MBD2 and MDB3 are non-enzymatic subunits of the NuRD (nucleosome remodelling and deacetylation) complex. MBD2 and MBD3 are close relatives and probably descend via gene duplication from an ancestral MBD2/MBD3, that is present in some metazoans as for instance Caenorhabditis elegans and Drosophila. Outside the MBD domain, MBD2 and MBD3 share almost 80% homology; they both have an MBD and a coiled-coil domain (CC). Apart from this common domain, MBD2 contains an additional N-terminal glycine-arginine (GR) rich domain and a transcriptional repressor domain (TRD), whereas MBD3 has a C-terminal poly-glutamate region. Three isoforms have been described for MBD2 protein: the full length MBD2a, MBD2b lacking the N-terminal GR repeat and MBD2c that is a testis specific isoform lacking the C-terminus. Also MBD3 presents three isoforms: Mbd3b the major isoform in embryonic stem cells, Mbd3a and a smaller isoform Mbd3c. The crucial difference between MBD2 and MBD3 is that MBD3 does not bind methylated DNA, because it lacks four conserved amino acids in the MBD domain. (Menafra R, Stunnenberg HG. MBD2 and MBD3: elusive functions and mechanisms. Frontiers in Genetics. 2014;5:428. doi:10.3389/fgene.2014.00428.)
Product Type:
NS Reagents Antibody
Antibody Type:
Monoclonal
Format:
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide.
MBD2 and MDB3 are non-enzymatic subunits of the NuRD (nucleosome remodelling and deacetylation) complex. MBD2 and MBD3 are close relatives and probably descend via gene duplication from an ancestral MBD2/MBD3, that is present in some metazoans as for instance Caenorhabditis elegans and Drosophila. Outside the MBD domain, MBD2 and MBD3 share almost 80% homology; they both have an MBD and a coiled-coil domain (CC). Apart from this common domain, MBD2 contains an additional N-terminal glycine-arginine (GR) rich domain and a transcriptional repressor domain (TRD), whereas MBD3 has a C-terminal poly-glutamate region. Three isoforms have been described for MBD2 protein: the full length MBD2a, MBD2b lacking the N-terminal GR repeat and MBD2c that is a testis specific isoform lacking the C-terminus. Also MBD3 presents three isoforms: Mbd3b the major isoform in embryonic stem cells, Mbd3a and a smaller isoform Mbd3c. The crucial difference between MBD2 and MBD3 is that MBD3 does not bind methylated DNA, because it lacks four conserved amino acids in the MBD domain. (Menafra R, Stunnenberg HG. MBD2 and MBD3: elusive functions and mechanisms. Frontiers in Genetics. 2014;5:428. doi:10.3389/fgene.2014.00428.)
Product Type:
NS Reagents Antibody
Antibody Type:
Monoclonal
Format:
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide.
Methyl-CpG-Binding Domain Protein 1 (MBD1) is a member of the MBD transcriptional repressor family and is predominantly expressed in neurons. It binds to methylated sequences in DNA, influencing transcription. It has been shown to play a role in chromatin modification through interaction with the histone H3K9 methyltransferase SETDB1. H3K9me3 is a repressive modification.
MBD1 acts as an epigenetic regulator via different mechanisms, such as the formation of the MCAF1/MBD1/SETDB1 complex or the MBD1-HDAC3 complex.
Product Type:
NS Reagents Antibody
Antibody Type:
Monoclonal
Format:
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide.
Methyl-CpG-Binding Domain Protein 1 (MBD1) is a member of the MBD transcriptional repressor family and is predominantly expressed in neurons. It binds to methylated sequences in DNA, influencing transcription. It has been shown to play a role in chromatin modification through interaction with the histone H3K9 methyltransferase SETDB1. H3K9me3 is a repressive modification.
MBD1 acts as an epigenetic regulator via different mechanisms, such as the formation of the MCAF1/MBD1/SETDB1 complex or the MBD1-HDAC3 complex.
Product Type:
NS Reagents Antibody
Antibody Type:
Monoclonal
Format:
100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide.
Transcription and Translation, Nucleic Acid Modification, Protein Modification, Transcription, Chromatin
NS Product Area:
Protein and Nucleic Acid Modification
Purification:
Protein G Purified
Release Date:
November 2017
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