The Biosensis NGFR/p75 enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of human p75 neurotrophin receptor in less than 4 hours in urine, serum, citrate-plasma, CSF and cell culture supernatants only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.Note that capture and detection antibodies have been optimized for increased assay sensitivity, and reagents from previous lots should not be used. This optimized human p75 ECD ELISA assay demonstrates an increased sensitivity, lowering the LOD about 8-fold to the previous assay version. It represents the most sensitive NGFR/p75 ECD ELISA assay available today with a detection limit of at least 6 pg/mL, and a quantification range of 7.8 - 500 pg/mL.This ELISA kit consists of a pre-coated mouse monoclonal anti-p75 ECD capture antibody, a biotinylated mouse monoclonal anti-p75 ECD detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75 ECD present in samples and protein standards. A human p75 ECD positive control (QC sample) is provided to assure consistent assay performance.This NGFR/p75 ECD ELISA kit employs an in-house recombinant human p75 ECD-Fc chimera as standard that has been validated against an externally available p75 ECD Fc protein. While there is a current lack of a commercially available, true human p75 ECD standard, this ELISA kit allows quantification of human p75 ECD as p75 ECD-Fc human equivalents. Please note that the antibodies used in this ELISA do not cross-react with mouse p75 ECD. Note: For research use only. Not for diagnostic and clinical purposes.
Background Info:
The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C.
Species Reactivity:
This ELISA kit does not cross-react with mouse p75 ECD protein.
The Biosensis NGFR/p75 ECD enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of human p75 neurotrophin receptor in less than 4 hours in urine, serum, citrate-plasma, CSF and cell culture supernatants only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.Note that capture and detection antibodies have been optimized for increased assay sensitivity, and reagents from previous lots should not be used. This optimized human p75 ECD ELISA assay demonstrates an increased sensitivity, lowering the LOD about 8-fold to the previous assay version. It represents the most sensitive NGFR/p75 ECD ELISA assay available today, with a detection limit of at least 6 pg/mL, and a quantification range of 7.8 - 500 pg/mL.This ELISA kit consists of a pre-coated mouse monoclonal anti-p75 ECD capture antibody, a biotinylated mouse monoclonal anti-p75 ECD detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75 ECD present in samples and protein standards. A human p75 ECD positive control (QC sample) is provided to assure consistent assay performance.This NGFR/p75 ECD ELISA kit employs an in-house recombinant human p75 ECD -Fc chimera as standard that has been validated against an externally available p75 ECD -Fc protein. While there is a current lack of a commercially available, true human p75 ECD standard, this ELISA kit allows quantification of human p75 ECD as p75 ECD -Fc human equivalents. Please note that the antibodies used in this ELISA do not cross-react with mouse p75 ECD. Note: For research use only. Not for diagnostic and clinical purposes.
Background Info:
The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C.
Species Reactivity:
This ELISA kit does not cross-react with mouse p75 ECD protein.
The Biosensis NGFR/p75 ECD enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mouse p75 ECD in less than 4 hours n cell culture supernatants and urine only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.This ELISA kit consists of a pre-coated mouse monoclonal anti-p75 ECD capture antibody, a goat anti- p75 ECD detection antibody and a horseradish peroxidase (HRP)-conjugated anti-goat antibody. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75 ECD present in samples and protein standards.This NGFR/p75 ECD ELISA kit employs a recombinant mouse p75 ECD -Fc chimera protein as standard. While there is a current lack of a true mouse p75 ECD standard, this ELISA kit allows quantification of mouse p75 ECD as p75 ECD -Fc mouse equivalents. Please note that the antibodies used in this ELISA cross-react with human NGFR/p75 ECD. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C.
Species Reactivity:
The antibodies used in this ELISA kit are known to cross-react with human p75ECD protein.
The ELISA kit box contains 1 x 96-well pre-coated strip plate, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Shelf Life:
12 months from purchase.
Specificity:
Mouse. | The antibodies used in this ELISA kit are known to cross-react with human p75ECD protein.
Assay Range:
62.5 - 4000 pg/mL.
Sensitivity:
This ELISA kit typically detects 20-40 pg/mL of mouse p75ECD (defined as blank OD plus 3x the standard deviation of the blank OD, n=10).
References:
Maejima H, Kanemura N, Kokubun T, Murata K, Takayanagi K (2017) Exercise enhances cognitive function and neurotrophin expression in the hippocampus accompanied by changes in epigenetic programming in senescence-accelerated mice. Neurosci Lett. doi: 10.1016/j.neulet.2017.11.023. [Epub ahead of print]. Application: Mouse hippocampus homogenates.
The Biosensis NGFR/p75 ECD enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mouse p75 ECD in less than 4 hours n cell culture supernatants and urine only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.This ELISA kit consists of a pre-coated mouse monoclonal anti-p75 ECD capture antibody, a goat anti- p75 ECD detection antibody and a horseradish peroxidase (HRP)-conjugated anti-goat antibody. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75ECD present in samples and protein standards.This NGFR/p75 ECD ELISA kit employs a recombinant mouse p75 ECD -Fc chimera protein as standard. While there is a current lack of a true mouse p75 ECD standard, this ELISA kit allows quantification of mouse p75 ECD as p75 ECD -Fc mouse equivalents. Please note that the antibodies used in this ELISA cross-react with human NGFR/p75 ECD . This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Background Info:
The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.
Product Type:
ELISA
Format:
Ready to use Assay, complete with pre-coated plates, Concentrated Buffers, Detection reagents and complete instructions.
Storage Temp:
Store at 2-8 °C.
Species Reactivity:
The antibodies used in this ELISA kit are known to cross-react with human p75ECD protein.
The ELISA kit box contains 2 x 96-well pre-coated strip plates, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
Shelf Life:
12 months from purchase.
Specificity:
Mouse. | The antibodies used in this ELISA kit are known to cross-react with human p75ECD protein.
Assay Range:
62.5 - 4000 pg/mL
Sensitivity:
This ELISA kit typically detects 20-40 pg/mL of mouse p75ECD (defined as blank OD plus 3x the standard deviation of the blank OD, n=10).
References:
Maejima H, Kanemura N, Kokubun T, Murata K, Takayanagi K (2017) Exercise enhances cognitive function and neurotrophin expression in the hippocampus accompanied by changes in epigenetic programming in senescence-accelerated mice. Neurosci Lett. doi: 10.1016/j.neulet.2017.11.023. [Epub ahead of print]. Application: Mouse hippocampus homogenates.
Related Products:
ELISA
Trademark:
Biosensis Rapid TM kits
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