The NEXTflex Combo-Seq Kit enables the user to generate combined mRNA and small RNA libraries in a single workflow using 5 ng 100 ng of total RNA inputs, without requiring upfront costly and tedious rRNA depletion or poly(A) selection. The kit utilizes patented and patent-pending technology that allows processing of mRNA fragments and small RNA for library construction. Like the best-in-class, NEXTflex small RNA-seq kit v3, the NEXTflex Combo-Seq mRNA/miRNA library preparation kit utilizes adapters with randomized ends to greatly reduce bias compared to standard protocols. This allows a more accurate representation of small RNA in the starting material and cost-saving due to efficient sequencing. Adapter-dimer reducing techniques built into the protocol enables gel-free workflow from inputs as low as 5 ng of total RNA. As a result, the kit is streamlined and automation-friendly from start-to-finish. Libraries with robust yield can be prepared within one day using a manual or automated protocol.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - mRNA/miRNA - Illumina Compatible
Applications:
Combo-Seq mRNA/miRNA
Search Terms:
Rapid Directional mRNA Small RNA
Additional Info:
The NEXTflex Combo-Seq Kit contains enough material to prepare 48 RNA samples for Illumina next-generation sequencing platforms. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C, except: NEXTflex Adapter Depletion Solution, Resuspension Buffer, and Nuclease-free Water, which can be stored at room temperature, and NEXTflex Cleanup Beads, which should be stored at 4°C.
[MULTIPLEXING]
8 UDI barcoded primers are included in the small reaction size NEXTflex Combo-Seq kit and 48 UDI barcoded primers are included in the large reaction size kit.
KEY FEATURES Combined library prep workflow for both small RNA and mRNA sequencing Completely gel-free protocol from inputs as low as 5ng Compatible with total RNA inputs, requiring no poly(A) selection or rRNA depletion Complete kit solution, including cleanup/size selection beads and barcodes Automation-ready on the PerkinElmer Sciclone G3 NGS/NGSx workstation
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The NEXTflex Combo-Seq Kit enables the user to generate combined mRNA and small RNA libraries in a single workflow using 5 ng 100 ng of total RNA inputs, without requiring upfront costly and tedious rRNA depletion or poly(A) selection. The kit utilizes patented and patent-pending technology that allows processing of mRNA fragments and small RNA for library construction. Like the best-in-class, NEXTflex small RNA-seq kit v3, the NEXTflex Combo-Seq mRNA/miRNA library preparation kit utilizes adapters with randomized ends to greatly reduce bias compared to standard protocols. This allows a more accurate representation of small RNA in the starting material and cost-saving due to efficient sequencing. Adapter-dimer reducing techniques built into the protocol enables gel-free workflow from inputs as low as 5 ng of total RNA. As a result, the kit is streamlined and automation-friendly from start-to-finish. Libraries with robust yield can be prepared within one day using a manual or automated protocol.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - mRNA/miRNA - Illumina Compatible
Applications:
Combo-Seq mRNA/miRNA
Search Terms:
Rapid Directional mRNA Small RNA
Additional Info:
The NEXTflex Combo-Seq Kit contains enough material to prepare 48 RNA samples for Illumina next-generation sequencing platforms. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C, except: NEXTflex Adapter Depletion Solution, Resuspension Buffer, and Nuclease-free Water, which can be stored at room temperature, and NEXTflex Cleanup Beads, which should be stored at 4°C.
[MULTIPLEXING]
8 UDI barcoded primers are included in the small reaction size NEXTflex Combo-Seq kit and 48 UDI barcoded primers are included in the large reaction size kit.
KEY FEATURES Combined library prep workflow for both small RNA and mRNA sequencing Completely gel-free protocol from inputs as low as 5ng Compatible with total RNA inputs, requiring no poly(A) selection or rRNA depletion Complete kit solution, including cleanup/size selection beads and barcodes Automation-ready on the PerkinElmer Sciclone G3 NGS/NGSx workstation
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The NEXTflex Combo-Seq Kit enables the user to generate combined mRNA and small RNA libraries in a single workflow using 5 ng 100 ng of total RNA inputs, without requiring upfront costly and tedious rRNA depletion or poly(A) selection. The kit utilizes patented and patent-pending technology that allows processing of mRNA fragments and small RNA for library construction. Like the best-in-class, NEXTflex small RNA-seq kit v3, the NEXTflex Combo-Seq mRNA/miRNA library preparation kit utilizes adapters with randomized ends to greatly reduce bias compared to standard protocols. This allows a more accurate representation of small RNA in the starting material and cost-saving due to efficient sequencing. Adapter-dimer reducing techniques built into the protocol enables gel-free workflow from inputs as low as 5 ng of total RNA. As a result, the kit is streamlined and automation-friendly from start-to-finish. Libraries with robust yield can be prepared within one day using a manual or automated protocol.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - mRNA/miRNA - Illumina Compatible
Applications:
Combo-Seq mRNA/miRNA
Search Terms:
Rapid Directional mRNA Small RNA
Additional Info:
The NEXTflex Combo-Seq Kit contains enough material to prepare 48 RNA samples for Illumina next-generation sequencing platforms. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C, except: NEXTflex Adapter Depletion Solution, Resuspension Buffer, and Nuclease-free Water, which can be stored at room temperature, and NEXTflex Cleanup Beads, which should be stored at 4°C.
[MULTIPLEXING]
8 UDI barcoded primers are included in the small reaction size NEXTflex Combo-Seq kit and 48 UDI barcoded primers are included in the large reaction size kit.
KEY FEATURES Combined library prep workflow for both small RNA and mRNA sequencing Completely gel-free protocol from inputs as low as 5ng Compatible with total RNA inputs, requiring no poly(A) selection or rRNA depletion Complete kit solution, including cleanup/size selection beads and barcodes Automation-ready on the PerkinElmer Sciclone G3 NGS/NGSx workstation
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
The NEXTflex Combo-Seq Kit enables the user to generate combined mRNA and small RNA libraries in a single workflow using 5 ng 100 ng of total RNA inputs, without requiring upfront costly and tedious rRNA depletion or poly(A) selection. The kit utilizes patented and patent-pending technology that allows processing of mRNA fragments and small RNA for library construction. Like the best-in-class, NEXTflex small RNA-seq kit v3, the NEXTflex Combo-Seq mRNA/miRNA library preparation kit utilizes adapters with randomized ends to greatly reduce bias compared to standard protocols. This allows a more accurate representation of small RNA in the starting material and cost-saving due to efficient sequencing. Adapter-dimer reducing techniques built into the protocol enables gel-free workflow from inputs as low as 5 ng of total RNA. As a result, the kit is streamlined and automation-friendly from start-to-finish. Libraries with robust yield can be prepared within one day using a manual or automated protocol.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Product Type:
NGS - mRNA/miRNA - Illumina Compatible
Applications:
Combo-Seq mRNA/miRNA
Search Terms:
Rapid Directional mRNA Small RNA
Additional Info:
The NEXTflex Combo-Seq Kit contains enough material to prepare 48 RNA samples for Illumina next-generation sequencing platforms. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C, except: NEXTflex Adapter Depletion Solution, Resuspension Buffer, and Nuclease-free Water, which can be stored at room temperature, and NEXTflex Cleanup Beads, which should be stored at 4°C.
[MULTIPLEXING]
8 UDI barcoded primers are included in the small reaction size NEXTflex Combo-Seq kit and 48 UDI barcoded primers are included in the large reaction size kit.
KEY FEATURES Combined library prep workflow for both small RNA and mRNA sequencing Completely gel-free protocol from inputs as low as 5ng Compatible with total RNA inputs, requiring no poly(A) selection or rRNA depletion Complete kit solution, including cleanup/size selection beads and barcodes Automation-ready on the PerkinElmer Sciclone G3 NGS/NGSx workstation
Ordering Update:
Please note, this product must now be ordered from Perkin Elmer UK. Please email CC.UK@PERKINELMER.COM to place your order.
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