NEXTFLEX® UDI Barcoded Adapters (1-1,536) for Ultra High-Throughput Multiplexing
Sets of 384 UDI barcodes for multiplexing NGS libraries compatible with Illumina® Up to 1,536 (10-nt) adapters available for purchase off-the-shelf Every lot is functionally validated and tested for index purity by sequencing
Highest Sample Multiplexing Options for Illumina® Sequencing The superior design of the NEXTFLEX® Unique Dual Index barcodes allows a broad range of multiplexing users can multiplex anywhere between two samples and 1,536 samples in a single sequencing run. The wide range of multiplexing options enables barcode rotation (avoiding frequent use of the same barcode set) to mitigate run-to-run sample carryover and crosstalk. NEXTFLEX® Unique Dual Index barcodes are compatible with NEXTFLEX® library prep kits designed for Illumina® paired-end and single-read sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
Highest Level of Data Integrity The NEXTFLEX® Unique Dual Index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction. Each NEXTFLEX® Unique Dual Index barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices improves data integrity by correctly removing mis-assigned reads. Every lot is functionally validated and tested for index purity by sequencing.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix** (50 µM)
The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc).
* These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
** The Primer Mix 2.0 is only intended for use with the NEXTFLEX® Rapid XP DNA-Seq kit and the NEXTFLEX® Rapid DNA-Seq kit 2.0; if the primer mix is to be used with any other library kits of choice, additional dilutions may be required. For additional guidance, please contact us.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® UDI Barcoded Adapters (1-1,536) for Ultra High-Throughput Multiplexing
Sets of 384 UDI barcodes for multiplexing NGS libraries compatible with Illumina® Up to 1,536 (10-nt) adapters available for purchase off-the-shelf Every lot is functionally validated and tested for index purity by sequencing
Highest Sample Multiplexing Options for Illumina® Sequencing The superior design of the NEXTFLEX® Unique Dual Index barcodes allows a broad range of multiplexing users can multiplex anywhere between two samples and 1,536 samples in a single sequencing run. The wide range of multiplexing options enables barcode rotation (avoiding frequent use of the same barcode set) to mitigate run-to-run sample carryover and crosstalk. NEXTFLEX® Unique Dual Index barcodes are compatible with NEXTFLEX® library prep kits designed for Illumina® paired-end and single-read sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
Highest Level of Data Integrity The NEXTFLEX® Unique Dual Index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction. Each NEXTFLEX® Unique Dual Index barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices improves data integrity by correctly removing mis-assigned reads. Every lot is functionally validated and tested for index purity by sequencing.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix** (50 µM)
The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc).
* These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
** The Primer Mix 2.0 is only intended for use with the NEXTFLEX® Rapid XP DNA-Seq kit and the NEXTFLEX® Rapid DNA-Seq kit 2.0; if the primer mix is to be used with any other library kits of choice, additional dilutions may be required. For additional guidance, please contact us.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® UDI Barcoded Adapters (1-1,536) for Ultra High-Throughput Multiplexing
Sets of 384 UDI barcodes for multiplexing NGS libraries compatible with Illumina® Up to 1,536 (10-nt) adapters available for purchase off-the-shelf Every lot is functionally validated and tested for index purity by sequencing
Highest Sample Multiplexing Options for Illumina® Sequencing The superior design of the NEXTFLEX® Unique Dual Index barcodes allows a broad range of multiplexing users can multiplex anywhere between two samples and 1,536 samples in a single sequencing run. The wide range of multiplexing options enables barcode rotation (avoiding frequent use of the same barcode set) to mitigate run-to-run sample carryover and crosstalk. NEXTFLEX® Unique Dual Index barcodes are compatible with NEXTFLEX® library prep kits designed for Illumina® paired-end and single-read sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
Highest Level of Data Integrity The NEXTFLEX® Unique Dual Index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction. Each NEXTFLEX® Unique Dual Index barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices improves data integrity by correctly removing mis-assigned reads. Every lot is functionally validated and tested for index purity by sequencing.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix** (50 µM)
The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc).
* These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
** The Primer Mix 2.0 is only intended for use with the NEXTFLEX® Rapid XP DNA-Seq kit and the NEXTFLEX® Rapid DNA-Seq kit 2.0; if the primer mix is to be used with any other library kits of choice, additional dilutions may be required. For additional guidance, please contact us.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® UDI Barcoded Adapters (1-1,536) for Ultra High-Throughput Multiplexing
Sets of 384 UDI barcodes for multiplexing NGS libraries compatible with Illumina® Up to 1,536 (10-nt) adapters available for purchase off-the-shelf Every lot is functionally validated and tested for index purity by sequencing
Highest Sample Multiplexing Options for Illumina® Sequencing The superior design of the NEXTFLEX® Unique Dual Index barcodes allows a broad range of multiplexing users can multiplex anywhere between two samples and 1,536 samples in a single sequencing run. The wide range of multiplexing options enables barcode rotation (avoiding frequent use of the same barcode set) to mitigate run-to-run sample carryover and crosstalk. NEXTFLEX® Unique Dual Index barcodes are compatible with NEXTFLEX® library prep kits designed for Illumina® paired-end and single-read sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
Highest Level of Data Integrity The NEXTFLEX® Unique Dual Index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction. Each NEXTFLEX® Unique Dual Index barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices improves data integrity by correctly removing mis-assigned reads. Every lot is functionally validated and tested for index purity by sequencing.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix** (50 µM)
The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc).
* These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
** The Primer Mix 2.0 is only intended for use with the NEXTFLEX® Rapid XP DNA-Seq kit and the NEXTFLEX® Rapid DNA-Seq kit 2.0; if the primer mix is to be used with any other library kits of choice, additional dilutions may be required. For additional guidance, please contact us.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® UDI Barcoded Adapters (1-1,536) for Ultra High-Throughput Multiplexing
Sets of 384 UDI barcodes for multiplexing NGS libraries compatible with Illumina® Up to 1,536 (10-nt) adapters available for purchase off-the-shelf Every lot is functionally validated and tested for index purity by sequencing
Highest Sample Multiplexing Options for Illumina® Sequencing The superior design of the NEXTFLEX® Unique Dual Index barcodes allows a broad range of multiplexing users can multiplex anywhere between two samples and 1,536 samples in a single sequencing run. The wide range of multiplexing options enables barcode rotation (avoiding frequent use of the same barcode set) to mitigate run-to-run sample carryover and crosstalk. NEXTFLEX® Unique Dual Index barcodes are compatible with NEXTFLEX® library prep kits designed for Illumina® paired-end and single-read sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
Highest Level of Data Integrity The NEXTFLEX® Unique Dual Index barcodes utilize two unique indexes on each UDI adapter, with a hamming distance of at least three throughout the entire series to permit error correction. Each NEXTFLEX® Unique Dual Index barcode comprises the entire flow cell binding site, sequencing primer binding site and index sequences, thereby making PCR enrichment optional. Patterned flow cells, such as those found on the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms, have been known to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices improves data integrity by correctly removing mis-assigned reads. Every lot is functionally validated and tested for index purity by sequencing.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix** (50 µM)
The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc).
* These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
** The Primer Mix 2.0 is only intended for use with the NEXTFLEX® Rapid XP DNA-Seq kit and the NEXTFLEX® Rapid DNA-Seq kit 2.0; if the primer mix is to be used with any other library kits of choice, additional dilutions may be required. For additional guidance, please contact us.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® Unique Dual Index Barcodes 384 UDI adapters for mitigating sample misassignment due to index hopping
Unique dual index adapters for multiplexing up to 384 Illumina® sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Each lot of the NEXTFLEX® Unique Dual Index barcodes is functionally validated and tested for index purity by sequencing Compatible with both paired-end and single-read Illumina® sequencing
Data Security in Sequencing Applications
The NEXTFLEX® Unique Dual Index Barcodes are barcoded adapters, for sequencing on Illumina® platforms, that provide unprecedented data security in sequencing applications. Multiplexing with NEXTFLEX® Unique Dual Index barcodes drastically increases scale while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane. These barcoded adapters can be used with single read or paired-end read sequencing, and are compatible with NEXTFLEX® library prep kits designed for Illumina® sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
UDIs Prevent Data Ambiguity Caused by Index Hopping
The NEXTFLEX® Unique Dual Index Barcodes utilize two unique 8 nt indexes on each UDI adapter. The 8 nt indices are designed with a Hamming Distance of at least three throughout the entire series, permitting error correction. Each NEXTFLEX® Unique Dual Index Barcode comprises the entire flow cell binding site, sequencing primer binding site, and index sequences, making PCR enrichment optional. Patterned flow cells, such as those found on the NovaSeq® and HiSeq® 3000/4000 platforms have been recognized to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity.
Reduce Run-to-Run Sample Carryover & Sample Crosstalk with UDIs
Even users who dont routinely perform high-throughput multiplexing benefit from the superior design of the NEXTFLEX® Unique Dual Index Barcodes. The ability to avoid using the same set of indices consecutively reduces concerns about run-to-run sample carryover and sample crosstalk. They are designed to specifically address the index hopping, or spread of signal, phenomenon associated with Illumina® platforms that utilize a patterned flow cell.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix (12.5 µM)
* The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc) These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
For automation single-use plates, NEXTFLEX® Unique Dual Index Barcodes may be at a different specified concentration. Please inquire for additional details.
Background Info:
The NEXTflex unique dual index barcodes are barcoded adapters for sequencing on Illumina platforms that provide unprecedented data security in sequencing applications. Increased mis-assignment of indexes has been shown to occur on Illumina sequencing instruments that feature a patterned flow cell and exclusion amplification technology. The NEXTflex unique dual index barcodes are designed to specifically mitigate the index hopping or spread of signal phenomenon associated with Illumina platforms that utilise a patterned flow cell. Index mis-assignment can lead to increased false positive rates, which are especially detrimental to sensitive applications. Multiplexing with NEXTflex unique dual index barcodes drastically increases processing capacity while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane.
Illumina sequencing instruments that utilise a patterned flow cell and exclusion amplification technology is known to suffer from increased levels of sample mis-assignment. The use of NEXTflex unique dual index barcodes prevents such mis-assigned reads from appearing in final data sets allowing for the highest assurance of data integrity.
Rapid DNA, Cell Free DNA-Seq, Dual Index Barcodes, NEXTflex Unique Dual Index Barcoded Adapters 384 UDI Set
Additional Info:
Key Features:
Unique dual index adapters for multiplexing up to 96 Illumina sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Decreases level of mis-assigned reads in sequencing data Compatible with both paired-end and single-read Illumina sequencing
[These kits ship on dry ice.]
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® Unique Dual Index Barcodes 384 UDI adapters for mitigating sample misassignment due to index hopping
Unique dual index adapters for multiplexing up to 384 Illumina® sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Each lot of the NEXTFLEX® Unique Dual Index barcodes is functionally validated and tested for index purity by sequencing Compatible with both paired-end and single-read Illumina® sequencing
Data Security in Sequencing Applications
The NEXTFLEX® Unique Dual Index Barcodes are barcoded adapters, for sequencing on Illumina® platforms, that provide unprecedented data security in sequencing applications. Multiplexing with NEXTFLEX® Unique Dual Index barcodes drastically increases scale while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane. These barcoded adapters can be used with single read or paired-end read sequencing, and are compatible with NEXTFLEX® library prep kits designed for Illumina® sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
UDIs Prevent Data Ambiguity Caused by Index Hopping
The NEXTFLEX® Unique Dual Index Barcodes utilize two unique 8 nt indexes on each UDI adapter. The 8 nt indices are designed with a Hamming Distance of at least three throughout the entire series, permitting error correction. Each NEXTFLEX® Unique Dual Index Barcode comprises the entire flow cell binding site, sequencing primer binding site, and index sequences, making PCR enrichment optional. Patterned flow cells, such as those found on the NovaSeq® and HiSeq® 3000/4000 platforms have been recognized to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity.
Reduce Run-to-Run Sample Carryover & Sample Crosstalk with UDIs
Even users who dont routinely perform high-throughput multiplexing benefit from the superior design of the NEXTFLEX® Unique Dual Index Barcodes. The ability to avoid using the same set of indices consecutively reduces concerns about run-to-run sample carryover and sample crosstalk. They are designed to specifically address the index hopping, or spread of signal, phenomenon associated with Illumina® platforms that utilize a patterned flow cell.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix (12.5 µM)
* The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc) These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
For automation single-use plates, NEXTFLEX® Unique Dual Index Barcodes may be at a different specified concentration. Please inquire for additional details.
Background Info:
The NEXTflex unique dual index barcodes are barcoded adapters for sequencing on Illumina platforms that provide unprecedented data security in sequencing applications. Increased mis-assignment of indexes has been shown to occur on Illumina sequencing instruments that feature a patterned flow cell and exclusion amplification technology. The NEXTflex unique dual index barcodes are designed to specifically mitigate the index hopping or spread of signal phenomenon associated with Illumina platforms that utilise a patterned flow cell. Index mis-assignment can lead to increased false positive rates, which are especially detrimental to sensitive applications. Multiplexing with NEXTflex unique dual index barcodes drastically increases processing capacity while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane.
Illumina sequencing instruments that utilise a patterned flow cell and exclusion amplification technology is known to suffer from increased levels of sample mis-assignment. The use of NEXTflex unique dual index barcodes prevents such mis-assigned reads from appearing in final data sets allowing for the highest assurance of data integrity.
Rapid DNA, Cell Free DNA-Seq, Dual Index Barcodes, NEXTflex Unique Dual Index Barcoded Adapters 384 UDI Set
Additional Info:
Key Features:
Unique dual index adapters for multiplexing up to 96 Illumina sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Decreases level of mis-assigned reads in sequencing data Compatible with both paired-end and single-read Illumina sequencing
[These kits ship on dry ice.]
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® Unique Dual Index Barcodes 384 UDI adapters for mitigating sample misassignment due to index hopping
Unique dual index adapters for multiplexing up to 384 Illumina® sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Each lot of the NEXTFLEX® Unique Dual Index barcodes is functionally validated and tested for index purity by sequencing Compatible with both paired-end and single-read Illumina® sequencing
Data Security in Sequencing Applications
The NEXTFLEX® Unique Dual Index Barcodes are barcoded adapters, for sequencing on Illumina® platforms, that provide unprecedented data security in sequencing applications. Multiplexing with NEXTFLEX® Unique Dual Index barcodes drastically increases scale while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane. These barcoded adapters can be used with single read or paired-end read sequencing, and are compatible with NEXTFLEX® library prep kits designed for Illumina® sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
UDIs Prevent Data Ambiguity Caused by Index Hopping
The NEXTFLEX® Unique Dual Index Barcodes utilize two unique 8 nt indexes on each UDI adapter. The 8 nt indices are designed with a Hamming Distance of at least three throughout the entire series, permitting error correction. Each NEXTFLEX® Unique Dual Index Barcode comprises the entire flow cell binding site, sequencing primer binding site, and index sequences, making PCR enrichment optional. Patterned flow cells, such as those found on the NovaSeq® and HiSeq® 3000/4000 platforms have been recognized to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity.
Reduce Run-to-Run Sample Carryover & Sample Crosstalk with UDIs
Even users who dont routinely perform high-throughput multiplexing benefit from the superior design of the NEXTFLEX® Unique Dual Index Barcodes. The ability to avoid using the same set of indices consecutively reduces concerns about run-to-run sample carryover and sample crosstalk. They are designed to specifically address the index hopping, or spread of signal, phenomenon associated with Illumina® platforms that utilize a patterned flow cell.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix (12.5 µM)
* The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc) These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
For automation single-use plates, NEXTFLEX® Unique Dual Index Barcodes may be at a different specified concentration. Please inquire for additional details.
Background Info:
The NEXTflex unique dual index barcodes are barcoded adapters for sequencing on Illumina platforms that provide unprecedented data security in sequencing applications. Increased mis-assignment of indexes has been shown to occur on Illumina sequencing instruments that feature a patterned flow cell and exclusion amplification technology. The NEXTflex unique dual index barcodes are designed to specifically mitigate the index hopping or spread of signal phenomenon associated with Illumina platforms that utilise a patterned flow cell. Index mis-assignment can lead to increased false positive rates, which are especially detrimental to sensitive applications. Multiplexing with NEXTflex unique dual index barcodes drastically increases processing capacity while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane.
Illumina sequencing instruments that utilise a patterned flow cell and exclusion amplification technology is known to suffer from increased levels of sample mis-assignment. The use of NEXTflex unique dual index barcodes prevents such mis-assigned reads from appearing in final data sets allowing for the highest assurance of data integrity.
Rapid DNA, Cell Free DNA-Seq, Dual Index Barcodes, NEXTflex Unique Dual Index Barcoded Adapters 384 UDI Set
Additional Info:
Key Features:
Unique dual index adapters for multiplexing up to 96 Illumina sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Decreases level of mis-assigned reads in sequencing data Compatible with both paired-end and single-read Illumina sequencing
[These kits ship on dry ice.]
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
NEXTFLEX® Unique Dual Index Barcodes 384 UDI adapters for mitigating sample misassignment due to index hopping
Unique dual index adapters for multiplexing up to 384 Illumina® sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Each lot of the NEXTFLEX® Unique Dual Index barcodes is functionally validated and tested for index purity by sequencing Compatible with both paired-end and single-read Illumina® sequencing
Data Security in Sequencing Applications
The NEXTFLEX® Unique Dual Index Barcodes are barcoded adapters, for sequencing on Illumina® platforms, that provide unprecedented data security in sequencing applications. Multiplexing with NEXTFLEX® Unique Dual Index barcodes drastically increases scale while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane. These barcoded adapters can be used with single read or paired-end read sequencing, and are compatible with NEXTFLEX® library prep kits designed for Illumina® sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
UDIs Prevent Data Ambiguity Caused by Index Hopping
The NEXTFLEX® Unique Dual Index Barcodes utilize two unique 8 nt indexes on each UDI adapter. The 8 nt indices are designed with a Hamming Distance of at least three throughout the entire series, permitting error correction. Each NEXTFLEX® Unique Dual Index Barcode comprises the entire flow cell binding site, sequencing primer binding site, and index sequences, making PCR enrichment optional. Patterned flow cells, such as those found on the NovaSeq® and HiSeq® 3000/4000 platforms have been recognized to suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity.
Reduce Run-to-Run Sample Carryover & Sample Crosstalk with UDIs
Even users who dont routinely perform high-throughput multiplexing benefit from the superior design of the NEXTFLEX® Unique Dual Index Barcodes. The ability to avoid using the same set of indices consecutively reduces concerns about run-to-run sample carryover and sample crosstalk. They are designed to specifically address the index hopping, or spread of signal, phenomenon associated with Illumina® platforms that utilize a patterned flow cell.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (25 µM) NEXTFLEX® Primer Mix (12.5 µM)
* The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc) These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature.
For automation single-use plates, NEXTFLEX® Unique Dual Index Barcodes may be at a different specified concentration. Please inquire for additional details.
Background Info:
The NEXTflex unique dual index barcodes are barcoded adapters for sequencing on Illumina platforms that provide unprecedented data security in sequencing applications. Increased mis-assignment of indexes has been shown to occur on Illumina sequencing instruments that feature a patterned flow cell and exclusion amplification technology. The NEXTflex unique dual index barcodes are designed to specifically mitigate the index hopping or spread of signal phenomenon associated with Illumina platforms that utilise a patterned flow cell. Index mis-assignment can lead to increased false positive rates, which are especially detrimental to sensitive applications. Multiplexing with NEXTflex unique dual index barcodes drastically increases processing capacity while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane.
Illumina sequencing instruments that utilise a patterned flow cell and exclusion amplification technology is known to suffer from increased levels of sample mis-assignment. The use of NEXTflex unique dual index barcodes prevents such mis-assigned reads from appearing in final data sets allowing for the highest assurance of data integrity.
Rapid DNA, Cell Free DNA-Seq, Dual Index Barcodes, NEXTflex Unique Dual Index Barcoded Adapters 384 UDI Set
Additional Info:
Key Features:
Unique dual index adapters for multiplexing up to 96 Illumina sequencing libraries Mitigate index hopping or spread of signal that can occur during sequencing Decreases level of mis-assigned reads in sequencing data Compatible with both paired-end and single-read Illumina sequencing
[These kits ship on dry ice.]
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Cookies:
X
We use cookies to help personalise and improve your web experience.
By using our website you consent to our use of cookies, some of which may have already been set on your device.
View our Cookie Policy to learn more.
