| Background Info: |
Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN1 is highly abundant in the vascular elements of roots nodules, flowers and fruits, functioning in the assimilation of ammonium and the biosynthesis of glutamine for nitrogen transport. GLN2 is encoded by a single gene and is highly abundant in leaf mesophyll chloroplasts. Here GLN functions in the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts |
| Product Type: |
Antibody |
| Antibody Type: |
Polyclonal |
| Format: |
Lyophilized |
| Storage Temp: |
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Host Animal: |
Rabbit |
| Species Reactivity: |
Arabidopsis thaliana, Eragrostis tef, Gracilaria gracilis (red algae), Gracilaria lemaneiformis, Leptodictyum riparium (Hedw.) Warnst (moss), Medicago truncatula, Physcomitrium patens, Pinus strobus, Spinacia oleracea, Solanum lycopersicum, Triticum aestivum, Zea mays |
| Expected Species: |
Brachypodium distachyon, Brassica napus, Camellia sinensis, Citrus clementina, Cucumis melo, Daphnia magna, Datisca glomerata, Emiliania huxleyi, Eucalyptus grandis, Gazania splendens, Genlisea aurea, Glycine max, Helianthus annuus, Hordeum vulgare, Oryza sativa, Panax quinquefolius, Phaseolus angularis, Phytophthora cinnamomi, Populus trichocarpa, Saccharum officinarum, Securigera parviflora, Solanum lycopersicum, Solanum tuberosum, Stevia rebaudiana, Theobroma cacao, Zea mays, Vitis labruscaGLN1 dicots including: Brassica napus, Phaseolus vulgaris, monocots including: Hordeum vulgare, Oryza sativa, trees: Pinus sylvestris, Populus sp., Zosteria marinaGLN2 dicots including: Brassica napus, Glycine max, Phaseolus vulgaris, monocots including: Triticum aestivum, Oryza sativaGLN3: Zea mays,GLN1 in algae: Chlamydomonas reinhardiiSpecies of your interest not listed? Contact us |
| Immunogen: |
KLH-conjugated synthetic peptide derived from a wide range of available sequences including all isoforms of Arabidopsis thaliana GLN1-1,1-2,1-3 and 1-4, (At5g37600, At1g66200, At3g17820, At5g16570) |
| Applications: |
Western blot (WB) |
| Data Sheet: |
PLEASE CLICK HERE TO DOWNLOAD |
| Application Details: |
1 : 10 000 (WB) |
| Purity: |
Serum |
| Reconstitution: |
For reconstitution add 50 l of sterile water |
| Molecular Weight: |
39-40 kDa (GLN1,cytoplasmic form) , 44-45 kDa (GLN2, chloroplastic form) |
| Not reactive in: |
No confirmed exceptions from predicted reactivity are currently known |
| Selected references: |
Maresca et al. (2021) Biological responses to heavy metal stress in the moss Leptodictyum riparium (Hedw.) Warnst. Ecotoxicol Environ Saf. 2022 Jan 1;229:113078. doi: 10.1016/j.ecoenv.2021.113078. Epub 2021 Dec 17. PMID: 34929502.Silva et al. (2019). Characterization of plant glutamine synthetase S-nitrosation. Nitric Oxide. 2019 Apr 23;88:73-86. doi: 10.1016/j.niox.2019.04.006.Wang et al. (2018). Response of Gracilaria lemaneiformis to nitrogen deprivation. Algal Research Volume 34, September 2018, Pages 82-96.Witzel et al. (2017). Temporal impact of the vascular wilt pathogen Verticillium dahliae on tomato root proteome. J Proteomics. 2017 Oct 3;169:215-224. doi: 10.1016/j.jprot.2017.04.008.Silva et al. (2015). Possible role of glutamine synthetase of the prokaryotic type (GSI-like) in nitrogen signaling in Medicago truncatula. Volume 240, November 2015, Pages 98–108. |
| Special application note: |
The antibody will recognize both, cytoplasmic and chloroplastic forms of the GS enzyme |
| UniProt number: |
Q56WN1 , Q8LCE1 , Q9LVI8 , Q9FMD9 |
| TAIR number: |
AT5G37600 |
| Research area: |
Global Antibodies |
| Code: |
Stress20 |
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