FUNCTION: Seems to promote the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Storage Temp:
Short term storage at 2-8 °C for one week. At -20 °C as an undiluted liquid for up to 12 months.
Host Animal:
Chicken
Species Reactivity:
Human, Mouse
Immunogen:
Mixture of two human NT3 peptides (90-104 and 199-214 aa).
Try our new NT3 Rapid ELISA kit for Human, Rat and Mouse (BEK-2221-1P). Same specificity and sensitivity, but faster!<The human NT3 Kit is a sandwich ELISA. The capture antibody is a monoclonal anti-NT3 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known NT3 concentration are added to these wells and allowed to complex with the bound NT3 antibody. A biotinylated NT3 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of NT3 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human NT3 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human, Mouse, Rat
Immunogen:
The standard and immunogen in this assays is produced in and purified from an insect expression system consisting of amino acids Y139-T257
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Human; Mouse; Rat; | Less than 2.5% cross reactivity with NT-4. No detectable cross-reactivity with any other cytokines.
Try our new NT3 Rapid ELISA kit for Human, Rat and Mouse (BEK-2221-2P). Same specificity and sensitivity, but faster!The human NT3 Kit is a sandwich ELISA. The capture antibody is a monoclonal anti-NT3 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known NT3 concentration are added to these wells and allowed to complex with the bound NT3 antibody. A biotinylated NT3 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of NT3 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human NT3 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human, Mouse, Rat
Immunogen:
The standard and immunogen in this assays is produced in and purified from an insect expression system consisting of amino acids Y139-T257
The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Human; Mouse; Rat; | Less than 2.5% cross reactivity with NT-4. No detectable cross-reactivity with any other cytokines.
Try our new NT3 Rapid ELISA kit for Human, Rat and Mouse (BEK-2221-1P). Same specificity and sensitivity, but faster!<The mouse NT3 Kit is a sandwich ELISA. The capture antibody is a monoclonal NT3 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known NT3 concentration are added to these wells and allowed to complex with the bound NT3 antibody. A biotinylated NT3 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of NT3 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse NT3 in samples such as sera and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human, Mouse, Rat
Immunogen:
The standard and immunogen in this assays is produced in and purified from an insect expression system consisting of amino acids Y139-T257
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Human; Mouse; Rat; | Less than 2.5% cross reactivity with NT-4. No detectable reactivity with other relevant proteins.
Assay Range:
15.6 pg/mL - 1000 pg/mL
Sensitivity:
< 2 pg/mL
References:
Davide D'Amico, Thomas Gener, Maria MartÃnez de Lagrán, Maria V Sanchez-Vives, Mónica Santos, Mara Dierssen (2016) Infralimbic Neurotrophin-3 Infusion Rescues Fear Extinction Impairment in a Mouse Model of Pathological Fear. Neuropsychopharmacology. [Epub ahead of print] doi:10.1016/j.neulet.2015.12.016.Sandra Petzold, Babette Sommer, Andrea Krober, Robert Nitsch, Herbert Schwegler, Johannes Vogt, Thomas Roskoden (2015) NT-3 protein levels are enhanced in the hippocampus of PRG1-deficient mice but remain unchanged in PRG1/LPA2 double mutants. Neuroscience Letters. 612:145-8 doi:10.1016/j.neulet.2015.12.016.
Try our new NT3 Rapid ELISA kit for Human, Rat and Mouse (BEK-2221-2P). Same specificity and sensitivity, but faster!The mouse NT3 Kit is a sandwich ELISA. The capture antibody is a monoclonal NT3 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known NT3 concentration are added to these wells and allowed to complex with the bound NT3 antibody. A biotinylated NT3 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of NT3 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse NT3 in samples such as sera and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human, Mouse, Rat
Immunogen:
The standard and immunogen in this assays is produced in and purified from an insect expression system consisting of amino acids Y139-T257
The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Mouse NT3 ELISA Kit
Assay Range:
15.6 pg/mL - 1000 pg/mL
Sensitivity:
< 2 pg/mL
References:
Davide D'Amico, Thomas Gener, Maria MartÃnez de Lagrán, Maria V Sanchez-Vives, Mónica Santos, Mara Dierssen (2016) Infralimbic Neurotrophin-3 Infusion Rescues Fear Extinction Impairment in a Mouse Model of Pathological Fear. Neuropsychopharmacology. [Epub ahead of print] doi:10.1016/j.neulet.2015.12.016.Sandra Petzold, Babette Sommer, Andrea Krober, Robert Nitsch, Herbert Schwegler, Johannes Vogt, Thomas Roskoden (2015) NT-3 protein levels are enhanced in the hippocampus of PRG1-deficient mice but remain unchanged in PRG1/LPA2 double mutants. Neuroscience Letters. 612:145-8 doi:10.1016/j.neulet.2015.12.016.
NT3 is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It seems to promote the survival of visceral and proprioceptive sensory neurons. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. NT3 is a secreted protein that is expressed in brain and peripheral tissues.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. 50% glycerol
Storage Temp:
Keep aliquots at -20 ºC for higher stability or at 4 ºC with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial recombinant human NT3 (19-128) with a GST tag.
The Biosensis NT3 RapidTM enzyme-linked immune-sorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT3 in less than 4 hours in cell culture supernatants and human plasma (EDTA and citrate) only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human plasma. Accurate quantification of NT3 in human plasma requires a heterophilic antibody (HA) blocker which can be purchased separately (BL-004-500).This ELISA kit consists of a pre-coated polyclonal anti-NT3 capture antibody, a biotinylated monoclonal anti-NT3 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product, which is directly proportional to the concentration of NT3 present in samples and protein standards. This NT3 RapidTM ELISA kit employs a recombinant human NT3 standard. NT3 is highly conserved and nearly identical in many species. The assay's capture and detection antibodies detect NT3 from other species including mouse, rat and monkey, thus it is expected that this NT3 RapidELISA will also react with those species and possibly more because of the conversation of the immunogen used. The antibodies used in this kit bind to epitopes within the mature domain of NT3. Thus, this ELISA kit may detect the full-length pro-form of NT3. This kit has not been tested for other applications. Sufficient amount of NT3 standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
NT3 is understood to be important in promote the survival of visceral and proprioceptive sensory neurons. It is a secreted protein that belongs to the NGF-beta family and is found in brain and peripheral tissues.
Product Type:
ELISA
Antibody Type:
Monoclonal-Monoclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Shelf Life:
12 months from purchase.
Specificity:
Human. The capture and detection antibodies used detect NT3 from other species including mouse, rat and monkey, thus it is expected that this NT3 RapidELISA will also react with those species. |
Assay Range:
15.6 - 1000 pg/mL
Sensitivity:
This NT3 ELISA kit typically detects a minimum of 5 pg/mL NT3 (defined as 150% of blank value).
References:
Shen W, Yau B, Lee SR, Zhu L, Yam M, Gillies MC (2017) Effects of Ranibizumab and Aflibercept on Human Müller Cells and Photoreceptors under Stress Conditions. Int J Mol Sci. 2017 Mar 1;18(3). pii: E533. doi: 10.3390/ijms18030533. Application: Human cell line supernatants.
Related Products:
BL-004-500, Heterophilic antibody blocker: Sample diluent additive for accurate quantification or NT3 in human plasma (EDTA and citrate).
The Biosensis NT3 RapidTM enzyme-linked immune-sorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT3 in less than 4 hours in cell culture supernatants and human plasma (EDTA and citrate) only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human plasma. Accurate quantification of NT3 in human plasma requires a heterophilic antibody (HA) blocker which can be purchased separately (BL-004-500).This ELISA kit consists of a pre-coated polyclonal anti-NT3 capture antibody, a biotinylated monoclonal anti-NT3 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product, which is directly proportional to the concentration of NT3 present in samples and protein standards. This NT3 RapidTM ELISA kit employs a recombinant human NT3 standard. NT3 is highly conserved and nearly identical in many species. The assay's capture and detection antibodies detect NT3 from other species including mouse, rat and monkey, thus it is expected that this NT3 RapidTM ELISA will also react with those species and possibly more because of the conversation of the immunogen used. The antibodies used in this kit bind to epitopes within the mature domain of NT3. Thus, this ELISA kit may detect the full-length pro-form of NT3. This kit has not been tested for other applications. Sufficient amount of NT3 standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
NT3 is understood to be important in promote the survival of visceral and proprioceptive sensory neurons. It is a secreted protein that belongs to the NGF-beta family and is found in brain and peripheral tissues.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Shelf Life:
12 months from purchase.
Specificity:
Human. The capture and detection antibodies used detect NT3 from other species including mouse, rat and monkey, thus it is expected that this NT3 ELISA will also react with those species. |
Assay Range:
15.6 - 1000 pg/mL
Sensitivity:
This NT3 ELISA kit typically detects a minimum of 5 pg/mL NT3 (defined as 150% of blank value).
References:
Shen W, Yau B, Lee SR, Zhu L, Yam M, Gillies MC (2017) Effects of Ranibizumab and Aflibercept on Human Müller Cells and Photoreceptors under Stress Conditions. Int J Mol Sci. 2017 Mar 1;18(3). pii: E533. doi: 10.3390/ijms18030533. Application: Human cell line supernatants.
Related Products:
BL-004-500, Heterophilic antibody blocker: Sample diluent additive for accurate quantification or NT3 in human plasma (EDTA and citrate).
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display sevvere movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Glycerol (1:) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Rat, Chicken, Human
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human (aa: 139-149), mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Reconstitute in 20 µL of sterile water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. | This antiserum is known to react with rat, chicken and human NT3.
FUNCTION: Seems to promotes the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Host Animal:
Rabbit
Species Reactivity:
Rat, Chicken, Human
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human (aa: 139-149), mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. | This antiserum is known to react with rat, chicken and human NT3.
References:
Feron F et al (2008) Neurotrophin expression in the adult olfactory epithelium. Brain Res. 1196:13-21 Application: IHC; Species: Rat
FUNCTION: Seems to promote the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Glycerol (1:) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Rat, Chicken, Human
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human (aa: 139-149), mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
IHC, ELISA, WB, inhibition of biological activity. A dilution of 1:500 to 1:2000 is recommended for IHC, western blot. For inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. | This antiserum is known to react with rat, chicken and human NT3.
FUNCTION: Seems to promotes the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Glycerol (1:) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
A cross reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by dot blot. | This antiserum is known to react with rat, chicken and human NT3.
FUNCTION: Seems to promotes the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Glycerol (1:) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
IHC, ELISA, WB, inhibition of biological activity. A dilution of 1:500 to 1:1000 is recommended for IHC, ELISA and western blot. For inhibition of biological activity: 1:10-50 for in vitro, 2-10 µL/g body weight for in vivo. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
A cross reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by dot blot. | This antiserum is known to react with rat, chicken and human NT3.
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Maintain lyophilized antibody at 2-8 °C. After reconstitution, aliquot and store at -20 °C for up to 6 months for a higher stability.Avoid freeze-thaw cycles
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide (TVLGEIKTGNSPVKQY) corresponding to amino acid sequence 174-189 from human neurotrophin 3 (NT3).
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human NT3 has a predicted length of 257 amino acids and MW of 29 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect NT3 in paraffin embedded tissues. Antigen retrieval by boiling the paraffin / formalin section in 10mM citrate buffer, pH 6.0 for 20 minutes is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The Biosensis rat neurotrophin 3 (NT-3) ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody specific for rat NT-3 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, and then a biotinylated detection polyclonal antibody specific for mouse NT-3 is added subsequently followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates washed away again with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP driven enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that is changed into yellow by the addition of an acid stop solution. The intensity of this colored product is directly proportional to the concentration of NT-3 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse NT-3 in cell culture supernatants, and serum samples only when used as directed. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human, Mouse, Rat
Immunogen:
The standard and immunogen in this assays is produced in and purified from an insect expression system consisting of amino acids Y139-T257
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Human; Mouse; and Rat. Less than 2.5% reactivity with NT-4 and no detectable cross-reactivity with other cytokines. |
The Biosensis rat neurotrophin 3 (NT-3) ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody specific for rat NT-3 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, and then a biotinylated detection polyclonal antibody specific for mouse NT-3 is added subsequently followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates washed away again with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP driven enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that is changed into yellow by the addition of an acid stop solution. The intensity of this colored product is directly proportional to the concentration of NT-3 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse NT-3 in cell culture supernatants, and serum samples only when used as directed. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Product Type:
ELISA
Antibody Type:
Monoclonal-Polyclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 4 °C
Species Reactivity:
Human, Mouse, Rat
Immunogen:
The antigen and standard in this rat NT-3 ELISA is expressed and purified from an insect cell expression sf21 system and consists of a recombinant protein containing amino acids 139-257 of rat NT-3.
The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, substrate buffer and detailed protocols.
Shelf Life:
This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at 80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
Specificity:
Human; Mouse; and Rat. Less than 2.5% reactivity with NT-4 and no detectable cross-reactivity with other cytokines. |
FUNCTION: Seems to promotes the survival of visceral and proprioceptive sensory neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Host Animal:
Sheep
Species Reactivity:
Rat, Chicken, Human
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human, mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Reconstitute in 50 µL of sterile water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. | This antiserum is known to react with rat, chicken and human NT3.
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display sevvere movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Host Animal:
Sheep
Species Reactivity:
Rat, Chicken, Human
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human, mouse and rat NT3 protein conjugated to BSA
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. | This antiserum is known to react with rat, chicken and human NT3.
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display sevvere movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Glycerol (1:) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Host Animal:
Sheep
Species Reactivity:
Rat, Chicken, Human
Immunogen:
A synthetic peptide (YAEHKSHRGEY) as part of human, mouse and rat NT3 protein conjugated to BSA has been used as the immunogen.
IHC, ELISA, WB, inhibition of biological activity. A dilution of 1:500 to 1:2000 is recommended for IHC, western blot. For inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchse
Specificity:
A cross reactivity of less than 0.1% to mouse NGF, recombinant human BDNF and NT4/5 has been shown by dot blot. | This antiserum is known to react with rat, chicken and human NT3.
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display sevvere movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Glycerol (1:) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
IHC, ELISA, WB, inhibition of biological activity. A concentration of 1-10 µg/mL is recommended for IHC, ELISA, WB and inhibition of biological activity in vitro; 2-10 µg/mL (ED50) for in vivo use. This antiserum will neutralise NT3 but not other neurotrophins. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
A cross reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by 1-site ELISA. | This antiserum is known to react with rat, chicken and human NT3.
NT3 is a member of the neurotrophin family, that controls survival and differentiation of visceral and proprioceptive sensory neurons. NT3 is closely related to both NGF and BDNF. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display sevvere movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Brain and peripheral tissues. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
IHC, ELISA, WB, dot blot, inhibition of biological activity. A dilution of 1:200 to 1:2000 is recommended for IHC, ELISA and western blot. For inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
A cross reactivity of less than 1% to mouse NGF, recombinant human BDNF and 5% to NT4/5 has been shown by 1-site ELISA. | This antiserum is known to react with rat, chicken and human NT3.
Purity:
Whole serum
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