Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples intended for use to produce libraries for NGS RNA-Seq experiments. Removal of rRNA avoids the waste of reagents and bioinformatic resources to sequence and align the ~85% of total RNA comprised of rRNA, which is generally not a target of interest in NGS sequencing experiments.
The NEXTFLEX® Poly(A) Beads 2.0 offer improved mRNA yields, lower rRNA contamination, and a more simplified protocol, verified from 10 ng - 5 µg of total RNA for a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep.
Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. These beads have been validated with the new and improved NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0.
The NEXTFLEX® Poly(A) beads 2.0 are an easy and cost-effective method for removing rRNA in RNA-Seq libraries. This product takes advantage of the tract of adenosine residues present at the 3 ends of the vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines (oligo dT) immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.
The NEXTFLEX® Poly(A) Beads 2.0 contains enough material to extract mRNA from 8, 48 or 96 total RNA samples for subsequent RNA-seq library prep or other applications. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at 4°C.
These beads have been validated with the following kits: NEXTflex Directional RNA-Seq Kit NEXTflex Directional RNA-Seq Kit 2.0
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples intended for use to produce libraries for NGS RNA-Seq experiments. Removal of rRNA avoids the waste of reagents and bioinformatic resources to sequence and align the ~85% of total RNA comprised of rRNA, which is generally not a target of interest in NGS sequencing experiments.
The NEXTFLEX® Poly(A) Beads 2.0 offer improved mRNA yields, lower rRNA contamination, and a more simplified protocol, verified from 10 ng - 5 µg of total RNA for a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep.
Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. These beads have been validated with the new and improved NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0.
The NEXTFLEX® Poly(A) beads 2.0 are an easy and cost-effective method for removing rRNA in RNA-Seq libraries. This product takes advantage of the tract of adenosine residues present at the 3 ends of the vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines (oligo dT) immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.
The NEXTFLEX® Poly(A) Beads 2.0 contains enough material to extract mRNA from 8, 48 or 96 total RNA samples for subsequent RNA-seq library prep or other applications. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at 4°C.
These beads have been validated with the following kits: NEXTflex Directional RNA-Seq Kit NEXTflex Directional RNA-Seq Kit 2.0
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples intended for use to produce libraries for NGS RNA-Seq experiments. Removal of rRNA avoids the waste of reagents and bioinformatic resources to sequence and align the ~85% of total RNA comprised of rRNA, which is generally not a target of interest in NGS sequencing experiments.
The NEXTFLEX® Poly(A) Beads 2.0 offer improved mRNA yields, lower rRNA contamination, and a more simplified protocol, verified from 10 ng - 5 µg of total RNA for a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep.
Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. These beads have been validated with the new and improved NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0.
The NEXTFLEX® Poly(A) beads 2.0 are an easy and cost-effective method for removing rRNA in RNA-Seq libraries. This product takes advantage of the tract of adenosine residues present at the 3 ends of the vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines (oligo dT) immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.
The NEXTFLEX® Poly(A) Beads 2.0 contains enough material to extract mRNA from 8, 48 or 96 total RNA samples for subsequent RNA-seq library prep or other applications. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at 4°C.
These beads have been validated with the following kits: NEXTflex Directional RNA-Seq Kit NEXTflex Directional RNA-Seq Kit 2.0
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
The NEXTflex Rapid Directional RNA-Seq Kit contains enough material to prepare 8 or 48 RNA samples for Illumina compatible sequencing. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Even Coverage Uniformity, Low Duplication Rates, >99% Strand Specificity
The NEXTFLEX® rapid directional RNA-seq kit 2.0 produces libraries for Illumina® sequencing with even coverage uniformity, low duplication rates, >99% strand specificity and minimal rRNA contamination when used with the NEXTFLEX® Poly(A) Beads 2.0. This kit includes reverse transcriptase, necessary library preparation reagents, and cleanup/size selection beads optimized to ensure reliable performance. The kit involves a simple library preparation protocol that has been validated with the updated NEXTFLEX® poly(A) beads 2.0 to accommodate total RNA as input. The NEXTFLEX® rapid directional RNA-seq kit 2.0 is designed to be used with NEXTFLEX® barcodes, which will be provided to early access users. All NEXTFLEX® barcodes are color-balanced and have undergone proprietary purity QC metrics to give you reliable sequencing results for every sample.
BENEFITS:- >> Reliable: Even coverage uniformity with low duplication rate >> Convenient: Optimized with reverse transcriptase and cleanup/size selection beads >> Streamlined: Simple protocol validated with NEXTFLEX® poly(A) beads 2.0 >> Flexible: Designed to work with NEXTFLEX® color-balanced barcodes that allow a wide range of multiplexing (up to 384 samples in one run) >> Efficient: Automated on the Sciclone® G3 NGSx and Zephyr® G3 NGS workstations
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5138-07
The NEXTflex Rapid Directional RNA-Seq Kit contains enough material to prepare 8 or 48 RNA samples for Illumina compatible sequencing. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Even Coverage Uniformity, Low Duplication Rates, >99% Strand Specificity
The NEXTFLEX® rapid directional RNA-seq kit 2.0 produces libraries for Illumina® sequencing with even coverage uniformity, low duplication rates, >99% strand specificity and minimal rRNA contamination when used with the NEXTFLEX® Poly(A) Beads 2.0. This kit includes reverse transcriptase, necessary library preparation reagents, and cleanup/size selection beads optimized to ensure reliable performance. The kit involves a simple library preparation protocol that has been validated with the updated NEXTFLEX® poly(A) beads 2.0 to accommodate total RNA as input. The NEXTFLEX® rapid directional RNA-seq kit 2.0 is designed to be used with NEXTFLEX® barcodes, which will be provided to early access users. All NEXTFLEX® barcodes are color-balanced and have undergone proprietary purity QC metrics to give you reliable sequencing results for every sample.
BENEFITS:- >> Reliable: Even coverage uniformity with low duplication rate >> Convenient: Optimized with reverse transcriptase and cleanup/size selection beads >> Streamlined: Simple protocol validated with NEXTFLEX® poly(A) beads 2.0 >> Flexible: Designed to work with NEXTFLEX® color-balanced barcodes that allow a wide range of multiplexing (up to 384 samples in one run) >> Efficient: Automated on the Sciclone® G3 NGSx and Zephyr® G3 NGS workstations
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5138-08
The NEXTflex Rapid Directional RNA-Seq Kit contains enough material to prepare 8 or 48 RNA samples for Illumina compatible sequencing. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Even Coverage Uniformity, Low Duplication Rates, >99% Strand Specificity
The NEXTFLEX® rapid directional RNA-seq kit 2.0 produces libraries for Illumina® sequencing with even coverage uniformity, low duplication rates, >99% strand specificity and minimal rRNA contamination when used with the NEXTFLEX® Poly(A) Beads 2.0. This kit includes reverse transcriptase, necessary library preparation reagents, and cleanup/size selection beads optimized to ensure reliable performance. The kit involves a simple library preparation protocol that has been validated with the updated NEXTFLEX® poly(A) beads 2.0 to accommodate total RNA as input. The NEXTFLEX® rapid directional RNA-seq kit 2.0 is designed to be used with NEXTFLEX® barcodes, which will be provided to early access users. All NEXTFLEX® barcodes are color-balanced and have undergone proprietary purity QC metrics to give you reliable sequencing results for every sample.
BENEFITS:- >> Reliable: Even coverage uniformity with low duplication rate >> Convenient: Optimized with reverse transcriptase and cleanup/size selection beads >> Streamlined: Simple protocol validated with NEXTFLEX® poly(A) beads 2.0 >> Flexible: Designed to work with NEXTFLEX® color-balanced barcodes that allow a wide range of multiplexing (up to 384 samples in one run) >> Efficient: Automated on the Sciclone® G3 NGSx and Zephyr® G3 NGS workstations
Application Details:
Please email tech@nktscientific to request a copy of the datasheet
Catalogue Number Update:
Please note, the catalogue number of this item has changed to include the prefix NOVA-. The original catalogue number (without the "NOVA-" prefix) will still be valid, and the item shipped will be the same as only the catalogue number has changed. The original catalogue number was 5138-08
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