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Product Search Results: AS07 217
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Product Search Results: AS07 217

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Agrisera AS07 217 50 µl £421.00
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Background Info: FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria as well as in chloroplasts. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules.
Product Type: Antibody
Antibody Type: Polyclonal
Format: Lyophilized
Storage Temp: Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal: Rabbit
Species Reactivity: Cylindrospermopsis raciborskii CS-505, Listeria monocytogenes (weak reaction), Synechococcus elongatus
Expected Species: Phaeodactylum tricornutum, Prochlorococcus sp.
Immunogen: Whole cyanobacterial (Anabaena PCC 7120) FtsZ protein, UniProt: Q3MC27 overexpressed in E.coli.
Applications: Immunofluorescence (IF), Immunolocalization (IL), Western blot (WB)
Data Sheet: PLEASE CLICK HERE TO DOWNLOAD
Additional Info: This antibody can be used as a loading control antibody in cyanobacteria. Immunofluorescence has been done by labelling Synechococcus elongatus cells at 30 C for 2 hours with FtsZ antibodies diluted to 1: 500 in blocking buffer. Detection images can be found in Kabeya et al (2010).This product can be sold containing ProClin if requested.
Application Details: 1 : 200 (IL) Immunogold-TEM, 1 : 500 (IF), 1 : 2000-1 : 5000 (WB)
Purity: Serum
Reconstitution: For reconstitution add 50 l of sterile water
Molecular Weight: 44,5 | 50 kDa
Not reactive in: higher plants
Selected references: Kurmayer et al. (2020). Chemically labeled toxins or bioactive peptides show a heterogeneous intracellular distribution and low spatial overlap with autofluorescence in bloom-forming cyanobacteria. Sci Rep. 2020 Feb 17;10(1):2781. doi: 10.1038/s41598-020-59381-w. (Immunofluorescence)Zhan et al. (2018). Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus. J Vis Exp. 2018 Nov 6;(141). doi: 10.3791/58603.MacCready et al. (2016). Robust Min-System Oscillation in the Presence of Internal Photosynthetic Membranes in Cyanobacteria. Molecular Microbiology November 5 2016. doi: 10.1111/mmi.13571Probst et al. (2014). Biology of a widespread uncultivated archaeon that contributes to carbon fixation in the subsurface. Nat Commun. 2014 Nov 26;5:5497. doi: 10.1038/ncomms6497.Miyagishima et al. (2014). DipM is required for peptidoglycan hydrolysis during chloroplast division. BMC Plant Biol. 2014 Mar 6;14(1):57. (immunofluorescence)Plominsky et al. (2013). Dinitrogen Fixation Is Restricted to the Terminal Heterocysts in the Invasive Cyanobacterium Cylindrospermopsis raciborskii CS-505. PLOS ONE, Open Access.Kabeya et al (2010). The YlmG protein has a conserved function related to the distribution of nucleoids in chloroplasts and cyanobacteria. BMC Plant Biology 10:57.
Special application note: To detect E.coli FtsZ protein we recommend a following product: AS10 715 | anti-FtsZ procaryotic cell division GTPase (bacterial), rabbit antibodyTo detect FtsZ protein in higher plants following antibodies are recommended: AS09 413 | Anti-FtsZ1 and 2 | Plant cell division protein FtsZ1 and FtsZ2, rabbit antibodiesAS13 2651 | Anti-FtsZ2 | Plant cell division protein ftsZ2, rabbit antibodies
UniProt number: Q3MC27
Research area: CyanoBacterial antibodies antibodies