Background Info: |
Tubulin beta (TUB) together with alpha tubulin is making up microtubules. |
Product Type: |
Antibody |
Antibody Type: |
Polyclonal |
Format: |
Lyophilized |
Storage Temp: |
Store lyophilized/reconstituted at -20 °C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
Host Animal: |
Rabbit |
Species Reactivity: |
Arabidopsis thaliana (including suspension cells), Chlamydomonas reinhardii, Medicago sativa |
Expected Species: |
Brassica napus, Glycine max, Hordeum vulgare, Micromonoas pusilla, Nicotiana attenuata, Oryza sativa, Ostreococcus lucimarinus, Picea sitcHensis, Pisum sativum, Physcomitrium patens, Populus trichocarpa, Saccharomyces cerevisiae, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Zea mays, Vigna radiata, Vitis viniferaSpecies of your interest not listed? Contact us |
Immunogen: |
KLH-conjugated peptide derived from available tubulin beta chain sequences (located at a surface loop) including all Arabidopsis thaliana tubulin beta-2/beta-3 chain P29512, beta-4 chain P24636(At5g44340), beta-5 chain P29513(At1g20010), beta-6 chain P29514(At5g12250), beta-7 chain P29515(At2g29550), beta-8 chain P29516(At5g23860), beta-9 chain P29517(At4g20890). |
Applications: |
immunofluorescence (IF), Western blot (WB) |
Data Sheet: |
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Additional Info: |
Metal induced stress affected the expression of tubulin, and that therefore, this protein cannot be used as a loading control under that type of conditions. More information can be found here. |
Application Details: |
1 : 1000 (IF), 1 : 500 (WB) |
Purity: |
Immunogen affinity purified serum in PBS pH 7.4. |
Reconstitution: |
For reconstitution add 100 µl of sterile water |
Molecular Weight: |
49 | 49 kDa (Arabidopsis thaliana) |
Not reactive in: |
No confirmed exceptions from predicted reactivity are currently known |
Selected references: |
Durian et al. (2019). PROTEIN PHOSPHATASE 2A-B' gamma controls Botrytis cinerea resistance and developmental leaf senescence. Plant Physiol. 2019 Oct 28. pii: pp.00893.2019. doi: 10.1104/pp.19.00893.Wang et al. (2017). The inhibition of protein translation mediated by AtGCN1 is essential for cold tolerance in Arabidopsis thaliana. Plant Cell Environ. 2017 Jan;40(1):56-68. doi: 10.1111/pce.12826.Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113 |
Special application note: |
Please, note that tubulin beta is only detected in actively dividing meristematic cells (see immunolocalization image below). Therefore to allow detection on a western blot, analyzed material must contain enough meristematic cells with dividing activity.Signal in a western blot application in Chlamydomonas reinhardtii is obtained with a load of 100 g/well and a dilution of 1: 500. |
UniProt number: |
P29512 , P24636 , P29513 , P29514 , P29515 , P29516 , P29517 |
TAIR number: |
AT5G62690 |
Research area: |
Arabidopsis thaliana antibodies |