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Product Search Results: R-1751-50

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Product Search Results: R-1751-50

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Biosensis R-1751-50 50 µg £288.00
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Product Detail: Rabbit anti-Cellular oncogene fos (c-FOS) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, ICC, WB.
Background Info: FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB. SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily. SIMILARITY: Contains 1 bZIP domain (Ref: uniprot.org).
Product Type: Antibody
Antibody Type: Polyclonal
Format: Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Host Animal: Rabbit
Species Reactivity: Human,Mouse,Rat
Immunogen: Full length, E.coli-derived recombinant human c-FOS protein.
Applications: ICC,IHC-Frozen,WB
Antibody Isotype: IgG
Application Details: Immunocytochemistry (ICC): 1:2,000-1:10,000.<br><br>Immunohistochemistry (IHC): 1:20,000-1:50,000. This antibody has been shown to work on 4% PFA fixed mouse brain sections. Note that non-specific staining has been observed on tissue sections when using this antibody at dilutions of 1:5,000 or lower.<a class="newA" target="_blank" href="https://www.biosensis.com/documents/enhancedinfo/R-1751-50_IHC Method_as_at_March2018.pdf"> Click here </a> for instructions on use of this antibody in IHC on free-floating brain sections.<br><br>Western blotting (WB): 1:1,000-1:2,000. This antibody detects bands between 50-65 kDa, which only appear in stimulated cells. <br><br>Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names: Cellular oncogene fos; G0/G1 switch regulatory protein 7; cFOS
Biosensis Brand: Biosensis®
Conjugate: Unconjugated
Shelf Life: 12 months after date of receipt (unopened vial).
Use: For research use only.
Product references: Lin King JV (2020). Stinging Sensations: Activation Mechanisms of the Wasabi Receptor, TRPA1. PhD Thesis. Application: IHC (IF) . Species: Mouse. Lin King JV et al. (2019). A Cell-Penetrating Scorpion Toxin Enables Mode-Specific Modulation of TRPA1 and Pain. Cell. [Epub ahead of print]. Application: IHC . Species: Mouse.
Storage: Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Purification: Affinity purified.
Target: Cellular oncogene fos (c-FOS)
Uniprot Number: P01100