GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Rabbit anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
DYKDDDDK epitope tag (Sigma FLAG®), fused to proteins in plant cells
His-Tag is a polyhistidine tag which consists of 3 or 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
His-Tag is a polyhistidine tag which consists of 3 or 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
His-Tag is a polyhistidine tag which consists of 3 or 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Green fluorescent protein (Venus) from Aequorea victoria (Jellyfish), can be mutated to emit at different wavelengths such as blue for BFP (when Tyr-66 is replaced by His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP (when THR-203 is replaced by Tyr). It is an energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
VENUS
Immunogen:
Full length, recombinat VENUS protein, expressed in E.coli, UniProt: P42212
Green fluorescent protein (Venus) from Aequorea victoria (Jellyfish), can be mutated to emit at different wavelengths such as blue for BFP (when Tyr-66 is replaced by His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP (when THR-203 is replaced by Tyr). It is an energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
VENUS
Immunogen:
Full length, recombinat VENUS protein, expressed in E.coli, UniProt: P42212
Green fluorescent protein (Venus) from Aequorea victoria (Jellyfish), can be mutated to emit at different wavelengths such as blue for BFP (when Tyr-66 is replaced by His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP (when THR-203 is replaced by Tyr). It is an energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
VENUS
Immunogen:
Full length, recombinat VENUS protein, expressed in E.coli, UniProt: P42212
Green fluorescent protein (Venus) from Aequorea victoria (Jellyfish), can be mutated to emit at different wavelengths such as blue for BFP (when Tyr-66 is replaced by His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP (when THR-203 is replaced by Tyr). It is an energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Lyophilized antibody can be stored at -20°C for up to 3 years. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
cell lysate overexoressing Venus protein fusion
Immunogen:
Full length, recombinat VENUS protein, expressed in E.coli, UniProt: P42212
TRITC is a bright orange-fluorescent dye. The sensitivity of the TRITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. This antibody is used to enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to TRITC.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.
TRITC is a bright orange-fluorescent dye. The sensitivity of the TRITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. This antibody is used to enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to TRITC.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.
FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. FITC antibody is used to enhance the specific signal from a monoclonal antibody or a secondary antibody conjugated to FITC. The sensitivity of the FITC hapten-anti-hapten system makes it a useful alternative to other systems.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.
FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. FITC antibody is used to enhance the specific signal from a monoclonal antibody or a secondary antibody conjugated to FITC. The sensitivity of the FITC hapten-anti-hapten system makes it a useful alternative to other systems.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.
FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. FITC antibody is used to enhance the specific signal from a monoclonal antibody or a secondary antibody conjugated to FITC. The sensitivity of the FITC hapten-anti-hapten system makes it a useful alternative to other systems.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.
His-Tag is a polyhistidine tag which consists of 3 or 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. This tag has MW of 35 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
BirA (mutated/TurboID)
Immunogen:
Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG® tags.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C.
Host Animal:
Mouse
Species Reactivity:
GST-tagged proteins
Immunogen:
Glutathione-S-Transferase (GST) tagged protein from Schistosoma japonicum.
Total IgG1. Protein G purified in PBS pH 7.4, with 50% glycerol and 0.09% sodium azide.
Reconstitution:
Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes
Related products:
Plant and algal protein extraction buffer Secondary antibodies
GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG® tags.
GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG® tags.
GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG® tags.
GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG® tags.
FLAG®-tag is a tag that can be added to a protein sequence. It can be used for affinity chromatography, to separate recombinant protein from wt proteins. It can also be used to isolate protein complexes with multiple subunits.
FLAG®-tag is a tag that can be added to a protein sequence. It can be used for affinity chromatography, to separate recombinant protein from wt proteins. It can also be used to isolate protein complexes with multiple subunits.
FLAG®-tag is a tag that can be added to a protein sequence. It can be used for affinity chromatography, to separate recombinant protein from wt proteins. It can also be used to isolate protein complexes with multiple subunits.
FLAG®-tag is a tag that can be added to a protein sequence. It can be used for affinity chromatography, to separate recombinant protein from wt proteins. It can also be used to isolate protein complexes with multiple subunits.
FLAG® is a tag that can be added to a protein sequence. It can be used for affinity chromatography, to separate recombinant protein from wt proteins. It can also be used to isolate protein complexes with multiple subunits.
mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
mCherry
Immunogen:
Recombinant full length mCherry expressed and purified from E. coli.
In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector.Sequence identity between mCherry and original GFP protein is 27%. Antibody does not cross with GFP or eGFP and likely not any of the relatives in western blot.
Application Details:
1 : 250-1 : 500 (ICC), 1 : 500-1 : 1000 (WB)
Purity:
Immunogen affinity purified in PBS pH 7.4, with 3% trehalose.
mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, however it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
mCherry
Immunogen:
Recombinant full length mCherry expressed and purified from E. coli.
Applications:
Immunocytochemistry (ICC), Immunofluorescence (IF), Western blot (WB)
YFP (Yellow Fluorescent Protein) is a genetic mutant of green fluorescent protein (GFP). YFP has an excitation peak at 514 nm and emission peak at 527 nm.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C, Store in small aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Native and denatured forms of GFP and Enhanced Green Fluorescent Protein (EGFP), Yellow Fluorescent Protein (YFP), Enhanced Yellow Fluorescent Protein (EYFP) and Cyan Fluorescent Protein (CFP)
Immunogen:
KLH-conjugated synthetic peptide derived from N-terminal of YFP protein from the jellyfish Aequorea victoria, UniProt:A0A059PIR9
Applications:
ELISA (ELISA), Dot Blot, Immunoprecipitation (IP), Immunolocalization (IL), Western Blot (WB)
HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C, Store in small aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
HA-tagged proteins in transfected mammalian cells or expressed under native promoter
Immunogen:
KLH-conjugated synthetic peptide: YPYDVPDYA
Applications:
Immunoprecipitation (IP), Immunolocalization (IL), Western Blot (WB)
GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.This antibody is directly conjugated to HRP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
Native and denatured forms of purified GST or GST fusion proteins
GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.This antibody is directly conjugated to Biotin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Expected Species:
native and denatured forms of purified GST or GST fusion proteins
GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.This antibody is directly conjugated to ALP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
Native and denatured forms of purified GST or GST fusion proteins
GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Expected Species:
native and denatured forms of purified GST or GST fusion proteins
Overnight incubation of this antibody with dilution 1:5 000 with 0,5 µg of GST-tagged protein produced strong background but works fine with samples of total Escherichia coli lysate if GST-tagged protein is expressed in low amounts.
Application Details:
1: 1: 1000 (ELISA), 1: 2000 (WB)
Purity:
Immunogen affinity purified serum in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 µl of sterile water
Related products:
Plant and algal protein extraction buffer Secondary antibodies
Molecular Weight:
26 kDa
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
Exact working dilution for each application needs to be determined experimentally
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA. Alternative name: Csn1.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. For long term storage -80°C is recommended. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Cas9 from Streptococcus pyogenes
Immunogen:
Recombinant protein part from the N-terminus of Cas9 from Streptococcus pyogenes.
Applications:
Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®) and is directly conjugated to HRP, Horseradish Peroxidase. This is of advantage to shorten assay time by no need to use a secondary antibody to DYKDDDDK.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store freeze-dried poweder at 2-8°C.Rehydrate with 1.0 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily. Shelf life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Antibody is provided in: 100 mM NaPO4 (pH 7,4), 100 mM NaCl, 1,5% BSA, 50 mM Sucrose, 0,01 % Thimerosal,Affinity purified antibody has over 95% purity based on SDS-PAGE
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA. Alternative name: Csn1.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at 4°C; Please remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
Cas9 from Streptococcus pyogenes
Immunogen:
Recombinant protein part from the N-terminus of Cas9 from Streptococcus pyogenes.
Strep-tag®II is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) and this peptide sequence shows high affinity towards Strep-Tactin®, a specifically engineered streptavidin, and can be N- or C- terminally fused to recombinant proteins.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
mNeonGreen (Fluorescent Protein) is a new bright monomertic yellow-green fluorescent, with low conservation level to GFP. It has an excitation maximum at 506 nm and an emission maximum at 517 nm and therefore is compatible with the most GFP filter sets. mNeonGreen is 3x brighter compare to GFP, more stable and does not bleach so fast as GFP, which makes it very suitable for confocal and super resolution microscopy, for fusion proteins with low expression levels. It can be used in bicistronic vectors, which will allow simultaneous expression of two proteins separately, from the same RNA transcript. mNeonGreen has MW of 26.6 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
mNG tag in plant (Arabidopsis thaliana) and algal vectors (Chlamydomonas reinhardtii)
Immunogen:
KLH-conjugated peptide derived from synthetic peptide, UniProt: A0A1S4NYF2 from common lancelet Branchiostoma lanceolatum
This antibody is detecting protein sequence of mNG tag in plant and algal vectors.This antibody is also reacting to some degree with YFP and mCherry.
Application Details:
1 : 1000 - 1: 5000 (WB)
Purity:
Immunogen affinity purified serum, in PBS pH 7.4.
Reconstitution:
For reconstitution add 50 µl, of sterile water.
Related products:
Plant and algal protein extraction buffer Secondary antibodies
Molecular Weight:
Depends upon used fusion partner
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
To be added when available, antibody available in November 2021.
Special application note:
The peptide used to elicit this antibody is conserved in the following expression vectors: dCas9-P2A-DHFR(TSc3)-T2A-mNeonGreen [Cloning vector pBM020]Cas9-P2A-DHFR(TSc3)-T2A-mNeonGreen [Cloning vector pBM006]mNeonGreen4-tDeg [Cloning vector pminiCMV-mNeonGreen4-tDeg]ER-localized mNEONGREEN [Binary vector pKT-NM-erNEON]mNeonGreen-3xFLAG [Cloning vector pLM160-mNeonGreen]mNeonGreen-ty1 [Cloning vector pSAG1-mNeonGreen_TUB1-dTomato]mNeonGreen, partial [Binary vector pRATIO2131]The peptide used to elicit this antibody is not conserved in GFP protein sequence.
Beta-galactosidase is an exoglycosidase which hydrolyzes the ?-glycosidic bond formed between agalactose and its organic moiety. It may also cleave fucosides and arabinosides but with much lower efficiency. It is an essential enzyme in the human body. Deficiencies in the protein can result ingalactosialidosis or Morquio B syndrome. In E. coli , the gene of ?-galactosidase, the lacZ gene, is present as part of the inducible system lac operon which is activated in the presence of lactose when glucose level is low. It is commonly used in molecular biology as a reporter marker to monitor gene expression. It also exhibits a phenomenon called ?-complementation which forms the basis for the blue/white screening of recombinant clones. This enzyme can be split in two peptides, LacZ? and LacZ?, neither of which is active by itself but when both are present together, spontaneously reassemble into a functional enzyme. This property is exploited in many cloning vectors where the presence of the lacZ? gene in a plasmid can complement in trans another mutant gene encoding the LacZ? in specific laboratory strains of E. coli . However, when DNA fragments are inserted in the vector, the production of LacZ? is disrupted, the cells therefore show no ?-galactosidase activity. The presence or absence of an active ?-galactosidase may be detected by X-gal, which produces a characteristic blue dye when cleaved by ?-galactosidase, thereby providing an easy means of distinguishing the presence or absence of cloned product in a plasmid. E. coli ?-Galactosidase consists of 1,024 amino acids with molecular mass of 116 kDa and functional form is a homotetramer.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 2 mg/ml
Storage Temp:
Store at -20°C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. , Do not store this antibody below -20°C
Host Animal:
Rabbit
Species Reactivity:
Beta galactosidase (E.coli) and beta galactosidase tagged proteins
Immunogen:
Full length Beta galactosidase from E.coli UniProt: B7UJI9
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid at 1 mg/ml
Storage Temp:
Store at -20°C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
GST-tagged recombinant proteins
Immunogen:
Recombinant GST of Schistosoma japonicum UniProt: P08515
Applications:
Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
GST-tag (glutathione S-transferase) is a tag added to N-terminus of a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20°C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
GST-tag
Immunogen:
Recombinant, full size GST, amino acids 1-212, NCBI: AAA57089
Applications:
ELISA (ELISA), Immunoprecipitation (IP), Western blot (WB)
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20°C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
EGFP, S65T-GFP, RS-GFP, YFP
Immunogen:
Recombinant, His-tagged EGFP protein from Aequorea victoria, UniProt: P42212
Applications:
Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
Tatsumi et al. (2017). G196 epitope tag system: a novel monoclonal antibody, G196,recognizes the small, soluble peptide DLVPR with high affinity. Sci Rep. 2017 Mar 7;7:43480.doi: 10.1038/srep43480. (Immunofluorescence, Western blot)
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid at 1 mg/ml
Storage Temp:
Store at -20°C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rat
Species Reactivity:
Native GFP, Recombinant GFP (E,coli), all variants of GFP, including EGFP
Immunogen:
Recombinant GFP protein from Aequorea victoria, UniProt: P42212
Applications:
Chromatin immunoprecipitation (ChIP), ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
Immunoglobulin Protein A purified in PBS, 50 % glycerol, filter sterilized.
Selected references:
Maehara et al. (2015). issue-specific expression of histone H3 variants diversified after species separation. Epigenetics Chromatin. 2015 Sep 17;8:35. doi: 10.1186/s13072-015-0027-3. (ChIP)Okazaki et al. (2012). Nuclear localization signal in a cancer-related transcriptional regulator protein NAC1. Carcinogenesis. 2012 Oct;33(10):1854-62.doi: 10.1093/carcin/bgs193. (Immunoprecipiation)
Fluorescent proteins, like BFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at 2-8°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
BFP
Immunogen:
Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
Immunoglobulin Protein A purified in a 10 mM ammonium bicarbonate buffer, with 2 mg of BSA.
Reconstitution:
Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%). Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 Additional sodium azide ( up to 0.05%) is recommended for long term storage. For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 ?l buffer.
Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at 2-8°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
BFP, GFP, YFP
Immunogen:
Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
Immunoglobulin Protein A purified in a 10 mM ammonium bicarbonate buffer, with 2 mg of BSA.
Reconstitution:
Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%). Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 Additional sodium azide ( up to 0.05%) is recommended for long term storage. For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 ?l buffer.
HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors. An anti-HA antibody can then be used to detect the protein on western blot or to immunoprecipitate the recombinant protein in binding studies with transfected cells.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
HA-tagged proteins
Immunogen:
KLH-conjugated synthetic petide YPYDVPDYA of influenza virus hemagglutinin.
Molar F/P ratio is 3,6 and indicates an average number of FITC molecules/IgY molecule as determined by the extinction values at 495 nm and 280 nm (OD495 and OD280), respectively
HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors. An anti-HA antibody can then be used to detect the protein on western blot or to immunoprecipitate the recombinant protein in binding studies with transfected cells.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
HA-tagged proteins
Immunogen:
KLH-conjugated synthetic petide YPYDVPDYA of influenza virus hemagglutinin.
Applications:
ELISA (ELISA), Flow cytometry (Flowcyt), Western blot (WB)
His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors. An anti-HA antibody can then be used to detect the protein on western blot or to immunoprecipitate the recombinant protein in binding studies with transfected cells.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
HA-tagged proteins
Immunogen:
9 amono acid long synthetic petide to influenza virus hemagglutinin,
BC2 tag is a short and inert peptide-tag for protein studies.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Protein of interest overexpressed wth BC2-tag
Immunogen:
KLH-conjugated BC2 tag PDRKAAVSHWQQ derived from beta catenin.
Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. This tag has MW of 35 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
BirA (mutated/TurboID)
Immunogen:
Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. This tag has MW of 35 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
BirA (mutated/TurboID)
Immunogen:
Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
BirA (mutated/TurboID)
Expected Species:
mini TurboID
Immunogen:
Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
Chicken anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20°C; avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Antibody was analyzed using amino-terminal Met-Flag-BAP, amino- terminal FLAG-BAP and carboxy-terminal FLAG-BAP fusion proteins and Invitrogen Positope R900-40
MBP (Maltose binding protein) is encoded by the malE gene of E.coli and is a commonly used tag when studying protein expression using a wide range of applications. MBP tag enables easy purification of proteins from bacterial extracts under mild conditions.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20°C for up to 1 year, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
CFP (Cyan fluorescent protein) is a naturally fluorescent protein which emits blue light when excited by a 405 nm laser. This emission is optimally detected at 485 nm. It is used in laboratories as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C for up to 1 year, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Native and denaturated Cyan Fluorescent protein (CFP) and its variants: Enhanced Green Fluorescent Protein (EGFP), Yellow Fluorescent Protein (YEP)
Immunogen:
KLH-conjugated N-terminal peptide of Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria
Applications:
Dot Blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Rabbit anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20°C for up to 1 year, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
DYKDDDDK epitope tag (Sigma FLAG®), fused to proteins in plant and mammalian cells
Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C for up to 1 year, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
DYKDDDDK epitope tag (Sigma FLAG®), fused to proteins in mammalian cells
Immunoglobulin Protein A/G purified in PBS, pH 7.4. Contains 0.055% sodium azide.
Related products:
Secondary antibodies
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Durall et al. (2020). Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803. Biotechnol Biofuels. 2020 Jan 28;13:16. doi: 10.1186/s13068-020-1653-y. Wardhan et al. (2017). Chickpea transcription factor CaTLP1 interacts with protein kinases, modulates ROS accumulation and promotes ABA-mediated stomatal closure. Sci Rep. 2016 Dec 9;6:38121. doi: 10.1038/srep38121.
The anti-Myc tag is a primary antibody which is used to detect proteins containing the Myc epitope tag. The Myc tag contains the amino acid sequence EQKLISEEDL, corresponding to amino acids 410-419 of human Myc.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Myc epitope tag fused to the N- or C-terminal of proteins in transfected or transformed cells
Red fluorescent protein (RFP) was originally isolated from Discosoma sp., a sea anemone. It is a naturally fluorescent protein which emits red light at a maximum wavelength of 583 nm in response to a specific light wavelength, maximized at 558 nm. It is used in laboratory as a reporter molecule to label and to study protein-protein interactions and protein localization.
Product Type:
Antibody
Antibody Type:
Monoclonal (clone RF5R)
Format:
Liquid
Storage Temp:
Antibody can be stored at -20°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
Red fluorescent protein (RFP)
Immunogen:
dsRed, recombinant Red Fluorescent Protein expressed in bacteria
Applications:
Western Blot (WB), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Antibody is purified on Protein A.Sensitivity: WB (with ECL): 1-10 ng of purified RFP or RFP fusion proteins. Primary antibody should be incubated for one hour at room temperature.
Clone number:
IgG1
Application Details:
1 : 1000-3000 (WB)
Purity:
Total IgG1. Protein A purified in 1mg/mL in 10mM PBS, pH 7.2. Contains 0.05% sodium azide.
Related products:
Secondary antibodies
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Cecchini et al. (2020). Kinases and protein motifs required for AZI1 plastid localization and trafficking during plant defense induction. Plant J. 2020 Dec 20. doi: 10.1111/tpj.15137. Epub ahead of print. PMID: 33342031.
Special application note:
Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally
V5-tag is a tag that can be added to a protein of interest as a fusion protein to enable purification and detection.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Antibody is purified on Protein A.For western blot incubate antibody for one hour at room temperature.
Clone number:
IgG1
Application Details:
1 : 500-1 :2000 (IHC) , 1 : 1000-1: 3000 (WB)
Purity:
Total IgG. Protein A purified from culture supernatant of hybridoma cells grown in a bioreactor. Provided in 10 mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
Reconstitution:
For reconstitution add 50 µl of sterile water
Related products:
Secondary antibodies
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. GFP protein has molecular weight of 27 kDa.Source of GFP standard: Wild type recombinant GFP from A. victoria was expressed in E. coli.
Product Type:
Antibody
Format:
Liquid
Storage Temp:
Store in undiluted aliquots at -20°C; to avoid repeated freeze-thaw cycles. Store up to 24 months.
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa. This antibody is directly conjugated to soyabean peroxidase.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C or in small aliquotes at -20°C; avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Native GFP, Recombinant GFP (E,coli), all variants of GFP
Immunogen:
highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212
Applications:
ELISA (ELISA), Immunogold (IG), Immunohistochemistry (IHC), Western blot (WB)
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Native GFP, Recombinant GFP (E.coli), all variants of GFP and EGFP
Immunogen:
highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212
Wieczorek et al. (2020) Development of a New Tomato Torrado Virus-Based Vector Tagged with GFP for Monitoring Virus Movement in Plants. Viruses. 2020 Oct 20;12(10):1195. doi: 10.3390/v12101195. PMID: 33092281; PMCID: PMC7588970.
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Native GFP, Recombinant GFP (E.coli), variants of GFP
Immunogen:
Highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212
Applications:
ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C; make aliquots to avoid working with a stock. Or Store in small aliquots at -20°C, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
Native GFP, Recombinant GFP (E,coli), all variants of GFP
Immunogen:
highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at -20°C. Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Native GFP, Recombinant GFP (E,coli), all variants of GFP, including EGFP
Immunogen:
Highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
Sun et al. (2021) The epigenetic factor FVE orchestrates cytoplasmic SGS3-DRB4-DCL4 activities to promote transgene silencing in Arabidopsis. Sci Adv. 2021 Aug 4;7(32):eabf3898. doi: 10.1126/sciadv.abf3898. PMID: 34348894; PMCID: PMC8336953.Stelate et al. (2021) Correlative Light-Environmental Scanning Electron Microscopy of Plasma Membrane Efflux Carriers of Plant Hormone Auxin. Biomolecules. 2021 Sep 26;11(10):1407. doi: 10.3390/biom11101407. PMID: 34680040; PMCID: PMC8533460.Wieczorek et al. (2020) Development of a New Tomato Torrado Virus-Based Vector Tagged with GFP for Monitoring Virus Movement in Plants. Viruses. 2020 Oct 20;12(10):1195. doi: 10.3390/v12101195. PMID: 33092281; PMCID: PMC7588970.Kulich et al. (2018). Deubiquitinase OTU5 affects Root Responses to Phosphate Starvation. Plant Physiol. 2018 Jan 4. pii: pp.01693.2017. doi: 10.1104/pp.17.01693.
Special application note:
10 mM TRIS buffer pH 8,0 containing 0,02% sodium azide
Rabbit anti-T7 is a primary antibody which binds to T7 and is directly conjugated to DyLight® 550. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Rabbit anti-T7 is a primary antibody which binds to T7 and is directly conjugated to DyLight® 488. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Rabbit anti-T7 is a primary antibody which binds to T7 and is directly conjugated to SureLight® R-Phycoerythrin (R-PE).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Rabbit anti-T7 is a primary antibody which binds to T7 and is directly conjugated to DyLight® 650. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Rabbit anti-T7 is a primary antibody which binds to T7 and is directly conjugated to Biotin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Rabbit anti-T7 is a primary antibody which binds to T7 and is directly conjugated to ALP, Alkaline Phosphatase. This is of advantage to shorten assay time by no need to use a secondary antibody to T7.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Rabbit anti-S is a primary antibody which binds to S and is directly conjugated to Biotin. This is of advantage to shorten assay time by no need to use a secondary antibody to S.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Rabbit anti-S is a primary antibody which binds to S and is directly conjugated to ALP, Alkaline Phosphatase. This is of advantage to shorten assay time by no need to use a secondary antibody to S.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Rabbit anti-S is a primary antibody which binds to S and is directly conjugated to DyLight® 550. This is of advantage to shorten assay time by no need to use a secondary antibody to S. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Rabbit anti-S is a primary antibody which binds to S and is directly conjugated to DyLight® 488. This is of advantage to shorten assay time by no need to use a secondary antibody to S. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Rabbit anti-S is a primary antibody which binds to S and is directly conjugated to SureLight® R-Phycoerythrin (R-PE). This is of advantage to shorten assay time by no need to use a secondary antibody to S.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Rabbit anti-S is a primary antibody which binds to S and is directly conjugated to DyLight® 650. This is of advantage to shorten assay time by no need to use a secondary antibody to S. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Rabbit anti-V5 is a primary antibody which binds to V5 and is directly conjugated to SureLight® R-Phycoerythrin (R-PE).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Rabbit anti-V5 is a primary antibody which binds to V5 and is directly conjugated to DyLight® 650. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Rabbit anti-V5 is a primary antibody which binds to V5 and is directly conjugated to DyLight® 550. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Rabbit anti-V5 is a primary antibody which binds to V5 and is directly conjugated to DyLight® 488. This is of advantage to shorten assay time by no need to use a secondary antibody to V5. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Rabbit anti-V5 is a primary antibody which binds to V5 and is directly conjugated to ALP, Alkaline Phosphatase.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Rabbit anti-HA is a primary antibody which binds to HA and is directly conjugated to DyLight® 650. This is of advantage to shorten assay time by no need to use a secondary antibody to HA. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Rabbit anti-HA is a primary antibody which binds to HA and is directly conjugated to DyLight® 550. This is of advantage to shorten assay time by no need to use a secondary antibody to HA. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Rabbit anti-HA is a primary antibody which binds to HA and is directly conjugated to DyLight® 488. This is of advantage to shorten assay time by no need to use a secondary antibody to HA. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Rabbit anti-HA is a primary antibody which binds to HA and is directly conjugated to SureLight® R-Phycoerythrin. This is of advantage to shorten assay time by no need to use a secondary antibody to HA.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®) and is directly conjugated to DyLight® 488. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Shelf life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (binds to Sigma FLAG®) and is directly conjugated to SureLight® R-Phycoerythrin.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®) and is directly conjugated to DyLight® 650. This is of advantage to shorten assay time by no need to use a secondary antibody.DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Mouse anti-c-myc is a primary antibody which binds to c-myc tag and is directly conjugated to DyLight® 550. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Host Animal:
Mouse
Immunogen:
Epitope EQKLISEEDL sequence from the human c-myc protein
Mouse anti-c-myc is a primary antibody which binds to c-myc tag and is directly conjugated to DyLight® 488. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Host Animal:
Mouse
Immunogen:
Epitope EQKLISEEDL sequence from the human c-myc protein
Mouse anti-c-myc is a primary antibody which binds to c-myc tag and is directly conjugated to SureLight® R-Phycoerythrin.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Host Animal:
Mouse
Immunogen:
Epitope EQKLISEEDL sequence from the human c-myc protein
Mouse anti-c-myc is a primary antibody which binds to c-myc tag which is directly conjugated to DyLight® 650. This is of advantage to shorten assay time. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Host Animal:
Mouse
Immunogen:
Epitope EQKLISEEDL sequence from the human c-myc protein
Mouse anti-c-myc is a primary antibody which binds to c-myc and is directly conjugated to ALP, Alkaline Phosphatase. This is of advantage to shorten assay time by no need to use a secondary antibody.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Host Animal:
Mouse
Immunogen:
Epitope EQKLISEEDL sequence from the human c-myc protein
His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine and is directly conjugated to ALP, Alkaline Phosphatase. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified in 25 mM Tris, 150 mM NaCl, 5 mM MgCl2, 0.12 mM ZnCl2, pH 7.4. with 2 mM sodium azide.
Reconstitution:
For reconstitution add 100 µl of sterile water
Special application note:
Antibody is provided in: 25 mM Tris, 150 mM Sodium Chloride, 5 mM Magnesium Chloride, 0.12 mM Zinc Chloride, 2 mM Sodium Azide (pH 7.4)Concentration: 0.1mg/ml
Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine tag and is directly conjugated to Biotin. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine tag and is directly conjugated to DyLight®650. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Host Animal:
Mouse
Immunogen:
Polyhistidine (HHHHHH) tagged polypeptide
Applications:
High throughput screening (HTS), Immunofluorescence (IF), Immunohistochemisty (IHC), Western blot (WB)
Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine tag and is directly conjugated to Europium. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine tag and is directly conjugated to SureLight® R-Phycoerythrin. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine tag and is directly conjugated to HRP. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag.
Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine tag and is directly conjugated to DyLight® 550. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine tag and is directly conjugated to DyLight® 488. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Mouse anti-HA is a primary antibody which binds to HA and is directly conjugated toDyLight® 650. This is of advantage to shorten assay time by no need to use a secondary antibody to HA. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Host Animal:
Mouse
Immunogen:
HA-tag:YPYDVPDYA
Applications:
Flow cytometry (Flow cyt) and Cell Based Assays (CBA)(CBA)
Mouse anti-HA is a primary antibody which binds to HA and is directly conjugated to HRP. This is of advantage to shorten assay time by no need to use a secondary antibody to HA.
Mouse anti-HA is a primary antibody which binds to HA and is directly conjugated to biotin. This is of advantage to shorten assay time since there is no need to use a secondary antibody to HA.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Mouse anti-HA is a primary antibody which binds to HA and is directly conjugated to Alkaline Phosphatase . This is of advantage to shorten assay time when no need to use a secondary antibody to HA.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
The optimal working dilution should be determined by the investigator
Purity:
Immunogen affinity purified in 25 mM Tris, 150 mM NaCl, 5 mM MgCl2, 0.12 mM ZnCl2, pH 7.4. with 2 mM sodium azide.
Selected references:
Wang et al. (2017). The inhibition of protein translation mediated by AtGCN1 is essential for cold tolerance in Arabidopsis thaliana. Plant Cell Environ. 2017 Jan;40(1):56-68. doi: 10.1111/pce.12826.
Special application note:
Antibody is provided in: 25 mM Tris, 150 mM Sodium Chloride, 5 mM Magnesium Chloride, 0,12 mM Zinc Chloride, pH 7,4 with 2 mM Sodium Azide
Mouse anti-HA is a primary antibody which binds to HA and is directly conjugated toDyLight®550. This is of advantage to shorten assay time by no need to use a secondary antibody to HA. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Mouse anti-HA is a primary antibody which binds to HA and is directly conjugated toDyLight®488. This is of advantage to shorten assay time by no need to use a secondary antibody to HA. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Mouse anti-HA is a primary antibody which binds to HA and is directly conjugated to SureLight® R-Phycoerythrin. This is of advantage to shorten assay time by no need to use a secondary antibody to HA.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Host Animal:
Mouse
Immunogen:
HA-tag: YPYDVPDYA
Applications:
Flow Cytometry (FL), Cell Based Assays (CBA)(CBA), Microarrays (MA), and Microplate applications (MPl)
Goat anti-GST is a primary antibody which binds to GST which is directly conjugated to DyLight® 550. This is of advantage to shorten assay time by no need to use a secondary antibody to GST. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Goat anti-GST is a primary antibody which binds to GST and is directly conjugated to DyLight® 488. This is of advantage to shorten assay time by no need to use a secondary antibody to GST. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 2-8°C. The shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Goat anti-GST is a primary antibody to GST directly conjugated to SureLight® R-Phycoerythrin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
Host Animal:
Goat
Immunogen:
Glutathione-S-transferase from S. japonicum
Applications:
Flow cytometry (Flow cyt), Cell Based Assays (CBA)(CBA), Microarrays (MA), and Microplate applications (MPl)
Goat anti-GST IgG is a primary antibody which binds to GST and is directly conjugated to DyLight® 650. This is of advantage to shorten assay time by no need to use a secondary antibody to GST. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store at 2-8°C.; Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
FLAG-tag is a tag that can be added to a protein sequence motif DYKXXD. It can be used for affinity chromatography, to separate recombinant protein from wt proteins. It can also be used to isolate protein complexes with multiple subunits.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C for up to 2 years.
Host Animal:
Mouse
Species Reactivity:
N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins
Immunogen:
KLH-conjugated DYKDDDDK (FLAG) synthetic peptide
Applications:
Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
1 : 1000-5000 (1-5ng) (WB) (incubate for one hour at room temperature), 1 : 500-1 :2000 (IS) For best results with other assays (Dot, ELISA, IP), please determine optimal working dilution by end user
Purity:
Total IgG. Protein A purified in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
Related products:
Plant and algal protein extraction buffer Secondary antibodies
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Special application note:
Working dilution for Dot Blot, ELISA and IP needs to be determined experimentally.Antibody is purified on Protein A.
Digoxigenin (DIG) is an hapten from plants (Digitalis) and can be used as an epitope tag in many biological applications.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Lyophilized powder is stable for a minimum of 2 years at -20°C. Reconstitute in distilled water before use. Store reconstituted antibodies at 4°C for up to a month and make aliquots for extended storage.
Host Animal:
Mouse
Species Reactivity:
Antibody detects free and bound digoxigenin tag
Immunogen:
Digoxigenin
Applications:
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Strep-tag®II is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys coded also as WSHPQFEKGS) and can be N- or C- terminally fused to recombinant proteins.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid in PBS at 0.5 mg/ml.
Storage Temp:
Store at 4°C for 1-2 weeks (short term). For log term storage, aliquot and store at -20°C and below, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Host Animal:
Mouse
Species Reactivity:
Artificial sequence (3x WSHPQFEKGS)
Immunogen:
Recombinant human protein containing three tandem repeated Strep II tags (3xStrep-tag), separated by GlySer-linker sequence, at the N-terminus (expressed in HEK293 cells).
VSV-G tag corresponds to the partial peptide sequence of the vesicular stomatitis virus glycoprotein from Rhabdoviridae family, and consists of a single RNA molecule that encodes five proteins, one of which is a surface glycoprotein (G).. This epitope tag can be added to a protein from any species to allow further protein detection using different techniques like: immunolocalization, immunoprecipitation or Western blot.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid in PBS
Storage Temp:
Store at -20°C; Avoid repeated freeze/thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes. For long term storage, transfer to -80°C
TAP (tandem-affinity-purification) epitope tag makes a rapid purification of low abundance complexes. TAP approach can be combined with mass spectrometry to allow identification of protein interactions with a given target protein.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
TAP epitope tag
Immunogen:
TAP epitope tag, sequence: CSSGALDYDIPTTASENLYFQ, derived from the C-terminus of the TAP-tag construct after TEV cleavage,
CBP tag comes from muscle myosin light-chain kinase and contains 26 amino acid residues with the molecular weight of 4 kDa. This tag is characterized by the relatively high affinity for calmodulin (CaM), which makes it possible to purify CBP-tagged proteins from crude cell extracts using of a resin with CaM affinity. This antibody allows detection of CBP-tagged proteins.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
The anti-Myc tag is a primary antibody which is used to detect proteins containing the Myc epitope tag. The Myc tag contains the amino acid sequence EQKLISEEDL, corresponding to amino acids 410-419 of human Myc.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
S-tag from pancreatic ribonuclease A (RNase A). Due to abundance of charged and polar residues, this tag may improve solubility of recombinant proteins. It can be fused at the N- or C-terminus of a target protein.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Trx (Thioredoxin 1) is a redox protein with a primary domain conserved across a number of Trx family members. The protein contains a conserved catalytic site Cys-Gly-Pro-Cys. The protein is used as a fusion tag in a number of molecular biology applications.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
KT3 epitope tag consists of 11 amino acids in the leader sequence of T7 bacteriophage gene10 and is used as a tag in many expression vectors including pET system. Specific anti-T7 tag antibodies are suitable for detection of T7-tagged proteins.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
T7 epitope tag consists of 11 amino acids in the leader sequence of T7 bacteriophage gene10 and is used as a tag in many expression vectors including pET system. Specific anti-T7 tag antibodies are suitable for detection of T7-tagged proteins.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
MBP (Maltose binding protein) is encoded by the malE gene of E.coli and is a commonly used tag when studying protein expression using a wide range of applications. MBP tag enables easy purification of proteins from bacterial extracts under mild conditions.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C for up to 1 year, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C for up to 3 years, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Immunogen:
GST (glutathione S-transferase) recombinant protein
V5-tag is a tag that can be added to a protein of interest as a fusion protein to enable purification and detection. It is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). Addition of a V5-Tag to a protein of interest makes it possible to localize a specific gene product in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
V5-tagged fusion proteins
Immunogen:
KLH-conjugated GKPIPNPLLGLDST synthetic peptide
Applications:
ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
c-Myc is derived from Myc proto-oncogene protein. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. A short peptide was used as an epitope tag, that is easily recognized by tag specific antibodies. This is a universal detection reagent which will allow detection of any c-Myc tag containing protein.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid at 1 mg/ml
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
c-Myc tagged fusion proteins
Immunogen:
Conjugated synthetic peptide: AEEQKLISEEDLL derived from the C-terminal region of human c-Myc. UniProt: P01106
c-Myc is derived from Myc proto-oncogene protein. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. A short peptide was used as an epitope tag, that is easily recognized by tag specific antibodies. This is a universal detection reagent which will allow detection of any c-Myc tag containing protein.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid at 1 mg/ml
Storage Temp:
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
c-Myc tagged fusion proteins
Immunogen:
Conjugated synthetic peptide: AEEQKLISEEDLL derived from the C-terminal region of human c-Myc. UniProt: P01106
c-Myc is derived from Myc proto-oncogene protein. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. A short peptide was used as an epitope tag, that is easily recognized by tag specific antibodies. This is a universal detection reagent which will allow detection of any c-Myc tag containing protein.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid at 1 mg/ml
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
c-Myc tagged fusion proteins
Immunogen:
Conjugated synthetic peptide: AEEQKLISEEDLL derived from the C-terminal region of human c-Myc. UniProt: P01106
Applications:
Immunoprecipitation (IP), Immunohistochemisty (IHC), paraffin, Flow cytometry (Flowcyt), Western blot (WB)
Dendra2 is an improved version of green-to-red photo switchable protein Dendra, from an octocoral (Dendronephthya) and compared to it, Dendra2 exhibits brighter fluorescence before and after photoswitching. Excitation maximum of Dendra2 is 490 nm before and 553 nm after photoactivation, and its emission maximum is 507 nm before and 573 nm after photoactivation. Activating light for Dendra2 is UV/violet to blue. Nonactivated Dendra2 spectral characteristics are similar to EGFP, and this green fluorescence can be detected at low light intensities of blue light. At high intensities of the same blue light (or of UV/violet light) Dendra2 is photoactivated and gets emission characteristics similar to TRITC.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid at 1 mg/ml
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Dendra2-tagged proteins
Immunogen:
Dendra2 tag protein
Applications:
Immunocytochemistry (ICC), Flow cytometry (FlowCyt) (QC tested), Western blot (WB)
GST-tag (glutathione S-transferase) from a parasite Schistosoma japonicum is a tag added to a protein of interest as a fusion protein for protein purification and detection. It allows purification by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid at 1 mg/ml
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
To be added when available, antibody released in October 2021.
Special application note:
This antibody is recognizing native and denatured fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems. It can be also used for immuno purification of GST-tagged proteins.
c-Myc is derived from Myc proto-oncogene protein. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. A short peptide was used as an epitope tag, that is easily recognized by tag specific antibodies. This is a universal detection reagent which will allow detection of any c-Myc tag containing protein.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid at 1 mg/ml
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
c-Myc tagged fusion proteins
Immunogen:
Conjugated synthetic peptide: AEEQKLISEEDLL derived from the C-terminal region of human c-Myc. UniProt: P01106
Applications:
Flow cytometry (Flow cyt), Immunohistochemistry (IHC), paraffin, Immunoprecipitation (IP), Western blot (WB)
Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®). The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid at 1 mg/ml
Storage Temp:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
EGFP is an Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), and oxidation of Tyr-(N+1) to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lypholized antibody at 4 ºC. After reconstitution keep aliquots at -20ºC for a higher stability. Avoid repetitive freeze/thaw cycles. Centrifuge briefly to remove any insoluble material.Expiry date: 12 months after purchase if unopened
Host Animal:
Rabbit
Species Reactivity:
GFP, EGFP
Immunogen:
Recombinant GFP from Aequorea coerulescens (belt jellyfish) overexpressed and purified from E.coli, UniProt: Q6YGZ0
mCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, hoever it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Mouse
Species Reactivity:
mCherry
Immunogen:
Recombinant full length mCherry expressed and purified from E. coli.
LUC (Luciferase) from Photinus pyralis (Common eastern firefly) (Lampyris pyralis), light producing beetle is an enzyme in the light production.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
LUC
Immunogen:
KLH-conjugated synthetic peptide derived from N-terminal part of LUC protein sequence of Photinus pyralis, UniProt: Q27758
Plant and algal protein extraction buffer Secondary antibodies
Molecular Weight:
Depends upon a MW of a protein which is LUC-tagged,
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Yuan et al. (2021). BBX19 fine-tunes the circadian rhythm by interacting with PSEUDO-RESPONSE REGULATOR proteins to facilitate their repressive effect on morning-phased clock genes, The Plant Cell, 2021;, koab133, https://doi.org/10.1093/plcell/koab133
LUC (Luciferase) from Photinus pyralis (Common eastern firefly) (Lampyris pyralis), light producing beetle is an enzyme in the light production.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
LUC
Immunogen:
KLH-conjugated synthetic peptide derived from N-terminal part of LUC protein sequence of Photinus pyralis, UniProt: Q27758
Plant and algal protein extraction buffer Secondary antibodies
Molecular Weight:
Depends upon a MW of a protein which is LUC-tagged,
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Ormancey et al. (2023) Complementary peptides represent a credible alternative to agrochemicals by activating translation of targeted proteins. Nat Commun. 2023;14(1):254. Published 2023 Jan 17. doi:10.1038/s41467-023-35951-0
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.This antibody is directly conjugated to HRP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
Staphylococcus aureus
Expected Species:
Staphylococcus pyogenes UniProt: Q99ZW2
Immunogen:
KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6(peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.This antibody is directly conjugated to Biotin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
Staphylococcus aureus
Expected Species:
Staphylococcus pyogenes UniProt: Q99ZW2
Immunogen:
KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6(peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.This antibody is directly conjugated to ALP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
Staphylococcus aureus
Expected Species:
Staphylococcus pyogenes UniProt: Q99ZW2
Immunogen:
KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6(peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
Staphylococcus aureus
Expected Species:
Staphylococcus pyogenes UniProt: Q99ZW2
Immunogen:
KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6(peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
GUS (Beta-glucuronidase) is an enzyme which when incubated with colorless or non-fluorescent substrates, can catalyze reaction of their transformation into coloured or fluorescent products.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
GUS
Immunogen:
KLH-conjugated synthetic peptide derived from Escherichia coli GUS protein at amino acid 358.
Plant and algal protein extraction buffer Secondary antibodies
Molecular Weight:
depends upon a MW of a protein which is expressed with GUS
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Xiumei et al. (2022) Pathogenesis-related protein 1 suppresses oomycete pathogen by targeting against AMPK kinase complex,Journal of Advanced Research, 2022, ISSN 2090-1232, https://doi.org/10.1016/j.jare.2022.02.002.Xiao et al. (2022) An amino acid transporter-like protein (OsATL15) facilitates the systematic distribution of thiamethoxam in rice for controlling the brown planthopper. Plant Biotechnol J. 2022 Jun 9. doi: 10.1111/pbi.13869. Epub ahead of print. PMID: 35678495. (Immunolocalization)Nizkorodova et al. (2020). The Effect of Translation Promoting Site (TPS) on Protein Expression in E. coli Cells. Mol Biotechnol (2020). doi.org/10.1007/s12033-020-00251-1Nikorodova and Isakov (2019). New insights into the mechanism of action of e-element enhancer in E.coli. Eurasian Journal of Applied Biotechnology 2/2019
Special application note:
This antibody will not work with pCAMBIA vectors due to mutation starting at amino acid position 358
HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Important note: there is a strong cross reactivity between 25 -20 kDa in Arabidopsis thaliana wildtype.
Application Details:
1 : 5000 (WB)
Purity:
affinity purified serum in PBS, pH 7.4.
Reconstitution:
For reconstitution add 100 µl of sterile water
Related products:
Plant and algal protein extraction buffer Secondary antibodies
Molecular Weight:
Depends on the MW of the protein which is HA-tagged
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Hwang et al. (2019) Arabidopsis ABF3 and ABF4 Transcription Factors Act with the NF-YC Complex to Regulate SOC1 Expression and Mediate Drought-Accelerated Flowering. Mol Plant. 2019 Apr 1;12(4):489-505. doi: 10.1016/j.molp.2019.01.002
Strep-tag®II is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) and this peptide sequence shows high affinity towards Strep-Tactin®, a specifically engineered streptavidin, and can be N- or C- terminally fused to recombinant proteins.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
HSV (herpes simplex virus) eptiope tag originates from envelope glycoprotein D. It is frequently used to target N- or C- terminus of a protein of interest to allow target protein detection, in case when specific antibodies are not available.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
YFP (Yellow Fluorescent Protein) is a genetic mutant of green fluorescent protein (GFP). YFP has an excitation peak at 514 nm and emission peak at 527 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
N-YFP tagged proteins from Arabidopsis thaliana, Escherichia coli, Nicotiana tabacum
Immunogen:
KLH-conjugated synthetic peptide derived from N-terminal of YFP protein.
YFP molecular weight is 27 kDa, however detected band is going to have MW increased by a fused protein
Application Details:
1 : 2000 (WB)
Purity:
Serum
Reconstitution:
For reconstitution add 50 µl of sterile water
Related products:
Plant and algal protein extraction buffer Secondary antibodies
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Li et al. (2021) Two ubiquitin-associated ER proteins interact with COPT copper transporters and modulate their accumulation, Plant Physiology, 2021;, kiab381, https://doi.org/10.1093/plphys/kiab381Lung et al. (2021) Oxylipin signaling in salt-stressed soybean is modulated by ligand-dependent interaction of Class II acyl-CoA-binding proteins with lipoxygenase. Plant Cell. 2021 Dec 17:koab306. doi: 10.1093/plcell/koab306. Epub ahead of print. PMID: 34919703.Schultz-Larsen et al. (2018). The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity. Cell Rep. 2018 Nov 27;25(9):2329-2338.e5. doi: 10.1016/j.celrep.2018.11.011.
YFP (Yellow Fluorescent Protein) is a genetic mutant of green fluorescent protein (GFP). YFP has an excitation peak at 514 nm and emission peak at 527 nm.This antibody is directly conjugated to HRP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Avoid sodium azide.
Host Animal:
Rabbit
Species Reactivity:
C-YFP tagged proteins from Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from C-terminal of YFP protein. This peptide is conserved in pGWB541 Vector.
YFP (Yellow Fluorescent Protein) is a genetic mutant of green fluorescent protein (GFP). YFP has an excitation peak at 514 nm and emission peak at 527 nm.This antibody is directly conjugated to Biotin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
C-YFP tagged proteins from Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from C-terminal of YFP protein. This peptide is conserved in pGWB541 Vector.
YFP (Yellow Fluorescent Protein) is a genetic mutant of green fluorescent protein (GFP). YFP has an excitation peak at 514 nm and emission peak at 527 nm.This antibody is directly conjugated to ALP.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid
Storage Temp:
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Host Animal:
Rabbit
Species Reactivity:
C-YFP tagged proteins from Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from C-terminal of YFP protein. This peptide is conserved in pGWB541 Vector.
YFP (Yellow Fluorescent Protein) is a genetic mutant of green fluorescent protein (GFP). YFP has an excitation peak at 514 nm and emission peak at 527 nm.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Rabbit
Species Reactivity:
C-YFP tagged proteins from Arabidopsis thaliana
Immunogen:
KLH-conjugated synthetic peptide derived from C-terminal of YFP protein. This peptide is conserved in pGWB541 Vector.
Plant and algal protein extraction buffer Secondary antibodies
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
Li et al. (2021) Two ubiquitin-associated ER proteins interact with COPT copper transporters and modulate their accumulation, Plant Physiology, 2021;, kiab381, https://doi.org/10.1093/plphys/kiab381Lung et al. (2021) Oxylipin signaling in salt-stressed soybean is modulated by ligand-dependent interaction of Class II acyl-CoA-binding proteins with lipoxygenase. Plant Cell. 2021 Dec 17:koab306. doi: 10.1093/plcell/koab306. Epub ahead of print. PMID: 34919703.
His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
Store at -20°C.
Host Animal:
Mouse
Species Reactivity:
6xHis
Expected Species:
6xHis
Immunogen:
KLH-conjugated synthetic peptide 6xHis
Applications:
Dot Blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunlocalization (IL), Western blot (WB)
Antibody is present in 10 mM PBS, pH 7.2His-tag (6,8,10xHis) needs to be properly exposed to allow detection. To prevent target protein folding, extraction should be performed with 6 to 8 M urea or using TCA-acetone precipitation method.
Clone number:
IgG2b
Application Details:
1 : 1000 (WB)
Purity:
Total IgG fraction. Protein A purified.
Related products:
Plant and algal protein extraction buffer Secondary antibodies
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
Selected references:
De Brasi-Velasco et al. (2021). Autophagy Is Involved in the Viability of Overexpressing Thioredoxin o1 Tobacco BY-2 Cells under Oxidative Conditions. Antioxidants. 2021; 10(12):1884. https://doi.org/10.3390/antiox10121884Tan et al. (2020). Salicylic Acid Targets Protein Phosphatase 2A to Attenuate Growth in Plants. Curr Biol. 2020 Feb 3;30(3):381-395.e8. doi: 10.1016/j.cub.2019.11.058.López-Vidal et al. (2020). Is Autophagy Involved in Pepper Fruit Ripening? Cells, 9 (1) , DOI: 10.3390/cells9010106 Häggmark-Månberg et al. (2016). Autoantibody targets in vaccine-associated narcolepsy. Autoimmunity. 2016 Sep;49(6):421-433. Epub 2016 May 20.
Special application note:
Working dilution for ELISA, IL and IP needs to be determined experimentally
c-Myc is derived from Myc proto-oncogene protein. A short peptide located between amino acids 408-420 is used as an epitope tag, that is easily recognized by tag specific antibodies. This is a universal detection reagent which will allow detection of any tag containing protein.This antibody is directly conjugated to Biotin.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
Store lyophilized antibodies at -20°C and reconstituted antibodies at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal:
Chicken
Species Reactivity:
c-Myc
Expected Species:
c-Myc
Immunogen:
KLH-conjugated peptide, sequence MEQKLISEEDLNE, human c-Myc UniProt: Q6LBK7
Depends on target protein which is tagged with this epitope
Not reactive in:
No confirmed exceptions from predicted reactivity are currently known.
UniProt number:
P01106
Gene ID:
4609
Subcategory:
c-Myc
Research area:
Ag-Tag Antibodies
Code:
Ag-Tag15
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