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Product Search Results: r3771

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Product Search Results: r3771

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Biosensis R-3771-100 100 µg £288.00
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Product Detail: Rabbit anti-Neuronal nuclei antigen/Fox3 (NeuN/Fox3) Polyclonal Antibody (Unconjugated), suitable for WB, IHC, ICC.
Background Info: Fox3 is one of a family of mammalian homologues of Fox-1. The Fox proteins are about 46 kDa in size, and each includes a central highly conserved RRM type RNA recognition motif. Much interest has focused on Fox3 as a result of the recent finding that this protein corresponds to NeuN, a neuronal nuclear antigen. NeuN/Fox-3 has a function in RNA splicing and is expressed heavily and specifically in neuronal nuclei and cytoplasm. Our antibody was raised against the N-terminal 100 amino acids of human Fox3 as expressed in and purified from E. coli. We did not use full length Fox3 as immunogen since the three mammalian Fox homologues, namely Fox1, Fox2 and Fox3, include virtually identical RRM motifs. The N-terminal region of the three molecules are much more variable in the three molecules so antibodies specific for each of the three molecules can therefore be generated.
Product Type: Antibody
Antibody Type: Polyclonal
Format: Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal: Rabbit
Species Reactivity: Human,Mouse,Rat
Immunogen: Peptide corresponding to amino acids 5-24 of human FOX3 coupled to KLH.
Applications: ICC,IHC,WB
Antibody Isotype: IgG
Application Details: Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:500 - 1:1,000 is recommended for WB. A dilution of 1:5,000 - 1:10,000 is recommended for ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names: Feminizing locus on X; Fox-1; Fox3; NeuN; NeuN antigen; Neuronal nuclei antigen; Fox-1 homolog C; RNA binding protein fox-1 homolog 3
Biosensis Brand: Biosensis®
Conjugate: Unconjugated
Shelf Life: 12 months after date of receipt (unopened vial).
Use: For research use only.
Storage: Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.
Purification: Affinity purified
Target: Neuronal nuclei antigen/Fox3 (NeuN/Fox3)
Uniprot Number: A6NFN3