Newmarket Scientific
Newmarket Scientific

Product Search Results: AS19 4297

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Product Search Results: AS19 4297

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Agrisera AS19 4297 50 µl £284.00
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Background Info: Huokko et al. (2019). Thylakoid Localized Type 2 NAD(P)H Dehydrogenase NdbA Optimizes Light-Activated Heterotrophic Growth of Synechocystis sp. PCC 6803. Plant Cell Physiol. 2019 Mar 7. pii: pcz044. doi: 10.1093/pcp/pcz044.
Product Type: Antibody
Antibody Type: Polyclonal
Format: Lyophilized
Storage Temp: 18 month shelf life at 2°C to 8°C.Mixed working reagent is stable for several days at room temperature or at 4°C.Exceptional lot to lot consistency.Superior stability.
Host Animal: Rabbit
Species Reactivity: Synechocystis sp. PCC 68
Expected Species: Bacillus subtilis
Immunogen: KLH-conjugated peptide derived from NdbA protein sequence of Synechocystis sp. PCC 6803, UniProt:P73739 Chosen peptide is not conserved in NdbB and NdbC.
Applications: Western blot (WB)
Additional Info: Protein extraction: harvested cells were suspended in a buffer containing 50 mM Hepes-NaOH, pH 7.5, 30 mM CaCl2, 800 mM sorbitol, 1 mM \x{03b5}-amino-n-caproic acid, and the cells were broken by vortexing 6×1 min at 4°C in the presence of glass beads. Protein samples were solubilized in Laemmli buffer containing 6 M urea and separated on a gel in presence of 6M urea.
Application Details: 1 : 2000 (WB)
Purity: Serum
Reconstitution: For reconstitution add 50 µl, of sterile water.
Related products: collection of antibodies to cyanobacterial proteins
Molecular Weight: 49 | 46 kDa
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Special application note: NdbA (Thylakoid Localized Type 2 NAD(P)H Dehydrogenase) is localized to the thylakoid membrane and expressed at a very low level in the widl type under photoautotrophic growth. NdbA is induced substantially under light-activated heterotophic growth conditions.
UniProt number: UniProt