molecular weight caldesmon l-caldesmon (isoforms 2,3,4 and 5) of 61-70 kDa is more abundant in non-muscle tissues and cells. This antibody reacts specifically with h-caldesmon.
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human caldesmon has a predicted length of 793 residues and a MW of 93 kDa. A concentration of 2.0-4.0 µg/mL is recommended to detect caldesmon in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CAD; CDM; CALD1; CALD1_HUMAN; h-CD;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycaemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide (HSDPARRGELSVCDSISEWV) corresponding to a sequence at the N-terminal of human BDNF (amino acids 129-148).
Applications:
WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human BDNF has a predicted length of 247 residues and MW of 28 kDa for BDNF precursor and 13 kDa for mature BDNF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
A synthetic peptide (TVLGEIKTGNSPVKQY) corresponding to amino acid sequence 174-189 from human neurotrophin 3 (NT3).
Applications:
IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgG
Application Details:
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human NT3 has a predicted length of 257 amino acids and MW of 29 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect NT3 in paraffin embedded tissues. Antigen retrieval by boiling the paraffin / formalin section in 10mM citrate buffer, pH 6.0 for 20 minutes is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA). There are two known forms of GAD; GAD65 (65 kDa) and GAD67 (67 kDa) and several isoforms of GAD67 and GAD65 have been discovered. Clustal alignments show 64.1% amino acid identity between GAD65 and GAD67 across the entire protein with higher identity being toward the C-terminal ends of the proteins. Expression of the two proteins and of the specific isoforms (for instance isoform 3 of GAD67 is not expressed in brain) also varies. This antibody reacts with GAD67 and has not yet been tested directly on recombinant GAD65 for cross reactivity. Blots of whole brain extracts appear as a single band at 67 kDa. The sequence used as an immunogen does have some identity with GAD65 however it is slight and broken up by multiple gaps.
E.coli-derived human GAD67 recombinant protein (14-122). Human GAD67 shares 95% amino acid (aa) sequence identity with both mouse and rat GAD67.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. The detection limit for GAD67 is approximately 0.25ng/lane under reducing conditions. Human GAD67 has a predicted length of 594 residues and MW of 67 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.Not yet tested in other applications.
Alternative Names:
GAD; GAD-67; GAD1; Glutamate decarboxylase 1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB against HeLa cell, human colo320 cell and whole rat brain extracts. Antibody detects 67 kDa single band under normal exposure, reduced SDS-PAGE conditions. Human; Rat
Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. Three main groups of actin isoforms have been identified in vertebrates; alpha, beta and gamma. Alpha actins are found mainly in muscle tissues.
A synthetic peptide of 10 amino acids corresponding to the N-terminus of alpha smooth muscle actin. This antibody reacts only with alpha smooth muscle actin and does not react with actin from striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial). It also does not react with beta and gamma actins.
Applications:
IHC-Frozen,IHC-Paraffin-embedded,WB
Clone number:
1A4
Antibody Isotype:
IgG
Application Details:
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 2.0 µg/mL is recommended for WB. Human smooth muscle actin has a predicted length of 377 residues and a MW of 42 kDa. A concentration of 0.1-0.5 µg/mL is recommended to detect alpha smooth muscle actin in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
ACTA2; ACTSA; ACTVS;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
Rabbit anti-Tyrosine Kinase Receptor B (TrkB) Polyclonal Antibody (Unconjugated), suitable for WB, FC.
Background Info:
The protein named TrkB (also named Neurotrophic tyrosine kinase receptor type 2 (NTRK2), GP145-TrkB or Tropomyosin-related kinase B is a receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems and is important in the regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. TrkB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. TrkB is the primary receptor for BDNF (brain-derived neurotrophic factor. TrkB also binds NT4 and NT3 but less efficiently. Upon ligand-binding, the receptor undergoes homodimerization, autophosphorylation and activation. TrkB activation recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that each regulate distinct overlapping signaling cascades within cells. Through SHC1, FRS2, SH2B1, SH2B2, these activate the GRB2-Ras-MAPK cascade that regulates, for instance, neuronal differentiation including neurite outgrowth. These same effectors also control the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. TrkB, via activation of PLCG1 and the downstream protein kinase C-regulated pathways, also controls synaptic plasticity, and thus plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. One such consequence is that PLCG1 activation via TrkB is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. (Reference: www.uniprot.org)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from a solution containing 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3.
Western Blotting (0.1 - 0.5 µg/mL): Tested on RIPA lysates from human and rodent brain tissue. Detects TrkB full-length (~140 kDa) and truncated TrkB (~100 kDa) in tissue homogenates. In cell lysates, only a ~50 kDa uncharacterized TrkB isoform is detected. Flow Cytometry (5-20 µg/mL): Tested on human cell lines. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Trk-B,TrkB, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Positive Control:
Rat or mouse cortex (WB). SHSY5Y cell line (FC).
Specificity:
TrkB; detects TrkB in both human, mouse and rat samples by western blot and has positive staining by Flow cytometry against human TrkB positive cell lines.
Vascular endothelial growth factor (VEGF) is a disulfide linked homodimeric protein associated with angiogenesis, vasculogenesis and endothelial cell growth. At least 10 isoforms of VEGF are produced by alternative splicing with varying levels of expression. The isoforms VEGF189, VEGF165 and VEGF121 are widely expressed whereas the isoforms VEGF206 and VEGF145 are uncommon. Isoforms VEGF165 and VEGF121 are freely secreted whereas isoform VEGF189 remains cell associated.
A synthetic peptide corresponding to a sequence at the N-terminus of human VEGFA(33-48aa VDIFQEYPDEIEYIFK), identical to the related mouse and rat sequences.
Applications:
IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgG
Application Details:
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human Vascular endothelial growth factor (isoform VEGF206) has a predicted length of 232 residues and MW of 27 kDa. A concentration of 0.5-1 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; expected to react with mouse due to sequence homology
Rabbit anti-Prominin-1 Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded.
Background Info:
May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439). Ref: uniprot.org.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from a solution containing 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
E-coli derived human CD133/PROM1 recombinant protein (position: P531-H865) has been used as the immunogen. Human CD133 shares 61% amino acid sequence identity with mouse CD133.
Applications:
IHC-Paraffin-embedded,WB
Antibody Isotype:
IgG
Application Details:
Western Blot (0.1-0.5 µg/mL): tested in MCF-7 whole cell lysate. Immunohistochemistry: paraffin-embedded section (0.5-1.0 µg/mL) with HIER, pH 6.5; tested in human tonsil tissue. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Prominin-1, Prominin-like protein 1, PROM1, PROML1, Antigen AC133, MSTP061; CD133;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody is specific for CD133 as demonstrated by western blotting.
Purification:
Affinity purified
Target:
Prominin-1
Accession Number:
O43490
Research Areas:
Antibodies & Conjugates/Development,Antibodies & Conjugates/Biosensis Stem Cells,Antibodies & Conjugates/Biosensis Cancer
Collagen IV is a major constituent of the glomerular basement membranes. It is a heterotrimeric protein composed of 3 alpha subunits encoded by 6 different genes; alpha 1(IV) through alpha 6(IV).
Immunohistochemistry (IHC). A concentration of 0.5-1.0 µg/mL is recommended to detect Collagen IV in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Fibroblast growth factors (FGFs) bind heparin and exhibit widespread mitogenic and neurotrophic activities in a variety of different cells including mesenchymal, neuroectodermal and endothelial cells. FGF-4 is also known as Heparin secretory-transforming protein 1 (HST-1) and is reported to have mitogenic activity.
A synthetic peptide (RRLYCNVGIGFHLQ) corresponding to a region (84-97) from human FGF-4. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human FGF-4 (precursor) has a predicted length of 206 residues and MW of 22 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rabbit anti-Myelin basic protein (MBP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Background Info:
Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of approximately 80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP). MBP is an 'intrinsically unstructured' protein with a high proportion (approximately 75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system. A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational. Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic. The message for myelin basic protein is selectively translocated to the ends of the cell processes. Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model. FUNCTION: Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity). SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Myelin membrane; peripheral membrane protein; cytoplasmic side. Cytoplasmic side of myelin. TISSUE SPECIFICITY: Found in both the central and the peripheral nervous system.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3 added as preservative
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse (Predicted),Rat
Immunogen:
A synthetic peptide corresponding to a region (289-304 aa) from the C-terminus of human Myelin Basic Protein (MBP). This sequence also corresponds to a region on rat MBP (180-195 aa).
Applications:
IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgG
Application Details:
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human MBP (isoform #1, Golli-MBP1) has a predicted length of 304 residues and MW of 33 kDa. A concentration of 1.0 µg/mL is recommended to detect MBP in paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Schwartz, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12440382">Prog Brain Res 137, 401-6 (2002) Hauben, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/11518733">J Clin Invest 108, 591-9 (Aug, 2001) Yoles, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11356861">J Neurosci 21, 3740-8 (Jun 1, 2001) Hauben, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10964948">J Neurosci 20, 6421-30 (Sep 1, 2000) Harauz, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15219899">Nature 389, 783-4 (1997). Micron 35, 503-42 (2004) Givogri, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10650873">J Neurosci Res 59, 153-9 (Jan 15, 2000) Kim, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9251242">Int J Biochem Cell Biol 29, 743-51 (May, 1997) Kalwy, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7522797">Mol Membr Biol 11, 67-78 (Apr-Jun, 1994) Wajgt, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6198864">Acta Neurol Scand 68, 337-43 (Nov, 1983) Day, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2418054">J Neuroimmunol 10, 289-312 (Feb, 1986) 1Mikoshiba, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1709079">Comp Biochem Physiol C 98, 51-61 (1991) 1Brophy, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7509522">Trends Neurosci 16, 515-21 (Dec, 1993) 1Matsuo, A. et al. <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1858181/">(1997) Am. J. Pathol. 150(4): 1253-1266
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; expected to react with mouse due to sequence homology
Mouse anti-Calponin-1 Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Background Info:
Calponin is a thin filament associated protein that binds calmodulin, actin and tropomyosin. The multi-gene family of calponins includes calponin 1 (CNN1), 2 (CNN2) and 3 (CNN3). CNN1 is expressed in smooth muscle and tissues containing significant amounts of smooth muscle. This antibody is predicted to detect all three forms of calponin.
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.25-0.50 µg/mL is recommended for WB. Human calponin has a predicted length of 297 aa and a MW of 34 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect calponin in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CNN1; Calponin-1; CNN2; CNN3;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; chicken;
Mouse anti-Microtubule-associated protein tau (MAPT) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Background Info:
FUNCTION: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. SUBUNIT: Interacts with PSMC2 through Sequestosome 1 (SQSTM1). Interacts with SQSTM1 when polyubiquitinated. SUBCELLULAR LOCATION: Cytoplasm; cytosol. Cell membrane. Note=Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components. ALTERNATIVE PRODUCTS: 8 named isoforms produced by alternative splicing. Additional isoforms seem to exist. Isoforms differ from each other by the presence or absence of up to 5 of the 15 exons. One of these optional exons contains the additional tau/MAP repeat. TISSUE SPECIFICITY: Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system. DEVELOPMENTAL STAGE: Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain. DOMAIN: The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats.
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. A concentration of 1.0-2.0 µg/mL is recommended to detect Tau in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; MAPT; MTBT1; TAU
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat
Partially purified inhibitor of actin polymerization (IAP) protein from Turkey Gizzard smooth muscle
Applications:
IHC-Frozen,IHC-Paraffin-embedded,WB
Clone number:
SJ-25
Antibody Isotype:
IgG
Application Details:
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-2.0 µg/mL is recommended for WB. Chicken HSP25 has a predicted length of 172 residues and MW of 19 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. A concentration of 1.0-2.0 µg/mL is recommended to detect HSP25 in formalin/acetone fixed frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Heat shock 25 kDa protein 1; HSP25;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human;
Purification:
IgG
Target:
Heat shock protein 25 (HSP-25)
Accession Number:
Q6BDR9
Research Areas:
Antibodies & Conjugates/Biosensis Stem Cells,Antibodies & Conjugates/Biosensis Cancer
Rabbit anti-Somatostatin Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Background Info:
The active forms of Somatostatin are produced by alternate cleavage of the single preproprotein encoded by the Somatostatin gene. This protein is ubiquitously expressed and inhibits the release of many secondary hormones by binding to high-affinity G-protein-coupled Somatostatin receptors.
A synthetic peptide corresponding to a region (1-14 aa) from the N-terminus of human Somatostatin.
Applications:
IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB) and Immunohistochemistry (IHC). A concentration of 1.0 µg/mL is recommended for WB. Human Somatostatin (precursor) has a predicted length of 116 amino acids and MW of 13 kDa. A concentration of 1.0 µg/mL is recommended for IHC to detect the protein in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. A concentration of 1.0 µg/mL is also recommended for IHC in frozen tissue. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A synthetic peptide corresponding to a region (125-141 aa) from human L-selectin.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Rat L-selectin (precursor) has a predicted length of 372 amino acids and MW of 42 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
