Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released. This antibody is raised in sheep to detect the prodomain of NGF not the mature peptide.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
The recombinant prodomain fragment of human nerve growth factor
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, Immunofluorescence, ELISA, Western Blot, biological neutralization of proNGF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
pro-brain nerve growth factor; proNGF; NGF;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Srinivasan B et al. J Biol Chem. 2004 Oct 1;279(40):41839-4 Yune TY et al. J Neurosci. 2007 Jul 18;27(29):7751-61.
Specificity:
The specificity of this antibody has been confirmed by WB. It does NOT crossreact with proBDNF, proNT-3 or mature NGF. Confirmed to react with purified human proNGF and crossreact with mouse and rat proNGF
Brain derived neurotrophic factor (BDNF) is synthesized as a precursor (proBDNF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proBDNF is synthesized in neurons and glia (eg., microglia), transported anterogradely and retrogradely and may be released in an activity dependent manner. This antibody is raised in sheep to detect the prodomain of BDNF and not the mature peptide.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
The recombinant prodomain fragment of human brain-derived neurotrophic factor
Applications:
ELISA,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
ELISA, Western Blot, biological neutralization of proBDNF, Immunocytochemistry/Immunofluorescence. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Proform brain derived neurotrophic factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Fan, YJ. et al http://www.ncbi.nlm.nih.gov/pubmed/18430030?ordinalpos=6&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum'> (2008) Eur J Neurosci 27(9):2380-90. Ulman, L. et al http://www.ncbi.nlm.nih.gov/pubmed/18971468'> (2008) J Neurosci. Oct 29;28(44):11263-8.
Specificity:
Confirmed to react with purified human proBDNF, crossreact with mouse and rat proBDNF Cross reactivity with other species than human, mouse and rat has not yet been tested
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Ebendal, T. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2468788'> (1989) J Neurosci Res 22, 223-240. Zhou, X. F. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7815824'> (1994) J Neurosci Methods 54, 95-102. Angeletti, P. U. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=4880217'> (1968) Adv Enzymol Relat Areas Mol Biol 31, 51-7 Hesse K. et al. http://www.ncbi.nlm.nih.gov/pubmed/9291146'> (1997) Neurosci Lett. Aug 8;231(2):83- Miao J et al. http://www.ncbi.nlm.nih.gov/pubmed/23000062'> (2012) Neurosci Res. Dec;74(3-4):269-7
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Ebendal, T. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2468788'> (1989) J Neurosci Res 22, 223-240. Zhou, X. F. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7815824'> (1994) J Neurosci Methods 54, 95-102. Angeletti, P. U. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=4880217'> (1968) Adv Enzymol Relat Areas Mol Biol 31, 51-7 Hesse K. et al. http://www.ncbi.nlm.nih.gov/pubmed/9291146'> (1997) Neurosci Lett. Aug 8;231(2):83- Miao J et al. http://www.ncbi.nlm.nih.gov/pubmed/23000062'> (2012) Neurosci Res. Dec;74(3-4):269-7
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A dilution of 1:1000-1:5000 is recommended for IHC, western blot and immunoblot; 1:15000 for ELISA; for inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Ebendal, T. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2468788'> (1989) J Neurosci Res 22, 223-240. Zhou, X. F. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation_uids=7815824'> (1994) J Neurosci Methods 54, 95-102. Angeletti, P. U. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=4880217'> (1968) Adv Enzymol Relat Areas Mol Biol 31, 51-7 Hesse K. et al. http://www.ncbi.nlm.nih.gov/pubmed/9291146'> (1997) Neurosci Lett. Aug 8;231(2):83- Miao J et al. http://www.ncbi.nlm.nih.gov/pubmed/23000062'> (2012) Neurosci Res. Dec;74(3-4):269-7
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
Sheep anti-Myelin basic protein (MBP) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of approximately 80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP). MBP is an 'intrinsically unstructured' protein with a high proportion (approximately 75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system. A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational. Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic. The message for myelin basic protein is selectively translocated to the ends of the cell processes. Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model. FUNCTION: Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity). SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Myelin membrane; peripheral membrane protein; cytoplasmic side. Cytoplasmic side of myelin. TISSUE SPECIFICITY: Found in both the central and the peripheral nervous system. PTM: At least 5 charge isomers; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4 and C5 (the least cationic form); are produced as a result of optional posttranslational modifications such as phosphorylation of serine or threonine residues, deamidation of glutamine or asparagine residues, citrullination and methylation of arginine residues. C1 and C2 are unphosphorylated, C3 and C4 are monophosphorylated and C5 is phosphorylated at two positions. SIMILARITY: Belongs to the myelin basic protein family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Guinea Pig,Human,Rat
Immunogen:
A synthetic peptide (YG SLPQKSQRSQ DENPVV, aa: 68-86) as part of guinea pig MBP protein conjugated to KLH
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1:4000 is recommended. Immunostaining for MBP of abnormal appearing oligodendrocytic process and cell bodies in demyelinating areas. This antibody recognises only areas of myelin degeneration when tested in injured spinal cord and lesioned sciatic nerves. It also stains discrete white matter in the brain of multiple system atrophy. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Myelin Basic Protein
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Schwartz, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12440382'>Prog Brain Res 137, 401-6 (2002) Hauben, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve=PubMed&dopt=Citation&list_uids=11518733'>J Clin Invest 108, 591-9 (Aug, 2001) Yoles, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11356861'>J Neurosci 21, 3740-8 (Jun 1, 2001) Hauben, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10964948'>J Neurosci 20, 6421-30 (Sep 1, 2000) Harauz, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15219899'>Nature 389, 783-4 (1997). Micron 35, 503-42 (2004) Givogri, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10650873'>J Neurosci Res 59, 153-9 (Jan 15, 2000) Kim, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9251242'>Int J Biochem Cell Biol 29, 743-51 (May, 1997) Kalwy, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7522797'>Mol Membr Biol 11, 67-78 (Apr-Jun, 1994) Wajgt, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6198864'>Acta Neurol Scand 68, 337-43 (Nov, 1983) Day, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2418054'>J Neuroimmunol 10, 289-312 (Feb, 1986) 1Mikoshiba, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1709079'>Comp Biochem Physiol C 98, 51-61 (1991) 1Brophy, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7509522'>Trends Neurosci 16, 515-21 (Dec, 1993) 1Matsuo, A. et al. (1997) Am. J. Pathol. 150(4): 1253-1266
Specificity:
This antiserum recognizes MBP in demyelinated nerve tissues. Immunohistochemical analysis of lesioned rat spinal cord indictaes a high level of specificity for this antiserum. This antiserum reacts with human and rat MBP.
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human GDNF
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB. Recommended to be used at a dilution of 1:1000 to 1:3000 for Western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human GDNF
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC, WB. A dilution of 1 µg/mL is recommended for both applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human BDNF
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, Inhibition of biological activity in vitro/in vivo. Recommended to be used at a dilution of 1:200-2000 for immunohistochemistry on Zamboni's fixed frozen tissue; not recommended for formalin fixed paraffin embedded tissues. 1:10 to 1:50 for inhibition of biological activity in vitro. Use neat for in vivo studies at 5-10 µL/g body weight. Whole serum format will caused immune responses, purified format is preferred for most in vivo work. Not recommended for western blots. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A Acheson et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7700353'>(1995) Nat. 74: 450-3 Q Yan et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8083736'>(1994) J. Neurosci. 14(9): 5281-91 XF Zhou et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8895863'>(1996) Neurosci. 74: 945-53 XF Zhou, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9454633'>(1998) Exp. Neurol. 149: 237-42 B Mellstrom et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15581398'>(2004) Crit Rev Neurobiol 16, 43-9 I Tapia-Arancibia et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15571756'>(2004) Front Neuroendocrinol 25, 77-107 S Pezet, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12589922'> (2002) Brain Res Brain Res Rev 40, 240-9 JA Coull et al http://www.ncbi.nlm.nih.gov/pubmed/16355225'> (2005) Nature. Dec 15;438(7070):1017-21. C Gomes et al http://www.ncbi.nlm.nih.gov/pubmed/23363775'> (2013) J Neuroinflammation. Jan 30;10:16.
Specificity:
A cross reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by one site ELISA. This antiserum recognises BDNF from rat, mouse and human.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Sheep
Species Reactivity:
Human,Rat
Immunogen:
Recombinant human BDNF
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, Inhibition of biological activity in vitro/in vivo, ELISA. Recommended to be used at an amount of 1-10 µg/mL for immunohistochemistry on Zamboni's fixed, frozen tissue. Not recommended for paraffin embedded tissues. Primary use is for biological activity in vitro and in vivo. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antibody does not react to BDNF in western blot, thus western blot is not a recommended application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Vissio P.G. et al (2008) Brain-derived neurotrophic factor (BDNF)-like immunoreactivity localization in the retina and brain of Cichlasoma dimerus (Teleostei, Perciformes) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18343472'>Tissue Cell. 2008 Aug;40(4):261-70. A Acheson et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7700353'>(1995) Nat. 74: 450-3 Q Yan et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8083736'>(1994) J. Neurosci. 14(9): 5281-91 XF Zhou et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8895863'>(1996) Neurosci. 74: 945-53 XF Zhou, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9454633'>(1998) Exp. Neurol. 149: 237-42 B Mellstrom et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15581398'>(2004) Crit Rev Neurobiol 16, 43-9 I Tapia-Arancibia et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15571756'>(2004) Front Neuroendocrinol 25, 77-107 S Pezet, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12589922'> (2002) Brain Res Brain Res Rev 40, 240-9 JA Coull et al http://www.ncbi.nlm.nih.gov/pubmed/16355225'> (2005) Nature. Dec 15;438(7070):1017-21. C Gomes et al http://www.ncbi.nlm.nih.gov/pubmed/23363775'> (2013) J Neuroinflammation. Jan 30;10:16.
Specificity:
A cross reactivity of less than 1% against mouse NGF, recombinant human NT3 or NT4/5 has been shown by one site ELISA. Known to react with BDNF from rat and human.
Rabbit anti-Glial Fibrillary Acidic Protein (GFAP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Background Info:
Glial Fibrillary Acidic Protein (GFAP) is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas. At least 3 isoforms are produced from alternate splicing. These isoforms differ in the C-terminal region which is encoded by alternative exons.
A synthetic peptide (EMARHLQEYQDLLNVK) corresponding to a region (341-356) from human Glial Fibrillary Acidic Protein. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Applications:
IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgG
Application Details:
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human GFAP has a predicted length of 432 residues and MW of 50 kDa. A concentration of 2.0 µg/mL is recommended to detect GFAP in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Astrocyte; Glial fibrillary acidic protein; GFAP;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Reeves S.A, et al. Proc. Natl. Acad. Sci. U.S.A. 86:5178-5182(1989). Brenner M, et al. Brain Res. Mol. Brain Res. 7:277-286(1990). Isaacs A, et al. Genomics 51:152-154(1998). Ota T, et al. Nat. Genet. 36:40-45(2004). Nielsen A.L, et al. J. Biol. Chem. 277:29983-29991(2002). Singh R, et al. Genomics 82:185-193(2003). Brenner M, et al. Nat. Genet. 27:117-120(2001). Brockmann K, et al. Eur. Neurol. 50:100-105(2003). Stumpf E, et al. Arch. Neurol. 60:1307-1312(2003). Sawaishi Y, et al. Neurology 58:1541-1543(2002). Aoki Y, et al. Neurosci. Lett. 312:71-74(2001).
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse due to sequence homology;
FUNCTION: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. MPO is an important marker for myeloid cells, from the promyelocyte stage and to the mature forms. CATALYTIC ACTIVITY: Donor + H2O2 = oxidized donor + 2 H2O. CATALYTIC ACTIVITY: Cl- + H2O2 = HOCl + 2 H2O. COFACTOR: Binds 1 calcium ion per heterodimer. COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer. SUBUNIT: Tetramer of two light chains and two heavy chains. SUBCELLULAR LOCATION: Lysosome. ALTERNATIVE PRODUCTS: At least 3 named isoforms produced by alternative splicing. DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD). MPD is an autosomal recessive defect that results in disseminated candidiasis. SIMILARITY: Belongs to the peroxidase family. XPO subfamily. Microglia and astrocytes are known to express MPO as well.
A synthetic peptide (KNNIFMSNSYPRDFV) corresponding to a region (714-728) at the C-terminus of human Myeloperoxidase (MPO).
Applications:
IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgG
Application Details:
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human MPO (isoform H17) has a predicted length of 745 residues and MW of 84 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect the protein in formalin/paraffin embedded tissues. Heat mediated antigen retrieval in 10 mM citrate buffer, pH 6.0 for 20 minutes is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
MPO; EC 1.11.1.7; Myeloperoxidase;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Arber D.A. et al. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11447748'> Am J Clin Pathol. 2001; 116:25-33 Kimura S et al. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2840655 '>Proteins 1988;3:113-20. Weil S.C. et al. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2896388'>Science 1988;240:790- Lanza F. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9766845'>J Mol Med 1998;76:676-8 Pinkus G.S. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1724087'>Mod Pathol 1991;4:733-4 Gray E. et al. http://www.ncbi.nlm.nih.gov/pubmed/18042261'>Brain Pathol. 2008 Jan;18(1):86-9 Maki R.A. et al. http://www.ncbi.nlm.nih.gov/pubmed/19059911'>J Biol Chem. 2009 Jan 30;284(5):3158-6
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; predicted to react with mouse
Rabbit anti-Myelin basic protein (MBP) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of approximately 80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP). MBP is an 'intrinsically unstructured' protein with a high proportion (approximately 75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system. A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational. Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic. The message for myelin basic protein is selectively translocated to the ends of the cell processes. Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model. FUNCTION: Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity). SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Myelin membrane; peripheral membrane protein; cytoplasmic side. Cytoplasmic side of myelin. TISSUE SPECIFICITY: Found in both the central and the peripheral nervous system.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Guinea Pig,Human,Rat
Immunogen:
A synthetic peptide (YG SLPQKSQRSQ DENPVV, aa: 68-86) as part of guinea pig MBP protein conjugated to KLH
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1:4000 is recommended. Immunostaining for MBP of abnormal appearing oligodendrocytic process and cell bodies in demyelinating areas. This antibody recognises only areas of myelin degeneration when tested in injured spinal cord and lesioned sciatic nerves. It also stains discrete white matter in the brain of multiple system atrophy. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Myelin Basic Protein
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Schwartz, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12440382'>Prog Brain Res 137, 401-6 (2002) Hauben, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve=PubMed&dopt=Citation&list_uids=11518733'>J Clin Invest 108, 591-9 (Aug, 2001) Yoles, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11356861'>J Neurosci 21, 3740-8 (Jun 1, 2001) Hauben, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10964948'>J Neurosci 20, 6421-30 (Sep 1, 2000) Harauz, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15219899'>Nature 389, 783-4 (1997). Micron 35, 503-42 (2004) Givogri, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10650873'>J Neurosci Res 59, 153-9 (Jan 15, 2000) Kim, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9251242'>Int J Biochem Cell Biol 29, 743-51 (May, 1997) Kalwy, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7522797'>Mol Membr Biol 11, 67-78 (Apr-Jun, 1994) Wajgt, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6198864'>Acta Neurol Scand 68, 337-43 (Nov, 1983) Day, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2418054'>J Neuroimmunol 10, 289-312 (Feb, 1986) 1Mikoshiba, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1709079'>Comp Biochem Physiol C 98, 51-61 (1991) 1Brophy, et al., http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7509522'>Trends Neurosci 16, 515-21 (Dec, 1993) 1Matsuo, A. et al. (1997) Am. J. Pathol. 150(4): 1253-1266
Specificity:
This antiserum recognizes MBP in demyelinated nerve tissues. Immunohistochemical analysis of lesioned rat spinal cord indictaes a high level of specificity for this antiserum. This antiserum reacts with human and rat MBP.
Intercellular adhesion molecule 1 (ICAM1) is a single-pass type 1 membrane glycoprotein that belongs to the ICAM family. ICAM1 is typically expressed on endothelial cells and cells of the immune system. ICAM-1 is also expressed in all types of glial cells including astrocytes and microglia.
A synthetic peptide (YNRQRKIKKYRLQQ) corresponding to a region (503-516) from the C-terminus of human ICAM1.
Applications:
IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgG
Application Details:
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human ICAM1 (precursor) has a predicted length of 532 residues and a MW of 58 kDa. A concentration of 0.5-1 µg/mL is recommended to detect ICAM1 in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Satoh J. et al. Cytokine-induced expression of intercellular adhesion molecule-1 (ICAM-1) in cultured human oligodendrocytes and astrocytes. J Neuropathol Exp Neurol. 1991 May;50(3):215-26. Verbeek M.M. Accumulation of intercellular adhesion molecule-1 in senile plaques in brain tissue of patients with Alzheimer's disease. Am J Pathol. 1994 January; 144(1): 104-11 Heppner F.L. et al. Vitamin E induces ramification and downregulation of adhesion molecules in cultured microglial cells. Glia. 1998 Feb;22(2):180-8. Yang Y. et al. Evaled expression of ICAM-1 and its ligands in the rat spinal cord following lipopolysaccharide intraspinal injection. Neuromolecular Med. 2008;10(4):385-9
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycaemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A peptide (HSDPARRGELSVCDSISEWV) corresponding to a sequence at the N-terminal of human BDNF (amino acids 129-148).
Applications:
WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human BDNF has a predicted length of 247 residues and MW of 28 kDa for BDNF precursor and 13 kDa for mature BDNF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.2-7.6 without preservatives
Host Animal:
Rabbit
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antibody was tested on cultured sensory neurons supported by 100 ng/mL of purified mouse beta NGF. Be advised that 2 µg/mL will neutralize 100 ng/mL of mouse NGF. The higher 10 µg/mL is only recommended if the concentration of NGF being used is higher than 100 ng/mL such as the 200 or 500ng/mL that is occasionally used in some culture systems. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Cassina P. et al (2005) Astrocyte activation by fibroblast growth factor-1 and motor neuron apoptosis: implications for amyotrophic lateral sclerosis. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15773903'> J Neurochem. 2005 Apr;93(1):38-46. Zhou, X. F. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7815824'> (1994) J Neurosci Methods 54, 95-102. Angeletti, P. U. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=4880217'> (1968) Adv Enzymol Relat Areas Mol Biol 31, 51-7 Ebendal, T. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2468788'> (1989) J Neurosci Res 22, 223-240. Hesse K. et al. http://www.ncbi.nlm.nih.gov/pubmed/9291146'> (1997) Neurosci Lett. Aug 8;231(2):83- Miao J et al. http://www.ncbi.nlm.nih.gov/pubmed/23000062'> (2012) Neurosci Res. Dec;74(3-4):269-7
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human BDNF
Applications:
IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, ELISA (1 site), Western Blot, inhibition of biological activity in vitro/in vivo. Recommended to be used at a dilution of 1:1000 for immunohistochemistry, ELISA and Western blot. 1:10 to 1:50 for inhibition of biological activity in vitro. Use neat for in vivo studies at 5-10 uL/g body weight. This antiserum stains cell bodies and some nerve terminals in the dorsal horn of the rat spinal cord, however, does not stain finest nerve terminals. Western Blotting: Antibody does detect BDNF forms in tissue lysates but there are multiple bands present, many of which are uncharacterized. The antibody detects 14 kDa (mature BDNF), 32 kDa (proBDNF) and a 18 kDa BDNF isoform (see blot examples). In cell lysates, only 18 kDa and 32 kDa BDNF are detected. The reason for these differences has not been characterized. Alternative antibodies for Western Blotting are: R-017-500 (IgG-purified form of R-088-100 for tissue homogenate analysis); R-1707-100 (cell lysates and tissue homogenates), R-083-100/R-066-500 (cell lysates, tissue homogenates and human serum); M-1744-50/100 (human serum and tissue homogenates). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A Acheson et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7700353'>(1995) Nat. 74: 450-3 Q Yan et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8083736'>(1994) J. Neurosci. 14(9): 5281-91 XF Zhou et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8895863'>(1996) Neurosci. 74: 945-53 XF Zhou, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9454633'>(1998) Exp. Neurol. 149: 237-42 B Mellstrom et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15581398'>(2004) Crit Rev Neurobiol 16, 43-9 I Tapia-Arancibia et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15571756'>(2004) Front Neuroendocrinol 25, 77-107 S Pezet, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12589922'> (2002) Brain Res Brain Res Rev 40, 240-9 Barde Y. A. et al (1989) EMBO J. 1: 549 Conner J et al. (1997) J. Neurosci. 17: 2295 JA Coull et al http://www.ncbi.nlm.nih.gov/pubmed/16355225'> (2005) Nature. Dec 15;438(7070):1017-21. 1C Gomes et al http://www.ncbi.nlm.nih.gov/pubmed/23363775'> (2013) J Neuroinflammation. Jan 30;10:16.
Specificity:
Less than 0.1% cross-reactivity against NGF, NT3 and NT4/5 by dot blot. Known to react with BDNF from rat, mouse and human. Expected to react with BDNF from other species due to amino acid sequence homology.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide (C-ELLDEDQKVRPNEE) as a part of human BDNF precursor protein (aa: 69-82) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB. A dilution of 1:1000 to 1:5000 is recommended for both applications. ICC: 1:500 to 1:2000, antibody works on 4% formaldehyde fixed cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A Acheson et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7700353'>(1995) Nat. 74: 450-3 Q Yan et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8083736'>(1994) J. Neurosci. 14(9): 5281-91 XF Zhou et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8895863'>(1996) Neurosci. 74: 945-53 XF Zhou, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9454633'>(1998) Exp. Neurol. 149: 237-42 B Mellstrom et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15581398'>(2004) Crit Rev Neurobiol 16, 43-9 I Tapia-Arancibia et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15571756'>(2004) Front Neuroendocrinol 25, 77-107 S Pezet, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12589922'> (2002) Brain Res Brain Res Rev 40, 240-9 Barde Y. A. et al (1989) EMBO J. 1: 549 Conner J et al. (1997) J. Neurosci. 17: 2295 L Ulman, et al http://www.ncbi.nlm.nih.gov/pubmed/18971468'> (2008) J Neurosci. 2008 Oct 29;28(44):11263-8.
Specificity:
Used in western blot, this antiserum detects a 35 kDa band corresponding to the molecular weight of proBDNF. No cross reactivity with other proneurotrophins was detected. This antiserum is known to react with human, mouse and rat proBDNF and also expected to recognise other mammalian proBDNF.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
Mixed
Application Details:
IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A dilution of 1:1000-1:5000 is recommended for IHC, western blot and immunoblot; 1:15000 for ELISA; for inhibition of biological activity: 1:10-50 for in vitro, 5-10 µL/g body weight for in vivo. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Ebendal, T. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2468788'> (1989) J Neurosci Res 22, 223-240. Zhou, X. F. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation_uids=7815824'> (1994) J Neurosci Methods 54, 95-102. Angeletti, P. U. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=4880217'> (1968) Adv Enzymol Relat Areas Mol Biol 31, 51-7 Hesse K. et al. http://www.ncbi.nlm.nih.gov/pubmed/9291146'> (1997) Neurosci Lett. Aug 8;231(2):83- Miao J et al. http://www.ncbi.nlm.nih.gov/pubmed/23000062'> (2012) Neurosci Res. Dec;74(3-4):269-7
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF bot not bovine NGF.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst primates.
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting: This antibody detects multiple BDNF isoforms (14 kDa mature BDNF, 18 kDa isoform, 28 kDa BDNF dimer/truncated BDNF, 32 kDa proBDNF monomer) depending on sample application (human serum, cell lysate, tissue homogenate). Antibody also detects BDNF under non-reducing conditions (McLean NA, 2014). ELISA: Detection only, 1:1000-1:5000 recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A Acheson et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7700353'>(1995) Nat. 74: 450-3 Q Yan et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8083736'>(1994) J. Neurosci. 14(9): 5281-91 XF Zhou et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8895863'>(1996) Neurosci. 74: 945-53 XF Zhou, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9454633'>(1998) Exp. Neurol. 149: 237-42 B Mellstrom et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15581398'>(2004) Crit Rev Neurobiol 16, 43-9 I Tapia-Arancibia et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15571756'>(2004) Front Neuroendocrinol 25, 77-107 S Pezet, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12589922'> (2002) Brain Res Brain Res Rev 40, 240-9 Barde Y. A. et al (1989) EMBO J. 1: 549 Conner J et al. (1997) J. Neurosci. 17: 2295 JA Coull et al http://www.ncbi.nlm.nih.gov/pubmed/16355225'> (2005) Nature. Dec 15;438(7070):1017-21. 1C Gomes et al http://www.ncbi.nlm.nih.gov/pubmed/23363775'> (2013) J Neuroinflammation. Jan 30;10:16.
Specificity:
Less than 0.1% cross reactivity with mouse NGF, recombinant human NT3 and NT4/5 has been recorded by dot blot analysis. This antiserum is known to recognise rat, mouse and human BDNF, and is expected to react with BDNF from other species due to amino acid sequence homology.
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant human GDNF
Applications:
WB
Antibody Isotype:
Mixed
Application Details:
WB and dot blot. Recommended to be used at a dilution of 1:1000 to 1:3000 for Western blot. The predicted molecular weight of the GDNF monomer is 11.6 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer, and Parkinson disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from PBS, pH 7.4, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst mammalian species.
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
Western Blotting: A concentration of 1-10 µg/mL is recommended for this application. In Western Blotting, this antibody detects multiple BDNF isoforms (14 kDa mature BDNF, 18 kDa isoform, 28 kDa BDNF dimer/truncated BDNF, 32 kDa proBDNF monomer) depending on sample application (human serum, cell lysate, tissue homogenate). IHC: Antibody works well in immunohistochemistry with the proper fixation, pretreatments and dilution. Formal fixed, paraffin embedded tissue is not recommend. Recommended fixation is Zamboni fixative or light 4% PFA fixation on fixed, frozen tissue. Recommended dilution is 1-10 µg/mL for immunohistochemistry at 4 degrees centigrade for 2-48 hours. ELISA: 1-10 µg/mL capture/detection. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A Acheson et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7700353'>(1995) Nat. 74: 450-3 Q Yan et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8083736>(1994) J. Neurosci. 14(9): 5281-91 XF Zhou et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8895863>(1996) Neurosci. 74: 945-53 XF Zhou, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9454633>(1998) Exp. Neurol. 149: 237-42 B Mellstrom et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15581398>(2004) Crit Rev Neurobiol 16, 43-9 I Tapia-Arancibia et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15571756>(2004) Front Neuroendocrinol 25, 77-107 S Pezet, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12589922> (2002) Brain Res Brain Res Rev 40, 240-9 Barde Y. A. et al (1989) EMBO J. 1: 549 Conner J et al. (1997) J. Neurosci. 17: 2295 JA Coull et al http://www.ncbi.nlm.nih.gov/pubmed/16355225> (2005) Nature. Dec 15;438(7070):1017-21. C Gomes et al http://www.ncbi.nlm.nih.gov/pubmed/23363775'> (2013) J Neuroinflammation. Jan 30;10:16.
Specificity:
Less than 0.1% cross reactivity with mouse NGF, recombinant human NT3 and NT4/5 has been recorded by dot blot analysis. This antiserum is known to recognise rat, human and human BDNF, and is expected to react with BDNF from other species due to amino acid sequence homology.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human BDNF
Applications:
ELISA,IHC-Frozen,Neutralize,WB
Antibody Isotype:
IgG
Application Details:
IHC, ELISA (1 site), Western Blot, immunoblot, inhibition of biological activity in vitro/in vivo. Recommended to be used at an amount of 1-10 µg/mL for immunohistochemistry, Western blot or immunoblot, 0.1 µg/mL for ELISA and for inhibition of biological activity in vitro 1-10 µg/mL. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum stains cell bodies and some nerve terminals in the dorsal horn of the rat spinal cord, however, does not stain finest nerve terminals. Western blot: 1-10 µg/mL. Tissue homogenate is a recommended sample application for Western Blotting. The antibody detects 14 kDa (mature BDNF), 32 kDa (proBDNF) and a 18 kDa BDNF isoform, however numerous other non-characterized bands may also be present. In cell lysates, only 18 kDa and 32 kDa BDNF are detected. Alternative antibodies for Western Blotting are: R-1707-100 (cell lysates and tissue homogenates), R-083-100/R-066-500 (cell lysates, tissue homogenates and human serum); M-1744-50/100 (human serum and tissue homogenates). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A Acheson et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7700353'>(1995) Nat. 74: 450-3 Q Yan et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8083736'>(1994) J. Neurosci. 14(9): 5281-91 XF Zhou et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8895863'>(1996) Neurosci. 74: 945-53 XF Zhou, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9454633'>(1998) Exp. Neurol. 149: 237-42 B Mellstrom et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15581398'>(2004) Crit Rev Neurobiol 16, 43-9 I Tapia-Arancibia et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15571756'>(2004) Front Neuroendocrinol 25, 77-107 S Pezet, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12589922'> (2002) Brain Res Brain Res Rev 40, 240-9 Barde Y. A. et al (1989) EMBO J. 1: 549 Conner J et al. (1997) J. Neurosci. 17: 2295 JA Coull et al http://www.ncbi.nlm.nih.gov/pubmed/16355225'> (2005) Nature. Dec 15;438(7070):1017-21. C Gomes et al http://www.ncbi.nlm.nih.gov/pubmed/23363775'> (2013) J Neuroinflammation. Jan 30;10:16.
Specificity:
Less than 0.1% cross-reactivity against NGF, recombinant NT3 and NT4 by dot blot. This antiserum is known to react with BDNF from rat, mouse and human. Expected to react with BDNF of other species due to amino acid sequence homology.
Mouse anti-Glial fibrillary acidic protein (GFAP) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Background Info:
GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas. At least 3 isoforms are produced from alternate splicing. These isoforms differ in the C-terminal region which is encoded by alternative exons. This antibody detects all three isoforms.
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-1.0 µg/mL is recommended for WB. Human GFAP has a predicted length of 432 residues and MW of 50 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect GFAP in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Astrocyte; Glial fibrillary acidic protein;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Reeves S.A, et al. Proc. Natl. Acad. Sci. U.S.A. 86:5178-5182(1989). Brenner M, et al. Brain Res. Mol. Brain Res. 7:277-286(1990). Isaacs A, et al. Genomics 51:152-154(1998). Ota T, et al. Nat. Genet. 36:40-45(2004). Nielsen A.L, et al. J. Biol. Chem. 277:29983-29991(2002). Singh R, et al. Genomics 82:185-193(2003). Brenner M, et al. Nat. Genet. 27:117-120(2001). Brockmann K, et al. Eur. Neurol. 50:100-105(2003). Stumpf E, et al. Arch. Neurol. 60:1307-1312(2003). Sawaishi Y, et al. Neurology 58:1541-1543(2002). Aoki Y, et al. Neurosci. Lett. 312:71-74(2001).
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
Mouse anti-Tyrosine Kinase Receptor B (TrkB) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Background Info:
Tyrosine kinase receptor type B (TrkB) is a member of the neurotrophic tyrosine receptor kinase family. It is a membrane-bound receptor and upon neurotrophin binding, it phosphorylates itself as well as MAPK pathways members. TrkB is the receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5, but not nerve growth factor (NGF). It is involved in the development and/or maintenance of the nervous system. It exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. It is a tran-membrane protein. There are 3 named isoforms produced by alternative splicing. Additional isoforms seem to exist. The different forms are differentially expressed in various cell types including neurons and microglia.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant protein of human Tyrosine kinase receptor type B (isoform TrkB-T1) with a GST tag.
Applications:
ELISA,WB
Clone number:
4D3-F10
Antibody Isotype:
IgG2a, kappa
Application Details:
This antibody is recommended for WB, sandwich ELISA and Flow Cytometry. Use 2 ?g/10^6 cells for Flow Cytometry. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Mizoguchi Y et al., J Immunol. 2009 Dec 15;183(12):7778-86. Spencer-Segal JL et al. J Neurosci. 2011 May 4;31(18):6780-90. Nakajima K et al. Glia. 1998 Nov;24(3):272-89.
Specificity:
Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
A synthetic peptide (C-ELLDEDQKVRPNEE) as a part of human BDNF precursor protein (aa: 69-82) conjugated to KLH has been used as the immunogen.
Applications:
IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
IHC, WB. 1-5 µg/mL is recommended for both applications, Flow Cytometry (2?g/10^6 cells). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
A Acheson et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7700353'>(1995) Nat. 74: 450-3 Q Yan et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8083736'>(1994) J. Neurosci. 14(9): 5281-91 XF Zhou et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8895863'>(1996) Neurosci. 74: 945-53 XF Zhou, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9454633'>(1998) Exp. Neurol. 149: 237-42 B Mellstrom et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15581398'>(2004) Crit Rev Neurobiol 16, 43-9 I Tapia-Arancibia et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15571756'>(2004) Front Neuroendocrinol 25, 77-107 S Pezet, et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12589922'> (2002) Brain Res Brain Res Rev 40, 240-9 Barde Y. A. et al (1989) EMBO J. 1: 549 Conner J et al. (1997) J. Neurosci. 17: 2295 L Ulman et al http://www.ncbi.nlm.nih.gov/pubmed/18971468'> (2008) J Neurosci. Oct 29;28(44)
Specificity:
Used in western blot, this antiserum detects a 35 kDa band corresponding to the molecular weight of proBDNF. No cross reactivity with other proneurotrophins was detected. This antibody is known to react with human, mouse and rat proBDNF and also expected to recognise other mammalian proBDNF.
The protein named TrkB (also named Neurotrophic tyrosine kinase receptor type 2 (NTRK2), GP145-TrkB or Tropomyosin-related kinase B is a receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems and is important in the regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. TrkB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. TrkB is the primary receptor for BDNF (brain-derived neurotrophic factor. TrkB also binds NT4 and NT3 but less efficiently. Upon ligand-binding, the receptor undergoes homodimerization, autophosphorylation and activation. TrkB activation recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that each regulate distinct overlapping signaling cascades within cells. Through SHC1, FRS2, SH2B1, SH2B2, these activate the GRB2-Ras-MAPK cascade that regulates, for instance, neuronal differentiation including neurite outgrowth. These same effectors also control the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. TrkB, via activation of PLCG1 and the downstream protein kinase C-regulated pathways, also controls synaptic plasticity, and thus plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. One such consequence is that PLCG1 activation via TrkB is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. (Reference: www.uniprot.org)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, PBS, pH 7.2-7.6, 0.1% trehalose, without preservatives.
Western Blotting (0.5 - 2 µg/mL). Cell lysates or membrane preparations prepared from isolated brain or spinal cord tissues are recommended. This antibody works on skim milk blocked membranes, however, best results are obtained with a equal mixture of 2.5% skim milk / 2.5% highly purified BSA as blocking reagent and antibody diluent. Immunohistochemistry (1 - 5 µg/mL). Antibody has been shown to work on PFA fixed, frozen sections. TBS is preferred for buffer preparation. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Detects full-length TrkB phosphorylated at amino acid S479 in humans (S478 in mouse and rat).
Positive Control:
Retinoic acid- and BDNF-treated NSC34 cells
References:
Lai K-O. et al. (2012), Nature Neuroscience 15, 1506-1515, doi:10.1038/nn.3237. Zhao L. et al. (2009), Journal of Cell Science 122, 3123-3136, doi: 10.1242/jcs.047712.
Specificity:
Human TrkB (pS478). Antibody has been shown to be specific for TrkB phosphorylated on serine 478 by phospho-peptide absorption dot blots, and on cell lysates from cell lines induced with retinoic acid and BDNF. Antibody detects a clear band in retinoic acid (RA) and BDNF-treated NSC34 cell lysates at ~120 kDa only, demonstrating that the phosphorylated TrkB receptor is being detected. Additional non-specific bands at lower molecular weight may be observed in lysates and homogenates with the antibody and these bands have not been characterized.
Product Validation Info:
Antibody is specific for TrkB serine phosphorylation at amino acid 478/479 (rodents/human). Lambda-phosphatase treatment obliterates positive staining.
The protein named TrkB (also named Neurotrophic tyrosine kinase receptor type 2 (NTRK2), GP145-TrkB or Tropomyosin-related kinase B is a receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems and is important in the regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. TrkB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. TrkB is the primary receptor for BDNF (brain-derived neurotrophic factor. TrkB also binds NT4 and NT3 but less efficiently. Upon ligand-binding, the receptor undergoes homodimerization, autophosphorylation and activation. TrkB activation recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that each regulate distinct overlapping signaling cascades within cells. Through SHC1, FRS2, SH2B1, SH2B2, these activate the GRB2-Ras-MAPK cascade that regulates, for instance, neuronal differentiation including neurite outgrowth. These same effectors also control the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. TrkB, via activation of PLCG1 and the downstream protein kinase C-regulated pathways, also controls synaptic plasticity, and thus plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. One such consequence is that PLCG1 activation via TrkB is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. (Reference: www.uniprot.org)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized, PBS, pH 7.2-7.6, 0.1% trehalose, without preservatives.
Western Blotting (0.5 2 µg/mL). High skim milk concentration (5%) causes suppression of pY817 signal, suggesting that 5% skim milk should be avoided as blocking buffer and antibody diluent. Strongest signal is obtained in 5% BSA blocking buffer, however, many non-specific bands are present. An equal mixture of skim milk and BSA (2.5% each) appears to provide the best compromise between signal and noise. However, optimization of blocking condition is recommended for each particular sample, with excess of BSA over skim milk likely to be beneficial for best results. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Detects full-length TrkB phosphorylated at amino acid Y817 in humans (Y816 in mouse and rat).
Positive Control:
Retinoic acid- and BDNF-treated NSC34 cells
Specificity:
Human TrkB (pY817). Antibody has been shown to be specific for TrkB phosphorylated on tyrosine 817 by phospho-peptide absorption dot blots, and on cell lysates from cell lines induced with retinoic acid and BDNF. Antibody detects a clear band in retinoic acid (RA) and BDNF-treated NSC34 cell lysates at ~120 kDa only, demonstrating that the phosphorylated TrkB receptor is being detected. While not fully tested, this antibody may detect phosphorylated TrkA (pY791/794, human/rodent) and TrkC (pY834/820/859, human/mouse/rat) due to high degree of amino acid homology surrounding the phosphorylation site.
Product Validation Info:
Antibody detects a clear band in retinoic acid (RA) and BDNF-treated NSC34 cell lysates at ~120 kDa only
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.05% sodium azide.
Host Animal:
Rabbit
Species Reactivity:
Human,Other Mammals (Predicted)
Immunogen:
Antibody was raised against a GST-tagged rhBDNF fusion protein and expressed in and purified from E. coli.
Applications:
ELISA
Antibody Isotype:
IgG
Application Details:
Western Blotting (denaturing and reducing): 0.2 to 1 µg/mL. Antibody detects 14 kDa mature BDNF monomer and 32 kDa proBDNF monomer in cell lysate and tissue homonenate. Antibody has only been tested on cell lysate and tissue homogenate of human origin. Acid-treated samples may give cleaner blots, and enhance signals for BDNF. R-1707-100 is not recommended for human serum samples. For human serum analysis, we recommend mouse monoclonal antibody to rhBDNF (M-1744-50/100), or rabbit polyclonal antibody to BDNF peptide 1-10 (R-083-100, whole serum; R-066-500, IgG). Flow Cytometry: ~2 µg per 10^6 cells, methanol fixation. Note: R-1707-100 cannot be used to distinguish the flow cytometry signal originating from mature BDNF versus proBDNF. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Brain-derived neurotrophic factor; Abrineurin
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Human, rat and mouse BDNF. Expected to detect BDNF from other species due to sequence homology. No cross-reactivity with other neurotrophins.
CD40L is the ligand for CD40, a member of the tumour necrosis factor (TNF) receptor superfamily. CD40L is expressed mainly on activated CD4+ T-lymphocytes as a single-pass type II membrane protein. Proteolytic processing of the membrane form results in a soluble secreted form of CD40L. Microglia and astrocytes interact via CD40 receptor and CD40 ligand.
A synthetic peptide (HRRLDKIEDERNLHEDFVF) corresponding to amino acid sequence 47-65 from the N-terminus of human CD40L.
Applications:
IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgG
Application Details:
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 µg/mL is recommended for WB. Human CD40L has a predicted length of 261 residues and MW of 29 kDa. The observed size is 32 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect CD40L in formalin fixed and paraffin embedded tissues. Heat mediated antigen retrieval is required. Boiling the paraffin sections in 10mM citrate buffer, pH 6.0 for 20 minutes is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Ke Z.J. et al. CD40-CD40L interactions promote neuronal death in a model of neurodegeneration due to mild impairment of oxidative metabolism. Neurochem Int. 2005 Aug;47(3):204-1
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human;
Rabbit anti-Myelin basic protein (MBP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded.
Background Info:
Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of approximately 80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP). MBP is an 'intrinsically unstructured' protein with a high proportion (approximately 75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system. A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational. Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic. The message for myelin basic protein is selectively translocated to the ends of the cell processes. Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model. FUNCTION: Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity). SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Myelin membrane; peripheral membrane protein; cytoplasmic side. Cytoplasmic side of myelin. TISSUE SPECIFICITY: Found in both the central and the peripheral nervous system.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3 added as preservative
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse (Predicted),Rat
Immunogen:
A synthetic peptide corresponding to a region (289-304 aa) from the C-terminus of human Myelin Basic Protein (MBP). This sequence also corresponds to a region on rat MBP (180-195 aa).
Applications:
IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgG
Application Details:
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human MBP (isoform #1, Golli-MBP1) has a predicted length of 304 residues and MW of 33 kDa. A concentration of 1.0 µg/mL is recommended to detect MBP in paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Schwartz, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12440382">Prog Brain Res 137, 401-6 (2002) Hauben, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/11518733">J Clin Invest 108, 591-9 (Aug, 2001) Yoles, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11356861">J Neurosci 21, 3740-8 (Jun 1, 2001) Hauben, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10964948">J Neurosci 20, 6421-30 (Sep 1, 2000) Harauz, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15219899">Nature 389, 783-4 (1997). Micron 35, 503-42 (2004) Givogri, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10650873">J Neurosci Res 59, 153-9 (Jan 15, 2000) Kim, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9251242">Int J Biochem Cell Biol 29, 743-51 (May, 1997) Kalwy, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7522797">Mol Membr Biol 11, 67-78 (Apr-Jun, 1994) Wajgt, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6198864">Acta Neurol Scand 68, 337-43 (Nov, 1983) Day, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2418054">J Neuroimmunol 10, 289-312 (Feb, 1986) 1Mikoshiba, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1709079">Comp Biochem Physiol C 98, 51-61 (1991) 1Brophy, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7509522">Trends Neurosci 16, 515-21 (Dec, 1993) 1Matsuo, A. et al. <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1858181/">(1997) Am. J. Pathol. 150(4): 1253-1266
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat; expected to react with mouse due to sequence homology
Rabbit anti-Tyrosine Kinase Receptor B (TrkB) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen, ELISA.
Background Info:
TrkB is a member of the neurotrophic tyrosine receptor kinase family. It is a membrane-bound receptor and upon neurotrophin binding, it phosphorylates itself as well as MAPK pathways members. TrkB is the receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). It is Involved in the development and/or maintenance of the nervous system. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 4 named isoforms produced by alternative splicing. Additional isoforms seem to exist. TISSUE SPECIFICITY: The different forms are differentially expressed in various cell types. SIMILARITY: Belongs to the Tyr protein kinase family. Insulin receptor subfamily. SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SIMILARITY: Contains 2 LRR (leucine-rich) repeats. SIMILARITY: Contains 1 protein kinase domain. Mutations in the TrkB gene have been associated with obesity and mood disorders.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Mouse,Rat
Immunogen:
Extracellular domain of glycosylated mouse TrkB protein produced in CHO cells was used as the immunogen. As shown for similar antisera, it is anticipated that this antibody will block the TrkB receptor binding of corresponding neurotrophin ligand.
Applications:
ELISA,IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IF (1:1000-1:3000), 1-site ELISA (1:10,000 dilution). A dilution of 1:1000 to 1:3000 o/n is recommended for IF. 4% PFA frozen sections tested. Not yet tested on paraffin embedded tissues. Use triton X-100 permeabilizaiton with frozen sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Desmet CJ, Peeper DS (2006) Cell Mol Life Sci. 63(7-8) pp. 755-9 Mizoguchi Y et al., J Immunol. 2009 Dec 15;183(12):7778-8 Spencer-Segal JL et al. J Neurosci. 2011 May 4;31(18):6780-90. Nakajima K et al. Glia. 1998 Nov;24(3):272-8
Specificity:
Specificity was demonstrated by immunohistochemistry. This antibody was used to stain cryostat sections of the rat peripheral sensory ganglia. Reacts with rat and mouse TrkB. Other species have not yet been tested.
Rabbit anti-Glial Fibrillary Acidic Protein (GFAP) Polyclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
Background Info:
GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Bovine,Horse,Human,Mouse,Pig,Rat
Immunogen:
Recombinant full length human GFAP isotype 1 expressed in and purified from E. coli.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:5,000 is recommended for WB, and 1:1,000 - 1:5,000 for ICC and IHC. A dilution of 1:1,000 using fluorescent secondary antibodies or 1:5,000 using peroxidase or other enzyme-linked methods is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Astrocyte; Glial fibrillary acidic protein; GFAP;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Reeves S.A, et al. Proc. Natl. Acad. Sci. U.S.A. 86:5178-5182(1989). Brenner M, et al. Brain Res. Mol. Brain Res. 7:277-286(1990). Isaacs A, et al. Genomics 51:152-154(1998). Ota T, et al. Nat. Genet. 36:40-45(2004). Nielsen A.L, et al. J. Biol. Chem. 277:29983-29991(2002). Singh R, et al. Genomics 82:185-193(2003). Brenner M, et al. Nat. Genet. 27:117-120(2001). Brockmann K, et al. Eur. Neurol. 50:100-105(2003). Stumpf E, et al. Arch. Neurol. 60:1307-1312(2003). Sawaishi Y, et al. Neurology 58:1541-1543(2002). Aoki Y, et al. Neurosci. Lett. 312:71-74(2001).
Rabbit anti-Caspase-8 (CASP-8) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Caspase-8 is involved in the programmed cell death induced by Fas and various apoptotic stimuli. Many alternatively spliced transcript variants encoding different isoforms have been described. (Ref: Entrez Gene). Recent observations in microglia have implicated caspase 8 as a modulator of microglial activation in absence of cell death.
A synthetic peptide (DKVYQMKSKPRGYCLIINNH) corresponding to a region (223-242 aa) from human Caspase-8.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB). A concentration of 1.0 µg/mL is recommended for WB. Human Caspase-8 (Isoform 1 precursor) has a predicted length of 479 amino acids and MW of 55 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Rabbit anti-Coronin- 1A Polyclonal Antibody (Unconjugated), suitable for WB, ICC.
Background Info:
Coronins belong to the WD40 or WD family of proteins. Coronins appear to be particularly involved in binding to actin, actin associated proteins, tubulin and phospholipase C and have been implicated in the mechanisms of chemotaxis and phagocytosis. In mammals there are at least five major coronin proteins, named coronins 1 to 5 in one nomenclature. Another nomenclature divides these five proteins in coronins 1a and 1b, 2a, 2b and 2c (see the Human Genone Organization Gene Nomenclature Committee link for this family). The mammalian coronin family members are abundant components of eukaryotic cells and each type has a restricted cell type specific expression pattern. Coronin 1A is found exclusively in hematopoetic lineage cells such as lymphocytes, macrophages and neutrophils. This antibody is therefore an excellent marker of cells of this lineage and can also be used to study the leading edges particularly of neutrophils. Since the only hematopoetic cells found within the central nervous system are microglia, this antibody is also an excellent marker of this important cell type. Microglia are numerically fairly minor components of the nervous system, but microglial activation is seen in response to a wide variety of damage and disease states, including ALS, Alzheimer's disease and responses to brain tumors. Since coronin 1a is a constitutive component of microglia, the coronin 1a antibody can be used to study both quiescent and activated microglia.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
C-terminal peptide of human coronin 1a coupled to KLH
Applications:
ICC,WB
Antibody Isotype:
Mixed
Application Details:
Western Blotting (WB) and Immunocytochemistry (ICC). A dilution of 1:2,500-5,000 is recommended for WB. Human Coronin 1A has a predicted length of 461 residues and a MW of 51 kDa. A concentration of 1:500-1:1,000 is recommended for ICC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Coronin-1A; Coronin-like protein A; Clipin-A; Coronin-like protein p57; Tryptophan aspartate-containing coat protein; TACO; CORO1A; CORO1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by WB and ICC against the antigen. Human, Rat, Mouse and Feline. Predicted to react with other mammalian tissue.
Rabbit anti-Tyrosine Kinase Receptor B (TrkB) Polyclonal Antibody (Unconjugated), suitable for IHC-Frozen.
Background Info:
FUNCTION: Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures (By similarity). Binds APS. Interacts with SQSTM1. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. Additional isoforms seem to exist. TISSUE SPECIFICITY: The different forms are differentially expressed in various cell types. Isoform T2 is primarily expressed in neurons. PTM: Ligand-mediated auto-phosphorylation. SIMILARITY: Belongs to the Tyr protein kinase family. Insulin receptor subfamily. SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SIMILARITY: Contains 2 LRR (leucine-rich) repeats. SIMILARITY: Contains 1 protein kinase domain.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide (AFPRLEPNSIDPEN(C)) as part of rat TrkB protein (aa: 54-67) conjugated to KLH
Applications:
IHC-Frozen
Antibody Isotype:
Mixed
Application Details:
IHC. A dilution of 1:1000 to 1:3000 is recommended for this application. For WB, a 1:500 to 1:1000 dilution is recommended. The sequence of the immunogen and the conjugate are identical to the one used by Yan et al (see the Refs) and the antiserum appears to have similar characteristics. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Yan Q et al. J Comp Neurol 378:135-57 (1997). Stoilov, P., et al., Biochem. Biophys. Res. Commun. 290(3):1054-1065 (2002). Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Nakagawara, A., et al., Genomics 25(2):538-546 (1995). Haniu, M., et al., Arch. Biochem. Biophys. 322(1):256-264 (1995). Allen, S.J., et al., Neuroscience 60(3):825-834 (1994). Mizoguchi Y et al., J Immunol. 2009 Dec 15;183(12):7778-8 Spencer-Segal JL et al. J Neurosci. 2011 May 4;31(18):6780-90. Nakajima K et al. Glia. 1998 Nov;24(3):272-8
Specificity:
Sequence was chosen to avoid cross reactivity with TrkA and TrkC. This antiserum recognises human, rat and mouse TrkB. Cross reactivity with other species has not yet been tested.
Mouse anti-Tyrosine Kinase Receptor C (TrkC) Monoclonal Antibody (Unconjugated), suitable for FC, ICC.
Background Info:
Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation (Ref: uniprot.org).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS, pH 7.2-7.6 with 0.1% trehalose and contains no preservatives.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic peptide immunogen, KEPFPESTDNFI, cooresponding to amino acids 397-408 of human TrkC ECD.
Applications:
FC,ICC
Clone number:
BS337
Antibody Isotype:
IgG2b, kappa
Application Details:
Flow Cytometry (5-10 µg/mL): Tested on human and rodent cell lines. Cell staining can be performed under native conditions on ice, or on fixed cells with up to 4% formaldehyde. Immunocytochemistry (1-2 µg/mL): Tested on fixed (4% formaldehyde) human cells. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Mouse anti-Tyrosine Kinase Receptor B (TrkB) Monoclonal Antibody (Unconjugated), suitable for FC.
Background Info:
The protein named TrkB (also named Neurotrophic tyrosine kinase receptor type 2 (NTRK2), GP145-TrkB or Tropomyosin-related kinase B is a receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems and is important in the regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. TrkB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. TrkB is the primary receptor for BDNF (brain-derived neurotrophic factor. TrkB also binds NT4 and NT3 but less efficiently. Upon ligand-binding, the receptor undergoes homodimerization, autophosphorylation and activation. TrkB activation recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that each regulate distinct overlapping signaling cascades within cells. Through SHC1, FRS2, SH2B1, SH2B2, these activate the GRB2-Ras-MAPK cascade that regulates, for instance, neuronal differentiation including neurite outgrowth. These same effectors also control the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. TrkB, via activation of PLCG1 and the downstream protein kinase C-regulated pathways, also controls synaptic plasticity, and thus plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. One such consequence is that PLCG1 activation via TrkB is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. (Reference: www.uniprot.org)
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS, pH 7.2-7.6 with 0.1% trehalose.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic peptide immunogen, ESSKNIPLANLQ. Extracellular domain of TrkB (amino acids 179-190 of human TrkB).
Applications:
FC
Clone number:
BS379
Antibody Isotype:
IgG2b, kappa
Application Details:
Flow Cytometry (5-10 µg/mL): Tested on human and rodent cell lines. Cell staining can be performed under native conditions on ice, or on fixed cells with up to 4% formaldehyde. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Mouse,Pig,Rat
Immunogen:
GFAP isolated biochemically from pig spinal cord was used as the immunogen.
Applications:
IHC-Frozen,WB
Clone number:
2A5
Antibody Isotype:
IgG1
Application Details:
Western Blot (1:1,000-1:2,000): tested on rat, mouse brain and spinal cord, human recombinant protein, pig brain. Immunohistochemistry (1:500-1:1,000): tested on rat cerebellum section. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Glial fibrillary acidic protein; GFAP
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
This antibody is specific for GFAP as demonstrated by western blotting and immunohistochemistry.
May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS buffer pH 7.2-7.6 with 3% trehalose, without preservatives.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Full-length recombinant human protein
Applications:
ICC,WB
Clone number:
1H10
Antibody Isotype:
IgG1
Application Details:
Western blotting (1:5,000-1:20,000) and Immunocytochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Protein S100 beta belongs to the S100 family of proteins containing 2 EF-hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Among brain cells, S100 beta is often used as a marker for astrocytes. The 2 subunits of S100 protein are the products of separate genes. The beta subunit is present in all S100 positive cells and tumours whereas the alpha subunit is detectable only in specific tissues such as heart, skeletal muscle and brain. The S100 protein is a homodimer composed of two alpha subunits (S100a) or two beta subunits (S100b) or as a heterodimer with one alpha and beta subunit (S100ab or S100ao). This antibody reacts specifically with the beta subunit.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from mouse ascites fluid containing 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative.
Immunohistochemistry (IHC). A concentration of 1.0-2.0 µg/mL is recommended to detect S100 beta subunit in paraffin embedded tissues. Antigen retrieval by heat is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
S100B; Calcium binding protein B1;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
The specificity of this antibody has been confirmed by IHC against the antigen. Human, rabbit and rat. May cross-react with horse and monkey S100-beta based on amino acid sequence similarities.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human mature BDNF expressed in E.coli
Applications:
ELISA,WB
Clone number:
4C8
Antibody Isotype:
IgG
Application Details:
ELISA: 50-100 ng/mL Western Blotting (denaturing and reducing): 0.2 to 2 µg/mL. M-1744-100 antibody detects 14 kDa mature BDNF monomer in human serum human and acid treated human and rodent whole tissue homogenates. In simple Tris-homogenate human brain lysates, M-1744-100 detects mature BDNF at 14kDa as well as an uncharacterized band at ~50kDa. No proBDNF band is detected in our samples, although M-1744-100 will detect proBDNF in purified form on blots. Additionally, M-1744-100 detects an additional band 18 kDa BDNF isoform human serum. For cell lysates, some caution is advised as in our hands M-1744-100 fails to detect mature BDNF on SH-SY5Y or C6 cell lysates but does detect mature BDNF in neuronal cell culture lysates. The reason for these differences has not been characterized. For detection of mature BDNF in cell lysates such as SH-SY5Y we recommend affinity-purified rabbit polyclonal antibody to rhBDNF (R-1707-100), or rabbit polyclonal antibody to BDNF peptide 1-10 (R-083-100, whole serum; R-066-500, IgG). Immunofluorescence: 1 to 4 µg/mL, 4% formaldehyde fixation and permeabilization. Staining can be weak until optimized. M-1744-100 is not recommended for Flow Cytometry at this time. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human mature BDNF expressed in E.coli
Applications:
ELISA,WB
Clone number:
4C8
Antibody Isotype:
IgG
Application Details:
ELISA: 50-100 ng/mL Western Blotting (denaturing and reducing): 0.2 to 2 µg/mL. M-1744-100 antibody detects 14 kDa mature BDNF monomer in human serum human and acid treated human and rodent whole tissue homogenates. In simple Tris-homogenate human brain lysates, M-1744-100 detects mature BDNF at 14kDa as well as an uncharacterized band at ~50kDa. No proBDNF band is detected in our samples, although M-1744-100 will detect proBDNF in purified form on blots. Additionally, M-1744-100 detects an additional band 18 kDa BDNF isoform human serum. For cell lysates, some caution is advised as in our hands M-1744-100 fails to detect mature BDNF on SH-SY5Y or C6 cell lysates but does detect mature BDNF in neuronal cell culture lysates. The reason for these differences has not been characterized. For detection of mature BDNF in cell lysates such as SH-SY5Y we recommend affinity-purified rabbit polyclonal antibody to rhBDNF (R-1707-100), or rabbit polyclonal antibody to BDNF peptide 1-10 (R-083-100, whole serum; R-066-500, IgG). Immunofluorescence: 1 to 4 µg/mL, 4% formaldehyde fixation and permeabilization. Staining can be weak until optimized. M-1744-100 is not recommended for Flow Cytometry at this time. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human mature BDNF expressed in E.coli
Applications:
ELISA,ICC,WB
Clone number:
3C11
Antibody Isotype:
IgG
Application Details:
ELISA (50-100 ng/mL), WB (0.2 to 2 µg/mL), IF (2 to 20 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
BDNF belongs to the neurotrophin family and promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.1% sodium azide.
Host Animal:
Mouse
Species Reactivity:
Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant human mature BDNF expressed in E.coli
Applications:
ELISA,ICC,WB
Clone number:
3C11
Antibody Isotype:
IgG
Application Details:
ELISA (50-100 ng/mL), WB (0.2 to 2 µg/mL), IF (2 to 20 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Goat
Species Reactivity:
Avian,Human,Mouse,Rat
Immunogen:
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
Mixed
Application Details:
IHC, WB and direct ELISA. A dilution range of 1:1000 - 1:5000 is recommended for IHC and WB. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Beta-nerve growth factor; Beta-NGF; NGF; NGFB;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Ebendal, T. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2468788'> (1989) J Neurosci Res 22, 223-240. Zhou, X. F. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation_uids=7815824'> (1994) J Neurosci Methods 54, 95-102. Angeletti, P. U. et al http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=4880217'> (1968) Adv Enzymol Relat Areas Mol Biol 31, 51-75. Hesse K. et al. http://www.ncbi.nlm.nih.gov/pubmed/9291146'> (1997) Neurosci Lett. Aug 8;231(2):83-6. 5. Miao J et al. http://www.ncbi.nlm.nih.gov/pubmed/23000062'> (2012) Neurosci Res. Dec;74(3-4):269-76.
Specificity:
A cross reactivity of less than 1% to recombinant human BDNF, NT3, NT4/5 by ELISA has been shown. This antiserum is known to cross react with mouse, rat, human and avian NGF but not bovine NGF. Other species have not yet been tested.
May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from a solution containing PBS buffer pH 7.2-7.6, without preservatives.
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Full-length recombinant human protein
Applications:
ICC,WB
Antibody Isotype:
IgY
Application Details:
Western blotting (1:5,000-1:10,000), Immunocytochemistry (1:5,000-1:10,000) and Immunohistochemistry (1:5,000-1:10,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by noncovalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two mouse beta NGF peptides (74-85 and 113-124 aa).
Applications:
ELISA,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
ELISA, WB and IHC. WB suggested dilution of 1:500-1:2,000. IHC suggested dilution of 1:50-1:1,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Beta-nerve growth factor; Beta-NGF; NGF; NGFB;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Hesse K et al., Neurosci Lett. 1997 Aug 8;231(2):83-6. Miao J et al. Neurosci Res. 2012 Dec;74(3-4):269-76.
Specificity:
Human beta NGF, cross reactivity to other species NGF is expected
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Mixture of two human GDNF peptides (101-118 and 199-211 aa). Both peptides are highly conserved in human and mouse.
Applications:
ELISA,ICC,IHC-Frozen,WB
Antibody Isotype:
IgY
Application Details:
ELISA, WB and IHC. WB suggested dilution of 1:500-1:2,000. IHC suggested dilution of 1:50-1:500. Biosensis recommends that the optimal working dilution should be determined by the end user.
Lin et al <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8493557">(1993) Science. 260(5111):1130-2
Integrin alpha-6/beta-1 (ITGA6:ITGB1) is a receptor for laminin on platelets (By similarity). Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-6/beta-4 (ITGA6:ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464). Source: uniprot.org. In new work published by L.Barbar [PPR83158] the authors demonstrate that CD49f is an effective marker effective marker for sorting functional human astrocytes from mixed cultures.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
E.coli-derived human Integrin alpha 6/ITGA6 (CD49f) recombinant protein (amino acids Q189-K917).
Applications:
ELISA,WB
Antibody Isotype:
IgG
Application Details:
Western blotting (0.1-0.5 µg/mL) and direct ELISA (0.1-0.5 µg/mL). Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Integrin alpha-6; CD49 antigen-like family member F; VLA-6; CD_antigen: CD49f
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Specificity:
Rabbit IgG polyclonal antibody for Integrin alpha 6/ITGA6 detection. Tested in WB, and direct ELISA against Human, Mouse, and Rat extracts.
Rabbit anti-Tyrosine Kinase Receptor C (TrkC) Polyclonal Antibody (Unconjugated), suitable for WB.
Background Info:
Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation (Ref: uniprot.org).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from a solution containing 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Synthetic peptide immunogen, QLWQEQGEAKLNSQN from the ECD of human TrkC, amino acids 172-186.
Applications:
WB
Antibody Isotype:
IgG
Application Details:
Western Blotting (0.1 - 0.5 µg/mL): Tested on RIPA lysates from human and rodent tissue and cell lysates. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human TrkC, and Mouse and rat TrkC. Species cross-reactivity determined by Western Blotting. No Cross reactivity by WB seen with TrkA or TrkB receptors.
Rabbit anti-Tyrosine Kinase Receptor B (TrkB) Polyclonal Antibody (Unconjugated), suitable for WB, FC.
Background Info:
The protein named TrkB (also named Neurotrophic tyrosine kinase receptor type 2 (NTRK2), GP145-TrkB or Tropomyosin-related kinase B is a receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems and is important in the regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. TrkB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. TrkB is the primary receptor for BDNF (brain-derived neurotrophic factor. TrkB also binds NT4 and NT3 but less efficiently. Upon ligand-binding, the receptor undergoes homodimerization, autophosphorylation and activation. TrkB activation recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that each regulate distinct overlapping signaling cascades within cells. Through SHC1, FRS2, SH2B1, SH2B2, these activate the GRB2-Ras-MAPK cascade that regulates, for instance, neuronal differentiation including neurite outgrowth. These same effectors also control the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. TrkB, via activation of PLCG1 and the downstream protein kinase C-regulated pathways, also controls synaptic plasticity, and thus plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. One such consequence is that PLCG1 activation via TrkB is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. (Reference: www.uniprot.org)
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from a solution containing 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3.
Western Blotting (0.1 - 0.5 µg/mL): Tested on RIPA lysates from human and rodent brain tissue. Detects TrkB full-length (~140 kDa) and truncated TrkB (~100 kDa) in tissue homogenates. In cell lysates, only a ~50 kDa uncharacterized TrkB isoform is detected. Flow Cytometry (5-20 µg/mL): Tested on human cell lines. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Trk-B,TrkB, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
Positive Control:
Rat or mouse cortex (WB). SHSY5Y cell line (FC).
Specificity:
TrkB; detects TrkB in both human, mouse and rat samples by western blot and has positive staining by Flow cytometry against human TrkB positive cell lines.
Rabbit anti-Caspase-3 (CASP-3) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded, FC.
Background Info:
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
E.coli-derived human Caspase 3 recombinant protein (Position: T67-D175) was used as the immunogen. Human Caspase 3 shares 86% and 90% amino acid (aa) sequences identity with mouse and rat Caspase 3, respectively.
Applications:
FC,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgG
Application Details:
Western Blot : (0.1-0.5 µg/mL) tested in human hepatocarcinoma cell line SMMC, rat liver tissue, and rat thymus tissue. Immunohistochemistry : formalin fixed, paraffin embedded sections (0.5-1.0 µg/mL): with HIER; tested in human lung cancer tissue, rat intestine tissue, mouse intestine tissue,. Flow Cytometry (1-3 ?g/10e6 cells): tested in human cell line K562. Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized whole serum without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
C-terminal peptide of human IBA1 protein coupled to KLH.
Applications:
ICC,IHC-Frozen,WB
Antibody Isotype:
IgG
Application Details:
Western blotting (1:1,000-1:5,000) and Immunohistochemistry (1:2,000-1:5,000); Immunocytochemistry (1:1,000-1:5,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin. Ref: uniprot.org
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized from a solution containing PBS buffer pH 7.2-7.6 with 3% trehalose, without preservatives.
Host Animal:
Rabbit
Species Reactivity:
Human,Mouse,Rat
Immunogen:
Full-length recombinant human protein
Applications:
ICC,WB
Antibody Isotype:
IgG
Application Details:
Western blotting (1:1,000-1:5,000) and Immunocytochemistry (1:1,000-1:2,000). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimer's, and Parkinson's disease. Microglia release BDNF that may contribute to neuroinflammation and neuropathic pain. FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. POst translation modification: Converted into mature BDNF by plasmin (PLG). SIMILARITY: Belongs to the NGF-beta family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Host Animal:
Chicken
Species Reactivity:
Human,Mouse
Immunogen:
Mixture of two human BDNF peptides (73-87 and 194-208 aa). Both peptides are highly conserved in human and mouse.
Applications:
ELISA
Antibody Isotype:
IgY
Application Details:
ELISA. Suggested dilution at 1:500 to 1:2,000. Biosensis recommends that the optimal working dilution should be determined by the end user.
JA Coull et al http://www.ncbi.nlm.nih.gov/pubmed/16355225'> (2005) Nature. Dec 15;438(7070):1017-21. C Gomes et al http://www.ncbi.nlm.nih.gov/pubmed/23363775'> (2013) J Neuroinflammation. Jan 30;10:16.
Chicken anti-Myelin basic protein (MBP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ICC.
Background Info:
Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of approximately 80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP). MBP is an 'intrinsically unstructured' protein with a high proportion (approximately 75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system. A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational. Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic. The message for myelin basic protein is selectively translocated to the ends of the cell processes. Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model. FUNCTION: Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity). SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Myelin membrane; peripheral membrane protein; cytoplasmic side. Cytoplasmic side of myelin. TISSUE SPECIFICITY: Found in both the central and the peripheral nervous system.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with 5% trehalose
Host Animal:
Chicken
Species Reactivity:
Bovine,Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Three peptide sequences conserved in higher verterbrate MBP protein.
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). The recommended dilution for WB is 1:5,000-10,0000 and 1:500-1,000 for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
The specificity of this antibody has been confirmed by WB. This antibody stains a prominent band at approx. 20 kDa. A suitable control tissue is rat spinal cord or peripheral nerve homogenate. The major isoforms of MBP run as a closely spaced double of 22 kDa and 18 kDa. Hu, Rat, Ms, Bov. Predicted to react with other mammalian tissues due to sequence homology.
Chicken anti-Glial fibrillary acidic protein (GFAP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Paraffin-embedded, IHC-Frozen, ICC.
Background Info:
Glial fibrillary acidic protein (GFAP) is approx. 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with 5% trehalose. The IgY preparation is at a concentration of ~25 mg/mL total protein.
Host Animal:
Chicken
Species Reactivity:
Cat,Human,Mouse,Other Mammals (Predicted),Rat
Immunogen:
Recombinant GFAP (expressed in E.coli) and native bovine GFAP
Applications:
ICC,IHC-Frozen,IHC-Paraffin-embedded,WB
Antibody Isotype:
IgY
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). WB: A dilution of 1:5,000 is recommended. ICC: A dilution of 1:1,000-1:5,000 using fluorescent secondary antibodies or peroxidase or other enzyme-linked methods is recommended on 4% PFA fixed cells in culture, with 3hr-o/n incubation of primary antibody. IHC: 4% PFA frozen tissues, permeabilized. IHC (paraffin-embedded): capable, HEIR treatment typically necessary. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Astrocyte; Glial fibrillary acidic protein; GFAP;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Brenner M. et al (2001) Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease. <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/11138011?"> Nat Genet. 2001 Jan;27(1):117-20.
Specificity:
The specificity of this antibody has been confirmed by WB. Human, Rat, Mouse, Feline. Predicted to react with other mammals.
Mouse anti-Myelin basic protein (MBP) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of approximately 80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP). MBP is an 'intrinsically unstructured' protein with a high proportion (approximately 75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system. A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational. Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic. The message for myelin basic protein is selectively translocated to the ends of the cell processes. Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model. FUNCTION: Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity). SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Myelin membrane; peripheral membrane protein; cytoplasmic side. Cytoplasmic side of myelin. TISSUE SPECIFICITY: Found in both the central and the peripheral nervous system.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized with 5% trehalose
Host Animal:
Mouse
Species Reactivity:
Bovine,Human,Rat
Immunogen:
Three peptide sequences conserved in higher verterbrate MBP protein.
Applications:
ICC,IHC-Frozen,WB
Clone number:
7G7
Antibody Isotype:
IgG
Application Details:
Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC). IH(P), and Flow Cytometry (~2 ug per10^6 cells). The recommended dilution for WB is 1:5,000-10,0000 and 1:500-1,000 for IC and IH and IH(P). Material should not be over fixed; 2-3 hour post-fixing time is recommended. Long fixations can effect reactivity. In paraffin citrate acid treatment for antigen recovery is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Schwartz, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12440382">Prog Brain Res 137, 401-6 (2002) Hauben, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed/11518733">J Clin Invest 108, 591-9 (Aug, 2001) Yoles, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11356861">J Neurosci 21, 3740-8 (Jun 1, 2001) Hauben, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10964948">J Neurosci 20, 6421-30 (Sep 1, 2000) Harauz, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15219899">Nature 389, 783-4 (1997). Micron 35, 503-42 (2004) Givogri, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10650873">J Neurosci Res 59, 153-9 (Jan 15, 2000) Kim, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9251242">Int J Biochem Cell Biol 29, 743-51 (May, 1997) Kalwy, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7522797">Mol Membr Biol 11, 67-78 (Apr-Jun, 1994) Wajgt, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6198864">Acta Neurol Scand 68, 337-43 (Nov, 1983) Day, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2418054">J Neuroimmunol 10, 289-312 (Feb, 1986) Mikoshiba, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1709079">Comp Biochem Physiol C 98, 51-61 (1991) Brophy, et al., <a class="newA" target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7509522">Trends Neurosci 16, 515-21 (Dec, 1993) Matsuo, A. et al. (1997) Am. J. Pathol. 150(4): 1253-1266
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody stains bands around 21.5 kDa and 18.5 kDa. A suitable control tissue is rat spinal cord or peripheral nerve homogenate. The major isoforms of MBP run as a closely spaced double of 22 kDa and 18 kDa. Human, Rat
Mouse anti-Glial fibrillary acidic protein (GFAP) Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC.
Background Info:
GFAP is a 50 kDa intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes. During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells. GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS, pH 7.4, containing 3% trehalose and 0.05% sodium azide.
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:5,000 is recommended for WB. Human GFAP has a predicted length of 432 residues and a MW of 50 kDa. A dilution of 1:500-1:1,000 is recommended for ICC/IHC. This antibody works well on frozen sections, cells in tissue culture and on formalin fixed histological sections. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Astrocyte; Glial fibrillary acidic protein; GFAP;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Reeves S.A, et al. Proc. Natl. Acad. Sci. U.S.A. 86:5178-5182(1989). 2. Brenner M, et al. Brain Res. Mol. Brain Res. 7:277-286(1990). Isaacs A, et al. Genomics 51:152-154(1998). Ota T, et al. Nat. Genet. 36:40-45(2004). Nielsen A.L, et al. J. Biol. Chem. 277:29983-29991(2002). Singh R, et al. Genomics 82:185-193(2003). Brenner M, et al. Nat. Genet. 27:117-120(2001). Brockmann K, et al. Eur. Neurol. 50:100-105(2003). Stumpf E, et al. Arch. Neurol. 60:1307-1312(2003). Sawaishi Y, et al. Neurology 58:1541-1543(2002). Aoki Y, et al. Neurosci. Lett. 312:71-74(2001).
Specificity:
The specificity of this antibody has been confirmed by WB. Human, Rat, Mouse, Bovine, Porcine. Predicted to react with other mammalian and avian species.
Clone OX-42 recognises the rat equivalent of human CD11b and shares a common epitope with CB11c (integrin apha M and alpha X chains). (PMID:1672643; Tamatani T et al 1991). CD11b is a single-pass type I membrane protein that belongs to the integrin alpha chain family. CD11b is predominantly expressed in monocytes and granulocytes and is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles (Ref: SWISSPROT). CD11b is also frequently used as a microglial marker allowing to distinguish between quiescent and activated microglia based on the intensity of CD11b staining. Moreover the OX-42 monoclonal antibody specifically binds to the CR3 complement (C3bi) receptor found on most monocytes, granulocytes, macrophages, dendritic cells, and microglia. OX-42 antibody inhibits C3bi binding activity. CD11b, also known as integrin alpha M or Mac-1, and is a component of complement receptor 3 (CR3). CD11c, also known as integrin alpha X, and is a component of complement receptor 4 (CR4). Integrin alpha-X/beta-2 is a receptor for fibrinogen. CD11b and CD11c are expressed on immune cells such as macrophages, monocytes, granulocytes, and dendritic cells. OX42 has also been shown to detect microglia in the brain, as well as cells of the liver and epidermis.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS containing no preservatives.
Host Animal:
Mouse
Species Reactivity:
Rat
Immunogen:
Rat peritoneal macrophages, whole cells. (Robinson, AP et al Immunology 1986 57 239-247)
Applications:
ICC,IHC-Paraffin-embedded
Clone number:
OX42, OX-42
Antibody Isotype:
IgG2a, kappa
Application Details:
FC: Flow Cytometry: Unfixed cells preferred, acetone fixed or quickly fixed 1% PLP fixed cells can be used. IH: Immunohistochemical studies of rat fresh frozen tissue sections and paraffin-embedded tissue sections following either periodate-lysine-paraformaldehyde (PLP) fixation, or acetone. Works on very lightly PFA fixed, frozen tissues. (perfusion only 4% PFA 10-15' no post-fix). Epitope can be sensitive to fixation. Dilutions detection method dependent 1:100 to 1:200 recommended. IC: Unfixed preferred, or acetone fixed cells; 5-10', 2% PLP fixed cells, 1-2µg/mL. Dilution is detection method dependent. Immunoprecipitation: use rabbit anti-mouse or anti-mouse IgG beads for capture only. The use of protein A or protein G is not recommended. 1-5µg/mL in restricted volumes. Clone does not work in traditional reduced westerns. Use immunoprecipitation to resolve reactive protein bands. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
CD11b; CD11B; CD11 antigen-like family member B; ITGAM; Integrin beta 2 alpha subunit; CD11c;
Biosensis Brand:
Biosensis®
Conjugate:
Unconjugated
Shelf Life:
12 months after date of receipt (unopened vial).
Use:
For research use only.
References:
Zilka N. et al (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19232747'> J Neuroimmunol. Apr 30;209(1-2):16-25. (activated microglia) Blackbeard J. et al (2007) Quantification of the rat spinal microglial response to peripheral nerve injury as revealed by immunohistochemical image analysis and flow cytometry. https://www.ncbi.nlm.nih.gov/pubmed/17553569'> J Neurosci Methods. Aug 30;164(2):207-17. Eljaschewitsch E. et al (2006) The endocannabinoid anandamide protects neurons during CNS inflammation by induction of MKP-1 in microglial cells http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16387640'> Neuron. Jan 5;49(1):67-79 (activated microglia) 4. Robinson A.P., White T.M. and Mason D.W. (1986) Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=3512425'> Immunology. 1986 Feb;57(2):239-47. (original paper)
Specificity:
Clone OX-42 recognises the rat equivalent of human CD11b and shares a common epitope with CB11c (integrin apha M and alpha X chains). (PMID:1672643; Tamatani T et al 1991). Immunoprecipitates three polypeptides of 160 kDa, 103 kDa and 95 kDa and a fainter band may also be seen at 133 kDa under non-reducing conditions. If the immunoprecipitated proteins are reduced, two major peptides of 163 kDa and 100 kDa and a minor 135 kDa peptide are seen. Mis-information exists concerning reactivity to mouse and human CD11b/c with OX-42 from various vendors. Biosensis has not verified that OX42 reacts with mouse and human, and ONLY recommends the clone only for rat as the original paper and most papers use the OX family of clones on rat.
CD4 is a single-pass type I membrane protein expressed on the surface of T helper/inducer cells, regulatory T cells, monocytes, macrophages, dendritic cells and microglia.
CD4 transfected mouse T-cell hybridoma 3DT followed by CD4+ human T-cell CEM cell line
Applications:
IHC-Frozen,IHC-Paraffin-embedded
Clone number:
CA-4
Antibody Isotype:
IgG
Application Details:
Immunohistochemistry (IHC-Frozen). A concentration of 1.0 µg/mL is recommended to detect CD4 in formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Almolda B. et al. CD4 microglial expression correlates with spontaneous clinical improvement in the acute Lewis rat EAE model. J Neuroimmunol. 2009 Apr 30;209(1-2):65-80. Jordan C.A. et al. Infection of brain microglial cells by human immunodeficiency virus type 1 is CD4 dependent. J Virol. 1991 Feb;65(2):736-42.
Specificity:
The specificity of this antibody has been confirmed by immunohistochemistry against the antigen. Human; rat;
X
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