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Product Search Results: AS19 4295

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Product Search Results: AS19 4295

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Agrisera AS19 4295 50 µl £284.00
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Background Info: South et. al (2019). Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field. Science 2019 Jan 4;363(6422), DOI: 10.1126/science.aat9077
Product Type: Antibody
Antibody Type: Polyclonal
Format: Lyophilized
Host Animal: Rabbit
Species Reactivity: Nicotiana tabacum
Expected Species: Arabidopsis thaliana, Cajanus cajan, Cinnamomum micranthum f. kanehirae, Cucumis sativus, Cucurbita maxima, Fagus sylvatica, Glycine max, Jatropha curcas, Morus notabilis, Mucuna pruriens, Parasponia andersonii, Theobroma cacao, Trema orientale
Immunogen: KLH-conjugated peptide derived from Cucurbita maxima UniProt: P24571
Applications: Western blot (WB)
Additional Info: Experimental contitions: 5 µg of total protein extracted freshly from 3-4 weeks old plant leaves with a blender at 4 °C in 300 mM Sorbitol, 50 mM HEPES, 5mM MgCl2. Separated on 10 % SDS-PAGE and blotted 1h to PVDF, semi-dry. Blot was blocked with 6 % milk for 1h 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 ON at 4°C with agitation. According to South et. al (2019).
Application Details: 1 : 1000 (WB)
Purity: Serum
Reconstitution: For reconstitution add 50 µl, of sterile water.
Related products: Collection of antibodies for carbohydrate metabolism
Molecular Weight: 65 kDa
Not reactive in: Sorghum bicolor
Special application note: Malate synthase is involved in the synthesis of (S)-malate from isocitrate, where it catalyses the reaction: acetyl-CoA + glyoxylate + H(2)O = (S)-malate + CoA + H(+). This is part of the glyoxylate cycle, which is itself part of carbohydrate metabolism.
UniProt number: P24571
TAIR number: TAIR