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Product Search Results: M-1818

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Product Search Results: M-1818

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Biosensis M-1818-100 100 µg £298.00
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Product Detail: Mouse anti-p75 neurotrophin receptor (p75NTR) Monoclonal Antibody (Unconjugated), suitable for FC, ICC, IHC-Frozen, WB, IP, Immunopanning.
Background Info: p75NTR (CD271) was originally discovered as a low affinity nerve growth factor receptor (NGFR). Later it was found that it was the receptor for all neurotrophins, including NGF, BDNF, NT3 and NT4/5. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. Recent research proposes the extracellular domain of p75NTR as a biomarker for monitoring the progression of motor neuron disease (MND), also known as Amyotrophic Lateral Sclerosis (ALS) or Lou Gehrig's Disease. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3.
Product Type: Antibody
Antibody Type: Monoclonal
Format: Lyophilized from a solution containing PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives.
Host Animal: Mouse
Species Reactivity: Human,Mouse,Rat
Immunogen: Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.
Applications: FC,ICC,IHC-Frozen,Immunopanning,IP,WB
Clone number: 8J2
Antibody Isotype: IgG2a
Application Details: Flow Cytometry: 5-20 µg/mL. Western Blotting: 0.5-2.0 µg/mL, non-reducing conditions only (no DTT or beta-mercaptoethanol). Immunoprecipitation: lysate dependent. 10 ug per 200-500 ug total protein. Immunopanning: 1-5 µg/mL. Immunocytochemistry: 1-5 µg/mL. Staining is strongest in non-fixed cells, light fixation is tolerable. Immunohistochemistry: fresh, acetone fixed sections only, epitope is fixation sensitive. Not suitable in formalin-fixed, paraffin (FFPE) embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names: Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; CD271
Biosensis Brand: Biosensis®
Conjugate: Unconjugated
Shelf Life: 12 months after date of receipt (unopened vial).
Use: For research use only.
References: Matusica D et al. (2008). Characterisation and use of the NSC-34 cell liner for study of nerurotrophin receptor trafficking. <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/17896795?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum"> J. Neurosci. Res. 86(3) pp. 553-65. Huh CY et al. (2008). Chronic exposure to nerve growth factor increases acetylcholine and glutamate release from cholinergic neurons of the rat medial septum and diagonal band of Boca via mechanisms mediated bu p75NTR.<a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/18256260?ordinalpos=3&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum"> J. Neurosci. 28(6) pp. 1404-9. Lagares A et al. (2007). Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio-neuronal precursor maturation. <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/17652585?ordinalpos=6&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum"> J. Neurosci. 27(30) pp. 7939-53. DiStefano & Johnson (1988). Identification of a truncated form of the nerve growth factor receptor. <a class="newA" target="_blank" href="https://www.ncbi.nlm.nih.gov/pubmed/2829174"> Proc Natl Acad Sci U S A. 1988 Jan;85(1):270-4.
Specificity: Human, reacts with human, mouse and rat. Cross-reactivity with other species not tested but expected.This antibody is specific for NGFR/p75NTR as demonstRated by western blotting and immunprecipitation. The antibody recognizes extracellular p75NTR under non-reducing conditions.
Purification: Protein A purified IgG
Target: NGFR/p75 neurotrophin receptor (p75NTR)
Accession Number: P08138
Research Areas: Antibodies & Conjugates/Biosensis Neurotrophic Factor Receptors,Antibodies & Conjugates/Sensory Nervous System,Antibodies & Conjugates/Biosensis Stem Cells,Antibodies & Conjugates/Flow Cytometry Antibodies,Antibodies & Conjugates/Biosensis Amyotrophic Lateral Sclerosis