NEXTFLEX® RNA-Seq 2.0 Unique Dual Index Adapters (Set of 1,536)
1,536 RNA-seq UDI barcodes for super high multiplexing capacity with Illumina® paired-end or single-read sequencing Every lot is functionally validated for index purity by sequencing Ideal for Illumina® NovaSeq® runs or projects UDI adapters with 10 nt indexes
Ultra High Sample Multiplexing Options for RNA-Seq on Illumina® Sequencing
This set of 1,536 NEXTFLEX® RNA-Seq 2.0 UDI barcodes (10-nt index) are plated at 6.25 µM stock concentration to be used with the NEXTFLEX® Rapid Directional RNA-Seq kit 2.0 or other RNA-Seq library preparation kits which ligates adapters for Illumina® sequencing to adenylated fragments using similar adapter concentrations.
A set of up to 384 NEXTFLEX® RNA-seq 2.0 UDI barcodes are also available for labs with lower throughput needs.
Reliably Mitigate Index Hopping for Improved Data Integrity
Designed with a hamming distance of at least three throughout the entire series that allows for error correction, the NEXTFLEX® Unique Dual Index barcodes contain two unique indexes on each adapter. These NEXTFLEX® Unique Dual Index RNA-seq 2.0 barcodes can be used for PCR-free RNA sequencing applications as well. If using the Illumina® NovaSeq®, HiSeq® 3000/4000, and HiSeq® X platforms that utilize patterned flow cells, these barcodes would be useful to counter the known increased rates of sample mis-assignment that is thought to occur during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity. Every lot is functionally validated and tested for index purity by sequencing.
KIT CONTENTS
NEXTFLEX® Unique Dual Index Barcodes* (6.25 µM) NEXTFLEX® Primer Mix** (50 µM)
The UDI barcodes are oriented column-wise on each plate (1-8, 9-16, etc).
* These Unique Dual Index barcodes are supplied in duplex form. Do not heat the adapters above room temperature. ** The Primer Mix 2.0 is only intended for use with the NEXTFLEX® Rapid RNA-seq kit 2.0; if the primer mix is to be used with any other library kits of choice, additional dilutions may be required. For additional guidance, please inquire at NGS@perkinelmer.com.
For availability of single use plates for automation, please inquire for additional details.
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Even Coverage Uniformity, Low Duplication Rates, >99% Strand Specificity
The NEXTflex rapid directional RNA-seq kit 2.0 produces libraries for Illumina sequencing with even coverage uniformity, low duplication rates, >99% strand specificity and minimal rRNA contamination when used with the NEXTflex Poly(A) Beads 2.0. This kit includes reverse transcriptase, necessary library preparation reagents, and cleanup/size selection beads optimized to ensure reliable performance. The kit involves a simple library preparation protocol that has been validated with the updated NEXTflex poly(A) beads 2.0 to accommodate total RNA as input. The NEXTflex rapid directional RNA-seq kit 2.0 is designed to be used with NEXTflex barcodes, which will be provided to early access users. All NEXTflex barcodes are color-balanced and have undergone proprietary purity QC metrics to give you reliable sequencing results for every sample.
BENEFITS:- >> Reliable: Even coverage uniformity with low duplication rate >> Convenient: Optimized with reverse transcriptase and cleanup/size selection beads >> Streamlined: Simple protocol validated with NEXTflex poly(A) beads 2.0 >> Flexible: Designed to work with NEXTflex color-balanced barcodes that allow a wide range of multiplexing (up to 384 samples in one run) >> Efficient: Automated on the Sciclone G3 NGSx and Zephyr G3 NGS workstations
The NEXTFLEX® rapid directional RNA-seq kit 2.0 produces libraries for Illumina® sequencing instruments with high coverage uniformity, low duplication rates, strand specificity and minimal rRNA contamination when used with the NEXTFLEX® Poly(A) Beads 2.0 (10 ng 5 ?g) or NEXTFLEX® RiboNaut rRNA depletion kit (human, mouse, rat) (5 ng 1 ?g). This kit includes reverse transcriptase, necessary library preparation reagents, and cleanup/size selection beads optimized to ensure reliable performance.
The kit involves a simple library preparation protocol that has been validated with the updated NEXTFLEX® poly(A) beads 2.0 and NEXTFLEX® RiboNaut rRNA depletion kit (human, mouse, rat) to accommodate total RNA as input. The NEXTFLEX® rapid directional RNA-seq kit 2.0 is designed to be used with NEXTFLEX® RNA-Seq 2.0 Unique Dual Index Barcodes (6.25?M), which are color-balanced and have undergone proprietary purity QC metrics to generate reliable sequencing results for every sample. For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Reliable: High coverage uniformity with low duplication rate Convenient: Optimized for use with 5 ng 5 µg total RNA with reverse transcriptase and cleanup/size selection beads included Streamlined: Simple protocol validated with NEXTFLEX® poly(A) beads 2.0 and NEXTFLEX® RiboNaut rRNA depletion kit (human, mouse, rat) Flexible: Designed to work with NEXTFLEX® RNA-Seq 2.0 Unique Dual Index barcodes that allow a wide range of multiplexing (2 up to 1,526 samples in one run) Efficient: Automated on the Sciclone® G3 NGSx and Zephyr® G3 NGS workstations
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Even Coverage Uniformity, Low Duplication Rates, >99% Strand Specificity
The NEXTflex rapid directional RNA-seq kit 2.0 produces libraries for Illumina sequencing with even coverage uniformity, low duplication rates, >99% strand specificity and minimal rRNA contamination when used with the NEXTflex Poly(A) Beads 2.0. This kit includes reverse transcriptase, necessary library preparation reagents, and cleanup/size selection beads optimized to ensure reliable performance. The kit involves a simple library preparation protocol that has been validated with the updated NEXTflex poly(A) beads 2.0 to accommodate total RNA as input. The NEXTflex rapid directional RNA-seq kit 2.0 is designed to be used with NEXTflex barcodes, which will be provided to early access users. All NEXTflex barcodes are color-balanced and have undergone proprietary purity QC metrics to give you reliable sequencing results for every sample.
BENEFITS:- >> Reliable: Even coverage uniformity with low duplication rate >> Convenient: Optimized with reverse transcriptase and cleanup/size selection beads >> Streamlined: Simple protocol validated with NEXTflex poly(A) beads 2.0 >> Flexible: Designed to work with NEXTflex color-balanced barcodes that allow a wide range of multiplexing (up to 384 samples in one run) >> Efficient: Automated on the Sciclone G3 NGSx and Zephyr G3 NGS workstations
The NEXTFLEX® rapid directional RNA-seq kit 2.0 produces libraries for Illumina® sequencing instruments with high coverage uniformity, low duplication rates, strand specificity and minimal rRNA contamination when used with the NEXTFLEX® Poly(A) Beads 2.0 (10 ng 5 ?g) or NEXTFLEX® RiboNaut rRNA depletion kit (human, mouse, rat) (5 ng 1 ?g). This kit includes reverse transcriptase, necessary library preparation reagents, and cleanup/size selection beads optimized to ensure reliable performance.
The kit involves a simple library preparation protocol that has been validated with the updated NEXTFLEX® poly(A) beads 2.0 and NEXTFLEX® RiboNaut rRNA depletion kit (human, mouse, rat) to accommodate total RNA as input. The NEXTFLEX® rapid directional RNA-seq kit 2.0 is designed to be used with NEXTFLEX® RNA-Seq 2.0 Unique Dual Index Barcodes (6.25?M), which are color-balanced and have undergone proprietary purity QC metrics to generate reliable sequencing results for every sample. For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Reliable: High coverage uniformity with low duplication rate Convenient: Optimized for use with 5 ng 5 µg total RNA with reverse transcriptase and cleanup/size selection beads included Streamlined: Simple protocol validated with NEXTFLEX® poly(A) beads 2.0 and NEXTFLEX® RiboNaut rRNA depletion kit (human, mouse, rat) Flexible: Designed to work with NEXTFLEX® RNA-Seq 2.0 Unique Dual Index barcodes that allow a wide range of multiplexing (2 up to 1,526 samples in one run) Efficient: Automated on the Sciclone® G3 NGSx and Zephyr® G3 NGS workstations
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Even Coverage Uniformity, Low Duplication Rates, >99% Strand Specificity
The NEXTflex rapid directional RNA-seq kit 2.0 produces libraries for Illumina sequencing with even coverage uniformity, low duplication rates, >99% strand specificity and minimal rRNA contamination when used with the NEXTflex Poly(A) Beads 2.0. This kit includes reverse transcriptase, necessary library preparation reagents, and cleanup/size selection beads optimized to ensure reliable performance. The kit involves a simple library preparation protocol that has been validated with the updated NEXTflex poly(A) beads 2.0 to accommodate total RNA as input. The NEXTflex rapid directional RNA-seq kit 2.0 is designed to be used with NEXTflex barcodes, which will be provided to early access users. All NEXTflex barcodes are color-balanced and have undergone proprietary purity QC metrics to give you reliable sequencing results for every sample.
BENEFITS:- >> Reliable: Even coverage uniformity with low duplication rate >> Convenient: Optimized with reverse transcriptase and cleanup/size selection beads >> Streamlined: Simple protocol validated with NEXTflex poly(A) beads 2.0 >> Flexible: Designed to work with NEXTflex color-balanced barcodes that allow a wide range of multiplexing (up to 384 samples in one run) >> Efficient: Automated on the Sciclone G3 NGSx and Zephyr G3 NGS workstations
The NEXTFLEX® rapid directional RNA-seq kit 2.0 produces libraries for Illumina® sequencing instruments with high coverage uniformity, low duplication rates, strand specificity and minimal rRNA contamination when used with the NEXTFLEX® Poly(A) Beads 2.0 (10 ng 5 ?g) or NEXTFLEX® RiboNaut rRNA depletion kit (human, mouse, rat) (5 ng 1 ?g). This kit includes reverse transcriptase, necessary library preparation reagents, and cleanup/size selection beads optimized to ensure reliable performance.
The kit involves a simple library preparation protocol that has been validated with the updated NEXTFLEX® poly(A) beads 2.0 and NEXTFLEX® RiboNaut rRNA depletion kit (human, mouse, rat) to accommodate total RNA as input. The NEXTFLEX® rapid directional RNA-seq kit 2.0 is designed to be used with NEXTFLEX® RNA-Seq 2.0 Unique Dual Index Barcodes (6.25?M), which are color-balanced and have undergone proprietary purity QC metrics to generate reliable sequencing results for every sample. For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Reliable: High coverage uniformity with low duplication rate Convenient: Optimized for use with 5 ng 5 µg total RNA with reverse transcriptase and cleanup/size selection beads included Streamlined: Simple protocol validated with NEXTFLEX® poly(A) beads 2.0 and NEXTFLEX® RiboNaut rRNA depletion kit (human, mouse, rat) Flexible: Designed to work with NEXTFLEX® RNA-Seq 2.0 Unique Dual Index barcodes that allow a wide range of multiplexing (2 up to 1,526 samples in one run) Efficient: Automated on the Sciclone® G3 NGSx and Zephyr® G3 NGS workstations
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Even Coverage Uniformity, Low Duplication Rates, >99% Strand Specificity
The NEXTflex rapid directional RNA-seq kit 2.0 produces libraries for Illumina sequencing with even coverage uniformity, low duplication rates, >99% strand specificity and minimal rRNA contamination when used with the NEXTflex Poly(A) Beads 2.0. This kit includes reverse transcriptase, necessary library preparation reagents, and cleanup/size selection beads optimized to ensure reliable performance. The kit involves a simple library preparation protocol that has been validated with the updated NEXTflex poly(A) beads 2.0 to accommodate total RNA as input. The NEXTflex rapid directional RNA-seq kit 2.0 is designed to be used with NEXTflex barcodes, which will be provided to early access users. All NEXTflex barcodes are color-balanced and have undergone proprietary purity QC metrics to give you reliable sequencing results for every sample.
BENEFITS:- >> Reliable: Even coverage uniformity with low duplication rate >> Convenient: Optimized with reverse transcriptase and cleanup/size selection beads >> Streamlined: Simple protocol validated with NEXTflex poly(A) beads 2.0 >> Flexible: Designed to work with NEXTflex color-balanced barcodes that allow a wide range of multiplexing (up to 384 samples in one run) >> Efficient: Automated on the Sciclone G3 NGSx and Zephyr G3 NGS workstations
The NEXTFLEX® rapid directional RNA-seq kit 2.0 produces libraries for Illumina® sequencing instruments with high coverage uniformity, low duplication rates, strand specificity and minimal rRNA contamination when used with the NEXTFLEX® Poly(A) Beads 2.0 (10 ng 5 ?g) or NEXTFLEX® RiboNaut rRNA depletion kit (human, mouse, rat) (5 ng 1 ?g). This kit includes reverse transcriptase, necessary library preparation reagents, and cleanup/size selection beads optimized to ensure reliable performance.
The kit involves a simple library preparation protocol that has been validated with the updated NEXTFLEX® poly(A) beads 2.0 and NEXTFLEX® RiboNaut rRNA depletion kit (human, mouse, rat) to accommodate total RNA as input. The NEXTFLEX® rapid directional RNA-seq kit 2.0 is designed to be used with NEXTFLEX® RNA-Seq 2.0 Unique Dual Index Barcodes (6.25?M), which are color-balanced and have undergone proprietary purity QC metrics to generate reliable sequencing results for every sample. For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Reliable: High coverage uniformity with low duplication rate Convenient: Optimized for use with 5 ng 5 µg total RNA with reverse transcriptase and cleanup/size selection beads included Streamlined: Simple protocol validated with NEXTFLEX® poly(A) beads 2.0 and NEXTFLEX® RiboNaut rRNA depletion kit (human, mouse, rat) Flexible: Designed to work with NEXTFLEX® RNA-Seq 2.0 Unique Dual Index barcodes that allow a wide range of multiplexing (2 up to 1,526 samples in one run) Efficient: Automated on the Sciclone® G3 NGSx and Zephyr® G3 NGS workstations
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Even Coverage Uniformity, Low Duplication Rates, >99% Strand Specificity
The NEXTflex rapid directional RNA-seq kit 2.0 produces libraries for Illumina sequencing with even coverage uniformity, low duplication rates, >99% strand specificity and minimal rRNA contamination when used with the NEXTflex Poly(A) Beads 2.0. This kit includes reverse transcriptase, necessary library preparation reagents, and cleanup/size selection beads optimized to ensure reliable performance. The kit involves a simple library preparation protocol that has been validated with the updated NEXTflex poly(A) beads 2.0 to accommodate total RNA as input. The NEXTflex rapid directional RNA-seq kit 2.0 is designed to be used with NEXTflex barcodes, which will be provided to early access users. All NEXTflex barcodes are color-balanced and have undergone proprietary purity QC metrics to give you reliable sequencing results for every sample.
BENEFITS:- >> Reliable: Even coverage uniformity with low duplication rate >> Convenient: Optimized with reverse transcriptase and cleanup/size selection beads >> Streamlined: Simple protocol validated with NEXTflex poly(A) beads 2.0 >> Flexible: Designed to work with NEXTflex color-balanced barcodes that allow a wide range of multiplexing (up to 384 samples in one run) >> Efficient: Automated on the Sciclone G3 NGSx and Zephyr G3 NGS workstations
The NEXTFLEX® rapid directional RNA-seq kit 2.0 produces libraries for Illumina® sequencing instruments with high coverage uniformity, low duplication rates, strand specificity and minimal rRNA contamination when used with the NEXTFLEX® Poly(A) Beads 2.0 (10 ng 5 ?g) or NEXTFLEX® RiboNaut rRNA depletion kit (human, mouse, rat) (5 ng 1 ?g). This kit includes reverse transcriptase, necessary library preparation reagents, and cleanup/size selection beads optimized to ensure reliable performance.
The kit involves a simple library preparation protocol that has been validated with the updated NEXTFLEX® poly(A) beads 2.0 and NEXTFLEX® RiboNaut rRNA depletion kit (human, mouse, rat) to accommodate total RNA as input. The NEXTFLEX® rapid directional RNA-seq kit 2.0 is designed to be used with NEXTFLEX® RNA-Seq 2.0 Unique Dual Index Barcodes (6.25?M), which are color-balanced and have undergone proprietary purity QC metrics to generate reliable sequencing results for every sample. For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available.
[BENEFITS] Reliable: High coverage uniformity with low duplication rate Convenient: Optimized for use with 5 ng 5 µg total RNA with reverse transcriptase and cleanup/size selection beads included Streamlined: Simple protocol validated with NEXTFLEX® poly(A) beads 2.0 and NEXTFLEX® RiboNaut rRNA depletion kit (human, mouse, rat) Flexible: Designed to work with NEXTFLEX® RNA-Seq 2.0 Unique Dual Index barcodes that allow a wide range of multiplexing (2 up to 1,526 samples in one run) Efficient: Automated on the Sciclone® G3 NGSx and Zephyr® G3 NGS workstations
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
Even Coverage Uniformity, Low Duplication Rates, >99% Strand Specificity
The NEXTflex rapid directional RNA-seq kit 2.0 produces libraries for Illumina sequencing with even coverage uniformity, low duplication rates, >99% strand specificity and minimal rRNA contamination when used with the NEXTflex Poly(A) Beads 2.0. This kit includes reverse transcriptase, necessary library preparation reagents, and cleanup/size selection beads optimized to ensure reliable performance. The kit involves a simple library preparation protocol that has been validated with the updated NEXTflex poly(A) beads 2.0 to accommodate total RNA as input. The NEXTflex rapid directional RNA-seq kit 2.0 is designed to be used with NEXTflex barcodes, which will be provided to early access users. All NEXTflex barcodes are color-balanced and have undergone proprietary purity QC metrics to give you reliable sequencing results for every sample.
BENEFITS:- >> Reliable: Even coverage uniformity with low duplication rate >> Convenient: Optimized with reverse transcriptase and cleanup/size selection beads >> Streamlined: Simple protocol validated with NEXTflex poly(A) beads 2.0 >> Flexible: Designed to work with NEXTflex color-balanced barcodes that allow a wide range of multiplexing (up to 384 samples in one run) >> Efficient: Automated on the Sciclone G3 NGSx and Zephyr G3 NGS workstations
Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples intended for use to produce libraries for NGS RNA-Seq experiments. Removal of rRNA avoids the waste of reagents and bioinformatic resources to sequence and align the ~85% of total RNA comprised of rRNA, which is generally not a target of interest in NGS sequencing experiments.
The NEXTflex Poly(A) Beads 2.0 offer improved mRNA yields, lower rRNA contamination, and a more simplified protocol, verified from 10 ng - 5 µg of total RNA for a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep.
Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. These beads have been validated with the new and improved NEXTflex Rapid Directional RNA-Seq Kit 2.0.
The NEXTflex Poly(A) beads 2.0 are an easy and cost-effective method for removing rRNA in RNA-Seq libraries. This product takes advantage of the tract of adenosine residues present at the 3 ends of the vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines (oligo dT) immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.
The NEXTflex Poly(A) Beads 2.0 contains enough material to extract mRNA from 8, 48 or 96 total RNA samples for subsequent RNA-seq library prep or other applications. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at 4°C.
These beads have been validated with the following kits: NEXTflex Directional RNA-Seq Kit NEXTflex Directional RNA-Seq Kit 2.0
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples intended for use to produce libraries for NGS RNA-Seq experiments. Removal of rRNA avoids the waste of reagents and bioinformatic resources to sequence and align the ~85% of total RNA comprised of rRNA, which is generally not a target of interest in NGS sequencing experiments.
The NEXTflex Poly(A) Beads 2.0 offer improved mRNA yields, lower rRNA contamination, and a more simplified protocol, verified from 10 ng - 5 µg of total RNA for a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep.
Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. These beads have been validated with the new and improved NEXTflex Rapid Directional RNA-Seq Kit 2.0.
The NEXTflex Poly(A) beads 2.0 are an easy and cost-effective method for removing rRNA in RNA-Seq libraries. This product takes advantage of the tract of adenosine residues present at the 3 ends of the vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines (oligo dT) immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.
The NEXTflex Poly(A) Beads 2.0 contains enough material to extract mRNA from 8, 48 or 96 total RNA samples for subsequent RNA-seq library prep or other applications. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at 4°C.
These beads have been validated with the following kits: NEXTflex Directional RNA-Seq Kit NEXTflex Directional RNA-Seq Kit 2.0
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples intended for use to produce libraries for NGS RNA-Seq experiments. Removal of rRNA avoids the waste of reagents and bioinformatic resources to sequence and align the ~85% of total RNA comprised of rRNA, which is generally not a target of interest in NGS sequencing experiments.
The NEXTflex Poly(A) Beads 2.0 offer improved mRNA yields, lower rRNA contamination, and a more simplified protocol, verified from 10 ng - 5 µg of total RNA for a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep.
Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. These beads have been validated with the new and improved NEXTflex Rapid Directional RNA-Seq Kit 2.0.
The NEXTflex Poly(A) beads 2.0 are an easy and cost-effective method for removing rRNA in RNA-Seq libraries. This product takes advantage of the tract of adenosine residues present at the 3 ends of the vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines (oligo dT) immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.
The NEXTflex Poly(A) Beads 2.0 contains enough material to extract mRNA from 8, 48 or 96 total RNA samples for subsequent RNA-seq library prep or other applications. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at 4°C.
These beads have been validated with the following kits: NEXTflex Directional RNA-Seq Kit NEXTflex Directional RNA-Seq Kit 2.0
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
Several approaches have been used to remove ribosomal RNA (rRNA) from total RNA samples intended for use to produce libraries for NGS RNA-Seq experiments. Removal of rRNA avoids the waste of reagents and bioinformatic resources to sequence and align the ~85% of total RNA comprised of rRNA, which is generally not a target of interest in NGS sequencing experiments.
The NEXTflex Poly(A) Beads 2.0 offer improved mRNA yields, lower rRNA contamination, and a more simplified protocol, verified from 10 ng - 5 µg of total RNA for a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep.
Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes eliminating the need for precipitation. These beads have been validated with the new and improved NEXTflex Rapid Directional RNA-Seq Kit 2.0.
The NEXTflex Poly(A) beads 2.0 are an easy and cost-effective method for removing rRNA in RNA-Seq libraries. This product takes advantage of the tract of adenosine residues present at the 3 ends of the vast majority of protein-coding mRNAs. Hybridization of these poly(A) tails to tracts of complementary thymidines (oligo dT) immobilized on solid supports allows the retrieval of poly(A)+ RNAs by recovering the solid support along with the hybridized RNA complexes.
The NEXTflex Poly(A) Beads 2.0 contains enough material to extract mRNA from 8, 48 or 96 total RNA samples for subsequent RNA-seq library prep or other applications. The shelf life of all reagents is 12 months when stored properly. All components can safely be stored at 4°C.
These beads have been validated with the following kits: NEXTflex Directional RNA-Seq Kit NEXTflex Directional RNA-Seq Kit 2.0
Background Info:
All products sold by Bioo Scientific are intended for research use only unless otherwise indicated. This product is not intended for diagnostic or drug purposes, or for use in humans.
[BENEFITS]: >>> 10 ng - 5 ug total starting RNA >>> Conserve resources by selecting for mRNA prior to library prep >>> Magnetic bead-based protocol for easier wash step and higher recovery >>> No organic solvents or precipitation step required >>> Validated for NGS library preparation
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