The NEXTflex RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with RNA-Seq kits, directional RNA-Seq kits, Rapid RNA-Seq kits and the Rapid Directional RNA-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® Unique Dual Index Barcodes for NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
For multiplexing RNA-seq libraries compatible with both paired-end and single-read Illumina® sequencing
Every lot is functionally validated and tested for index purity by sequencing
Available for off-the-shelf use with NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
Ready-to-Go UDI Barcodes for RNA-Seq Applications
The NEXTFLEX® RNA-seq 2.0 UDI barcodes (up to 384) are barcoded adapters that offer robust multiplexing capabilities for RNA-seq on Illumina® platforms. These barcodes (8-nt index) are conveniently plated at 6.25 µM stock concentration to be used with the NEXTFLEX® Rapid Directional RNA-Seq kit 2.0.
For labs who want greater multiplexing capacity, we also offer a set of 1,536 RNA-seq 2.0 UDI barcodes.
Are you looking for UDI adapters for DNA-seq applications? We have a set of 1536 UDI adapters for those looking for ultra high-throughput multiplexing for their DNA libraries as well.
Optimized to Improve Data Integrity
The NEXTFLEX® Unique Dual Index barcodes contain two unique indexes on each adapter, with a hamming distance of at least three throughout the entire series, to allow error correction. The NEXTFLEX® Unique Dual Index RNA-seq barcodes can be used for PCR-free RNA sequencing applications. Patterned flow cells found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity. Every lot is functionally validated and tested for index purity by sequencing.
The NEXTflex RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with RNA-Seq kits, directional RNA-Seq kits, Rapid RNA-Seq kits and the Rapid Directional RNA-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® Unique Dual Index Barcodes for NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
For multiplexing RNA-seq libraries compatible with both paired-end and single-read Illumina® sequencing
Every lot is functionally validated and tested for index purity by sequencing
Available for off-the-shelf use with NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
Ready-to-Go UDI Barcodes for RNA-Seq Applications
The NEXTFLEX® RNA-seq 2.0 UDI barcodes (up to 384) are barcoded adapters that offer robust multiplexing capabilities for RNA-seq on Illumina® platforms. These barcodes (8-nt index) are conveniently plated at 6.25 µM stock concentration to be used with the NEXTFLEX® Rapid Directional RNA-Seq kit 2.0.
For labs who want greater multiplexing capacity, we also offer a set of 1,536 RNA-seq 2.0 UDI barcodes.
Are you looking for UDI adapters for DNA-seq applications? We have a set of 1536 UDI adapters for those looking for ultra high-throughput multiplexing for their DNA libraries as well.
Optimized to Improve Data Integrity
The NEXTFLEX® Unique Dual Index barcodes contain two unique indexes on each adapter, with a hamming distance of at least three throughout the entire series, to allow error correction. The NEXTFLEX® Unique Dual Index RNA-seq barcodes can be used for PCR-free RNA sequencing applications. Patterned flow cells found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity. Every lot is functionally validated and tested for index purity by sequencing.
The NEXTflex RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with RNA-Seq kits, directional RNA-Seq kits, Rapid RNA-Seq kits and the Rapid Directional RNA-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® Unique Dual Index Barcodes for NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
For multiplexing RNA-seq libraries compatible with both paired-end and single-read Illumina® sequencing
Every lot is functionally validated and tested for index purity by sequencing
Available for off-the-shelf use with NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
Ready-to-Go UDI Barcodes for RNA-Seq Applications
The NEXTFLEX® RNA-seq 2.0 UDI barcodes (up to 384) are barcoded adapters that offer robust multiplexing capabilities for RNA-seq on Illumina® platforms. These barcodes (8-nt index) are conveniently plated at 6.25 µM stock concentration to be used with the NEXTFLEX® Rapid Directional RNA-Seq kit 2.0.
For labs who want greater multiplexing capacity, we also offer a set of 1,536 RNA-seq 2.0 UDI barcodes.
Are you looking for UDI adapters for DNA-seq applications? We have a set of 1536 UDI adapters for those looking for ultra high-throughput multiplexing for their DNA libraries as well.
Optimized to Improve Data Integrity
The NEXTFLEX® Unique Dual Index barcodes contain two unique indexes on each adapter, with a hamming distance of at least three throughout the entire series, to allow error correction. The NEXTFLEX® Unique Dual Index RNA-seq barcodes can be used for PCR-free RNA sequencing applications. Patterned flow cells found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity. Every lot is functionally validated and tested for index purity by sequencing.
The NEXTflex RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with RNA-Seq kits, directional RNA-Seq kits, Rapid RNA-Seq kits and the Rapid Directional RNA-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® Unique Dual Index Barcodes for NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
For multiplexing RNA-seq libraries compatible with both paired-end and single-read Illumina® sequencing
Every lot is functionally validated and tested for index purity by sequencing
Available for off-the-shelf use with NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
Ready-to-Go UDI Barcodes for RNA-Seq Applications
The NEXTFLEX® RNA-seq 2.0 UDI barcodes (up to 384) are barcoded adapters that offer robust multiplexing capabilities for RNA-seq on Illumina® platforms. These barcodes (8-nt index) are conveniently plated at 6.25 µM stock concentration to be used with the NEXTFLEX® Rapid Directional RNA-Seq kit 2.0.
For labs who want greater multiplexing capacity, we also offer a set of 1,536 RNA-seq 2.0 UDI barcodes.
Are you looking for UDI adapters for DNA-seq applications? We have a set of 1536 UDI adapters for those looking for ultra high-throughput multiplexing for their DNA libraries as well.
Optimized to Improve Data Integrity
The NEXTFLEX® Unique Dual Index barcodes contain two unique indexes on each adapter, with a hamming distance of at least three throughout the entire series, to allow error correction. The NEXTFLEX® Unique Dual Index RNA-seq barcodes can be used for PCR-free RNA sequencing applications. Patterned flow cells found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity. Every lot is functionally validated and tested for index purity by sequencing.
The NEXTflex RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with RNA-Seq kits, directional RNA-Seq kits, Rapid RNA-Seq kits and the Rapid Directional RNA-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® Unique Dual Index Barcodes for NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
For multiplexing RNA-seq libraries compatible with both paired-end and single-read Illumina® sequencing
Every lot is functionally validated and tested for index purity by sequencing
Available for off-the-shelf use with NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
Ready-to-Go UDI Barcodes for RNA-Seq Applications
The NEXTFLEX® RNA-seq 2.0 UDI barcodes (up to 384) are barcoded adapters that offer robust multiplexing capabilities for RNA-seq on Illumina® platforms. These barcodes (8-nt index) are conveniently plated at 6.25 µM stock concentration to be used with the NEXTFLEX® Rapid Directional RNA-Seq kit 2.0.
For labs who want greater multiplexing capacity, we also offer a set of 1,536 RNA-seq 2.0 UDI barcodes.
Are you looking for UDI adapters for DNA-seq applications? We have a set of 1536 UDI adapters for those looking for ultra high-throughput multiplexing for their DNA libraries as well.
Optimized to Improve Data Integrity
The NEXTFLEX® Unique Dual Index barcodes contain two unique indexes on each adapter, with a hamming distance of at least three throughout the entire series, to allow error correction. The NEXTFLEX® Unique Dual Index RNA-seq barcodes can be used for PCR-free RNA sequencing applications. Patterned flow cells found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity. Every lot is functionally validated and tested for index purity by sequencing.
The NEXTflex RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with RNA-Seq kits, directional RNA-Seq kits, Rapid RNA-Seq kits and the Rapid Directional RNA-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® Unique Dual Index Barcodes for NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
For multiplexing RNA-seq libraries compatible with both paired-end and single-read Illumina® sequencing
Every lot is functionally validated and tested for index purity by sequencing
Available for off-the-shelf use with NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
Ready-to-Go UDI Barcodes for RNA-Seq Applications
The NEXTFLEX® RNA-seq 2.0 UDI barcodes (up to 384) are barcoded adapters that offer robust multiplexing capabilities for RNA-seq on Illumina® platforms. These barcodes (8-nt index) are conveniently plated at 6.25 µM stock concentration to be used with the NEXTFLEX® Rapid Directional RNA-Seq kit 2.0.
For labs who want greater multiplexing capacity, we also offer a set of 1,536 RNA-seq 2.0 UDI barcodes.
Are you looking for UDI adapters for DNA-seq applications? We have a set of 1536 UDI adapters for those looking for ultra high-throughput multiplexing for their DNA libraries as well.
Optimized to Improve Data Integrity
The NEXTFLEX® Unique Dual Index barcodes contain two unique indexes on each adapter, with a hamming distance of at least three throughout the entire series, to allow error correction. The NEXTFLEX® Unique Dual Index RNA-seq barcodes can be used for PCR-free RNA sequencing applications. Patterned flow cells found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity. Every lot is functionally validated and tested for index purity by sequencing.
The NEXTflex RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique barcodes, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice. These barcodes can be used with RNA-Seq kits, directional RNA-Seq kits, Rapid RNA-Seq kits and the Rapid Directional RNA-Seq kits.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® Unique Dual Index Barcodes for NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
For multiplexing RNA-seq libraries compatible with both paired-end and single-read Illumina® sequencing
Every lot is functionally validated and tested for index purity by sequencing
Available for off-the-shelf use with NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
Ready-to-Go UDI Barcodes for RNA-Seq Applications
The NEXTFLEX® RNA-seq 2.0 UDI barcodes (up to 384) are barcoded adapters that offer robust multiplexing capabilities for RNA-seq on Illumina® platforms. These barcodes (8-nt index) are conveniently plated at 6.25 µM stock concentration to be used with the NEXTFLEX® Rapid Directional RNA-Seq kit 2.0.
For labs who want greater multiplexing capacity, we also offer a set of 1,536 RNA-seq 2.0 UDI barcodes.
Are you looking for UDI adapters for DNA-seq applications? We have a set of 1536 UDI adapters for those looking for ultra high-throughput multiplexing for their DNA libraries as well.
Optimized to Improve Data Integrity
The NEXTFLEX® Unique Dual Index barcodes contain two unique indexes on each adapter, with a hamming distance of at least three throughout the entire series, to allow error correction. The NEXTFLEX® Unique Dual Index RNA-seq barcodes can be used for PCR-free RNA sequencing applications. Patterned flow cells found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity. Every lot is functionally validated and tested for index purity by sequencing.
The patent pending NEXTflex Rapid Directional qRNA-Seq Kit enables high precision directional gene expression analysis by RNA-seq. Developed in conjunction with Cellular Research Inc., this kit generates directional libraries equivalent to conventional RNA-Seq libraries, but with the added feature of molecular indexing. The NEXTflex Rapid Directional qRNA-Seq Kit can be used to obtain "stranded" information which identifies the specific DNA strands a given RNA transcript was derived from.
[MOLECULAR LABELING OF INDIVIDUAL DNA MOLECULES]
Similar to conventional RNA-Seq, sample RNA is converted to cDNA fragments. But prior to any PCR amplification steps, all DNA fragment ends are ligated to a pair of adapters chosen at random from a total set of 9,216 molecular indices. Individual DNA molecules of identical sequence become distinct through indexing, allowing for differentiation between re-sampling of the same molecule and sampling of a different molecule of identical sequence. Analysis using molecular indexing information provides an absolute, digital measurement of gene expression levels, irrespective of common amplification distortions observed in many directional RNA-Seq experiments.
The NEXTflex Rapid Directional qRNA-Seq Kit contains two sets of 96 distinct molecular labels on the sequencing adapters. Each label or index consists of an 8 nucleotide barcode tag. In the ligation reaction, these 96 adapters are present in vast molar excess over the concentration of the cDNA fragments, and therefore serve as a non-depleting reservoir of molecular labels. Each end of a cDNA fragment independently and randomly chooses and ligates to a single label from this pool of 96 adapters to result in a total of 96 x 96 = 9,216 possible combinations across both ends. For every clone sequenced, paired-end reads reveals the chosen label on each end along with adjoining cDNA sequence. In addition to encoding DNA fragments at the molecular level, the kit also allows for the application of sample-specific barcodes during the library preparation PCR step.
[PROTOCOL]
Using the NEXTflex Rapid Directional qRNA-Seq Kit, mRNA or rRNA-depleted RNA is fragmented using a cationic buffer. Fragmented RNA undergoes first and second strand synthesis, followed by adenylation, ligation and PCR.
This kit incorporates the NEXTflex Rapid Reverse Transcriptase, a robust, thermostable RT that outperforms other RTs on the market for NGS applications.
[MULTIPLEXING]
NEXTflex Rapid Directional qRNA-Seq Kits contain barcodes so additional barcodes do not need to be purchased. The 8 rxn kit includes 4 barcodes for multiplexing. Four different versions of the 48 reaction kit are available, each contain 24 unique barcodes. Up to 96 samples can be multiplexed together.
[MAGNETIC BEADS FOR mRNA PURIFICATION]
The NEXTflex Poly(A) Beads now provide a convenient method for batch purification of pure, intact mRNA upstream of NEXTflex Rapid Directional qRNA-Seq library preparation. NEXTflex Poly(A) Beads use oligo(dT) primer to isolate polyadenylated messenger RNAs from 1 µg 10 µg of previously isolated total RNA
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS] >>> Generates stranded, molecular indexed libraries >>> Enables high precision measurement of RNA concentration >>> Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads >>> Input 10 - 100 ng of mRNA or rRNA-depleted total RNA >>> Embedded sample index barcodes for multiplexing of up to 96 samples >>> Automation-friendly workflow is compatible with liquid handlers >>> Functionally validated with Illumina sequencing platforms
The NEXTflex Rapid Directional qRNA-Seq Kit contains enough material to prepare 8 or 48 RNA samples for Illumina compatible paired end sequencing. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5130-01D
The patent pending NEXTflex Rapid Directional qRNA-Seq Kit enables high precision directional gene expression analysis by RNA-seq. Developed in conjunction with Cellular Research Inc., this kit generates directional libraries equivalent to conventional RNA-Seq libraries, but with the added feature of molecular indexing. The NEXTflex Rapid Directional qRNA-Seq Kit can be used to obtain "stranded" information which identifies the specific DNA strands a given RNA transcript was derived from.
[MOLECULAR LABELING OF INDIVIDUAL DNA MOLECULES]
Similar to conventional RNA-Seq, sample RNA is converted to cDNA fragments. But prior to any PCR amplification steps, all DNA fragment ends are ligated to a pair of adapters chosen at random from a total set of 9,216 molecular indices. Individual DNA molecules of identical sequence become distinct through indexing, allowing for differentiation between re-sampling of the same molecule and sampling of a different molecule of identical sequence. Analysis using molecular indexing information provides an absolute, digital measurement of gene expression levels, irrespective of common amplification distortions observed in many directional RNA-Seq experiments.
The NEXTflex Rapid Directional qRNA-Seq Kit contains two sets of 96 distinct molecular labels on the sequencing adapters. Each label or index consists of an 8 nucleotide barcode tag. In the ligation reaction, these 96 adapters are present in vast molar excess over the concentration of the cDNA fragments, and therefore serve as a non-depleting reservoir of molecular labels. Each end of a cDNA fragment independently and randomly chooses and ligates to a single label from this pool of 96 adapters to result in a total of 96 x 96 = 9,216 possible combinations across both ends. For every clone sequenced, paired-end reads reveals the chosen label on each end along with adjoining cDNA sequence. In addition to encoding DNA fragments at the molecular level, the kit also allows for the application of sample-specific barcodes during the library preparation PCR step.
[PROTOCOL]
Using the NEXTflex Rapid Directional qRNA-Seq Kit, mRNA or rRNA-depleted RNA is fragmented using a cationic buffer. Fragmented RNA undergoes first and second strand synthesis, followed by adenylation, ligation and PCR.
This kit incorporates the NEXTflex Rapid Reverse Transcriptase, a robust, thermostable RT that outperforms other RTs on the market for NGS applications.
[MULTIPLEXING]
NEXTflex Rapid Directional qRNA-Seq Kits contain barcodes so additional barcodes do not need to be purchased. The 8 rxn kit includes 4 barcodes for multiplexing. Four different versions of the 48 reaction kit are available, each contain 24 unique barcodes. Up to 96 samples can be multiplexed together.
[MAGNETIC BEADS FOR mRNA PURIFICATION]
The NEXTflex Poly(A) Beads now provide a convenient method for batch purification of pure, intact mRNA upstream of NEXTflex Rapid Directional qRNA-Seq library preparation. NEXTflex Poly(A) Beads use oligo(dT) primer to isolate polyadenylated messenger RNAs from 1 µg 10 µg of previously isolated total RNA
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS] >>> Generates stranded, molecular indexed libraries >>> Enables high precision measurement of RNA concentration >>> Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads >>> Input 10 - 100 ng of mRNA or rRNA-depleted total RNA >>> Embedded sample index barcodes for multiplexing of up to 96 samples >>> Automation-friendly workflow is compatible with liquid handlers >>> Functionally validated with Illumina sequencing platforms
The NEXTflex Rapid Directional qRNA-Seq Kit contains enough material to prepare 8 or 48 RNA samples for Illumina compatible paired end sequencing. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5130-05D
The patent pending NEXTflex Rapid Directional qRNA-Seq Kit enables high precision directional gene expression analysis by RNA-seq. Developed in conjunction with Cellular Research Inc., this kit generates directional libraries equivalent to conventional RNA-Seq libraries, but with the added feature of molecular indexing. The NEXTflex Rapid Directional qRNA-Seq Kit can be used to obtain "stranded" information which identifies the specific DNA strands a given RNA transcript was derived from.
[MOLECULAR LABELING OF INDIVIDUAL DNA MOLECULES]
Similar to conventional RNA-Seq, sample RNA is converted to cDNA fragments. But prior to any PCR amplification steps, all DNA fragment ends are ligated to a pair of adapters chosen at random from a total set of 9,216 molecular indices. Individual DNA molecules of identical sequence become distinct through indexing, allowing for differentiation between re-sampling of the same molecule and sampling of a different molecule of identical sequence. Analysis using molecular indexing information provides an absolute, digital measurement of gene expression levels, irrespective of common amplification distortions observed in many directional RNA-Seq experiments.
The NEXTflex Rapid Directional qRNA-Seq Kit contains two sets of 96 distinct molecular labels on the sequencing adapters. Each label or index consists of an 8 nucleotide barcode tag. In the ligation reaction, these 96 adapters are present in vast molar excess over the concentration of the cDNA fragments, and therefore serve as a non-depleting reservoir of molecular labels. Each end of a cDNA fragment independently and randomly chooses and ligates to a single label from this pool of 96 adapters to result in a total of 96 x 96 = 9,216 possible combinations across both ends. For every clone sequenced, paired-end reads reveals the chosen label on each end along with adjoining cDNA sequence. In addition to encoding DNA fragments at the molecular level, the kit also allows for the application of sample-specific barcodes during the library preparation PCR step.
[PROTOCOL]
Using the NEXTflex Rapid Directional qRNA-Seq Kit, mRNA or rRNA-depleted RNA is fragmented using a cationic buffer. Fragmented RNA undergoes first and second strand synthesis, followed by adenylation, ligation and PCR.
This kit incorporates the NEXTflex Rapid Reverse Transcriptase, a robust, thermostable RT that outperforms other RTs on the market for NGS applications.
[MULTIPLEXING]
NEXTflex Rapid Directional qRNA-Seq Kits contain barcodes so additional barcodes do not need to be purchased. The 8 rxn kit includes 4 barcodes for multiplexing. Four different versions of the 48 reaction kit are available, each contain 24 unique barcodes. Up to 96 samples can be multiplexed together.
[MAGNETIC BEADS FOR mRNA PURIFICATION]
The NEXTflex Poly(A) Beads now provide a convenient method for batch purification of pure, intact mRNA upstream of NEXTflex Rapid Directional qRNA-Seq library preparation. NEXTflex Poly(A) Beads use oligo(dT) primer to isolate polyadenylated messenger RNAs from 1 µg 10 µg of previously isolated total RNA
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS] >>> Generates stranded, molecular indexed libraries >>> Enables high precision measurement of RNA concentration >>> Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads >>> Input 10 - 100 ng of mRNA or rRNA-depleted total RNA >>> Embedded sample index barcodes for multiplexing of up to 96 samples >>> Automation-friendly workflow is compatible with liquid handlers >>> Functionally validated with Illumina sequencing platforms
The NEXTflex Rapid Directional qRNA-Seq Kit contains enough material to prepare 8 or 48 RNA samples for Illumina compatible paired end sequencing. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5130-04D
The patent pending NEXTflex Rapid Directional qRNA-Seq Kit enables high precision directional gene expression analysis by RNA-seq. Developed in conjunction with Cellular Research Inc., this kit generates directional libraries equivalent to conventional RNA-Seq libraries, but with the added feature of molecular indexing. The NEXTflex Rapid Directional qRNA-Seq Kit can be used to obtain "stranded" information which identifies the specific DNA strands a given RNA transcript was derived from.
[MOLECULAR LABELING OF INDIVIDUAL DNA MOLECULES]
Similar to conventional RNA-Seq, sample RNA is converted to cDNA fragments. But prior to any PCR amplification steps, all DNA fragment ends are ligated to a pair of adapters chosen at random from a total set of 9,216 molecular indices. Individual DNA molecules of identical sequence become distinct through indexing, allowing for differentiation between re-sampling of the same molecule and sampling of a different molecule of identical sequence. Analysis using molecular indexing information provides an absolute, digital measurement of gene expression levels, irrespective of common amplification distortions observed in many directional RNA-Seq experiments.
The NEXTflex Rapid Directional qRNA-Seq Kit contains two sets of 96 distinct molecular labels on the sequencing adapters. Each label or index consists of an 8 nucleotide barcode tag. In the ligation reaction, these 96 adapters are present in vast molar excess over the concentration of the cDNA fragments, and therefore serve as a non-depleting reservoir of molecular labels. Each end of a cDNA fragment independently and randomly chooses and ligates to a single label from this pool of 96 adapters to result in a total of 96 x 96 = 9,216 possible combinations across both ends. For every clone sequenced, paired-end reads reveals the chosen label on each end along with adjoining cDNA sequence. In addition to encoding DNA fragments at the molecular level, the kit also allows for the application of sample-specific barcodes during the library preparation PCR step.
[PROTOCOL]
Using the NEXTflex Rapid Directional qRNA-Seq Kit, mRNA or rRNA-depleted RNA is fragmented using a cationic buffer. Fragmented RNA undergoes first and second strand synthesis, followed by adenylation, ligation and PCR.
This kit incorporates the NEXTflex Rapid Reverse Transcriptase, a robust, thermostable RT that outperforms other RTs on the market for NGS applications.
[MULTIPLEXING]
NEXTflex Rapid Directional qRNA-Seq Kits contain barcodes so additional barcodes do not need to be purchased. The 8 rxn kit includes 4 barcodes for multiplexing. Four different versions of the 48 reaction kit are available, each contain 24 unique barcodes. Up to 96 samples can be multiplexed together.
[MAGNETIC BEADS FOR mRNA PURIFICATION]
The NEXTflex Poly(A) Beads now provide a convenient method for batch purification of pure, intact mRNA upstream of NEXTflex Rapid Directional qRNA-Seq library preparation. NEXTflex Poly(A) Beads use oligo(dT) primer to isolate polyadenylated messenger RNAs from 1 µg 10 µg of previously isolated total RNA
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS] >>> Generates stranded, molecular indexed libraries >>> Enables high precision measurement of RNA concentration >>> Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads >>> Input 10 - 100 ng of mRNA or rRNA-depleted total RNA >>> Embedded sample index barcodes for multiplexing of up to 96 samples >>> Automation-friendly workflow is compatible with liquid handlers >>> Functionally validated with Illumina sequencing platforms
The NEXTflex Rapid Directional qRNA-Seq Kit contains enough material to prepare 8 or 48 RNA samples for Illumina compatible paired end sequencing. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5130-03D
The patent pending NEXTflex Rapid Directional qRNA-Seq Kit enables high precision directional gene expression analysis by RNA-seq. Developed in conjunction with Cellular Research Inc., this kit generates directional libraries equivalent to conventional RNA-Seq libraries, but with the added feature of molecular indexing. The NEXTflex Rapid Directional qRNA-Seq Kit can be used to obtain "stranded" information which identifies the specific DNA strands a given RNA transcript was derived from.
[MOLECULAR LABELING OF INDIVIDUAL DNA MOLECULES]
Similar to conventional RNA-Seq, sample RNA is converted to cDNA fragments. But prior to any PCR amplification steps, all DNA fragment ends are ligated to a pair of adapters chosen at random from a total set of 9,216 molecular indices. Individual DNA molecules of identical sequence become distinct through indexing, allowing for differentiation between re-sampling of the same molecule and sampling of a different molecule of identical sequence. Analysis using molecular indexing information provides an absolute, digital measurement of gene expression levels, irrespective of common amplification distortions observed in many directional RNA-Seq experiments.
The NEXTflex Rapid Directional qRNA-Seq Kit contains two sets of 96 distinct molecular labels on the sequencing adapters. Each label or index consists of an 8 nucleotide barcode tag. In the ligation reaction, these 96 adapters are present in vast molar excess over the concentration of the cDNA fragments, and therefore serve as a non-depleting reservoir of molecular labels. Each end of a cDNA fragment independently and randomly chooses and ligates to a single label from this pool of 96 adapters to result in a total of 96 x 96 = 9,216 possible combinations across both ends. For every clone sequenced, paired-end reads reveals the chosen label on each end along with adjoining cDNA sequence. In addition to encoding DNA fragments at the molecular level, the kit also allows for the application of sample-specific barcodes during the library preparation PCR step.
[PROTOCOL]
Using the NEXTflex Rapid Directional qRNA-Seq Kit, mRNA or rRNA-depleted RNA is fragmented using a cationic buffer. Fragmented RNA undergoes first and second strand synthesis, followed by adenylation, ligation and PCR.
This kit incorporates the NEXTflex Rapid Reverse Transcriptase, a robust, thermostable RT that outperforms other RTs on the market for NGS applications.
[MULTIPLEXING]
NEXTflex Rapid Directional qRNA-Seq Kits contain barcodes so additional barcodes do not need to be purchased. The 8 rxn kit includes 4 barcodes for multiplexing. Four different versions of the 48 reaction kit are available, each contain 24 unique barcodes. Up to 96 samples can be multiplexed together.
[MAGNETIC BEADS FOR mRNA PURIFICATION]
The NEXTflex Poly(A) Beads now provide a convenient method for batch purification of pure, intact mRNA upstream of NEXTflex Rapid Directional qRNA-Seq library preparation. NEXTflex Poly(A) Beads use oligo(dT) primer to isolate polyadenylated messenger RNAs from 1 µg 10 µg of previously isolated total RNA
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS] >>> Generates stranded, molecular indexed libraries >>> Enables high precision measurement of RNA concentration >>> Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads >>> Input 10 - 100 ng of mRNA or rRNA-depleted total RNA >>> Embedded sample index barcodes for multiplexing of up to 96 samples >>> Automation-friendly workflow is compatible with liquid handlers >>> Functionally validated with Illumina sequencing platforms
The NEXTflex Rapid Directional qRNA-Seq Kit contains enough material to prepare 8 or 48 RNA samples for Illumina compatible paired end sequencing. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5130-02D
Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples intended for use to produce libraries for NGS RNA-Seq experiments. Removal of rRNA avoids the waste of reagents and bioinformatic resources to sequence and align the ~85% of total RNA comprised of rRNA, which is generally not a target of interest in NGS sequencing experiments.
The NEXTflex Poly(A) Beads 2.0 offer improved mRNA yields, lower rRNA contamination, and a more simplified protocol, verified from 10 ng - 5 µg of total RNA for a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep.
Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. These beads have been validated with the new and improved NEXTflex Rapid Directional RNA-Seq Kit 2.0.
The NEXTflex Poly(A) beads 2.0 are an easy and cost-effective method for removing rRNA in RNA-Seq libraries. This product takes advantage of the tract of adenosine residues present at the 3 ends of the vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines (oligo dT) immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.
The NEXTflex Poly(A) Beads 2.0 contains enough material to extract mRNA from 8, 48 or 96 total RNA samples for subsequent RNA-seq library prep or other applications. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at 4°C.
These beads have been validated with the following kits: NEXTflex Directional RNA-Seq Kit NEXTflex Directional RNA-Seq Kit 2.0
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples intended for use to produce libraries for NGS RNA-Seq experiments. Removal of rRNA avoids the waste of reagents and bioinformatic resources to sequence and align the ~85% of total RNA comprised of rRNA, which is generally not a target of interest in NGS sequencing experiments.
The NEXTflex Poly(A) Beads 2.0 offer improved mRNA yields, lower rRNA contamination, and a more simplified protocol, verified from 10 ng - 5 µg of total RNA for a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep.
Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. These beads have been validated with the new and improved NEXTflex Rapid Directional RNA-Seq Kit 2.0.
The NEXTflex Poly(A) beads 2.0 are an easy and cost-effective method for removing rRNA in RNA-Seq libraries. This product takes advantage of the tract of adenosine residues present at the 3 ends of the vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines (oligo dT) immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.
The NEXTflex Poly(A) Beads 2.0 contains enough material to extract mRNA from 8, 48 or 96 total RNA samples for subsequent RNA-seq library prep or other applications. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at 4°C.
These beads have been validated with the following kits: NEXTflex Directional RNA-Seq Kit NEXTflex Directional RNA-Seq Kit 2.0
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples intended for use to produce libraries for NGS RNA-Seq experiments. Removal of rRNA avoids the waste of reagents and bioinformatic resources to sequence and align the ~85% of total RNA comprised of rRNA, which is generally not a target of interest in NGS sequencing experiments.
The NEXTflex Poly(A) Beads 2.0 offer improved mRNA yields, lower rRNA contamination, and a more simplified protocol, verified from 10 ng - 5 µg of total RNA for a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep.
Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. These beads have been validated with the new and improved NEXTflex Rapid Directional RNA-Seq Kit 2.0.
The NEXTflex Poly(A) beads 2.0 are an easy and cost-effective method for removing rRNA in RNA-Seq libraries. This product takes advantage of the tract of adenosine residues present at the 3 ends of the vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines (oligo dT) immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.
The NEXTflex Poly(A) Beads 2.0 contains enough material to extract mRNA from 8, 48 or 96 total RNA samples for subsequent RNA-seq library prep or other applications. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at 4°C.
These beads have been validated with the following kits: NEXTflex Directional RNA-Seq Kit NEXTflex Directional RNA-Seq Kit 2.0
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples intended for use to produce libraries for NGS RNA-Seq experiments. Removal of rRNA avoids the waste of reagents and bioinformatic resources to sequence and align the ~85% of total RNA comprised of rRNA, which is generally not a target of interest in NGS sequencing experiments.
The NEXTflex Poly(A) Beads 2.0 offer improved mRNA yields, lower rRNA contamination, and a more simplified protocol, verified from 10 ng - 5 µg of total RNA for a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep.
Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. These beads have been validated with the new and improved NEXTflex Rapid Directional RNA-Seq Kit 2.0.
The NEXTflex Poly(A) beads 2.0 are an easy and cost-effective method for removing rRNA in RNA-Seq libraries. This product takes advantage of the tract of adenosine residues present at the 3 ends of the vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines (oligo dT) immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.
The NEXTflex Poly(A) Beads 2.0 contains enough material to extract mRNA from 8, 48 or 96 total RNA samples for subsequent RNA-seq library prep or other applications. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at 4°C.
These beads have been validated with the following kits: NEXTflex Directional RNA-Seq Kit NEXTflex Directional RNA-Seq Kit 2.0
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
The NEXTflex Poly(A) Beads (magnetic separation beads) provide a convenient method for batch purification of pure, intact mRNA upstream of library preparation for RNA-Seq. NEXTflex Poly(A) Beads beads use oligo(dT) primer to isolate polyadenylated messenger RNAs from 1 µg 10 µg of previously isolated total RNA. Separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. Ease of use and consistent results makes the NEXTflex Poly(A) Beads ideal for NGS library preparation applications.
These beads have been validated with the following kits:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512981
The NEXTflex Poly(A) Beads (magnetic separation beads) provide a convenient method for batch purification of pure, intact mRNA upstream of library preparation for RNA-Seq. NEXTflex Poly(A) Beads beads use oligo(dT) primer to isolate polyadenylated messenger RNAs from 1 µg 10 µg of previously isolated total RNA. Separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. Ease of use and consistent results makes the NEXTflex Poly(A) Beads ideal for NGS library preparation applications.
These beads have been validated with the following kits:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512979
The NEXTflex Poly(A) Beads (magnetic separation beads) provide a convenient method for batch purification of pure, intact mRNA upstream of library preparation for RNA-Seq. NEXTflex Poly(A) Beads beads use oligo(dT) primer to isolate polyadenylated messenger RNAs from 1 µg 10 µg of previously isolated total RNA. Separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. Ease of use and consistent results makes the NEXTflex Poly(A) Beads ideal for NGS library preparation applications.
These beads have been validated with the following kits:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 512980
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