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Product Search Results: AS15 2987
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Product Search Results: AS15 2987

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Agrisera AS15 2987 100 µg at 1 mg/ml £526.00
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Background Info: GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Product Type: Antibody
Antibody Type: Polyclonal
Format: Liquid
Storage Temp: Store at -20 °C. Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Host Animal: Rabbit
Species Reactivity: Native GFP, Recombinant GFP (E,coli), all variants of GFP, including EGFP
Immunogen: Highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212
Applications: ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
Data Sheet: PLEASE CLICK HERE TO DOWNLOAD
Additional Info: Minimal cross-reactivity with E.coli proteins.Antibody reactivity to mVenus or EBFP2 has not yet been determined.
Application Details: 1 : 5000-1 : 25 000 (ELISA), 1: 100 - 1 : 500 (IF), 1 : 2000-1 : 10 000 (WB)
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Selected references: Sun et al. (2021) The epigenetic factor FVE orchestrates cytoplasmic SGS3-DRB4-DCL4 activities to promote transgene silencing in Arabidopsis. Sci Adv. 2021 Aug 4;7(32):eabf3898. doi: 10.1126/sciadv.abf3898. PMID: 34348894; PMCID: PMC8336953.Stelate et al. (2021) Correlative Light-Environmental Scanning Electron Microscopy of Plasma Membrane Efflux Carriers of Plant Hormone Auxin. Biomolecules. 2021 Sep 26;11(10):1407. doi: 10.3390/biom11101407. PMID: 34680040; PMCID: PMC8533460.Wieczorek et al. (2020) Development of a New Tomato Torrado Virus-Based Vector Tagged with GFP for Monitoring Virus Movement in Plants. Viruses. 2020 Oct 20;12(10):1195. doi: 10.3390/v12101195. PMID: 33092281; PMCID: PMC7588970.Kulich et al. (2018). Deubiquitinase OTU5 affects Root Responses to Phosphate Starvation. Plant Physiol. 2018 Jan 4. pii: pp.01693.2017. doi: 10.1104/pp.17.01693.
Special application note: 10 mM TRIS buffer pH 8,0 containing 0,02% sodium azide
UniProt number: P42212
Research area: GFP/EGFP/BFP/CFP/YFP/RFP