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Product Search Results: AS15 2871
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Product Search Results: AS15 2871

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Product - please click for more details Brand Cat No Size Price (£)

Agrisera AS15 2871 50 µg £272.00
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Background Info: FLAG-tag is a tag that can be added to a protein sequence motif DYKXXD. It can be used for affinity chromatography, to separate recombinant protein from wt proteins. It can also be used to isolate protein complexes with multiple subunits.
Product Type: Antibody
Antibody Type: Monoclonal
Format: Liquid
Storage Temp: Store at -20 °C for up to 2 years.
Host Animal: Mouse
Species Reactivity: N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins
Immunogen: KLH-conjugated DYKDDDDK (FLAG) synthetic peptide
Applications: Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
Data Sheet: PLEASE CLICK HERE TO DOWNLOAD
Application Details: 1 : 1000-5000 (1-5ng) (WB) (incubate for one hour at room temperature), 1 : 500-1 :2000 (IS) For best results with other assays (Dot, ELISA, IP), please determine optimal working dilution by end user
Conjugation: IgG1
Isotype: IgG1
Purity: Total IgG. Protein A purified in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Special application note: Working dilution for Dot Blot, ELISA and IP needs to be determined experimentally.Antibody is purified on Protein A.
Research area: DYKDDDDK (binds to SigmaFLAG )