A ready-to-use 5X-concentrated solution for High Resolution Melt (HRM) analysis of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection. The mix has two versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
excellent resolution in HRM assays allows detection of DNA sequence variations contains sensitive EvaGreen® dye compatible with most cyclers different product versions: no ROX, ROX reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye ROX reference dye Bovine serum albumin (BSA) to enhance qPCR reaction
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye
Sensitive and highly specific cDNA synthesis and probe-based qPCR in a single tube. Ideal solution for high throughput RNA analysis.
Background Info:
A convenient kit for analysis of RNA targets and performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit is optimised for sensitive quantification of up to 4 targets in one reaction using TaqMan® or other hydrolysis probe types with enhanced amplification of GC-rich targets. It contains RiboGrip RNase inhibitor and dUTPs to prevent cross-contamination when used with UNG treatment. It has three versions to match different instruments and assay requirements. ROX-kit is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-kit is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
RT-qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cDNA synthesis up to 60°C for superior specificity analyze 1-4 targets in 1 reaction robust amplification of GC-rich targets contains dUTP to prevent cross-contamination when used in combination with UNG RNase inhibitor included in kit wide instrument compatibility reaction set-up and shipment without ice
Mix/Kit Components:
5x One-step Multiplex Probe Mix with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2) 40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase, RiboGrip RNase Inhibitor Water, nuclease free
Highly specific and reproducible dye-based qPCR Mix with blue visualisation dye for easy pipetting. Suitable for ROX-dependent and ROX-independent qPCR cyclers.
Background Info:
A universal ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix is optimised for highly specific results and reduced primer-dimer formation with excellent amplification of GC-rich regions. The Mix contains dUTPs to prevent cross-contamination when used with UNG treatment, and a visible dye to ease reaction set-up. It contains an internal reference based on ROX dye and is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
high sensitivity with low DNA concentrations robust amplification of GC-rich targets blue visualization dye to ease pipetting reduced primer dimer formation contains dUTP to prevent cross-contamination when used in combination with UNG one qPCR mix for all cyclers (except capillary) reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs, including dUTP: mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® EvaGreen® Supermix EvaGreen® dye Internal reference based on ROX dye GC-enhancer Blue visualisation dye
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12.5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Bovine serum albumine (BSA) to enhance qPCR reaction
Ultra-fast endpoint PCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target detection. Includes two tracking dyes.
Background Info:
A ready-to-use 5X-concentrated solution for fast and ultra-fast singleplex and multiplex endpoint PCR assays. It contains a novel SolisFAST® DNA Polymerase with fast hot-start (30 sec to 3 min) and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases. The mix allows amplification of up to 5 kb DNA templates and has two versions - regular and Ready-to-Load mix which includes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
PCR results in 20 minutes fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) sensitive detection of up to 18 targets per reaction suitable for templates up to 5 kb reliable results in Sanger sequencing applications Ready-to-Load mix available for direct loading to gel UNG-mix available to prevent carry-over contamination reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase. PCR buffer: Includes 17.5 mM MgCl2 (1x PCR solution 3.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), additives that maximize efficiency of PCR Green loading dye: Mix of blue and yellow dye to allow tracking of DNA during electrophoresis. The mix is not recommended for use in applications where spectrophotometric measurements are necessary (e.g. Sanger sequencing). Migration speed: blue dye TBE: ~3.5 kb, TAE: ~4.5 kb; yellow dye TBE: ~35 bp, TAE: ~45 bp).
Sensitive and highly specific cDNA synthesis and probe-based qPCR in a single tube. Ideal solution for high throughput RNA analysis.
Background Info:
A convenient kit for analysis of RNA targets and performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit is optimised for sensitive quantification of up to 4 targets in one reaction using TaqMan® or other hydrolysis probe types with enhanced amplification of GC-rich targets. It contains RiboGrip RNase inhibitor and dUTPs to prevent cross-contamination when used with UNG treatment. It has three versions to match different instruments and assay requirements. ROX-kit is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-kit is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
RT-qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cDNA synthesis up to 60°C for superior specificity analyze 1-4 targets in 1 reaction robust amplification of GC-rich targets contains dUTP to prevent cross-contamination when used in combination with UNG RNase inhibitor included in kit wide instrument compatibility reaction set-up and shipment without ice
Mix/Kit Components:
5x One-step Multiplex Probe Mix (ROX) with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2), ROX dye (If ROX dye is used as one of the fluorophores, internal passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available) 40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase and RiboGrip RNase Inhibitor Water, nuclease-free
An optimized formulation for SARS-CoV-2 RNA detection from RNA samples extracted from swab specimens. The Kit enables the users to develop their own assays based on US CDC and WHO-listed primer and probe sequences to detect SARS-CoV-2, as well as develop custom multi-target tests.
Background Info:
A convenient and sensitive kit for analysis of RNA targets and performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit is optimised for detection of SARS-CoV-2 RNA and it enables the users to develop their own assays based on US CDC and WHO-listed primer and probe sequences, as well as develop custom multi-target tests. The kit contains RiboGrip RNase inhibitor and is compatible with most cyclers if signal normalisation is not desired. A kit containg ROX passive reference is available upon request.
Product Type:
RT-qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
optimized for SARS-CoV-2 detection analyze 1-4 targets in 1 reaction RNase inhibitor included in kit wide instrument compatibility reaction set-up and shipment without ice
Mix/Kit Components:
5x One-step Probe CoV Mix with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2) 40x One-step SOLIScript® CoV Mix with SOLIScript® Reverse Transcriptase and RiboGrip RNase Inhibitor Water, nuclease free
Highly sensitive dye-based qPCR Mix with improved detection of low-copy targets. Compatible with most qPCR platforms.
Background Info:
A highly sensitive ready-to-use 5X reaction mix for dye-based qPCR detection of DNA targets using SolisGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has excellent performance with low template amounts. It contains contains an internal reference based on ROX dye and is compatible with qPCR instruments that require low ROX level for signal normalization. SolisGreen® qPCR mixes are suitable for commercial and diagnostic applications and require no additional licensing.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
enhanced performance with low DNA concentrations bright and sensitive SolisGreen® dye suitable for commercial applications one qPCR mix for all cyclers, except high ROX reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x qPCR buffer with 12.5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP SolisGreen® dye Bovine serum albumin (BSA) to enhance PCR reaction Internal reference based on ROX dye
Sensitive and highly specific cDNA synthesis and probe-based qPCR in a single tube. Ideal solution for high throughput RNA analysis.
Background Info:
A convenient kit for analysis of RNA targets and performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit is optimised for sensitive quantification of up to 4 targets in one reaction using TaqMan® or other hydrolysis probe types with enhanced amplification of GC-rich targets. It contains RiboGrip RNase inhibitor and dUTPs to prevent cross-contamination when used with UNG treatment. It has three versions to match different instruments and assay requirements. ROX-kit is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-kit is compatible with instruments that use Mustang Purple for signal normalisation.
Product Type:
RT-qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cDNA synthesis up to 60°C for superior specificity analyze 1-4 targets in 1 reaction robust amplification of GC-rich targets contains dUTP to prevent cross-contamination when used in combination with UNG RNase inhibitor included in kit wide instrument compatibility reaction set-up and shipment without ice
Mix/Kit Components:
5x One-step Multiplex Probe Mix (Purple) with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2), Purple dye (If Cy5 dye is used as one of the fluorophores, internal passive reference dye might interfere with the signal. A version without passive reference or with ROX reference dye is available) 40x One-step SOLIScript® Mix with SOLIScript® Reverse and Transcriptase and RiboGrip RNase Inhibitor Water, nuclease free
Highly sensitive cDNA synthesis and dye-based qPCR in a single tube. Ideal solution for high throughput RNA analysis.
Background Info:
A convenient and sensitive kit for analysis of RNA targets and performing cDNA synthesis and a dye-based qPCR in a single tube using SolisGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The Kit includes 3 components: a 40x One-step SOLIScript® Mix for cDNA synthesis, a 5x One-step SolisGreen® Mix for dye-based qPCR, and nuclease-free water. The 5x One-step SolisGreen® Mix contains an internal reference based on ROX dye and is compatible with most qPCR instruments, except those that require high ROX level for signal normalization.
Product Type:
RT-qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cDNA cynthesis and dye-based qPCR in one tube high specificity, sensitivity and yield consistent results with low RNA input RNase inhibitor included in kit reaction set-up and shipment without ice
A ready-to-use 5X-concentrated solution for High Resolution Melt (HRM) analysis of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection. The mix has two versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
excellent resolution in HRM assays allows detection of DNA sequence variations contains sensitive EvaGreen® dye compatible with most cyclers different product versions: no ROX, ROX reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2, 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Bovine serum albumin (BSA) to enhance qPCR reaction
Ultra fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification. Suitable for UNG treatment to prevent cross-contamination.
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) analyze 1-5 targets in 1 reaction mix with dUTP and UNG available to prevent carry-over contamination compatible with most cyclers different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start, and faster extension rates compared to the wild-type Taq DNA polymerase.
qPCR buffer: Includes 16.5 mM MgCl2 (1x PCR solution 3.3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR
Ultra fast endpoint PCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target detection. Suitable for UNG treatment to prevent cross-contamination. Includes two tracking dyes.
Background Info:
A ready-to-use 5X-concentrated solution for fast and ultra-fast singleplex and multiplex endpoint PCR assays. It contains a novel SolisFAST® DNA Polymerase with fast hot-start (30 sec to 3 min) and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases. The mix allows amplification of up to 5 kb DNA templates and has two versions - regular and Ready-to-Load mix which includes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
PCR results in 20 minutes fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) sensitive detection of up to 18 targets per reaction suitable for templates up to 5 kb reliable results in Sanger sequencing applications Ready-to-Load mix available for direct loading to gel UNG-mix available to prevent carry-over contamination reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase. PCR buffer: Includes 17.5 mM MgCl2 (1x PCR solution 3.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR Green loading dye: Mix of blue and yellow dye to allow tracking of DNA during electrophoresis. The mix is not recommended for use in applications where spectrophotometric measurements are necessary (e.g. Sanger sequencing). Migration speed: blue dye TBE: ~3.5 kb, TAE: ~4.5 kb; yellow dye TBE: ~35 bp, TAE: ~45 bp).
Extremely fast and highly sensitive dye-based qPCR that includes novel in-silico designed SolisFAST® DNA Polymerase Mix for most demanding applications
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using SolisGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases. It has two versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low ROX level for signal normalization. SolisGreen® qPCR mixes are suitable for commercial and diagnostic applications and require no additional licensing.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) bright and sensitive SolisGreen® dye suitable for commercial applications compatible with most cyclers, except high ROX different product versions: no ROX and ROX reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.
qPCR buffer: Includes 7.5 mM MgCl2 (1X PCR solution 1.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), stabilizers and enhancers that maximize efficiency of PCR reaction
Reference dye: ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR
Fluorescent dye: SolisGreen® is a nucleic acid dye detected in the FAM or SYBR® Green I channel
Ultra-fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification.
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) analyze 1-5 targets in 1 reaction mix with dUTP and UNG available to prevent carry-over contamination compatible with most cyclers different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start, and faster extension rates compared to the wild-type Taq DNA polymerase. qPCR buffer: Includes 15 mM MgCl2 (1x PCR solution 3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), additives that maximize efficiency of PCR Reference dye: ROX dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR
Ultra fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification. Suitable for UNG treatment to prevent cross-contamination.
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) analyze 1-5 targets in 1 reaction mix with dUTP and UNG available to prevent carry-over contamination compatible with most cyclers different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase. qPCR buffer: Includes 16.5 mM MgCl2 (1x PCR solution 3.3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR
Ultra-fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification.
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) analyze 1-5 targets in 1 reaction mix with dUTP and UNG available to prevent carry-over contamination compatible with most cyclers different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase. qPCR buffer: Includes 15 mM MgCl2 (1x PCR solution 3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), additives that maximize efficiency of PCR
Ultra fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification. Suitable for UNG treatment to prevent cross-contamination.
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) analyze 1-5 targets in 1 reaction mix with dUTP and UNG available to prevent carry-over contamination compatible with most cyclers different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start, and faster extension rates compared to the wild-type Taq DNA polymerase. qPCR buffer: Includes 16.5 mM MgCl2 - 1x PCR solution 3.3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG and additives that maximize efficiency of PCR Reference dye: Purple dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR
Sensitive and highly specific cDNA synthesis and qPCR in a single tube. Suitable for ROX and no ROX cyclers.
Background Info:
A convenient kit for analysis of RNA targets and performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit is optimised for sensitive detection of up to 2 targets in one reaction using TaqMan® or other hydrolysis probe types. It contains RiboGrip RNase inhibitor and an internal reference based on ROX dye and is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization. The kit is not compatible with Probes detected in ROX/JUN/Texas Red channel.
Product Type:
RT-qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cDNA synthesis up to 60°C for superior specificity suitable for assays with 1-2 targets high specificity and sensitivity RNase inhibitor included in kit one kit for all cyclers reaction set-up and shipment without ice
Mix/Kit Components:
5x One-step Multiplex Probe Mix (ROX) with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2), internal reference based on ROX dye 40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase and RiboGrip RNase Inhibitor Water, nuclease free
Ultra-fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification.
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.
Product Type:
Probe-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) analyze 1-5 targets in 1 reaction mix with dUTP and UNG available to prevent carry-over contamination compatible with most cyclers different product versions: no ROX, ROX, Purple reaction set-up and shipment without ice
Ultra-fast endpoint PCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target detection. Suitable for UNG treatment to prevent cross-contaminaton
Background Info:
A ready-to-use 5X-concentrated solution for fast and ultra-fast singleplex and multiplex endpoint PCR assays. It contains a novel SolisFAST® DNA Polymerase with fast hot-start (30 sec to 3 min) and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases. The mix allows amplification of up to 5 kb DNA templates and has two versions - regular and Ready-to-Load mix which includes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
PCR results in 20 minutes fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) sensitive detection of up to 18 targets per reaction suitable for templates up to 5 kb reliable results in Sanger sequencing applications Ready-to-Load mix available for direct loading to gel UNG-mix available to prevent carry-over contamination reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase PCR Buffer: Includes 17.5 mM MgCl2 (1x PCR solution 3.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR
Extremely fast and highly sensitive dye-based qPCR that includes novel in-silico designed SolisFAST® DNA Polymerase Mix for most demanding applications
Background Info:
Fast and sensitive ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using SolisGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases. It has two versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low ROX level for signal normalization. SolisGreen® qPCR mixes are suitable for commercial and diagnostic applications and require no additional licensing.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
qPCR results 2x faster fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) bright and sensitive SolisGreen® dye suitable for commercial applications compatible with most cyclers, except high ROX different product versions: no ROX and ROX reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico- designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.
qPCR buffer: Includes 7.5 mM MgCl2 (1X PCR solution 1.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), stabilizers and enhancers that maximize efficiency of PCR reaction
Fluorescent dye: SolisGreen® is a nucleic acid dye detected in the FAM or SYBR® Green I channel
Ultra-fast endpoint PCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target detection.
Background Info:
A ready-to-use 5X-concentrated solution for fast and ultra-fast singleplex and multiplex endpoint PCR assays. It contains a novel SolisFAST® DNA Polymerase with fast hot-start (30 sec to 3 min) and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases. The mix allows amplification of up to 5 kb DNA templates and has two versions - regular and Ready-to-Load mix which includes loading dyes for direct loading to gel.
Product Type:
Endpoint PCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
PCR results in 20 minutes fast hot-start (30 sec-3 min) fast amplification (15-30 sec/kb) sensitive detection of up to 18 targets per reaction suitable for templates up to 5 kb reliable results in Sanger sequencing applications Ready-to-Load mix available for direct loading to gel UNG-mix available to prevent carry-over contamination reaction set-up and shipment without ice
Mix/Kit Components:
SolisFAST® DNA Polymerase: In silico designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase. PCR buffer: Includes 17.5 mM MgCl2 (1x PCR solution 3.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP) and additives that maximize efficiency of PCR
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
Background Info:
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.
Product Type:
Dye-based qPCR
Format:
Master Mix
Storage Temp:
Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of this product.
cost-effective solution for less demanding assays suitable for most dye-based applications high sensitivity and specificity excellent efficiency compatible with most cyclers different product versions: no ROX, ROX, capillary reaction set-up and shipment without ice
Mix/Kit Components:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution 2.5 mM MgCl2 dNTPs: dATP, dCTP, dGTP and dTTP EvaGreen® dye Internal reference based on ROX dye
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