Bioo Scientifics NEXTflex Rapid DNA-Seq Kit is a rapid DNA library prep kit producing libraries for Illumina® sequencing from as little as 1 ng of DNA in two hours. This kit is ideal for use with genomic DNA, FFPE samples, ChIP DNA, upstream target capture samples, pooled amplicons and low input clinical samples. This DNA-Seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of input DNA amounts from 1 ng to 1 µg. The optimized protocol allows for fast generation of non-size-selected libraries and size-selected libraries using reproducible gel-free double SPRI selection.
The NEXTflex Rapid DNA-Seq Kit incorporates Enhanced Adapter Ligation Technology, which facilitates ligation of long adapters, resulting in longer and more diverse sequencing reads. Bioo Scientifics NEXTflex Ligation and Polymerase reaction mixes ensure the highest quality libraries for superior performance. Study results in the white paper, "Optimizing Library Preparation: Enhanced Adapter Ligation Technology", demonstrate the superior ligation efficiency of the NEXTflex Rapid DNA-Seq Library Prep Kit for creating NGS libraries for Illumina® sequencing.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Flexible amounts of input DNA from 1 ng to 1 µg Fast workflow requiring 2 hours or less, with minimal hands-on time Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads Creates libraries with less bias than alternative methods Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Kits containing up to 96 unique barcodes Compatible with Illumina HiSeq, MiSeq and GAIIx platforms Prepare single, paired-end and multiplexed genomic DNA libraries Bulk pricing available to meet research goals
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex Adapters are long, annealed adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. These barcodes can be used with single, paired-end and multiplex reads. Bioo Scientific strives to make available flexible barcoding options. The NEXTflex Rapid DNA-Seq Kit is designed to be used with the NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes and NEXTflex-HT Barcodes when 10 ng or more of starting material is used. These barcodes are available in sets of 6, 12, 24, 48, 96 and 384 unique indexed adapters.
When less than 10 ng of starting material is available, the NEXTflex ChIP-Seq Barcodes and NEXTflex-96 ChIP-Seq Barcodes should be used. These barcodes are available in sets of 6, 12, 24, 48, and 96 unique indexed adapters.
The NEXTflex Rapid DNA-Seq Kit contains enough material to prepare 8 or 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
The NEXTflex-HT Barcodes are designed to prepare High Throughput (HT) multiplexed, single or paired-end libraries for sequencing using Illumina platforms. The index and flow cell binding sequence are contained within the NEXTflex-HT Barcodes and are attached onto sample inserts during adapter ligation. NEXTflex-HT Barcodes allow for at least two error corrections and have multiple low-diversity pooling options.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - High Throughput
Applications:
Rapid DNA
Search Terms:
HT Barcodes
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514170
Bioo Scientifics NEXTflex Rapid DNA-Seq Kit is a rapid DNA library prep kit producing libraries for Illumina® sequencing from as little as 1 ng of DNA in two hours. This kit is ideal for use with genomic DNA, FFPE samples, ChIP DNA, upstream target capture samples, pooled amplicons and low input clinical samples. This DNA-Seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of input DNA amounts from 1 ng to 1 µg. The optimized protocol allows for fast generation of non-size-selected libraries and size-selected libraries using reproducible gel-free double SPRI selection.
The NEXTflex Rapid DNA-Seq Kit incorporates Enhanced Adapter Ligation Technology, which facilitates ligation of long adapters, resulting in longer and more diverse sequencing reads. Bioo Scientifics NEXTflex Ligation and Polymerase reaction mixes ensure the highest quality libraries for superior performance. Study results in the white paper, "Optimizing Library Preparation: Enhanced Adapter Ligation Technology", demonstrate the superior ligation efficiency of the NEXTflex Rapid DNA-Seq Library Prep Kit for creating NGS libraries for Illumina® sequencing.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Flexible amounts of input DNA from 1 ng to 1 µg Fast workflow requiring 2 hours or less, with minimal hands-on time Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads Creates libraries with less bias than alternative methods Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Kits containing up to 96 unique barcodes Compatible with Illumina HiSeq, MiSeq and GAIIx platforms Prepare single, paired-end and multiplexed genomic DNA libraries Bulk pricing available to meet research goals
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex Adapters are long, annealed adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. These barcodes can be used with single, paired-end and multiplex reads. Bioo Scientific strives to make available flexible barcoding options. The NEXTflex Rapid DNA-Seq Kit is designed to be used with the NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes and NEXTflex-HT Barcodes when 10 ng or more of starting material is used. These barcodes are available in sets of 6, 12, 24, 48, 96 and 384 unique indexed adapters.
When less than 10 ng of starting material is available, the NEXTflex ChIP-Seq Barcodes and NEXTflex-96 ChIP-Seq Barcodes should be used. These barcodes are available in sets of 6, 12, 24, 48, and 96 unique indexed adapters.
The NEXTflex Rapid DNA-Seq Kit contains enough material to prepare 8 or 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
The NEXTflex-HT Barcodes are designed to prepare High Throughput (HT) multiplexed, single or paired-end libraries for sequencing using Illumina platforms. The index and flow cell binding sequence are contained within the NEXTflex-HT Barcodes and are attached onto sample inserts during adapter ligation. NEXTflex-HT Barcodes allow for at least two error corrections and have multiple low-diversity pooling options.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - High Throughput
Applications:
Rapid DNA
Search Terms:
HT Barcodes
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514174
The NEXTflex-HT Barcodes are designed to prepare High Throughput (HT) multiplexed, single or paired-end libraries for sequencing using Illumina platforms. The index and flow cell binding sequence are contained within the NEXTflex-HT Barcodes and are attached onto sample inserts during adapter ligation. NEXTflex-HT Barcodes allow for at least two error corrections and have multiple low-diversity pooling options.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - High Throughput
Applications:
Rapid DNA
Search Terms:
HT Barcodes
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514175
The NEXTflex-HT Barcodes are designed to prepare High Throughput (HT) multiplexed, single or paired-end libraries for sequencing using Illumina platforms. The index and flow cell binding sequence are contained within the NEXTflex-HT Barcodes and are attached onto sample inserts during adapter ligation. NEXTflex-HT Barcodes allow for at least two error corrections and have multiple low-diversity pooling options.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - High Throughput
Applications:
Rapid DNA
Search Terms:
HT Barcodes
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514176
The NEXTflex-HT Barcodes are designed to prepare High Throughput (HT) multiplexed, single or paired-end libraries for sequencing using Illumina platforms. The index and flow cell binding sequence are contained within the NEXTflex-HT Barcodes and are attached onto sample inserts during adapter ligation. NEXTflex-HT Barcodes allow for at least two error corrections and have multiple low-diversity pooling options.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - DNA - High Throughput
Applications:
Rapid DNA
Search Terms:
HT Barcodes
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 514177
Bioo Scientifics NEXTflex Rapid DNA-Seq Kit is a rapid DNA library prep kit producing libraries for Illumina® sequencing from as little as 1 ng of DNA in two hours. This kit is ideal for use with genomic DNA, FFPE samples, ChIP DNA, upstream target capture samples, pooled amplicons and low input clinical samples. This DNA-Seq library prep kit is highly flexible in terms of sample requirements, accommodating a range of input DNA amounts from 1 ng to 1 µg. The optimized protocol allows for fast generation of non-size-selected libraries and size-selected libraries using reproducible gel-free double SPRI selection.
The NEXTflex Rapid DNA-Seq Kit incorporates Enhanced Adapter Ligation Technology, which facilitates ligation of long adapters, resulting in longer and more diverse sequencing reads. Bioo Scientifics NEXTflex Ligation and Polymerase reaction mixes ensure the highest quality libraries for superior performance. Study results in the white paper, "Optimizing Library Preparation: Enhanced Adapter Ligation Technology", demonstrate the superior ligation efficiency of the NEXTflex Rapid DNA-Seq Library Prep Kit for creating NGS libraries for Illumina® sequencing.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Flexible amounts of input DNA from 1 ng to 1 µg Fast workflow requiring 2 hours or less, with minimal hands-on time Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads Creates libraries with less bias than alternative methods Automation-friendly workflow is compatible with liquid handlers Flexible barcode options Kits containing up to 96 unique barcodes Compatible with Illumina HiSeq, MiSeq and GAIIx platforms Prepare single, paired-end and multiplexed genomic DNA libraries Bulk pricing available to meet research goals
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
The NEXTflex Adapters are long, annealed adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. These barcodes can be used with single, paired-end and multiplex reads. Bioo Scientific strives to make available flexible barcoding options. The NEXTflex Rapid DNA-Seq Kit is designed to be used with the NEXTflex DNA Barcodes, NEXTflex-96 DNA Barcodes and NEXTflex-HT Barcodes when 10 ng or more of starting material is used. These barcodes are available in sets of 6, 12, 24, 48, 96 and 384 unique indexed adapters.
When less than 10 ng of starting material is available, the NEXTflex ChIP-Seq Barcodes and NEXTflex-96 ChIP-Seq Barcodes should be used. These barcodes are available in sets of 6, 12, 24, 48, and 96 unique indexed adapters.
The NEXTflex Rapid DNA-Seq Kit contains enough material to prepare 8 or 48 DNA samples for Illumina compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C.
These kits ship on dry ice.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
NGS Applications:
Rapid DNA-Sequncing
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