The Biosensis NGFR/p75ECD RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of human p75 neurotrophin receptor in less than 4 hours in urine, serum, citrate-plasma, CSF and cell culture supernatants only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.Note that capture and detection antibodies have been optimized for increased assay sensitivity, and reagents from previous lots should not be used. This optimized human p75ECD ELISA assay demonstrates an increased sensitivity, lowering the LOD about 8-fold to the previous assay version. It represents the most sensitive NGFR/p75ECD ELISA assay available today, with a detection limit of at least 6 pg/mL, and a quantification range of 7.8 - 500 pg/mL.This ELISA kit consists of a pre-coated mouse monoclonal anti-p75ECD capture antibody, a biotinylated mouse monoclonal anti-p75ECD detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75ECD present in samples and protein standards. A human p75ECD positive control (QC sample) is provided to assure consistent assay performance.This NGFR/p75ECD ELISA kit employs an in-house recombinant human p75ECD-Fc chimera as standard that has been validated against an externally available p75ECD-Fc protein. While there is a current lack of a commercially available, true human p75ECD standard, this ELISA kit allows quantification of human p75ECD as p75ECD-Fc human equivalents. Please note that the antibodies used in this ELISA do not cross-react with mouse p75ECD. Note: For research use only. Not for diagnostic and clinical purposes. Note: For research use only. Not for diagnostic and clinical purposes.
Background Info:
The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.
Product Type:
ELISA
Antibody Type:
Monoclonal-Monoclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C.
Species Reactivity:
Human, Mouse (Negative), Rat (Negative)
Immunogen:
Recombinant human p75 (AA 1-250) -Fc chimera, made in 293 cells
The Biosensis NGFR/p75ECD RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of human p75 neurotrophin receptor in less than 4 hours in urine, serum, citrate-plasma, CSF and cell culture supernatants only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.Note that capture and detection antibodies have been optimized for increased assay sensitivity, and reagents from previous lots should not be used. This optimized human p75ECD ELISA assay demonstrates an increased sensitivity, lowering the LOD about 8-fold to the previous assay version. It represents the most sensitive NGFR/p75ECD ELISA assay available today, with a detection limit of at least 6 pg/mL, and a quantification range of 7.8 - 500 pg/mL.This ELISA kit consists of a pre-coated mouse monoclonal anti-p75ECD capture antibody, a biotinylated mouse monoclonal anti-p75ECD detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75ECD present in samples and protein standards. A human p75ECD positive control (QC sample) is provided to assure consistent assay performance.This NGFR/p75ECD ELISA kit employs an in-house recombinant human p75ECD-Fc chimera as standard that has been validated against an externally available p75ECD-Fc protein. While there is a current lack of a commercially available, true human p75ECD standard, this ELISA kit allows quantification of human p75ECD as p75ECD-Fc human equivalents. Please note that the antibodies used in this ELISA do not cross-react with mouse p75ECD. Note: For research use only. Not for diagnostic and clinical purposes.
The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.
Background Info:
The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.
Product Type:
ELISA
Antibody Type:
Monoclonal-Monoclonal
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C.
Species Reactivity:
Human, Mouse (Negative), Rat (Negative)
Immunogen:
Recombinant human p75 (AA 1-250) -Fc chimera, made in 293 cells
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. This antibody binds primarily to the phosphorylated axonal forms of NF-H.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with 5% trehalose
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Chicken
Species Reactivity:
Hu, Rat, Ms, Fel, Chk, Other Mammalian (Predicted)
Western Blotting (WB), Immunocytochemistry (IC) and ELISA. Suggested dilution for WB is 1:100,000-1,000,000. Suggested dilution for IC is 1:50,000-100,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Accession Number:
P12036 NFH_HUMAN;
Reconstitution:
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
This antibody reacts with phosphorylated NF-H and is seen as a band of approx 200 kDa in WB. Refer to publication by Shaw et al (2005) for the use of this antibody in an ELISA to detect NF-H. | Hu, Rat, Ms, Fel, Chk. Predicted to react with other mammalian tissues due to sequence homology.
References:
1. Jarjour A.A. et al (2007) Maintenance of axo-oligodendroglial paranodal junctions requires DCC and netrin-1. J Neurosci. 2008 Oct 22;28(43):11003-14.2. Pearse D.D. et al (2007) Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery. Glia. 2007 Jul;55(9):976-1000.3. Shaw G. et al (2005) Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury. Biochem Biophys Res Commun. 2005 Nov 4;336(4):1268-77.
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with 5% trehalose
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Chicken
Species Reactivity:
Hu, Rat, Ms, Fel, Chk, Other Mammalian (Predicted)
Immunogen:
Purified porcine NF-L from spinal cord and recombinant NF-L.
Western Blotting (WB), Immunocytochemistry (IC), Immunohistochemistry (IHC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:5,000 is recommended for IC, IHC. IHC: 4% Frozen tissues, permeabilized; IH(P): capable, HEIR treatment typically necessary; Chicken IgY can bind non-specifically to tissues, special chicken block solutions available elsewhere are recommended if used on tissues. Dilutions 1:5000 or greater; optimization on time, method required for best results Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
The specificity of this antibody has been confirmed by IC. | Hu, Rat, Ms, Fel, Chk. Predicted to react with other mammalian tissues due to sequence homology.
References:
1. Rangaraju S. et al (2009) Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging. Aging Cell. 2009 Apr;8(2):178-91.
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized with 5% trehalose
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Chicken
Species Reactivity:
Hu, Rat, Ms, Fel, Chk, Other Mammalian (Predicted)
Immunogen:
Recombinant fusion protein containing the extreme C-terminal segment of rat NF-M.
Western Blotting (WB), Immunocytochemistry (IC), Immunohistochemistry (IHC). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:3,000 is recommended for IC, IHC. IHC: 4% Frozen tissues, permeabilized; IH(P): capable, HEIR treatment typically necessary; Chicken IgY can bind non-specifically to tissues, special chicken block solutions available elsewhere are recommended if used on tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm;
Accession Number:
P12839 NFM_RAT;
Reconstitution:
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
Specifically recognizes the medium neurofilament subunit NF-L in WB. Band appears at ~145 kDa in WB from rodent and ~160 kDa for human and bovine WB. | Hu, Rat, Ms, Fel, Chk. Predicted to react with other mammalian tissues due to sequence homology.
References:
1. Jarjour A.A. et al (2007) Maintenance of axo-oligodendroglial paranodal junctions requires DCC and netrin-1. J Neurosci. 2008 Oct 22;28(43):11003-14.2. Rangaraju S. et al (2009) Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging. Aging Cell. 2009 Apr;8(2):178-91.3. Pearse D.D. et al (2007) Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery. Glia. 2007 Jul;55(9):976-1000.4. Shaw G. et al (2004) Characterization of the bovine neurofilament NF-M protein and cDNA sequence, and identification of in vitro and in vivo calpain cleavage sites. Biochem Biophys Res Commun. 2004 Dec 10;325(2):619-25.
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Storage Temp:
Short term storage at 2-8 °C for one week. At -20 °C as an undiluted liquid for up to 12 months.
Host Animal:
Chicken
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Mixture of two human GDNF peptides (101-118 and 199-211 aa). Both peptides are highly conserved in human and mouse.
ELISA, WB and IHC. WB suggested dilution of 1:500-1:2,000. IHC suggested dilution of 1:50-1:500. Biosensis recommends that the optimal working dilution should be determined by the end user.
CNTF is a survival promoting factor for different types of neurons in vitro and in vivo. The essential structural features for the biological function of human CNTF were investigated by Thier, M. et al. They showed that deletion of 14 N-terminal and 18 C-terminal amino acids significantly increased bioactivity compared to wild-type CNTF. FUNCTION: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Nervous system. PHARMACEUTICAL: CNTF is being tested under the name Axokine by Regeneron Pharmaceuticals for treatment of human motor neuron diseases, such as amyotrophic lateral sclerosis (ALS). As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. SIMILARITY: Belongs to the CNTF family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Liquid. PBS with 0.02% Sodium Azide
Storage Temp:
Short term storage at 2-8 °C for one week. At -20 °C as an undiluted liquid for up to 12 months.
Host Animal:
Chicken
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Mixture of two peptides from human CNTF (11-25 and 186-200 aa). Both peptides are highly conserved among human and mouse.
WB and ELISA at a suggested dilution of 1:500 to 1:2,000. A titration between 1:50 to 1:500 is suggested for IHC. Biosensis recommends that the optimal working dilution should be determined by the end user.
Alternative Names:
Ciliary neurotrophic factor;
Accession Number:
P26441 CNTF_HUMAN;
Shelf Life:
12 months after purchase
Specificity:
CNTF, Cross reactivity to other species is expected
Monoclonal antibody MC192 against the rat low affinity nerve growth factor receptor (p75NTR) is derived from the fusion of Sp2/0-Ag 14 myeloma cells with mouse immune splenocytes. MC192 monoclonal antibody was originally generated by Chandlers et al. p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. MC192 recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat. MC192 antibody may be used for immunocytochemical localisation of rat cells expressing p75NTR, ELISA and western blot. This antibody has also been used for the construction of the MC192-saporin immunotoxin for specific elimination of neuronal populations in basal forebrain cholinergic neurons to generate an animal model for Alzheimer's disease. Using Flow Cytometry, this antibody has frequently been employed for panning to isolate p75NTR-expressing rat cells. MC192 has a potential use as the ligand for gene delivery into p75NTR-expressing rat cells via a receptor-mediated mechanism. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1 (By similarity). Interacts with BEX1. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. DOMAIN: Death domain is responsible for interaction with RANBP9. PTM: N- and O-glycosylated. PTM: Phosphorylated on serine residues. SIMILARITY: Contains 1 death domain. SIMILARITY: Contains 4 TNFR-Cys repeats.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
The MC192 is supplied in lyophilised form from Protein G-purified hybridoma cell culture supernatants. The lyophilised antibody is stable when stored at 2-8 °C or -20 °C. After reconstitution undiluted aliquots should be kept at -20 °C for up to six months. For additional stability Glycerol (1:1) may be added after reconstitution. Repetitive freeze/thaw cycle should be avoided.
IH (likely fixed), ELISA, WB, Flow Cytometry (2 µg per 10^6 cells) IP (non-reducing conditions only!; do not use reducing agents such as DTT or beta-mercaptoethanol), Traditional formalin fixed paraffin embedded immunohistochemistry is NOT recommended with MC192. Motor neuron isolation, Gene/Toxin Delivery to rat sensory/motor neurons. A working solution of 1-2 µg/mL was determined by immunohistochemical staining on 4% paraformaldehyde fixed, or alcohol fixed rat spinal cord and brain. For non-denatured WB, 1-5 ug/mL was found to be suitable with suitable controls (PC12 lysate). ELISA: detection only, 1-5ug/mL has been suggested in literature.Immunoprecipitation: 5 µg/mL, > 0.5% triton X-100 buffer/500ug/lysate; PC12 positive control strong suggested.MC192 is not suitable as a blocking agent, although it has been incorrectly used for this purpose in many published works. The antibody was generated specifically by screening for monoclonals that had the ability to ENHANCE the binding of NGF, the natural ligand for p75. Therefore, this antibody is particularly unusual. The full details can be found in the original paper, which is listed on our datasheet (see Chandler et al, 1984). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity:
MC192 is specific only for RAT NGFR, no reactivity to Human or Mouse NGFR has been reported | This monoclonal antibody has been tested for immunohistochemical localisation of p75NTR-expressing rat cells in the spinal cord and brain. This monoclonal antibody does not cross react with p75NTR-expressing cells in other species.
References:
Riffault B, Kourdougli N, Dumon C, Ferrand N, Buhler E, Schaller F, Chambon C, Rivera C, Gaiarsa JL, Porcher C (2016) Pro-Brain-Derived Neurotrophic Factor (proBDNF)-Mediated p75NTR Activation Promotes Depolarizing Actions of GABA and Increases Susceptibility to Epileptic Seizures. Cereb. Cortex [Epub ahead of print]. Application: Western Blot; Species: RatBrandli A, Johnstone DM, Stone J (2016) Remote Ischemic Preconditioning Protects Retinal Photoreceptors: Evidence From a Rat Model of Light-Induced Photoreceptor Degeneration. Invest Ophthalmol Vis Sci. 57(13):5302-13 Application: Western Blot, IHC; Species: RatRiffault B, Medina I, Dumon C, Thalman C, Ferrand N, Friedel P, Gaiarsa JL, Porcher C. (2014) "Pro-Brain-Derived Neurotrophic Factor Inhibits GABAergic Neurotransmission by Activating Endocytosis and Repression of GABAA Receptors." J. Neurosci. 34(40):13516-34 Application: Western Blot,Neuronal cells and hippocampi; Species: RatKalincik T et al (2011) Selected changes in spinal cord morphology after T4 transection and olfactory ensheathing cell transplantation. Auton Neurosci. 158(1-2):31-8 Application: IF; Species: RatWu A et al (2011) Delayed olfactory ensheathing cell transplants reduce nociception after dorsal root injury. Exp Neurol. 229(1):143-57 Application: IF; Species: RatDavies A et al (2010) The alpha2delta subunits of voltage-gated calcium channels form GPI-anchored proteins, a post translational modification essential for function Proc Natl Acad Sci U S A. Jan 26;107(4):1654-9 Kalincik T et al (2010) Olfactory ensheathing cells reduce duration of autonomic dysreflexia in rats with high spinal cord injury. Auton Neurosci. 154 (1-2):20-9 Application: IHC; Species: RatWilson-Gerwing T.D. et al (2009) J Comp Neurol. 2009 Sep 1;516(1):49-58 Feron F et al (2008) Neurotrophin expression in the adult olfactory epithelium. Brain Res. 1196:13-21 Application: IHC; Species: RatBianco JI et al (2004) Neurotrophin 3 promotes purification and proliferation of olfactory ensheathing cells from human nose. Glia. 45(2):111-23 Application: IHC, IF; Species: RatEyles D et al (2003) Neuroscience. 2003;118(3):641-53. Application: IHC; Species: RatLu J et al (2001) Transplantation of nasal olfactory tissue promotes partial recovery in paraplegic adult rats. Brain Res. 889(1-2):344-57 Application: IF; Species: Rat
Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) due to its molecular mass and its ability to bind at low affinity not only NGF (see 162030), but also other neurotrophins, including brain-derived neurotrophic factor (BDNF; 113505), neurotrophin-3 (NTF3; 162660), and neurotrophin-4/5 (NTF5; 162662). At the time of its discovery, NGFR was considered a unique type of protein. Subsequently, however, a large superfamily of tumor necrosis factor receptors were found to share the overall structure of NGFR (4 extracellular ligand-binding, cysteine-rich repeats, or CRs, and signaling through association with, or disassociation from, cytoplasmic interactors). The identification of this superfamily helped elucidate some of the biologic functions of NGFR, including its ultimate involvement in the nuclear factor kappa-B (NFKB; see 164011) and apoptosis pathways. As a monomer, NGFR binds NGF with low affinity. Higher affinity binding is achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA (NTRK1; 191315), TRKB (NTRK2; 600456), and TRKC (NTRK3; 191316). TRKA, TRKB, and TRKC are specific for or 'preferred by' NGF, NTF5 and BDNF, and NTF3, respectively (Ip et al., 1993). NTF3 also binds to TRKA and TRKB, but with significantly lower affinity
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Human, cat, dog, Pig, rabbit, sheep, Mouse (Negative), Rat (Negative)
Immunogen:
The p75NTR antibody was derived from immunization of mice with human WM245 melanoma cells.
Immunohistochemistry, immunofluorescence, flow cytometry. Suggested working dilutions: For Immunohistochemistry a concentration of 2 µg/mL is recommended. Antibody not appropriate for Western Blot. For FACS a concentration of 20 µg/mL is recommended. At least 1 in 5000 dilution is recommended for 1 site ELISAs. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Reconstitute with the addition of 100 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity:
This antibody recognises p75NTR (low affinity neurotrophin receptor) | Reacts with human, cat, dog, pig, rabbit and sheep. Does not react with rat or mouse.
References:
1. Liu W. et al (2012) Distribution of P75 neurotrophin receptor in adult human cochlea-an immunohistochemical study. Cell Tissue Res. 2012 Mar 31.2. Inoue K. et al. (2009) Differential expression of stem-cell-associated markers in human hair follicle epithelial cells. Lab Invest. 2009 Aug;89(8):844-56. 3. Ariga M. et al. (2008) Functional role of sortilin in myogenesis and development of insulin-responsive glucose transport system in C2C12 myocytes J Biol Chem. 2008 Apr 11;283(15):10208-20 4. Rogers mL et al (2010) ProNGF mediates death of Natural Killer cells through activation of the p75NTR-sortilin complex. J Neuroimmunol. 2010 Sep 14;226(1-2):93-103.5. Jiao et al. Differentiation defect in neural crest-derived smooth muscle cells in patients with aortopathy associated with bicuspid aortic valves. EBioMedicine (2016) 10:282-90.
Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS and then lyophilized. Purity was analysed using electrophoresis, 4-12% Bis Tris Gel (Invitrogen).
Monoclonal antibody MC192 against the rat low affinity nerve growth factor receptor (p75NTR) is derived from the fusion of Sp2/0-Ag 14 myeloma cells with mouse immune splenocytes. MC192 monoclonal antibody was originally generated by Chandlers et al. p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. MC192 has a potential use as the ligand for gene delivery into p75NTR-expressing rat cells via a receptor-mediated mechanism. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
The antibody conjugate can be stored at 4 °C for up to 4 months with the addition of appropriate antibacterial agent.
Host Animal:
Mouse
Species Reactivity:
Rat, Human (Negative), Mouse (Negative)
Immunogen:
Rat p75NTR
Applications:
Not Suitable for WB; IHC, Frozen; IF, Frozen; IC; FC;
The FITC version of MC192 is primarily targeted for FACS or IF applications on live or lightly fixed cells.Immunofluorescence (1-2 ug/mL), flow cytometry (20 ug/mL) NGF receptor p75 dynamics, retrograde transport studies, study of intracellular trafficking. (10-20ug/mL). ELISA (1 Suggested working dilutions: The antibody is not appropriate for Western Blots. Antibody will not work in traditional formalin fixed tissues. The recommended concentration for FACS is 20 µg/mL and at least 1 in 5000 dilution is recommended for 1 site ELISA. Optimal working dilution should be determined by the end user. MC192 is not suitable as a blocking agent, although it has been incorrectly used for this purpose in many published works. The antibody was generated specifically by screening for monoclonals that had the ability to ENHANCE the binding of NGF, the natural ligand for p75. Therefore, this antibody is particularly unusual. The full details can be found in the original paper, which is listed on our datasheet (see Chandler et al, 1984). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
MC192 recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat. | Reacts with rat. Does not react with mouse or human p75 NGFR
References:
1. Davies A. et al (2010) The alpha2delta subunits of voltage-gated calcium channels form GPI-anchored proteins, a post translational modification essential for function Proc Natl Acad Sci U S A. Jan 26;107(4):1654-9
M-1763-100-FT, Non-specific Control IgG, clone X63 (Monoclonal) - FITC
Purity:
Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The antibody was then conjugated to Fluorescein isomer 1 (FITC, Sigma). A minimum fluorescein: protein ratio of 3:1 is guaranteed. The conjugate was purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) due to its molecular mass and its ability to bind at low affinity not only NGF (see 162030), but also other neurotrophins, including brain-derived neurotrophic factor (BDNF; 113505), neurotrophin-3 (NTF3; 162660), and neurotrophin-4/5 (NTF5; 162662). At the time of its discovery, NGFR was considered a unique type of protein. Subsequently, however, a large superfamily of tumor necrosis factor receptors were found to share the overall structure of NGFR (4 extracellular ligand-binding, cysteine-rich repeats, or CRs, and signaling through association with, or disassociation from, cytoplasmic interactors). The identification of this superfamily helped elucidate some of the biologic functions of NGFR, including its ultimate involvement in the nuclear factor kappa-B (NFKB; see 164011) and apoptosis pathways. As a monomer, NGFR binds NGF with low affinity. Higher affinity binding is achieved by association with higher molecular mass, low-affinity neurotrophin receptors, namely the tropomyosin receptor kinases, TRKA (NTRK1; 191315), TRKB (NTRK2; 600456), and TRKC (NTRK3; 191316). TRKA, TRKB, and TRKC are specific for or 'preferred by' NGF, NTF5 and BDNF, and NTF3, respectively. NTF3 also binds to TRKA and TRKB, but with significantly lower affinity.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid. 10mM Tris, 50mM NaCl
Storage Temp:
The antibody conjugate can be stored at 4 ºC for up to 4 months with the addition of appropriate antibacterial agent.
Host Animal:
Mouse
Species Reactivity:
Human, cat, dog, Pig, rabbit, sheep, Mouse (Negative), Rat (Negative)
Immunogen:
The p75NTR antibody was derived from immunization of mice with human WM245 melanoma cells.
Applications:
Not Suitable for WB; IHC, Frozen; IF, Frozen; IC; FC;
This antibody is recommended for use in immunohistochemistry, immunofluorescence, flow cytometry and NGF receptor p75 dynamics. For immunohistochemistry a concentration of 2 µg/mL is recommended. Not appropriate for Western Blots. For FACS a concentration of 20 µg/mL is recommended and for 1 site ELISA at least a 1 in 5000 dilution. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
This antibody recognises p75NTR (low affinity neurotrophin receptor) | Reacts with human, cat, dog, pig, rabbit and sheep. Does not react with rat or mouse.
M-1763-100-FT, Non-specific Control IgG, clone X63 (Monoclonal) - FITC
Purity:
Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The antibody was then conjugated to Fluorescein isomer 1 (FITC, Sigma). A minimum fluorescein: protein ratio of 3:1 is guaranteed. The conjugate was purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
Monoclonal antibody MC192 against the rat low affinity nerve growth factor receptor (p75NTR) is derived from the fusion of Sp2/0-Ag 14 myeloma cells with mouse immune splenocytes. MC192 monoclonal antibody was originally generated by Chandlers et al. p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. MC192 has a potential use as the ligand for gene delivery into p75NTR-expressing rat cells via a receptor-mediated mechanism. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1 (By similarity).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid
Storage Temp:
The antibody conjugate can be stored at 4 ºC for up to 4 months with the addition of appropriate antibacterial agent.
IF: live or lightly fixed cells or tissues (acetone or 4% PFA): 2-5ug/mL. Not suitable for western blots; not suitable for IH on formalin fixed tissues. FACS (20ug/mL) is recommended, unfixed cells.
MC192 recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat and does not react with human or mouse NGFR. | Reacts with rat. Does not react with mouse or human NGFR
References:
1. Davies A. et al (2010) The alpha2delta subunits of voltage-gated calcium channels form GPI-anchored proteins, a post translational modification essential for function Proc Natl Acad Sci U S A. Jan 26;107(4):1654-9
Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The IgG was then conjugated to ATTO 488 (ATTO TEC) and purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized with 5% trehalose
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Chicken, Rat, Other Mammals (Predicted)
Immunogen:
Full length native protein (purified) from Pig spinal cord.
Western Blotting (WB), Immunocytochemistry (IC) and Flow Cytometry (2 ug/10^6 cells). Suggested dilution for WB of 1:5,000-10,000. This antibody recognises NF-H in frozen sections, tissue culture and in formalin-fixed sections. Suggested dilution for IC is 1:500-1,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Accession Number:
P12036 NFH_HUMAN;
Reconstitution:
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody recognises phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related KSP sequences found in subunit NF-M. | Chicken, Rat. Predicted to react with mammals due to sequence homology.
References:
1. Boylan K. et al (2009) Immunoreactivity of the phosphorylated axonal neurofilament H subunit (pNF-H) in blood of ALS model rodents and ALS patients: evaluation of blood pNF-H as a potential ALS biomarker. J Neurochem. 2009 Dec;111(5):1182-91.2. Rangaraju S. et al (2009) Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging. Aging Cell. 2009 Apr;8(2):178-91.
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized with 5% trehalose
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Hu, Rat, Ms, Fel, Bov, Por, Chk
Immunogen:
A preparation of enzymatically dephosphorylated pig neurofilaments including NF-L, NF-M and NF-H. Screening was by ELISA on the immungen followed by immunofluorescence microscopy. The epitope for this antibody is not in the N-terminus but its exact location is not known. It is thought to be somewhere within the central alpha helical "rod" region of the molecule.
Western Blotting (WB), Immunocytochemistry (IC), Immunohistochemistry (IH) and Flow Cytometry (2 ug per 10^6 cells). A dilution of 1:5,000 - 1:10,000 is recommended for WB. A dilution of 1:100 - 1:500 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized with 5% trehalose
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Hu, Rat, Ms, Fel, Bov, Por, Chk
Immunogen:
Raised against a recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli. The epitope is localized to within the last 56 amino acids at the extreme C-terminus of rat NF-M, the so-called KE segment which is highly conserved between NF-M molecules from different species.
Western Blotting (WB), Immunocytochemistry (IC), Immunohistochemistry (IH) and Flow Cytometry. A dilution of 1:2,000 - 1:10,000 is recommended for WB. A dilution of 1:1,000 - 1:5,000 is recommended for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm;
Accession Number:
P12839 NFM_RAT;
Reconstitution:
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
Specifically recognizes the medium neurofilament subunit NF-L in WB. | Hu, Rat, Ms, Fel, Bov, Por, Chk
References:
1. Felitsyn N. et al (2008) The heme precursor delta-aminolevulinate blocks peripheral myelin formation. J Neurochem. 2008 Sep;106(5):2068-79.
Peripherin is a class-III neuronal intermediate filament protein found in certain classes of neuron, most of which are located in the peripheral nervous system.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS, pH 7.4, containing 3% trehalose, without preservatives.
Storage Temp:
Store lyophilised, unopened vial at 2- 8 °C or lower. After reconstitution, prepare aliquots and store at -20 °C to -80 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Human, mouse, feline, Other Mammalian (Predicted)
Immunogen:
Recombinant full length rat Peripherin protein expressed in and purified from E.coli
Western Blotting (WB), Immunocytochemistry (IC) and Immunohistochemistry (IH). A concentration of 0.5 - 2 µg/mL is recommended for WB. A concentration of 1-5 µg/mL is recommended for IC and IH. This antibody performs well on aldehyde fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Peripherin; Prph; Prph1;
Accession Number:
P21807 PERI_RAT;
Reconstitution:
Reconstitute with 100 µL sterile-filtered, ultrapure water, to achieve an antibody concentration of 1 mg/mL. Centrifuge briefly to remove any insoluble material.
Shelf Life:
12 months after purchase (unopened vial).
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody detects ~57 kDa Peripherin protein. | Human, mouse, feline. Predicted to react with other mammalian tissue.
References:
Sekerková G. et al (2008) Espin actin-cytoskeletal proteins are in rat type I spiral ganglion neurons and include splice-isoforms with a functional nuclear localization signal. J Comp Neurol. 2008 Aug 20;509(6):661-76.
Lyophilized from a solution containing PBS buffer pH 7.4 with 3% trehalose, without preservatives.
Storage Temp:
After reconstitution divide into aliquots and store at -20 ºC for a higher stability. Antibody contains no preservatives. Store at 4 ºC with an appropriate antibacterial agent. Use sterile methods. Highest purity Glycerol (1:1) may be added for an additional stability when stored at refrigerated or freezing temperatures. Avoid repetitive freeze/thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Rat, Mouse (Predicted)
Immunogen:
A synthetic peptide (C-EDYPDQFDDVMD, aa: 55-66) of human proGDNF protein has been used as the immunogen. The sequence is located on the pro-domain of the proGDNF full-length protein and is homologous with mouse and rat form of proGDNF.
Flow Cytometry (2 µg/ 106 cells). Immunocytochemistry (1-2 µg/mL). Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Spin vial briefly before opening. Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Shelf Life:
12 months after purchase if unopened
Specificity:
Human | Rat. Antibody is expected to detect mouse proGDNF protein due to 100% amino acid sequence homology.
Protein S100 beta belongs to the S100 family of proteins containing 2 EF-hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Among brain cells, S100 beta is often used as a marker for astrocytes. The 2 subunits of S100 protein are the products of separate genes. The beta subunit is present in all S100 positive cells and tumours whereas the alpha subunit is detectable only in specific tissues such as heart, skeletal muscle and brain. The S100 protein is a homodimer composed of two alpha subunits (S100a) or two beta subunits (S100b) or as a heterodimer with one alpha and beta subunit (S100ab or S100ao). This antibody reacts specifically with the beta subunit.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from mouse ascites fluid containing 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative.
Storage Temp:
12 months after purchase at -20 °C (lyophilized formulations).After reconstitution, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Human, rabbit, Rat, Horse (Predicted), Monkey (Predicted)
Immunohistochemistry (IHC). A concentration of 1.0-2.0 µg/mL is recommended to detect S100 beta subunit in paraffin embedded tissues. Antigen retrieval by heat is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
S100B; Calcium binding protein B1;
Accession Number:
P02638 S100B_BOVIN
Reconstitution:
Reconstitute in 1 mL of PBS buffer to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase.
Specificity:
The specificity of this antibody has been confirmed by IHC against the antigen. | Human, rabbit and rat. May cross-react with horse and monkey S100-beta based on amino acid sequence similarities.
p75NTR (CD271) was originally discovered as a low affinity nerve growth factor receptor (NGFR). Later it was found that it was the receptor for all neurotrophins, including NGF, BDNF, NT3 and NT4/5. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. Recent research proposes the extracellular domain of p75NTR as a biomarker for monitoring the progression of motor neuron disease (MND), also known as Amyotrophic Lateral Sclerosis (ALS) or Lou Gehrig's Disease. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS buffer pH 7.2-7.6 with 3% trehalose, without preservatives.
Storage Temp:
Store lyophilised antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.
Flow Cytometry: 5-20 ug/mL. Western Blotting: 0.5-2.0 ug/mL, non-reducing conditions only (no DTT or beta-mercaptoethanol). Immunoprecipitation: lysate dependent. 10 ug per 200-500 ug total protein. Immunopanning: 1-5 ug/mL. Immunocytochemistry: 1-5 ug/mL. Staining is strongest in non-fixed cells, light fixation is tolerable. Immunohistochemistry: fresh, acetone fixed sections only, epitope is fixation sensitive. Not suitable in formalin-fixed, paraffin (FFPE) embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Spin vial briefly before opening. Reconstitute in 100 µL sterile water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Shelf Life:
12 months after purchase if unopened.
Specificity:
Human, reacts with human, mouse and rat. Cross-reactivity with other species not tested but expected.This antibody is specific for NGFR/p75NTR as demonstRated by western blotting and immunprecipitation. The antibody recognizes extracellular p75NTR under non-reducing conditions.
M-1818-100, Mouse monoclonal antibody to human NGFR/p75NTR [8J2]: IgG (100 µg);M-1819-50-FT, Mouse monoclonal antibody to human NGFR/p75NTR [8J2] - FITC
p75NTR (CD271) was originally discovered as a low affinity nerve growth factor receptor (NGFR). Later it was found that it was the receptor for all neurotrophins, including NGF, BDNF, NT3 and NT4/5. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. Recent research proposes the extracellular domain of p75NTR as a biomarker for monitoring the progression of motor neuron disease (MND), also known as Amyotrophic Lateral Sclerosis (ALS) or Lou Gehrig's Disease. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from a solution containing PBS buffer pH 7.2-7.6 with 3% trehalose, without preservatives.
Storage Temp:
Store lyophilised antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.
Flow Cytometry: 5-20 ug/mL. Western Blotting: 0.5-2.0 ug/mL, non-reducing conditions only (no DTT or beta-mercaptoethanol). Immunoprecipitation: lysate dependent. 10 ug per 200-500 ug total protein. Immunopanning: 1-5 ug/mL. Immunocytochemistry: 1-5 ug/mL. Staining is strongest in non-fixed cells, light fixation is tolerable. Immunohistochemistry: fresh, acetone fixed sections only, epitope is fixation sensitive. Not suitable in formalin-fixed, paraffin (FFPE) embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Spin vial briefly before opening. Reconstitute in 20 µL sterile water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Shelf Life:
12 months after purchase if unopened.
Specificity:
Human, reacts with human, mouse and rat. Cross-reactivity with other species not tested but expected.This antibody is specific for NGFR/p75NTR as demonstRated by western blotting and immunprecipitation. The antibody recognizes extracellular p75NTR under non-reducing conditions.
M-1818-100, Mouse monoclonal antibody to human NGFR/p75NTR [8J2]: IgG (100 µg);M-1819-50-FT, Mouse monoclonal antibody to human NGFR/p75NTR [8J2] - FITC
p75NTR (CD271) was originally discovered as a low affinity nerve growth factor receptor (NGFR). Later it was found that it was the receptor for all neurotrophins, including NGF, BDNF, NT3 and NT4/5. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. Recent research proposes the extracellular domain of p75NTR as a biomarker for monitoring the progression of motor neuron disease (MND), also known as Amyotrophic Lateral Sclerosis (ALS) or Lou Gehrig's Disease. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid antibody (1 mg/mL) in PBS, pH 7.2-7.6, without preservative.
Storage Temp:
Liquid antibody is shipped cold. Upon arrival, spin vial briefly, divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.
Immunocytochemistry: 1-5 µg/mL. Light fixation only, or unfixed works best. Epitope is sensitive to fixation. Flow Cytometry: 5-20 ug/mL. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human, reacts with human, mouse and rat. Cross-reactivity with other species not tested but expected.This antibody is specific for NGFR/p75NTR as demonstRated by western blotting and immunprecipitation. The antibody recognizes extracellular p75NTR under non-reducing conditions.
Related Products:
M-1818-100, Mouse monoclonal antibody to human NGFR/p75NTR [8J2]: IgG (100 µg)
Purity:
Protein A purified IgG was labelled with FITC and free dye removed by gel filtration.
Conjugate:
Fluorescein (FITC). Typical Fluorophore/Protein (F/P) - ratio is 3-10.
p75NTR (CD271) was originally discovered as a low affinity nerve growth factor receptor (NGFR). Later it was found that it was the receptor for all neurotrophins, including NGF, BDNF, NT3 and NT4/5. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. Recent research proposes the extracellular domain of p75NTR as a biomarker for monitoring the progression of motor neuron disease (MND), also known as Amyotrophic Lateral Sclerosis (ALS) or Lou Gehrig's Disease. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Liquid antibody (1 mg/mL) in PBS, pH 7.2-7.6, without preservative.
Storage Temp:
Liquid antibody is shipped cold. Upon arrival, spin vial briefly, divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.
Immunocytochemistry: 1-5 µg/mL. Light fixation only, or unfixed works best. Epitope is sensitive to fixation. Flow Cytometry: 5-20 ug/mL. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Human, reacts with human, mouse and rat. Cross-reactivity with other species not tested but expected.This antibody is specific for NGFR/p75NTR as demonstRated by western blotting and immunprecipitation. The antibody recognizes extracellular p75NTR under non-reducing conditions.
Related Products:
M-1818-100, Mouse monoclonal antibody to human NGFR/p75NTR [8J2]: IgG (100 µg)
Purity:
Protein A purified IgG was labelled with ATTO 488 and free dye removed by gel filtration.
Conjugate:
ATTO 488. Typical Fluorophore/Protein (F/P) - ratio is 3-10.
Dynactin subunit 1 (DCTN1) is part of a large macromolecular complex of at least 10 components. It is required for cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. Dynactin subunit 1 binds directly to microtubules and to cytoplasmic dynein. It is expressed in the brain and localises to the cytoplasm and the cytoskeleton. It belongs to the dynactin 150 kDa subunit family and contains one CAP-Gly domain. Abnormalities in DCTN1 cause progressive lower motor neuron disease (PLMND).
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from PBS pH 7.2
Storage Temp:
After reconstitution keep aliquots at -20 ºC for higher stability. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Human
Immunogen:
Partial sequence recombinant protein of human dynactin subunit 1 (amino acids 1082-1270) with GST tag
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. This antibody recognises both phosphorylated and dephosphorylated forms of NF200/NF-H.
Product Type:
Antibody
Antibody Type:
Monoclonal
Format:
Lyophilized from 1.2% sodium acetate, 2mg BSA, 0.01mg NaN3
Storage Temp:
At least 12 months after purchase at 2 - 4 °C (lyophilized formulations). After reconstitution, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
C-terminal segment of enzymatically dephosphorylated pig Neurofilament Heavy (NF200).
Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.50 µg/mL is recommended for WB. Human NF200 has a predicted length of 1026 residues and MW of 112 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect NF200 in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Accession Number:
P12036 NFH_HUMAN; P12037 NFH_PIG
Reconstitution:
Reconstitute in 1 mL of PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase.
Specificity:
The specificity of this antibody has been confirmed by WB and IHC against the antigen. | Human; mouse; rat;
References:
Santos J et al., (2017) "Proteomic Analysis of Human Adipose Derived Stem Cells during Small Molecule Chemical Stimulated Pre-neuronal Differentiation." Int J Stem Cells. 2017; 10(2):193-217. Application: WB. Species: Human
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
WB and dot blot. Recommended to be used at a dilution of 1:1000 to 1:3000 for Western blot. The predicted molecular weight of the GDNF monomer is 11.6 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. DOMAIN: Death domain is responsible for interaction with RANBP9. PTM: N- and O-glycosylated. PTM: O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. PTM: Phosphorylated on serine residues. SIMILARITY: Contains 1 death domain. SIMILARITY: Contains 4 TNFR-Cys repeats.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
IHC, immunofluorescence. Recommended to be used at a dilution of 1:500 to 1:2000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
CNTF is a survival promoting factor for different types of neurons in vitro and in vivo. The essential structural features for the biological function of human CNTF were investigated by Thier, M. et al. They showed that deletion of 14 N-terminal and 18 C-terminal amino acids significantly increased bioactivity compared to wild-type CNTF. FUNCTION: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Nervous system. PHARMACEUTICAL: CNTF is being tested under the name Axokine by Regeneron Pharmaceuticals for treatment of human motor neuron diseases, such as amyotrophic lateral sclerosis (ALS). As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. SIMILARITY: Belongs to the CNTF family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
WB. A dilution of 1:500 to 1: 4000 is recommended for western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Ciliary neurotrophic factor
Accession Number:
CNTF_HUMAN
Reconstitution:
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
This antibody specifically detects CNTF shown by western blot. | This antiserum to known to react with rat, mouse and human CNTF protein.
FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. CATALYTIC ACTIVITY: 2 superoxide + 2 H+ = O2 + H2O2. COFACTOR: Binds 1 copper ion per subunit. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. DISEASE: Defects in SOD1 are the cause of familial amyotrophic lateral sclerosis (FALS); also called amyotrophic lateral sclerosis 1 (ALS1 or ALS). ALS is a degenerative disorder of motorneurons in the cortex, brainstem and spinal cord. ALS is characterized by muscular weakness and atrophy beginning in the hands and spreading to the forearms and legs. Muscle fasciculations are commonly visible. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. ALS is sometimes referred to as Lou Gehrig disease after the famous American baseball player who was diagnosed with the disorder. FALS, the familial form of ALS, accounts for about 10% of the cases and is transmitted in an autosomal dominant manner. The mean age at onset of FALS is 45 years. MISCELLANEOUS: Zinc binding promotes dimerization. SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
human
Immunogen:
A synthetic peptide (ESNGPVK VWGSIK) as part of human superoxide dismutase (SOD1) protein (aa: 24-36) conjugated to diphtheria toxoid
WB. A dilution of 1:500 to 1:1000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Superoxide dismutase [Cu-Zn]; SOD1
Accession Number:
SODC_HUMAN
Reconstitution:
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity:
Specificity was confirmed by western blot detecting human superoxide dismutase (SOD1). | This antiserum is known to react with human superoxide dismutase (SOD1).
FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. CATALYTIC ACTIVITY: 2 superoxide + 2 H+ = O2 + H2O2. COFACTOR: Binds 1 copper ion per subunit. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. DISEASE: Defects in SOD1 are the cause of familial amyotrophic lateral sclerosis (FALS); also called amyotrophic lateral sclerosis 1 (ALS1 or ALS). ALS is a degenerative disorder of motorneurons in the cortex, brainstem and spinal cord. ALS is characterized by muscular weakness and atrophy beginning in the hands and spreading to the forearms and legs. Muscle fasciculations are commonly visible. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. ALS is sometimes referred to as Lou Gehrig disease after the famous American baseball player who was diagnosed with the disorder. FALS, the familial form of ALS, accounts for about 10% of the cases and is transmitted in an autosomal dominant manner. The mean age at onset of FALS is 45 years. MISCELLANEOUS: Zinc binding promotes dimerization. SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Glycerol (1:) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
A synthetic peptide (ASGEPVV LSGQIT) as part of mouse superoxide dismutase (SOD1) protein (aa: 24-36) conjugated to diphtheria toxoid
WB. A dilution of 1:500 to 1:1000 is recommended for this application. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Superoxide dismutase [Cu-Zn]; SOD1
Accession Number:
SODC_MOUSE
Reconstitution:
Reconstitute in 100µL of sterile water. Centrifuge to remove any insoluble material.
Specificity:
Specificity was confirmed by western blot detecting mouse superoxide dismutase (SOD1). | This antiserum is known to react with mouse and rat superoxide dismutase (SOD1) protein.
References:
Park KH et al. (2013) Postnatal muscle modification by myogenic factors modulates neuropathology and survival in an ALS mouse model. Nat Commun. 2013;4:2906.
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Rat, Other Mammals (Predicted)
Immunogen:
Purified bovine NF-H. Bovine intermediate filaments were prepared from spinal cords and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis.
Western Blotting (WB), Immunocytochemistry (ICC) and Immunocytochemistry (IHC). Suggested dilution for WB of 1:5,000-10,000. Suggested dilution for ICC/IHC is 1:1,000-1:5,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Accession Number:
P12036 NFH_HUMAN;
Reconstitution:
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody reacts with phosphorylated NF-H and is seen as a band of approx 200 kDa. | Rat. Predicted to react with other mammals due to sequence homology.
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Rat, Other Mammals (Predicted)
Immunogen:
Purified rat NF-H construct containing most of the tandem KSP repeats expressed and purified from E.coli.
Western Blotting (WB) and Immunocytochemistry (IC). Suggested dilution for WB of 1:5,000-10,000. Suggested dilution for IC is 1:500-1,000. This antibody stains dendritic and perikaryal neurofilaments particularly well. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845; NFH;
Accession Number:
P12036 NFH_HUMAN;
Reconstitution:
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
The specificity of this antibody has been confirmed by WB. This antibody reacts with noth phosphorylated NF-H at approx 200 kDa and non-phosphorylated NF-H at 160 kDa. | Rat. Predicted to react with other mammals due to sequence homology.
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:10,000 - 1:20,000 is recommended for WB. A dilution of 1:2,000 - 1:5,000 is recommended for ICC/IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Neurofilaments are composed of three intermediate filament proteins: light (~68 kDa), medium (~160 kDa) and heavy (~200 kDa), which are involved in the maintenance of the neuronal caliber. Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution of lyophilised antibody, aliquot and store at -20 °C for a higher stability. Avoid freeze-thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Hu, Rat, Ms, Fel, Chk
Immunogen:
A recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli.
Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A dilution of 1:1,000 - 1:5,000 is recommended for WB, ICC and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Neurofilament medium polypeptide; NF-M; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm;
Accession Number:
P12839 NFM_RAT;
Reconstitution:
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
Specifically recognizes the medium neurofilament subunit NF-L in WB. Band appears at ~145 kDa in WB from rodent and ~160 kDa for human and bovine WB. | Hu, Rat, Ms, Fel, Chk
Superoxide dismutase [Mn], mitochondrial destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems (Ref: SwissProt).
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized serum with 0.02% thimerosal
Storage Temp:
At least 12 months after purchase at 2 - 4 °C (lyophilized formulations).After reconstitution, aliquot and store at -20 °C for a higher stability.Avoid freeze-thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide (KHSLPDLPYDGALEPHINC) of human/rat/mouse mitochondrial manganese Superoxide Dismutase (SOD2), conjugated to Keyhole Limpet Hemocyanin.
IF, WB. Typical working dilutions for light microscopy are 1:500 to 1:1,000 depending on tissue and detection method. For IF, a dilution range of 1:50 to 1:100 is recommended. For WB, a dilution range of 1: 1,000 to 1: 4,000 is recommended. This antibody clearly detects a protein at approximately 24 kDa on WB of human brain tissue. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Superoxide dismutase [Mn], mitochondrial;
Accession Number:
P04179 SODM_HUMAN;
Reconstitution:
Reconstitute in sterile distilled water. Centrifuge to remove any insoluble material.
Specificity:
Mitochondrial manganese Superoxide Dismutase (SOD2), no cross reactivity to SOD1
SOD1 binds copper and zinc ions ans is one of two isozymes responsible for destroying free superoxide radicals which are normally produced within the cells and which are toxic to biological systems. SOD1 is a soluble cytoplasmic protein, acting as a homodimer to convert superoxide radicals to molecular oxygen and hydrogen peroxide. Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) which is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Host Animal:
Rabbit
Species Reactivity:
Human, Mouse (Negative), Rat (Negative)
Immunogen:
A peptide (HEKADDLGKGGNEESTKTG) corresponding to the amino acids 121-139 of human superoxide dismutase (SOD1) conjugated to diphtheria toxin has been used as the immunogen.
IHC, WB. This antibody works superbly in immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 1000 depending on tissue and detection method. For western blotting a dilution range of 1: 1000 to 1: 4000 is recommended. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Superoxide dismutase; SOD1;
Accession Number:
SODC_HUMAN
Reconstitution:
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
This antibody has been shown to be specific for superoxide dismutase (SOD1) protein. | Human. Does not detect mouse and rat SOD1.
SOD3 is a member of the superoxide dismutase protein family. SODs are antioxidant enzymes that catalyse the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. SOD3 is thought to protect the brain, lungs and other tissues from oxidative stress. It is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A small percentage of SOD3 is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the extracellular matrix.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 °C for a higher stability, and at 4 °C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.
Host Animal:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide (WTGEDSAEPNSDSAEWIRD) corresponding to the amino acids 18-37 of human extracellular superoxide dismutase conjugated to diphtheria toxin has been used as the immunogen.
IHC, WB. This antibody works superbly in Immunohistochemistry on frozen or paraffin embedded tissues. Antigen retrieval has been used in testing but may not be necessary. Typical working dilutions for routine immunohistochemistry are 1: 100 to 1: 2000 depending on tissue and detection method. For western blotting a dilution range of 1: 1000 to 1: 4000 is recommended. This antibody stains prominently specific human airway (probably Clara cells) and alveolar type II cells and some tubular cells in the kidney. Other tissues have not yet been tested but the gene expression has also been reported in heart, pancreas and placenta. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
EC-SOD; SOD3
Accession Number:
SODE_HUMAN
Reconstitution:
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
This antibody has been shown to be specific for extracellular superoxide dismutase protein. | Human
SOD1 binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals which are normally produced within the cells and which are toxic to biological systems. SOD1 is a soluble cytoplasmic protein, acting as a homodimer to convert superoxide radicals to molecular oxygen and hydrogen peroxide. Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) which is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis.
Western blotting (1:500 1:4000). Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
IHC, WB. A dilution of 1 µg/mL is recommended for both applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. DISEASE: Defects in GDNF may be a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. DISEASE: Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.
WB. Recommended to be used at a dilution of 1:1000 to 1:3000 for Western blot. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
CNTF is a survival promoting factor for different types of neurons in vitro and in vivo. The essential structural features for the biological function of human CNTF were investigated by Thier, M. et al. They showed that deletion of 14 N-terminal and 18 C-terminal amino acids significantly increased bioactivity compared to wild-type CNTF. FUNCTION: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Nervous system. PHARMACEUTICAL: CNTF is being tested under the name Axokine by Regeneron Pharmaceuticals for treatment of human motor neuron diseases, such as amyotrophic lateral sclerosis (ALS). As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. SIMILARITY: Belongs to the CNTF family.
Product Type:
Antibody
Antibody Type:
Polyclonal
Format:
Lyophilized
Storage Temp:
After reconstitution keep aliquots at -20 ºC for a higher stability, and at 4 ºC with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
IHC, WB, ELISA. A dilution of 1:500 to 4000 is recommended for these applications. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Alternative Names:
Ciliary neurotrophic factor
Accession Number:
CNTF_HUMAN
Reconstitution:
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Shelf Life:
12 months after purchase
Specificity:
This antibody specifically detects CNTF shown by western blot. | This antiserum to known to react with rat, mouse and human CNTF protein.
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